LOCUS       NM_206996               7203 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens sperm associated antigen 17 (SPAG17), mRNA.
ACCESSION   NM_206996
VERSION     NM_206996.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Sapao,P., Roberson,E.D.O., Shi,B., Assassi,S., Skaug,B., Lee,F.,
            Naba,A., Perez White,B.E., Cordova-Fletes,C., Tsou,P.S.,
            Sawalha,A.H., Gudjonsson,J.E., Ma,F., Verma,P., Bhattacharyya,D.,
            Carns,M., Strauss,J.F. 3rd, Sicard,D., Tschumperlin,D.J.,
            Champer,M.I., Campagnola,P.J., Teves,M.E. and Varga,J.
  TITLE     Reduced SPAG17 Expression in Systemic Sclerosis Triggers
            Myofibroblast Transition and Drives Fibrosis
  JOURNAL   J Invest Dermatol 143 (2), 284-293 (2023)
   PUBMED   36116512
  REMARK    GeneRIF: Reduced SPAG17 Expression in Systemic Sclerosis Triggers
            Myofibroblast Transition and Drives Fibrosis.
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   Roberson,E.D.O., Carns,M., Cao,L., Aren,K., Goldberg,I.A.,
            Morales-Heil,D.J., Korman,B.D., Atkinson,J.P. and Varga,J.
  TITLE     Alterations of the Primary Cilia Gene SPAG17 and SOX9 Locus
            Noncoding RNAs Identified by RNA-Sequencing Analysis in Patients
            With Systemic Sclerosis
  JOURNAL   Arthritis Rheumatol 75 (1), 108-119 (2023)
   PUBMED   35762854
  REMARK    GeneRIF: Alterations of the Primary Cilia Gene SPAG17 and SOX9
            Locus Noncoding RNAs Identified by RNA-Sequencing Analysis in
            Patients With Systemic Sclerosis.
REFERENCE   3  (bases 1 to 7203)
  AUTHORS   Cordova-Fletes,C., Becerra-Solano,L.E., Rangel-Sosa,M.M.,
            Rivas-Estilla,A.M., Alberto Galan-Huerta,K., Ortiz-Lopez,R.,
            Rojas-Martinez,A., Juarez-Vazquez,C.I. and Garcia-Ortiz,J.E.
  TITLE     Uncommon runs of homozygosity disclose homozygous missense
            mutations in two ciliopathy-related genes (SPAG17 and WDR35) in a
            patient with multiple brain and skeletal anomalies
  JOURNAL   Eur J Med Genet 61 (3), 161-167 (2018)
   PUBMED   29174089
  REMARK    GeneRIF: Homozygous missense mutation in SPAG17 gene is associated
            with multiple congenital anomalies, including brain malformations
            and skeletal dysplasia suggestive of cranioectodermal dysplasia
            ciliopathy.
REFERENCE   4  (bases 1 to 7203)
  AUTHORS   Xu,X., Sha,Y.W., Mei,L.B., Ji,Z.Y., Qiu,P.P., Ji,H., Li,P., Wang,T.
            and Li,L.
  TITLE     A familial study of twins with severe asthenozoospermia identified
            a homozygous SPAG17 mutation by whole-exome sequencing
  JOURNAL   Clin Genet 93 (2), 345-349 (2018)
   PUBMED   28548327
  REMARK    GeneRIF: In silico analysis revealed that R1448Q is a potential
            pathogenic mutation. Immunostaining and western blot assays showed
            that the R1448Q mutation may exert a negative effect on the
            steady-state of the SPAG17 protein. Therefore, SPAG17 may be a new
            pathogenic gene causing Asthenozoospermia (AZS) .
REFERENCE   5  (bases 1 to 7203)
  AUTHORS   Teves,M.E., Nagarkatti-Gude,D.R., Zhang,Z. and Strauss,J.F. 3rd.
