LOCUS       NM_133498                973 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens sperm acrosome associated 4 (SPACA4), mRNA.
ACCESSION   NM_133498
VERSION     NM_133498.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Chen,L., Song,J., Zhang,J., Luo,Z., Chen,X., Zhou,C. and Shen,X.
  TITLE     Spermatogenic cell-specific SPACA4 is essential for efficient
            sperm-zona pellucida binding in vitro
  JOURNAL   Front Cell Dev Biol 11, 1204017 (2023)
   PUBMED   37377732
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  AUTHORS   Herberg,S., Gert,K.R., Schleiffer,A. and Pauli,A.
  TITLE     The Ly6/uPAR protein Bouncer is necessary and sufficient for
            species-specific fertilization
  JOURNAL   Science 361 (6406), 1029-1033 (2018)
   PUBMED   30190407
REFERENCE   3  (bases 1 to 973)
  AUTHORS   Loughner,C.L., Bruford,E.A., McAndrews,M.S., Delp,E.E.,
            Swamynathan,S. and Swamynathan,S.K.
  TITLE     Organization, evolution and functions of the human and mouse
            Ly6/uPAR family genes
  JOURNAL   Hum Genomics 10, 10 (2016)
   PUBMED   27098205
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 973)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   5  (bases 1 to 973)
  AUTHORS   Shetty,J., Wolkowicz,M.J., Digilio,L.C., Klotz,K.L., Jayes,F.L.,
            Diekman,A.B., Westbrook,V.A., Farris,E.M., Hao,Z., Coonrod,S.A.,
            Flickinger,C.J. and Herr,J.C.
  TITLE     SAMP14, a novel, acrosomal membrane-associated,
            glycosylphosphatidylinositol-anchored member of the
            Ly-6/urokinase-type plasminogen activator receptor superfamily with
            a role in sperm-egg interaction
  JOURNAL   J Biol Chem 278 (33), 30506-30515 (2003)
   PUBMED   12788941
  REMARK    GeneRIF: SAMP14 represents a GPI-anchored putative receptor in the
            Ly-6/uPAR family that is exposed on the inner acrosomal membrane
            after the acrosome reaction.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC022154.4 and AF353721.2.
            
            On Nov 22, 2018 this sequence version replaced NM_133498.2.
            
            ##Evidence-Data-START##
            Transcript is intronless :: HM005480.1, AF353721.2 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000321762.3/ ENSP00000312774.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AC022154.4         8186-8186
            2-973               AF353721.2         1-972
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..973
                     /gene="SPACA4"
                     /gene_synonym="SAMP14"
                     /note="sperm acrosome associated 4"
                     /db_xref="GeneID:171169"
                     /db_xref="HGNC:HGNC:16441"
                     /db_xref="MIM:609932"
     exon            1..973
                     /gene="SPACA4"
                     /gene_synonym="SAMP14"
                     /inference="alignment:Splign:2.1.0"
     CDS             238..612
                     /gene="SPACA4"
                     /gene_synonym="SAMP14"
                     /note="sperm acrosomal membrane protein 14; sperm acrosome
                     membrane-associated protein 4; sperm acrosomal
                     membrane-associated protein 14; testicular tissue protein
                     Li 167"
                     /codon_start=1
                     /product="sperm acrosome membrane-associated protein 4
                     precursor"
                     /protein_id="NP_598005.1"
                     /db_xref="CCDS:CCDS12725.1"
                     /db_xref="GeneID:171169"
                     /db_xref="HGNC:HGNC:16441"
                     /db_xref="MIM:609932"
                     /translation="MVLCWLLLLVMALPPGTTGVKDCVFCELTDSMQCPGTYMHCGDD
                     EDCFTGHGVAPGTGPVINKGCLRATSCGLEEPVSYRGVTYSLTTNCCTGRLCNRAPSS
                     QTVGATTSLALGLGMLLPPRLL"
     regulatory      948..953
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SPACA4"
                     /gene_synonym="SAMP14"
                     /note="hexamer: AATAAA"
     polyA_site      973
                     /gene="SPACA4"
                     /gene_synonym="SAMP14"
                     /note="major polyA site"
ORIGIN      
        1 attccattca cagactcttg ttgggcagca gccacccgct cacctccatc cccaggactt
       61 agagggacgc agggcgttgg gaacagagga cactccaggc gctgaccctg ggaggccagg
      121 accagggcca aagtcccgtg ggcaagagga gtcctcagag gtccttcatt cagcggttcc
      181 gggaggtctg ggaagcccac ggcctggctg gggcagggtc aacgccgcca ggccgccatg
      241 gtcctgtgct ggctgctgct tctggtgatg gctctgcccc caggcacgac gggcgtcaag
      301 gactgcgtct tctgtgagct caccgactcc atgcagtgtc ctggtaccta catgcactgt
      361 ggcgatgacg aggactgctt cacaggccac ggggtcgccc cgggcactgg tccggtcatc
      421 aacaaaggct gcctgcgagc caccagctgc ggccttgagg aacccgtcag ctacaggggc
      481 gtcacctaca gcctcaccac caactgctgc accggccgcc tgtgtaacag agccccgagc
      541 agccagacag tgggggccac caccagcctg gcactggggc tgggtatgct gcttcctcca
      601 cgtttgctgt gaccaacagg gaggacaggg cctgggactg ttctcccaga tccgccactc
      661 cccatgtccc catgtccttc ccccactaaa tggccagaga ggccctggac aacctcttgc
      721 ggccctggct tcatcccttc taaggctgtc caccaggagc ccggtgctag gggaagcatc
      781 cccaggcctg actgagcggc aggggagcac ggcccgtggg tttgattgta ttactctgtt
      841 ccactggttc taagacgcag agcttctcac atctcaatca ggatgcttct ctccattggt
      901 agcactttag agtccatgaa atatggtaaa aaatatatat atatcataat aaatgacagc
      961 tgatgttcat gga
//
