LOCUS       NM_144775               8297 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens SMCR8-C9orf72 complex subunit (SMCR8), mRNA.
ACCESSION   NM_144775
VERSION     NM_144775.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Tang,D., Zheng,K., Zhu,J., Jin,X., Bao,H., Jiang,L., Li,H.,
            Wang,Y., Lu,Y., Liu,J., Liu,H., Tang,C., Feng,S., Dong,X., Xu,L.,
            Yin,Y., Dang,S., Wei,X., Ren,H., Dong,B., Dai,L., Cheng,W., Wan,M.,
            Li,Z., Chen,J., Li,H., Kong,E., Wang,K., Lu,K. and Qi,S.
  TITLE     ALS-linked C9orf72-SMCR8 complex is a negative regulator of primary
            ciliogenesis
  JOURNAL   Proc Natl Acad Sci U S A 120 (50), e2220496120 (2023)
   PUBMED   38064514
  REMARK    GeneRIF: ALS-linked C9orf72-SMCR8 complex is a negative regulator
            of primary ciliogenesis.
REFERENCE   2  (bases 1 to 8297)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 8297)
  AUTHORS   Su,M.Y., Fromm,S.A., Zoncu,R. and Hurley,J.H.
  TITLE     Structure of the C9orf72 ARF GAP complex that is haploinsufficient
            in ALS and FTD
  JOURNAL   Nature 585 (7824), 251-255 (2020)
   PUBMED   32848248
  REMARK    GeneRIF: Structure of the C9orf72 ARF GAP complex that is
            haploinsufficient in ALS and FTD.
REFERENCE   4  (bases 1 to 8297)
  AUTHORS   Goodier,J.L., Soares,A.O., Pereira,G.C., DeVine,L.R., Sanchez,L.,
            Cole,R.N. and Garcia-Perez,J.L.
  TITLE     C9orf72-associated SMCR8 protein binds in the ubiquitin pathway and
            with proteins linked with neurological disease
  JOURNAL   Acta Neuropathol Commun 8 (1), 110 (2020)
   PUBMED   32678027
  REMARK    GeneRIF: C9orf72-associated SMCR8 protein binds in the ubiquitin
            pathway and with proteins linked with neurological disease.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8297)
  AUTHORS   Tang,D., Sheng,J., Xu,L., Zhan,X., Liu,J., Jiang,H., Shu,X.,
            Liu,X., Zhang,T., Jiang,L., Zhou,C., Li,W., Cheng,W., Li,Z.,
            Wang,K., Lu,K., Yan,C. and Qi,S.
  TITLE     Cryo-EM structure of C9ORF72-SMCR8-WDR41 reveals the role as a GAP
            for Rab8a and Rab11a
  JOURNAL   Proc Natl Acad Sci U S A 117 (18), 9876-9883 (2020)
   PUBMED   32303654
  REMARK    GeneRIF: Our study not only illustrates the basis of
            C9ORF72-SMCR8-WDR41 complex assembly but also reveals the GAP
            activity of the C9ORF72-SMCR8 complex
REFERENCE   6  (bases 1 to 8297)
  AUTHORS   Sellier,C., Campanari,M.L., Julie Corbier,C., Gaucherot,A.,
            Kolb-Cheynel,I., Oulad-Abdelghani,M., Ruffenach,F., Page,A.,
            Ciura,S., Kabashi,E. and Charlet-Berguerand,N.
  TITLE     Loss of C9ORF72 impairs autophagy and synergizes with polyQ
            Ataxin-2 to induce motor neuron dysfunction and cell death
  JOURNAL   EMBO J 35 (12), 1276-1297 (2016)
   PUBMED   27103069
REFERENCE   7  (bases 1 to 8297)
  AUTHORS   Sullivan,P.M., Zhou,X., Robins,A.M., Paushter,D.H., Kim,D.,
            Smolka,M.B. and Hu,F.
