LOCUS       NM_001080391            5414 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens SP100 nuclear antigen (SP100), transcript variant 1,
            mRNA.
ACCESSION   NM_001080391
VERSION     NM_001080391.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Gu,X., Zhuang,A., Yu,J., Yang,L., Ge,S., Ruan,J., Jia,R., Fan,X.
            and Chai,P.
  TITLE     Histone lactylation-boosted ALKBH3 potentiates tumor progression
            and diminished promyelocytic leukemia protein nuclear condensates
            by m1A demethylation of SP100A
  JOURNAL   Nucleic Acids Res 52 (5), 2273-2289 (2024)
   PUBMED   38118002
  REMARK    GeneRIF: Histone lactylation-boosted ALKBH3 potentiates tumor
            progression and diminished promyelocytic leukemia protein nuclear
            condensates by m1A demethylation of SP100A.
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Kim,D.K., Weller,B., Lin,C.W., Sheykhkarimli,D., Knapp,J.J.,
            Dugied,G., Zanzoni,A., Pons,C., Tofaute,M.J., Maseko,S.B.,
            Spirohn,K., Laval,F., Lambourne,L., Kishore,N., Rayhan,A.,
            Sauer,M., Young,V., Halder,H., la Rosa,N.M., Pogoutse,O.,
            Strobel,A., Schwehn,P., Li,R., Rothballer,S.T., Altmann,M.,
            Cassonnet,P., Cote,A.G., Vergara,L.E., Hazelwood,I., Liu,B.B.,
            Nguyen,M., Pandiarajan,R., Dohai,B., Coloma,P.A.R., Poirson,J.,
            Giuliana,P., Willems,L., Taipale,M., Jacob,Y., Hao,T., Hill,D.E.,
            Brun,C., Twizere,J.C., Krappmann,D., Heinig,M., Falter,C., Aloy,P.,
            Demeret,C., Vidal,M., Calderwood,M.A., Roth,F.P. and
            Falter-Braun,P.
  TITLE     A proteome-scale map of the SARS-CoV-2-human contactome
  JOURNAL   Nat Biotechnol 41 (1), 140-149 (2023)
   PUBMED   36217029
REFERENCE   3  (bases 1 to 5414)
  AUTHORS   Ma,Y., Li,J., Dong,H., Yang,Z., Zhou,L. and Xu,P.
  TITLE     PML Body Component Sp100A Restricts Wild-Type Herpes Simplex Virus
            1 Infection
  JOURNAL   J Virol 96 (8), e0027922 (2022)
   PUBMED   35353002
  REMARK    GeneRIF: PML Body Component Sp100A Restricts Wild-Type Herpes
            Simplex Virus 1 Infection.
REFERENCE   4  (bases 1 to 5414)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 5414)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 5414)
  AUTHORS   Sternsdorf,T., Jensen,K. and Will,H.
  TITLE     Evidence for covalent modification of the nuclear dot-associated
            proteins PML and Sp100 by PIC1/SUMO-1
  JOURNAL   J Cell Biol 139 (7), 1621-1634 (1997)
   PUBMED   9412458
REFERENCE   7  (bases 1 to 5414)
  AUTHORS   Sternsdorf,T., Jensen,K., Zuchner,D. and Will,H.
  TITLE     Cellular localization, expression, and structure of the nuclear dot
            protein 52
  JOURNAL   J Cell Biol 138 (2), 435-448 (1997)
   PUBMED   9230084
REFERENCE   8  (bases 1 to 5414)
  AUTHORS   Grotzinger,T., Jensen,K. and Will,H.
  TITLE     The interferon (IFN)-stimulated gene Sp100 promoter contains an
            IFN-gamma activation site and an imperfect IFN-stimulated response
            element which mediate type I IFN inducibility
  JOURNAL   J Biol Chem 271 (41), 25253-25260 (1996)
   PUBMED   8810287
REFERENCE   9  (bases 1 to 5414)
  AUTHORS   Dent,A.L., Yewdell,J., Puvion-Dutilleul,F., Koken,M.H., de The,H.
            and Staudt,L.M.