  TITLE     Mammalian axoneme central pair complex proteins: Broader roles
            revealed by gene knockout phenotypes
  JOURNAL   Cytoskeleton (Hoboken) 73 (1), 3-22 (2016)
   PUBMED   26785425
  REMARK    Review article
REFERENCE   6  (bases 1 to 7203)
  AUTHORS   Teves,M.E., Sundaresan,G., Cohen,D.J., Hyzy,S.L., Kajan,I.,
            Maczis,M., Zhang,Z., Costanzo,R.M., Zweit,J., Schwartz,Z.,
            Boyan,B.D. and Strauss,J.F. 3rd.
  TITLE     Spag17 deficiency results in skeletal malformations and bone
            abnormalities
  JOURNAL   PLoS One 10 (5), e0125936 (2015)
   PUBMED   26017218
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 7203)
  AUTHORS   Silina,K., Zayakin,P., Kalnina,Z., Ivanova,L., Meistere,I.,
            Endzelins,E., Abols,A., Stengrevics,A., Leja,M., Ducena,K.,
            Kozirovskis,V. and Line,A.
  TITLE     Sperm-associated antigens as targets for cancer immunotherapy:
            expression pattern and humoral immune response in cancer patients
  JOURNAL   J Immunother 34 (1), 28-44 (2011)
   PUBMED   21150711
REFERENCE   8  (bases 1 to 7203)
  AUTHORS   Zhao,J., Li,M., Bradfield,J.P., Zhang,H., Mentch,F.D., Wang,K.,
            Sleiman,P.M., Kim,C.E., Glessner,J.T., Hou,C., Keating,B.J.,
            Thomas,K.A., Garris,M.L., Deliard,S., Frackelton,E.C., Otieno,F.G.,
            Chiavacci,R.M., Berkowitz,R.I., Hakonarson,H. and Grant,S.F.
  TITLE     The role of height-associated loci identified in genome wide
            association studies in the determination of pediatric stature
  JOURNAL   BMC Med Genet 11, 96 (2010)
   PUBMED   20546612
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 7203)
  AUTHORS   Kim,J.J., Lee,H.I., Park,T., Kim,K., Lee,J.E., Cho,N.H., Shin,C.,
            Cho,Y.S., Lee,J.Y., Han,B.G., Yoo,H.W. and Lee,J.K.
  TITLE     Identification of 15 loci influencing height in a Korean population
  JOURNAL   J Hum Genet 55 (1), 27-31 (2010)
   PUBMED   19893584
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
REFERENCE   10 (bases 1 to 7203)
  AUTHORS   Zhang,Z., Jones,B.H., Tang,W., Moss,S.B., Wei,Z., Ho,C.,
            Pollack,M., Horowitz,E., Bennett,J., Baker,M.E. and Strauss,J.F.
            3rd.
  TITLE     Dissecting the axoneme interactome: the mammalian orthologue of
            Chlamydomonas PF6 interacts with sperm-associated antigen 6, the
            mammalian orthologue of Chlamydomonas PF16
  JOURNAL   Mol Cell Proteomics 4 (7), 914-923 (2005)
   PUBMED   15827353
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL513191.4, DB060943.1,
            AY555274.1, DB340825.1 and AL121993.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_206996.3.
            
            Summary: This gene encodes a central pair protein present in the
            axonemes of cells with a '9 + 2' organization of microtubules. The
            encoded protein is required for the proper function of the axoneme.
