LOCUS       NM_006461               3786 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens sperm associated antigen 5 (SPAG5), mRNA.
ACCESSION   NM_006461
VERSION     NM_006461.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Pang,Y., Liu,Y., Chen,S., Zhang,J., Zeng,J., Pan,Y. and An,J.
  TITLE     [Biological role of SPAG5 in the malignant proliferation of gastric
            cancer cells]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 44 (8), 1497-1507 (2024)
   PUBMED   39276045
  REMARK    GeneRIF: [Biological role of SPAG5 in the malignant proliferation
            of gastric cancer cells].
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Guo,L., Yuan,H., Zhu,H., Zhou,J., Wan,Z. and Zhou,Y.
  TITLE     SPAG5 deficiency activates autophagy to reduce atherosclerotic
            plaque formation in ApoE-/- mice
  JOURNAL   BMC Cardiovasc Disord 24 (1), 275 (2024)
   PUBMED   38807081
  REMARK    GeneRIF: SPAG5 deficiency activates autophagy to reduce
            atherosclerotic plaque formation in ApoE[-/-] mice.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3786)
  AUTHORS   Zhang,X., Wu,W., Li,X., He,F. and Zhang,L.
  TITLE     SPAG5 promotes the proliferation, migration, invasion, and
            epithelial-mesenchymal transformation of colorectal cancer cells by
            activating the PI3K/AKT signaling pathway
  JOURNAL   Chin J Physiol 66 (5), 365-371 (2023)
   PUBMED   37929348
  REMARK    GeneRIF: SPAG5 promotes the proliferation, migration, invasion, and
            epithelial-mesenchymal transformation of colorectal cancer cells by
            activating the PI3K/AKT signaling pathway.
REFERENCE   4  (bases 1 to 3786)
  AUTHORS   Li,H., Qin,Y., Huang,Y., Wang,J. and Ren,B.
  TITLE     SPAG5, the upstream protein of Wnt and the target of curcumin,
            inhibits hepatocellular carcinoma
  JOURNAL   Oncol Rep 50 (3) (2023)
   PUBMED   37539742
  REMARK    GeneRIF: SPAG5, the upstream protein of Wnt and the target of
            curcumin, inhibits hepatocellular carcinoma.
REFERENCE   5  (bases 1 to 3786)
  AUTHORS   Kim,D.K., Weller,B., Lin,C.W., Sheykhkarimli,D., Knapp,J.J.,
            Dugied,G., Zanzoni,A., Pons,C., Tofaute,M.J., Maseko,S.B.,
            Spirohn,K., Laval,F., Lambourne,L., Kishore,N., Rayhan,A.,
            Sauer,M., Young,V., Halder,H., la Rosa,N.M., Pogoutse,O.,
            Strobel,A., Schwehn,P., Li,R., Rothballer,S.T., Altmann,M.,
            Cassonnet,P., Cote,A.G., Vergara,L.E., Hazelwood,I., Liu,B.B.,
            Nguyen,M., Pandiarajan,R., Dohai,B., Coloma,P.A.R., Poirson,J.,
            Giuliana,P., Willems,L., Taipale,M., Jacob,Y., Hao,T., Hill,D.E.,
            Brun,C., Twizere,J.C., Krappmann,D., Heinig,M., Falter,C., Aloy,P.,
            Demeret,C., Vidal,M., Calderwood,M.A., Roth,F.P. and
            Falter-Braun,P.
  TITLE     A proteome-scale map of the SARS-CoV-2-human contactome
  JOURNAL   Nat Biotechnol 41 (1), 140-149 (2023)
   PUBMED   36217029
REFERENCE   6  (bases 1 to 3786)
  AUTHORS   Yang,Y.C., Hsu,Y.T., Wu,C.C., Chen,H.T. and Chang,M.S.
  TITLE     Silencing of astrin induces the p53-dependent apoptosis by
            suppression of HPV18 E6 expression and sensitizes cells to
            paclitaxel treatment in HeLa cells
  JOURNAL   Biochem Biophys Res Commun 343 (2), 428-434 (2006)
   PUBMED   16546135
  REMARK    GeneRIF: These findings suggest that the silencing of astrin induce
            a p53-dependent apoptosis and has an additive effect on paclitaxel
            treatment.
REFERENCE   7  (bases 1 to 3786)
  AUTHORS   Le,X.F., Lammayot,A., Gold,D., Lu,Y., Mao,W., Chang,T., Patel,A.,
            Mills,G.B. and Bast,R.C. Jr.
