LOCUS       NM_032444               7315 bp    mRNA    linear   PRI 17-NOV-2024
DEFINITION  Homo sapiens SLX4 structure-specific endonuclease subunit (SLX4),
            mRNA.
ACCESSION   NM_032444 XM_375298
VERSION     NM_032444.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Kim,S., Park,S.H., Kang,N., Ra,J.S., Myung,K. and Lee,K.Y.
  TITLE     Polyubiquitinated PCNA triggers SLX4-mediated break-induced
            replication in alternative lengthening of telomeres (ALT) cancer
            cells
  JOURNAL   Nucleic Acids Res 52 (19), 11785-11805 (2024)
   PUBMED   39291733
  REMARK    GeneRIF: Polyubiquitinated PCNA triggers SLX4-mediated
            break-induced replication in alternative lengthening of telomeres
            (ALT) cancer cells.
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Alghoul,E., Paloni,M., Takedachi,A., Urbach,S., Barducci,A.,
            Gaillard,P.H., Basbous,J. and Constantinou,A.
  TITLE     Compartmentalization of the SUMO/RNF4 pathway by SLX4 drives DNA
            repair
  JOURNAL   Mol Cell 83 (10), 1640-1658 (2023)
   PUBMED   37059091
  REMARK    GeneRIF: Compartmentalization of the SUMO/RNF4 pathway by SLX4
            drives DNA repair.
REFERENCE   3  (bases 1 to 7315)
  AUTHORS   Katsuki,Y., Abe,M., Park,S.Y., Wu,W., Yabe,H., Yabe,M., van
            Attikum,H., Nakada,S., Ohta,T., Seidman,M.M., Kim,Y. and Takata,M.
  TITLE     RNF168 E3 ligase participates in ubiquitin signaling and
            recruitment of SLX4 during DNA crosslink repair
  JOURNAL   Cell Rep 37 (4), 109879 (2021)
   PUBMED   34706224
  REMARK    GeneRIF: RNF168 E3 ligase participates in ubiquitin signaling and
            recruitment of SLX4 during DNA crosslink repair.
REFERENCE   4  (bases 1 to 7315)
  AUTHORS   Wu,X. and Wang,B.
  TITLE     Abraxas suppresses DNA end resection and limits break-induced
            replication by controlling SLX4/MUS81 chromatin loading in response
            to TOP1 inhibitor-induced DNA damage
  JOURNAL   Nat Commun 12 (1), 4373 (2021)
   PUBMED   34272385
  REMARK    GeneRIF: Abraxas suppresses DNA end resection and limits
            break-induced replication by controlling SLX4/MUS81 chromatin
            loading in response to TOP1 inhibitor-induced DNA damage.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7315)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 7315)
  AUTHORS   Fekairi,S., Scaglione,S., Chahwan,C., Taylor,E.R., Tissier,A.,
            Coulon,S., Dong,M.Q., Ruse,C., Yates,J.R. 3rd, Russell,P.,
            Fuchs,R.P., McGowan,C.H. and Gaillard,P.H.L.
  TITLE     Human SLX4 is a Holliday junction resolvase subunit that binds
            multiple DNA repair/recombination endonucleases
  JOURNAL   Cell 138 (1), 78-89 (2009)
   PUBMED   19596236
  REMARK    GeneRIF: Study reports the identification of Slx4 orthologs in
            metazoa, including fly MUS312, essential for meiotic recombination,
            and human BTBD12, an ATM/ATR checkpoint kinase.
            GeneRIF: show that SLX4 binds the XPF(ERCC4) and MUS81 subunits of
            the XPF-ERCC1 and MUS81-EME1 endonucleases and is required for DNA
            interstrand crosslink repair.
REFERENCE   7  (bases 1 to 7315)
  AUTHORS   Svendsen,J.M., Smogorzewska,A., Sowa,M.E., O'Connell,B.C.,
            Gygi,S.P., Elledge,S.J. and Harper,J.W.
