LOCUS       NM_001395207            6774 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens sorbin and SH3 domain containing 2 (SORBS2),
            transcript variant 42, mRNA.
ACCESSION   NM_001395207
VERSION     NM_001395207.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Song,L., Zhao,X., Chen,J., Yin,H., Tang,H., Li,L., Dong,H., Li,X.,
            Qu,Z., Chu,X. and Guo,M.
  TITLE     RNA-binding protein SORBS2 increases human trophoblast cell
            migration via stabilizing HK2 mRNA in preeclampsia
  JOURNAL   Reproduction 168 (3), e240093 (2024)
   PUBMED   38995729
  REMARK    GeneRIF: RNA-binding protein SORBS2 increases human trophoblast
            cell migration via stabilizing HK2 mRNA in preeclampsia.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   Ban,Y., Yang,X., Tan,D., Gong,C., Gao,Y., Yuan,J., Chen,Y., Wang,Y.
            and Xu,T.
  TITLE     Sorbs2 regulates seizure activity by influencing AMPAR-mediated
            excitatory synaptic transmission in temporal lobe epilepsy
  JOURNAL   Neurochem Int 176, 105727 (2024)
   PUBMED   38555055
  REMARK    GeneRIF: Sorbs2 regulates seizure activity by influencing
            AMPAR-mediated excitatory synaptic transmission in temporal lobe
            epilepsy.
REFERENCE   3  (bases 1 to 6774)
  AUTHORS   Wang,B., Fan,X., Wang,L. and Wei,X.
  TITLE     The RNA-binding protein sorbin and SH3 domain-containing 2 are
            transcriptionally regulated by specificity protein 1 and function
            as tumor suppressors in bladder cancer by stabilizing tissue factor
            pathway inhibitor
  JOURNAL   Mol Carcinog 63 (6), 1174-1187 (2024)
   PUBMED   38501385
  REMARK    GeneRIF: The RNA-binding protein sorbin and SH3 domain-containing 2
            are transcriptionally regulated by specificity protein 1 and
            function as tumor suppressors in bladder cancer by stabilizing
            tissue factor pathway inhibitor.
REFERENCE   4  (bases 1 to 6774)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   5  (bases 1 to 6774)
  AUTHORS   Nakayama,M., Kikuno,R. and Ohara,O.
  TITLE     Protein-protein interactions between large proteins: two-hybrid
            screening using a functionally classified library composed of long
            cDNAs
  JOURNAL   Genome Res 12 (11), 1773-1784 (2002)
   PUBMED   12421765
REFERENCE   6  (bases 1 to 6774)
  AUTHORS   Xu,Y.C., Wu,R.F., Gu,Y., Yang,Y.S., Yang,M.C., Nwariaku,F.E. and
            Terada,L.S.
  TITLE     Involvement of TRAF4 in oxidative activation of c-Jun N-terminal
            kinase
  JOURNAL   J Biol Chem 277 (31), 28051-28057 (2002)
   PUBMED   12023963
REFERENCE   7  (bases 1 to 6774)
  AUTHORS   Kimura,A., Baumann,C.A., Chiang,S.H. and Saltiel,A.R.
  TITLE     The sorbin homology domain: a motif for the targeting of proteins
            to lipid rafts
  JOURNAL   Proc Natl Acad Sci U S A 98 (16), 9098-9103 (2001)
   PUBMED   11481476
REFERENCE   8  (bases 1 to 6774)
  AUTHORS   Zucconi,A., Dente,L., Santonico,E., Castagnoli,L. and Cesareni,G.
  TITLE     Selection of ligands by panning of domain libraries displayed on
            phage lambda reveals new potential partners of synaptojanin 1
  JOURNAL   J Mol Biol 307 (5), 1329-1339 (2001)
   PUBMED   11292345
REFERENCE   9  (bases 1 to 6774)
  AUTHORS   Kawabe,H., Hata,Y., Takeuchi,M., Ide,N., Mizoguchi,A. and Takai,Y.
