LOCUS       NM_001039592            1716 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens sperm associated antigen 8 (SPAG8), transcript variant
            1, mRNA.
ACCESSION   NM_001039592
VERSION     NM_001039592.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Gui,M., Croft,J.T., Zabeo,D., Acharya,V., Kollman,J.M.,
            Burgoyne,T., Hoog,J.L. and Brown,A.
  TITLE     SPACA9 is a lumenal protein of human ciliary singlet and doublet
            microtubules
  JOURNAL   Proc Natl Acad Sci U S A 119 (41), e2207605119 (2022)
   PUBMED   36191189
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 1716)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1716)
  AUTHORS   Corominas,R., Yang,X., Lin,G.N., Kang,S., Shen,Y., Ghamsari,L.,
            Broly,M., Rodriguez,M., Tam,S., Wanamaker,S.A., Fan,C., Yi,S.,
            Tasan,M., Lemmens,I., Kuang,X., Zhao,N., Malhotra,D.,
            Michaelson,J.J., Vacic,V., Calderwood,M.A., Roth,F.P.,
            Tavernier,J., Horvath,S., Salehi-Ashtiani,K., Korkin,D., Sebat,J.,
            Hill,D.E., Hao,T., Vidal,M. and Iakoucheva,L.M.
  TITLE     Protein interaction network of alternatively spliced isoforms from
            brain links genetic risk factors for autism
  JOURNAL   Nat Commun 5, 3650 (2014)
   PUBMED   24722188
  REMARK    Erratum:[Nat Commun. 2023 Feb 2;14(1):569. doi:
            10.1038/s41467-023-36264-y.. PMID: 36732511]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1716)
  AUTHORS   Wu,H., Chen,Y., Miao,S., Zhang,C., Zong,S., Koide,S.S. and Wang,L.
  TITLE     Sperm associated antigen 8 (SPAG8), a novel regulator of activator
            of CREM in testis during spermatogenesis
  JOURNAL   FEBS Lett 584 (13), 2807-2815 (2010)
   PUBMED   20488182
  REMARK    GeneRIF: Results indicate that SPAG8 acts as a regulator of ACT and
            plays an important role in CREM-ACT-mediated gene transcription
            during spermatogenesis.
REFERENCE   6  (bases 1 to 1716)
  AUTHORS   Tang,X., Zhang,J., Cai,Y., Miao,S., Zong,S., Koide,S.S. and Wang,L.
  TITLE     Sperm membrane protein (hSMP-1) and RanBPM complex in the
            microtubule-organizing centre
  JOURNAL   J Mol Med (Berl) 82 (6), 383-388 (2004)
   PUBMED   15014887
REFERENCE   7  (bases 1 to 1716)
  AUTHORS   Zhang,X.D., Miao,S.Y., Wang,L.F., Li,Y., Zong,S.D., Yan,Y.C. and
            Koide,S.S.
  TITLE     Human sperm membrane protein (hSMP-1): a developmental
            testis-specific component during germ cell differentiation
  JOURNAL   Arch Androl 45 (3), 239-246 (2000)
   PUBMED   11111873
REFERENCE   8  (bases 1 to 1716)
  AUTHORS   Wang,H., Miao,S., Chen,D., Wang,L. and Koide,S.S.
  TITLE     Assignment of chromosomal locus and evidence for alternatively
            spliced mRNAs of a human sperm membrane protein (hSMP-1)
  JOURNAL   Biochim Biophys Acta 1447 (1), 119-124 (1999)
   PUBMED   10500252
REFERENCE   9  (bases 1 to 1716)
  AUTHORS   Diekman,A.B. and Herr,J.C.
  TITLE     Sperm antigens and their use in the development of an
            immunocontraceptive
  JOURNAL   Am J Reprod Immunol 37 (1), 111-117 (1997)
   PUBMED   9138444
  REMARK    Review article
REFERENCE   10 (bases 1 to 1716)
  AUTHORS   Liu,Q.Y., Wang,L.F., Miao,S.Y. and Catterall,J.F.
  TITLE     Expression and characterization of a novel human sperm membrane
            protein
  JOURNAL   Biol Reprod 54 (2), 323-330 (1996)
   PUBMED   8788182
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U12978.2, BI757195.1,
            CA314354.1 and AL133410.32.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_001039592.1.
