LOCUS       NM_001350862            6390 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens sorting nexin 13 (SNX13), transcript variant 1, mRNA.
ACCESSION   NM_001350862 XM_005249672
VERSION     NM_001350862.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Lu,A., Hsieh,F., Sharma,B.R., Vaughn,S.R., Enrich,C. and
            Pfeffer,S.R.
  TITLE     CRISPR screens for lipid regulators reveal a role for ER-bound
            SNX13 in lysosomal cholesterol export
  JOURNAL   J Cell Biol 221 (2) (2022)
   PUBMED   34936700
  REMARK    GeneRIF: CRISPR screens for lipid regulators reveal a role for
            ER-bound SNX13 in lysosomal cholesterol export.
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   Mas,C., Norwood,S.J., Bugarcic,A., Kinna,G., Leneva,N., Kovtun,O.,
            Ghai,R., Ona Yanez,L.E., Davis,J.L., Teasdale,R.D. and Collins,B.M.
  TITLE     Structural basis for different phosphoinositide specificities of
            the PX domains of sorting nexins regulating G-protein signaling
  JOURNAL   J Biol Chem 289 (41), 28554-28568 (2014)
   PUBMED   25148684
REFERENCE   3  (bases 1 to 6390)
  AUTHORS   Willer,C.J., Schmidt,E.M., Sengupta,S., Peloso,G.M., Gustafsson,S.,
            Kanoni,S., Ganna,A., Chen,J., Buchkovich,M.L., Mora,S.,
            Beckmann,J.S., Bragg-Gresham,J.L., Chang,H.Y., Demirkan,A., Den
            Hertog,H.M., Do,R., Donnelly,L.A., Ehret,G.B., Esko,T.,
            Feitosa,M.F., Ferreira,T., Fischer,K., Fontanillas,P., Fraser,R.M.,
            Freitag,D.F., Gurdasani,D., Heikkila,K., Hypponen,E., Isaacs,A.,
            Jackson,A.U., Johansson,A., Johnson,T., Kaakinen,M., Kettunen,J.,
            Kleber,M.E., Li,X., Luan,J., Lyytikainen,L.P., Magnusson,P.K.E.,
            Mangino,M., Mihailov,E., Montasser,M.E., Muller-Nurasyid,M.,
            Nolte,I.M., O'Connell,J.R., Palmer,C.D., Perola,M., Petersen,A.K.,
            Sanna,S., Saxena,R., Service,S.K., Shah,S., Shungin,D., Sidore,C.,
            Song,C., Strawbridge,R.J., Surakka,I., Tanaka,T., Teslovich,T.M.,
            Thorleifsson,G., Van den Herik,E.G., Voight,B.F., Volcik,K.A.,
            Waite,L.L., Wong,A., Wu,Y., Zhang,W., Absher,D., Asiki,G.,
            Barroso,I., Been,L.F., Bolton,J.L., Bonnycastle,L.L., Brambilla,P.,
            Burnett,M.S., Cesana,G., Dimitriou,M., Doney,A.S.F., Doring,A.,
            Elliott,P., Epstein,S.E., Ingi Eyjolfsson,G., Gigante,B.,
            Goodarzi,M.O., Grallert,H., Gravito,M.L., Groves,C.J., Hallmans,G.,
            Hartikainen,A.L., Hayward,C., Hernandez,D., Hicks,A.A., Holm,H.,
            Hung,Y.J., Illig,T., Jones,M.R., Kaleebu,P., Kastelein,J.J.P.,
            Khaw,K.T., Kim,E., Klopp,N., Komulainen,P., Kumari,M.,
            Langenberg,C., Lehtimaki,T., Lin,S.Y., Lindstrom,J., Loos,R.J.F.,