  TITLE     nArgBP2, a novel neural member of ponsin/ArgBP2/vinexin family that
            interacts with synapse-associated protein 90/postsynaptic
            density-95-associated protein (SAPAP)
  JOURNAL   J Biol Chem 274 (43), 30914-30918 (1999)
   PUBMED   10521485
REFERENCE   10 (bases 1 to 6774)
  AUTHORS   Wang,B., Golemis,E.A. and Kruh,G.D.
  TITLE     ArgBP2, a multiple Src homology 3 domain-containing,
            Arg/Abl-interacting protein, is phosphorylated in v-Abl-transformed
            cells and localized in stress fibers and cardiocyte Z-disks
  JOURNAL   J Biol Chem 272 (28), 17542-17550 (1997)
   PUBMED   9211900
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC104805.3, AC108472.5 and
            AC093797.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: Arg and c-Abl represent the mammalian members of the
            Abelson family of non-receptor protein-tyrosine kinases. They
            interact with the Arg/Abl binding proteins via the SH3 domains
            present in the carboxy end of the latter group of proteins. This
            gene encodes the sorbin and SH3 domain containing 2 protein. It has
            three C-terminal SH3 domains and an N-terminal sorbin homology
            (SoHo) domain that interacts with lipid raft proteins. The
            subcellular localization of this protein in epithelial and cardiac
            muscle cells suggests that it functions as an adapter protein to
            assemble signaling complexes in stress fibers, and that it is a
            potential link between Abl family kinases and the actin
            cytoskeleton. Alternative splicing results in multiple transcript
            variants encoding different isoforms. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (42) represents the longest
            transcript and encodes the longest isoform (26).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000695409.1/ ENSP00000511888.1
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-531               AC104805.3         114788-115318       c
            532-671             AC104805.3         78351-78490         c
            672-727             AC104805.3         37223-37278         c
            728-777             AC108472.5         28312-28361         c
            778-870             AC108472.5         22504-22596         c
            871-897             AC108472.5         16546-16572         c
            898-1022            AC108472.5         16173-16297         c
            1023-1161           AC093797.3         167675-167813       c
            1162-1334           AC093797.3         162995-163167       c
            1335-1401           AC093797.3         158233-158299       c
            1402-1446           AC093797.3         157355-157399       c
            1447-1636           AC093797.3         155038-155227       c
            1637-1751           AC093797.3         152239-152353       c
            1752-1910           AC093797.3         144448-144606       c
            1911-1994           AC093797.3         143650-143733       c
            1995-2051           AC093797.3         140926-140982       c
            2052-2101           AC093797.3         136120-136169       c
            2102-2289           AC093797.3         132403-132590       c
            2290-3870           AC093797.3         128485-130065       c
            3871-3959           AC093797.3         125634-125722       c
            3960-4006           AC093797.3         124156-124202       c