  TITLE     The ALS/FTLD associated protein C9orf72 associates with SMCR8 and
            WDR41 to regulate the autophagy-lysosome pathway
  JOURNAL   Acta Neuropathol Commun 4 (1), 51 (2016)
   PUBMED   27193190
  REMARK    GeneRIF: Study describes the identification of two binding partners
            for C9orf72: SMCR8 and WDR41 and demonstrated that
            C9orf72/SMCR8/WDR41 interacts with the FIP200/Ulk1/ATG13/ATG101
            complex.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 8297)
  AUTHORS   Yachie,N., Petsalaki,E., Mellor,J.C., Weile,J., Jacob,Y., Verby,M.,
            Ozturk,S.B., Li,S., Cote,A.G., Mosca,R., Knapp,J.J., Ko,M., Yu,A.,
            Gebbia,M., Sahni,N., Yi,S., Tyagi,T., Sheykhkarimli,D., Roth,J.F.,
            Wong,C., Musa,L., Snider,J., Liu,Y.C., Yu,H., Braun,P.,
            Stagljar,I., Hao,T., Calderwood,M.A., Pelletier,L., Aloy,P.,
            Hill,D.E., Vidal,M. and Roth,F.P.
  TITLE     Pooled-matrix protein interaction screens using Barcode Fusion
            Genetics
  JOURNAL   Mol Syst Biol 12 (4), 863 (2016)
   PUBMED   27107012
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 8297)
  AUTHORS   Zhang,D., Iyer,L.M., He,F. and Aravind,L.
  TITLE     Discovery of Novel DENN Proteins: Implications for the Evolution of
            Eukaryotic Intracellular Membrane Structures and Human Disease
  JOURNAL   Front Genet 3, 283 (2012)
   PUBMED   23248642
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 8297)
  AUTHORS   Bi,W., Yan,J., Stankiewicz,P., Park,S.S., Walz,K., Boerkoel,C.F.,
            Potocki,L., Shaffer,L.G., Devriendt,K., Nowaczyk,M.J., Inoue,K. and
            Lupski,J.R.
  TITLE     Genes in a refined Smith-Magenis syndrome critical deletion
            interval on chromosome 17p11.2 and the syntenic region of the mouse
  JOURNAL   Genome Res 12 (5), 713-728 (2002)
   PUBMED   11997338
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP280386.1, AF467440.1, BC101116.2, AC127537.8 and CK724925.1.
            
            On Nov 22, 2018 this sequence version replaced NM_144775.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.99884.1, BC142680.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000406438.5/ ENSP00000385025.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-109               BP280386.1         14-122
            110-2528            AF467440.1         1-2419
            2529-3006           BC101116.2         2416-2893
            3007-3311           AF467440.1         2902-3206
            3312-7691           AC127537.8         97310-101689
            7692-8297           CK724925.1         18-623              c
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p11.2"
     gene            1..8297
                     /gene="SMCR8"
                     /gene_synonym="DENND8A"
                     /note="SMCR8-C9orf72 complex subunit"
                     /db_xref="GeneID:140775"
                     /db_xref="HGNC:HGNC:17921"
                     /db_xref="MIM:617074"
     exon            1..2857
                     /gene="SMCR8"
                     /gene_synonym="DENND8A"
                     /inference="alignment:Splign:2.1.0"
     CDS             498..3311