            Mach,F., McArdle,W.L., Meisinger,C., Mitchell,B.D., Muller,G.,
            Nagaraja,R., Narisu,N., Nieminen,T.V.M., Nsubuga,R.N., Olafsson,I.,
            Ong,K.K., Palotie,A., Papamarkou,T., Pomilla,C., Pouta,A.,
            Rader,D.J., Reilly,M.P., Ridker,P.M., Rivadeneira,F., Rudan,I.,
            Ruokonen,A., Samani,N., Scharnagl,H., Seeley,J., Silander,K.,
            Stancakova,A., Stirrups,K., Swift,A.J., Tiret,L.,
            Uitterlinden,A.G., van Pelt,L.J., Vedantam,S., Wainwright,N.,
            Wijmenga,C., Wild,S.H., Willemsen,G., Wilsgaard,T., Wilson,J.F.,
            Young,E.H., Zhao,J.H., Adair,L.S., Arveiler,D., Assimes,T.L.,
            Bandinelli,S., Bennett,F., Bochud,M., Boehm,B.O., Boomsma,D.I.,
            Borecki,I.B., Bornstein,S.R., Bovet,P., Burnier,M., Campbell,H.,
            Chakravarti,A., Chambers,J.C., Chen,Y.I., Collins,F.S.,
            Cooper,R.S., Danesh,J., Dedoussis,G., de Faire,U., Feranil,A.B.,
            Ferrieres,J., Ferrucci,L., Freimer,N.B., Gieger,C., Groop,L.C.,
            Gudnason,V., Gyllensten,U., Hamsten,A., Harris,T.B., Hingorani,A.,
            Hirschhorn,J.N., Hofman,A., Hovingh,G.K., Hsiung,C.A.,
            Humphries,S.E., Hunt,S.C., Hveem,K., Iribarren,C., Jarvelin,M.R.,
            Jula,A., Kahonen,M., Kaprio,J., Kesaniemi,A., Kivimaki,M.,
            Kooner,J.S., Koudstaal,P.J., Krauss,R.M., Kuh,D., Kuusisto,J.,
            Kyvik,K.O., Laakso,M., Lakka,T.A., Lind,L., Lindgren,C.M.,
            Martin,N.G., Marz,W., McCarthy,M.I., McKenzie,C.A., Meneton,P.,
            Metspalu,A., Moilanen,L., Morris,A.D., Munroe,P.B., Njolstad,I.,
            Pedersen,N.L., Power,C., Pramstaller,P.P., Price,J.F., Psaty,B.M.,
            Quertermous,T., Rauramaa,R., Saleheen,D., Salomaa,V.,
            Sanghera,D.K., Saramies,J., Schwarz,P.E.H., Sheu,W.H.,
            Shuldiner,A.R., Siegbahn,A., Spector,T.D., Stefansson,K.,
            Strachan,D.P., Tayo,B.O., Tremoli,E., Tuomilehto,J., Uusitupa,M.,
            van Duijn,C.M., Vollenweider,P., Wallentin,L., Wareham,N.J.,
            Whitfield,J.B., Wolffenbuttel,B.H.R., Ordovas,J.M., Boerwinkle,E.,
            Palmer,C.N.A., Thorsteinsdottir,U., Chasman,D.I., Rotter,J.I.,
            Franks,P.W., Ripatti,S., Cupples,L.A., Sandhu,M.S., Rich,S.S.,
            Boehnke,M., Deloukas,P., Kathiresan,S., Mohlke,K.L., Ingelsson,E.
            and Abecasis,G.R.
  CONSRTM   Global Lipids Genetics Consortium
  TITLE     Discovery and refinement of loci associated with lipid levels
  JOURNAL   Nat Genet 45 (11), 1274-1283 (2013)
   PUBMED   24097068
REFERENCE   4  (bases 1 to 6390)
  AUTHORS   Hillman,R.T., Green,R.E. and Brenner,S.E.
  TITLE     An unappreciated role for RNA surveillance
  JOURNAL   Genome Biol 5 (2), R8 (2004)
   PUBMED   14759258
REFERENCE   5  (bases 1 to 6390)
  AUTHORS   Worby,C.A. and Dixon,J.E.