            Mutations in the orthologous gene in mice lead to primary ciliary
            dyskinesia characterized by immotile nasal and tracheal cilia,
            reduced clearance of nasal mucus, profound respiratory distress,
            hydrocephalus, and neonatal lethality within twelve hours of birth
            due to impaired airway mucociliary clearance. Single-nucleotide
            polymorphisms in this gene are associated with human height and
            targeted mutations lead to skeletal malformations affecting the
            limbs in mice, suggesting a role for this gene in skeletal
            development. [provided by RefSeq, Feb 2017].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY555274.1, BX648110.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1966682,
                                           SAMEA1968189 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000336338.10/ ENSP00000337804.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AL513191.4         152826-152828       c
            4-17                DB060943.1         1-14
            18-7128             AY555274.1         1-7111
            7129-7198           DB340825.1         1-70                c
            7199-7203           AL121993.7         77015-77019         c
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p12"
     gene            1..7203
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /note="sperm associated antigen 17"
                     /db_xref="GeneID:200162"
                     /db_xref="HGNC:HGNC:26620"
                     /db_xref="MIM:616554"
     exon            1..158
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..6743
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /note="projection protein PF6 homolog"
                     /codon_start=1
                     /product="sperm-associated antigen 17"
                     /protein_id="NP_996879.1"
                     /db_xref="CCDS:CCDS899.1"
                     /db_xref="GeneID:200162"
                     /db_xref="HGNC:HGNC:26620"
                     /db_xref="MIM:616554"
                     /translation="MAPKKEKGGTVNTSSKIWEPSLIAAQFNQNDWQASIAFVVGNQI
                     EDDLLIQALTVAVQVPQRKLFSMVSWQDILQQINEINTLVGSASSKKAKKPVGGNAPL
                     YYEVLTAAKAIMDSGEKLTLPLIGKLLKFQLLQIKFKDQQRRENEKKVIEDKPKLEKD
                     KGKAKSPKEKKAPSAKPAKGKGKDQPEANAPVKKTTQLKRRGEDDHTNRYIDDEPDDG
                     AQHYIIVVGFNNPQLLAIMAELGIPITSVIKISSENYEPLQTHLAAVNQQQEVLLQSE
                     DLEAEKLKKENAIKELKTFWKYLEPVLNNEKPETNLFDVARLEYMVKAADFPSDWSDG
                     EMMLKLGTDIFENIACLMYDILDWKRQHQHYLESMQLINVPQVVNEKPVLEAMPTSEA
                     PQPAVPAPGKKKAQYEEPQAPPPVTSVITTEVDMRYYNYLLNPIREEFISVPLILHCM
                     LEQVVATEEDLVPPSLREPSPRADGLDHRIAAHIVSLLPSLCLSEREKKNLHDIFLSE
                     EENESKAVPKGPLLLNYHDAHAHKKYALQDQKNFDPVQIEQEMQSKLPLWEFLQFPLP
                     PPWNNTKRLATIHELMHFCTSDVLSWNEVERAFKVFTFESLKLSEVDEKGKLKPSGMM
                     CGSDSEMFNIPWDNPARFAKQIRQQYVMKMNTQEAKQKADIKIKDRTLFVDQNLSMSV
                     QDNESNREPSDPSQCDANNMKHSDLNNLKLSVPDNRQLLEQESIMKAQPQHESLEQTT
                     NNEIKDDAVTKADSHEKKPKKMMVEADLEDIKKTQQRSLMDWSFTEHFKPKVLLQVLQ
                     EAHKQYRCVDSYYHTQDNSLLLVFHNPMNRQRLHCEYWNIALHSNVGFRNYLELVAKS
                     IQDWITKEEAIYQESKMNEKIIRTRAELELKSSANAKLTSASKIFSIKESKSNKGISK
                     TEISDQEKEKEKEKIPFILEGSLKAWKEEQHRLAEEERLREEKKAEKKGKEAGKKKGK
                     DNAEKEDSRSLKKKSPYKEKSKEEQVKIQEVTEESPHQPEPKITYPFHGYNMGNIPTQ
                     ISGSNYYLYPSDGGQIEVEKTMFEKGPTFIKVRVVKDNHNFMIHLNDPKEIVKKEEKG
                     DYYLEEEEEGDEEQSLETEVSDAKNKAFSKFGSFSATLENGICLSISYYGSNGMAPED