  TITLE     Mammalian BTBD12/SLX4 assembles a Holliday junction resolvase and
            is required for DNA repair
  JOURNAL   Cell 138 (1), 63-77 (2009)
   PUBMED   19596235
  REMARK    GeneRIF: BTBD12/SLX4 was identified as the human ortholog of yeast
            DNA repair factor Slx4p and Drosophila MUS312; SLX4 assembles a
            modular toolkit for repair of specific types of DNA lesions and is
            critical for cellular responses to replication fork failure.
REFERENCE   8  (bases 1 to 7315)
  AUTHORS   Andersen,S.L., Bergstralh,D.T., Kohl,K.P., LaRocque,J.R.,
            Moore,C.B. and Sekelsky,J.
  TITLE     Drosophila MUS312 and the vertebrate ortholog BTBD12 interact with
            DNA structure-specific endonucleases in DNA repair and
            recombination
  JOURNAL   Mol Cell 35 (1), 128-135 (2009)
   PUBMED   19595722
  REMARK    GeneRIF: Genetic and biochemical evidence suggest that MUS312 and
            BTBD12 direct Holliday junction resolution by at least two distinct
            endonucleases in different recombination and repair contexts.
REFERENCE   9  (bases 1 to 7315)
  AUTHORS   Munoz,I.M., Hain,K., Declais,A.C., Gardiner,M., Toh,G.W.,
            Sanchez-Pulido,L., Heuckmann,J.M., Toth,R., Macartney,T.,
            Eppink,B., Kanaar,R., Ponting,C.P., Lilley,D.M. and Rouse,J.
  TITLE     Coordination of structure-specific nucleases by human SLX4/BTBD12
            is required for DNA repair
  JOURNAL   Mol Cell 35 (1), 116-127 (2009)
   PUBMED   19595721
REFERENCE   10 (bases 1 to 7315)
  AUTHORS   Mehta,P.A. and Ebens,C.
  TITLE     Fanconi Anemia
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301575
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY246029.1, BM541728.1,
            BE887716.1, BC029952.1, AI380342.1, DA242337.1, HY050233.1,
            AA279053.1, AK095411.1, AC006111.4 and AL442083.1.
            
            On Nov 23, 2018 this sequence version replaced NM_032444.3.
            
            Summary: This gene encodes a protein that functions as an assembly
            component of multiple structure-specific endonucleases. These
            endonuclease complexes are required for repair of specific types of
            DNA lesions and critical for cellular responses to replication fork
            failure. Mutations in this gene were found in patients with Fanconi
            anemia. [provided by RefSeq, Sep 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000294008.4/ ENSP00000294008.3
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                HY246029.1         40-61
            23-74               BM541728.1         1-52
            75-578              BE887716.1         1-504
            579-2656            BC029952.1         1-2078
            2657-3143           AI380342.1         9-495
            3144-3666           DA242337.1         81-603
            3667-3923           HY050233.1         244-500