  TITLE     Sorting out the cellular functions of sorting nexins
  JOURNAL   Nat Rev Mol Cell Biol 3 (12), 919-931 (2002)
   PUBMED   12461558
  REMARK    Review article
            Erratum:[Nat Rev Mol Cell Biol. 2003 Feb;4(2):156.]
REFERENCE   6  (bases 1 to 6390)
  AUTHORS   Kosan,C. and Kunz,J.
  TITLE     Identification and characterisation of the gene TWIST NEIGHBOR
            (TWISTNB) located in the microdeletion syndrome 7p21 region
  JOURNAL   Cytogenet Genome Res 97 (3-4), 167-170 (2002)
   PUBMED   12438708
REFERENCE   7  (bases 1 to 6390)
  AUTHORS   Zheng,B., Ma,Y.C., Ostrom,R.S., Lavoie,C., Gill,G.N., Insel,P.A.,
            Huang,X.Y. and Farquhar,M.G.
  TITLE     RGS-PX1, a GAP for GalphaS and sorting nexin in vesicular
            trafficking
  JOURNAL   Science 294 (5548), 1939-1942 (2001)
   PUBMED   11729322
REFERENCE   8  (bases 1 to 6390)
  AUTHORS   Teasdale,R.D., Loci,D., Houghton,F., Karlsson,L. and Gleeson,P.A.
  TITLE     A large family of endosome-localized proteins related to sorting
            nexin 1
  JOURNAL   Biochem J 358 (Pt 1), 7-16 (2001)
   PUBMED   11485546
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC080080.5 and AC073205.6.
            
            On May 31, 2019 this sequence version replaced NM_001350862.1.
            
            Summary: This gene encodes a PHOX domain- and RGS domain-containing
            protein that belongs to the sorting nexin (SNX) family and the
            regulator of G protein signaling (RGS) family. The PHOX domain is a
            phosphoinositide binding domain, and the SNX family members are
            involved in intracellular trafficking. The RGS family members are
            regulatory molecules that act as GTPase activating proteins for G
            alpha subunits of heterotrimeric G proteins. The RGS domain of this
            protein interacts with G alpha(s), accelerates its GTP hydrolysis,
            and attenuates G alpha(s)-mediated signaling. Overexpression of
            this protein delayes lysosomal degradation of the epidermal growth
            factor receptor. Because of its bifunctional role, this protein may
            link heterotrimeric G protein signaling and vesicular trafficking.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.14113.1,
                                           SRR1803614.214467.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-211               AC080080.5         17192-17402         c
            212-324             AC073205.6         122156-122268       c
            325-427             AC073205.6         118154-118256       c
            428-517             AC073205.6         116368-116457       c
            518-639             AC073205.6         115185-115306       c
            640-761             AC073205.6         100491-100612       c
            762-863             AC073205.6         100302-100403       c
            864-952             AC073205.6         98350-98438         c
            953-1036            AC073205.6         93229-93312         c
            1037-1175           AC073205.6         75648-75786         c
            1176-1264           AC073205.6         75169-75257         c
            1265-1364           AC073205.6         70417-70516         c
            1365-1558           AC073205.6         64629-64822         c
