LOCUS       NM_058201                551 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens sperm associated antigen 11B (SPAG11B), transcript
            variant D, mRNA.
ACCESSION   NM_058201
VERSION     NM_058201.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 551)
  AUTHORS   Narmadha,G. and Yenugu,S.
  TITLE     Molecular modeling of the human sperm associated antigen 11 B
            (SPAG11B) proteins
  JOURNAL   Syst Biol Reprod Med 61 (2), 78-88 (2015)
   PUBMED   25573789
  REMARK    GeneRIF: we report the three dimensional structure of the human
            SPAG11B proteins and their C-terminal peptides. web-based
            hydropathy, amphipathicity, and topology (WHAT) analyses and grand
            average of hydropathy
REFERENCE   3  (bases 1 to 551)
  AUTHORS   Drabovich,A.P., Jarvi,K. and Diamandis,E.P.
  TITLE     Verification of male infertility biomarkers in seminal plasma by
            multiplex selected reaction monitoring assay
  JOURNAL   Mol Cell Proteomics 10 (12), M110.004127 (2011)
   PUBMED   21933954
  REMARK    GeneRIF: Combination of two proteins, SPAG11B and TEX101, can be
            used for differential diagnosis of azoospermia
REFERENCE   4  (bases 1 to 551)
  AUTHORS   Radhakrishnan,Y., Hamil,K.G., Tan,J.A., Grossman,G., Petrusz,P.,
            Hall,S.H. and French,F.S.
  TITLE     Novel partners of SPAG11B isoform D in the human male reproductive
            tract
  JOURNAL   Biol Reprod 81 (4), 647-656 (2009)
   PUBMED   19535787
  REMARK    GeneRIF: SPAG11B/D is both a substrate and a potent inhibitor of
            TPSAB1 activity.
REFERENCE   5  (bases 1 to 551)
  AUTHORS   Yenugu,S., Hamil,K.G., French,F.S. and Hall,S.H.
  TITLE     Antimicrobial actions of human and macaque sperm associated antigen
            (SPAG) 11 isoforms: influence of the N-terminal peptide
  JOURNAL   Mol Cell Biochem 284 (1-2), 25-37 (2006)
   PUBMED   16411022
  REMARK    GeneRIF: Isoform C of SPAG11B encodes a peptide with antimicrobial
            activity against E. coli.
REFERENCE   6  (bases 1 to 551)
  AUTHORS   Avellar,M.C., Honda,L., Hamil,K.G., Yenugu,S., Grossman,G.,
            Petrusz,P., French,F.S. and Hall,S.H.
  TITLE     Differential expression and antibacterial activity of epididymis
            protein 2 isoforms in the male reproductive tract of human and
            rhesus monkey (Macaca mulatta)
  JOURNAL   Biol Reprod 71 (5), 1453-1460 (2004)
   PUBMED   15229135
  REMARK    GeneRIF: EP2 expression is more widespread in male reproductive
            tract than realized previously. Activity of every EP2 variant
            tested is antibacterial. Further investigation may reveal
            additional roles for EP2 peptides in primate male reproductive
            tract.
REFERENCE   7  (bases 1 to 551)
  AUTHORS   Hamil,K.G., Sivashanmugam,P., Richardson,R.T., Grossman,G.,
            Ruben,S.M., Mohler,J.L., Petrusz,P., O'Rand,M.G., French,F.S. and
            Hall,S.H.
  TITLE     HE2beta and HE2gamma, new members of an epididymis-specific family
            of androgen-regulated proteins in the human
  JOURNAL   Endocrinology 141 (3), 1245-1253 (2000)
   PUBMED   10698202
REFERENCE   8  (bases 1 to 551)
  AUTHORS   Osterhoff,C., Kirchhoff,C., Krull,N. and Ivell,R.
  TITLE     Molecular cloning and characterization of a novel human sperm
            antigen (HE2) specifically expressed in the proximal epididymis
  JOURNAL   Biol Reprod 50 (3), 516-525 (1994)
   PUBMED   8167223
REFERENCE   9  (bases 1 to 551)
  AUTHORS   Krull,N., Ivell,R., Osterhoff,C. and Kirchhoff,C.
  TITLE     Region-specific variation of gene expression in the human
            epididymis as revealed by in situ hybridization with
            tissue-specific cDNAs
  JOURNAL   Mol Reprod Dev 34 (1), 16-24 (1993)
   PUBMED   8418812
REFERENCE   10 (bases 1 to 551)
  AUTHORS   Kirchhoff,C., Osterhoff,C., Habben,I. and Ivell,R.