            3924-4152           AA279053.1         116-344             c
            4153-5148           AK095411.1         1-996
            5149-5149           AC006111.4         46880-46880         c
            5150-7038           AK095411.1         998-2886
            7039-7315           AL442083.1         1557-1833
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..7315
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /note="SLX4 structure-specific endonuclease subunit"
                     /db_xref="GeneID:84464"
                     /db_xref="HGNC:HGNC:23845"
                     /db_xref="MIM:613278"
     exon            1..47
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /inference="alignment:Splign:2.1.0"
     exon            48..1184
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /inference="alignment:Splign:2.1.0"
     CDS             650..6154
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /note="BTB/POZ domain-containing protein 12"
                     /codon_start=1
                     /product="structure-specific endonuclease subunit SLX4"
                     /protein_id="NP_115820.2"
                     /db_xref="CCDS:CCDS10506.2"
                     /db_xref="GeneID:84464"
                     /db_xref="HGNC:HGNC:23845"
                     /db_xref="MIM:613278"
                     /translation="MKLSVNEAQLGFYLGSLSHLSACPGIDPRSSEDQPESLKTGQMM
                     DESDEDFKELCASFFQRVKKHGIKEVSGERKTQKAASNGTQIRSKLKRTKQTATKTKT
                     LQGPAEKKPPSGSQAPRTKKQRVTKWQASEPAHSVNGEGGVLASAPDPPVLRETAQNT
                     QTGNQQEPSPNLSREKTRENVPNSDSQPPPSCLTTAVPSPSKPRTAQLVLQRMQQFKR
                     ADPERLRHASEECSLEAAREENVPKDPQEEMMAGNVYGLGPPAPESDAAVALTLQQEF
                     ARVGASAHDDSLEEKGLFFCQICQKNLSAMNVTRREQHVNRCLDEAEKTLRPSVPQIP
                     ECPICGKPFLTLKSRTSHLKQCAVKMEVGPQLLLQAVRLQTAQPEGSSSPPMFSFSDH
                     SRGLKRRGPTSKKEPRKRRKVDEAPSEDLLVAMALSRSEMEPGAAVPALRLESAFSER
                     IRPEAENKSRKKKPPVSPPLLLVQDSETTGRQIEDRVALLLSEEVELSSTPPLPASRI
                     LKEGWERAGQCPPPPERKQSFLWEGSALTGAWAMEDFYTARLVPPLVPQRPAQGLMQE
                     PVPPLVPPEHSELSERRSPALHGTPTAGCGSRGPSPSASQREHQALQDLVDLAREGLS
                     ASPWPGSGGLAGSEGTAGLDVVPGGLPLTGFVVPSQDKHPDRGGRTLLSLGLLVADFG
                     AMVNNPHLSDVQFQTDSGEVLYAHKFVLYARCPLLIQYVNNEGFSAVEDGVLTQRVLL
                     GDVSTEAARTFLHYLYTADTGLPPGLSSELSSLAHRFGVSELVHLCEQVPIATDSEGK
                     PWEEKEAENCESRAENFQELLRSMWADEEEEAETLLKSKDHEEDQENVNEAEMEEIYE
                     FAATQRKLLQEERAAGAGEDADWLEGGSPVSGQLLAGVQVQKQWDKVEEMEPLEPGRD
                     EAATTWEKMGQCALPPPQGQHSGARGAEAPEQEAPEEALGHSSCSSPSRDCQAERKEG
                     SLPHSDDAGDYEQLFSSTQGEISEPSQITSEPEEQSGAVRERGLEVSHRLAPWQASPP
                     HPCRFLLGPPQGGSPRGSHHTSGSSLSTPRSRGGTSQVGSPTLLSPAVPSKQKRDRSI
                     LTLSKEPGHQKGKERRSVLECRNKGVLMFPEKSPSIDLTQSNPDHSSSRSQKSSSKLN
                     EEDEVILLLDSDEELELEQTKMKSISSDPLEEKKALEISPRSCELFSIIDVDADQEPS
