LOCUS       NM_003101               6840 bp    mRNA    linear   PRI 01-AUG-2024
DEFINITION  Homo sapiens sterol O-acyltransferase 1 (SOAT1), transcript variant
            1, mRNA.
ACCESSION   NM_003101
VERSION     NM_003101.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Hong,Y., Abudukeremu,X., She,F. and Chen,Y.
  TITLE     SOAT1 in gallbladder cancer: Clinicopathological significance and
            avasimibe therapeutics
  JOURNAL   J Biochem Mol Toxicol 38 (6), e23733 (2024)
   PUBMED   38770938
  REMARK    GeneRIF: SOAT1 in gallbladder cancer: Clinicopathological
            significance and avasimibe therapeutics.
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Fu,R., Xue,W., Liang,J., Li,X., Zheng,J., Wang,L., Zhang,M. and
            Meng,J.
  TITLE     SOAT1 regulates cholesterol metabolism to induce EMT in
            hepatocellular carcinoma
  JOURNAL   Cell Death Dis 15 (5), 325 (2024)
   PUBMED   38724499
  REMARK    GeneRIF: SOAT1 regulates cholesterol metabolism to induce EMT in
            hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6840)
  AUTHORS   Farhat,N.Y., Alexander,D., McKee,K., Iben,J., Rodriguez-Gil,J.L.,
            Wassif,C.A., Cawley,N.X., Balch,W.E. and Porter,F.D.
  TITLE     Sterol O-Acyltransferase 1 (SOAT1): A Genetic Modifier of
            Niemann-Pick Disease, Type C1
  JOURNAL   Int J Mol Sci 25 (8), 4217 (2024)
   PUBMED   38673803
  REMARK    GeneRIF: Sterol O-Acyltransferase 1 (SOAT1): A Genetic Modifier of
            Niemann-Pick Disease, Type C1.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6840)
  AUTHORS   Wang,Z., Wang,M., Zhang,M., Xu,K., Zhang,X., Xie,Y., Zhang,Y.,
            Chang,C., Li,X., Sun,A. and He,F.
  TITLE     High-affinity SOAT1 ligands remodeled cholesterol metabolism
            program to inhibit tumor growth
  JOURNAL   BMC Med 20 (1), 292 (2022)
   PUBMED   35941608
  REMARK    GeneRIF: High-affinity SOAT1 ligands remodeled cholesterol
            metabolism program to inhibit tumor growth.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6840)
  AUTHORS   Cases,S., Novak,S., Zheng,Y.W., Myers,H.M., Lear,S.R., Sande,E.,
            Welch,C.B., Lusis,A.J., Spencer,T.A., Krause,B.R., Erickson,S.K.
            and Farese,R.V. Jr.
  TITLE     ACAT-2, a second mammalian acyl-CoA:cholesterol acyltransferase.
            Its cloning, expression, and characterization
  JOURNAL   J Biol Chem 273 (41), 26755-26764 (1998)
   PUBMED   9756919
REFERENCE   6  (bases 1 to 6840)
  AUTHORS   Yang,H., Cromley,D., Wang,H., Billheimer,J.T. and Sturley,S.L.
  TITLE     Functional expression of a cDNA to human acyl-coenzyme
            A:cholesterol acyltransferase in yeast. Species-dependent substrate
            specificity and inhibitor sensitivity
  JOURNAL   J Biol Chem 272 (7), 3980-3985 (1997)
   PUBMED   9020103
REFERENCE   7  (bases 1 to 6840)
  AUTHORS   Chang,C.C., Noll,W.W., Nutile-McMenemy,N., Lindsay,E.A.,
            Baldini,A., Chang,W. and Chang,T.Y.
  TITLE     Localization of acyl coenzyme A:cholesterol acyltransferase gene to
            human chromosome 1q25
  JOURNAL   Somat Cell Mol Genet 20 (1), 71-74 (1994)
   PUBMED   8197480
REFERENCE   8  (bases 1 to 6840)
  AUTHORS   Chang,C.C., Huh,H.Y., Cadigan,K.M. and Chang,T.Y.
  TITLE     Molecular cloning and functional expression of human acyl-coenzyme
            A:cholesterol acyltransferase cDNA in mutant Chinese hamster ovary
            cells
  JOURNAL   J Biol Chem 268 (28), 20747-20755 (1993)
   PUBMED   8407899
REFERENCE   9  (bases 1 to 6840)
  AUTHORS   Cadigan,K.M., Chang,C.C. and Chang,T.Y.