            4007-4121           AC093797.3         120616-120730       c
            4122-4250           AC093797.3         120402-120530       c
            4251-4451           AC093797.3         117351-117551       c
            4452-4501           AC093797.3         99457-99506         c
            4502-4608           AC093797.3         95250-95356         c
            4609-6774           AC093797.3         91094-93259         c
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q35.1"
     gene            1..6774
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /note="sorbin and SH3 domain containing 2"
                     /db_xref="GeneID:8470"
                     /db_xref="HGNC:HGNC:24098"
                     /db_xref="MIM:616349"
     exon            1..531
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            532..671
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            672..727
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            728..777
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     CDS             768..4670
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /note="isoform 26 is encoded by transcript variant 42; Arg
                     binding protein 2; Arg/Abl-interacting protein 2;
                     arg-binding protein 2"
                     /codon_start=1
                     /product="sorbin and SH3 domain-containing protein 2
                     isoform 26"
                     /protein_id="NP_001382136.1"
                     /db_xref="CCDS:CCDS93679.1"
                     /db_xref="GeneID:8470"
                     /db_xref="HGNC:HGNC:24098"
                     /db_xref="MIM:616349"
                     /translation="MNTDSGGCARKRAAMSVTLTSVKRVQSSPNLLAAGRDSQSPDSA
                     WRSYNDGNQETLNGDATYSSLAAKGFRSVRPNLQDKRSPTQSQITVNGNSGGAVSPMS
                     YYQRPFSPSAYSLPASLNSSIVMQHGTSLDSTDTYPQHAQSLDGTTSSSIPLYRSSEE
                     EKRVTVIKAPHYPGIGPVDESGIPTAIRTTVDRPKDWYKTMFKQIHMVHKPDDDTDMY
                     NTPYTYNAGLYNPPYSAQSHPAAKTQTYRPLSKSHSDNSPNAFKDASSPVPPPHVPPP
                     VPPLRPRDRSSTEKHDWDPPDRKVDTRKFRSEPRSIFEYEPGKSSILQHERPPPLPTT
                     PTPVPREPGRKPLSSSRLGEVTGSPSPPPRSGAPTPSSRAPALSPTRTDRINPDDIDL
                     ENEPWYKFFSELEFGRPPPKKPLDYVQDHSSGVFNEASLYQSSIDRSLERPMSSASMA
                     SDFRKRRKSEPAVGPPRGLGDQSASRTSPGRVDLPGSSTTLTKSFTSSSPSSPSRAKG
                     GDDSKICPSLCSYSGLNGNPSSELDYCSTYRQHLDVPRDSPRAISFKNGWQMARQNAE
                     IWSSTEETVSPKIKSRSCDDLLNDDCDSFPDPKVKSESMGSLLCEEDSKESCPMAWGS
                     PYVPEVRSNGRSRIRHRSARNAPGFLKMYKKMHRINRKDLMNSEVICSVKSRILQYES
                     EQQHKDLLRAWSQCSTEEVPRDMVPTRISEFEKLIQKSKSMPNLGDDMLSPVTLEPPQ
                     NGLCPKRRFSIEYLLEEENQSGPPARGRRGCQSNALVPIHIEVTSDEQPRAHVEFSDS
                     DQDGVVSDHSDYIHLEGSSFCSESDFDHFSFTSSESFYGSSHHHHHHHHHHHRHLISS
                     CKGRCPASYTRFTTMLKHERARHENTEEPRRQEMDPGLSKLAFLVSPVPFRRKKNSAP
                     KKQTEKAKCKASVFEALDSALKDICDQIKAEKKRGSLPDNSILHRLISELLPDVPERN
                     SSLRALRRSPLHQPLHPLPPDGAIHCPPYQNDCGRMPRSASFQDVDTANSSCHHQDRG
                     GALQDRESPRSYSSTLTDMGRSAPRERRGTPEKEKLPAKAVYDFKAQTSKELSFKKGD
                     TVYILRKIDQNWYEGEHHGRVGIFPISYVEKLTPPEKAQPARPPPPAQPGEIGEAIAK
                     YNFNADTNVELSLRKGDRVILLKRVDQNWYEGKIPGTNRQGIFPVSYVEVVKKNTKGA
                     EDYPDPPIPHSYSSDRIHSLSSNKPQRPVFTHENIQGGGEPFQALYNYTPRNEDELEL
                     RESDVIDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL"
     exon            778..870