  TITLE     Genes affecting the cell cycle, growth, maintenance, and drug
            sensitivity are preferentially regulated by anti-HER2 antibody
            through phosphatidylinositol 3-kinase-AKT signaling
  JOURNAL   J Biol Chem 280 (3), 2092-2104 (2005)
   PUBMED   15504738
REFERENCE   8  (bases 1 to 3786)
  AUTHORS   Gruber,J., Harborth,J., Schnabel,J., Weber,K. and Hatzfeld,M.
  TITLE     The mitotic-spindle-associated protein astrin is essential for
            progression through mitosis
  JOURNAL   J Cell Sci 115 (Pt 21), 4053-4059 (2002)
   PUBMED   12356910
REFERENCE   9  (bases 1 to 3786)
  AUTHORS   Mack,G.J. and Compton,D.A.
  TITLE     Analysis of mitotic microtubule-associated proteins using mass
            spectrometry identifies astrin, a spindle-associated protein
  JOURNAL   Proc Natl Acad Sci U S A 98 (25), 14434-14439 (2001)
   PUBMED   11724960
REFERENCE   10 (bases 1 to 3786)
  AUTHORS   Chang,M.S., Huang,C.J., Chen,M.L., Chen,S.T., Fan,C.C., Chu,J.M.,
            Lin,W.C. and Yang,Y.C.
  TITLE     Cloning and characterization of hMAP126, a new member of mitotic
            spindle-associated proteins
  JOURNAL   Biochem Biophys Res Commun 287 (1), 116-121 (2001)
   PUBMED   11549262
  REMARK    GeneRIF: hMap126 is abundantly expressed in testis and localized in
            mitotic spindle and may be involved in the functional and dynamic
            regulation of mitotic spindles.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI459711.1, BC000322.2 and
            BQ773438.1.
            
            On Nov 23, 2018 this sequence version replaced NM_006461.3.
            
            Summary: This gene encodes a protein associated with the mitotic
            spindle apparatus. The encoded protein may be involved in the
            functional and dynamic regulation of mitotic spindles. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF063308.1, SRR1660807.158831.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000321765.10/ ENSP00000323300.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-287               BI459711.1         17-303
            288-3784            BC000322.2         261-3757
            3785-3786           BQ773438.1         26-27               c
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q11.2"
     gene            1..3786
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /note="sperm associated antigen 5"
                     /db_xref="GeneID:10615"
                     /db_xref="HGNC:HGNC:13452"
                     /db_xref="MIM:615562"
     exon            1..130
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     CDS             80..3661
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /note="mitotic spindle coiled-coil related protein;
                     astrin; mitotic spindle associated protein p126"
                     /codon_start=1
                     /product="sperm-associated antigen 5"
                     /protein_id="NP_006452.3"
                     /db_xref="CCDS:CCDS32594.1"
                     /db_xref="GeneID:10615"
                     /db_xref="HGNC:HGNC:13452"
                     /db_xref="MIM:615562"
                     /translation="MWRVKKLSLSLSPSPQTGKPSMRTPLRELTLQPGALTNSGKRSP
                     ACSSLTPSLCKLGLQEGSNNSSPVDFVNNKRTDLSSEHFSHSSKWLETCQHESDEQPL
                     DPIPQISSTPKTSEEAVDPLGNYMVKTIVLVPSPLGQQQDMIFEARLDTMAETNSISL
                     NGPLRTDDLVREEVAPCMGDRFSEVAAVSEKPIFQESPSHLLEESPPNPCSEQLHCSK
                     ESLSSRTEAVREDLVPSESNAFLPSSVLWLSPSTALAADFRVNHVDPEEEIVEHGAME
                     EREMRFPTHPKESETEDQALVSSVEDILSTCLTPNLVEMESQEAPGPAVEDVGRILGS
                     DTESWMSPLAWLEKGVNTSVMLENLRQSLSLPSMLRDAAIGTTPFSTCSVGTWFTPSA
                     PQEKSTNTSQTGLVGTKHSTSETEQLLCGRPPDLTALSRHDLEDNLLSSLVILEVLSR
                     QLRDWKSQLAVPHPETQDSSTQTDTSHSGITNKLQHLKESHEMGQALQQARNVMQSWV