  TITLE     Isolation of Chinese hamster ovary cell lines expressing human
            acyl-coenzyme A/cholesterol acyltransferase activity
  JOURNAL   J Cell Biol 108 (6), 2201-2210 (1989)
   PUBMED   2738092
REFERENCE   10 (bases 1 to 6840)
  AUTHORS   Cadigan,K.M., Heider,J.G. and Chang,T.Y.
  TITLE     Isolation and characterization of Chinese hamster ovary cell
            mutants deficient in acyl-coenzyme A:cholesterol acyltransferase
            activity
  JOURNAL   J Biol Chem 263 (1), 274-282 (1988)
   PUBMED   3335499
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC344271.1, BC028940.1,
            AL833625.1, AL451075.15 and BQ182606.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_003101.5.
            
            Summary: The protein encoded by this gene belongs to the
            acyltransferase family. It is located in the endoplasmic reticulum,
            and catalyzes the formation of fatty acid-cholesterol esters. This
            gene has been implicated in the formation of beta-amyloid and
            atherosclerotic plaques by controlling the equilibrium between free
            cholesterol and cytoplasmic cholesteryl esters. Alternatively
            spliced transcript variants have been found for this gene.
            [provided by RefSeq, Nov 2011].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript, and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.204046.1,
                                           SRR1660807.233547.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            MANE Ensembl match                 :: ENST00000367619.8/
                                                  ENSP00000356591.3
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-85                DC344271.1         84-168
            86-2002             BC028940.1         1-1917
            2003-2955           AL833625.1         1932-2884
            2956-3478           BC028940.1         2871-3393
            3479-6754           AL451075.15        38602-41877
            6755-6840           BQ182606.1         16-101              c
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q25.2"
     gene            1..6840
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /note="sterol O-acyltransferase 1"
                     /db_xref="GeneID:6646"
                     /db_xref="HGNC:HGNC:11177"
                     /db_xref="MIM:102642"
     exon            1..140
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /inference="alignment:Splign:2.1.0"
     exon            141..266
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             149..1801
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /EC_number="2.3.1.26"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sterol O-acyltransferase (acyl-Coenzyme A: cholesterol
                     acyltransferase) 1; acyl-coenzyme A:cholesterol
                     acyltransferase 1"
                     /codon_start=1
                     /product="sterol O-acyltransferase 1 isoform 1"
                     /protein_id="NP_003092.4"
                     /db_xref="CCDS:CCDS1330.1"
                     /db_xref="GeneID:6646"
                     /db_xref="HGNC:HGNC:11177"
                     /db_xref="MIM:102642"
                     /translation="MVGEEKMSLRNRLSKSRENPEEDEDQRNPAKESLETPSNGRIDI
                     KQLIAKKIKLTAEAEELKPFFMKEVGSHFDDFVTNLIEKSASLDNGGCALTTFSVLEG
                     EKNNHRAKDLRAPPEQGKIFIARRSLLDELLEVDHIRTIYHMFIALLILFILSTLVVD
                     YIDEGRLVLEFSLLSYAFGKFPTVVWTWWIMFLSTFSVPYFLFQHWATGYSKSSHPLI