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            871..897
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            898..1022
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1161
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1334
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            1335..1401
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            1402..1446
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1636
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            1637..1751
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1910
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..1994
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            1995..2051
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            2052..2101
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            2102..2289
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            2290..3870
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            3871..3959
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            3960..4006
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            4007..4121
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            4122..4250
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            4251..4451
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            4452..4501
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            4502..4608
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     exon            4609..6774
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5467..5472
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /note="hexamer: ATTAAA"
     polyA_site      5494
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /note="major polyA site"
     regulatory      6752..6757
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /note="hexamer: AATAAA"
     polyA_site      6774
                     /gene="SORBS2"
                     /gene_synonym="ARGBP2; PRO0618"
                     /note="major polyA site"
ORIGIN      
        1 atattctgct gagcttcgcc ctggaagaag cctcttttat acatctcttc agggaagaga
       61 gaagcaatgg gcatgttagt atacaatgat cacagccacg caggcctgca agctgccttt
      121 tggacaggct gttgactgcc gttccaatta gctgattgga gaatgtggaa tgcagagtga
      181 taatgctgca tatctgctat caggcagcag caaaggtttt tgtcttggga aggcaagctt
      241 tccctgcaat attatctcag cagctcccta gctgcttacc ctgaaaacga gggatccaaa
      301 cggagggtgt tgcactctgc taacgctggt cctgtgcgtg gctgtggcat atgagcggca
      361 ggtctgaaaa agcaggtgtg tgctgggacg ggcactggac tggaacgcag gcggacgctc
      421 tcgggtttac ctgcttcctg ttaacagatt gtgggctccc agggcatatg tctgcacgct
      481 gaggccgagg cggagaaggg gcttcctgag cgtcccagta cactgacaga gacacttgga
      541 ttggacttaa tcttaaacct ctggagttca agacctttta aaaagggcta aataaacaat
      601 ctctacatgt aaaaggccac tgactcctac ttcctctgta tagagcaact gttgaactca
      661 gctgcctgta gagcgagaat aggacagact cgtataccat tctgatctgg gtgcatttct
      721 tcagaaggaa aactgaagac tttaataaca aactctccaa ggtgaaaatg aacacagata
      781 gcggtgggtg tgctcgcaaa cgtgccgcca tgtctgttac gctaacatct gtgaagagag
      841 tgcaaagttc tccaaaccta ttggctgcag ggcgtgattc tcagtcacca gactcagctt
      901 ggagatctta caatgatggc aatcaggaga cactgaacgg agatgctaca tattcctctc
      961 ttgcagcaaa aggttttaga agcgttcgac caaacctaca agataaaaga tcaccaactc
     1021 agagccagat aacagtgaat ggaaactcag gaggtgccgt gagtcccatg agttactatc
     1081 agaggccgtt ttccccctcg gcatattctc tcccagcctc actcaactcc agcattgtca