                     LISKELISLLHLSLLHLEEDKTTVSQESRRAETLVCCCFDLLKKLRAKLQSLKAEREE
                     ARHREEMALRGKDAAEIVLEAFCAHASQRISQLEQDLASMREFRGLLKDAQTQLVGLH
                     AKQEELVQQTVSLTSTLQQDWRSMQLDYTTWTALLSRSRQLTEKLTVKSQQALQERDV
                     AIEEKQEVSRVLEQVSAQLEECKGQTEQLELENSRLATDLRAQLQILANMDSQLKELQ
                     SQHTHCAQDLAMKDELLCQLTQSNEEQAAQWQKEEMALKHMQAELQQQQAVLAKEVRD
                     LKETLEFADQENQVAHLELGQVECQLKTTLEVLRERSLQCENLKDTVENLTAKLASTI
                     ADNQEQDLEKTRQYSQKLGLLTEQLQSLTLFLQTKLKEKTEQETLLLSTACPPTQEHP
                     LPNDRTFLGSILTAVADEEPESTPVPLLGSDKSAFTRVASMVSLQPAETPGMEESLAE
                     MSIMTTELQSLCSLLQESKEEAIRTLQRKICELQARLQAQEEQHQEVQKAKEADIEKL
                     NQALCLRYKNEKELQEVIQQQNEKILEQIDKSGELISLREEVTHLTRSLRRAETETKV
                     LQEALAGQLDSNCQPMATNWIQEKVWLSQEVDKLRVMFLEMKNEKEKLMIKFQSHRNI
                     LEENLRRSDKELEKLDDIVQHIYKTLLSIPEVVRGCKELQGLLEFLS"
     exon            131..256
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            257..1341
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1516
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            1517..1591
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            1592..1684
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1819
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            1820..1939
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            1940..2032
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            2033..2146
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            2147..2240
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            2241..2388
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            2389..2491
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            2492..2625
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            2626..2764
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            2765..2876
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            2877..2963
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            2964..3084
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            3085..3196
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            3197..3277
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            3278..3433
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            3434..3508
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            3509..3589
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     exon            3590..3786
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3767..3772
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /note="hexamer: AATAAA"
     polyA_site      3786
                     /gene="SPAG5"
                     /gene_synonym="DEEPEST; hMAP126; MAP126"
                     /note="major polyA site"
ORIGIN      
        1 aggttcaaac acagacggcg ggtgaacatg gcgtcctcga cttggtctga gacgtgatag
       61 gcctgccttc tggttgaaga tgtggcgagt gaaaaaactg agcctcagcc tgtcgccttc
      121 gccccagacg ggaaaaccat ctatgagaac tcctctccgt gaacttaccc tgcagcccgg
      181 tgccctcacc aactctggaa aaagatcccc cgcttgctcc tcgctgaccc catcactgtg
      241 caagctgggg ctgcaggaag gcagcaacaa ctcatctcca gtggattttg taaataacaa
      301 gaggacagac ttatcttcag aacatttcag tcattcctca aagtggctag aaacttgtca
      361 gcatgaatca gatgagcagc ctctagatcc aattccccaa attagctcta ctcctaaaac
      421 gtctgaggaa gcagtagacc cactgggcaa ttatatggtt aaaaccatcg tccttgtacc
      481 atctccactg gggcagcaac aagacatgat atttgaggcc cgtttagata ccatggcaga
      541 gacaaacagc atatctttaa atggaccttt gagaacagac gatctggtga gagaggaggt
      601 ggcaccctgc atgggagaca ggttttcaga agttgctgct gtatctgaga aacctatctt
      661 tcaggaatct ccgtcccatc tcttagagga gtctccacca aatccctgtt ctgaacaact