                     /gene="SMCR8"
                     /gene_synonym="DENND8A"
                     /note="smith-Magenis syndrome chromosomal region candidate
                     gene 8 protein; Smith-Magenis syndrome chromosome region,
                     candidate 8"
                     /codon_start=1
                     /product="guanine nucleotide exchange protein SMCR8"
                     /protein_id="NP_658988.2"
                     /db_xref="CCDS:CCDS11195.2"
                     /db_xref="GeneID:140775"
                     /db_xref="HGNC:HGNC:17921"
                     /db_xref="MIM:617074"
                     /translation="MISAPDVVAFTKEEEYEEEPYNEPALPEEYSVPLFPFASQGANP
                     WSKLSGAKFSRDFILISEFSEQVGPQPLLTIPNDTKVFGTFDLNYFSLRIMSVDYQAS
                     FVGHPPGSAYPKLNFVEDSKVVLGDSKEGAFAYVHHLTLYDLEARGFVRPFCMAYISA
                     DQHKIMQQFQELSAEFSRASECLKTGNRKAFAGELEKKLKDLDYTRTVLHTETEIQKK
                     ANDKGFYSSQAIEKANELASVEKSIIEHQDLLKQIRSYPHRKLKGHDLCPGEMEHIQD
                     QASQASTTSNPDESADTDLYTCRPAYTPKLIKAKSTKCFDKKLKTLEELCDTEYFTQT
                     LAQLSHIEHMFRGDLCYLLTSQIDRALLKQQHITNFLFEDFVEVDDRMVEKQESIPSK
                     PSQDRPPSSSLEECPIPKVLISVGSYKSSVESVLIKMEQELGDEEYKEVEVTELSSFD
                     PQENLDYLDMDMKGSISSGESIEVLGTEKSTSVLSKSDSQASLTVPLSPQVVRSKAVS
                     HRTISEDSIEVLSTCPSEALIPDDFKASYPSAINEEESYPDGNEGAIRFQASISPPEL
                     GETEEGSIENTPSQIDSSCCIGKESDGQLVLPSTPAHTHSDEDGVVSSPPQRHRQKDQ
                     GFRVDFSVENANPSSRDNSCEGFPAYELDPSHLLASRDISKTSLDNYSDTTSYVSSVA
                     STSSDRIPSAYPAGLSSDRHKKRAGQNALKFIRQYPFAHPAIYSLLSGRTLVVLGEDE
                     AIVRKLVTALAIFVPSYGCYAKPVKHWASSPLHIMDFQKWKLIGLQRVASPAGAGTLH
                     ALSRYSRYTSILDLDNKTLRCPLYRGTLVPRLADHRTQIKRGSTYYLHVQSMLTQLCS
                     KAFLYTFCHHLHLPTHDKETEELVASRQMSFLKLTLGLVNEDVRVVQYLAELLKLHYM
                     QESPGTSHPMLRFDYVPSFLYKI"
     exon            2858..8297
                     /gene="SMCR8"
                     /gene_synonym="DENND8A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8274..8279
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SMCR8"
                     /gene_synonym="DENND8A"
                     /note="hexamer: AATAAA"
     polyA_site      8297
                     /gene="SMCR8"
                     /gene_synonym="DENND8A"
                     /note="major polyA site"
ORIGIN      
        1 atgtgatgtc aagatggcgg cgccgcggta gcaggatccg ggttgtggcg tcctaggagc
       61 cgcgacggtt tctgccctcg ggcagtgagg ggcagcagcg cttggctgac ccctgcggcg
      121 tcccgtagcc tcgcgttaag ccttgttcag agcgggggct tttgctgtcg cagtgggttc
      181 cggagagtgc aggtgatttc gcagcaggtt tcttgtgctg gccgcgctcg ccggacgaag
      241 aagagaaggg cagttgggcc tgcggccttg gcgttcatga ggcctcagag agctccggag
      301 gagctacaac tgtgaggggg ctgctagagt tcttctcctc gggtgtgtga gaagcagcct
      361 aggaccccgg gttcggggag tcgcaaagaa ggccgtaagg gcttcacttc cttctccgga
      421 ggcctgcctt ctgcgcgtcg attaacaccg cattctttcc cacttcctct caatgttttc
      481 ttcatatatc tggaaatatg atcagcgccc ctgacgtagt ggccttcacc aaagaggaag
      541 agtatgaaga agagccttac aatgagccgg ccctgcctga ggagtactcg gtgccgctct
      601 tccccttcgc cagtcagggt gctaacccct ggtcaaaact gtccggggcc aagttttcga