     1141 tgcagcacgg cacatccctc gattccacag acacatatcc ccagcatgcg cagtctctgg
     1201 atggcaccac cagcagctct atccccctgt accgatcctc agaggaagag aagagagtga
     1261 cagtcatcaa agccccgcat tacccaggga tcgggcccgt ggatgaatcc ggaatcccca
     1321 cagcaattag aacgacagtc gaccggccca aggactggta caagacgatg tttaagcaaa
     1381 ttcacatggt gcacaagccg gatgatgaca cagacatgta taatactcct tatacataca
     1441 atgcaggtct gtacaaccca ccctacagtg ctcagtcaca ccctgctgca aagacccaaa
     1501 cctacagacc tctttccaaa agccactccg acaacagccc caatgccttt aaggatgcgt
     1561 cctccccagt gcctccccca catgttccac ctccagtccc gccgcttcga ccaagagatc
     1621 ggtcttcaac agaaaagcat gactgggatc ctccagacag aaaagtggac acaagaaaat
     1681 ttcggtctga gccaaggagt atttttgaat atgaacctgg caagtcatca attcttcagc
     1741 atgaaagacc accccctctc ccaacaactc cgacacctgt tccaagggaa cctggccgga
     1801 agcctctttc tagctcccga ctcggagaag tcactggtag cccctcccct ccgccccgga
     1861 gtggcgcccc cacaccaagc tcgcgcgccc cggctctgtc tcccacccgg actgatcgca
     1921 taaatccaga tgacatagat ttagaaaatg agccctggta taaattcttt tcagaactgg
     1981 agtttggacg cccgcctcct aaaaagcctc tggactatgt tcaagatcat tcttctggtg
     2041 ttttcaatga ggcctccttg tatcagtcct ctatagacag aagcctggaa agacccatga
     2101 gttctgcaag catggccagt gacttcagga agcggaggaa gagcgagcct gcagtgggtc
     2161 caccacgggg cttgggagat caaagtgcga gcaggactag cccaggccga gtggacctcc
     2221 caggatcaag caccactctt acaaagtctt tcactagctc ttctccttct tccccatcaa
     2281 gagcaaaagg tggggatgat agcaaaatat gtccatccct ttgcagttac tcagggctca
     2341 acggcaaccc ctccagtgag ttagattact gtagcactta cagacagcac ttggatgtcc
     2401 ctcgggactc accaagagcc attagtttca agaacggctg gcaaatggcc cggcaaaacg
     2461 cagaaatctg gagcagcacg gaagaaaccg tctctcccaa aatcaaatcc cggagctgtg
     2521 acgatctcct aaacgacgac tgcgatagct tcccggaccc gaaagtcaag tcggaaagca
     2581 tgggctccct gttatgtgag gaggactcca aggagagctg ccccatggcg tgggggtccc
     2641 cctacgtccc ggaggttcgc agtaacggca gatcaaggat cagacacagg tcagcccgca
     2701 acgccccggg gttcctgaag atgtacaaga aaatgcaccg catcaaccgc aaggacctga
     2761 tgaactccga ggtgatctgc tccgtgaagt ccaggattct gcagtacgag agcgagcagc
     2821 agcacaagga cctgctgcgc gcctggagcc agtgctccac ggaggaggtg ccccgggaca
     2881 tggtgcccac acgcatctcc gaattcgaaa agctgatcca gaagtccaaa tccatgccaa
     2941 atttagggga tgacatgctg tctcccgtaa ccctagaacc accgcaaaat ggcctatgtc
     3001 ccaagaggcg gttttccatt gagtatttgc tggaggaaga aaatcaaagc ggcccccccg
     3061 ctcggggccg gcgaggctgc cagtctaacg ccctggtgcc cattcacatt gaagtcacca
     3121 gcgatgagca gccccgagca cacgtggagt tttccgacag cgaccaggac ggggttgtgt
     3181 ccgaccacag tgactacatt cacctagagg ggtcatcctt ctgcagtgaa agtgactttg
     3241 atcacttttc cttcacatcc tccgaaagct tttacggatc cagccaccac caccaccatc
     3301 accaccacca ccaccaccgc cacctcatca gctcctgcaa aggcaggtgc ccggcctcct
     3361 acactcgatt taccacaatg ttaaaacacg aaagagccag acacgaaaac accgaggagc
     3421 ccagaaggca agaaatggac cctggccttt ctaaacttgc ttttctagtc agtcctgtgc
     3481 ctttccggag gaaaaaaaat tcggctccta agaaacagac tgaaaaggca aaatgtaaag
     3541 catctgtgtt tgaggctctg gactctgccc ttaaagacat ctgtgaccaa attaaagctg
     3601 aaaagaaaag ggggagcttg ccggacaaca gcatcctgca ccgcctcatc agtgagctgc
     3661 tgccagatgt tcccgagagg aactcatccc tgagagcgct gaggaggagc cccctgcacc
     3721 agcctctcca cccactgcct cccgatggtg ctattcattg tccaccctac cagaatgact
     3781 gcgggagaat gccccgcagt gcctctttcc aagacgtgga cacagccaac agcagctgcc
     3841 accaccaaga ccgtggcggt gcactccaag accgtgagtc ccctagaagt tactcatcca
     3901 ctttgactga catggggaga agtgcaccaa gggaaagaag aggaactcca gaaaaagaga
