LOCUS       NM_001316972            1239 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens sperm acrosome associated 6 (SPACA6), transcript
            variant 1, mRNA.
ACCESSION   NM_001316972
VERSION     NM_001316972.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Deneke,V.E., Blaha,A., Lu,Y., Suwita,J.P., Draper,J.M., Phan,C.S.,
            Panser,K., Schleiffer,A., Jacob,L., Humer,T., Stejskal,K.,
            Krssakova,G., Roitinger,E., Handler,D., Kamoshita,M., Vance,T.D.R.,
            Wang,X., Surm,J.M., Moran,Y., Lee,J.E., Ikawa,M. and Pauli,A.
  TITLE     A conserved fertilization complex bridges sperm and egg in
            vertebrates
  JOURNAL   Cell 187 (25), 7066-7078 (2024)
   PUBMED   39423812
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Vance,T.D.R., Yip,P., Jimenez,E., Li,S., Gawol,D., Byrnes,J.,
            Uson,I., Ziyyat,A. and Lee,J.E.
  TITLE     SPACA6 ectodomain structure reveals a conserved superfamily of
            gamete fusion-associated proteins
  JOURNAL   Commun Biol 5 (1), 984 (2022)
   PUBMED   36115925
  REMARK    GeneRIF: SPACA6 ectodomain structure reveals a conserved
            superfamily of gamete fusion-associated proteins.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1239)
  AUTHORS   Barbaux,S., Ialy-Radio,C., Chalbi,M., Dybal,E., Homps-Legrand,M.,
            Do Cruzeiro,M., Vaiman,D., Wolf,J.P. and Ziyyat,A.
  TITLE     Sperm SPACA6 protein is required for mammalian Sperm-Egg
            Adhesion/Fusion
  JOURNAL   Sci Rep 10 (1), 5335 (2020)
   PUBMED   32210282
  REMARK    GeneRIF: Sperm SPACA6 protein is required for mammalian Sperm-Egg
            Adhesion/Fusion.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1239)
  AUTHORS   Lorenzetti,D., Poirier,C., Zhao,M., Overbeek,P.A., Harrison,W. and
            Bishop,C.E.
  TITLE     A transgenic insertion on mouse chromosome 17 inactivates a novel
            immunoglobulin superfamily gene potentially involved in sperm-egg
            fusion
  JOURNAL   Mamm Genome 25 (3-4), 141-148 (2014)
   PUBMED   24275887
REFERENCE   5  (bases 1 to 1239)
  AUTHORS   Gascoigne,D.K., Cheetham,S.W., Cattenoz,P.B., Clark,M.B.,
            Amaral,P.P., Taft,R.J., Wilhelm,D., Dinger,M.E. and Mattick,J.S.
  TITLE     Pinstripe: a suite of programs for integrating transcriptomic and
            proteomic datasets identifies novel proteins and improves
            differentiation of protein-coding and non-coding genes
  JOURNAL   Bioinformatics 28 (23), 3042-3050 (2012)
   PUBMED   23044541
REFERENCE   6  (bases 1 to 1239)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC018755.3, BC041134.1 and KF751883.1.
            
            On May 20, 2020 this sequence version replaced NM_001316972.1.
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: KF751883.1, SRR5189667.56332.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000637797.2/ ENSP00000490829.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                AC018755.3         120479-120492
            15-291              BC041134.1         1-277
            292-803             KF751883.1         119-630
            804-804             AC018755.3         131212-131212
            805-1172            KF751883.1         632-999
            1173-1239           BC041134.1         1894-1960
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.41"
     gene            1..1239
                     /gene="SPACA6"
                     /gene_synonym="LET7EH; LINC00085; NCRNA00085; SPACA6P"
                     /note="sperm acrosome associated 6"
                     /db_xref="GeneID:147650"
                     /db_xref="HGNC:HGNC:27113"
                     /db_xref="MIM:618945"
     exon            1..411
                     /gene="SPACA6"
                     /gene_synonym="LET7EH; LINC00085; NCRNA00085; SPACA6P"
                     /inference="alignment:Splign:2.1.0"
     CDS             198..1172
                     /gene="SPACA6"
                     /gene_synonym="LET7EH; LINC00085; NCRNA00085; SPACA6P"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; long intergenic non-protein coding RNA 85;
                     BACHELOR-like protein; Let-7e host; sperm acrosome
                     associated 6, pseudogene; sperm acrosome
                     membrane-associated protein 6"
                     /codon_start=1