  TITLE     LYSP100-associated nuclear domains (LANDs): description of a new
            class of subnuclear structures and their relationship to PML
            nuclear bodies
  JOURNAL   Blood 88 (4), 1423-1426 (1996)
   PUBMED   8695863
REFERENCE   10 (bases 1 to 5414)
  AUTHORS   Szostecki,C., Guldner,H.H., Netter,H.J. and Will,H.
  TITLE     Isolation and characterization of cDNA encoding a human nuclear
            antigen predominantly recognized by autoantibodies from patients
            with primary biliary cirrhosis
  JOURNAL   J Immunol 145 (12), 4338-4347 (1990)
   PUBMED   2258622
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA562355.1, AF255565.1,
            AC010149.8 and AK160379.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001080391.1.
            
            Summary: This gene encodes a subnuclear organelle and major
            component of the PML (promyelocytic leukemia)-SP100 nuclear bodies.
            PML and SP100 are covalently modified by the SUMO-1 modifier, which
            is considered crucial to nuclear body interactions. The encoded
            protein binds heterochromatin proteins and is thought to play a
            role in tumorigenesis, immunity, and gene regulation. Alternatively
            spliced variants have been identified for this gene; one of which
            encodes a high-mobility group protein. [provided by RefSeq, Aug
            2011].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1, also known as SP100C).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF255565.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000340126.9/ ENSP00000343023.4
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                DA562355.1         46-110
            66-1352             AF255565.1         1-1287
            1353-1387           AC010149.8         27661-27695
            1388-2572           AF255565.1         1323-2507
            2573-2643           AC010149.8         102445-102515
            2644-3962           AC010149.8         103316-104634
            3963-4040           AK160379.1         3399-3476
            4041-4312           AC010149.8         104713-104984
            4313-4946           AK160379.1         3748-4381
            4947-4948           AC010149.8         105619-105620
            4949-5241           AK160379.1         4384-4676
            5242-5243           AC010149.8         105914-105915
            5244-5410           AK160379.1         4679-4845
            5411-5414           AC010149.8         106083-106086
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q37.1"
     gene            1..5414
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /note="SP100 nuclear antigen"
                     /db_xref="GeneID:6672"
                     /db_xref="HGNC:HGNC:11206"
                     /db_xref="MIM:604585"
     exon            1..128
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     CDS             97..2754
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /note="isoform 1 is encoded by transcript variant 1;
                     SP100-HMG nuclear autoantigen; nuclear dot-associated
                     Sp100 protein; speckled 100 kDa; nuclear autoantigen
                     Sp-100"
                     /codon_start=1
                     /product="nuclear autoantigen Sp-100 isoform 1"
                     /protein_id="NP_001073860.1"
                     /db_xref="CCDS:CCDS42832.1"