  TITLE     Cloning and analysis of mRNAs expressed specifically in the human
            epididymis
  JOURNAL   Int J Androl 13 (2), 155-167 (1990)
   PUBMED   1693137
  REMARK    Erratum:[Int J Androl 1990 Aug;13(4):327. Kirchloff C [corrected to
            Kirchhoff C]]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF168617.1 and AC130360.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 20, 2020 this sequence version replaced NM_058201.3.
            
            Summary: This gene encodes several androgen-dependent,
            epididymis-specific secretory proteins. The specific functions of
            these proteins have not been determined, but they are thought to be
            involved in sperm maturation. Some of the isoforms contain regions
            of similarity to beta-defensins, a family of antimicrobial
            peptides. The gene is located on chromosome 8p23 near the defensin
            gene cluster. Alternative splicing of this gene results in seven
            transcript variants encoding different isoforms. Two different
            N-terminal and five different C-terminal protein sequences are
            encoded by the splice variants. Two additional variants have been
            described, but their full length sequences have not been
            determined. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (D) is also called the beta1
            transcript. It lacks three internal exons. The C-terminus of the
            encoded isoform (D) is identical to that of isoform E encoded by
            variant E. Isoform D has a region with similarity to
            beta-defensins.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189667.404981.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match                 :: ENST00000398462.7/
                                                  ENSP00000381480.2
            Protein has antimicrobial activity :: PMID: 15229135, 16411022
            RefSeq Select criteria             :: based on conservation,
                                                  longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-289               AF168617.1         113-401
            290-551             AC130360.4         138710-138971       c
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p23.1"
     gene            1..551
                     /gene="SPAG11B"
                     /gene_synonym="EDDM2B; EP2; EP2C; EP2D; HE2; HE2C; SPAG11;
                     SPAG11A"
                     /note="sperm associated antigen 11B"
                     /db_xref="GeneID:10407"
                     /db_xref="HGNC:HGNC:14534"
                     /db_xref="MIM:606560"
     exon            1..100
                     /gene="SPAG11B"
                     /gene_synonym="EDDM2B; EP2; EP2C; EP2D; HE2; HE2C; SPAG11;
                     SPAG11A"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..441
                     /gene="SPAG11B"
                     /gene_synonym="EDDM2B; EP2; EP2C; EP2D; HE2; HE2C; SPAG11;
                     SPAG11A"
                     /note="isoform D preproprotein is encoded by transcript
                     variant D; epididymal protein 2B; Sperm-associated antigen
                     11A; Protein EP2; sperm antigen HE2; human
                     epididymis-specific protein 2"
                     /codon_start=1
                     /product="sperm-associated antigen 11B isoform D
                     preproprotein"
                     /protein_id="NP_478108.2"
                     /db_xref="CCDS:CCDS47774.1"
                     /db_xref="GeneID:10407"
                     /db_xref="HGNC:HGNC:14534"
                     /db_xref="MIM:606560"
                     /translation="MRQRLLPSVTSLLLVALLFPGSSQARHVNHSATEALGELRERAP
                     GQGTNGFQLLRHAVKRDLLPPRTPPYQGDVPPGIRNTICHMQQGICRLFFCHSGEKKR
                     DICSDPWNRCCVSNTDEEGKEKPEMDGRSGI"
     exon            101..253
                     /gene="SPAG11B"
                     /gene_synonym="EDDM2B; EP2; EP2C; EP2D; HE2; HE2C; SPAG11;
                     SPAG11A"
                     /inference="alignment:Splign:2.1.0"
     exon            254..551
                     /gene="SPAG11B"
                     /gene_synonym="EDDM2B; EP2; EP2C; EP2D; HE2; HE2C; SPAG11;
                     SPAG11A"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 accctgggtg ctttctggct tgcagtgctc ttggcagaca tgaggcaacg attgctcccg
       61 tccgtcacca gccttctcct tgtggccctg ctgtttccag gatcgtctca agccagacat
      121 gtgaaccact cagccactga ggctctcgga gaactcaggg aaagagcccc tgggcaaggc
      181 acaaacgggt ttcagctgct acgccacgca gtgaaacggg acctcttacc accgcgcacc
      241 ccaccttacc aaggggatgt tccaccggga attagaaata ccatctgcca tatgcagcaa
      301 gggatctgca gacttttttt ctgccattct ggtgagaaaa agcgtgacat ttgctctgat
      361 ccctggaata ggtgttgcgt atcaaataca gatgaagaag gaaaagagaa accagagatg
      421 gatggcagat ctgggatcta aaatataagc tcccggaagg cagggatgtt gaagtatccc
      481 aagggcttaa aggaatgtgt ggcttatagt aggtgttcaa taaatatttg ttgaatgaat
      541 ttagcaccaa a
//