                     /product="sperm acrosome membrane-associated protein 6
                     isoform 1 precursor"
                     /protein_id="NP_001303901.1"
                     /db_xref="CCDS:CCDS82386.1"
                     /db_xref="GeneID:147650"
                     /db_xref="HGNC:HGNC:27113"
                     /db_xref="MIM:618945"
                     /translation="MALLALASAVPSALLALAVFRVPAWACLLCFTTYSERLRICQMF
                     VGMRSPKLEECEEAFTAAFQGLSDTEINYDERSHLHDTFTQMTHALQELAAAQGSFEV
                     AFPDAAEKMKKVITQLKEAQACIPPCGLQEFARRFLCSGCYSRVCDLPLDCPVQDVTV
                     TRGDQAMFSCIVNFQLPKEEITYSWKFAGGGLRTQDLSYFRDMPRAEGYLARIRPAQL
                     THRGTFSCVIKQDQRPLARLYFFLNVTGPPPRAETELQASFREVLRWAPRDAELIEPW
                     RPSLGELLARPEALTPSNLFLLAVLGALASASATVLAWMFFRWYCSGN"
     exon            412..489
                     /gene="SPACA6"
                     /gene_synonym="LET7EH; LINC00085; NCRNA00085; SPACA6P"
                     /inference="alignment:Splign:2.1.0"
     exon            490..558
                     /gene="SPACA6"
                     /gene_synonym="LET7EH; LINC00085; NCRNA00085; SPACA6P"
                     /inference="alignment:Splign:2.1.0"
     exon            559..582
                     /gene="SPACA6"
                     /gene_synonym="LET7EH; LINC00085; NCRNA00085; SPACA6P"
                     /inference="alignment:Splign:2.1.0"
     exon            583..660
                     /gene="SPACA6"
                     /gene_synonym="LET7EH; LINC00085; NCRNA00085; SPACA6P"
                     /inference="alignment:Splign:2.1.0"
     exon            661..770
                     /gene="SPACA6"
                     /gene_synonym="LET7EH; LINC00085; NCRNA00085; SPACA6P"
                     /inference="alignment:Splign:2.1.0"
     exon            771..927
                     /gene="SPACA6"
                     /gene_synonym="LET7EH; LINC00085; NCRNA00085; SPACA6P"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1138
                     /gene="SPACA6"
                     /gene_synonym="LET7EH; LINC00085; NCRNA00085; SPACA6P"
                     /inference="alignment:Splign:2.1.0"
     exon            1139..1239
                     /gene="SPACA6"
                     /gene_synonym="LET7EH; LINC00085; NCRNA00085; SPACA6P"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1216..1221
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPACA6"
                     /gene_synonym="LET7EH; LINC00085; NCRNA00085; SPACA6P"
                     /note="hexamer: AATAAA"
     polyA_site      1239
                     /gene="SPACA6"
                     /gene_synonym="LET7EH; LINC00085; NCRNA00085; SPACA6P"
                     /note="major polyA site"
ORIGIN      
        1 gcgtctggcc caaccacaca cctggggaat tgctggcctg acttctgacc cctgactcct
       61 catacccttc ctccagagca tgacatttga ccaccaactg aaacctgacc tctgacccca
      121 gaccactggc ccttcccccg ccctgtggtg acttcataaa ggttactagc ttctcccctg
      181 gccttgagac ccacacgatg gccctgctgg ctctggccag tgccgtcccg tctgccctgc
      241 tggccctggc tgtcttcagg gtgcccgcct gggcctgtct cctctgcttc acaacctact
      301 ctgagcgcct ccgcatctgc cagatgtttg ttgggatgcg gagccccaag cttgaagagt
      361 gtgaggaggc cttcacggcc gccttccagg gcctctctga caccgaaatc aactatgatg
      421 agagaagcca cctgcatgac accttcaccc agatgaccca tgccctgcag gagctggctg
      481 ctgcccaggg atcctttgag gttgccttcc ctgatgctgc ggagaaaatg aagaaggtca
      541 ttacacagct taaagaagcc caggcttgca tccctccctg cggtctccag gagttcgccc
      601 ggcgtttcct ctgcagcggg tgctactcta gggtctgcga cctcccgctg gactgcccag
      661 ttcaggatgt gacagtgact cggggcgacc aggctatgtt ttcttgcatc gtaaacttcc
      721 agctgccaaa ggaggagatc acctattcct ggaagttcgc aggaggaggt ctccggactc
      781 aggacttgtc ctatttccga gatatgccgc gggccgaagg atacctggcg cggatccggc
      841 cggctcagct cacgcaccgc gggacgttct cctgcgtgat caagcaagac cagcgccccc
      901 tggcccggct ctacttcttt cttaacgtga cgggcccgcc cccgcgggcg gagacagagt
      961 tgcaggcctc gttccgggaa gtgctgcgct gggcgccgcg ggatgccgag ctgatcgagc
     1021 cctggaggcc cagcctgggc gagctgctgg ccaggcccga ggctctgacg cccagcaatc
     1081 tgttcctgct tgcagtcctc ggggccctcg catcagcgag tgcgacagtg ttggcgtgga
     1141 tgttctttcg atggtactgc agtggcaact aacaaaggta tctttcctcc ttccctatcc
     1201 tatttccatc ctgaaaataa agaatatatt tcaactcta
//