                     KDPDLETILNIPSALTPTVVPVIVTVPQSKAKGKIKGKEKPKESLKEEEHPKEEEKKE
                     EEVEPEPVLQETLDVPTFQSLNVSCPSGLLLTFIGQESTGQYVIDEEPTWDIMVRQSY
                     PQRVKHYEFYKTVMPPAEQEASRVITSQGTVVKYMLDGSTQILFADGAVSRSPNSGLI
                     CPPSEMPATPHSGDLMDSISQQKSETIPSEITNTKKGKSHKSQSSMAHKGEIHDPPPE
                     AVQTVTPVEVHIGTWFTTTPEGNRIGTKGLERIADLTPLLSFQATDPVNGTVMTTRED
                     KVVIVERKDGTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQVKCMRVE
                     SSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHES
                     SSNIYYPFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKKLED
                     DLNEKTEGYDSLSSMHLEKNHQQIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAY
                     KESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVKKEDTIVPPNLRSRSWETFPSVE
                     KKTPGPPFGTQIWKGLCIESKQLVSAPGAILKSPSVLQMRQFIQHEVIKNEVKLRLQV
                     SLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVA
                     AHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYL
                     MDIKNRIIPPFFKSELNQLYQSQYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLD
                     FKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTENLTKSPEEAESYEPVKIPTQSL
                     LQDVAGQTRKEKVKLPHYLLSSKPKSQPLAKVQDSVGGKVNTSSVASAAINNAKSSLF
                     GFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAA
                     GMQTELNIELFATAVGEDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNYDKRPK
                     DFPQGKENPMVQRTSTIYSSTLGVFMSRKVSPH"
     exon            159..299
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            300..386
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            387..518
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            519..705
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            706..900
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1082
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1244
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1317
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..1430
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1568
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1682
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..1833
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..2061
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            2062..2280
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            2281..2342
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            2343..2456
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            2457..2611
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            2612..2793
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            2794..2885
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            2886..3125
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            3126..3237
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            3238..3390
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            3391..3504
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            3505..3680
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            3681..3801
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            3802..3980
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            3981..4140
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            4141..4358
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            4359..4503