            
            Summary: The correlation of anti-sperm antibodies with cases of
            unexplained infertility implicates a role for these antibodies in
            blocking fertilization. Improved diagnosis and treatment of
            immunologic infertility, as well as identification of proteins for
            targeted contraception, are dependent on the identification and
            characterization of relevant sperm antigens. The protein encoded by
            this gene is recognized by sperm agglutinating antibodies from an
            infertile woman. This protein is localized in germ cells of the
            testis at all stages of spermatogenesis and is localized to the
            acrosomal region of mature spermatozoa. This protein interacts with
            ACT (activator of CREM in testis) and may play a role in CREM (cAMP
            response element modulator)-ACT-mediated gene transcription during
            spermatogenesis. This protein may also play a role in
            spermatogenesis by regulating microtubule formation and cell
            division. Alternatively spliced variants that encode different
            protein isoforms have been described but the full-length sequences
            of only two have been determined. [provided by RefSeq, Jul 2012].
            
            Transcript Variant: This variant (1) includes an alternate exon in
            the 3' coding region, compared to variant 2. The resulting protein
            (isoform 1) is shorter and has a distinct C-terminus, compared to
            isoform 2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: HM005490.1, AK302313.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000396638.7/ ENSP00000379878.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                U12978.2           2-14
            14-614              BI757195.1         1-601
            615-1378            U12978.2           616-1379
            1379-1701           CA314354.1         15-337              c
            1702-1716           AL133410.32        129676-129690       c
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p13.3"
     gene            1..1716
                     /gene="SPAG8"
                     /gene_synonym="BS-84; CILD28; CT142; HSD-1; hSMP-1; SMP1;
                     SPAG3"
                     /note="sperm associated antigen 8"
                     /db_xref="GeneID:26206"
                     /db_xref="HGNC:HGNC:14105"
                     /db_xref="MIM:605731"
     exon            1..159
                     /gene="SPAG8"
                     /gene_synonym="BS-84; CILD28; CT142; HSD-1; hSMP-1; SMP1;
                     SPAG3"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..1573
                     /gene="SPAG8"
                     /gene_synonym="BS-84; CILD28; CT142; HSD-1; hSMP-1; SMP1;
                     SPAG3"
                     /note="isoform 1 is encoded by transcript variant 1; sperm
                     membrane protein 1; sperm membrane protein BS-84;
                     testicular tissue protein Li 177"
                     /codon_start=1
                     /product="sperm-associated antigen 8 isoform 1"
                     /protein_id="NP_001034681.1"
                     /db_xref="CCDS:CCDS43798.1"
                     /db_xref="GeneID:26206"
                     /db_xref="HGNC:HGNC:14105"
                     /db_xref="MIM:605731"
                     /translation="METNESTEGSRSRSRSLDIQPSSEGLGPTSEPFPSSDDSPRSAL
                     AAATAAAAAAASAAAATAAFTTAKAAALSTKTPAPCSEFMEPSSDPSLLGEPCAGPGF
                     THNIAHGSLGFEPVYVSCIAQDTCTTTDHSSNPGPVPGSSSGPVLGSSSGAGHGSGSG
                     SGPGCGSVPGSGSGPGPGSGPGSGPGHGSGSHPGPASGPGPDTGPDSELSPCIPPGFR
                     NLVADRVPNYTSWSQHCPWEPQKQPPWEFLQVLEPGARGLWKPPDIKGKLMVCYETLP
                     RGQCLLYNWEEERATNHLDQVPSMQDGSESFFFRHGHRGLLTMQLKSPMPSSTTQKDS
                     YQPPGNVYWPLRGKREAMLEMLLQHQICKEVQAEQEPTRKLFEVESVTHHDYRMELAQ
