LOCUS       NM_004787               8053 bp    mRNA    linear   PRI 11-JUN-2024
DEFINITION  Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 1,
            mRNA.
ACCESSION   NM_004787
VERSION     NM_004787.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8053)
  AUTHORS   Xia,Y., Jiang,T., Li,Y., Gu,C., Lv,J., Lu,C., Xu,P., Fang,L.,
            Chen,Z., Liu,H., Zhang,D., Xu,H., Yang,L., Xu,Z. and Wang,L.
  TITLE     circVAPA-rich small extracellular vesicles derived from gastric
            cancer promote neural invasion by inhibiting SLIT2 expression in
            neuronal cells
  JOURNAL   Cancer Lett 592, 216926 (2024)
   PUBMED   38714291
  REMARK    GeneRIF: circVAPA-rich small extracellular vesicles derived from
            gastric cancer promote neural invasion by inhibiting SLIT2
            expression in neuronal cells.
REFERENCE   2  (bases 1 to 8053)
  AUTHORS   He,C., Gu,L., Li,A., Li,Y., Xiao,R., Liao,J., Mu,J., Gan,Y.,
            Peng,M., Mohan,G., Liu,W., Xu,L. and Guo,S.
  TITLE     Recombinant Slit2 attenuates tracheal fibroblast activation in
            benign central airway obstruction by inhibiting the TGF-beta1/Smad3
            signaling pathway
  JOURNAL   Mol Cell Probes 73, 101947 (2024)
   PUBMED   38122948
  REMARK    GeneRIF: Recombinant Slit2 attenuates tracheal fibroblast
            activation in benign central airway obstruction by inhibiting the
            TGF-beta1/Smad3 signaling pathway.
REFERENCE   3  (bases 1 to 8053)
  AUTHORS   Bhosle,V.K., Sun,C., Patel,S., Ho,T.W.W., Westman,J.,
            Ammendolia,D.A., Langari,F.M., Fine,N., Toepfner,N., Li,Z.,
            Sharma,M., Glogauer,J., Capurro,M.I., Jones,N.L., Maynes,J.T.,
            Lee,W.L., Glogauer,M., Grinstein,S. and Robinson,L.A.
  TITLE     The chemorepellent, SLIT2, bolsters innate immunity against
            Staphylococcus aureus
  JOURNAL   Elife 12, e87392 (2023)
   PUBMED   37773612
  REMARK    GeneRIF: The chemorepellent, SLIT2, bolsters innate immunity
            against Staphylococcus aureus.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8053)
  AUTHORS   Seiradake,E., von Philipsborn,A.C., Henry,M., Fritz,M.,
            Lortat-Jacob,H., Jamin,M., Hemrika,W., Bastmeyer,M., Cusack,S. and
            McCarthy,A.A.
  TITLE     Structure and functional relevance of the Slit2 homodimerization
            domain
  JOURNAL   EMBO Rep 10 (7), 736-741 (2009)
   PUBMED   19498462
  REMARK    GeneRIF: Fourth Slit2 domain heparan sulphate binding contributes
            to a Slit-Robo signalling mechanism more intricate than previously
            thought.
REFERENCE   5  (bases 1 to 8053)
  AUTHORS   Li,H.S., Chen,J.H., Wu,W., Fagaly,T., Zhou,L., Yuan,W., Dupuis,S.,
            Jiang,Z.H., Nash,W., Gick,C., Ornitz,D.M., Wu,J.Y. and Rao,Y.
  TITLE     Vertebrate slit, a secreted ligand for the transmembrane protein
            roundabout, is a repellent for olfactory bulb axons
  JOURNAL   Cell 96 (6), 807-818 (1999)
   PUBMED   10102269
REFERENCE   6  (bases 1 to 8053)
  AUTHORS   Brose,K., Bland,K.S., Wang,K.H., Arnott,D., Henzel,W.,
            Goodman,C.S., Tessier-Lavigne,M. and Kidd,T.
  TITLE     Slit proteins bind Robo receptors and have an evolutionarily
            conserved role in repulsive axon guidance
  JOURNAL   Cell 96 (6), 795-806 (1999)
   PUBMED   10102268
REFERENCE   7  (bases 1 to 8053)
  AUTHORS   Wang,K.H., Brose,K., Arnott,D., Kidd,T., Goodman,C.S., Henzel,W.
            and Tessier-Lavigne,M.
  TITLE     Biochemical purification of a mammalian slit protein as a positive
            regulator of sensory axon elongation and branching
  JOURNAL   Cell 96 (6), 771-784 (1999)
   PUBMED   10102266
REFERENCE   8  (bases 1 to 8053)
  AUTHORS   Georgas,K., Burridge,L., Smith,K., Holmes,G.P., Chenevix-Trench,G.,
            Ioannou,P.A. and Little,M.H.
