LOCUS       NM_015295               8833 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens structural maintenance of chromosomes flexible hinge
            domain containing 1 (SMCHD1), mRNA.
ACCESSION   NM_015295 XM_113962 XM_938891
VERSION     NM_015295.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8833)
  AUTHORS   Wong,M.M., Hachmer,S., Gardner,E., Runfola,V., Arezza,E.,
            Megeney,L.A., Emerson,C.P. Jr., Gabellini,D. and Dilworth,F.J.
  TITLE     SMCHD1 activates the expression of genes required for the expansion
            of human myoblasts
  JOURNAL   Nucleic Acids Res 52 (16), 9450-9462 (2024)
   PUBMED   38994563
  REMARK    GeneRIF: SMCHD1 activates the expression of genes required for the
            expansion of human myoblasts.
REFERENCE   2  (bases 1 to 8833)
  AUTHORS   Engal,E., Sharma,A., Aviel,U., Taqatqa,N., Juster,S.,
            Jaffe-Herman,S., Bentata,M., Geminder,O., Gershon,A., Lewis,R.,
            Kay,G., Hecht,M., Epsztejn-Litman,S., Gotkine,M., Mouly,V.,
            Eiges,R., Salton,M. and Drier,Y.
  TITLE     DNMT3B splicing dysregulation mediated by SMCHD1 loss contributes
            to DUX4 overexpression and FSHD pathogenesis
  JOURNAL   Sci Adv 10 (22), eadn7732 (2024)
   PUBMED   38809976
  REMARK    GeneRIF: DNMT3B splicing dysregulation mediated by SMCHD1 loss
            contributes to DUX4 overexpression and FSHD pathogenesis.
REFERENCE   3  (bases 1 to 8833)
  AUTHORS   Laberthonniere,C., Delourme,M., Chevalier,R., Dion,C., Ganne,B.,
            Hirst,D., Caron,L., Perrin,P., Adelaide,J., Chaffanet,M., Xue,S.,
            Nguyen,K., Reversade,B., Dejardin,J., Baudot,A., Robin,J.D. and
            Magdinier,F.
  TITLE     In skeletal muscle and neural crest cells, SMCHD1 regulates
            biological pathways relevant for Bosma syndrome and
            facioscapulohumeral dystrophy phenotype
  JOURNAL   Nucleic Acids Res 51 (14), 7269-7287 (2023)
   PUBMED   37334829
  REMARK    GeneRIF: In skeletal muscle and neural crest cells, SMCHD1
            regulates biological pathways relevant for Bosma syndrome and
            facioscapulohumeral dystrophy phenotype.
REFERENCE   4  (bases 1 to 8833)
  AUTHORS   Sikrova,D., Testa,A.M., Willemsen,I., van den Heuvel,A.,
            Tapscott,S.J., Daxinger,L., Balog,J. and van der Maarel,S.M.
  TITLE     SMCHD1 and LRIF1 converge at the FSHD-associated D4Z4 repeat and
            LRIF1 promoter yet display different modes of action
  JOURNAL   Commun Biol 6 (1), 677 (2023)
   PUBMED   37380887
  REMARK    GeneRIF: SMCHD1 and LRIF1 converge at the FSHD-associated D4Z4
            repeat and LRIF1 promoter yet display different modes of action.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8833)
  AUTHORS   Bargiela,M., Kueper,J., Serebrakian,A.T., Browne,M.R., Brogna,S.,
            Peacock,Z.S., Bojovic,B., Shaw,N.D. and Liao,E.C.
  TITLE     Nasal Construction in Congenital Arhinia Due to Novel SMCHD1 Gene
            Variant
  JOURNAL   J Craniofac Surg 34 (3), 849-854 (2023)
   PUBMED   36944600
  REMARK    GeneRIF: Nasal Construction in Congenital Arhinia Due to Novel
            SMCHD1 Gene Variant.
REFERENCE   6  (bases 1 to 8833)
  AUTHORS   Lemmers,R.J., Tawil,R., Petek,L.M., Balog,J., Block,G.J.,
            Santen,G.W., Amell,A.M., van der Vliet,P.J., Almomani,R.,
            Straasheijm,K.R., Krom,Y.D., Klooster,R., Sun,Y., den Dunnen,J.T.,
            Helmer,Q., Donlin-Smith,C.M., Padberg,G.W., van Engelen,B.G., de
            Greef,J.C., Aartsma-Rus,A.M., Frants,R.R., de Visser,M.,
            Desnuelle,C., Sacconi,S., Filippova,G.N., Bakker,B., Bamshad,M.J.,
            Tapscott,S.J., Miller,D.G. and van der Maarel,S.M.