     3961 aattgcctgc aaaagctgtt tatgatttta aggctcagac atctaaggag ttgtcattta
     4021 agaaaggaga tactgtctac atcctcagga aaattgatca aaattggtat gagggagaac
     4081 accacgggag agtgggcatc ttcccgatct catacgtaga gaaactcaca cctcctgaga
     4141 aagcacagcc tgcaagacca cctccgccag cccagcccgg agaaatcgga gaagctatag
     4201 ccaaatacaa cttcaacgca gacacaaatg tggagctgtc actgagaaag ggagatagag
     4261 ttattcttct taaaagagtt gatcaaaact ggtatgaagg taaaatccca ggaaccaaca
     4321 gacaaggcat cttccctgtt tcctatgtgg aggtcgtcaa gaagaacaca aaaggtgctg
     4381 aggactaccc tgaccctcca ataccccaca gctattctag tgataggatt cacagcttga
     4441 gctcaaataa gccacagcgt cctgtgttta ctcatgaaaa tattcaaggt gggggggaac
     4501 cgtttcaggc tctgtataac tatactccca ggaatgaaga tgagctggag ctcagagaaa
     4561 gtgatgtcat tgatgtcatg gaaaagtgtg atgacggctg gtttgtgggg acctcaagaa
     4621 gaaccaaatt ctttggtact ttccccggaa actacgtcaa gaggctgtga attgcgctcc
     4681 ctccttctgt agaggccgcc tgccagccat gcacctgcgt caacgcgcct gaaacacccc
     4741 gcgggcctcc cgttgtcatg ccttacggtt tccaatgcgc cgtcaccatc tccacctgcc
     4801 accaaaccac cagcagagta gccgccgctg ctgtgagcct ggggacgaca tggcaggctg
     4861 gtccccctcc gtgaaagtgt ggattcctac ttcctgctct aagctttgac acgtcaaaat
     4921 gtgggatcag aaagaaaaaa atcatgatat ttaaaaatgg tcaaatattt gaggcaaaaa
     4981 aaaaaaaaaa aagtgtctcc aggaggctgt ccagcctcgt ggctccattt caacatctcc
     5041 ccccaggcga tgttctcccc caagacgacc agaaaattgt ttattgggga atgctgtggt
     5101 ttgcattttc atattcttcg cttggcagtg tgtattcttt tcacaagttt gcctagtgtc
     5161 ttggtttaca caatatgaca actgtaactg tactttagct attgtttgcc tgcacataca
     5221 tgttgtaata tgcacagtga ttacaacctt taaagcaaga ggaggcgagt taatttggat
     5281 gagtgtgatt ttgctgattg aatgtgagtt ttcaaatagg agtctttttc tgcaatttgt
     5341 ttgcattttt tagaagtgca aacagtaagt aaataaaagc cttcggtaat aatcatgaca
     5401 atacaagagg ctgagctagg ctacagggga aaactattgt gttgtaaagt tgcatcgcta
     5461 ttttatatta aaatgtaatg atcagcatca tgaacaatga gctcttagtg ttttatttat
     5521 ctgaaaaaat gtaagtaaaa gcagtgttag tgagaggtgc aaagaattat tctaacagac
     5581 acctgaaagt atctgtaagc aatactagta ggtaagattt cactgtgtgt acacatacac
     5641 atttgagatt gtatgagaac atataatcca tatgatatgt tgtacatttt atggaaatgt
     5701 aatagaatct cacacattat tttatagaaa tagagtacag aagcatcaca agtattaaag
     5761 ttggttttag caaggattag gattactaca attattttta ctataataat tatttttctt
     5821 ctatggaact cagaatcctg gctacatttg aggacaggaa tatgttgagc ctgattttcc
     5881 tggtgtgtgt aaaatatttc ctaggaataa attaggcact ttttagaagc aatgttaaat
     5941 cattcaggtt ttattttctg ccctgaagca gaaatttaaa aaatgatatt gggacctgga
     6001 aggtttaata tggttcacag tgcctgaatt acacctgctc cgaaaactag ctttgtattt
     6061 cttatgactt tgcataagaa ctgttcatct ttggatcttg agcaccttac agataaaact
     6121 ttttatggca tctctttcat ggacagtgat attgatcttt tcacaatgta cgtagcctct
     6181 agaattttgt acatatgttt gctctttttt tgtacagact agttgttgag aaaacagggg
     6241 cgtttcctaa tttggtcttt atcctgcgac aacactttca gcagactagc ctctccttat
     6301 ctcacagatc acaagcaccc ctagatagtg tgattctgtc agatagcatt tatgcaaaaa
     6361 tctatgaagt taaaagatcg tagaagccaa atgaaatgta catatctact gactgatgac
     6421 aagggaattt cattaggaag aaggtaaaga aacatcgttg agtagcctac cttgatttct
     6481 gtcaagttca taaccagctt catattttaa aggcttcagg tttgaaatta agtcaactgc
     6541 atgcagcttt gctgataaat gaataattct ctttgatgcc atttatgaga aaagacttca
     6601 atatctgttg cctgtcatat ttaagaaaaa ttactgtttc tactctctgt atctgatttt
     6661 aaaagaaaaa actattcata cctggcttcc aggtaattga ctttgaattc ttacaagcaa
     6721 aggtcattgt gtttttcttg aatagacatc taataaattt tgcttggaag tata
//