  TITLE     Assignment of the human slit homologue SLIT2 to human chromosome
            band 4p15.2
  JOURNAL   Cytogenet Cell Genet 86 (3-4), 246-247 (1999)
   PUBMED   10575218
REFERENCE   9  (bases 1 to 8053)
  AUTHORS   Itoh,A., Miyabayashi,T., Ohno,M. and Sakano,S.
  TITLE     Cloning and expressions of three mammalian homologues of Drosophila
            slit suggest possible roles for Slit in the formation and
            maintenance of the nervous system
  JOURNAL   Brain Res Mol Brain Res 62 (2), 175-186 (1998)
   PUBMED   9813312
REFERENCE   10 (bases 1 to 8053)
  AUTHORS   Robertson,N.G., Khetarpal,U., Gutierrez-Espeleta,G.A., Bieber,F.R.
            and Morton,C.C.
  TITLE     Isolation of novel and known genes from a human fetal cochlear cDNA
            library using subtractive hybridization and differential screening
  JOURNAL   Genomics 23 (1), 42-50 (1994)
   PUBMED   7829101
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA721496.1, AC096718.3,
            BC117190.1, AK126459.1, DB207352.1, CV370469.1, BF112143.1,
            AC021118.6, BM474839.1, BI494498.1 and AA489463.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_004787.3.
            
            Summary: This gene encodes a member of the slit family of secreted
            glycoproteins, which are ligands for the Robo family of
            immunoglobulin receptors. Slit proteins play highly conserved roles
            in axon guidance and neuronal migration and may also have functions
            during other cell migration processes including leukocyte
            migration. Members of the slit family are characterized by an
            N-terminal signal peptide, four leucine-rich repeats, nine
            epidermal growth factor repeats, and a C-terminal cysteine knot.
            Proteolytic processing of this protein gives rise to an N-terminal
            fragment that contains the four leucine-rich repeats and five
            epidermal growth factor repeats and a C-terminal fragment that
            contains four epidermal growth factor repeats and the cysteine
            knot. Both full length and cleaved proteins are secreted
            extracellularly and can function in axon repulsion as well as other
            specific processes. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB017168.1, BC117190.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000504154.6/ ENSP00000422591.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-310               DA721496.1         1-310
            311-1832            AC096718.3         99346-100867
            1833-6526           BC117190.1         1-4694
            6527-6658           AK126459.1         2816-2947
            6659-6943           DB207352.1         292-576
            6944-6966           CV370469.1         102-124             c
            6967-7210           BF112143.1         3-246               c
            7211-7271           AC021118.6         150281-150341
            7272-7767           BM474839.1         1-496
            7768-8049           BI494498.1         123-404
            8050-8053           AA489463.1         5-8                 c
FEATURES             Location/Qualifiers
     source          1..8053
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p15.31"
     gene            1..8053
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /note="slit guidance ligand 2"
                     /db_xref="GeneID:9353"
                     /db_xref="HGNC:HGNC:11086"
                     /db_xref="MIM:603746"
     exon            1..2090
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             1912..6501
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; slit homolog 2 protein"
                     /codon_start=1
                     /product="slit homolog 2 protein isoform 1 precursor"
                     /protein_id="NP_004778.1"
                     /db_xref="CCDS:CCDS3426.1"
                     /db_xref="GeneID:9353"
                     /db_xref="HGNC:HGNC:11086"
                     /db_xref="MIM:603746"
                     /translation="MRGVGWQMLSLSLGLVLAILNKVAPQACPAQCSCSGSTVDCHGL
                     ALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDL
                     KELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDY
                     NQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLA
                     WLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAA
                     CTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNN
                     QISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAF
                     QDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPI
                     ETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPE
                     KCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFS
                     NNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGND
                     SFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKK
                     RIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRC
                     SNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFS
                     NMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAI
                     GANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVN
                     ILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTC
                     HLKEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTG
                     ELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNG
                     AHCTDAVNGYTCICPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRINEPICQ
                     CLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKD
                     HIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGNPK
                     IITNLSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDF
                     QKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGN
                     KCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYC
                     ECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGP
                     LRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCVS"
     exon            2091..2162
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2163..2234
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2235..2306
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2307..2378
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2379..2450
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2451..2522
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2523..2686
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2687..2825
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2826..2897
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2898..2969
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2970..3041
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3042..3185
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3186..3349
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3350..3373
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3374..3524
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3525..3599
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3600..3743
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3744..3887
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3888..4054
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4055..4187
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4188..4256
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4257..4328
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4329..4400
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4401..4472
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4473..4636
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4637..4761
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4762..4859
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4860..4999
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5000..5093
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5094..5231
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5232..5472
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5473..5603
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5604..5758
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5759..6047
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            6048..6259
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            6260..8053
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7780..7785
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /note="hexamer: AATAAA"
     polyA_site      7803
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /note="major polyA site"
     regulatory      8029..8034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /note="hexamer: ATTAAA"
     polyA_site      8053
                     /gene="SLIT2"
                     /gene_synonym="SLIL3; Slit-2"
                     /note="major polyA site"
ORIGIN      
        1 acttggtgtt atttatttgg gaagcgcccg gacggcggag cttggcggcg gcggtggtgg
       61 tggctgccgc agactgtggt taaaaaaaag aaggcggcgg cggcggcggc ggcggaggcg
      121 gaggcagctg cgaggcatgg gagcgccgaa gcgcccaggc gcaggccgaa gctgccgcgc
      181 tttctgggca cggcgggagt gctgagcaga aaggggagcg ccgggggccc gcagccggct
      241 ccggaggcgc gggccgggtt tttgtttggc tacgctgagc gccagtcagc cccagcgaac
      301 aactccagtt acgacaacaa cccaccttcc ttccagacaa gcgagacttg ggctgctgcg
      361 tccgtcctat tgtttagaca cttgccaggg gctccggagt cggcagagcc accgagtccc
      421 cgctctgagt cgtcgccctc cctctccccg acctcgctcc ctggagcggg aggccaggaa
      481 agcagcggga gaggggaagg ggctagaagg agaaggacta cccgggactg cggccgccgc
      541 gtcaggtgca gcgccaggag ccgggcggcg tcgccacgcc ggcaggggta ccgccactgt