  TITLE     Digenic inheritance of an SMCHD1 mutation and an FSHD-permissive
            D4Z4 allele causes facioscapulohumeral muscular dystrophy type 2
  JOURNAL   Nat Genet 44 (12), 1370-1374 (2012)
   PUBMED   23143600
  REMARK    GeneRIF: The study identified SMCHD1 as an epigenetic modifier of
            the D4Z4 metastable epiallele and as a causal genetic determinant
            of facioscapulohumeral muscular dystrophy type 2 and possibly other
            human diseases subject to epigenetic regulation.
REFERENCE   7  (bases 1 to 8833)
  AUTHORS   Blewitt,M.E., Gendrel,A.V., Pang,Z., Sparrow,D.B., Whitelaw,N.,
            Craig,J.M., Apedaile,A., Hilton,D.J., Dunwoodie,S.L.,
            Brockdorff,N., Kay,G.F. and Whitelaw,E.
  TITLE     SmcHD1, containing a structural-maintenance-of-chromosomes hinge
            domain, has a critical role in X inactivation
  JOURNAL   Nat Genet 40 (5), 663-669 (2008)
   PUBMED   18425126
REFERENCE   8  (bases 1 to 8833)
  AUTHORS   Tsuritani,K., Irie,T., Yamashita,R., Sakakibara,Y., Wakaguri,H.,
            Kanai,A., Mizushima-Sugano,J., Sugano,S., Nakai,K. and Suzuki,Y.
  TITLE     Distinct class of putative 'non-conserved' promoters in humans:
            comparative studies of alternative promoters of human and mouse
            genes
  JOURNAL   Genome Res 17 (7), 1005-1014 (2007)
   PUBMED   17567985
REFERENCE   9  (bases 1 to 8833)
  AUTHORS   Volik,S., Raphael,B.J., Huang,G., Stratton,M.R., Bignel,G.,
            Murnane,J., Brebner,J.H., Bajsarowicz,K., Paris,P.L., Tao,Q.,
            Kowbel,D., Lapuk,A., Shagin,D.A., Shagina,I.A., Gray,J.W.,
            Cheng,J.F., de Jong,P.J., Pevzner,P. and Collins,C.
  TITLE     Decoding the fine-scale structure of a breast cancer genome and
            transcriptome
  JOURNAL   Genome Res 16 (3), 394-404 (2006)
   PUBMED   16461635
REFERENCE   10 (bases 1 to 8833)
  AUTHORS   Preston,M.K., Tawil,R. and Wang,L.H.
  TITLE     Facioscapulohumeral Muscular Dystrophy
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301616
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP005061.3, BC006008.1,
            AI904018.1, AP001011.6, DN913642.1, BU153211.1, BP872870.1,
            DB071574.1, BU509173.1, AW503637.1, DA283721.1, DA675822.1 and
            AB014550.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 30, 2019 this sequence version replaced NM_015295.2.