                     RSLFHGFLFMIFQIGVLGFGPTYVVLAYTLPPASRFIIIFEQIRFVMKAHSFVRENVP
                     RVLNSAKEKSSTVPIPTVNQYLYFLFAPTLIYRDSYPRNPTVRWGYVAMKFAQVFGCF
                     FYVYYIFERLCAPLFRNIKQEPFSARVLVLCVFNSILPGVLILFLTFFAFLHCWLNAF
                     AEMLRFGDRMFYKDWWNSTSYSNYYRTWNVVVHDWLYYYAYKDFLWFFSKRFKSAAML
                     AVFAVSAVVHEYALAVCLSFFYPVLFVLFMFFGMAFNFIVNDSRKKPIWNVLMWTSLF
                     LGNGVLLCFYSQEWYARQHCPLKNPTFLDYVRPRSWTCRYVF"
     exon            267..325
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /inference="alignment:Splign:2.1.0"
     exon            326..477
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /inference="alignment:Splign:2.1.0"
     exon            478..537
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /inference="alignment:Splign:2.1.0"
     exon            538..645
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /inference="alignment:Splign:2.1.0"
     exon            646..928
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /inference="alignment:Splign:2.1.0"
     exon            929..1007
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1089
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1090..1135
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1265
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1266..1363
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1462
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1598
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1599..1744
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1745..6840
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2714..2719
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /note="hexamer: AATAAA"
     polyA_site      2736
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /note="major polyA site"
     regulatory      6817..6822
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /note="hexamer: ATTAAA"
     polyA_site      6840
                     /gene="SOAT1"
                     /gene_synonym="ACACT; ACAT; ACAT-1; ACAT1; SOAT; STAT"
                     /note="major polyA site"
ORIGIN      
        1 gctttagctt agcaggcgac gttgcgggcc ctgggcgcca ggagagcttc ccggagtcga
       61 ccttcctgct ggctgctctg tgaccgcttc ccggctctgc cctcttggcc gaagtgcccg
      121 ctgccgggcg cgggcctcag acaatacaat ggtgggtgaa gagaagatgt ctctaagaaa
      181 ccggctgtca aagtccaggg aaaatcctga ggaagatgaa gaccagagaa accctgcaaa
      241 ggagtcccta gagacaccta gtaatggtcg aattgacata aaacagttga tagcaaagaa
      301 gataaagttg acagcagagg cagaggaatt gaagccattt tttatgaagg aagttggcag
      361 tcactttgat gattttgtga ccaatctcat tgaaaagtca gcatcattag ataatggtgg
      421 gtgcgctctc acaacctttt ctgttcttga aggagagaaa aacaaccata gagcgaagga
      481 tttgagagca cctccagaac aaggaaagat ttttattgca aggcgctctc tcttagatga
      541 actgcttgaa gtggaccaca tcagaacaat atatcacatg tttattgccc tcctcattct
      601 ctttatcctc agcacacttg tagtagatta cattgatgaa ggaaggctgg tgcttgagtt
      661 cagcctcctg tcttatgctt ttggcaaatt tcctaccgtt gtttggacct ggtggatcat
      721 gttcctgtct acattttcag ttccctattt tctgtttcaa cattgggcca ctggctatag
      781 caagagttct catccgctga tccgttctct cttccatggc tttcttttca tgatcttcca
      841 gattggagtt ctaggttttg gaccaacata tgttgtgtta gcatatacac tgccaccagc
      901 ttcccggttc atcattatat tcgagcagat tcgttttgta atgaaggccc actcatttgt
      961 cagagagaac gtgcctcggg tactaaattc agctaaggag aaatcaagca ctgttccaat
     1021 acctacagtc aaccagtatt tgtacttctt atttgctcct acccttatct accgtgacag
     1081 ctatcccagg aatcccactg taagatgggg ttatgtcgct atgaagtttg cacaggtctt
