LOCUS       NM_000636              14167 bp    mRNA    linear   PRI 24-NOV-2024
DEFINITION  Homo sapiens superoxide dismutase 2 (SOD2), transcript variant 1,
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_000636
VERSION     NM_000636.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14167)
  AUTHORS   Azadmanesh,J., Slobodnik,K., Struble,L.R., Lutz,W.E., Coates,L.,
            Weiss,K.L., Myles,D.A.A., Kroll,T. and Borgstahl,G.E.O.
  TITLE     Revealing the atomic and electronic mechanism of human manganese
            superoxide dismutase product inhibition
  JOURNAL   Nat Commun 15 (1), 5973 (2024)
   PUBMED   39013847
  REMARK    GeneRIF: Revealing the atomic and electronic mechanism of human
            manganese superoxide dismutase product inhibition.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14167)
  AUTHORS   Shikholeslami,S.R. and Keshavarzi,F.
  TITLE     Investigation of the association between the CASP8rs1045485 and
            SOD2 rs4880 single nucleotide polymorphisms (SNPs) with breast
            cancer
  JOURNAL   Cancer Treat Res Commun 40, 100835 (2024)
   PUBMED   39038402
  REMARK    GeneRIF: Investigation of the association between the
            CASP8rs1045485 and SOD2 rs4880 single nucleotide polymorphisms
            (SNPs) with breast cancer.
REFERENCE   3  (bases 1 to 14167)
  AUTHORS   St Clair,D.K. and Holland,J.C.
  TITLE     Complementary DNA encoding human colon cancer manganese superoxide
            dismutase and the expression of its gene in human cells
  JOURNAL   Cancer Res 51 (3), 939-943 (1991)
   PUBMED   1988135
REFERENCE   4  (bases 1 to 14167)
  AUTHORS   Church,S.L.
  TITLE     Manganese superoxide dismutase: nucleotide and deduced amino acid
            sequence of a cDNA encoding a new human transcript
  JOURNAL   Biochim Biophys Acta 1087 (2), 250-252 (1990)
   PUBMED   1699607
  REMARK    Erratum:[Biochim Biophys Acta 1993 Jan 23;1171(3):341]
REFERENCE   5  (bases 1 to 14167)
  AUTHORS   Wispe,J.R., Clark,J.C., Burhans,M.S., Kropp,K.E., Korfhagen,T.R.
            and Whitsett,J.A.
  TITLE     Synthesis and processing of the precursor for human
            mangano-superoxide dismutase
  JOURNAL   Biochim Biophys Acta 994 (1), 30-36 (1989)
   PUBMED   2462451
REFERENCE   6  (bases 1 to 14167)
  AUTHORS   Heckl,K.
  TITLE     Isolation of cDNAs encoding human manganese superoxide dismutase
  JOURNAL   Nucleic Acids Res 16 (13), 6224 (1988)
   PUBMED   3399391
REFERENCE   7  (bases 1 to 14167)
  AUTHORS   Ho,Y.S. and Crapo,J.D.
  TITLE     Isolation and characterization of complementary DNAs encoding human
            manganese-containing superoxide dismutase
  JOURNAL   FEBS Lett 229 (2), 256-260 (1988)
   PUBMED   2831093
REFERENCE   8  (bases 1 to 14167)
  AUTHORS   Beck,Y., Oren,R., Amit,B., Levanon,A., Gorecki,M. and Hartman,J.R.
  TITLE     Human Mn superoxide dismutase cDNA sequence
  JOURNAL   Nucleic Acids Res 15 (21), 9076 (1987)
   PUBMED   3684581
REFERENCE   9  (bases 1 to 14167)
  AUTHORS   Barra,D., Schinina,M.E., Simmaco,M., Bannister,J.V.,
            Bannister,W.H., Rotilio,G. and Bossa,F.
  TITLE     The primary structure of human liver manganese superoxide dismutase
  JOURNAL   J Biol Chem 259 (20), 12595-12601 (1984)
   PUBMED   6386798
REFERENCE   10 (bases 1 to 14167)
  AUTHORS   Smith,M., Turner,B.M., Tanigaki,N. and Hirschhorn,K.
  TITLE     Regional localization of HLA, MES, and SODM on chromosome 6
  JOURNAL   Cytogenet Cell Genet 22 (1-6), 428-433 (1978)
   PUBMED   752518
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA318772.1, AK313082.1,
            X07834.1, AL135914.25 and AA126703.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_000636.3.
