LOCUS       NM_003825               2310 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens synaptosome associated protein 23 (SNAP23), transcript
            variant 1, mRNA.
ACCESSION   NM_003825
VERSION     NM_003825.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Gigon,L., Fettrelet,T., Miholic,M., McLeish,K.R., Yousefi,S.,
            Stojkov,D. and Simon,H.U.
  TITLE     Syntaxin-4 and SNAP23 are involved in neutrophil degranulation, but
            not in the release of mitochondrial DNA during NET formation
  JOURNAL   Front Immunol 14, 1272699 (2023)
   PUBMED   37885878
  REMARK    GeneRIF: Syntaxin-4 and SNAP23 are involved in neutrophil
            degranulation, but not in the release of mitochondrial DNA during
            NET formation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Xie,B.W., Guan,B., Chen,W., Zhou,M., Gu,Q., Liu,Y. and Yan,D.
  TITLE     Tumor-derived extracellular vesicles delivering TNF-alpha promotes
            colorectal cancer metastasis via the NF-kB/LAMB3/AKT axis by
            targeting SNAP23
  JOURNAL   Arch Biochem Biophys 741, 109605 (2023)
   PUBMED   37086961
  REMARK    GeneRIF: Tumor-derived extracellular vesicles delivering TNF-alpha
            promotes colorectal cancer metastasis via the NF-kB/LAMB3/AKT axis
            by targeting SNAP23.
REFERENCE   3  (bases 1 to 2310)
  AUTHORS   Miller,K., Wagner,M.A., Jassey,A. and Jackson,W.T.
  TITLE     SNAP23 is essential for germination of EV-D68 replication
            organelles
  JOURNAL   Virology 578, 117-127 (2023)
   PUBMED   36527930
  REMARK    GeneRIF: SNAP23 is essential for germination of EV-D68 replication
            organelles.
REFERENCE   4  (bases 1 to 2310)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 2310)
  AUTHORS   Mitani,F., Hayasaka,R., Hirayama,A. and Oneyama,C.
  TITLE     SNAP23-Mediated Perturbation of Cholesterol-Enriched Membrane
            Microdomain Promotes Extracellular Vesicle Production in
            Src-Activated Cancer Cells
  JOURNAL   Biol Pharm Bull 45 (10), 1572-1580 (2022)
   PUBMED   36184518
  REMARK    GeneRIF: SNAP23-Mediated Perturbation of Cholesterol-Enriched
            Membrane Microdomain Promotes Extracellular Vesicle Production in
            Src-Activated Cancer Cells.
REFERENCE   6  (bases 1 to 2310)
  AUTHORS   Galli,T., Zahraoui,A., Vaidyanathan,V.V., Raposo,G., Tian,J.M.,
            Karin,M., Niemann,H. and Louvard,D.
  TITLE     A novel tetanus neurotoxin-insensitive vesicle-associated membrane
            protein in SNARE complexes of the apical plasma membrane of
            epithelial cells
  JOURNAL   Mol Biol Cell 9 (6), 1437-1448 (1998)
   PUBMED   9614185
REFERENCE   7  (bases 1 to 2310)
  AUTHORS   Tang,B.L., Tan,A.E., Lim,L.K., Lee,S.S., Low,D.Y. and Hong,W.
  TITLE     Syntaxin 12, a member of the syntaxin family localized to the
            endosome
  JOURNAL   J Biol Chem 273 (12), 6944-6950 (1998)
   PUBMED   9507000
REFERENCE   8  (bases 1 to 2310)
  AUTHORS   Araki,S., Tamori,Y., Kawanishi,M., Shinoda,H., Masugi,J., Mori,H.,
            Niki,T., Okazawa,H., Kubota,T. and Kasuga,M.
  TITLE     Inhibition of the binding of SNAP-23 to syntaxin 4 by Munc18c
  JOURNAL   Biochem Biophys Res Commun 234 (1), 257-262 (1997)
   PUBMED   9168999
REFERENCE   9  (bases 1 to 2310)
  AUTHORS   Mollinedo,F. and Lazo,P.A.
  TITLE     Identification of two isoforms of the vesicle-membrane fusion
            protein SNAP-23 in human neutrophils and HL-60 cells
  JOURNAL   Biochem Biophys Res Commun 231 (3), 808-812 (1997)
   PUBMED   9070898
REFERENCE   10 (bases 1 to 2310)
  AUTHORS   Ravichandran,V., Chawla,A. and Roche,P.A.
  TITLE     Identification of a novel syntaxin- and synaptobrevin/VAMP-binding
            protein, SNAP-23, expressed in non-neuronal tissues
  JOURNAL   J Biol Chem 271 (23), 13300-13303 (1996)
   PUBMED   8663154
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK290152.1, BC022890.1 and
            BM782600.1.
            
            On Nov 22, 2018 this sequence version replaced NM_003825.3.
