LOCUS       NM_003089               1671 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens small nuclear ribonucleoprotein U1 subunit 70
            (SNRNP70), transcript variant 1, mRNA.
ACCESSION   NM_003089
VERSION     NM_003089.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Paul,T., Zhang,P., Zhang,Z., Fargason,T., De Silva,N.I.U.,
            Powell,E., Ekpenyong,E., Jamal,S., Yu,Y., Prevelige,P., Lu,R. and
            Zhang,J.
  TITLE     The U1-70K and SRSF1 interaction is modulated by phosphorylation
            during the early stages of spliceosome assembly
  JOURNAL   Protein Sci 33 (8), e5117 (2024)
   PUBMED   39023093
  REMARK    GeneRIF: The U1-70K and SRSF1 interaction is modulated by
            phosphorylation during the early stages of spliceosome assembly.
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Jiang,D., Zhu,X.L., An,Y. and Li,Y.R.
  TITLE     Clinical significance of small nuclear ribonucleoprotein U1 subunit
            70 in patients with hepatocellular carcinoma
  JOURNAL   PeerJ 12, e16876 (2024)
   PUBMED   38500533
  REMARK    GeneRIF: Clinical significance of small nuclear ribonucleoprotein
            U1 subunit 70 in patients with hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1671)
  AUTHORS   O'Neill,A.C., Uzbas,F., Antognolli,G., Merino,F., Draganova,K.,
            Jack,A., Zhang,S., Pedini,G., Schessner,J.P., Cramer,K.,
            Schepers,A., Metzger,F., Esgleas,M., Smialowski,P., Guerrini,R.,
            Falk,S., Feederle,R., Freytag,S., Wang,Z., Bahlo,M., Jungmann,R.,
            Bagni,C., Borner,G.H.H., Robertson,S.P., Hauck,S.M. and Gotz,M.
  TITLE     Spatial centrosome proteome of human neural cells uncovers
            disease-relevant heterogeneity
  JOURNAL   Science 376 (6599), eabf9088 (2022)
   PUBMED   35709258
REFERENCE   4  (bases 1 to 1671)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 1671)
  AUTHORS   Voss,P.G., Haudek,K.C., Patterson,R.J. and Wang,J.L.
  TITLE     Galectin-3-U1 snRNP Complexes Initiate Splicing Activity in
            U1-Depleted Nuclear Extracts
  JOURNAL   Methods Mol Biol 2442, 713-726 (2022)
   PUBMED   35320554
  REMARK    GeneRIF: Galectin-3-U1 snRNP Complexes Initiate Splicing Activity
            in U1-Depleted Nuclear Extracts.
REFERENCE   6  (bases 1 to 1671)
  AUTHORS   Wilk,H.E., Schaefer,K.P., Agris,P.F., Boak,A.M. and Kovacs,S.A.
  TITLE     U1 SnRNP association with HnRNP involves an initial non-specific
            splice-site independent interaction of U1 SnRNP protein with HnRNA
  JOURNAL   Mol Cell Biochem 106 (1), 55-66 (1991)
   PUBMED   1833625
REFERENCE   7  (bases 1 to 1671)
  AUTHORS   Spritz,R.A., Strunk,K., Surowy,C.S. and Mohrenweiser,H.W.
  TITLE     Human U1-70K ribonucleoprotein antigen gene: organization,
            nucleotide sequence, and mapping to locus 19q13.3
  JOURNAL   Genomics 8 (2), 371-379 (1990)
   PUBMED   2147422
REFERENCE   8  (bases 1 to 1671)
  AUTHORS   Du Clos,T.W.
  TITLE     C-reactive protein reacts with the U1 small nuclear
            ribonucleoprotein
  JOURNAL   J Immunol 143 (8), 2553-2559 (1989)
   PUBMED   2477447
REFERENCE   9  (bases 1 to 1671)
  AUTHORS   Query,C.C., Bentley,R.C. and Keene,J.D.
  TITLE     A common RNA recognition motif identified within a defined U1 RNA
            binding domain of the 70K U1 snRNP protein
  JOURNAL   Cell 57 (1), 89-101 (1989)
   PUBMED   2467746
REFERENCE   10 (bases 1 to 1671)
  AUTHORS   Spritz,R.A., Strunk,K., Surowy,C.S., Hoch,S.O., Barton,D.E. and
            Francke,U.
