LOCUS       NM_003114               3695 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant
            1, mRNA.
ACCESSION   NM_003114
VERSION     NM_003114.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Liu,L., Liu,J., Liu,X.J., Liu,M.H., Feng,X.F., Yang,L. and Luo,J.M.
  TITLE     SPAG1 promotes the development of AML by activating the ERK/MAPK
            signaling pathway and affects the chemotherapy sensitivity of
            venetoclax
  JOURNAL   Neoplasma 69 (5), 1108-1118 (2022)
   PUBMED   35951456
  REMARK    GeneRIF: SPAG1 promotes the development of AML by activating the
            ERK/MAPK signaling pathway and affects the chemotherapy sensitivity
            of venetoclax.
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Smith,A.J., Bustamante-Marin,X.M., Yin,W., Sears,P.R.,
            Herring,L.E., Dicheva,N.N., Lopez-Giraldez,F., Mane,S., Tarran,R.,
            Leigh,M.W., Knowles,M.R., Zariwala,M.A. and Ostrowski,L.E.
  TITLE     The role of SPAG1 in the assembly of axonemal dyneins in human
            airway epithelia
  JOURNAL   J Cell Sci 135 (6) (2022)
   PUBMED   35178554
  REMARK    GeneRIF: The role of SPAG1 in the assembly of axonemal dyneins in
            human airway epithelia.
REFERENCE   3  (bases 1 to 3695)
  AUTHORS   Gu,Y., Chu,M.Q., Xu,Z.J., Yuan,Q., Zhang,T.J., Lin,J. and Zhou,J.D.
  TITLE     Comprehensive analysis of SPAG1 expression as a prognostic and
            predictive biomarker in acute myeloid leukemia by integrative
            bioinformatics and clinical validation
  JOURNAL   BMC Med Genomics 15 (1), 38 (2022)
   PUBMED   35227274
  REMARK    GeneRIF: Comprehensive analysis of SPAG1 expression as a prognostic
            and predictive biomarker in acute myeloid leukemia by integrative
            bioinformatics and clinical validation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3695)
  AUTHORS   Dermouche,S., Chagot,M.E., Manival,X. and Quinternet,M.
  TITLE     Optimizing the First TPR Domain of the Human SPAG1 Protein Provides
            Insight into the HSP70 and HSP90 Binding Properties
  JOURNAL   Biochemistry 60 (30), 2349-2363 (2021)
   PUBMED   33739091
  REMARK    GeneRIF: Optimizing the First TPR Domain of the Human SPAG1 Protein
            Provides Insight into the HSP70 and HSP90 Binding Properties.
REFERENCE   5  (bases 1 to 3695)
  AUTHORS   Shamsara,E. and Shamsara,J.
  TITLE     Bioinformatics analysis of the genes involved in the extension of
            prostate cancer to adjacent lymph nodes by supervised and
            unsupervised machine learning methods: The role of SPAG1 and
            PLEKHF2
  JOURNAL   Genomics 112 (6), 3871-3882 (2020)
   PUBMED   32619574
  REMARK    GeneRIF: Bioinformatics analysis of the genes involved in the
            extension of prostate cancer to adjacent lymph nodes by supervised
            and unsupervised machine learning methods: The role of SPAG1 and
            PLEKHF2.
REFERENCE   6  (bases 1 to 3695)
  AUTHORS   Ficarro,S., Chertihin,O., Westbrook,V.A., White,F., Jayes,F.,
            Kalab,P., Marto,J.A., Shabanowitz,J., Herr,J.C., Hunt,D.F. and
            Visconti,P.E.
  TITLE     Phosphoproteome analysis of capacitated human sperm. Evidence of
            tyrosine phosphorylation of a kinase-anchoring protein 3 and
            valosin-containing protein/p97 during capacitation
  JOURNAL   J Biol Chem 278 (13), 11579-11589 (2003)
   PUBMED   12509440
REFERENCE   7  (bases 1 to 3695)
  AUTHORS   Lin,W., Zhou,X., Zhang,M., Li,Y., Miao,S., Wang,L., Zong,S. and
            Koide,S.S.
  TITLE     Expression and function of the HSD-3.8 gene encoding a
            testis-specific protein
  JOURNAL   Mol Hum Reprod 7 (9), 811-818 (2001)
   PUBMED   11517287
REFERENCE   8  (bases 1 to 3695)
  AUTHORS   Diekman,A.B. and Herr,J.C.