            
            Summary: This gene is a member of the iron/manganese superoxide
            dismutase family. It encodes a mitochondrial protein that forms a
            homotetramer and binds one manganese ion per subunit. This protein
            binds to the superoxide byproducts of oxidative phosphorylation and
            converts them to hydrogen peroxide and diatomic oxygen. Mutations
            in this gene have been associated with idiopathic cardiomyopathy
            (IDC), premature aging, sporadic motor neuron disease, and cancer.
            Alternative splicing of this gene results in multiple transcript
            variants. A related pseudogene has been identified on chromosome 1.
            [provided by RefSeq, Apr 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (A). Both variants 1 and
            2 encode the same isoform (A).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.33390.1,
                                           SRR1803614.215924.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000538183.7/
                                                  ENSP00000446252.1
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                DA318772.1         84-134
            52-443              AK313082.1         4-395
            444-1096            X07834.1           374-1026
            1097-4152           AL135914.25        50767-53822         c
            4153-4172           AA126703.1         24-43               c
            4173-14167          AL135914.25        40752-50746         c
FEATURES             Location/Qualifiers
     source          1..14167
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q25.3"
     gene            1..14167
                     /gene="SOD2"
                     /gene_synonym="GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1;
                     Mn-SOD; MNSOD; MVCD6"
                     /note="superoxide dismutase 2"
                     /db_xref="GeneID:6648"
                     /db_xref="HGNC:HGNC:11180"
                     /db_xref="MIM:147460"
     exon            1..97
                     /gene="SOD2"
                     /gene_synonym="GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1;
                     Mn-SOD; MNSOD; MVCD6"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..743
                     /gene="SOD2"
                     /gene_synonym="GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1;
                     Mn-SOD; MNSOD; MVCD6"
                     /EC_number="1.15.1.1"
                     /note="isoform A precursor is encoded by transcript
                     variant 1; mangano-superoxide dismutase; Mn superoxide
                     dismutase; indophenoloxidase B; manganese-containing
                     superoxide dismutase; superoxide dismutase 2,
                     mitochondrial; epididymis secretory sperm binding protein;
                     gastric cancer-associated lncRNA 1"
                     /codon_start=1
                     /product="superoxide dismutase [Mn], mitochondrial isoform
                     A precursor"
                     /protein_id="NP_000627.2"
                     /db_xref="CCDS:CCDS5265.1"
                     /db_xref="GeneID:6648"
                     /db_xref="HGNC:HGNC:11180"
                     /db_xref="MIM:147460"
                     /translation="MLSRAVCGTSRQLAPVLGYLGSRQKHSLPDLPYDYGALEPHINA
                     QIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGDVTAQIALQPALKFNGGGHINHSIFW