            
            Summary: This gene encodes a protein which contains a hinge region
            domain found in members of the SMC (structural maintenance of
            chromosomes) protein family. [provided by RefSeq, Dec 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853565.14181.1,
                                           SRR14038191.972781.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000320876.11/ ENSP00000326603.7
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-161               AP005061.3         165992-166152
            162-1174            BC006008.1         1-1013
            1175-1507           AI904018.1         113-445
            1508-1655           AP001011.6         55064-55211
            1656-2009           DN913642.1         1-354
            2010-2277           BU153211.1         350-617
            2278-2439           BP872870.1         1-162
            2440-2810           DB071574.1         147-517
            2811-2906           AP001011.6         79728-79823
            2907-3011           BU509173.1         12-116
            3012-3209           AW503637.1         8-205
            3210-3306           DA283721.1         1-97
            3307-3819           DA675822.1         1-513
            3820-7511           AB014550.1         1-3692
            7512-8833           AP001011.6         160903-162224
FEATURES             Location/Qualifiers
     source          1..8833
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.32"
     gene            1..8833
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /note="structural maintenance of chromosomes flexible
                     hinge domain containing 1"
                     /db_xref="GeneID:23347"
                     /db_xref="HGNC:HGNC:29090"
                     /db_xref="MIM:614982"
     exon            1..536
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             351..6368
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /note="SMC hinge domain-containing protein 1"
                     /codon_start=1
                     /product="structural maintenance of chromosomes flexible
                     hinge domain-containing protein 1"
                     /protein_id="NP_056110.2"
                     /db_xref="CCDS:CCDS45822.1"
                     /db_xref="GeneID:23347"
                     /db_xref="HGNC:HGNC:29090"
                     /db_xref="MIM:614982"
                     /translation="MAAADGGGPGGASVGTEEDGGGVGHRTVYLFDRREKESELGDRP
                     LQVGERSDYAGFRACVCQTLGISPEEKFVITTTSRKEITCDNFDETVKDGVTLYLLQS
                     VNQLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNI
                     GVRRIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGY
                     VRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKE
                     KNKEAIYSGYIRNRKPSDSVHITNDDERFLHHLIIEEKEKDSFTAVVITGVQPEHIQY
                     LKNYFHLWTRQLAHIYHYYIHGPKGNEIRTSKEVEPFNNIDIEISMFEKGKVPKIVNL
                     REIQDDMQTLYVNTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLK
                     DEDDEDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNR
                     ISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDC
                     HEKYDKQIKFTLFKGVITRPDLPSKKQGPWATYAAIEWDGKIYKAGQLVKTIKTLPLF
                     YGSIVRFFLYGDHDGEVYATGGEVQIAMEPQALYDEVRTVPIAKLDRTVAEKAVKKYV
                     EDEMARLPDRLSVTWPEGDELLPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGG
                     SKKLLVELKVILHSSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNE
                     SNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLLDLPFRVGVPFNIPLEFQDEFGHTSQ
                     LVTDIQPVLEASGLSLHYEEITKGPNCVIRGVTAKGPVNSCQGKNYNLKVTLPGLKED
                     SQILKIRLLPGHPRRLKVKPDSEILVIENGTAFPFQVEVLDESDNITAQPKLIVHCKF
                     SGAPNLPVYVVDCSSSGTSILTGSAIQVQNIKKDQTLKARIEIPSCKDVAPVEKTIKL
                     LPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREINITSALA
                     EKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPD
                     EPKHLKCEMKGGKTVQMGQELQGEVVIIITDQYGNQIQAFSPSSLSSLSIAGVGLDSS
                     NLKTTFQENTQSISVRGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLIDW
                     PELKESIPVINGRDLQNPIIVQLCDQWDNPAPVQHVKISLTKASNLKLMPSNQQHKTD
                     EKGRANLGVFSVFAPRGEHTLQVKAIYNKSIIEGPIIKLMILPDPEKPVRLNVKYDKD
                     ASFLAGGLFTDFMISVISEDDSIIKNINPARISMKMWKLSTSGNRPPANAETFSCNKI
                     KDNDKEDGCFYFRDKVIPNKVGTYCIQFGFMMDKTNILNSEQVIVEVLPNQPVKLVPK
                     IKPPTPAVSNVRSVASRTLVRDLHLSITDDYDNHTGIDLVGTIIATIKGSNEEDTDTP
                     LFIGKVRTLEFPFVNGSAEIMSLVLAESSPGRDSTEYFIVFEPRLPLLSRTLEPYILP
                     FMFYNDVKKQQQMAALTKEKDQLSQSIVMYKSLFEASQQLLNEMKCQVEEARLKEAQL
                     RNELKIHNIDIPTTQQVPHIEALLKRKLSEQEELKKKPRRSCTLPNYTKGSGDVLGKI
                     AHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTL
                     PDWKRSLPHFRNGKLYFKPIGDPVFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDA
                     ANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCL
                     ILGEQIDLLQQYRSAVCKLDSVNKDLNSQLEYLRTPDMRKKKQELDEHEKNLKLIEEK
                     LGMTPIRKCNDSLRHSPKVETTDCPVPPKRMRREATRQNRIITKTDV"
     exon            537..612
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            613..774
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            775..857
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            858..988
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1103
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1223
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1390
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1391..1481
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..1692
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1693..1813
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..1997
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1998..2192
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2193..2306
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2307..2413
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2414..2496
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2497..2610
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2611..2688
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2689..2808
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2809..2953
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2954..3050
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3051..3123
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3124..3263
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3264..3398
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3399..3626
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3627..3775
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3776..3864
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3865..3983
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3984..4151
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4152..4277
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4278..4357
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4358..4515
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4516..4631
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4632..4696
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4697..4784
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4785..4916
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            4917..5069
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            5070..5196
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            5197..5316
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            5317..5402
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            5403..5525
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            5526..5716
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            5717..5826
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            5827..5897