                     QSPPRSEAVLQQEDEGALPENRGSLGRRGAPWLFCDRESSPSEASTTDTSWLVPATPL
                     ASRSRDCSSQTQISSLRSGLAVQAVTQHTPRASVGNREGNEVAQKFSVIRPQTPPPQT
                     PSSCLTPVSPGTSDGRRQGHRSPSRPHPGGHPHSSPLAPHPISGDRAHFSRRFLKHSP
                     PGPSFLNQTPAGEVVEVGDSDDEQEVASHQANRSPPLDSDPPIPIDDCCWHMEPLSPI
                     PIDHWNLERTGPLSTSSPSRRMNEAADSRDCRSPGLLDTTPIRGSCTTQRKLQEKSSG
                     AGSLGNSRPSFLNSALWDVWDGEEQRPPETPPPAQMPSAGGAQKPEGLETPKGANRKK
                     NLPPKVPITPMPQYSIMETPVLKKELDRFGVRPLPKRQMVLKLKEIFQYTHQTLDSDS
                     EDESQSSQPLLQAPHCQTLASQTYKPSRAGVHAQQEATTGPGAHRPKGPAKTKGPRHQ
                     RKHHESITPPSRSPTKEAPPGLNDDAQIPASQESVATSVDGSDSSLSSQSSSSCEFGA
                     AFESAGEEEGEGEVSASQAAVQAADTDEALRCYIRSKPALYQKVLLYQPFELRELQAE
                     LRQNGLRVSSRRLLDFLDTHCITFTTAATRREKLQGRRRQPRGKKKVERN"
     exon            1185..1409
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1599
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /inference="alignment:Splign:2.1.0"
     exon            1600..1812
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /inference="alignment:Splign:2.1.0"
     exon            1813..2015
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /inference="alignment:Splign:2.1.0"
     exon            2016..2332
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /inference="alignment:Splign:2.1.0"
     exon            2333..2573
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /inference="alignment:Splign:2.1.0"
     exon            2574..2662
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /inference="alignment:Splign:2.1.0"
     exon            2663..2809
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /inference="alignment:Splign:2.1.0"
     exon            2810..2976
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /inference="alignment:Splign:2.1.0"
     exon            2977..5285
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /inference="alignment:Splign:2.1.0"
     exon            5286..5388
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /inference="alignment:Splign:2.1.0"
     exon            5389..5802
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /inference="alignment:Splign:2.1.0"
     exon            5803..7315
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7290..7295
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /note="hexamer: AATAAA"
     polyA_site      7315
                     /gene="SLX4"
                     /gene_synonym="BTBD12; FANCP; MUS312"
                     /note="major polyA site"
ORIGIN      
        1 gcaggcgcgc gagtctccgt taagaaggtg ccgcggcggc gccggagatg tgtaattaag
       61 tgaaccatat atgtttcatc atcatggaga tcttggagaa ttatctgagc accaggttca
      121 tatgtattcg atctcagagg catctattgg acaacaaaac actctttcag ttgtgaactt
      181 tatttattta ttattattat tttttgagac agagttttgc tcttgttgcc caggttagag
      241 tgcagtggca cgatctcggc tcactgcaat ctccgcctcc caggttcaag cgattctctt