     1141 tggttgcttt ttctatgtgt actacatctt tgaaaggctt tgtgccccct tgtttcggaa
     1201 tatcaaacag gagcccttca gcgctcgtgt tctggtccta tgtgtattta actccatctt
     1261 gccaggtgtg ctgattctct tccttacttt ttttgccttt ttgcactgct ggctcaatgc
     1321 ctttgctgag atgttacgct ttggtgacag gatgttctat aaggattggt ggaactccac
     1381 gtcatactcc aactattata gaacctggaa tgtggtggtc catgactggc tatattacta
     1441 tgcttacaag gactttctct ggtttttctc caagagattc aaatctgctg ccatgttagc
     1501 tgtctttgct gtatctgctg tagtacacga atatgccttg gctgtttgct tgagcttttt
     1561 ctatcccgtg ctcttcgtgc tcttcatgtt ctttggaatg gctttcaact tcattgtcaa
     1621 tgatagtcgg aaaaagccga tttggaatgt tctgatgtgg acttctcttt tcttgggcaa
     1681 tggagtctta ctctgctttt attctcaaga atggtatgca cgtcagcact gtcctctgaa
     1741 aaatcccaca tttttggatt atgtccggcc acgttcctgg acttgtcgtt acgtgtttta
     1801 gaagcttgga ctttgtttcc tccttgtcac tgaagattgg gtagctccct gatttggagc
     1861 cagctgtttc cagttgttac tgaagttatc tgtgttattt ggaccactcc aggctttaca
     1921 gatgactcac tccattccta ggtcacttga agccaaactg ttggaagttc actggagtct
     1981 tgtacactta agcagagcag aacttttttt gtggggctgg gtggggggag aagaccgact
     2041 aacagctgaa gtaatgacag attgttgctg ggtcatatca gctttatccc ttggtaatta
     2101 tatctgtttt gtttcttgac tctgtccaat cagagaataa acatcatagt ttcttggcca
     2161 ctgaattagc caaaacactt aggaagaaat cacttaaata cctctggctt agaaattttt
     2221 tcatgcacac tgttggaatg tatgctaatt gaacatgcaa ttggggaaga aaaaatgtag
     2281 aatgattttt gctatttcta gtagaaagaa aatgtctgtt ttccaaagat aatgttatac
     2341 atcctatttt gtaatttttt tgaaaaaagt tcaatgttca gttttcctta gtttttacct
     2401 tgttttctct ataggtcatg atttctgtga agcaaaaaga tgccttttac catgaattct
     2461 tgagtttaca tcaataatat tgtatattaa ggggatcaga agtaggaagg aaaaaataag
     2521 agatagcaga ggaaaaagaa aaacatttcc tcttataact tctgaagtaa tttgtaaaaa
     2581 agatttgtag agtcaatcat gtgtttaaat tattttatca caaacttaac atggaagata
     2641 ttccttttta actttgtggt aacttctttg aagttattta gaaatatcct ttggaacaat
     2701 tattttattg tctaataaat attgacttct cttgaattat tttgcagact agtgagtctg
     2761 taccataagt attaatcacc tccactcata ttaaagtgat cattaagaat ccagaagctg
     2821 gcttctgcat ttgctcagtt atacttttaa tggtagtatg tttttaggtg gaataaatta
     2881 atatgtgatt ggtttcaagg aaatgtactc tattatgtaa tacttccatt ttataagatg
     2941 cccatttcta atacaatgtg tgtaggaatt atttgtatgt atgaggtatg attgtaaaga
     3001 ttgagcattg gaaggggtat cagagaccat gtagttcaac tttccactca aagtaagatt
     3061 tatgaattat ttaaatgata gttgttactt ggaacagcca cttgagaggc tctcttggta
     3121 ttgtttggaa ttgttttaaa gtcagtttga gtctcacaag aaggtaagta gagtttctta
     3181 atagtcacaa cttaatttga accacaagta tccagcttaa tcacgtatct tactcacgat
     3241 accactggtc cagatgagtt taggtaattt tcaaacatta aatccaactt caacggaaac
     3301 aaatacactc aagggaagtt atttttaaaa aggtactctg catgttcccg cagtaatgtt
     3361 ctgcacaaca gtattgtaat tgtaatggaa tcataacctg ctaactagtt tgctttaata
     3421 tggcttgtaa ttcttgacat ttttcttaaa attaaaacga atttttattt tgaatttata
     3481 actgcatttt aaatatattg gggacagatt gcgctgagac ctggttatga gcaagccaat
     3541 cttttgaatc tagagaatgg aattcttagg tttatatttc tgttaagaaa tactataaat
     3601 atgactctta tgagaagact ttgttgctct gtagtgtttc tgaatactgt atttgttgga
     3661 ttgatcaagg ctatttttca aaaagctctc tgcttcctgt ttgtttgttt gtttgttttt
     3721 gagacagagt cttgctctgt cgccggggct ggagtgcaat ggcgtgaact cagctcactg
     3781 caacctctgc ctccctggtt caagctattc tcctgcctca gcctcctgag tagctgggat
     3841 tacagggcca cgcctggcta atttttgtat ttttagtaga gatggggttt caccctgttg
     3901 gtcaggctgg tctcaaactc ctgaccttgt gatctgccca cctcagcctc ccaaagtgct
     3961 gggaagacag gcgttagcca ccgtgcccgg cctctgtttc ctgttattag tgattttcct
