LOCUS       NM_015132               6357 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens sorting nexin 13 (SNX13), transcript variant 2, mRNA.
ACCESSION   NM_015132
VERSION     NM_015132.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Lu,A., Hsieh,F., Sharma,B.R., Vaughn,S.R., Enrich,C. and
            Pfeffer,S.R.
  TITLE     CRISPR screens for lipid regulators reveal a role for ER-bound
            SNX13 in lysosomal cholesterol export
  JOURNAL   J Cell Biol 221 (2) (2022)
   PUBMED   34936700
  REMARK    GeneRIF: CRISPR screens for lipid regulators reveal a role for
            ER-bound SNX13 in lysosomal cholesterol export.
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Mas,C., Norwood,S.J., Bugarcic,A., Kinna,G., Leneva,N., Kovtun,O.,
            Ghai,R., Ona Yanez,L.E., Davis,J.L., Teasdale,R.D. and Collins,B.M.
  TITLE     Structural basis for different phosphoinositide specificities of
            the PX domains of sorting nexins regulating G-protein signaling
  JOURNAL   J Biol Chem 289 (41), 28554-28568 (2014)
   PUBMED   25148684
REFERENCE   3  (bases 1 to 6357)
  AUTHORS   Willer,C.J., Schmidt,E.M., Sengupta,S., Peloso,G.M., Gustafsson,S.,
            Kanoni,S., Ganna,A., Chen,J., Buchkovich,M.L., Mora,S.,
            Beckmann,J.S., Bragg-Gresham,J.L., Chang,H.Y., Demirkan,A., Den
            Hertog,H.M., Do,R., Donnelly,L.A., Ehret,G.B., Esko,T.,
            Feitosa,M.F., Ferreira,T., Fischer,K., Fontanillas,P., Fraser,R.M.,
            Freitag,D.F., Gurdasani,D., Heikkila,K., Hypponen,E., Isaacs,A.,
            Jackson,A.U., Johansson,A., Johnson,T., Kaakinen,M., Kettunen,J.,
            Kleber,M.E., Li,X., Luan,J., Lyytikainen,L.P., Magnusson,P.K.E.,
            Mangino,M., Mihailov,E., Montasser,M.E., Muller-Nurasyid,M.,
            Nolte,I.M., O'Connell,J.R., Palmer,C.D., Perola,M., Petersen,A.K.,
            Sanna,S., Saxena,R., Service,S.K., Shah,S., Shungin,D., Sidore,C.,
            Song,C., Strawbridge,R.J., Surakka,I., Tanaka,T., Teslovich,T.M.,
            Thorleifsson,G., Van den Herik,E.G., Voight,B.F., Volcik,K.A.,
            Waite,L.L., Wong,A., Wu,Y., Zhang,W., Absher,D., Asiki,G.,
            Barroso,I., Been,L.F., Bolton,J.L., Bonnycastle,L.L., Brambilla,P.,
            Burnett,M.S., Cesana,G., Dimitriou,M., Doney,A.S.F., Doring,A.,
            Elliott,P., Epstein,S.E., Ingi Eyjolfsson,G., Gigante,B.,
            Goodarzi,M.O., Grallert,H., Gravito,M.L., Groves,C.J., Hallmans,G.,
            Hartikainen,A.L., Hayward,C., Hernandez,D., Hicks,A.A., Holm,H.,
            Hung,Y.J., Illig,T., Jones,M.R., Kaleebu,P., Kastelein,J.J.P.,
            Khaw,K.T., Kim,E., Klopp,N., Komulainen,P., Kumari,M.,
            Langenberg,C., Lehtimaki,T., Lin,S.Y., Lindstrom,J., Loos,R.J.F.,
            Mach,F., McArdle,W.L., Meisinger,C., Mitchell,B.D., Muller,G.,
            Nagaraja,R., Narisu,N., Nieminen,T.V.M., Nsubuga,R.N., Olafsson,I.,
            Ong,K.K., Palotie,A., Papamarkou,T., Pomilla,C., Pouta,A.,
            Rader,D.J., Reilly,M.P., Ridker,P.M., Rivadeneira,F., Rudan,I.,
            Ruokonen,A., Samani,N., Scharnagl,H., Seeley,J., Silander,K.,