            1559-1663           AC073205.6         59583-59687         c
            1664-1796           AC073205.6         58874-59006         c
            1797-1834           AC073205.6         54832-54869         c
            1835-1904           AC073205.6         50844-50913         c
            1905-2077           AC073205.6         46331-46503         c
            2078-2185           AC073205.6         41004-41111         c
            2186-2296           AC073205.6         39656-39766         c
            2297-2458           AC073205.6         28241-28402         c
            2459-2530           AC073205.6         26410-26481         c
            2531-2676           AC073205.6         23831-23976         c
            2677-2745           AC073205.6         23512-23580         c
            2746-2858           AC073205.6         21649-21761         c
            2859-6390           AC073205.6         15584-19115         c
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p21.1"
     gene            1..6390
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /note="sorting nexin 13"
                     /db_xref="GeneID:23161"
                     /db_xref="HGNC:HGNC:21335"
                     /db_xref="MIM:606589"
     exon            1..211
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..3106
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /note="isoform a is encoded by transcript variant 1; rgs
                     domain- and phox domain-containing protein"
                     /codon_start=1
                     /product="sorting nexin-13 isoform a"
                     /protein_id="NP_001337791.1"
                     /db_xref="GeneID:23161"
                     /db_xref="HGNC:HGNC:21335"
                     /db_xref="MIM:606589"
                     /translation="MLTEASLSIWGWGSLGIVLFLITFGPFVIFYLTFYILCFVGGGL
                     VVTLLFGKTNSEKYLEQCEHSFLPPTSPGVPKCLEEMKREARTIKIDRRLTGANIIDE
                     PLQQVIQFSLRDYVQYWYYTLSDDESFLLEIRQTLQNALIQFATRSKEIDWQPYFTTR
                     IVDDFGTHLRVFRKAQQKITEKDDQVKGTAEDLVDTFFEVEVEMEKEVCRDLVCTSPK
                     DEEGFLRDLCEVLLYLLLPPGDFQNKIMRYFVREILARGILLPLINQLSDPDYINQYV
                     IWMIRDSNCNYEAFMNIIKLSDNIGELEAVRDKAAEELQYLRSLDTAGDDINTIKNQI
                     NSLLFVKKVCDSRIQRLQSGKEINTVKLAANFGKLCTVPLDSILVDNVALQFFMDYMQ
                     QTGGQAHLFFWMTVEGYRVTAQQQLEVLLSRQRDGKHQTNQTKGLLRAAAVGIYEQYL
                     SEKASPRVTVDDYLVAKLADTLNHEDPTPEIFDDIQRKVYELMLRDERFYPSFRQNAL
                     YVRMLAELDMLKDPSFRGSDDGDGESFNGSPTGSINLSLDDLSNVSSDDSVQLHAYIS
                     DTVYADYDPYAVAGVCNDHGKTYALYAITVHRRNLNSEEMWKTYRRYSDFHDFHMRIT
                     EQFESLSSILKLPGKKTFNNMDRDFLEKRKKDLNAYLQLLLAPEMMKASPALAHYVYD
                     FLENKAYSKGKGDFARKMDTFVNPLRNSMRNVSNAVKSLPDSLAEGMTKMSDNMGKMS
                     ERLGQDIKQSFFKVPPLIPKTDSDPEHRRVSAQLDDNVDDNIPLRVMLLLMDEVFDLK
                     ERNQWLRRNIKNLLQQLIRATYGDTINRKIVDHVDWMTSPEQVADSVKRFRDAFWPNG
                     ILAEAVPCRDKSIRMRTRVAGKTKLLAIMPDELKHIIGAETTRKGILRVFEMFQHNQL
                     NRRMVYVFLEGFLETLFPQYKFRELFNKLHSRSKQMQKYKQKLQTTQAPSLQKR"
     exon            212..324
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            325..427
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            428..517
