LOCUS       NM_174921               6252 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens small integral membrane protein 14 (SMIM14),
            transcript variant 2, mRNA.
ACCESSION   NM_174921
VERSION     NM_174921.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 6252)
  AUTHORS   Jun,M.H., Jun,Y.W., Kim,K.H., Lee,J.A. and Jang,D.J.
  TITLE     Characterization of the cellular localization of C4orf34 as a novel
            endoplasmic reticulum resident protein
  JOURNAL   BMB Rep 47 (10), 563-568 (2014)
   PUBMED   24499674
  REMARK    GeneRIF: hC4orf34 is an endoplasmic reticulum-resident type I
            transmembrane protein.
REFERENCE   4  (bases 1 to 6252)
  AUTHORS   Nain,A.S. and Cimini,D.
  TITLE     MISP: The missing link between extracellular matrix and astral
            microtubules
  JOURNAL   Cell Cycle 12 (12), 1821-1822 (2013)
   PUBMED   23759569
REFERENCE   5  (bases 1 to 6252)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
REFERENCE   6  (bases 1 to 6252)
  AUTHORS   Lehner,B. and Sanderson,C.M.
  TITLE     A protein interaction framework for human mRNA degradation
  JOURNAL   Genome Res 14 (7), 1315-1323 (2004)
   PUBMED   15231747
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            HY155982.1, BC008502.1, BE615527.1, AC021148.8 and AA480975.1.
            
            On Nov 23, 2018 this sequence version replaced NM_174921.2.
            
            Transcript Variant: This variant (2) lacks an alternate exon in the
            5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same
            protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.3642976.1,
                                           SRR18074968.2046017.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000295958.10/ ENSP00000295958.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 HY155982.1         135-138
            5-1407              BC008502.1         1-1403
            1408-1408           BE615527.1         458-458
            1409-1658           BC008502.1         1405-1654
            1659-5272           AC021148.8         113133-116746       c
            5273-5291           AA480975.1         18-36               c
            5292-6252           AC021148.8         112153-113113       c
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p14"
     gene            1..6252
                     /gene="SMIM14"
                     /gene_synonym="C4orf34"
                     /note="small integral membrane protein 14"
                     /db_xref="GeneID:201895"
                     /db_xref="HGNC:HGNC:27321"
     exon            1..127
                     /gene="SMIM14"
                     /gene_synonym="C4orf34"
                     /inference="alignment:Splign:2.1.0"
     exon            128..237
                     /gene="SMIM14"
                     /gene_synonym="C4orf34"
                     /inference="alignment:Splign:2.1.0"
     CDS             163..462
                     /gene="SMIM14"
                     /gene_synonym="C4orf34"
                     /codon_start=1
                     /product="small integral membrane protein 14"
                     /protein_id="NP_777581.1"