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            4504..4658
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            4659..4847
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            4848..4993
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            4994..5124
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            5125..5249
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            5250..5432
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            5433..5546
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            5547..5592
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            5593..5692
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            5693..5740
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            5741..5840
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            5841..5943
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            5944..6075
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            6076..6212
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            6213..6397
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            6398..6458
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            6459..6603
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            6604..6743
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     exon            6744..7203
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6900..6905
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /note="hexamer: AATAAA"
     regulatory      6914..6919
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /note="hexamer: AATAGA"
     polyA_site      6932
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
                     /note="major polyA site"
     polyA_site      6942
                     /gene="SPAG17"
                     /gene_synonym="CT143; PF6; SPGF55"
ORIGIN      
        1 actgtggtag cggggacgct taggcagggc ctgcgcccag tttagaggcc aatgcttctc
       61 ccgtcctttg catggcaccc aagaaggaga aaggaggaac tgtgaacacc agttctaaga
      121 tatgggaacc ctcgctcata gctgcacagt tcaatcagaa cgattggcag gcctccattg
      181 cttttgtggt tgggaaccag attgaagatg atcttctcat ccaagccctt accgtggctg
      241 tccaggtccc tcagcgtaaa ctcttcagta tggtgtcgtg gcaagacatt ctccagcaga
      301 ttaatgaaat aaatacactt gttggatctg cttcatctaa aaaggcaaaa aaacctgtag
      361 gtggtaatgc tcctttatat tatgaggtgt taacggcagc aaaagcaatt atggatagtg
      421 gagagaaatt aaccttacca ctgataggga aactcttgaa atttcaactt ctccagatta
      481 aatttaagga ccaacagcga cgggaaaatg aaaagaaggt aatagaagac aaacctaagt
      541 tagaaaagga taaagggaaa gcaaaatctc ccaaggagaa aaaggctcca agtgccaagc
      601 ctgccaaagg aaagggaaag gatcagcctg aggcaaatgc accagtgaaa aagaccaccc
      661 agttaaagcg gagaggagaa gacgaccaca ccaatcgtta cattgacgat gagccagatg
      721 atggtgccca acattacatt atagttgtgg gctttaacaa tcctcagcta ttagcaatta
      781 tggctgagct tggcattcct ataaccagcg tgattaaaat atcttcagag aattatgaac
      841 ctctgcagac acacctggca gcagttaacc agcagcagga agttcttctt cagtcagaag
      901 atctagaagc agaaaaattg aagaaagaaa atgccataaa agagcttaaa actttctgga
      961 agtacttgga accagtcctg aataatgaga aacctgaaac aaatctcttt gatgttgctc
     1021 gacttgagta catggtcaaa gcagctgatt ttccttctga ctggtcagat ggtgagatga
     1081 tgctgaaatt gggcactgat atttttgaaa atattgcctg cttgatgtat gacatcctgg
     1141 attggaaaag gcagcaccag cactatttgg aaagcatgca gcttattaat gttccacaag
     1201 tggttaatga gaaacctgta ttagaagcca tgccaacttc agaggctcca caacctgctg
     1261 taccagctcc tggaaagaag aaagcacagt atgaagaacc gcaagctcca ccaccagtga
     1321 cttcagtcat cacaactgaa gtagacatga gatattacaa ttatttgctg aatccaattc