                     /db_xref="GeneID:6672"
                     /db_xref="HGNC:HGNC:11206"
                     /db_xref="MIM:604585"
                     /translation="MAGGGGDLSTRRLNECISPVANEMNHLPAHSHDLQRMFTEDQGV
                     DDRLLYDIVFKHFKRNKVEISNAIKKTFPFLEGLRDRDLITNKMFEDSQDSCRNLVPV
                     QRVVYNVLSELEKTFNLPVLEALFSDVNMQEYPDLIHIYKGFENVIHDKLPLQESEEE
                     EREERSGLQLSLEQGTGENSFRSLTWPPSGSPSHAGTTPPENGLSEHPCETEQINAKR
                     KDTTSDKDDSLGSQQTNEQCAQKAEPTESCEQIAVQVNNGDAGREMPCPLPCDEESPE
                     AELHNHGIQINSCSVRLVDIKKEKPFSNSKVECQAQARTHHNQASDIIVISSEDSEGS
                     TDVDEPLEVFISAPRSEPVINNDNPLESNDEKEGQEATCSRPQIVPEPMDFRKLSTFR
                     ESFKKRVIGQDHDFSESSEEEAPAEASSGALRSKHGEKAPMTSRSTSTWRIPSRKRRF
                     SSSDFSDLSNGEELQETCSSSLRRGSGSQPQEPENKKCSCVMCFPKGVPRSQEARTES
                     SQASDMMDTMDVENNSTLEKHSGKRRKKRRHRSKVNGLQRGRKKDRPRKHLTLNNKVQ
                     KKRWQQRGRKANTRPLKRRRKRGPRIPKDENINFKQSELPVTCGEVKGTLYKERFKQG
                     TSKKCIQSEDKKWFTPREFEIEGDRGASKNWKLSIRCGGYTLKVLMENKFLPEPPSTR
                     KKRILESHNNTLVDPCPENSNICEVCNKWGRLFCCDTCPRSFHEHCHIPSVEANKNPW
                     SCIFCRIKTIQERCPESQSGHQESEVLMRQMLPEEQLKCEFLLLKVYCDSKSCFFASE
                     PYYNREGSQGPQKPMWLNKVKTSLNEQMYTRVEGFVQDMRLIFHNHKEFYREDKFTRL
                     GIQVQDIFEKNFRNIFAIQETSKNIIMFI"
     exon            129..203
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            204..366
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            367..535
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            536..619
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            620..682
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            683..832
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            833..916
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1069
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1153
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1237
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1291
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1387
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1441
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..1525
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..1642
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1696
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1741
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            1742..1816
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            1817..1861
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            1862..1966
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            1967..2109
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            2110..2148
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            2149..2190
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            2191..2306
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            2307..2427
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            2428..2499
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            2500..2643
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     exon            2644..5414
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5390..5395
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /note="hexamer: ATTAAA"
     polyA_site      5414
                     /gene="SP100"