                     /db_xref="CCDS:CCDS3456.1"
                     /db_xref="GeneID:201895"
                     /db_xref="HGNC:HGNC:27321"
                     /translation="MAEGGFDPCECVCSHEHAMRRLINLLRQSQSYCTDTECLQELPG
                     PSGDNGISVTMILVAWMVIALILFLLRPPNLRGSSLPGKPTSPHNGQDPPAPPVD"
     exon            238..286
                     /gene="SMIM14"
                     /gene_synonym="C4orf34"
                     /inference="alignment:Splign:2.1.0"
     exon            287..429
                     /gene="SMIM14"
                     /gene_synonym="C4orf34"
                     /inference="alignment:Splign:2.1.0"
     exon            430..6252
                     /gene="SMIM14"
                     /gene_synonym="C4orf34"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5272..5277
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SMIM14"
                     /gene_synonym="C4orf34"
                     /note="hexamer: AATAGA"
     polyA_site      5291
                     /gene="SMIM14"
                     /gene_synonym="C4orf34"
                     /note="major polyA site"
     regulatory      5992..5997
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SMIM14"
                     /gene_synonym="C4orf34"
                     /note="hexamer: AATAAA"
     polyA_site      6018
                     /gene="SMIM14"
                     /gene_synonym="C4orf34"
     regulatory      6235..6240
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SMIM14"
                     /gene_synonym="C4orf34"
                     /note="hexamer: AATAAA"
     polyA_site      6252
                     /gene="SMIM14"
                     /gene_synonym="C4orf34"
ORIGIN      
        1 ggccagaaac ggcagtggca gcagcgtccg gagcagccgc agccttctgg aagctccagg
       61 cggtctttct gccgagcctc ggtcccggcc cccatcctcc ccgccccatc ggttgttgtc
      121 tgggcggatt taaacagtca agtaaaatca agctgggtaa tcatggcaga aggtggattt
      181 gatccctgtg aatgtgtttg ctctcatgaa catgcaatga gaagactgat caatctgtta
      241 cggcagtccc agtcctactg cacagacaca gagtgtcttc aggaattacc gggaccctct
      301 ggtgataatg gcatcagtgt tacaatgatc ttggtagcct ggatggttat tgcattgatc
      361 ttgttcttac tgagacctcc taatctaaga ggatccagcc tacctggaaa gccaaccagt
      421 cctcataatg gacaagatcc accagctcct cctgtggact aactttgtga tatgggaagt
      481 gaaaatagtt aacaccttgc acgaccaaac gaacgaagat gaccagagta ctcttaaccc
      541 cattagaact gtttttcctt ttgtatctgc aatatgggat ggtattgttt tcatgagctt
      601 ctagaaattt cacttgcaag tttatttttg cttcctgtgt tactgccatt cctatttaca
      661 gtatatttga gtgaatgatt atatttttaa aaagttacat ggggcttttt tggttgtcct
      721 aaacttacaa acattccact cattctgttt gtaactgtga ttataatttt tgtgataatt
      781 tctggcctga ttgaaggaaa tttgagaggt ctgcatttat atattttaaa tagatttgat
      841 aggtttttaa attgcttttt ttcataaggt atttataaag ttatttgggg ttgtctggga
      901 ttgtgtgaaa gaaaattaga accacgctgt atttacattt accttggtag tttatttgtg
      961 gatggcagtt ttctgtagtt ttggggactg tggtagctct tggattgttt tgcaaattac
     1021 agctgaaatc tgtgtcatgg attaaactgg cttatgtggc tagaatagga agagagaaaa
     1081 aatgaaatgg ttgtttacta attttatact cccattaaaa atctctaatg ttaagaaaac
     1141 cttaaataaa catgattgat caatatggca gatgatttac agtccattat atgacatttg