                     TNLSPNGGGEPKGELLEAIKRDFGSFDKFKEKLTAASVGVQGSGWGWLGFNKERGHLQ
                     IAACPNQDPLQGTTGLIPLLGIDVWEHAYYLQYKNVRPDYLKAIWNVINWENVTERYM
                     ACKK"
     exon            98..300
                     /gene="SOD2"
                     /gene_synonym="GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1;
                     Mn-SOD; MNSOD; MVCD6"
                     /inference="alignment:Splign:2.1.0"
     exon            301..417
                     /gene="SOD2"
                     /gene_synonym="GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1;
                     Mn-SOD; MNSOD; MVCD6"
                     /inference="alignment:Splign:2.1.0"
     exon            418..597
                     /gene="SOD2"
                     /gene_synonym="GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1;
                     Mn-SOD; MNSOD; MVCD6"
                     /inference="alignment:Splign:2.1.0"
     exon            598..14167
                     /gene="SOD2"
                     /gene_synonym="GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1;
                     Mn-SOD; MNSOD; MVCD6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      739..744
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SOD2"
                     /gene_synonym="GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1;
                     Mn-SOD; MNSOD; MVCD6"
                     /note="hexamer: AGTAAA"
     polyA_site      765
                     /gene="SOD2"
                     /gene_synonym="GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1;
                     Mn-SOD; MNSOD; MVCD6"
                     /note="major polyA site"
     regulatory      936..941
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SOD2"
                     /gene_synonym="GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1;
                     Mn-SOD; MNSOD; MVCD6"
                     /note="hexamer: AGTAAA"
     polyA_site      958
                     /gene="SOD2"
                     /gene_synonym="GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1;
                     Mn-SOD; MNSOD; MVCD6"
                     /note="major polyA site"
     regulatory      3670..3675
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SOD2"
                     /gene_synonym="GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1;
                     Mn-SOD; MNSOD; MVCD6"
                     /note="hexamer: ATTAAA"
     polyA_site      3697
                     /gene="SOD2"
                     /gene_synonym="GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1;
                     Mn-SOD; MNSOD; MVCD6"
     polyA_site      4172
                     /gene="SOD2"
                     /gene_synonym="GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1;
                     Mn-SOD; MNSOD; MVCD6"
     regulatory      14149..14154
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SOD2"
                     /gene_synonym="GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1;
                     Mn-SOD; MNSOD; MVCD6"
                     /note="hexamer: AATAAA"
     polyA_site      14167
                     /gene="SOD2"
                     /gene_synonym="GC1; GClnc1; IPO-B; IPOB; lncRNA-GC1;
                     Mn-SOD; MNSOD; MVCD6"
ORIGIN      
        1 actcgtggct gtggtggctt cggcagcggc ttcagcagat cggcggcatc agcggtagca
       61 ccagcactag cagcatgttg agccgggcag tgtgcggcac cagcaggcag ctggctccgg
      121 ttttggggta tctgggctcc aggcagaagc acagcctccc cgacctgccc tacgactacg