  TITLE     Sperm antigens and their use in the development of an
            immunocontraceptive
  JOURNAL   Am J Reprod Immunol 37 (1), 111-117 (1997)
   PUBMED   9138444
  REMARK    Review article
REFERENCE   9  (bases 1 to 3695)
  AUTHORS   Zariwala,M.A., Knowles,M.R. and Leigh,M.W.
  TITLE     Primary Ciliary Dyskinesia
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301301
REFERENCE   10 (bases 1 to 3695)
  AUTHORS   Zhang,M.L., Wang,L.F., Miao,S.Y. and Koide,S.S.
  TITLE     Isolation and sequencing of the cDNA encoding the 75-kD human sperm
            protein related to infertility
  JOURNAL   Chin Med J (Engl) 105 (12), 998-1003 (1992)
   PUBMED   1299558
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC025647.9.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 19, 2019 this sequence version replaced NM_003114.4.
            
            Summary: The correlation of anti-sperm antibodies with cases of
            unexplained infertility implicates a role for these antibodies in
            blocking fertilization. Improved diagnosis and treatment of
            immunologic infertility, as well as identification of proteins for
            targeted contraception, are dependent on the identification and
            characterization of relevant sperm antigens. The protein expressed
            by this gene is recognized by anti-sperm agglutinating antibodies
            from an infertile woman. Furthermore, immunization of female rats
            with the recombinant human protein reduced fertility. This protein
            localizes to the plasma membrane of germ cells in the testis and to
            the post-acrosomal plasma membrane of mature spermatozoa.
            Recombinant polypeptide binds GTP and exhibits GTPase activity.
            Thus, this protein may regulate GTP signal transduction pathways
            involved in spermatogenesis and fertilization. Two transcript
            variants of this gene encode the same protein. [provided by RefSeq,
            Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF311312.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000388798.7/ ENSP00000373450.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AC025647.9         52273-52302
            31-172              AC025647.9         55965-56106
            173-332             AC025647.9         59500-59659
            333-458             AC025647.9         71502-71627
            459-520             AC025647.9         77061-77122
            521-627             AC025647.9         77642-77748
            628-733             AC025647.9         78314-78419
            734-864             AC025647.9         80806-80936
            865-971             AC025647.9         85076-85182
            972-1128            AC025647.9         87798-87954
            1129-1467           AC025647.9         106776-107114
            1468-1567           AC025647.9         107505-107604
            1568-1720           AC025647.9         113965-114117
            1721-1887           AC025647.9         118859-119025
            1888-2020           AC025647.9         124842-124974
            2021-2147           AC025647.9         127097-127223
            2148-2312           AC025647.9         132926-133090
            2313-2681           AC025647.9         134089-134457
            2682-3695           AC025647.9         134577-135590
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q22.2"
     gene            1..3695
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /note="sperm associated antigen 1"
                     /db_xref="GeneID:6674"
                     /db_xref="HGNC:HGNC:11212"
                     /db_xref="MIM:603395"
     exon            1..30
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            31..172
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     CDS             33..2813
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /note="TPR-containing protein involved in spermatogenesis;