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            518..639
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            640..761
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            762..863
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            864..952
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1036
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1175
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1264
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1364
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1558
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1559..1663
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1664..1796
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..1834
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1835..1904
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..2077
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2078..2185
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2186..2296
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2297..2458
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2459..2530
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2531..2676
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2677..2745
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2746..2858
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2859..6390
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4293..4298
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /note="hexamer: AATAAA"
     polyA_site      4310
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /note="major polyA site"
     regulatory      6372..6377
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /note="hexamer: AATAAA"
     polyA_site      6390
                     /gene="SNX13"
                     /gene_synonym="RGS-PX1"
                     /note="major polyA site"
ORIGIN      
        1 gctcttccaa gatggcggcg cgtccgtcgc gagcgaccgg gccgagggga ggccagcgaa
       61 gccgagtaaa accgccgccc gggagaagac tgaaggagca gttgccgccg ttggcggcgg
      121 cccgagcagt tttcgctgct gctacggctg ttgccatgag gcgaggctag ggaggacctc
      181 acttccccgg ggtgtaataa tgttaactga ggccagtcta tccatatggg gatggggaag
      241 ccttggcatt gtcctttttc tgataacctt tggacccttt gtaatatttt atttgacatt
      301 ttatatcctc tgctttgtgg gtgggggttt agtggttact ctcctgtttg gaaaaacaaa
      361 ctcagagaag tacctagaac agtgtgaaca ctcatttctt cctccaacat cacctggggt
      421 tcctaagtgc ttagaagaaa tgaaacggga agccaggact attaagattg atagaagatt
      481 gacgggtgcc aatataattg atgaacctct ccagcaagtt atccagtttt ccttgaggga
      541 ttatgtccag tattggtatt atacactaag cgatgatgaa tcttttcttc ttgaaattag
      601 gcagactctt caaaacgcac tcattcagtt tgctactagg tcaaaagaaa tagactggca
      661 accttatttt actacacgca ttgtagatga ctttggcaca cacttacgag tattcagaaa
      721 ggctcaacag aaaataacag agaaagatga tcaagtgaaa ggtacagcag aagatcttgt
      781 agataccttc tttgaagttg aagttgaaat ggagaaggag gtttgccgtg atctagtgtg
      841 cacttccccc aaagatgaag aaggattcct aagggatttg tgtgaggtct tactatattt
      901 attgctacct cctggagatt tccagaacaa gatcatgcga tactttgtca gggaaatcct
      961 tgcacgagga attcttcttc cattaataaa tcaactcagt gatcctgatt atattaatca
     1021 gtatgtcata tggatgatcc gtgattctaa ctgcaactat gaggccttta tgaacattat
     1081 taaattgagt gacaatattg gagagctaga agcagtcaga gataaggcag cagaagaatt