            
            Summary: Specificity of vesicular transport is regulated, in part,
            by the interaction of a vesicle-associated membrane protein termed
            synaptobrevin/VAMP with a target compartment membrane protein
            termed syntaxin. These proteins, together with SNAP25
            (synaptosome-associated protein of 25 kDa), form a complex which
            serves as a binding site for the general membrane fusion machinery.
            Synaptobrevin/VAMP and syntaxin are believed to be involved in
            vesicular transport in most, if not all cells, while SNAP25 is
            present almost exclusively in the brain, suggesting that a
            ubiquitously expressed homolog of SNAP25 exists to facilitate
            transport vesicle/target membrane fusion in other tissues. The
            protein encoded by this gene is structurally and functionally
            similar to SNAP25 and binds tightly to multiple syntaxins and
            synaptobrevins/VAMPs. It is an essential component of the high
            affinity receptor for the general membrane fusion machinery and is
            an important regulator of transport vesicle docking and fusion. Two
            alternative transcript variants encoding different protein isoforms
            have been described for this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) is the longer transcript,
            containing eight exons and encoding the full-length isoform
            (SNAP23A).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189658.112212.1,
                                           SRR1660809.125591.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000249647.8/ ENSP00000249647.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1058              AK290152.1         375-1432
            1059-2308           BC022890.1         1044-2293
            2309-2310           BM782600.1         370-371
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.1-q15.2"
     gene            1..2310
                     /gene="SNAP23"
                     /gene_synonym="HsT17016; SNAP-23; SNAP23A; SNAP23B"
                     /note="synaptosome associated protein 23"
                     /db_xref="GeneID:8773"
                     /db_xref="HGNC:HGNC:11131"
                     /db_xref="MIM:602534"
     exon            1..80
                     /gene="SNAP23"
                     /gene_synonym="HsT17016; SNAP-23; SNAP23A; SNAP23B"
                     /inference="alignment:Splign:2.1.0"
     exon            81..151
                     /gene="SNAP23"
                     /gene_synonym="HsT17016; SNAP-23; SNAP23A; SNAP23B"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..730
                     /gene="SNAP23"
                     /gene_synonym="HsT17016; SNAP-23; SNAP23A; SNAP23B"
                     /note="isoform SNAP23A is encoded by transcript variant 1;
                     synaptosomal-associated protein 23;
                     synaptosomal-associated protein, 23kDa; vesicle-membrane
                     fusion protein SNAP-23; synaptosome associated protein
                     23kDa"
                     /codon_start=1
                     /product="synaptosomal-associated protein 23 isoform
                     SNAP23A"
                     /protein_id="NP_003816.2"
                     /db_xref="CCDS:CCDS10087.1"
                     /db_xref="GeneID:8773"
                     /db_xref="HGNC:HGNC:11131"
                     /db_xref="MIM:602534"
                     /translation="MDNLSSEEIQQRAHQITDESLESTRRILGLAIESQDAGIKTITM
                     LDEQKEQLNRIEEGLDQINKDMRETEKTLTELNKCCGLCVCPCNRTKNFESGKAYKTT
                     WGDGGENSPCNVVSKQPGPVTNGQLQQPTTGAASGGYIKRITNDAREDEMEENLTQVG
                     SILGNLKDMALNIGNEIDAQNPQIKRITDKADTNRDRIDIANARAKKLIDS"
     exon            152..193
                     /gene="SNAP23"
                     /gene_synonym="HsT17016; SNAP-23; SNAP23A; SNAP23B"
                     /inference="alignment:Splign:2.1.0"
     exon            194..242
                     /gene="SNAP23"
                     /gene_synonym="HsT17016; SNAP-23; SNAP23A; SNAP23B"
                     /inference="alignment:Splign:2.1.0"
     exon            243..360
                     /gene="SNAP23"
                     /gene_synonym="HsT17016; SNAP-23; SNAP23A; SNAP23B"