     1201 taagccactc aacactagcc ttacaaggtt catgaaagga ggaaaacatt aattctttct
     1261 gcctttgctg atataatcca catagtaata atttaagcta taatttattt ttaaaatgag
     1321 tgcctcttgg gaagcttctt ttctgttcta acagttccct gttaaaatga aaatcttagg
     1381 ccagtgcagt ggctcacacc tgtaatccca gcactttggg aggctgaggt gggtggatca
     1441 cttgaggtca ggagttcgag accagcctgg ccaacatggt gaaaccctgt ctctactaaa
     1501 aatacagaaa aattagccag ggttggtgca tgtgcctgta atcccagcta ctcaggaggc
     1561 caaagcagga gaattgcttg aacccgggag gtggaggttg cagtgaacca agatcgcacc
     1621 actgcattcc agcctaagca atagagcgag actccctctc aaaaaaaaaa aaagaagaaa
     1681 atcttaaagc tgtattaggg cctgttaggt tactgatatt tgaatgccta tttacttatt
     1741 tactacattg agtcttttgt tatcctctga cctataaaat attttaataa attactggtt
     1801 tacttcaagg gaaaagaagg aaaatgagaa acatacatct ttttttcact ccttatctca
     1861 ttcctgtttg gtgatgggat ggattggggt aggaaggaat gttaatatga gagaaatttt
     1921 aaaatgtata aaaaagtaaa aggttgtatt gttttgctca gtttgaaata aagtgaatac
     1981 caatagcact taaaaatgtc tgcatgggct gggcacggtg gctcacgcct gtaatcccag
     2041 cactttggga ggccgaggca ggcgaatcac ctgaggtcag gagttcgaga ccagcctgac
     2101 caacatggtg aaaccttatc tctactaaaa atgcaaaaat tagccaggca tggtggtgtg
     2161 tgcctataat cccagctact ggggaagctg aggcaggaaa ttgcttgaac ctgggagacg
     2221 gaggttgcag taagctgaga tcgtgccact gcactccaat cagggcgaca gagcgagact
     2281 ctgtctcaaa ataaataaat aaataaaaat gtctgcatga cccatatggt tatgaagttt
     2341 ttttcctagc aaatgattgc agattattta tgggggctag aagatatttt gctatcaatg
     2401 taaggatttt tttcccctta ggtcagtcta ttcactgttt agatctggta aattacttgt
     2461 atgaattgaa taaattctct gtatcattgt actgtttaat agtctctgat acaattgtga
     2521 tactcataag gtattttccc ccctttatta tgattacatt ctagatcata aacattgtaa
     2581 gggcatcttt tcattatata atccaatatt gattgtttga attcctcctt gtacatcctg
     2641 gctttgtgct caatttcttg aaataatata accaactcta aattacttgt gttaatagat
     2701 cggtaacatg acacaggtaa gttaaatcct ggataaatga gccccacaga ataaaggttt
     2761 ttcctcttgc tgtggcctac tggaagcctt cctaactcta tagctggtaa agtagagcag
     2821 aaagggccag aatggcttaa gatggagcta aatccctata gcattccatt ttctgctctt
     2881 gcctcttgaa cttagaaggc ttgcccatat atcctaacaa tctgttctct gagctgttag
     2941 gccaatccct ggcaagagat attgatgtag tcactgaatc tatggctcac aaataattga
     3001 aaattggctt tgggagctgg gcgcagtgac tcatgcctgt aatcccagca ctttgggagg
     3061 ctggggcagg tggatcactt gaggtcagga ggttgagacc agcctggcca agacggtgaa
     3121 accccgtgcc tacaaaaaat tagccgggtt tggtgacacg gcctgtagtc ttagctactc
     3181 gggaggctga ggcaggagaa tcgcttgagc ctgggagaca ggttgcagta agccaagatc
     3241 acgccactgc actccagctt gagtgacaga gtgagaccct gtctcaaaaa aaaaaggctt
     3301 tagaaggctt tattggatga aatggctgaa acttggatac agaatgtcta gtattagaca
     3361 cctaaaaatc tggattcttt ttggttgttg ttttgagatg gagttttgct ctttcgccca
     3421 agctggagtg aagtggcgcg accttggctc actgcaacat ccacccccag gttcgagcga
     3481 ttctccttcc tcagcctcct gagtagctgg gattataagt gcccaccacc acgcccagct
     3541 aatttttgta tctttagtag agatggggtt tcgccatgta ggccaggctg gtctcaaact
     3601 cctgacctca ggtgatccac ccactatggc ctcccaaagt gctaggatta cagatgtgag
     3661 ccaccgtgcc tggccagaaa atctggattc ttattcctag ttcttcattt ctgtcacatg