     1141 acagtatctt agatctctag atacagctgg tgatgatatc aacactatca aaaatcaaat
     1201 aaatagctta ctattcgtaa agaaggtatg tgactcaaga atacagcgat tgcagtcagg
     1261 caaagaaata aatactgtga aacttgcagc aaactttggg aaactttgca cagtccccct
     1321 ggacagcatt cttgtagaca atgttgcact acaatttttt atggattaca tgcagcaaac
     1381 tggaggtcaa gcacatctat tcttttggat gacagtggaa ggataccggg ttaccgccca
     1441 acagcagcta gaagttttat taagtcgtca gagagatgga aaacatcaaa ccaaccaaac
     1501 caaaggtctt ttaagagcag ctgctgttgg aatttatgaa cagtatttat cagaaaaggc
     1561 atctccaaga gttactgttg atgactattt agtagcaaaa ttagcagata ctttgaatca
     1621 tgaagatcca acccctgaaa tctttgatga cattcaaaga aaggtatatg aattgatgct
     1681 acgagatgaa agattttatc cttccttcag acagaatgca ctttatgtgc gcatgttagc
     1741 tgagcttgac atgttaaaag atcctagttt cagaggatcc gatgatgggg atggagaatc
     1801 ttttaatggg tctcctacag gaagcataaa tttgtcttta gatgaccttt caaatgtttc
     1861 ttctgatgac tcagtacaac ttcatgccta catttcagac acagtatatg ctgactatga
     1921 cccatatgct gtggcaggcg tttgtaatga tcatggcaag acatatgcat tatatgccat
     1981 cactgtacac cggcgcaacc taaacagtga ggagatgtgg aaaacctatc gtcgttatag
     2041 tgacttccat gacttccaca tgagaatcac tgaacagttt gaaagtctct caagcatatt
     2101 gaaacttcct ggaaaaaaga cttttaataa tatggataga gattttttag aaaagagaaa
     2161 aaaggatcta aatgcatatt tgcagttact gttagctcct gaaatgatga aggcatcccc
     2221 cgctttagct cactatgtgt atgatttcct tgagaacaaa gcctacagta aaggaaaagg
     2281 ggattttgct cgcaagatgg acacttttgt aaatccactt cgcaattcaa tgaggaatgt
     2341 ttcaaatgca gttaaatccc ttcctgatag cttggcagag ggaatgacta aaatgtcaga
     2401 caacatgggc aaaatgtcag aaagattagg tcaagacata aagcaatcat tttttaaggt
     2461 gcctccttta attcctaaga ctgattcaga ccctgaacat cgccgagttt cggctcaact
     2521 tgacgataat gtggatgaca atattccact tagggtaatg ctgcttctca tggatgaagt
     2581 attcgactta aaagaaagaa atcagtggtt gcgaaggaat atcaaaaacc tacttcaaca
     2641 gcttattaga gctacatatg gcgatactat taatagaaaa atagttgacc atgttgactg
     2701 gatgacttca cctgaacaag tagccgactc agtgaaacgt ttcagagatg cattttggcc
     2761 aaatggcatt ttagcagagg ctgttccatg cagagataaa agtattcgaa tgagaacaag
     2821 agtagcagga aaaacgaaat tacttgcaat tatgccagat gagctgaagc acattattgg
     2881 ggctgagaca acacggaaag gtattcttcg tgtttttgaa atgtttcagc acaaccaatt
     2941 aaataggaga atggtttatg tcttcttgga aggcttttta gaaaccttat ttccacagta
     3001 taaattccgt gaacttttca acaaactgca ttcacggtca aagcagatgc agaaatataa
     3061 acagaaactt caaactactc aagcgccttc tttgcagaaa aggtgacact ccactctatg
     3121 aagctggtgt tcattttgtc caggactaat ggtatggaca gatcttctgg ggcttaactc
     3181 aaatactgtt gtgtctgcac cagtctttta gtgtctcaaa atagattatt aatacatcca
     3241 taagtctgtg gttcttctca ttctcatcta taatccacca ctaccaagag agatttctgt
     3301 gtcttaaatg atttggtgca tattttgcaa cattgagaga aaactgcccc catctcaagc
     3361 aagaatggcg ttggtttctt ccatttcaaa ctaatcagca ttctccagca atgacaaagt
     3421 gccagagtgt atatatgaaa gctaaggaaa gcacaaaaca atggttcaga gataaattgg
     3481 ctaatttgtt taacagttgg agactgtgca atgtacgact tggaggagtt tgtttggcat
     3541 aatgattttg taggtctgtc agcattttga tatattgtgg attagccagg tgaatgatcc
     3601 ttgaaacatc tctttcaggt ctggagaaag agacagaatg cccttaaagt gtgaaataac
     3661 cttatacctg aattacagtt ttgtgatata aaataaagct gagtataaaa taaaaccaat
     3721 caaacacaga atttgattgg atttgttatt cattggtata caacaatttt tattcttatg