            Stancakova,A., Stirrups,K., Swift,A.J., Tiret,L.,
            Uitterlinden,A.G., van Pelt,L.J., Vedantam,S., Wainwright,N.,
            Wijmenga,C., Wild,S.H., Willemsen,G., Wilsgaard,T., Wilson,J.F.,
            Young,E.H., Zhao,J.H., Adair,L.S., Arveiler,D., Assimes,T.L.,
            Bandinelli,S., Bennett,F., Bochud,M., Boehm,B.O., Boomsma,D.I.,
            Borecki,I.B., Bornstein,S.R., Bovet,P., Burnier,M., Campbell,H.,
            Chakravarti,A., Chambers,J.C., Chen,Y.I., Collins,F.S.,
            Cooper,R.S., Danesh,J., Dedoussis,G., de Faire,U., Feranil,A.B.,
            Ferrieres,J., Ferrucci,L., Freimer,N.B., Gieger,C., Groop,L.C.,
            Gudnason,V., Gyllensten,U., Hamsten,A., Harris,T.B., Hingorani,A.,
            Hirschhorn,J.N., Hofman,A., Hovingh,G.K., Hsiung,C.A.,
            Humphries,S.E., Hunt,S.C., Hveem,K., Iribarren,C., Jarvelin,M.R.,
            Jula,A., Kahonen,M., Kaprio,J., Kesaniemi,A., Kivimaki,M.,
            Kooner,J.S., Koudstaal,P.J., Krauss,R.M., Kuh,D., Kuusisto,J.,
            Kyvik,K.O., Laakso,M., Lakka,T.A., Lind,L., Lindgren,C.M.,
            Martin,N.G., Marz,W., McCarthy,M.I., McKenzie,C.A., Meneton,P.,
            Metspalu,A., Moilanen,L., Morris,A.D., Munroe,P.B., Njolstad,I.,
            Pedersen,N.L., Power,C., Pramstaller,P.P., Price,J.F., Psaty,B.M.,
            Quertermous,T., Rauramaa,R., Saleheen,D., Salomaa,V.,
            Sanghera,D.K., Saramies,J., Schwarz,P.E.H., Sheu,W.H.,
            Shuldiner,A.R., Siegbahn,A., Spector,T.D., Stefansson,K.,
            Strachan,D.P., Tayo,B.O., Tremoli,E., Tuomilehto,J., Uusitupa,M.,
            van Duijn,C.M., Vollenweider,P., Wallentin,L., Wareham,N.J.,
            Whitfield,J.B., Wolffenbuttel,B.H.R., Ordovas,J.M., Boerwinkle,E.,
            Palmer,C.N.A., Thorsteinsdottir,U., Chasman,D.I., Rotter,J.I.,
            Franks,P.W., Ripatti,S., Cupples,L.A., Sandhu,M.S., Rich,S.S.,
            Boehnke,M., Deloukas,P., Kathiresan,S., Mohlke,K.L., Ingelsson,E.
            and Abecasis,G.R.
  CONSRTM   Global Lipids Genetics Consortium
  TITLE     Discovery and refinement of loci associated with lipid levels
  JOURNAL   Nat Genet 45 (11), 1274-1283 (2013)
   PUBMED   24097068
REFERENCE   4  (bases 1 to 6357)
  AUTHORS   Hillman,R.T., Green,R.E. and Brenner,S.E.
  TITLE     An unappreciated role for RNA surveillance
  JOURNAL   Genome Biol 5 (2), R8 (2004)
   PUBMED   14759258
REFERENCE   5  (bases 1 to 6357)
  AUTHORS   Worby,C.A. and Dixon,J.E.
  TITLE     Sorting out the cellular functions of sorting nexins
  JOURNAL   Nat Rev Mol Cell Biol 3 (12), 919-931 (2002)
   PUBMED   12461558
  REMARK    Review article
            Erratum:[Nat Rev Mol Cell Biol. 2003 Feb;4(2):156.]
REFERENCE   6  (bases 1 to 6357)
  AUTHORS   Kosan,C. and Kunz,J.
  TITLE     Identification and characterisation of the gene TWIST NEIGHBOR
            (TWISTNB) located in the microdeletion syndrome 7p21 region
  JOURNAL   Cytogenet Genome Res 97 (3-4), 167-170 (2002)
   PUBMED   12438708
REFERENCE   7  (bases 1 to 6357)
  AUTHORS   Zheng,B., Ma,Y.C., Ostrom,R.S., Lavoie,C., Gill,G.N., Insel,P.A.,
            Huang,X.Y. and Farquhar,M.G.