      181 gcgccctgga acctcacatc aacgcgcaga tcatgcagct gcaccacagc aagcaccacg
      241 cggcctacgt gaacaacctg aacgtcaccg aggagaagta ccaggaggcg ttggccaagg
      301 gagatgttac agcccagata gctcttcagc ctgcactgaa gttcaatggt ggtggtcata
      361 tcaatcatag cattttctgg acaaacctca gccctaacgg tggtggagaa cccaaagggg
      421 agttgctgga agccatcaaa cgtgactttg gttcctttga caagtttaag gagaagctga
      481 cggctgcatc tgttggtgtc caaggctcag gttggggttg gcttggtttc aataaggaac
      541 ggggacactt acaaattgct gcttgtccaa atcaggatcc actgcaagga acaacaggcc
      601 ttattccact gctggggatt gatgtgtggg agcacgctta ctaccttcag tataaaaatg
      661 tcaggcctga ttatctaaaa gctatttgga atgtaatcaa ctgggagaat gtaactgaaa
      721 gatacatggc ttgcaaaaag taaaccacga tcgttatgct gagtatgtta agctctttat
      781 gactgttttt gtagtggtat agagtactgc agaatacagt aagctgctct attgtagcat
      841 ttcttgatgt tgcttagtca cttatttcat aaacaactta atgttctgaa taatttctta
      901 ctaaacattt tgttattggg caagtgattg aaaatagtaa atgctttgtg tgattgaatc
      961 tgattggaca ttttcttcag agagctaaat tacaattgtc atttataaaa ccatcaaaaa
     1021 tattccatcc atatactttg gggacttgta gggatgcctt tctagtccta ttctattgca
     1081 gttatagaaa atctagtctt ttgccccagt tacttaaaaa taaaatatta acactttccc
     1141 aagggaaaca ctcggctttc tatagaaaat tgcacttttt gtcgagtaat cctctgcagt
     1201 gatacttctg gtagatgtca cccagtggtt tttgttaggt caaatgttcc tgtatagttt
     1261 ttgcaaatag agctgtatac tgtttaaatg tagcaggtga actgaactgg ggtttgctca
     1321 cctgcacagt aaaggcaaac ttcaacagca aaactgcaaa aaggtggttt ttgcagtagg
     1381 agaaaggagg atgtttattt gcagggcgcc aagcaaggag aattgggcag ctcatgcttg
     1441 agacccaatc tccatgatga cctacaagct agagtattta aaggcagtgg taaatttcag
     1501 gaaagcagaa gttaaaggca aaattgtaaa tcagtcgaga tcgggtgcct tcagggtggt
     1561 atggctgtat accaaaattg taaatcacta catgaagctt atatattggt ttggcctgaa
     1621 aggtgaagtg gggtaggcag ggggcgggct tacaggttat ggtggattca aagactccct
     1681 gatttgtgat tggttaagga agcaaagctt tgtctaaaaa cttggggtcc gcagaaagga
     1741 acattaaggt ctggccaggc ccctcaggaa gaaactgaga gcaaagaatg gaggtcagag
     1801 tttagtccct ggtgttcccc cttatctgac gtctgtgtga atccatttgg tgggggtctg
     1861 ggtttctgaa aagtagctca ggggcacgtg ttaaggatgt ctctaggtga ctctaacttc
     1921 cctggctatt gtttgaaact gttatgacct tcttgcttat cagcttgctg gtttccttct
     1981 cggggcgagc tgggtgcctg gagttttcgg tgaaggaaac tcaagattct cctttatttc
     2041 tgtgcttgtg ggaatccccc tggcacaccc caaagagggg tccctgctcc gtctcacagg
     2101 gatctttttg tatatttggc ttagcatcat acatttgcca tgttgtttca tcatctgcct
     2161 aatttactgt ttttgaatat ttcatttgtt tctaattgtt actacagata atgctggggt
     2221 gagcaactct gtgtacatag gtttatctcc tattggaata ttttctttat ataggcgttt
     2281 tttttttttc tttttttttg gagacagagt cttgctctgt tgcccaggct ggagtgcagt
     2341 ggcgcgaccg gagctcactg caacctccac ttcccgggtt caagtgattg tcccacctca
     2401 gcctcctgaa tagctgggat tacaggtgca tgctaccatg cctggctact ttttgtattt
     2461 ttagcagaga cagggtttca ccatgttggc cagggtggtc tcgaactcct gacctcaagt
     2521 gatccgtctg gctcagcctc ccaaagtgct gggattacag gtgtgagcca ctgcacctgg
     2581 cctatatagg cttttttctt aaacctattt agtaatgttt tcccaagttt attttttatt
     2641 tttaattttt tccccaagtt tatttttcta tttttttttc atggaaaaat ggggtaactt
     2701 agcagtttca atattgaaga ctgaagttta aaaaaaattt aaattcaagg tacttttaaa
     2761 attcagttag aaaagtaggc tttaaaaatt attagagaca agagtaccaa agcggtgtgt
     2821 gtatgtgtgt gtgtgtatgc atgcttgtgg attggaaaaa ctttggagac tgattacttt
     2881 tcattatata tgtgtcacag tgaaacagct tttatgtgtc atgtaagatt actgcttgcc