  TITLE     The human U1-70K snRNP protein: cDNA cloning, chromosomal
            localization, expression, alternative splicing and RNA-binding
  JOURNAL   Nucleic Acids Res 15 (24), 10373-10391 (1987)
   PUBMED   2447561
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB189176.1, BC001315.1, X06815.1 and BM969999.1.
            
            On Nov 22, 2018 this sequence version replaced NM_003089.5.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163655.196941.1,
                                           SRR3476690.182849.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000598441.6/ ENSP00000472998.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                DB189176.1         269-284
            17-1128             BC001315.1         1-1112
            1129-1669           X06815.1           1129-1669
            1670-1671           BM969999.1         27-28               c
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..1671
                     /gene="SNRNP70"
                     /gene_synonym="RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U170K;
                     U1AP; U1RNP"
                     /note="small nuclear ribonucleoprotein U1 subunit 70"
                     /db_xref="GeneID:6625"
                     /db_xref="HGNC:HGNC:11150"
                     /db_xref="MIM:180740"
     exon            1..186
                     /gene="SNRNP70"
                     /gene_synonym="RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U170K;
                     U1AP; U1RNP"
                     /inference="alignment:Splign:2.1.0"
     exon            187..343
                     /gene="SNRNP70"
                     /gene_synonym="RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U170K;
                     U1AP; U1RNP"
                     /inference="alignment:Splign:2.1.0"
     CDS             197..1510
                     /gene="SNRNP70"
                     /gene_synonym="RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U170K;
                     U1AP; U1RNP"
                     /note="isoform 1 is encoded by transcript variant 1; U1
                     small nuclear ribonucleoprotein 70 kDa; U1 snRNP 70 kDa;
                     small nuclear ribonucleoprotein 70kDa (U1); small nuclear
                     ribonucleoprotein, U1 70kDa subunit"
                     /codon_start=1
                     /product="U1 small nuclear ribonucleoprotein 70 kDa
                     isoform 1"
                     /protein_id="NP_003080.2"
                     /db_xref="CCDS:CCDS12756.1"
                     /db_xref="GeneID:6625"
                     /db_xref="HGNC:HGNC:11150"
                     /db_xref="MIM:180740"
                     /translation="MTQFLPPNLLALFAPRDPIPYLPPLEKLPHEKHHNQPYCGIAPY
                     IREFEDPRDAPPPTRAETREERMERKRREKIERRQQEVETELKMWDPHNDPNAQGDAF
                     KTLFVARVNYDTTESKLRREFEVYGPIKRIHMVYSKRSGKPRGYAFIEYEHERDMHSA
                     YKHADGKKIDGRRVLVDVERGRTVKGWRPRRLGGGLGGTRRGGADVNIRHSGRDDTSR
                     YDERPGPSPLPHRDRDRDRERERRERSRERDKERERRRSRSRDRRRRSRSRDKEERRR
                     SRERSKDKDRDRKRRSSRSRERARRERERKEELRGGGGDMAEPSEAGDAPPDDGPPGE
                     LGPDGPDGPEEKGRDRDRERRRSHRSERERRRDRDRDRDRDREHKRGERGSERGRDEA
                     RGGGGGQDNGLEGLGNDSRDMYMESEGGDGYLAPENGYLMEAAPE"
     exon            344..406
                     /gene="SNRNP70"
                     /gene_synonym="RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U170K;
                     U1AP; U1RNP"
                     /inference="alignment:Splign:2.1.0"
     exon            407..461
                     /gene="SNRNP70"
                     /gene_synonym="RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U170K;
                     U1AP; U1RNP"
                     /inference="alignment:Splign:2.1.0"
     exon            462..526
                     /gene="SNRNP70"
                     /gene_synonym="RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U170K;