  TITLE     RGS-PX1, a GAP for GalphaS and sorting nexin in vesicular
            trafficking
  JOURNAL   Science 294 (5548), 1939-1942 (2001)
   PUBMED   11729322
REFERENCE   8  (bases 1 to 6357)
  AUTHORS   Teasdale,R.D., Loci,D., Houghton,F., Karlsson,L. and Gleeson,P.A.
  TITLE     A large family of endosome-localized proteins related to sorting
            nexin 1
  JOURNAL   Biochem J 358 (Pt 1), 7-16 (2001)
   PUBMED   11485546
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB050735.1, AF420470.1,
            BC045667.1 and AC073205.6.
            
            On Nov 23, 2018 this sequence version replaced NM_015132.4.
            
            Summary: This gene encodes a PHOX domain- and RGS domain-containing
            protein that belongs to the sorting nexin (SNX) family and the
            regulator of G protein signaling (RGS) family. The PHOX domain is a
            phosphoinositide binding domain, and the SNX family members are
            involved in intracellular trafficking. The RGS family members are
            regulatory molecules that act as GTPase activating proteins for G
            alpha subunits of heterotrimeric G proteins. The RGS domain of this
            protein interacts with G alpha(s), accelerates its GTP hydrolysis,
            and attenuates G alpha(s)-mediated signaling. Overexpression of
            this protein delayes lysosomal degradation of the epidermal growth
            factor receptor. Because of its bifunctional role, this protein may
            link heterotrimeric G protein signaling and vesicular trafficking.
            [provided by RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.264128.1,
                                           SRR1803614.113921.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000428135.7/ ENSP00000398789.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                DB050735.1         25-42
            19-3402             AF420470.1         1-3384
            3403-4132           BC045667.1         3387-4116
            4133-6357           AC073205.6         15584-17808         c
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p21.1"
     gene            1..6357
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /note="sorting nexin 13"
                     /db_xref="GeneID:23161"
                     /db_xref="HGNC:HGNC:21335"
                     /db_xref="MIM:606589"
     exon            1..211
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..3073
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /note="isoform b is encoded by transcript variant 2; rgs
                     domain- and phox domain-containing protein"
                     /codon_start=1
                     /product="sorting nexin-13 isoform b"
                     /protein_id="NP_055947.1"
                     /db_xref="CCDS:CCDS47551.1"
                     /db_xref="GeneID:23161"
                     /db_xref="HGNC:HGNC:21335"
                     /db_xref="MIM:606589"
                     /translation="MLTEASLSIWGWGSLGIVLFLITFGPFVIFYLTFYILCFVGGGL
                     VVTLLFGKTNSEKYLEQCEHSFLPPTSPGVPKCLEEMKREARTIKIDRRLTGANIIDE
                     PLQQVIQFSLRDYVQYWYYTLSDDESFLLEIRQTLQNALIQFATRSKEIDWQPYFTTR
                     IVDDFGTHLRVFRKAQQKITEKDDQVKGTAEDLVDTFFEVEVEMEKEVCRDLVCTSPK
                     DEEGFLRDLCEVLLYLLLPPGDFQNKIMRYFVREILARGILLPLINQLSDPDYINQYV
                     IWMIRDSNCNYEAFMNIIKLSDNIGELEAVRDKAAEELQYLRSLDTAGDDINTIKNQI
                     NSLLFVKKVCDSRIQRLQSGKEINTVKLAANFGKLCTVPLDSILVDNVALQFFMDYMQ
                     QTGGQAHLFFWMTVEGYRVTAQQQLEVLLSRQRDGKHQTNQTKGLLRAAAVGIYEQYL