                     /gene_synonym="lysp100b"
                     /note="major polyA site"
ORIGIN      
        1 gcttggggcc tgggcagcca cactgcacgc aggctgggcc gactgagggg ctcagaggcc
       61 aggctctgag gcccacgcag ggcctagggt gggaagatgg caggtggggg cggcgacctg
      121 agcaccagga ggctgaatga atgtatttca ccagtagcaa atgagatgaa ccatcttcct
      181 gcacacagcc acgatttgca aaggatgttc acggaagacc agggtgtaga tgacaggctg
      241 ctctatgaca ttgtattcaa gcacttcaaa agaaataagg tggagatttc aaatgcaata
      301 aaaaagacat ttccattcct cgagggcctc cgtgatcgtg atctcatcac aaataaaatg
      361 tttgaagatt ctcaagattc ttgtagaaac ctggtccctg tacagagagt ggtgtacaat
      421 gttcttagtg aactggagaa gacatttaac ctgccagttc tggaagcact gttcagcgat
      481 gtcaacatgc aggaataccc cgatttaatt cacatttata aaggctttga aaatgtaatc
      541 catgacaaat tgcctctcca agaaagtgaa gaagaagaga gggaggagag gtctggcctc
      601 caactaagtc ttgaacaagg aactggtgaa aactcttttc gaagcctgac ttggccacct
      661 tcgggttccc catctcatgc tggtacaacc ccacctgaaa atggactctc agagcacccc
      721 tgtgaaacag aacagataaa tgcaaagaga aaagatacaa ccagtgacaa agatgattcg
      781 ctaggaagcc aacaaacaaa tgaacaatgt gctcaaaagg ctgagccaac agagtcctgc
      841 gaacaaattg ctgtccaagt gaataatggg gatgctggaa gggagatgcc ctgcccgttg
      901 ccctgtgatg aagaaagccc agaggcagag ctacacaacc atggaatcca aattaattcc
      961 tgttctgtgc gactggtgga tataaaaaag gaaaagccat tttctaattc aaaagttgag
     1021 tgccaagccc aagcaagaac tcatcataac caggcatctg acataatagt catcagcagt
     1081 gaggactctg aaggatccac tgacgttgat gagcccttag aagtcttcat ctcagcaccg
     1141 agaagtgagc ctgtgatcaa taatgacaac cctttagaat caaatgatga aaaggagggc
     1201 caagaagcca cttgctcacg accccagatt gtaccagagc ccatggattt cagaaaatta
     1261 tctacattca gagaaagttt taagaaaaga gtgataggac aagaccacga cttttcagaa
     1321 tccagtgagg aggaggcgcc cgcagaagcc tcgagcgggg cactgagaag caagcatggt
     1381 gagaaggctc ctatgacttc tagaagtaca tctacttgga gaatacccag caggaagaga
     1441 cgtttcagca gtagtgactt ttcagacctg agtaatggag aagagcttca ggaaacctgc
     1501 agctcatccc taagaagagg gtcaggatca cagccacaag aacctgaaaa taagaagtgc
     1561 tcctgtgtca tgtgttttcc aaaaggtgtg ccaagaagcc aagaagcaag gactgaaagt
     1621 agtcaagcat ctgacatgat ggataccatg gatgttgaaa acaattctac tttggaaaaa
     1681 cacagtggga aaagaagaaa aaagagaagg catagatcta aagtaaatgg tctccaaaga
     1741 gggagaaaga aagacagacc tagaaaacat ttaactctga ataacaaagt ccaaaagaaa
     1801 agatggcaac aaagaggaag aaaagccaac actagacctt tgaaaagaag aagaaaaaga
     1861 ggtccaagaa ttcccaaaga tgaaaatatt aattttaaac aatctgaact tcctgtgacc
     1921 tgtggtgagg tgaagggcac tctatataag gagcgattca aacaaggaac ctcaaagaag
     1981 tgtatacaga gtgaggataa aaagtggttc actcccaggg aatttgaaat tgaaggagac
     2041 cgcggagcat ccaagaactg gaagctaagt atacgctgcg gtggatatac cctgaaagtc
     2101 ctgatggaga acaaatttct gccagaacca ccaagcacaa gaaaaaagag aatactggaa
     2161 tctcacaaca ataccttagt tgacccttgt ccggaaaact caaatatatg tgaggtgtgc
     2221 aacaaatggg gacggctgtt ctgctgcgac acttgtccaa gatcctttca tgagcactgc
     2281 cacatcccat ccgtggaagc taacaagaac ccgtggagtt gcatcttctg caggataaag
     2341 actattcagg aaagatgccc agaaagccaa tcaggtcatc aggaatctga agtcctgatg
     2401 aggcagatgc tgcctgagga gcagttgaaa tgtgaattcc tcctcttgaa ggtctactgt
     2461 gattcgaaaa gctgcttttt cgcctcagaa ccgtattata acagagaggg gtctcagggc
     2521 ccacagaagc ccatgtggtt aaacaaagtc aagacaagtt tgaatgagca gatgtacacc
     2581 cgagtagaag ggtttgtgca ggacatgcgt ctcatctttc ataaccacaa ggaattttac
     2641 agggaagata aattcaccag actgggaatt caagtacagg acatctttga gaagaatttc