      301 gcctctgcct cccgagtagc tgggattaca ggcatctgct accacgcctg gccaattttt
      361 tgtattttca gttgaaacga ggtttcacca tattggccag gctggtctcg aacttctgac
      421 ctcaggtgat ccaccccccg cctcgtcctc caaaagagct gggattacaa gtgtgagcca
      481 ccgcgcccgg cccagttgtg gactttaaca gagggaagct ttaaacatgt ttaaccacag
      541 gcccaatttg aacaaagata cttcaatcat tatagagagg aaaacagtac tttttgttca
      601 attgtgcaaa ctctccaagt atctaatgga gaagtagaga agaaccctaa tgaaactgag
      661 tgtgaatgag gctcagctag gcttctactt gggttcactt tctcatctgt ctgcctgtcc
      721 tgggattgac cctcgctcct ctgaagacca gcctgaaagc cttaaaactg gtcagatgat
      781 ggatgagtct gatgaggact ttaaagaact ctgcgctagc tttttccaaa gggtgaaaaa
      841 acatggaatc aaggaagtgt caggagaaag gaagacacaa aaggctgcct caaacggcac
      901 tcagataaga agcaaattga aaaggaccaa acaaactgct accaagacca aaacccttca
      961 aggccctgca gagaagaaac ctccgtctgg cagccaggcc cctaggacta aaaagcaaag
     1021 ggtaaccaaa tggcaagcaa gtgaaccggc ccactctgtg aatggggagg ggggtgtgct
     1081 tgcctctgct ccagatccac ctgtgctccg ggaaacagca caaaacaccc agacgggtaa
     1141 ccagcaagaa ccatcgccaa acctttccag agagaaaacc agagagaatg tgcccaacag
     1201 cgactcccag cctcctcctt cctgtttgac aacagcagtg ccaagtccct ccaaaccccg
     1261 cacagcacaa ttggtcctac agcgaatgca gcagttcaag agagcagacc ccgagcgttt
     1321 gagacacgct tcagaagagt gctccctcga ggctgcgcgg gaagaaaatg tcccaaagga
     1381 tcctcaagag gagatgatgg cggggaatgt gtatgggctt gggccccctg ccccagagag
     1441 cgacgctgcg gtggccttga ccctgcagca ggagtttgca cgggtaggag catcggcaca
     1501 tgatgatagc ctggaggaaa agggtttgtt cttctgccag atttgtcaaa agaacctctc
     1561 agccatgaac gtgacccgaa gggaacagca tgtgaacagg tgcttggatg aagctgaaaa
     1621 gacactaaga ccttctgtgc ctcagatccc tgagtgcccg atttgtggga aaccgtttct
     1681 taccttaaag agcagaacca gtcacttgaa gcagtgtgct gtgaagatgg aggttggccc
     1741 ccagctcctg cttcaggctg tgcggctgca gacagcacag cctgagggta gcagcagccc
     1801 acccatgttc agcttcagtg atcacagtag aggtctgaaa cggagaggac ccaccagcaa
     1861 gaaggagcca cggaagaggc ggaaggtgga cgaggcaccg tccgaggacc tgctggtggc
     1921 catggctctg tcccggtcgg agatggagcc gggtgcggct gtaccagcgc tcaggctgga
     1981 aagtgccttt tctgagagga taagaccaga agcagagaat aaaagtcgca agaagaaacc
     2041 cccggtatcc cccccattgt tgttagtcca ggactctgaa accacaggcc gacagataga
     2101 ggaccgtgtg gccctgctcc tctctgagga agtggaattg tctagcacgc caccacttcc
     2161 tgccagcagg attttaaagg aagggtggga aagagcgggc cagtgtcctc ctccacctga
     2221 acgcaagcag agctttctgt gggagggcag cgcactgact ggggcctggg ccatggagga
     2281 cttctacacg gccaggctgg tccctcctct cgtgccccag cggcctgccc agggccttat
     2341 gcaggagccc gtgccgcctc tggtgccacc tgagcactca gagctgagcg agcgaaggtc
     2401 acccgctctc cacggcaccc ccactgcagg ctgtggctcc aggggcccgt cgccttcggc
     2461 cagccagagg gagcaccagg ccctgcagga cctcgtggac ctggcgaggg agggactgag
     2521 cgccagcccg tggcccggca gtgggggcct ggctggctcg gaagggactg cagggttgga
     2581 cgtggtgccc ggcggccttc ctctgactgg gtttgtggtg ccatcgcagg acaagcaccc