                     SEKASPRVTVDDYLVAKLADTLNHEDPTPEIFDDIQRKVYELMLRDERFYPSFRQNAL
                     YVRMLAELDMLKDPSFRGSDDGDGESFNGSPTGSINLSLDDLSNVSSDDSVQLHAYIS
                     DTGVCNDHGKTYALYAITVHRRNLNSEEMWKTYRRYSDFHDFHMRITEQFESLSSILK
                     LPGKKTFNNMDRDFLEKRKKDLNAYLQLLLAPEMMKASPALAHYVYDFLENKAYSKGK
                     GDFARKMDTFVNPLRNSMRNVSNAVKSLPDSLAEGMTKMSDNMGKMSERLGQDIKQSF
                     FKVPPLIPKTDSDPEHRRVSAQLDDNVDDNIPLRVMLLLMDEVFDLKERNQWLRRNIK
                     NLLQQLIRATYGDTINRKIVDHVDWMTSPEQVADSVKRFRDAFWPNGILAEAVPCRDK
                     SIRMRTRVAGKTKLLAIMPDELKHIIGAETTRKGILRVFEMFQHNQLNRRMVYVFLEG
                     FLETLFPQYKFRELFNKLHSRSKQMQKYKQKLQTTQAPSLQKR"
     exon            212..324
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            325..427
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            428..517
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            518..639
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            640..761
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            762..863
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            864..952
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1036
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1175
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1264
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1364
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1558
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1559..1663
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1664..1796
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..1834
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1835..1904
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..2044
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2045..2152
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2263
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2264..2425
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2426..2497
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2498..2643
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2644..2712
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2713..2825
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2826..6357
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4260..4265
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /note="hexamer: AATAAA"
     polyA_site      4277
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /note="major polyA site"
     regulatory      6339..6344
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /note="hexamer: AATAAA"
     polyA_site      6357
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /note="major polyA site"
ORIGIN      
        1 gctcttccaa gatggcggcg cgtccgtcgc gagcgaccgg gccgagggga ggccagcgaa
       61 gccgagtaaa accgccgccc gggagaagac tgaaggagca gttgccgccg ttggcggcgg
      121 cccgagcagt tttcgctgct gctacggctg ttgccatgag gcgaggctag ggaggacctc
      181 acttccccgg ggtgtaataa tgttaactga ggccagtcta tccatatggg gatggggaag
      241 ccttggcatt gtcctttttc tgataacctt tggacccttt gtaatatttt atttgacatt
      301 ttatatcctc tgctttgtgg gtgggggttt agtggttact ctcctgtttg gaaaaacaaa
      361 ctcagagaag tacctagaac agtgtgaaca ctcatttctt cctccaacat cacctggggt
      421 tcctaagtgc ttagaagaaa tgaaacggga agccaggact attaagattg atagaagatt
      481 gacgggtgcc aatataattg atgaacctct ccagcaagtt atccagtttt ccttgaggga
      541 ttatgtccag tattggtatt atacactaag cgatgatgaa tcttttcttc ttgaaattag
      601 gcagactctt caaaacgcac tcattcagtt tgctactagg tcaaaagaaa tagactggca