     4021 gcccaagatt gcaacaacaa atatgtagaa ctacagactg tttagaatgc tgagactgtt
     4081 ctaagaaact ttcaaaaaca gtagcacttc aaggaatggt cactttctat gaaagaaact
     4141 ggtttgatag ccataatctt attgctagct gcttttagca aaagtctttt cttgaaacca
     4201 ccacctatac tctttaaaca aataaaaact aaaatctctt gctaatgttt caggaagatt
     4261 tattttttct attttgctat tgtggcgaaa attattttca gttataggat acttttttcc
     4321 ttctgaaaag taataagctt aaagtttttg tttctgtaag atgtaatgat agtaactatt
     4381 attttgggca tattaaccaa cagaacaatt atgtagtatg tatatcactt tctcattaga
     4441 tctctaactt gaccttctag tacctttatg tgtgaggata aatttgttag atccttttat
     4501 tctcaatctt ggataatgat gcaaaatgga attttgtttt gtttgttggt agattcacat
     4561 actaaaactt tgcaggtaaa gttgcttgcc tcatgttaga atttaattag aaaactgagg
     4621 ctgccttttt tttttttttt tttttttttt aagacagagt cttgttctgt cgcccaggcc
     4681 agtttttttt ttcttgtaca gatggggttt taccatgttg ctcaggctgg tctcaaactc
     4741 ctggagtcaa gggatctatt tgcctcagcc tctcaaagtg ctgggattac aggtgtgagc
     4801 cacggctcct ggctccttgc tccagaatca gttttttaat ggaagatagc tcatactttg
     4861 tttttgtttt taaagagaca gggtctcact ctgttgccca ggctggtagt agcgtgatca
     4921 tagctcactg cagtctcaaa ctcctggact caaatagtgc tctggagtac ctagaactac
     4981 aagtgcctac caccacaccc agctaattta tttgtttttt tttttttaga gatggggggg
     5041 gtctccctat gttgcccagg ctggtctcaa actcttcacc tcaagcaaaa ctcccactgc
     5101 agaaagcttt gggattacag gcatgagcca ccatgccaag cccatactta ttgttaataa
     5161 agtttcagtg acttgcttac taatcttatg caaaatttca tgtaatttat gtaatactac
     5221 ataacaatgc tacatatggt atgtatacat acactgtata cacacaaagg tctgttctgg
     5281 gtattttatg tatcttatct ctaggcttta tggctctgaa atggagggaa ctggacataa
     5341 atgtttcaag gaataaagtg aatgttttgt ttgtttgttt gttttgagac ggagtctcag
     5401 tcaccaggct ggagtgcagt gctgtgatct cagctcactg caacctctgc ctcctgggtt
     5461 caagcgattc tcctgcctca gcctcccgag tagctggcat tacaggtgcc tgccaccatg
     5521 tccagctaat ttttgtgttt ttagtagaga tggggtttca ccatgatggt caggctggtc
     5581 tcgaactcct gacctcatga tccgccagcc tcagcctccc atagtgctgg gattataggc
     5641 atgagccatg ctcccggcca aagtgaatgt tttgggtgaa gtttatataa ggatttgact
     5701 tttgtatcat tattaaagtt tctgtcaggt aatacagtgt tggccctctg ttgaaataaa
     5761 atgttacata tcgctgggtg tagtggctca tgcctttaat cccagtactt tgggaggcca
     5821 aggcaggagg attgcttgag ctcagaagtt tgagaccagc ctgggcaaca tagcaaaacc
     5881 ttgtcactat aaaaaataca aaaattagcc aggcgtggtg gtgcacgcct gtagtctcag
     5941 ctactcagtc ggctgaggtg ggaagatggc ttcaagccgg gagatggagg cttcagtgac
     6001 ctgtgacctg tgatcacacc acttaattcc tcaaaacatt tatgtcccag ttccctccat
     6061 ttcagagcca tgggcaacag agcgagaccc tgtctcaaaa aaaaaattac atataagccc
     6121 ggtttaaaga gaggataata tgcaaccacc ctagttgaaa cagagtgtat cacttctttc
     6181 tcaccctaaa tttaatgcct agtttgaaaa tcagtagaca ataataaagt tgataagctt
     6241 taggttggta ggaaagctat gtaatctata tagtgtacta aatccttata tgtttttggt
     6301 aaaaaaagac tttaatagct aatttggact tcagaaaatg ggttaatttc acgagtgcat
     6361 atcacttttg actatattag tcaagcatat tattaaagtt taaaaaactc taaaacttct
     6421 gtactgtttt tttcttaagt gctctcttcc ttatttctac attactacat tcacatttct
     6481 gctgtcaata agccctaatg gagtgataaa ctttaagcta gtagatactg caccatgtct
     6541 tgtggtggtg ttttcttaat gaaatggaag ctgtacttcc tgtttgtttt taaatacttt
     6601 tgaaacagac agttcatgta ccccatcatt tagagatgca gtttgaattt ccatggaaaa
     6661 aggtgccaat tttggattgg gaaaggagct gaagccccag tcaagacttt gccatttggg
     6721 gtaatgcagc agattcctgt gtctgtatct ttccagtgtg tgtgttaatc catagtgttc
     6781 tgagcaccag aagggtcatg taaatctgaa gttgttatta aaggtttttc tatagtgaaa
//