     3721 cacttagttg acattacatc tacatatatt agctttttcc tacatgagcc atctatttac
     3781 ttagtaacca gtgttcttaa tgaagtattt agtcttgggt ttcttgtaaa atttctctgc
     3841 attccttaga cagtgtacta tacatgaaat attcttgttg acctagtaat ttatattatt
     3901 ccatttaatt cttaaaccta tggccttttt attgagcaca ctcttaaatc attatttggc
     3961 ttgtaaacat tcatctgaag tgtggctaca atcctcttta aataatctag gaaaaaagaa
     4021 agataaagct tacattttca cagttttggc tcttaaacac attccacaaa tgccattaag
     4081 aatttatttt gttttaggcc agtcatggtg gctcatgcct gtaatcccag caatttggga
     4141 ggctgaggca agaactgctt gagcccagga gtttgagact agcctgggca acatagcaag
     4201 accctgtctc taccaaaaaa aaaaaagttt attttgtttt agagtcattt aatgtgtttt
     4261 tatgcacaat aatagtggga ggttgttttg ttgcatttgt ttgtttgttt tgttttgttt
     4321 tgttttgctt tccatgtggg aaaagttaac attggaactg tttctagtaa aagatttttt
     4381 tcaggctggg cacggtggct catgcctgta atcccaacac tttgggagac cgagggagct
     4441 ggatcacctg aggtcaggag ttcgagacca gcctggccaa catggaaaaa ctccatctcc
     4501 actaaaaata caaaagtagc ctggtgtggt ggcacatgcc tgtaatccca gctacttggg
     4561 aagctgaggc aggagaatca cttgaacctg ggaggtggag gttgcagtga gccaagatca
     4621 cgccattgca ctccagcctg ggcaacaaga gtgaaactcc gtctcaaaaa aaaaaaaaaa
     4681 aagatgtttt tcattttttt catgttatct atccaagcac tgttccatgg tcagcaagtc
     4741 atatttcata atgtggattt tccaaaataa ttattgaata cagctattct atggctactt
     4801 ttagtgtttt tgtggtatgt ggtgtgggag tgtttatgga attaccagta tcttaaattt
     4861 tcaaaggaac cttggaagtc tatcactcta aatgaaagtc tgtcactcta catgaattat
     4921 gtgctcaaat ttgaccaact cagtttaaga cacaaaacag taatttgaag aaggaaaaat
     4981 gaagagagtt tctagtttaa tgggttaaat ttttgttgtt gcaatagtaa gtttagtctt
     5041 cttataatat ttctaaatga aaaatcatag gtatttgtta ccatgtgtga agattagttt
     5101 gttaaaagca aaagtggtcg tgtgatatgc taaatgttaa ttactgattt tatatgttta
     5161 aatcacgcca aacaaattat gtctgtgcca tccagggtct gttgttaatc tttttctgag
     5221 tacttggatt gggataaagg gcttgtacta tgcacttttt attaatgaat aaatagaaaa
     5281 cgttagtaac actttgtgtt ttctgtttgg cttttgtggg aagagaagca agcatctttt
     5341 gcctagtaga tgttaacatt gtgtattaaa cagtttcttt gtaagaccta actaaagaca
     5401 ttcctaagag agaacttaag ttttaagaaa agttgtgaga aaaatattca taaaatatgc
     5461 agtatggggc cagtattcag aagtagagtt tctctcttgg aaattagatt gcagtcacat
     5521 ttctttggtt attttctttc tctctctctt cctagaatac caggtaaaat agacatttac
     5581 tttgagggac taatatccaa tagtgttaat tatttaagca gatatatcta gctcaaaagg
     5641 aaccagacat gtcactgtac caaaaacaca caaaagtgaa aatttgcttc tgttctgtga
     5701 cctagaatgt gtccagacat taaaggtcat gaatactcat atacatgtaa aaatagtaac
     5761 actgctctgg catgattttg acgtcagtgt ttatgtaata acttcagtgt tatatttaaa
     5821 atagataaat ttgtgaaata acaatttctt ggagacagtc aactttttat gatttaatct
     5881 aaagattgtc atgtacatgt caacggatat ctgaatcctc agtgaaactg ttaagttttc
     5941 attaaatgac tctgctgcaa tactagtttt cttctcagaa aatggaattc aaataaaata
     6001 agttttttgg tcttggcatt tcacctgatg actttctctg ttctggatct caatttgtag
     6061 agttgttggg ggttcccccc cctccaaaag atgctccata aacaccaact acaattatct
     6121 ttcatgatca tatttttaaa aattggagtt acctgtgtga tcttttccca ttagttgctt
     6181 gtaggggact ttagtgtgcg tatctcttct cagacaaatg tattatgttc gttcaataaa
     6241 cacttcaaca aa
//