      661 accttatttt actacacgca ttgtagatga ctttggcaca cacttacgag tattcagaaa
      721 ggctcaacag aaaataacag agaaagatga tcaagtgaaa ggtacagcag aagatcttgt
      781 agataccttc tttgaagttg aagttgaaat ggagaaggag gtttgccgtg atctagtgtg
      841 cacttccccc aaagatgaag aaggattcct aagggatttg tgtgaggtct tactatattt
      901 attgctacct cctggagatt tccagaacaa gatcatgcga tactttgtca gggaaatcct
      961 tgcacgagga attcttcttc cattaataaa tcaactcagt gatcctgatt atattaatca
     1021 gtatgtcata tggatgatcc gtgattctaa ctgcaactat gaggccttta tgaacattat
     1081 taaattgagt gacaatattg gagagctaga agcagtcaga gataaggcag cagaagaatt
     1141 acagtatctt agatctctag atacagctgg tgatgatatc aacactatca aaaatcaaat
     1201 aaatagctta ctattcgtaa agaaggtatg tgactcaaga atacagcgat tgcagtcagg
     1261 caaagaaata aatactgtga aacttgcagc aaactttggg aaactttgca cagtccccct
     1321 ggacagcatt cttgtagaca atgttgcact acaatttttt atggattaca tgcagcaaac
     1381 tggaggtcaa gcacatctat tcttttggat gacagtggaa ggataccggg ttaccgccca
     1441 acagcagcta gaagttttat taagtcgtca gagagatgga aaacatcaaa ccaaccaaac
     1501 caaaggtctt ttaagagcag ctgctgttgg aatttatgaa cagtatttat cagaaaaggc
     1561 atctccaaga gttactgttg atgactattt agtagcaaaa ttagcagata ctttgaatca
     1621 tgaagatcca acccctgaaa tctttgatga cattcaaaga aaggtatatg aattgatgct
     1681 acgagatgaa agattttatc cttccttcag acagaatgca ctttatgtgc gcatgttagc
     1741 tgagcttgac atgttaaaag atcctagttt cagaggatcc gatgatgggg atggagaatc
     1801 ttttaatggg tctcctacag gaagcataaa tttgtcttta gatgaccttt caaatgtttc
     1861 ttctgatgac tcagtacaac ttcatgccta catttcagac acaggcgttt gtaatgatca
     1921 tggcaagaca tatgcattat atgccatcac tgtacaccgg cgcaacctaa acagtgagga
     1981 gatgtggaaa acctatcgtc gttatagtga cttccatgac ttccacatga gaatcactga
     2041 acagtttgaa agtctctcaa gcatattgaa acttcctgga aaaaagactt ttaataatat
     2101 ggatagagat tttttagaaa agagaaaaaa ggatctaaat gcatatttgc agttactgtt
     2161 agctcctgaa atgatgaagg catcccccgc tttagctcac tatgtgtatg atttccttga
     2221 gaacaaagcc tacagtaaag gaaaagggga ttttgctcgc aagatggaca cttttgtaaa
     2281 tccacttcgc aattcaatga ggaatgtttc aaatgcagtt aaatcccttc ctgatagctt
     2341 ggcagaggga atgactaaaa tgtcagacaa catgggcaaa atgtcagaaa gattaggtca
     2401 agacataaag caatcatttt ttaaggtgcc tcctttaatt cctaagactg attcagaccc
     2461 tgaacatcgc cgagtttcgg ctcaacttga cgataatgtg gatgacaata ttccacttag
     2521 ggtaatgctg cttctcatgg atgaagtatt cgacttaaaa gaaagaaatc agtggttgcg
     2581 aaggaatatc aaaaacctac ttcaacagct tattagagct acatatggcg atactattaa
     2641 tagaaaaata gttgaccatg ttgactggat gacttcacct gaacaagtag ccgactcagt
     2701 gaaacgtttc agagatgcat tttggccaaa tggcatttta gcagaggctg ttccatgcag
     2761 agataaaagt attcgaatga gaacaagagt agcaggaaaa acgaaattac ttgcaattat
     2821 gccagatgag ctgaagcaca ttattggggc tgagacaaca cggaaaggta ttcttcgtgt
     2881 ttttgaaatg tttcagcaca accaattaaa taggagaatg gtttatgtct tcttggaagg
     2941 ctttttagaa accttatttc cacagtataa attccgtgaa cttttcaaca aactgcattc
     3001 acggtcaaag cagatgcaga aatataaaca gaaacttcaa actactcaag cgccttcttt
     3061 gcagaaaagg tgacactcca ctctatgaag ctggtgttca ttttgtccag gactaatggt
     3121 atggacagat cttctggggc ttaactcaaa tactgttgtg tctgcaccag tcttttagtg
     3181 tctcaaaata gattattaat acatccataa gtctgtggtt cttctcattc tcatctataa
     3241 tccaccacta ccaagagaga tttctgtgtc ttaaatgatt tggtgcatat tttgcaacat
     3301 tgagagaaaa ctgcccccat ctcaagcaag aatggcgttg gtttcttcca tttcaaacta
     3361 atcagcattc tccagcaatg acaaagtgcc agagtgtata tatgaaagct aaggaaagca
     3421 caaaacaatg gttcagagat aaattggcta atttgtttaa cagttggaga ctgtgcaatg