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            5898..6069
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            6070..6228
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            6229..6343
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     exon            6344..8833
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7400..7405
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /note="hexamer: AATAAA"
     regulatory      7404..7409
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /note="hexamer: AATAAG"
     polyA_site      7424
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /note="major polyA site"
     polyA_site      7429
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
     regulatory      8801..8806
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
                     /note="hexamer: AATAAA"
     polyA_site      8833
                     /gene="SMCHD1"
                     /gene_synonym="BAMS; FSHD2"
ORIGIN      
        1 acagtcccgg cggcggcgct ggtcgcgggt cgcaccgtga ggctcgcgtg ggcggcgata
       61 ggcgctgggc ccgggcccgg tgaggagcgc gccgcgcgtc cccttctcct caggagtggc
      121 gggccgcgga agtgacgtgg tgcacgggca ggagcgcgtt tgaatcggtt cccgggtgat
      181 cctcgcgcct gccgctgctc ggccgccgcc gctgacgagg agctgcagcg cgccgggccg
      241 aggcctcgag ccgccccggg agctggagct gaaggcgccg cgcggagcgc gcacctcagc
      301 cctgagcccg gcggcggcag gcgtcgctgt cttttctcct tttccccaat atggcagcgg
      361 cggacggcgg cgggcctggt ggggcctctg tggggactga ggaggatggc ggaggcgtcg
      421 gccacaggac ggtgtacttg tttgatcggc gcgaaaagga gtccgagctc ggggaccggc
      481 ctctgcaggt cggggagcgc tcggactacg cgggatttcg cgcgtgtgtg tgtcagacac
      541 ttggcatttc acctgaagaa aaatttgtta ttacaacaac aagtaggaaa gaaattacct
      601 gtgataattt tgatgaaact gttaaagatg gagtcacctt atacctgcta cagtcggtca
      661 atcagttact actgacagct acgaaagaac gaattgactt cttacctcac tatgacacac
      721 tggttaaaag tggcatgtat gaatattatg ccagtgaagg acaaaatcct ttgccatttg
      781 ctcttgcgga attaattgac aattcattgt ctgctacttc tcgtaacatt ggggttagaa
      841 gaatacagat caaattgctt tttgatgaaa cacaaggaaa acctgctgtt gcagtgatag
      901 ataatggaag aggaatgacc tctaaacagc ttaacaactg ggccgtgtat aggttgtcaa
      961 aattcacaag gcaaggtgac tttgaaagtg atcattcagg atatgttcgt ccagtaccag
     1021 tgccacgcag tttaaatagt gatatttcct attttggtgt tgggggcaag caagctgtct
     1081 tctttgttgg acaatcagcc agaatgataa gcaaacctgc agattcccaa gatgttcacg
     1141 agcttgtgct ttctaaagaa gattttgaga agaaggagaa aaataaagag gcaatatata
     1201 gtggatatat tagaaacaga aagccctctg attctgttca cattacaaat gatgatgaaa
     1261 gatttctaca tcatcttatc atagaggaga aggaaaaaga tagctttact gctgtggtta
     1321 tcacaggggt acaaccagaa cacatacagt acttgaaaaa ttatttccac ctttggacac
     1381 gacagttagc gcatatttat cactactata ttcatggccc aaaaggaaat gaaatacgaa
     1441 catcaaaaga agttgaacct ttcaacaata ttgatattga aatttctatg tttgaaaaag
     1501 ggaaggtacc taagattgtc aacctaaggg aaatacaaga cgacatgcag acgttgtatg
     1561 taaacacagc agctgatagt tttgaattca aagctcatgt tgaaggagat ggtgtagtgg
     1621 aagggattat ccgttatcat ccattcttat atgatagaga aacttaccct gatgatccat
     1681 gctttccatc aaaattaaaa gatgaagatg atgaagatga ttgtttcata cttgagaaag
     1741 cagctagagg gaaaaggcct atttttgaat gtttttggaa tggacgatta ataccatata
     1801 catcagttga agactttgac tggtgtactc ctcctaagaa gagagggctt gcaccaattg
     1861 aatgctacaa taggatatct ggtgcattat tcactaatga caaattccag gtcagcacaa
     1921 ataaattgac gtttatggat cttgagctaa aattgaaaga taagaacacc ctttttacaa
     1981 ggattttaaa tggacaggaa cagcgaatga aaattgacag agaatttgct ttgtggctga
     2041 aggactgtca tgagaagtat gataaacaaa taaaatttac actttttaag ggagtaatta
     2101 cacgtcctga tcttccttct aaaaagcaag gtccctgggc aacatatgca gcaatagaat
     2161 gggatggaaa gatatacaaa gcaggacagc tggtcaagac aatcaagaca cttcccctct
     2221 tttatggaag catagtaaga ttttttcttt atggcgatca tgatggagaa gtatatgcta
     2281 caggaggaga ggttcaaatt gcaatggaac ctcaggcact atatgatgaa gtaagaactg
     2341 tgccaattgc aaagctggat aggacagttg ctgagaaagc tgttaaaaaa tatgtagaag