                     U1AP; U1RNP"
                     /inference="alignment:Splign:2.1.0"
     exon            527..589
                     /gene="SNRNP70"
                     /gene_synonym="RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U170K;
                     U1AP; U1RNP"
                     /inference="alignment:Splign:2.1.0"
     exon            590..671
                     /gene="SNRNP70"
                     /gene_synonym="RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U170K;
                     U1AP; U1RNP"
                     /inference="alignment:Splign:2.1.0"
     exon            672..773
                     /gene="SNRNP70"
                     /gene_synonym="RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U170K;
                     U1AP; U1RNP"
                     /inference="alignment:Splign:2.1.0"
     exon            774..861
                     /gene="SNRNP70"
                     /gene_synonym="RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U170K;
                     U1AP; U1RNP"
                     /inference="alignment:Splign:2.1.0"
     exon            862..1671
                     /gene="SNRNP70"
                     /gene_synonym="RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U170K;
                     U1AP; U1RNP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1650..1655
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SNRNP70"
                     /gene_synonym="RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U170K;
                     U1AP; U1RNP"
                     /note="hexamer: AATAAA"
     polyA_site      1671
                     /gene="SNRNP70"
                     /gene_synonym="RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U170K;
                     U1AP; U1RNP"
                     /note="major polyA site"
ORIGIN      
        1 agtcgctatc ggaggccgcg cgggtggctg agcagcggcc tggtgcgctc gcttagcggg
       61 cgacggaatc agacggacgt ggacgccccc ggagtggaag ccgaagcagg agttgttgtt
      121 gctgaggggc tgccgcagcc gccgcgagcc tccggacaga cgccagagcg aggagggcgc
      181 tacgcgactt ggcaagatga cccagttcct gccgcccaac cttctggccc tctttgcccc
      241 ccgtgaccct attccatacc tgccacccct ggagaaactg ccacatgaaa aacaccacaa
      301 tcaaccttat tgtggcattg cgccgtacat tcgagagttt gaggaccctc gagatgcccc
      361 tcctccaact cgtgctgaaa cccgagagga gcgcatggag aggaaaagac gggaaaagat
      421 tgagcggcga cagcaagaag tggagacaga gcttaaaatg tgggaccctc acaatgatcc
      481 caatgctcag ggggatgcct tcaagactct cttcgtggcg agagtgaatt atgacacaac
      541 agaatccaag ctccggagag agtttgaggt gtacggacct atcaaaagaa tacacatggt
      601 ctacagtaag cggtcaggaa agccccgtgg ctatgccttc atcgagtacg aacacgagcg
      661 agacatgcac tccgcttaca aacacgcaga tggcaagaag attgatggca ggagggtcct
      721 tgtggacgtg gagaggggcc gaaccgtgaa gggctggagg ccccggcggc taggaggagg
      781 cctcggtggt accagaagag gaggggctga tgtgaacatc cggcattcag gccgcgatga
      841 cacctcccgc tacgatgaga ggcccggccc ctccccgctt ccgcacaggg accgggaccg
      901 ggaccgtgag cgggagcgca gagagcggag ccgggagcga gacaaggagc gagaacggcg
      961 acgctcccgc tcccgggacc ggcggaggcg ctcacggagt cgcgacaagg aggagcggag
     1021 gcgctccagg gagcggagca aggacaagga ccgggaccgg aagcggcgaa gcagccggag
     1081 tcgggagcgg gcccggcggg agcgggagcg caaggaggag ctgcgtggcg gcggtggcga
     1141 catggcggag ccctccgagg cgggtgacgc gccccctgat gatgggcctc caggggagct
     1201 cgggcctgac ggccctgacg gtccagagga aaagggccgg gatcgtgacc gggagcgacg
     1261 gcggagccac cggagcgagc gcgagcggcg ccgggaccgg gatcgtgacc gtgaccgtga
     1321 ccgcgagcac aaacgggggg agcggggcag tgagcggggc agggatgagg cccgaggtgg
     1381 gggcggtggc caggacaacg ggctggaggg tctgggcaac gacagccgag acatgtacat
     1441 ggagtctgag ggcggcgacg gctacctggc tccggagaat gggtatttga tggaggctgc
     1501 gccggagtga agaggtcgtc ctctccatct gctgtgtttg gacgcgttcc tgcccagccc
     1561 cttgctgtca tcccctcccc caaccttggc cacttgagtt tgtcctccaa gggtaggtgt
     1621 ctcatttgtt ctggcccctt ggatttaaaa ataaaattaa tttcctgttg a
//