     3481 tacgacttgg aggagtttgt ttggcataat gattttgtag gtctgtcagc attttgatat
     3541 attgtggatt agccaggtga atgatccttg aaacatctct ttcaggtctg gagaaagaga
     3601 cagaatgccc ttaaagtgtg aaataacctt atacctgaat tacagttttg tgatataaaa
     3661 taaagctgag tataaaataa aaccaatcaa acacagaatt tgattggatt tgttattcat
     3721 tggtatacaa caatttttat tcttatgtgt gctttaaggg ctgtgttttg ctcttagggt
     3781 actcaatttt atgtttcctt ttggcattct ttgtgcaaaa agaagcatgt ggtggctctc
     3841 atttaaattt caaaattatt agttttatct ttaacagatt actgattata cagaagatat
     3901 aatttattgg aagaactgcc aaatacattt tgtccttcta aaaatatgga aatttaaata
     3961 gtagtctgga aagttaccat atgataattt ttaattgtgt gatctagaca tgccttaggt
     4021 aactgcagaa atagcaaatt aaatgattaa tgagcttatt agtcatagta aacatacaga
     4081 ttcctaattc tatgacagtg tacatatatg aaaataaatg atgaaaaaag aactataata
     4141 tttttaaaaa tatgctttta tttatatttc acataatgta aagttctaca aaataaagct
     4201 agtaggcaag acccctcagt gtataccttt aaacccataa attaatttta tatcaataaa
     4261 ataaaatact ttctctagtt ttggagcttc tgtatttcct atagttttct gactgtccat
     4321 tttatccatc ttagaagtat tcacatccct tctatgcagg atttctgcat agttttaaat
     4381 actttcattg cctaagggtt tgggtgtgtc agcttttttt gttttaaaaa tatttaacac
     4441 taaaacctta aaataatgaa gctacttatc agaccactga gccaagatcc tggtattaaa
     4501 agaaagtctg ggtgcttaag ataaagggac agagtattgg tatcccagtt atttgggagc
     4561 tttaagattg gaacaagata tcactgtctt gttttcactt agatcctact tacaaagtga
     4621 gggttattaa cagaataaag ccttccttta aagctttata ataatcatat ttattaataa
     4681 tgctgttgtg catacttata gtatgcatat attcagcata tgttgcatgt cttcagaatt
     4741 acataaaatg aaatcccttt cattgcaact tgcaagtgag aaaagatcct tagtggctct
     4801 ggtggaagaa atagtatttc ttcttctcag ggtgtctccc tgccttggcc cctccctgag
     4861 ccccaggctt taaaagtgaa aatgtttgaa acatgaaaca tgtctgtagg aagcatcagc
     4921 atggccataa gtgcagtgat tttcatatat gcctctgccc atttcaaata tatttttgac
     4981 atgaataaat ctaacagtat acagaataat tcatgtaaga ccctaacgtg tacatgtgaa
     5041 aaagcatttc tatataatgt gaggagcact ggccatcaat tagggaaata aaggtcatgt
     5101 aatattgcaa attttcaaaa tagagccctg caagataact gcaatcatac caaaaactat
     5161 ttgagtaaat ggatttttaa agtaattttt gtttagaaga gtttatattt cagaagcaga
     5221 aaatgtcaaa tgatagtctt tgtaaatggt ggtgcacctt ctctatgcac atctgtcttt
     5281 tacatcagaa agagctgtgg tcatgctaaa attagagata actttttgaa tgacttggtc
     5341 aagctgtgtg taaaatattt aaccataagt caagtacagt gtactatgtt taataaagtt
     5401 acatttaatg catttattgc atatatgaat atatacatga agaggcttta tgtcttctgg
     5461 tatttgattt tgaatgtttt ttaagtcagt ggtgccttta ggcaagaact ttcgaaatta
     5521 atcattcttt gtgttttctg atttttcagg taacatgtac actatttaga aaccatcata
     5581 gtttattcac cttaaaaaat tgattgtatt atttaaatat atcacttaga tgggcatttc
     5641 ctataattag gatattccaa atagttgctg aaatcaattg tgccattgac caatggatgc
     5701 acttggttag ccttaatttt tttaaaaaaa aaaaaaacaa aaactttctt gaatatttca
     5761 gtttgttcat tttaagtttg tgctgctggt atttgggaag aaaaatcaat cagttaagtg
     5821 ctaccagaaa gtgtaccaat ttttataaaa attaagaatg atataaattt cacaatctaa
     5881 aattttaatt ttgtgcaata ttttcattta atgcatgtgt atttgagtaa ttgtaagata
     5941 aatgaatttt taattttttg agatgtagct taaactgtct tcttaatcca acaacttaca
     6001 ttccgttgtt gctgcatcct tttatttaag gacttgtttt aaaagtcttt ttgtcactcc
     6061 ttggaaaatt cttttattag tagtaaattt tgcttttagg agcatggctc ctgtattaca
     6121 agggaaaaaa tatgaagagc acattttagg attataattg ttaaaagata atgtgtgcat
     6181 atcattgtac agtaagtgtc aactgtgtgc ctaactgttc tatcaatact ttctttcttt
     6241 aacaaagaag aaacaacaat cagaatgtca gttattttta ttcaactaaa aggattaaaa
     6301 aatttatttg gtttgtgttc tgtccattag aaaatactaa taaagtatta acaaaca
//
