LOCUS       NM_013453                418 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens sperm protein associated with the nucleus, X-linked,
            family member A1 (SPANXA1), mRNA.
ACCESSION   NM_013453 XM_377051
VERSION     NM_013453.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Li,J., Bo,H., Zhu,F., Li,Q., Chen,T., Lei,S. and Fan,L.
  TITLE     Hypomethylated SPANXA1/A2 promotes the metastasis of head and neck
            squamous cell carcinoma
  JOURNAL   Med Oncol 37 (12), 112 (2020)
   PUBMED   33175201
  REMARK    GeneRIF: Hypomethylated SPANXA1/A2 promotes the metastasis of head
            and neck squamous cell carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 418)
  AUTHORS   Hsiao,Y.J., Su,K.Y., Hsu,Y.C., Chang,G.C., Chen,J.S., Chen,H.Y.,
            Hong,Q.S., Hsu,S.C., Kang,P.H., Hsu,C.Y., Ho,B.C., Yang,T.H.,
            Wang,C.Y., Jou,Y.S., Yang,P.C. and Yu,S.L.
  TITLE     SPANXA suppresses EMT by inhibiting c-JUN/SNAI2 signaling in lung
            adenocarcinoma
  JOURNAL   Oncotarget 7 (28), 44417-44429 (2016)
   PUBMED   27323831
  REMARK    GeneRIF: Silencing SPANXA increased c-JUN mRNA expression and
            blockage of c-JUN led to SNAI2 down-regulation. Results clearly
            characterized SPANXA as an EMT inhibitor by suppressing c-JUN-SNAI2
            axis in lung adenocarcinoma.
REFERENCE   4  (bases 1 to 418)
  AUTHORS   Maine,E.A., Westcott,J.M., Prechtl,A.M., Dang,T.T., Whitehurst,A.W.
            and Pearson,G.W.
  TITLE     The cancer-testis antigens SPANX-A/C/D and CTAG2 promote breast
            cancer invasion
  JOURNAL   Oncotarget 7 (12), 14708-14726 (2016)
   PUBMED   26895102
  REMARK    GeneRIF: High SPANXA expression is associated with breast cancer
            invasion.
REFERENCE   5  (bases 1 to 418)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   6  (bases 1 to 418)
  AUTHORS   Westbrook,V.A., Schoppee,P.D., Diekman,A.B., Klotz,K.L.,
            Allietta,M., Hogan,K.T., Slingluff,C.L., Patterson,J.W.,
            Frierson,H.F., Irvin,W.P. Jr., Flickinger,C.J., Coppola,M.A. and
            Herr,J.C.
  TITLE     Genomic organization, incidence, and localization of the SPAN-x
            family of cancer-testis antigens in melanoma tumors and cell lines
  JOURNAL   Clin Cancer Res 10 (1 Pt 1), 101-112 (2004)
   PUBMED   14734458
  REMARK    GeneRIF: Over expression of SPAN-x protein is associated with
            nonlymphatic metastatic melanomas
REFERENCE   7  (bases 1 to 418)
  AUTHORS   Zendman,A.J., Zschocke,J., van Kraats,A.A., de Wit,N.J.,
            Kurpisz,M., Weidle,U.H., Ruiter,D.J., Weiss,E.H. and van
            Muijen,G.N.
  TITLE     The human SPANX multigene family: genomic organization, alignment
            and expression in male germ cells and tumor cell lines
  JOURNAL   Gene 309 (2), 125-133 (2003)
   PUBMED   12758128
REFERENCE   8  (bases 1 to 418)
  AUTHORS   Westbrook,V.A., Diekman,A.B., Naaby-Hansen,S., Coonrod,S.A.,
            Klotz,K.L., Thomas,T.S., Norton,E.J., Flickinger,C.J. and Herr,J.C.
  TITLE     Differential nuclear localization of the cancer/testis-associated
            protein, SPAN-X/CTp11, in transfected cells and in 50% of human
            spermatozoa
  JOURNAL   Biol Reprod 64 (1), 345-358 (2001)
   PUBMED   11133693
REFERENCE   9  (bases 1 to 418)
  AUTHORS   Westbrook,V.A., Diekman,A.B., Klotz,K.L., Khole,V.V., von
            Kap-Herr,C., Golden,W.L., Eddy,R.L., Shows,T.B., Stoler,M.H.,
            Lee,C.Y., Flickinger,C.J. and Herr,J.C.
  TITLE     Spermatid-specific expression of the novel X-linked gene product
            SPAN-X localized to the nucleus of human spermatozoa
  JOURNAL   Biol Reprod 63 (2), 469-481 (2000)
   PUBMED   10906052
  REMARK    GeneRIF: Multi-gene family; cancer-testis antigen; SPAN-X protein
            associated with nuclear envelope of human spermatids and
            spermatozoa.
REFERENCE   10 (bases 1 to 418)
  AUTHORS   Zendman,A.J., Cornelissen,I.M., Weidle,U.H., Ruiter,D.J. and van
            Muijen,G.N.