      721 acattgctcc aaggaaagcc tgagcagtag aactgaggct gtgcgtgagg acttagtacc
      781 ttctgaaagt aacgccttct tgccttcctc tgttctctgg ctttcccctt caactgcctt
      841 ggcagcagat ttccgtgtca atcatgtgga cccagaggag gaaattgtag agcatggagc
      901 tatggaggaa agagaaatga ggtttcccac acatcctaag gagtctgaaa cagaagatca
      961 agcacttgtc tcaagtgtgg aagatattct gtccacatgc ctgacaccaa atctagtaga
     1021 aatggaatcc caagaagctc caggcccagc agtagaagat gttggtagga ttcttggctc
     1081 tgatacagag tcttggatgt ccccactggc ctggctggaa aaaggtgtaa atacctccgt
     1141 catgctggaa aatctccgcc aaagcttatc ccttccctcg atgcttcggg atgctgcaat
     1201 tggcactacc cctttctcta cttgctcggt ggggacttgg tttactcctt cagcaccaca
     1261 ggaaaagagt acaaacacat cccagacagg cctggttggc accaagcaca gtacttctga
     1321 gacagagcag ctcctgtgtg gccggcctcc agatctgact gccttgtctc gacatgactt
     1381 ggaagataac ctgctgagct ctcttgtcat tctggaggtt ctctcccgcc agcttcggga
     1441 ctggaagagc cagctggctg tccctcaccc agaaacccag gacagtagca cacagactga
     1501 cacatctcac agtgggataa ctaataaact tcagcatctt aaggagagcc atgagatggg
     1561 acaggcccta cagcaggcca gaaatgtcat gcaatcatgg gtgcttatct ctaaagagct
     1621 gatatccttg cttcacctat ccctgttgca tttagaagaa gataagacta ctgtgagtca
     1681 ggagtctcgg cgtgcagaaa cattggtctg ttgctgtttt gatttgctga agaaattgag
     1741 ggcaaagctc cagagcctca aagcagaaag ggaggaggca aggcacagag aggaaatggc
     1801 tctcagaggc aaggatgcgg cagagatagt gttggaggct ttctgtgcac acgccagcca
     1861 gcgcatcagc cagctggaac aggacctagc atccatgcgg gaattcagag gccttctgaa
     1921 ggatgcccag acccaactgg tagggcttca tgccaagcaa gaagagctgg ttcagcagac
     1981 agtgagtctt acttctacct tgcaacaaga ctggaggtcc atgcaactgg attatacaac
     2041 atggacagct ttgctgagtc ggtcccgaca actcacagag aaactcacag tcaagagcca
     2101 gcaagccctg caggaacgtg atgtggcaat tgaggaaaag caggaggttt ctagggtgct
     2161 ggaacaagtc tctgcccagt tagaggagtg caaaggccaa acagaacaac tggagttgga
     2221 aaacagtcgt ctagcaacag atctccgggc tcagttgcag attctggcca acatggacag
     2281 ccagctaaaa gagctacaga gtcagcatac ccattgtgcc caggacctgg ctatgaagga
     2341 tgagttactc tgccagctta cccagagcaa tgaggagcag gctgctcaat ggcaaaagga
     2401 agagatggca ctaaaacaca tgcaggcaga actgcagcag caacaagctg tcctggccaa
     2461 agaggtgcgg gacctgaaag agaccttgga gtttgcagac caggagaatc aggttgctca
     2521 cctggagctg ggtcaggttg agtgtcaatt gaaaaccaca ctggaagtgc tccgggagcg
     2581 cagcttgcag tgtgagaacc tcaaggacac tgtagagaac ctaacggcta aactggccag
     2641 caccatagca gataaccagg agcaagatct ggagaaaaca cggcagtact ctcaaaagct
     2701 agggctgctg actgagcaac tacagagcct gactctcttt ctacagacaa aactaaagga
     2761 gaagactgaa caagagaccc ttctgctgag tacagcctgt cctcccaccc aggaacaccc
     2821 tctgcctaat gacaggacct tcctgggaag catcttgaca gcagtggcag atgaagagcc
     2881 agaatcaact cctgtgccct tgcttggaag tgacaagagt gctttcaccc gagtagcatc
     2941 aatggtttcc cttcagcccg cagagacccc aggcatggag gagagcctgg cagaaatgag
     3001 tattatgact actgagcttc agagtctttg ttccctgcta caagagtcta aagaagaagc
     3061 catcaggact ctgcagcgaa aaatttgtga gctgcaagct aggctgcagg cccaggaaga
     3121 acagcatcag gaagtccaga aggcaaaaga agcagacata gagaagctga accaggcctt
     3181 gtgcttgcgc tacaagaatg aaaaggagct ccaggaagtg atacagcagc agaatgagaa
     3241 gatcctagaa cagatagaca agagtggcga gctcataagc cttagagagg aggtgaccca
     3301 ccttacccgc tcacttcggc gtgcggagac agagaccaaa gtgctccagg aggccctggc
     3361 aggccagctg gactccaact gccagcctat ggccaccaat tggatccagg agaaagtgtg
     3421 gctctctcag gaggtggaca aactgagagt gatgttcctg gagatgaaaa atgagaagga
     3481 aaaactcatg atcaagttcc agagccatag aaatatccta gaggagaacc ttcggcgctc
     3541 tgacaaggag ttagaaaaac tagatgacat tgttcagcat atttataaga ccctgctctc
     3601 tattccagag gtggtgaggg gatgcaaaga actacaggga ttgctggaat ttctgagcta
     3661 agaaactgaa agccagaatc tgcttcacct ctttttacct gcaatacccc cttaccccaa
     3721 taccaagacc aactggcata gagccaactg agataaatgc tatttaaata aagtgtattt
     3781 aatgaa
//