     3781 tgtgctttaa gggctgtgtt ttgctcttag ggtactcaat tttatgtttc cttttggcat
     3841 tctttgtgca aaaagaagca tgtggtggct ctcatttaaa tttcaaaatt attagtttta
     3901 tctttaacag attactgatt atacagaaga tataatttat tggaagaact gccaaataca
     3961 ttttgtcctt ctaaaaatat ggaaatttaa atagtagtct ggaaagttac catatgataa
     4021 tttttaattg tgtgatctag acatgcctta ggtaactgca gaaatagcaa attaaatgat
     4081 taatgagctt attagtcata gtaaacatac agattcctaa ttctatgaca gtgtacatat
     4141 atgaaaataa atgatgaaaa aagaactata atatttttaa aaatatgctt ttatttatat
     4201 ttcacataat gtaaagttct acaaaataaa gctagtaggc aagacccctc agtgtatacc
     4261 tttaaaccca taaattaatt ttatatcaat aaaataaaat actttctcta gttttggagc
     4321 ttctgtattt cctatagttt tctgactgtc cattttatcc atcttagaag tattcacatc
     4381 ccttctatgc aggatttctg catagtttta aatactttca ttgcctaagg gtttgggtgt
     4441 gtcagctttt tttgttttaa aaatatttaa cactaaaacc ttaaaataat gaagctactt
     4501 atcagaccac tgagccaaga tcctggtatt aaaagaaagt ctgggtgctt aagataaagg
     4561 gacagagtat tggtatccca gttatttggg agctttaaga ttggaacaag atatcactgt
     4621 cttgttttca cttagatcct acttacaaag tgagggttat taacagaata aagccttcct
     4681 ttaaagcttt ataataatca tatttattaa taatgctgtt gtgcatactt atagtatgca
     4741 tatattcagc atatgttgca tgtcttcaga attacataaa atgaaatccc tttcattgca
     4801 acttgcaagt gagaaaagat ccttagtggc tctggtggaa gaaatagtat ttcttcttct
     4861 cagggtgtct ccctgccttg gcccctccct gagccccagg ctttaaaagt gaaaatgttt
     4921 gaaacatgaa acatgtctgt aggaagcatc agcatggcca taagtgcagt gattttcata
     4981 tatgcctctg cccatttcaa atatattttt gacatgaata aatctaacag tatacagaat
     5041 aattcatgta agaccctaac gtgtacatgt gaaaaagcat ttctatataa tgtgaggagc
     5101 actggccatc aattagggaa ataaaggtca tgtaatattg caaattttca aaatagagcc
     5161 ctgcaagata actgcaatca taccaaaaac tatttgagta aatggatttt taaagtaatt
     5221 tttgtttaga agagtttata tttcagaagc agaaaatgtc aaatgatagt ctttgtaaat
     5281 ggtggtgcac cttctctatg cacatctgtc ttttacatca gaaagagctg tggtcatgct
     5341 aaaattagag ataacttttt gaatgacttg gtcaagctgt gtgtaaaata tttaaccata
     5401 agtcaagtac agtgtactat gtttaataaa gttacattta atgcatttat tgcatatatg
     5461 aatatataca tgaagaggct ttatgtcttc tggtatttga ttttgaatgt tttttaagtc
     5521 agtggtgcct ttaggcaaga actttcgaaa ttaatcattc tttgtgtttt ctgatttttc
     5581 aggtaacatg tacactattt agaaaccatc atagtttatt caccttaaaa aattgattgt
     5641 attatttaaa tatatcactt agatgggcat ttcctataat taggatattc caaatagttg
     5701 ctgaaatcaa ttgtgccatt gaccaatgga tgcacttggt tagccttaat ttttttaaaa
     5761 aaaaaaaaaa caaaaacttt cttgaatatt tcagtttgtt cattttaagt ttgtgctgct
     5821 ggtatttggg aagaaaaatc aatcagttaa gtgctaccag aaagtgtacc aatttttata
     5881 aaaattaaga atgatataaa tttcacaatc taaaatttta attttgtgca atattttcat
     5941 ttaatgcatg tgtatttgag taattgtaag ataaatgaat ttttaatttt ttgagatgta
     6001 gcttaaactg tcttcttaat ccaacaactt acattccgtt gttgctgcat ccttttattt
     6061 aaggacttgt tttaaaagtc tttttgtcac tccttggaaa attcttttat tagtagtaaa
     6121 ttttgctttt aggagcatgg ctcctgtatt acaagggaaa aaatatgaag agcacatttt
     6181 aggattataa ttgttaaaag ataatgtgtg catatcattg tacagtaagt gtcaactgtg
     6241 tgcctaactg ttctatcaat actttctttc tttaacaaag aagaaacaac aatcagaatg
     6301 tcagttattt ttattcaact aaaaggatta aaaaatttat ttggtttgtg ttctgtccat
     6361 tagaaaatac taataaagta ttaacaaaca
//