      661 gggacttcat tcttatttcc gagttctctg agcaggtggg accccaaccc ttactgacca
      721 tccccaatga caccaaagtt tttggcactt ttgatctcaa ttacttctcc ctgcgtatca
      781 tgtctgtgga ttaccaggct tccttcgtgg gccatcctcc tggatctgcc taccccaagc
      841 tgaacttcgt ggaggactcc aaggtggtgc tgggagattc taaggagggc gcctttgcat
      901 acgtgcacca ccttacccta tacgacctgg aggcccgtgg cttcgtgagg ccgttttgca
      961 tggcttatat ctctgcagac cagcataaaa tcatgcagca gttccaggag ctttcagccg
     1021 aattttccag agcttctgag tgcttgaaga ctggcaacag gaaggcattt gctggggaac
     1081 ttgaaaaaaa gctgaaagac ttggattaca ccaggacagt gctacacaca gaaacggaga
     1141 tccagaagaa agccaacgac aaaggctttt actcatctca ggcaattgag aaagccaatg
     1201 aactggccag tgtggagaag tccatcattg aacatcaaga cctgctgaag cagatccgct
     1261 cataccctca tcggaagttg aaggggcatg atttgtgtcc tggtgagatg gagcacatcc
     1321 aggatcaggc cagccaggca tccactacct ctaaccctga tgagtctgcc gacacagacc
     1381 tttacacctg cagaccagcc tacaccccaa aacttatcaa agcaaagtcc accaagtgtt
     1441 ttgacaagaa gttgaagacc ttggaggagc tctgtgacac tgaatatttc acccagaccc
     1501 tggctcagct cagccacatt gaacacatgt tcagaggaga cctgtgttac ctcctgacca
     1561 gtcagattga tagagcactt ctaaaacaac agcatataac aaactttctc tttgaagact
     1621 ttgtggaggt cgatgacagg atggtggaga aacaagaaag cataccctct aagcccagtc
     1681 aagacaggcc gccttccagt tctctagaag aatgcccaat tcctaaagtg ttaattagtg
     1741 ttggttctta caagtccagt gtggagtctg tgttgatcaa gatggagcag gaactgggag
     1801 atgaggagta caaggaagtg gaagtgactg agttgagcag tttcgacccc caggaaaact
     1861 tggactacct ggatatggat atgaaaggga gtatcagcag tggtgaaagt attgaagttt
     1921 tgggcacgga gaaatccacc tccgtgcttt ctaaatctga cagccaggca agcctcacag
     1981 taccattgag cccccaggtg gtccggagca aagcagtcag ccacaggacc atcagcgagg
     2041 acagtattga agtcctcagt acctgcccct ctgaggccct catccctgat gactttaagg
     2101 ccagctaccc aagtgccatt aatgaagaag aatcatatcc agatggcaat gaaggagcca
     2161 tccgcttcca ggcaagcatc agtcctccag aactgggtga gacagaggaa ggcagcatag
     2221 aaaacacccc atcacaaata gactcctcct gctgtattgg gaaggagagc gatggtcagt
     2281 tggtgctgcc ctccactcca gcccacacac actctgacga ggatggggtg gtgagcagcc
     2341 ccccacagcg ccacaggcag aaggaccagg ggttccgtgt agacttttca gtggaaaatg
     2401 ccaacccttc ttcccgagac aacagttgtg aagggtttcc cgcttatgag ctggacccga
     2461 gccacctgct ggctagccgg gacatcagta agaccagcct ggacaactac tcagacacca
     2521 ccagctacgt gagcagtgta gcgtccacca gctcagacag gatcccctct gcttatcctg
     2581 ctggcctgtc ttccgatagg cataaaaaga gggctggcca gaacgcctta aaattcatcc
     2641 gccagtaccc ctttgcccac ccagccatct actccctgct cagtgggagg acacttgtgg
     2701 tcctggggga agatgaggcc atagtcagga aactcgtgac tgcactggct atctttgtcc
     2761 ccagctatgg ctgctacgct aagcccgtga aacattgggc ctcctcccct ttgcacatta
     2821 tggattttca gaagtggaag cttattggct tgcagagagt ggcctcccct gccggtgccg
     2881 gtaccctcca tgccctgagc cgctacagcc gctacacgag catcctggac cttgacaaca
     2941 aaaccctgcg ctgccccctt tacagaggca ccctggtgcc ccgcctggca gaccaccgca
     3001 cacagatcaa gcggggcagc acctactacc tgcatgtcca gagcatgctc acccagctct
     3061 gctccaaggc cttcctctac accttctgcc accacctgca cctgcctacc cacgacaagg
     3121 agacagagga gctggtagcc agccgccaga tgagcttcct aaagctgacc ctgggtctgg