                     /inference="alignment:Splign:2.1.0"
     exon            361..519
                     /gene="SNAP23"
                     /gene_synonym="HsT17016; SNAP-23; SNAP23A; SNAP23B"
                     /inference="alignment:Splign:2.1.0"
     exon            520..664
                     /gene="SNAP23"
                     /gene_synonym="HsT17016; SNAP-23; SNAP23A; SNAP23B"
                     /inference="alignment:Splign:2.1.0"
     exon            665..2310
                     /gene="SNAP23"
                     /gene_synonym="HsT17016; SNAP-23; SNAP23A; SNAP23B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2060..2065
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SNAP23"
                     /gene_synonym="HsT17016; SNAP-23; SNAP23A; SNAP23B"
                     /note="hexamer: ACTAAA"
     polyA_site      2080
                     /gene="SNAP23"
                     /gene_synonym="HsT17016; SNAP-23; SNAP23A; SNAP23B"
                     /note="major polyA site"
     regulatory      2292..2297
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SNAP23"
                     /gene_synonym="HsT17016; SNAP-23; SNAP23A; SNAP23B"
                     /note="hexamer: ATTAAA"
     polyA_site      2310
                     /gene="SNAP23"
                     /gene_synonym="HsT17016; SNAP-23; SNAP23A; SNAP23B"
                     /note="major polyA site"
ORIGIN      
        1 aggcgcgact agggtgcagc gccaggtccg gtgttggggt gtccgagttg ccgccggaga
       61 ggagtggcct cgcccgcttg agttttgatt catcatggat aatctgtcat cagaagaaat
      121 tcaacagaga gctcaccaga ttactgatga gtctctggaa agtacgagga gaatcctggg
      181 tttagccatt gagtctcagg atgcaggaat caagaccatc actatgctgg atgaacaaaa
      241 ggaacaacta aaccgcatag aagaaggctt ggaccaaata aataaggaca tgagagagac
      301 agagaagact ttaacagaac tcaacaaatg ctgtggcctt tgtgtctgcc catgtaatag
      361 aacaaagaac tttgagtctg gcaaggctta taagacaaca tggggagatg gtggagaaaa
      421 ctcaccttgc aatgtagtat ctaaacagcc aggcccggtg acaaatggtc agcttcagca
      481 accaacaacg ggagcagcca gtggtggata cattaaacgc ataactaatg atgccagaga
      541 agatgaaatg gaagagaacc tgactcaagt gggcagtatc ctgggaaatc taaaagacat
      601 ggccctgaac ataggcaatg agattgatgc tcaaaatcca caaataaaac gaatcacaga
      661 caaggctgac accaacagag atcgtattga tattgccaat gccagagcaa agaaactcat
      721 tgacagctaa agctactgct gttcttcttt atcatttatt cacttccgta gctcctcctt
      781 gaaagttatt accttttcag agtttaagtt ttcggttcca cgctcttcta attgggagat
      841 aatatggaag aagggccaga gcagttacag ccctccttct tttttgtttt ctgttgaggg
      901 ccgactgctg ctctgccttc cttctagtat tttctttctc aattcatacg cttagattgg
      961 ttttcatatg tcatgtttag tgttttcatc ctcctcatat acttcagcag gttcttttgc
     1021 tttcaagatt tggaagcatt gccaaagaca gccatgaaga aggaagctgt agaggtgttt
     1081 tttgttgttg tttatttttg cttttgtggt tgagggaagg acaagagata agaggttgtt
     1141 acctcagtaa aaaccttcag gccacaaagc aaaaagttgc atagccacaa cgaagatcta
     1201 gttggatata gtttttgatt taagttgcag ttatagccaa tttaggctaa tgcttggttt
     1261 tggagctttt atacacaacg tttttgttag gcatcacagt tttgcaacct ctgctccaaa
     1321 gagaaaaata gaatgagttt tctttctttt tttttttttt ggagtcagag tctcgctctc
     1381 ttgtccaggc tggagtgcaa tagcgcgatc tgggctcact gcaacctccg cctccctggt
     1441 tcaagcaatt ctcctgcctt gcctcctgag tagctgggat tacaggcgtg tgtcaccacg
     1501 ctcggctaat ttttgtattt ttagtagaga cagggtttca ccatgtcaag ctggtcttgg
     1561 actcctgacg tcgtgatcca cccgccttgg cctcccaaag tactgggatt acaggcgtga
     1621 gccactgtgc ctggctgagt tttcttaaag tgagcttaat ttctgaactc ttagtgattc
     1681 atatatgtac ataaatctgt gatcccattt cttattgcac cattcaggaa cactttatat
     1741 aaatgagtgg ctttttattt catattatta gtagtatcat ggttccatta caggcctatt
     1801 aacatcatac attgtcatta gtctttgaag aaaaaatatg taaatatata tgtgtaacat
     1861 gagaatttct ctctaaagca gggcttaaaa ttttttggaa aagtttgaca aagcatacca
     1921 catgaattca gatttacctc aatgctaaga attatgttta gttaggaaaa aggaaagtca
     1981 ttttgacctc aggtagaaaa atagattgct ttgagtttta tgtagcttta gactttaaaa
     2041 agttagaatt tattctgtaa ctaaaaatta tttgaaaaaa ttatgcctct ggtttaatta
     2101 ttggtgatta cacactcttt ctcttaccct tgtgtattga actatgtcca taatcaagtt
     2161 gatgtggatc ctgaaaagtg ttatgaacat ctgattggta tttgtcacat ttattttaaa
     2221 attagcatct gaacacttca aagctgtcag tgtgtattgg tttcaccaat aaccactgct
     2281 tgatccttac aattaaattt tttaactaac
//