     1381 gagaggaatt catttctgtg cccctgatac tgcattgtat gctggaacag gttgttgcaa
     1441 ctgaagaaga tctcgtccca cccagtctgc gggagccatc ccccagagca gacgggctag
     1501 accacagaat cgcagctcac attgtgtccc ttctgccctc actctgtctc tcagagaggg
     1561 agaaaaagaa tcttcatgac atatttttat ctgaagaaga aaatgaaagc aaagcagtgc
     1621 ccaaaggccc cctcctactg aactatcatg atgcacacgc ccacaagaag tacgcactac
     1681 aggaccaaaa gaattttgat ccagttcaaa ttgagcagga gatgcagtcc aagttgccac
     1741 tgtgggaatt tcttcaattc cctctacccc caccatggaa caacactaaa cgtctagcta
     1801 caattcatga gcttatgcac ttttgtacga gtgatgtctt gagctggaat gaagtagaac
     1861 gagccttcaa ggtgtttact tttgagagcc tgaagctctc tgaggttgat gaaaaaggga
     1921 aactgaaacc ttctgggatg atgtgtgggt cagattctga aatgttcaac ataccgtggg
     1981 acaaccctgc cagatttgct aaacagataa ggcagcaata tgtcatgaaa atgaatactc
     2041 aagaggccaa gcagaaagca gatattaaaa tcaaagacag aacactattt gtggatcaga
     2101 atttgtcaat gtctgtgcaa gataatgaaa gcaaccgaga accttcagat cctagtcagt
     2161 gtgatgctaa caatatgaag cattctgact tgaataatct caaactctca gtccctgata
     2221 atagacagct gttagagcag gagagcatca tgaaggctca gccccaacat gagtctctgg
     2281 agcagaccac aaacaatgag atcaaagatg atgcagtcac aaaggctgat tctcatgaaa
     2341 agaaacccaa gaagatgatg gtggaagcag atttagagga cataaagaaa acacagcagc
     2401 gcagtctaat ggactggagt tttactgaac attttaaacc gaaagtactg cttcaggtcc
     2461 ttcaagaagc ccataagcaa tataggtgtg ttgattctta ctaccacacc caagacaact
     2521 ctttactttt agtctttcac aatccaatga atagacaacg tttgcattgt gaatattgga
     2581 acattgctct ccactccaat gttggattca ggaattattt ggaacttgtt gcaaaatcta
     2641 ttcaagattg gattacaaaa gaagaagcta tatatcagga atctaaaatg aatgagaaaa
     2701 tcatcaggac cagagctgag ctggaattga aatcttctgc taatgccaaa cttacttctg
     2761 ctagcaaaat tttttccatt aaagaatcta aaagtaacaa aggaatcagc aaaacagaga
     2821 tatcagatca agaaaaagaa aaagagaagg aaaagattcc tttcatttta gaaggctctc
     2881 tcaaggcatg gaaagaagag caacatcgat tagcagaaga ggagcgctta agggaagaaa
     2941 agaaagcaga gaagaagggt aaagaagctg gtaaaaagaa aggcaaggat aacgcagaga
     3001 aagaggatag taggtctttg aagaaaaaat caccttacaa ggagaaatct aaagaagaac
     3061 aagtcaagat ccaagaagta acagaagagt ccccccacca accagaacct aagataactt
     3121 acccgtttca cggatacaat atgggaaata tacccactca aatctcaggg tcaaattact
     3181 acctgtatcc ttctgatggg gggcagattg aagtggaaaa gacaatgttt gaaaaaggcc
     3241 caacttttat caaagtgaga gtggtaaagg acaaccacaa ttttatgatt catttaaatg
     3301 accctaagga aattgtgaaa aaggaagaga aaggggatta ttatttagaa gaggaagaag
     3361 aaggagatga ggaacaaagt cttgaaacgg aagtatcaga tgcaaagaat aaagctttca
     3421 gcaagtttgg atctttttct gccaccttag aaaatggaat ctgcctctcg ataagttact
     3481 atggatcaaa tggaatggca ccagaagata aggatcctga tttagaaaca atattgaata
     3541 tcccttcagc actcactcca acagtggttc ctgttatagt gaccgttcct caaagcaaag
     3601 ctaaagggaa aataaaaggc aaagaaaaac ccaaagaatc ccttaaagaa gaagaacacc
     3661 caaaagaaga agagaaaaag gaagaagaag tagaaccaga acctgtttta caagagactt
     3721 tggatgttcc caccttccag agcctaaatg tgtcttgccc cagtgggctc ctgttgactt
     3781 tcattggaca agaatctaca ggtcaatatg ttatagatga ggaacccacc tgggacatca
     3841 tggtccgtca gagctacccc cagagggtga agcactatga gttctataaa acggtgatgc
     3901 cacccgcaga gcaggaggct tcaagggtta tcaccagtca aggcactgtt gtcaaatata
     3961 tgttggatgg atccacacag attctctttg cagatggtgc tgtgagcagg agtcccaatt
     4021 caggtcttat ttgtcctcct tctgaaatgc cagcaacgcc tcacagtgga gatttgatgg
     4081 actctatttc tcagcagaaa tcagaaacga taccatctga gattaccaac acaaagaaag
     4141 gaaaaagtca caaaagtcag tcatcaatgg cccataaggg tgaaatccat gaccctcctc
     4201 cagaggcagt tcaaactgta actcctgtgg aggttcacat aggcacctgg tttacaacca
     4261 cacctgaagg aaatcggatc ggcaccaaag gattagaaag aatagcagac ttgaccccat