     3181 tgaatgagga tgttagggtg gtccagtacc tggctgagct gctgaagctg cactacatgc
     3241 aggaatctcc agggaccagc caccccatgc tcaggtttga ctatgtcccc agctttttgt
     3301 ataaaatctg aggtcggtcc cagacactgt gaccaagacc tgtgactcag ggtatgggga
     3361 ggggaggggt tggcatgacc aaacagttgc ctgagctgga ctgtttaagt ttctggtgtc
     3421 tggcagcatg gttcccacgt ggctcctagt agtttttaag ttggacatgg gcagaagtgg
     3481 agcctggctc cctctaaagg cgtctggagg gatggtgaca gctacatttg gctccctggt
     3541 gaagagtggc ccctggatat ggagggggcc ttggttttct gaggactggc agccagggca
     3601 gaagggaagc caccagtccc ttggtggggc agggtgaggg caggaaggcc aaagcctgat
     3661 gggaggagca cactggctgt attcagcatt gccggacact tgagtggcaa aatgaacgag
     3721 ggtgacagcc aggtaactgt tgtggtttgg tggaaaacag gagggcagaa cacccatcgc
     3781 ctccttcaga cccagatgca gccctttatt tgcttcctgg agcccactgt cctaggggag
     3841 gccctggacc agccagtggg ggtgagaaag gctgccaccc tctgggtcgt gggaaacatg
     3901 cgaccttggc ctcagggcca cagagcgtga gcagcaccct gtccgctgtc ctcgccacct
     3961 ttacctggga gagaagtgcc tgtgagcggg ccttgggact cactccccat actctttcac
     4021 caggaacgag cagagtgttc actagtccta gtgtgaagcg ggcacaagtg accaaagcca
     4081 atggtgagga ccaccttggg ggctggagag cacagcaggc agagcagggc tgaggcgcca
     4141 tgaggccatc accaccatgc aagggaggag cagccagcag ctctcctggg tggcccactg
     4201 gctcctgccg gccagagcgc atgagcactg accttcctcg tggggaaggt ctgcagaccc
     4261 gtgtgaacat ggcagaggcc acactccagc tcccacccac ataaaccttt gcaaccactt
     4321 cactacctct cactgggtct cgtgggcttg ggacagcaac ggttctgttt agtcagctgg
     4381 tgcaggtggt tggcccagcc gatgagaact caaatagcac ggatttcctg gatgagctac
     4441 cacgtggcgc tcttcaggtt ctgcctctgg gaggctgtgt gcacctcagt caccacagct
     4501 cacggccgtc cccagttgag tcccccttct gaggacaagt ttgaaactgg gaaggagaca
     4561 ccccctggtt ccgccagcac ctatcccact gatagtgatc ccctggcctc cccgagcctc
     4621 cttaggcaat ggccactggt ttgtagacag agagtttctt gcagccttga aaattttatt
     4681 ccctaagtgg ggacagctct ggtattactg cttctttaat ttgttccaca aaaattatgc
     4741 tgaaatggac ctgaatcagc cctgggtgtg cttctgttgg cgcctgctgc tgaggcccgg
     4801 cctgcccatc ggcctgtagt acgccatcga ggtcatcatg gagccagcca gcttctcttc
     4861 tcatcaagga ggcagagacc cctggtggaa gccaagagtg accgaagagg gcttgcatca
     4921 cgtcactcca gtggttccct ccttgtccaa gtcctcgttt taagggcatg gcccgttggc
     4981 tccagccgtt ggcttctgtt cagggtcctt gctcccatca gaggtgtttg attttgctcc
     5041 tggggctgcg atggagaacc ttttgtggca gatggtgctg cctacatctc attggttctc
     5101 tgtacctgtg cccgttgcgc atgttcacag acaggagagt cgccagcaat accttggcag
     5161 cctttaaatg tgaaacgtct gttacttgcg ctggaggcag aggctgtgca gggagcctgc
     5221 ttctaatcgt gggctccctt atctgttacg gttccctccc ctcctgttct agatgctgat
     5281 tcgttttctc actggctggt taattgctgt ctacttaggc ctccaaggcc ataactgtgc
     5341 aacattttat ctgagaagga aggaaggtca gaaagtgatc actggcacca ccactgtggc
     5401 attcctgtca caccaacaca gaaattcaca gctcatactg ttctcacctg ggaaacttgg
     5461 gaagggctga tgagtttggg gcagatcctg aattgcagcc acacttggcc ttctagaagc
     5521 tgaagttgtg ccctcaagtg gtcctaagta gaagtccact tgaggtattt gctggcacac
     5581 accctcaagg gtggttcaga aggtcctgaa acctaaggca aaaggctgct ggggcatgaa
     5641 cagaacagac cgtctcaggg gcagccatgt aagtggcaag cagggctggt ctccctcaag
     5701 gtggttcttc attaagtaaa gatggcttcg tgactagtgt ttagctccca gatttatatt