     2941 tctctaagga aggtcgtgac tgtttaaata gacgggcaag gtggaacctt ttgaaagatg
     3001 agcttttgaa tataagttgt ctgctagatc atggtttgta ttgaactaac aaggtttgca
     3061 gatctgctga cttatataaa gctttttgat tcctactaag ctttaagatt taaaaaatgt
     3121 tcaatgttga aatttctgtg gggctctatt tttgctttgg ctttctggtg agagagtgag
     3181 gaagcattct ttccttcact aagtttgtct ttcttgtctt ctggatagat tgattttaag
     3241 agactaaggg aatttacaaa ctaaagattt tagtcatctg gtggaaaagg agactttaag
     3301 attgtttagg gctgggcggg gtgactcaca tctgtaatcc cagcactttg ggaggccaag
     3361 gcaggcagaa cacttgaagg agttcaagac cagcgtggcc aacgtggtga aaccctgtct
     3421 ctactaaaaa tacaaaaatt gtttagctct gtttttcata atagaaatag aaaaggtaaa
     3481 attgcttttc ttctgaaaag aacaagtatt gttcatccaa gaagggtttt tgtgactgaa
     3541 tcagcagtgc ctgccctagt catagctgtg cttcaaaaac ctcagcatga ttagtgttgg
     3601 agcaaaacaa ggaagcaaag caaatactgt ttttgaaatt ctatctgttg cttgaactat
     3661 tttgtaataa ttaaactttg atgttgagaa atcacaactt tattgtacac ttcattgcaa
     3721 cttgaaattc atggtcttaa agtgagattt gaatttctat tgagcgcctt taaaaaagta
     3781 ataccaaacc ataaagttaa aatctatgta tattgagtca tatctaaaac cacgtataaa
     3841 cataaattgt atttcctgtt ttaattccag gggaagtact gtttgggaaa gctattatta
     3901 ggtaaatgtt ttacaaatta ctgtttctca ctttcagtca taccctaatg atcccagcaa
     3961 gataatgtcc tgtcttctaa gatgtgcatc aagcctggta catactgaaa accctataag
     4021 gtcctggata atttttgttt gattattcat tgaagaaaca tttattttcc aattgtgtga
     4081 agtttttgac tgttaataaa agaatctgtc aaccatcaaa gaggtctgca ttatgcttgc
     4141 atgtcaaaaa ctttaaaaat cctataatct tctgtcattt tcactgagtt tccatgggaa
     4201 aggaatagta aataatgggt agttgaaata ttactcttaa gaccaagacc tgtatctcca
     4261 gtcatatctg taataacatc atctgataac ctaaaagcat agtattaggg atatacgaca
     4321 aaaccaaagt gtttttgctg ttgtcacata ccactcaata cttttacacc aggttgtcca
     4381 gtggacacca gctggatgtc ctataattca attctgacac tgtctacctg gaattggagt
     4441 cagatcccac aggtggaggg ctcagcccac aagactgccc ccactccaga taccaatcgc
     4501 aagtccctat acttctgact ggctataaag tggggttccc gtgacccttt ccttggtttt
     4561 gatttatttg ctagagcagc tcccagaaca cagagaaaca ctctacttat gtttacccat
     4621 ttttaatttt attactttat taaaactaca tattggttac aatactaatt tattgtaaag
     4681 gatatttcat ttatttattt ttttatttag agatggagtc tcactgtgtc acccaggctg
     4741 gagtgcggtg gcacagtctc aactcactgc aacctccacc tcctgagttc aagcgattct
     4801 catgcctcag cctcccaagt agctgggatt acaagtgtgc accaccatgc ccagctaatt
     4861 tttgtatttt tagtagagat ggggtttcac tctgttggcc aggctggtct caaactcctg
     4921 acctcaagtc atccacccac ctcagcctcc caaagtgctg agattacagg tgtgagccat
     4981 tgcctctggc ttgtaaagga tatttcaaag aatacagatg aacagccaga tggaagagat
     5041 gcacagggca aggcatgtgg gaaggggtat ggagctttca tgctctctcc acgtcatcag
     5101 ctacccagaa gctcatctgg accccgaccc tttggggttt tacggaggct tcattatgtc
     5161 agcattacca ttggcaattg gcgattaact caaccttcag cctctctccc ctttctggag
     5221 gttgggggtt gaggctggga gtccaaaccc tccaatcttg ccttggtctt tctggtaagc
     5281 agccccccgt cctgaagcta cctagaggct cccagccacc aatcatttca ttggcaaacc
     5341 aaagatactt caaggggttt aggagccaag agaccaagca aggattgaga caatatatat
     5401 atttcttata aataacatga tcacaaatag tattttatat atttctcata atagctggtt
     5461 gtggttactc acacctatca tcccagctac taagaagcta aggcaggagg atcacaggcc
     5521 caggagtttg cagctgtagt gagctatgat tgtgctactg cactccagcc tgggtgacag
     5581 tgtgagaccc tgtctcttaa agttcttgta acatcttaga acattaacca accacagatt
     5641 attgccttaa ctctgaaaaa taaagtaatc atacttttac aagatgtatt ttacataaag
     5701 ttctggtgat attttactct ggtcattgga atataggagc ttaccacctg gtaccactct