     2401 atgaaatggc aaggctccct gatagattgt cagtaacttg gcctgaagga gatgaattat
     2461 tgcctaatga ggttaggcct gctggaaccc ctattggtgc gttaagaatt gaaatactga
     2521 ataaaaaagg ggaagcaatg caaaagcttc caggaacaag ccatggaggg tcaaagaaac
     2581 tcctggttga gctcaaagtt attttacatt cttcaagtgg aaataaagag attatttcgc
     2641 atattagtca acatggagga aaatggcctt actggtttaa aaaaatggaa aatattcaga
     2701 agttggggaa ttataccttg aaattacaag ttgtgttgaa tgaaagtaat gcagacactt
     2761 atgcaggaag accactacca tctaaagcaa ttaagttttc tgttaaagag ggtaagccag
     2821 agaaattttc atttggtctt ctggatcttc cttttcgtgt tggagttcca tttaatatcc
     2881 ctctggagtt tcaggatgaa tttggtcata ccagtcaact agtaactgat attcagccag
     2941 ttcttgaagc aagtggttta tctttacatt atgaagaaat aaccaaagga ccaaattgtg
     3001 taattcgagg tgttacagcc aagggccctg taaactcttg tcaaggcaag aattataatc
     3061 tgaaggttac tctgcctggc ttaaaagaag actcacagat tttgaaaatt agattactac
     3121 ctggtcaccc tcgtcgactg aaagtgaaac ctgattctga aattttagtt atagaaaatg
     3181 gaacagcttt cccatttcag gtggaagttt tagatgaatc agacaacata acagcacaac
     3241 caaaattgat tgttcattgt aagttttcag gtgctccaaa ccttccagtc tatgttgtag
     3301 attgcagtag ttctggaacc agtattttaa caggatctgc aattcaagtt cagaatatta
     3361 aaaaagacca gacgcttaaa gcaagaattg aaatacctag ttgtaaagat gtggcacctg
     3421 tggagaagac tattaagttg cttcccagta gccatgttgc aagactacaa atattcagtg
     3481 tagaaggaca aaaggcaatt cagatcaaac atcaggatga ggttaattgg atagcgggtg
     3541 atattatgca taatcttatt tttcaaatgt atgatgaagg agaaagagaa atcaatataa
     3601 catcagcttt agcagaaaaa attaaagtta attggactcc tgagattaac aaagaacact
     3661 tgctacaggg tctgcttcct gatgtgcaag taccaacatc tgtaaaagat atgcgctatt
     3721 gccaggtttc attccaagat gatcatgtgt ctttggaaag tgcgtttaca gtaagaccac
     3781 ttcctgatga acctaaacat ttaaaatgtg aaatgaaagg aggaaaaaca gtacagatgg
     3841 gccaagagct tcaaggagaa gtagttataa taattacaga tcagtacgga aatcagattc
     3901 aagcattttc accaagttct ttatcttctt tgtcaattgc tggggttgga cttgatagct
     3961 caaatttgaa aacaaccttt caggaaaaca cacagagtat aagtgtaaga ggcatcaaat
     4021 ttattccagg tcctcctgga aataaggatc tttgttttac ttggcgtgag ttttctgact
     4081 ttattcgagt gcaactaatt tctggacctc ctgctaaact tctccttata gactggccag
     4141 aactaaagga gtccattcca gtgattaatg gaagagattt acagaaccct attattgttc
     4201 aactttgtga tcagtgggat aatccagcac cggtacaaca tgttaaaata agtcttacaa
     4261 aagctagcaa tttaaagctc atgccttcaa accaacagca taaaacagat gagaaaggca
     4321 gggctaattt gggagtattc agtgtttttg cccctagggg agagcatact cttcaggtta
     4381 aagccatcta taacaaaagt atcatagaag gacctataat taagttaatg attcttccag
     4441 acccagaaaa acccgttcgt ctcaatgtta aatatgacaa agatgcatcc ttcttagcag
     4501 ggggtctttt cactgatttt atgattagtg ttatttctga agatgacagt atcattaaaa
     4561 acattaatcc agcacgtatt tccatgaaaa tgtggaagct gtctaccagt gggaaccgac
     4621 ccccagcaaa tgcagaaaca tttagttgta ataaaataaa agataatgac aaagaagatg
     4681 gctgcttcta tttcagggat aaagtaattc ctaataaagt ggggacatat tgtatccagt
     4741 ttggttttat gatggataaa acaaatattc tcaacagtga acaggttata gttgaagtcc
     4801 tgcctaatca acctgtgaag ttagtaccta aaattaaacc acctacacca gctgtttcaa
     4861 atgttcgctc agttgccagt aggaccttgg tcagagatct acatcttagt atcacggatg
     4921 actacgacaa ccatactgga attgatttgg ttggcactat aatagccacc attaaaggct
     4981 ctaatgagga agatactgat accccacttt ttattgggaa agttagaaca cttgaattcc
     5041 ccttcgtgaa tggttcggct gaaatcatga gtctggtgct ggcagaaagt agtcctggaa
     5101 gggatagtac tgaatatttt attgtatttg agccccggct accactttta tcaagaacct
     5161 tagaaccata tatcctaccg ttcatgtttt acaatgatgt taagaagcag caacaaatgg
     5221 cagcacttac aaaagaaaag gaccaattat ctcagtctat tgttatgtat aaaagtttat
     5281 ttgaagccag ccaacagctt cttaatgaaa tgaaatgtca agttgaagaa gcaagattaa
     5341 aagaggccca attgcgaaat gaactaaaaa tacataatat tgacattcct acaacacaac
     5401 aggtgccaca cattgaagca cttctgaaaa gaaagctatc agaacaagaa gaactgaaga
     5461 aaaaacctag aagatcgtgt actcttccaa actatactaa aggcagtgga gatgttttgg
     5521 gaaagattgc acatctagca caaattgaag atgatagagc tgcgatggtt atttcttggc
     5581 atctggcaag tgacatggac tgtgtagtca ccctaaccac tgacgctgca cgtcgtatct