     2701 agaaacattt ttgcaattca ggaaacaagc aagaacatta taatgtttat ttagccattc
     2761 ttatctcctc ccttcagatc ctctggcagc tagctacgca atgtgcctgt ggtcccacta
     2821 atctgtgact gctcctgtgg aaactccaca tcacaattct ccaaaattta tcattgccat
     2881 tttaaaaccg tcttttcagc tttcaataaa attcaacacc ccttcatgtt aaaaattctc
     2941 aataagctag gtattgagga acatatccca aaataataag agccatttat gacaaaccca
     3001 cagacaacat tatatggaat gcgcaaaaga agcattcccc ttgaaaacaa gcacaagaca
     3061 aggattccct ctctcaccac tcctattcaa caaagtattg gaagtcctgg tcagagcagt
     3121 caggaagcag aaaaaaataa agggtatcta aataggcaaa gaggaagtca aactatccct
     3181 gtttgcacac aacattgatt ctatatctag aaaaccccct agtctcagcc cagaagctcc
     3241 ttctgctgat aaacaatttc agagatgttt cagaatacaa aattagtata tgaaaattac
     3301 tagtattcct atacaccagc aatagccaag ccaagagcca aatcaggaag gcaatctcat
     3361 tcacaattgc cactaaaaga ataaaatacc taggaataca gctaatcagg gaggtgagag
     3421 agttctacaa tgagaattac gaaacactgc tcaaagagat tggagatgac acaaacaaat
     3481 ggaaaaacat cccatgctcc tgtgtagaaa cagtcaatat cattaaaatg accatactgc
     3541 ccaaagcagt ttacaggttc aatgttattc ctatcaaacc accaatgaca ttcttcacag
     3601 aactagataa aactatttta aaattcatac agaaccaaaa aagagcccaa atagccaagg
     3661 caatcctaag caaaaagaac aaagctgaag gcatcacgtt accccacttc aaactatatt
     3721 acagggcttc agtaaccaaa acagcatggt actggtacca aaaaaaaagc cacatagacc
     3781 aatggaacag aacgaagagc acagaataag accacactcc tatgaccatc tgatcgtcga
     3841 taaaaacaag caatgggaaa aagactccct attttataaa tggtgctggg ataactggga
     3901 tagaagattg aagctagacc tcttccttac accatataca aaaatcaact caagatcaat
     3961 taaagactta atgtaaaatc aaaaactatg aagactctgg aagacaacct aggcaatacc
     4021 atcctggaca taggaacagg caaagatttc atgataaaga caaaagcaat agcaacaaaa
     4081 gcaaaatttg acaaatggga tctaattaaa cttaagagat tctgcacagc aaaagaaaca
     4141 atcaacagag taaacagaca acctacaaaa tgggagaaaa tatttgcaca ctatgcatct
     4201 gacaaaggtc taatagccag cttctatagg gaacttaaac aaatttacaa gacaaaaaga
     4261 aataacccca ttaaaaagtg ggcaaaggac atgaaagaca cttttttttt ttaagatgga
     4321 gtttcactct tgttgcccag gccagagtgc aatggcgtga tcttggctca ccacaacctc
     4381 tgcctcccgg gttcaagcaa ttctcctgcc tcagcctccc aggtggctgg gattacaggc
     4441 atgcaccacc tgactgattt tgtattttag tagagacggg gtttctccac attggtcagg
     4501 ctggtcttga actcccgacc tcaggtgatc cacccacctc ggcctcccaa agtgctggga
     4561 ttacaggcat cagccaccat gcccggatga aaagacactt tccaaaagaa gatacacatg
     4621 cggccaacaa gcatgtttta aaagctcaat atcactgatc gttagagaca tgcaaattaa
     4681 aactacaatg agacaccatc tcacaccagt caaaatgcct ctttctaaaa agtcaaaaaa
     4741 taacagctag taaggttgtg gagaaaaggg aacatttata cactattgat gggagtgtaa
     4801 attagttcaa ccactgtgga aagcagtgtg gcaactcctc atagtgctaa aagcagaact
     4861 gccattccac ccagcaatcc cattactggg tacataccca gaggaatata aatcattcta
     4921 ccataaagac acatgcatgc aaatgtccac tgcagcacta ttcacaatag caaagataca
     4981 gaatcaacct aagtgcccat cagtaacaga ttggataaag aaaatatggt acacatacac
     5041 catggaatag tatgcagcca taagaaacaa tgagatcatg tctcaggaac atggatagag
     5101 ctggaggcta ttatccttag caaactaatt caggaacaga aaaccaaata ccacaggttc
     5161 tcagttgtga gtgggagcta aatgatgaga actcatgaac acaatgaagg gaacagacac
     5221 tagggtctac ttgagggtgg aggatgggaa gagggagagg agcagaaaaa gtacctattg
     5281 gtgatgaagt actctgtaca acaaacccgt gacaagagtt tccctatata acaaaccttc
     5341 acatataccc ctgaacctaa aagttttttt aattgtaaat aaatggatca ttaaaaaaaa
     5401 ttttaataat aaaa
//