     5761 tcctaaagtg gtatactatt tggtatagta ctggcatact atttggaaag ggatgtgaag
     5821 ttggcatgac ctgtctaggg tgaagtcatg ctgtctgcta ttcattgtgg gctttacagt
     5881 aatgagagtg cttctgttgt caagatgctt ctgggacacg ttaaaaaaaa tttaagattt
     5941 ctcagtttgt gaaaaggaag gagcacgaat gctttcaagt tcctatgaca tgctagtcat
     6001 ctttcagtgt gtcccattgt tgcacccagt agtctcgatg cccagcgtca cggtgattcc
     6061 aagtttgcct ctctgcccac tataatttgt gctccatgaa gtcccaggac atgaggttct
     6121 tgttcatttg tgcgtctggg cccatatcca aatattaaac aatgtgactt aatgtattta
     6181 tcatattgta aatatgggta tccccaaggc tcagaagtta acacagcata tccacagaga
     6241 ggtttgacag catggctgtg gtgactggga aggccttatg cttgctcctc cttctgctgc
     6301 ttgcctttgt gtgggcatgg aaagaacatt gaattaggta ctggaagacc tgggtcctgg
     6361 tcttggagat tttaccagag cacctgtggg atattggtca agatgcctgg ttttagtcct
     6421 gctcctttgc tgtgaaagag gatcttaagg tgccaactct ggcattttat tttttactta
     6481 tttattttta aattatactt taagttctag ggtacatgtg tacaacatgc aggtttgtta
     6541 catgtataca tgtgccatgt tggtgtgctg cacctattaa ctcgtcattt acattaggta
     6601 tatctcctaa tgctatccct cccccttctc cccacccctc gacaggcccc ggtgtgtgat
     6661 gttccccttc ctgtgtccaa gtgttctcat tgttcaattc ccatctatga gtgagaacat
     6721 gcggtgtttg tttttttgtc cttggaatag tttgctgaga aggatggttt ccagcttcat
     6781 ccatgtccct ataaaggaca tgaactcttc cttttttatg gctgcatagt attccatggt
     6841 gtatatgtgc cacatcttct taaaccaatc tatcattgat ggacatttgg gttggttcca
     6901 agtctttgct attgtgaaca gtgccacaat aaatatacgt gtgcatgtgt ctttatagca
     6961 gcatgattta taatcctttg ggtatatacc cagtaatggg atggctgggt caaatggtat
     7021 ttctagttct agatccctga ggaatcgcca cactgtcttc cacaatggtt gaactagttt
     7081 acagtcccac caccagtgta aaagtgttcc tatttctcca catcctctcc agcacctgtt
     7141 gtttcctgac tttttaatga ttgacattct aactggtgtg agatggtatc tcattgtggt
     7201 tttgatttgc atttctctga tggcccatga tgatgagcat ttttttcatg tgtctgttgg
     7261 atgcatggat gtcttctttt gagaagtgtc tcttcatatc cttcacctac tttttgatgg
     7321 ggtttgtttt tttcttgtaa gtttgtttga gttccttgta gattctggat attagccctt
     7381 tgtcagatga gtagattgca aaaattttct cccattttgt aggttgcctg ttcactctga
     7441 tggtagtttc ttttgctgtg cagaagctct tgagtttaat gagatcccat ttgtcaattt
     7501 tggcttctgt tgccattgct tttggtgttt tagacatgaa gtccttgccc atgcctgtgt
     7561 cctgaatggt actgcatagg ttttcttcta gggtttttat ggttttaggt ctaacattta
     7621 agtctttaat ccatcttgaa ttaatttttg tataaggtgt aaggaaggga tccagtttca
     7681 gctttctaca tgtggctagc cagttttccc agcaccattt attaaatagg gaatcctttc
     7741 cccatttctt gtttttgtca ggtttctcaa agatcagata gttgtagatg tgtggtatta
     7801 tttctgaggg ctctgttctg ttccattggt ctatatctct gttttggtac tagtaccatg
     7861 ctgttttggt tactgcagcc ttgtagtata gtttgaaatc aggtagcgtg atgcctccag
     7921 ctttgttctt ttggcttagg atcgttttgg cagtgcaggc tcttttttgg ttccacatga
     7981 actttaaagt agttttttcc aattctatga agaaagtcat gggtagcttg atggggatgg
     8041 cattgaatct ataaattacc ttgggcagta tagccatttt cacgatattg attcttccta
     8101 tccatgagca tggaatgttc ttccatttgt ttgtgtcctc ttttatttcc ttgagcagtg
     8161 gtttgtagtt ctccttgaag aggtccttca catcccttgt aagttggatt cctaggtatt
     8221 ttattctctt tgaggcagtt gtgaatggga gttcactcat gatttggctc tctgtttgtc
     8281 tgttgttggt gtataagaat gcttgtgatt tttgcacatt gattttgtat cctgagattt
     8341 tgctgaagtt gcttatcaaa ttaaggagat tttgggctga gatgatgggg ttttctaaat
     8401 ataaaatcat gtcatctgca aacagggaca atttgacttc ctcttttcct tattgaatac
     8461 cctttatttc tttctcctgc ctgatggccc tggccagaac ttccaacact atgttgaata
     8521 ggagtggtga gagagggcat ccctgtcttg tgccagtttt caaagggaat gcttccagtt