     5641 atgatgaaac ccaaggtcgt cagcaggtgt tgccccttga ttctatttac aagaagactc
     5701 ttccagattg gaaaagatct ctacctcatt tccgaaatgg aaaattgtat tttaaaccca
     5761 ttggagatcc agtctttgct cgagacttgt taacatttcc agataatgta gaacattgtg
     5821 aaacagtatt tggtatgctg ttaggagaca ccattatttt ggataatctg gatgcggcca
     5881 atcattatag aaaagaggtt gttaaaatta cacactgtcc tacactgctg accagagatg
     5941 gagatcgaat tcgaagtaat ggaaagtttg ggggccttca gaataaagct cctccaatgg
     6001 ataaacttcg gggaatggta tttggagctc cagttccaaa acagtgtctg atcttagggg
     6061 aacaaataga tcttcttcag cagtatcgtt ctgctgtgtg caaactagac agtgtgaata
     6121 aggatcttaa cagtcaatta gagtaccttc gcactccgga tatgaggaag aaaaagcaag
     6181 aacttgatga acatgagaaa aatctcaaac taatagagga aaaactaggt atgactccca
     6241 tacgtaagtg taatgactca ttgcgtcatt caccaaaggt tgagacgaca gattgtccag
     6301 ttcctcctaa aagaatgaga cgagaagcta caagacaaaa taggattata accaaaacag
     6361 atgtatgaga ggtgacagag agaagaggcc attggtctca gtaagaatgc cctgctttct
     6421 gcatctctgt ttcagaagac caagagggtg acttaccaga ctgagtattt ctggggacaa
     6481 tacaagtacc tgggcatgaa tttccatttc gattcagatg ggactggaaa caaccattca
     6541 attttatgaa tcttactgga cattatggat ttactggaat tattccagac attatgccct
     6601 ttggttgtca ctaccttgca aatgtgtaag aggaaaatgt gctaatgtgg cagtgactgt
     6661 aaaactggca catggcattt attaatcctg aagaaaagta catgtactat ttttcagtat
     6721 aaatataatg aacatgtcag aactatttct tgaaaacctt tttattactt ttgcgtgaat
     6781 ttatttaaca aagatgtttt gtcttttgtg taagggaggt tctagaggct agatgtttaa
     6841 ttgtaaatat gtgaggaaac tcaatgcaga attcaggata aaaattttaa aagcacaggt
     6901 atttgggaat tgaaatgtta agatacccag aacaacatta aatcaatgag tgaacttgtg
     6961 acagtggtag catttcaaat ttcaaaagac ttatcctgtg tgtgtgtgtg tgtgtatata
     7021 tatatatata tataaatata tatatataaa atattcagca gcaccaagtt ttataactat
     7081 tgtttgtttg actttattaa tactagaata tgtagtctca gccttaattt tacatttaca
     7141 ttattttgta attttttatt actattttta aggggttaaa gagaacatac attctcacat
     7201 tagtgtactt tctggtagaa agttgctgca aaaacatttg aaatgtatat taacctaatg
     7261 tatgtcatat atatgtcttt gtgtaagttc aagactattg atctgtgaag ttattttgta
     7321 aggacataca tttggtaagt aagtttgtgt cccaggaaat gtatgtgttt ttaaaccctt
     7381 tctaaatatg caggccatta ataaataaga ttgtttcttc cctactgaat agataagtgt
     7441 ttttcttttt taaaattgga agcttcataa aagttatctt gttaaaaaac gatgatgatg
     7501 ttaacctatc tatctttata attggaaatt atttaaactg tttgttgtta cagaagaaac
     7561 aaaatggtaa ttacagaatt agctgtgggg aagattggct cccatggtac tacaggttga
     7621 gtatccctta ttcaaaatgg ttgggacctg aagtgttttg gattttggtt ttttgtgggg
     7681 tttttaaatt attattttgg aatatttgca ttatatttac caatttagca tccctaatcc
     7741 aaaaatcttt cattagaaat tggaaatgct tcaatgagca tttcccttat gtgtcatatc
     7801 tgcgctcaaa tgttttgaat tttggaacat ttcttgtttc tgacttttag attagggata
     7861 ctcaccccgt accaatattt ggagtcctta gacattagat tatatgaaaa tgattgattg
     7921 ataggtaaga aaggttaaag agaaatgact ttttaggaac tagacttgaa cgtataatta
     7981 atatgtggaa ctagtttgct tttataattc attgtatcaa gaaggagtca tgttttaaat
     8041 ccaaggcatt gagactatct agaagcaaaa aagttggttt taaaaattct ttttattaga
     8101 agtgtgttta aaaacatgtc tttttttccc ttgagcgaca gtggcctcac tacattgccc
     8161 aggctagtct tgaactcctg ggttcaagca gtcttcctgc cttggcctcc taagtagcag
     8221 ggattacagg catgcaccca catgccaatt tttattgctt aaaatggcga gttctatgga
     8281 tattatctag ccccaagccc atatctgtac acttttgttc attttataat accagaaaga
     8341 atgttctatc agtaaaagaa ggtttttaag tatgaatctc catttttgtg gagtttttct
     8401 accctgtaaa aattaacttt gtggttccca gtaattacag taaggagttt ttctatattt
     8461 tcacagttgg atttatctaa ggatatttct cagcatatta aggaatcctt ttctacattt
     8521 gagcaaatac tgaggttcat gttgtaccaa ataataataa attgcttttg tgtttaatat
     8581 gtaacacgta agaacaattg aaattttctt ctaagattta atactagtct tttagtataa
     8641 aggaatgtaa ctggagaaaa aaattaatgc tacagccatt tatcctgtgt tatgtgttat
     8701 ataatctgaa atttgtcaca atgttacaat caggaagttt tttttctggt ttcttcttcc
     8761 aaaggaaaat aatagcctac ttgtattttg aagtaactga aataaaactc tctccaagcc
     8821 tttttgcaca tta
//