  TITLE     CTp11, a novel member of the family of human cancer/testis antigens
  JOURNAL   Cancer Res 59 (24), 6223-6229 (1999)
   PUBMED   10626816
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CB115288.1, AF098306.1 and
            AI208372.1.
            
            On Apr 9, 2014 this sequence version replaced NM_013453.2.
            
            Summary: Temporally regulated transcription and translation of
            several testis-specific genes is required to initiate the series of
            molecular and morphological changes in the male germ cell lineage
            necessary for the formation of mature spermatozoa. This gene is a
            member of the SPANX family of cancer/testis-associated genes, which
            are located in a cluster on chromosome X. The SPANX genes encode
            differentially expressed testis-specific proteins that localize to
            various subcellular compartments. This particular gene maps to
            chromosome X in a head-to-head orientation with SPANX family member
            A2, which appears to be a duplication of the A1 locus. The protein
            encoded by this gene targets to the nucleus where it associates
            with nuclear vacuoles and the redundant nuclear envelope. Based on
            its association with these poorly characterized regions of the
            sperm nucleus, this protein provides a biochemical marker to study
            unique structures in spermatazoa while attempting to further define
            its role in spermatogenesis. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370519.4/ ENSP00000359550.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                CB115288.1         1-24
            25-408              AF098306.1         1-384
            409-418             AI208372.1         3-12                c
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq27.2"
     gene            1..418
                     /gene="SPANXA1"
                     /gene_synonym="CT11.1; CT11.3; NAP-X; SPAN-X; SPAN-Xa;
                     SPAN-Xb; SPANX; SPANX-A"
                     /note="sperm protein associated with the nucleus,
                     X-linked, family member A1"
                     /db_xref="GeneID:30014"
                     /db_xref="HGNC:HGNC:11218"
                     /db_xref="MIM:300305"
     exon            1..132
                     /gene="SPANXA1"
                     /gene_synonym="CT11.1; CT11.3; NAP-X; SPAN-X; SPAN-Xa;
                     SPAN-Xb; SPANX; SPANX-A"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..354
                     /gene="SPANXA1"
                     /gene_synonym="CT11.1; CT11.3; NAP-X; SPAN-X; SPAN-Xa;
                     SPAN-Xb; SPANX; SPANX-A"
                     /note="sperm protein associated with the nucleus, X
                     chromosome, family member A1; cancer/testis antigen family
                     11, member 1; nuclear-associated protein SPAN-Xa; SPANX
                     family, member A1; cancer/testis antigen 11.1; SPANX
                     family member A; Sperm protein associated with the nucleus
                     on the X chromosome C; Cancer/testis antigen 11.3;
                     Cancer/testis-associated protein CTp11; Nuclear-associated
                     protein SPAN-Xc"
                     /codon_start=1
                     /product="sperm protein associated with the nucleus on the
                     X chromosome A"
                     /protein_id="NP_038481.2"
                     /db_xref="CCDS:CCDS14674.1"
                     /db_xref="GeneID:30014"
                     /db_xref="HGNC:HGNC:11218"
                     /db_xref="MIM:300305"
                     /translation="MDKQSSAGGVKRSVPCDSNEANEMMPETPTGDSDPQPAPKKMKT
                     SESSTILVVRYRRNFKRTSPEELLNDHARENRINPLQMEEEEFMEIMVEIPAK"
     exon            133..418
                     /gene="SPANXA1"
                     /gene_synonym="CT11.1; CT11.3; NAP-X; SPAN-X; SPAN-Xa;
                     SPAN-Xb; SPANX; SPANX-A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      389..394
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPANXA1"
                     /gene_synonym="CT11.1; CT11.3; NAP-X; SPAN-X; SPAN-Xa;
                     SPAN-Xb; SPANX; SPANX-A"
     polyA_site      408
                     /gene="SPANXA1"
                     /gene_synonym="CT11.1; CT11.3; NAP-X; SPAN-X; SPAN-Xa;
                     SPAN-Xb; SPANX; SPANX-A"
     polyA_site      418
                     /gene="SPANXA1"
                     /gene_synonym="CT11.1; CT11.3; NAP-X; SPAN-X; SPAN-Xa;
                     SPAN-Xb; SPANX; SPANX-A"
ORIGIN      
        1 actgagaaga ttcaaaacct acaaaagcct gccactgaca ttgaagaacc aatatataca
       61 atggacaaac aatccagtgc cggcggggtg aagaggagcg tcccctgtga ttccaacgag
      121 gccaacgaga tgatgccgga gaccccaact ggggactcag acccgcaacc tgctcctaaa
      181 aaaatgaaaa catctgagtc ctcgaccata ctagtggttc gctacaggag gaactttaaa
      241 agaacatctc cagaggaact gctgaatgac cacgcccgag agaacagaat caaccccctc
      301 caaatggagg aggaggaatt catggaaata atggttgaaa tacctgcaaa gtagcaagaa
      361 gctacatctc tcaaccttgg gcaatgaaaa taaagtttga gaagctgatg gctgtgta
//