     8581 tttgcccatt cagtctgata ttggctgtgg gtttgtcata gatagctctt attattttga
     8641 gatatgtccc atcaatacct aattcattga gagtttttag catgaagggc tgttgaattt
     8701 tgtccaaggc cttttctgca tctgttgaga taatcatgtg gtttttgtct ttggttctgt
     8761 ttatatgctg gattactttt attgatttgt gtatgttgaa ccagccttgc atcccaggga
     8821 tgaagcccac ttgatcatgg tggataagct ttttgatatg ctgctggatt cagtttgcca
     8881 gtattttatt gaggattttt gcattgattt tcatcaggga tattggtcta aagttctctt
     8941 tttttgttat gtctctgcca ggctttggta tcaggatgat gctggcttca taaaatgagt
     9001 tagggaggat tccctctttt tctattgatt ggaatagttt cagaaggaat ggtaccagct
     9061 cttccttgta cctctggtag aattcggctg tgaatccatc ttgtcctgga ctttttttgg
     9121 ttggtaggct attaattatt gcctcaattt cagagcctgt tattggtcta ttcagggatt
     9181 aaacttcttc ctggtttagt tttgagaggg tgtatgtgtc caggaattta tctatttctt
     9241 ctagattttc tagtttattt gcatagaggt gtttatagta ttctctgtgt actctgtatt
     9301 tctgtgggat tggtggtgat atccacttta tcatttttta ttgcatttat ttgattcttc
     9361 tctcttttct tgtttattag tcttgctagc ggtctatcaa ttgtgtttat cttttcaaaa
     9421 aaccagctcc tggattcatt cattttttga aggttttttt gtgtctctgt ctccttcagt
     9481 tctgctctga tcttagttat ttcttgtctt ctgctagctg ttgaatgtgt ttgctcttgc
     9541 ttctctaata tttttaattg tgatgttaga gtgtcaattt tagatctttc ctgctttctc
     9601 ttgtgggcac ttagtgctgt aaatttccct ctacacactg ctttaaatgt gtcccagaga
     9661 ttctggtatg ttgtgtcttt gttctcgttg gtttcaaaga acatctttat ttctgccttc
     9721 attttgttat gtacccagta gtcattcagg agcaggttgt tcagtttcca tgtagctgag
     9781 tggttttgag tgagtttctt aatcctgagt tctagtttga ttgcactgtg gtctgagaca
     9841 gtatgttata atttctgttc ttttacattt gctgaggagt gctttacttc caactatgtg
     9901 gtcaattttg gaataggtgc gatgtggtgc tgagaagaat gtatattctg ttgatttggg
     9961 gtggagagtt ctgtagatgt ctattaggtc tgcttggtgc agagctgaat tcaattcctg
    10021 gatatccttg ttaactttct gtctcgttga tctgtctaat attgacagtg gggtgttaga
    10081 gtctcccatt gttattgtgt gggagtctaa atctctttgt aggtctctaa ggacttggtt
    10141 tatgaatctg ggtgctcctg tattgggtgc atatatattt aagatagtta gctcttcttg
    10201 ttgaattgat ccctttacta tcatgtaatg gccttctttg tctcttttga tctttgttgg
    10261 tttaaagtct gttttatcag agactaggat tgcaaccccg gccttttttt ttgttttccg
    10321 tttgcttggt agatcttcct ccatcctttt attttgagcc tatgtgtgtg tgtctctgta
    10381 cgtgagatgg gtttcctgaa tacagcaccc tgatgggtct tgactcttta tccagtgtgc
    10441 cagtctgtgt cttttaattg gagcatttag cccatttaca tttaaggtta atattgttat
    10501 gtgtgaattt gatcctgtca ctaggatgtt cactggttat tttgctcgtt agttgatgca
    10561 gtttcttcct agcctcaatg gtctttacaa tttggcatgt ttttgcagtg gctgatactg
    10621 gttgttccat tccatgttta gtgcttcctt caggagctct tgtaaggcag gcctggtggt
    10681 gacaaaatct ctcagcattt gcttgtttgt aaaggatttt atttctcctt cacttatgaa
    10741 gcttagtttg gctggatatg aaattctggg ttgaaaattc ttttcttcaa gaacgttgaa
    10801 tattgacccc cactctcttc tggcttgtag agtttctgct gagagatcag ctgttagtct
    10861 gatgggcttc cctttgtggg taacccgacc tttctctctg gctgccctta acattttttc
    10921 cttcatttca actttggtga atctgacaat tatgtgtctt ggagttgctc ttctcgagga
    10981 gtatctttgt ggcgttctct gtatttcctg aatttgaatg ttgtcctccc ttgctagatt
    11041 ggggaagttc tcctggataa tatcctgcag agtgttttcc agcttggttc cattctcccc
    11101 gtcactttca ggtacaccaa tcagacgtag atttggtctt ttcacatagt cccatatttc
    11161 ttggaagctt tgttcgtttc tttttactct tttttctctt aacttctctt ctcacttcat
    11221 ttcattcatc tgatcttcaa tcactgataa cctttcttcc agttgatcga attggctact
    11281 gaagcttgtg cattcgtcac gtagttctca tgccatggtt ttcagctcca tcaggtcatt
    11341 taaggacttc tctatgctgg ttattctagt tagccatttg tctaatcttt tttcaaggtt