                     AGTPAPTKPHDYRQEQPETFWIQRAPQLPGVSNIRTLDTPFRKNCSFSTPVPLSLGKL
                     LPYEPENYPYQLGEISSLPCPGGRLGGGGGRMTPF"
     exon            160..979
                     /gene="SPAG8"
                     /gene_synonym="BS-84; CILD28; CT142; HSD-1; hSMP-1; SMP1;
                     SPAG3"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1154
                     /gene="SPAG8"
                     /gene_synonym="BS-84; CILD28; CT142; HSD-1; hSMP-1; SMP1;
                     SPAG3"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1200
                     /gene="SPAG8"
                     /gene_synonym="BS-84; CILD28; CT142; HSD-1; hSMP-1; SMP1;
                     SPAG3"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1315
                     /gene="SPAG8"
                     /gene_synonym="BS-84; CILD28; CT142; HSD-1; hSMP-1; SMP1;
                     SPAG3"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1378
                     /gene="SPAG8"
                     /gene_synonym="BS-84; CILD28; CT142; HSD-1; hSMP-1; SMP1;
                     SPAG3"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1716
                     /gene="SPAG8"
                     /gene_synonym="BS-84; CILD28; CT142; HSD-1; hSMP-1; SMP1;
                     SPAG3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1675..1680
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPAG8"
                     /gene_synonym="BS-84; CILD28; CT142; HSD-1; hSMP-1; SMP1;
                     SPAG3"
                     /note="hexamer: CATAAA"
     polyA_site      1699
                     /gene="SPAG8"
                     /gene_synonym="BS-84; CILD28; CT142; HSD-1; hSMP-1; SMP1;
                     SPAG3"
                     /note="major polyA site"
ORIGIN      
        1 gccccgcaga ggacttgcct ccaccgccac ctgcaagtcc gcccagctgt acttctgcgc
       61 aggctccagg agttgtttgc tgtctctatg gcaacccagt agctggagtc tgaagatgga
      121 gaccaacgag tctacggagg gatcgcggtc gcggtcgcga tctttagaca tacagcccag
      181 ctccgaagga ctggggccca cttcggaacc gtttccttct tcagatgaca gtcccaggtc
      241 ggccctggca gctgcaaccg cagcagctgc agcggctgca tcagctgctg cagctactgc
      301 agccttcacc actgccaaag cagctgcatt atctacaaag accccagcgc cctgttctga
      361 gttcatggag ccgtcctctg accccagcct tcttggggag ccctgtgcgg gacccggctt
      421 tacccacaat atagcccatg ggagtcttgg ctttgagccc gtctatgttt cctgtattgc
      481 tcaggacact tgcactacaa ctgaccatag ttctaatcct ggccctgttc caggctctag
      541 ctctgggcct gttcttggtt ccagctcagg tgctggccat ggctctggct ctggctctgg
      601 tcctggctgt ggctctgtcc ctggctctgg ctctggtcct ggtcctggct ctggtcctgg
      661 ctctggtcct ggtcatggct ctggctctca tcctggtcct gcctctgggc ctggtccaga
      721 cactggccct gactctgagc tcagcccctg tattcctcca gggttcagaa acctggtggc
      781 agatcgggtc cctaactata cctcctggag tcagcactgc ccctgggagc cccagaaaca
      841 accaccttgg gaatttttgc aagtcttaga accgggtgcc cgaggactat ggaaaccccc
      901 agacattaaa gggaagctta tggtttgcta tgaaactttg ccgcggggcc agtgcctcct
      961 ctacaactgg gaggaagaga gagccaccaa ccacctggat caagtcccaa gcatgcagga
     1021 tggctctgag agttttttct tccgacacgg acaccgggga ctgctgacta tgcaactaaa
     1081 gtcacccatg ccctccagca ccacccagaa agactcgtac cagccaccag gaaacgtcta
     1141 ttggccactt cgagggaagc gtgaagccat gctggagatg ctcctgcagc atcagatctg
     1201 taaagaggtg caggcagaac aggaacccac aaggaagctc ttcgaggttg agtctgtgac
     1261 acaccatgac taccgaatgg agctggcaca agcagggact cctgccccaa caaagcctca
     1321 cgactaccgc caggagcaac ctgagacctt ctggatacag agggcaccac agctgccggg
     1381 tgtcagtaac atcaggacat tggacacacc attccggaag aactgcagct tctcaacacc
     1441 agtacccttg tctctgggga aacttttgcc ctatgaacct gagaattacc cctaccaatt
     1501 gggagaaata tcttcccttc cctgtcccgg aggaaggctg ggtggtggag gggggagaat
     1561 gactcctttc tgaggggtga ggagggaagt ggggtatgga atatggaatc tatttctgtc
     1621 tgcactagag aggtcgggag gaagttaatt ctcactgtac ttgaagaggc tttacataaa
     1681 gggttctctc tcatcccaag actgctaatt tagtga
//