                     tetratricopeptide repeat-containing protein;
                     infertility-related sperm protein Spag-1; epididymis
                     secretory protein Li 268; dynein axonemal assembly factor
                     13"
                     /codon_start=1
                     /product="sperm-associated antigen 1"
                     /protein_id="NP_003105.2"
                     /db_xref="CCDS:CCDS34930.1"
                     /db_xref="GeneID:6674"
                     /db_xref="HGNC:HGNC:11212"
                     /db_xref="MIM:603395"
                     /translation="MTTKDYPSLWGFGTTKTFKIPIEHLDFKYIEKCSDVKHLEKILC
                     VLRSGEEGYYPELTEFCEKHLQALAPESRALRKDKPAATAASFTAEEWEKIDGDIKSW
                     VSEIKKEEDKMHFHETETFPAMKDNLPPVRGSNSCLHVGKEKYSKRPTKKKTPRDYAE
                     WDKFDVEKECLKIDEDYKEKTVIDKSHLSKIETRIDTAGLTEKEKDFLATREKEKGNE
                     AFNSGDYEEAVMYYTRSISALPTVVAYNNRAQAEIKLQNWNSAFQDCEKVLELEPGNV
                     KALLRRATTYKHQNKLREATEDLSKVLDVEPDNDLAKKTLSEVERDLKNSEAASETQT
                     KGKRMVIQEIENSEDEEGKSGRKHEDGGGDKKPAEPAGAARAAQPCVMGNIQKKLTGK
                     AEGGKRPARGAPQRGQTPEAGADKRSPRRASAAAAAGGGATGHPGGGQGAENPAGLKS
                     QGNELFRSGQFAEAAGKYSAAIALLEPAGSEIADDLSILYSNRAACYLKEGNCSGCIQ
                     DCNRALELHPFSMKPLLRRAMAYETLEQYGKAYVDYKTVLQIDCGLQLANDSVNRLSR
                     ILMELDGPNWREKLSPIPAVPASVPLQAWHPAKEMISKQAGDSSSHRQQGITDEKTFK
                     ALKEEGNQCVNDKNYKDALSKYSECLKINNKECAIYTNRALCYLKLCQFEEAKQDCDQ
                     ALQLADGNVKAFYRRALAHKGLKNYQKSLIDLNKVILLDPSIIEAKMELEEVTRLLNL
                     KDKTAPFNKEKERRKIEIQEVNEGKEEPGRPAGEVSMGCLASEKGGKSSRSPEDPEKL
                     PIAKPNNAYEFGQIINALSTRKDKEACAHLLAITAPKDLPMFLSNKLEGDTFLLLIQS
                     LKNNLIEKDPSLVYQHLLYLSKAERFKMMLTLISKGQKELIEQLFEDLSDTPNNHFTL
                     EDIQALKRQYEL"
     exon            173..332
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            333..458
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            459..520
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            521..627
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            628..733
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            734..864
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            865..971
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1128
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1467
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1567
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1568..1720
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1721..1887
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1888..2020
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..2147
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            2148..2312
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            2313..2681
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     exon            2682..3695
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3695
                     /gene="SPAG1"
                     /gene_synonym="CILD28; CT140; DNAAF13; HEL-S-268; HSD-3.8;
                     SP75; TPIS"
                     /note="major polyA site"
ORIGIN      
        1 gttagtaggc ccgagcagtt tgaactggag ctatgaccac caaagattat ccatcattgt
       61 ggggctttgg aacaacaaaa acattcaaaa ttcccattga acatctagat ttcaaataca
      121 ttgaaaaatg ttcagatgtt aaacatctgg aaaaaattct ttgcgtgctc agatctggtg
      181 aggaaggata ttatcctgaa cttacagaat tttgtgaaaa gcatcttcaa gccttggccc
      241 ctgaaagcag agctttgagg aaagataaac cagcagcaac agcagccagt tttacagctg
      301 aagaatggga aaaaattgat ggtgatataa agagttgggt atcagaaatt aaaaaagaag
      361 aagataaaat gcactttcat gaaactgaga catttccagc aatgaaagat aatttgcctc
      421 cagttcgtgg ttcaaacagc tgtcttcatg taggcaagga aaaatattct aaaagaccaa
      481 ctaaaaagaa aactccaagg gattacgcgg aatgggataa atttgacgtg gagaaggaat