    11401 tttaggttct ttgtgatggg ttcgaacttc ctcttttagc ttggagaagt ttgatcatct
    11461 gaagccttct tctctcaact cgtcaaagtc attctccgtc cagctttgtt ctgttgctgg
    11521 caaggagcta tgttcctttg gagggggaga ggcgctctga tttttagaat tttcagcttt
    11581 tctgctctgt tttttcccca tctttgtggt tttatctacc tttggtcttt ggtgatggtg
    11641 atgtgcagat ggggttttgg tgtggatgtc ctttctgttt gttagttttc cttctaacag
    11701 tcaggaccgc cagctgcagg tctgttggag tttgctggac gtccacgcca gaccctgttt
    11761 gcctgggtat cacagtggag gctgtagaac agcgaatatt gctgaacagc aaatgttgct
    11821 gcctgatcat tcctctggaa gcttcatctc agaggggtac ccggccatgt ggggtgtcag
    11881 tctgccccta cttgggggtg cctcccagtt aggctactcg ggggtcaggg actcacttga
    11941 ggaggcagtc tctccgttct cagatctcaa actctgtgct gggagaacca ctggtctctt
    12001 caaagctgtc agacagggac attgaagtct gcagagattt ctgctgcctt ttgttcggct
    12061 atgccatgcc cccagaggtg gagtctacag aggcaggcag gcaggcctcc ttgagctgca
    12121 gtgggctcca cctagttcga gcttcccggc tgctttattt acctactgaa gcctcagcaa
    12181 tggtgggctc ccctccccta gcctcactgc caccttgcag ttcgatctca gactgctgtg
    12241 ctagcaatga gcaaggctcc atgggcgtgg gaccctccga gctaggcaca ggatataatc
    12301 tcctggtgtg ccgtttgcta agaccattgg aaaaacgcag tattagggtg ggagtgaccc
    12361 aattttccag gtgccgtctg tcacagcttc ccttggctag gaaagggaat tccctgaccc
    12421 cttgccttcc cgggtgaggc catgccttgc cctgcttcag ctcatgctca gtgggctgca
    12481 cccaccgtcc tacacccact gtccaacaag ccccagtgag atgaacctgg tacctcagtt
    12541 ggaagtgcag aaatcacctg tcttctgcgt cgctcacgct gggagctgta gactggagct
    12601 gttcctattc ggccatcttg gaacccaacc caagtctggc attttttggt tttctctgtg
    12661 tattgactat gattatagag gataaatgga tattgatatt attaaatcgt catttacaga
    12721 aggcacacaa cttatatcct ttaaacaaag ttctccaacc agtagggtat acttgctggt
    12781 agtgcttaca gctgccacca ttcttcactg gggctcagag tggatgcctg gaggaaaaaa
    12841 acaaatgaat cttttagctc ctagttactg ttagcatttt taccagaaaa caatttaaaa
    12901 aatgacacaa gtaagtcttc acctatcaag gctgagtgtg gtggcctata cttgtaatct
    12961 ctgtgctttg ggaggccaac gcaggaggat ctcttgagtc caggagtttg agaccagcct
    13021 gggcaacaaa gcaagacccc atctctacag aaagttaaaa tttagctggg catgctggca
    13081 catccctata gtcccagcta cttgagaggc tgagttggga ggatcccttg aggtcatgag
    13141 ttggagtcta cagtgagctg tgattacagt gtgagtctac actgcactcc agtctgggtg
    13201 aaagagatcc tgtctctcaa aaaacaagtg attctcctgg gaagagattt gtttaaaaag
    13261 tctgcacctc tattgatgat aactttgaat gtaaatggat taaattctcc cacttaaaag
    13321 atatagattg actgaatgga ttaaaaacca ccatcatgac tcgactgtat gctgcctaca
    13381 agaaacctca cctgtaaaga caggccaaaa gtgaaggtat ggagaaagat attgcataca
    13441 aatggaagcc aaaaaagagc agaaataact atacttacat gagataaaat agactttaag
    13501 tcaaaaactg taaaaagaaa agtcattatg taatgaaaca aggatcaatt catgcaagat
    13561 ataatcatat ttttttatta agaaaatttt tttgtagaga tggggttatg ctatgttgcc
    13621 tggctagtct caaactctgg gtctcaaagc agtcctcctg ctttggcctc ctaaggtgct
    13681 gggattatag gcatgagcca ctgtgccagg cctgaggata ttatcattat aaatatatat
    13741 gtaccccaca ctggatgacc cagatataaa gcaaatatta ttagatttaa agggagaaat
    13801 agactctaat acaatagtag ttgaggactt cactctactc tcagcattgg acagattttc
    13861 tagacataaa accaataaat attggattta aattgcactt tacaccaaat ggacctgaca
    13921 tttacagaac attctaatag gtgcagaaca ctctaatagc tgcagaacac acgttctttt
    13981 catcagcaca tggaacattc accagactag ttctggaacc cacttcctgc cttagtgttc
    14041 tttccaccac agtgcatgat aattccgaca gaacgccttt tatttgtacc ttattagttg
    14101 tagggggaaa gctttttcat gtacaaaaat gctgtaattt tctgatttaa taaagtaatt
    14161 ccactga
//