     2641 ggacaggggc ggccgcacct tgctctccct cgggctgctg gttgctgact ttggcgccat
     2701 ggtcaataac ccacacctga gtgatgtcca gtttcagacg gacagcgggg aggtgcttta
     2761 cgcccacaag ttcgtgcttt atgcccgatg cccgctcctc atccagtatg tgaacaatga
     2821 aggcttctcc gctgtagagg acggggttct gacccagcgt gtcctgctgg gtgacgtgag
     2881 caccgaggcc gcccgcacgt tcctgcacta tctctacact gcggacactg gccttcctcc
     2941 tggccttagc tctgagctga gctccctggc ccacaggttt ggcgtgagtg agctcgttca
     3001 cctgtgcgaa caggtgccta ttgccactga ctcagagggc aaaccatggg aggagaagga
     3061 agcagagaat tgcgaaagca gggccgagaa tttccaggaa ctcttgaggt caatgtgggc
     3121 agatgaagag gaggaagcgg agactttgtt gaaatccaag gaccacgaag aagatcaaga
     3181 aaacgtgaat gaagcagaaa tggaagaaat ttatgaattt gcagctactc agcgaaagct
     3241 tctccaggaa gaaagggcag cgggtgccgg cgaggacgct gactggctgg agggtggcag
     3301 tccggtttct gggcaactcc tagcaggtgt ccaggtgcag aaacagtggg acaaggtgga
     3361 ggagatggag ccgttggagc caggaagaga tgaggccgcc accacctggg agaagatggg
     3421 acagtgcgct ctcccgccac cccagggcca gcactcaggg gcacggggag cagaggcccc
     3481 tgagcaggag gcgccagagg aggcgcttgg ccattccagc tgctccagcc cttccaggga
     3541 ctgccaggca gagagaaaag aaggctctct tccgcactca gatgatgccg gggattacga
     3601 acagctcttc tcatcaactc agggagagat ctcagagccg tcccaaataa caagtgagcc
     3661 cgaggaacaa agtggcgctg tcagggaaag ggggctggag gtttctcatc gcctggctcc
     3721 ctggcaggca tctccaccgc acccgtgccg cttcctattg gggcctcccc agggcgggag
     3781 tccccgcggg tctcatcaca caagtgggtc gtccctgtca acaccccggt cccgtggcgg
     3841 aacttcccag gtgggctccc caaccttgct gtctccagct gtgccatcaa agcagaaaag
     3901 ggacaggagc atcctcacgc tgtctaaaga gccagggcac cagaaaggca aagagcgtcg
     3961 gtccgtgctg gagtgcagaa ataagggggt cctgatgttc ccagaaaaat ctccgtctat
     4021 tgacctaacc cagtcaaatc ctgaccattc gagctccaga tctcagaaat cttcatccaa
     4081 actgaacgaa gaagatgagg tcatcctctt actggactcg gatgaggagc tggagctaga
     4141 acaaaccaaa atgaagtcca tttctagtga tcctctggaa gaaaagaaag ctctagaaat
     4201 tagccctagg tcctgtgagc tgttttccat cattgatgtt gatgcagatc aggaaccttc
     4261 ccagagccca ccaagaagcg aagctgtgct gcagcaggag gatgaggggg cgctgccgga
     4321 gaatcggggc tctttgggca ggagaggggc tccctggctg ttctgtgacc gtgagagcag
     4381 ccccagcgag gccagcacca cagacacctc gtggctggtg cccgccaccc cgctggccag
     4441 cagaagccgt gactgttctt cccagaccca aatcagcagc ctcaggagcg ggctggccgt
     4501 gcaggcggtg actcagcaca cgcccagggc ctcagtagga aacagggaag ggaacgaagt
     4561 cgcacagaag ttttctgtca tcaggcccca gacaccaccg ccccagacac cgtcctcatg
     4621 cctcactccc gtctctccag gaacttctga cggcagaagg caaggccaca gaagcccttc
     4681 ccgtccccac cccgggggcc acccgcactc ctctccgctg gctccacatc ccatctcagg
     4741 ggaccgcgcc cacttcagca ggcggttcct gaaacactcg ccgcctgggc caagcttcct
     4801 gaaccagacc ccagcgggtg aagtggtgga agtcggagac agtgacgatg agcaggaggt
     4861 ggcctcccat caggccaaca gaagcccccc actggacagt gaccccccaa ttccaattga
     4921 cgactgctgc tggcacatgg agcccctctc gccaattccc attgaccact ggaacctgga