     5761 tgggttaaaa actaactttt caatgtggca gtttcaaagg attacttgat gcaaagtatg
     5821 tttcctttca aagggatctt gacgtggtac caggactgag tcatgatttg ggcagaggca
     5881 gttaacactg ggaagccctg cccctcccta tgcccccttc actccgggga actgaaacct
     5941 actctgagtc tcacatctct aaacctcagt gctgagacct atgcagtggg actaatctca
     6001 tcaagaattt tcagttgagg ccgggcgcag tggctcacgc ctgtaatccc aacactttgg
     6061 gaggccgagg tgggcggatc acctgaggtc aggagttgga gaccagcctg gccaacatgg
     6121 tgaaacccca tctctactaa aaatacaaag aaagttagcc aggtctggtg gtgcgtgcct
     6181 gtaatcccac ttactcggga ggctgaggca ggagaatcac ttgaacccag gaggcagagg
     6241 ttacagtgag ccgagatcgc gccactgcac tccaccctgg gcaacaagag cgaaaactgt
     6301 ctcaaaaaaa aaaaattttt catttgaggt attcttccag tagaaggtta gtaagttttt
     6361 aatgaaacca ttaaaaatta cacttcccag aaaatagatg acatcagtgc cccttgctac
     6421 tttctcagtc ctcactattg ctttgagggc ccaggtactg aaactggttg tcttgagttt
     6481 tgtgtcagct ttttctccag tccattatcc ccctcccttg cttctgaagc agtctaggtt
     6541 aaactagcca ggcaggtagt tgtggactgg tgattttcaa aagccccact ttagagatca
     6601 ggccacagct ttttatatcg cacaggacac atcagcctga gctgctgcct catgcctgtt
     6661 tccccaggaa cctcactcct ttggtagaac cttgggattt tagaaattgt ggcttttcca
     6721 taactcattt actccaacag ttgaagttac acacattgct cccaaatttg gaaatagacc
     6781 acagtacctt acctttcatt ccccatctgg cctttacctt ctttgcttca gtggttgaaa
     6841 acagttgcca tattcaaagt atagtagatt tcaacctcac acaaatgaca agtcccattt
     6901 tacaatccta ggaaggccca ccaatttcat ttcacgcgcc agggcggctg cagttggagg
     6961 ccgagggcag ccctctgctc actgaatgtc ttgcatgtgc tgactgctgc ccgcagtgct
     7021 gaacatgccc caccgcccag gcccagcact gcttgttggg tcagcatcta gtgctgctgt
     7081 cacatctttg tctgcacagc cagtaggatt gcctcagcca gggggtttat cagaaggtgt
     7141 gcaaggcctt tgggggaact gagcccctat agtgggcagt ctcctttacc ttcccacctc
     7201 cctgaaaagc acagaagaca gtgccttggt ttgtgttttg aagcaaacaa gtcagctttc
     7261 tggctttgcc ccaaaactgt gatggaacat aataaaactg gagatatggt ttttaacact
     7321 gcaaaaagga aaaagcatca agtttctact tctggctgga aagcaaaacc aatctcagct
     7381 gacaaggctg ggcaaactaa gttttcctga gcccattttc ctttgagccc tgacctagcc
     7441 tggccttacc tcattaaggt ttggttaaag cagtggaaag gaggaggagg caggggtgga
     7501 tgggggtgtg gggaggggat gagcactctg cagccgatta atctgttggt aggggcccag
     7561 cttcttggga gtgcttattc agcccaagag tggaggctgt ttacagcgag ccctggagat
     7621 ggcagcttgt ctccagctgg ggaggggtca ggcccctaaa ttgaagacca ctttggtagc
     7681 agaactgtag ggactggtga gtcaactcac agattctgca gcagctgctc cacccacaat
     7741 aaagcaaacg ccgacaggct agaccccaga ttgcaggggc tgccacctac aaggtgggac
     7801 cacaggctgc ctcaccggga ttgtctgcca ctaaatagct ggagtcacag attgagataa
     7861 atgccacctt caaggttgca gtgaaaagca taatcctatg tgatgaattt atatgtgtta
     7921 ttttttaaaa agctatttta ttactgcatg ttcccgtccc gtcttgtgaa tgtgagtccc
     7981 cgccaccacg tgaggtgcag tcgttgcagc ggctggtgca ggagtgcagc tggcgcgtgt
     8041 gtgatagcat ctcgtaggtg ttgctgcaca agagttaacc agagtcaatg ccaaacacat
     8101 agtatgagaa gtgtactttt taagaaatta atttatttga gttcaaatat ttttgaaata
     8161 taaaaattgg ttgtattttt taaagctata attcttgtag acattctgtg gttaaaaatt
     8221 tgattgtgct tattaaaaat ggtcatctat gttttgcact tcagctacgt gaaaataaaa
     8281 tttctttggg aaggtga
//