      541 gtttaaaaat tgatgaagat tacaaagaaa agacggtaat agacaagtca cacttgtcta
      601 aaattgagac aagaatagat acagcaggtc taactgagaa agaaaaggat tttcttgcca
      661 ctcgtgaaaa ggagaaagga aatgaagctt tcaactcagg agattatgaa gaagcagtga
      721 tgtattatac caggagcata tcagcgcttc ccactgtagt tgcctataac aatcgagctc
      781 aagcagaaat caaattacag aactggaata gtgcttttca ggattgtgaa aaggtcttgg
      841 agttagaacc tggaaacgta aaggctcttc tgcgtcgtgc tactacatat aaacatcaaa
      901 acaagctccg ggaagctaca gaagatttga gtaaagtact agatgttgag cctgataatg
      961 atttggccaa gaaaaccttg tcagaggttg aaagagatct gaaaaattct gaagctgcat
     1021 ctgagactca aaccaaaggg aaaaggatgg ttattcagga aatagaaaac tccgaagatg
     1081 aagaaggaaa aagcggaaga aaacatgaag atggcggtgg agataagaag cccgcggagc
     1141 cggcgggagc cgcgcgcgcc gcccagccgt gcgtcatggg caacatccag aagaagctga
     1201 ctggcaaagc cgaaggcggc aagcggccgg caaggggcgc gccgcagcgg ggccagaccc
     1261 cggaggccgg cgcggacaag cggagcccac ggcgggcctc tgcggcggcg gcggcgggcg
     1321 gcggcgccac cgggcatccg ggcggcgggc agggcgcgga gaaccctgcc ggcctgaaga
     1381 gccagggcaa cgagctgttc cgaagcgggc agttcgccga ggcggccggc aagtactcgg
     1441 cggcaatcgc gctcctggag ccagcaggaa gtgaaattgc agatgatcta agtatcttat
     1501 attcaaatag agcagcatgt tacctaaaag aaggaaactg cagtggctgc attcaagatt
     1561 gtaacagggc tctggaactt catccattct ctatgaaacc tcttctgagg cgggcgatgg
     1621 cctatgaaac tctagagcag tatgggaaag cttatgtgga ttataaaaca gtgttgcaga
     1681 tagactgtgg actccagcta gcaaatgaca gtgttaacag gctatcaaga attttaatgg
     1741 agctggatgg accaaattgg cgggagaagc tgtcacctat tcctgctgtg cctgcttctg
     1801 tgccactgca agcttggcat ccggcaaaag agatgatctc aaaacaagca ggagactcca
     1861 gcagccatcg ccagcagggc atcacagatg aaaaaacatt taaagccctt aaggaagaag
     1921 gaaatcaatg tgtaaatgac aaaaactata aagacgccct cagtaaatac agcgaatgct
     1981 taaagattaa caataaggaa tgtgccatat atacaaacag agctctctgt tacttgaagc
     2041 tgtgccagtt tgaagaagca aagcaggact gtgatcaggc acttcagcta gctgatggga
     2101 acgtgaaagc cttctataga cgagctctgg ctcataaagg actcaagaat tatcagaaaa
     2161 gcttaattga tctcaataaa gttatcctac tagatccaag tattattgag gcaaagatgg
     2221 aactggaaga ggtaactaga ctccttaatc ttaaggataa gacagcacca ttcaacaaag
     2281 aaaaggagag aaggaaaatt gagattcaag aggtgaatga aggcaaggag gagcctggaa
     2341 gacctgcagg ggaggtctcc atgggatgcc ttgcttctga gaagggaggc aaaagcagca
     2401 ggtcaccaga agaccctgag aaacttccga tagccaagcc taataatgcc tatgaatttg
     2461 gtcagattat aaatgctctc agtaccagga aggataaaga agcctgtgca catcttttag
     2521 ccatcactgc accaaaagat ttgccgatgt ttttaagtaa caaacttgaa ggggatacat
     2581 tccttctcct cattcagtct ctgaaaaata atcttattga aaaagatccc tcattggtgt
     2641 atcagcatct tttatacctg agtaaagcag aaaggtttaa gatgatgttg acactaatta
     2701 gcaagggcca aaaggagcta attgaacagc tgtttgagga cctttcggac acaccaaaca
     2761 accattttac tttagaagat atacaggccc taaaaaggca gtatgagctt taaatcaaga
     2821 taattgttag atttcttcca tgcatgtatg tgttccagga atgttaatga gatggtattg
     2881 taaaagagtt gcatggataa aacttggcct agaaaagttt ggtctgcact ataaaacatt
     2941 ttacttattt tcctacatag aacatgtata ttctacaatc tgctttttat tagttgtaaa
     3001 tattttctta tgtaccagaa ccaaataagt atatttagaa cttgttaaaa atacatttta
     3061 atttatgata tacatattat tttaattact tgttaaaatt ttgagttaag ttgcatttct
     3121 ttgggctatg aaggagtcct cttaagtttg atagaaatga atttcttgta acattctttt
     3181 ttaaaagtgg aagtcattaa cagtgattat tatatcactt atatcctgct aagatacaca
     3241 taaatcccat tttgtactag tacctgtgga ttacagtcag ttaaaatgaa atgcaacact
     3301 gaagtctata acatgaaatg attattaaat tgtttattaa tttagagcta taagaggaac
     3361 ttattttttc taatacggaa gcattgccta ataattaaga acaaaaattg ccaaaaattt
     3421 ctaccacttt ttactagatt ttaaaaagct actttctttt atattgccta tataagcaaa
     3481 aaaccaacca ctgtattaaa gcaaactaag cctgcattta tatctgaatt attacctcca
     3541 tattttacca aacatttgaa tgtccccctt cccccttttt tgttttctgc ttttatgact
     3601 gtatttattc ctttactgta aaagaatatg aagaactcat acatgttgaa agctcctttg
     3661 ttgactgggt caaagtgaag gaatttcaat ctcca
//