     4981 gcggaccggc cccctgagca ccagcagccc cagccgcagg atgaacgagg ccgccgacag
     5041 ccgtgactgt cgctccccgg gactcctgga caccaccccc atccgaggaa gctgcactac
     5101 ccagaggaaa ttgcaagaga agtcctcggg cgcgggctcc ctggggaata gcaggccgag
     5161 ctttctgaat tcggctctgt gggacgtttg ggacggggaa gagcagaggc ctccagagac
     5221 ccctcctccg gcccagatgc caagcgctgg tggagctcag aagcccgaag ggttagagac
     5281 acccaaaggt gctaatcgga agaagaactt gccccccaaa gtgcccataa cgccgatgcc
     5341 acagtattcc attatggaga cgccggtgct gaagaaggaa ctggataggt ttggagtccg
     5401 ccctctgcct aaacgccaga tggttctgaa gctgaaggag atattccagt acactcacca
     5461 gaccctggac tcagactccg aggacgagag ccagtcctca cagccgctgt tgcaggcgcc
     5521 tcactgccag accctcgcct cccagaccta caagccttca agggcagggg tccatgccca
     5581 gcaggaggcc accacaggac ctggggccca taggcccaag ggacctgcta agaccaaggg
     5641 cccccgacat caaaggaagc atcatgaaag catcacaccc ccaagcaggt cgcccaccaa
     5701 ggaggcacct ccaggcctca atgatgacgc ccagatccca gcctctcaag aatccgtggc
     5761 cacctctgtg gatggcagtg acagctcctt gagctcacag agttcttcct cctgtgagtt
     5821 tggagcggca tttgagtctg caggtgaaga ggagggcgag ggggaggtca gtgcctcgca
     5881 ggcagccgtg caggcggcgg acacagacga ggcgctgagg tgctacatcc gctccaagcc
     5941 ggccctgtac cagaaggtgc tgctgtacca gccctttgag ctgcgggagc tgcaggcaga
     6001 gctgaggcag aacggcctcc gtgtgtcctc gcgcaggctg ttggacttcc tggacaccca
     6061 ctgtatcacc ttcaccactg ccgccacccg cagggagaag ctccagggca ggaggcggca
     6121 gcctcggggc aagaagaagg tggagcggaa ctgatggggc catcccgacc ccaccccaac
     6181 ctgccatcag cagcccccac ccccgccatt tgcagggagg acctgggaca cccagcgtgg
     6241 gtcaggcctc cacaggcatt tctgggcctg gggaccacat cagctctgcg ctgtgatgat
     6301 gaccacagcc caatccaggg cttcctcctc tgggctctgc tttctagggt ggcatttgga
     6361 gcatgtcacc cactggattt acagactcca gccccttcct ctgtccgtgc tcacagtgtg
     6421 tctccctttt tggttttctt tttttttttc tttttgagac agtcgtgctg tgtcacctag
     6481 gctggagtgc agtggcacaa tctcggctca ctgcaacctc cgcctcccgg gttcaagcaa
     6541 ttctcctgcc tcagcctccc agatagctgg gactacaggc acacgctgcc acgcccagct
     6601 gattttttat attttagtag aaacggggtt tcaccatgtt gctcaggctg gtcacaaact
     6661 ccagagctca ggcaatccgc ctgctgcggc ctcccaaagt gctgggatca caggcgccag
     6721 ccactgcgcc cggcccagtg tgtctccctt aacccaagag ggccctcagc tgtcccaggg
     6781 ggcagtgggc catcaccagc tggccagggc atggcctatt ctgccacatt tgccaccctc
     6841 tgagcccacc agtcctgggc acagctgccc tacatgtctg tcctgagatg gacgtcaggt
     6901 ccagcctgcc ccggcagccc gggcccgtcc tcctcagcac tcaggccaac cccagccacc
     6961 gccagcctga gaccaggtgt cctgaggctc cctgcactgc cacagcccag atgcagttct
     7021 cctgacccag ccgtgctacc cggacacttg tcattgttac cagcagtctc caaactggac
     7081 agtgcacaag ggcccagaac aactctgatg ccaccacaaa acaaacatgt tcactagcgg
     7141 attccattct ttgggttaaa gctgcctcca gcctcaggag cagtgtggag gaagatgagg
     7201 gccaggaaag aaggaaacct tggtttctcc atccttgtga atgtcctcgt ctgtttcaaa
     7261 tacagtgcag tcagttttat atgatgtgca ataaaccaaa aaggctttat taaaa
//