     4321 tgttatcctt tcaggccaca gatcctgtca atggaacggt tatgacaact cgagaagaca
     4381 aagttgtcat agttgaaagg aaagatggta ctcggatagt ggatcatgct gatggtacca
     4441 gaatcacaac cttttatcaa gtttatgaag atcaaattat tctgccagat gatcaagaaa
     4501 caaccgaggg tcctcggact gtcaccaggc aggtgaagtg tatgcgggta gaaagctcac
     4561 gctatgccac tgttatcgcc aactgtgagg acagtagctg ctgtgccacc tttggagatg
     4621 gaacaactat tattgcaaag ccacagggaa cataccaggt gttacctcca aacacaggct
     4681 ctctttatat tgacaaggat tgttcagctg tgtactgcca tgagtcaagc agtaatatat
     4741 actatccttt tcaaaagcgt gagcagctgc gagctggcag gtacatcatg aggcatactt
     4801 cagaggttat ctgtgaggtt ctggatcctg agggaaacac ttttcaggtc atggctgatg
     4861 gtagcatatc aactatatta cctgaaaaaa aattggaaga tgatttaaat gagaaaactg
     4921 agggctatga tagtctgtcc tctatgcacc ttgaaaagaa tcatcagcaa atctatggtg
     4981 aacatgtccc caggtttttt gttatgtatg ctgatggatc aggaatggaa cttcttcgag
     5041 acagtgacat agaagaatat ctatctttgg catataaaga atcaaatact gttgttctcc
     5101 aagagccagt gcaggaacag ccaggcaccc taaccatcac agtccttcgc cctttccatg
     5161 aagcatcacc atggcaagta aaaaaggaag atacaattgt ccctcctaat ctccggtcaa
     5221 ggtcatggga aacatttccc tcagttgaga aaaaaactcc aggacctccg tttggtactc
     5281 agatttggaa aggcctttgc attgagtcca aacagctagt gagtgccccg ggtgccatac
     5341 tcaagagccc cagtgtgcta cagatgcgcc aattcattca gcatgaggtc ataaagaatg
     5401 aggtgaaact gaggctgcag gtttccctta aggattacat aaactatatt ctaaagaaag
     5461 aagatgagct gcaggaaatg atggttaaag attccagaac tgaggaggag agaggcaatg
     5521 ctgctgatct cctcaagctg gttatgtctt tccctaaaat ggaggaaact acaaaaagtc
     5581 atgttactga agttgcagct cacctaactg atttattcaa gcagtctttg gctacgcctc
     5641 caaaatgccc accagacaca tttggtaaag atttctttga aaagacatgg agacacacag
     5701 catcctcaaa acgctggaaa gaaaagatag acaaaacgag gaaggaaatt gagacaacac
     5761 agaattacct aatggatatt aagaaccgca taataccacc cttttttaaa tctgaattga
     5821 accagttata tcagtctcag tataatcacc tggacagtct ttccaaaaaa ctgccttctt
     5881 ttacaaagaa aaatgaagat gcaaacgaaa cagctgttca agatacatct gatcttaatc
     5941 tagatttcaa gccacataag gtttcagaac agaaatcctc aagtgtgcct agtcttccaa
     6001 aaccagagat ttctgcagat aagaaggatt tcactgctca gaaccaaact gaaaatttaa
     6061 caaaatctcc tgaagaagca gaatcttatg agcccgtgaa aattccaacc cagtccttgc
     6121 tgcaggatgt tgcgggacaa acaagaaaag aaaaagtgaa gttgcctcat tatttgctga
     6181 gttccaagcc taagtctcaa cctcttgcaa aggtgcaaga ttctgttgga ggaaaagtga
     6241 acacatcctc tgttgcatct gctgccatta ataatgcaaa gtcatccctt tttgggttcc
     6301 atcttctccc atcatcagtc aagtttggag tgcttaagga aggacatacc tatgccacag
     6361 ttgtaaagct caagaatgtt ggagtggact tctgcaggtt taaagtaaag cagcccccac
     6421 ccagcacagg actgaaagtg acttacaaac ctggacctgt ggcagctggt atgcagacag
     6481 aactgaatat agagttattt gccacagctg ttggagagga tggggccaag ggatcagcac
     6541 acatctctca caatatcgag attatgacag agcatgaggt tctgttccta cctgtggaag
     6601 caactgtttt aacaagcagc aattatgata aacgaccaaa agactttccc cagggaaaag
     6661 aaaatccaat ggtccagaga acttctacaa tttattcctc cacacttgga gtcttcatgt
     6721 ctcgtaaagt ttctccacat tagtttcttc tcggtacaac tcaatagcct ccataatcct
     6781 ctcagcctac agaggatgag aaaggaaaga agtcatcaca acatactcca tcatcccagg
     6841 acactgaaac tggaagaact gaccagaaat ttgccaaatg aaatagcttc aatctgttta
     6901 ataaagacgt gcgaatagag tgccaaaaag catttaaaga catttttggt tttcttattt
     6961 tttaatgtac agggaagaga ctgagtgtca tggactcagg tgggatgata tctagacact
     7021 ctgaataggg caggtcccag gaacacctga aaggagcaca tattgatcaa agagattcag
     7081 gactactccc atctttggtt acaagagaag gaaacaggtt ataatcccca tctttaaatc
     7141 tcatcaactt tttgccaaaa aaatggcttt ctggttgata cagaataaaa cttttactaa
     7201 aaa
//