      601 ggccttgggg gacggaattc aaagcctggg aaaagttgct gcactttgag aaggacgaac
      661 cactagtggg agaccgccgg gggccggccg tggctctgcg ccctccggaa cccggctctt
      721 gtttcttcta cctttgccat caggtgtctg ccgcggagct gcggcttatc tgggagacga
      781 gcggggttga cacgcgcgca cacactactg ccattcagct gccgcctggc tctgcctgga
      841 gtagtggatc ccacccgccc acctgccacc gagccattct ccagtacgcc ccagcaggac
      901 gctgacacct ccaaccttgg cctttgcctt tccactcctt ccggtctgcc tggtttttaa
      961 gtccgccccc agtcagtccc cactcagtct tcgcagcagc tctcatcctc cacttggcct
     1021 cttggagttc ctcgccggag tgctgactag tggatatttc tgcccggctg cggcggcccg
     1081 actgcccttt tgtcttttct gcgtgacctc ggggcaggtc ctggtgcaga gcgtcgccaa
     1141 ggacgccgag cgggaggcgg gattgcccag acatccttca gcgaagtgca tgtgtgtttg
     1201 taaaccatcg ttggctgtcg ggagaccgcg aggaccggtc caggctgcgg cggagtcgag
     1261 ggcgagggag aggccgcgtg agtgagcaga gtccagagcc gtgcgccccc agaactgcgc
     1321 gtccgccccg tgcacccccg cgcgccatgc ccagttgccc cgcgcgctct gctacgggcc
     1381 cgctgggctt ccgcgccttc tagcttccgg agcccacttt gatcggggcc ataataccta
     1441 ttgagatccc ctcttctgtc ttgtaccttc gccactggca tcggatttgc agaagcgtgc
     1501 gtgggatcag aggaccgccc tccccacaac aaccggcccc tgcatcttag cagccgttgg
     1561 aagccccagc tcttttaccg ccaagttcat ccttgggaga cagaagacgc gtgatctcct
     1621 ctccgctgct cttggggtct ccttgcagcc ctggccaggc ggattcatcc tcaggaccta
     1681 aagttgccca aggagctcct gctctgccag aggagggtgg agagggcggt gggaggcgtg
     1741 tgcctgagtg ggctctactg ccttgttcca tattatttgg tgcacatttt ccctggcact
     1801 ctgggttgct agccccgccg ggcactgggc ctcagacact gcgcggttcc ctcggagcag
     1861 caagctaaag aaagccccca gtgccggcga ggaaggaggc ggcggggaaa gatgcgcggc
     1921 gttggctggc agatgctgtc cctgtcgctg gggttagtgc tggcgatcct gaacaaggtg
     1981 gcaccgcagg cgtgcccggc gcagtgctct tgctcgggca gcacagtgga ctgtcacggg
     2041 ctggcgctgc gcagcgtgcc caggaatatc ccccgcaaca ccgagagact ggatttaaat
     2101 ggaaataaca tcacaagaat tacgaagaca gattttgctg gtcttagaca tctaagagtt
     2161 cttcagctta tggagaataa gattagcacc attgaaagag gagcattcca ggatcttaaa
     2221 gaactagaga gactgcgttt aaacagaaat caccttcagc tgtttcctga gttgctgttt
     2281 cttgggactg cgaagctata caggcttgat ctcagtgaaa accaaattca ggcaatccca
     2341 aggaaagctt tccgtggggc agttgacata aaaaatttgc aactggatta caaccagatc
     2401 agctgtattg aagatggggc attcagggct ctccgggacc tggaagtgct cactctcaac
     2461 aataacaaca ttactagact ttctgtggca agtttcaacc atatgcctaa acttaggact
     2521 tttcgactgc attcaaacaa cctgtattgt gactgccacc tggcctggct ctccgactgg
     2581 cttcgccaaa ggcctcgggt tggtctgtac actcagtgta tgggcccctc ccacctgaga
     2641 ggccataatg tagccgaggt tcaaaaacga gaatttgtct gcagtggtca ccagtcattt
     2701 atggctcctt cttgtagtgt tttgcactgc cctgccgcct gtacctgtag caacaatatc
     2761 gtagactgtc gtgggaaagg tctcactgag atccccacaa atcttccaga gaccatcaca
     2821 gaaatacgtt tggaacagaa cacaatcaaa gtcatccctc ctggagcttt ctcaccatat
     2881 aaaaagctta gacgaattga cctgagcaat aatcagatct ctgaacttgc accagatgct
     2941 ttccaaggac tacgctctct gaattcactt gtcctctatg gaaataaaat cacagaactc
     3001 cccaaaagtt tatttgaagg actgttttcc ttacagctcc tattattgaa tgccaacaag
     3061 ataaactgcc ttcgggtaga tgcttttcag gatctccaca acttgaacct tctctcccta
     3121 tatgacaaca agcttcagac catcgccaag gggacctttt cacctcttcg ggccattcaa
     3181 actatgcatt tggcccagaa cccctttatt tgtgactgcc atctcaagtg gctagcggat
     3241 tatctccata ccaacccgat tgagaccagt ggtgcccgtt gcaccagccc ccgccgcctg
     3301 gcaaacaaaa gaattggaca gatcaaaagc aagaaattcc gttgttcagc taaagaacag
     3361 tatttcattc caggtacaga agattatcga tcaaaattaa gtggagactg ctttgcggat
     3421 ctggcttgcc ctgaaaagtg tcgctgtgaa ggaaccacag tagattgctc taatcaaaag
     3481 ctcaacaaaa tcccggagca cattccccag tacactgcag agttgcgtct caataataat
     3541 gaatttaccg tgttggaagc cacaggaatc tttaagaaac ttcctcaatt acgtaaaata
     3601 aactttagca acaataagat cacagatatt gaggagggag catttgaagg agcatctggt
     3661 gtaaatgaaa tacttcttac gagtaatcgt ttggaaaatg tgcagcataa gatgttcaag
     3721 ggattggaaa gcctcaaaac tttgatgttg agaagcaatc gaataacctg tgtggggaat
     3781 gacagtttca taggactcag ttctgtgcgt ttgctttctt tgtatgataa tcaaattact
     3841 acagttgcac caggggcatt tgatactctc cattctttat ctactctaaa cctcttggcc
     3901 aatcctttta actgtaactg ctacctggct tggttgggag agtggctgag aaagaagaga
     3961 attgtcacgg gaaatcctag atgtcaaaaa ccatacttcc tgaaagaaat acccatccag
     4021 gatgtggcca ttcaggactt cacttgtgat gacggaaatg atgacaatag ttgctcccca
     4081 ctttctcgct gtcctactga atgtacttgc ttggatacag tcgtccgatg tagcaacaag
     4141 ggtttgaagg tcttgccgaa aggtattcca agagatgtca cagagttgta tctggatgga
     4201 aaccaattta cactggttcc caaggaactc tccaactaca aacatttaac acttatagac
     4261 ttaagtaaca acagaataag cacgctttct aatcagagct tcagcaacat gacccagctc
     4321 ctcaccttaa ttcttagtta caaccgtctg agatgtattc ctcctcgcac ctttgatgga
     4381 ttaaagtctc ttcgattact ttctctacat ggaaatgaca tttctgttgt gcctgaaggt
     4441 gctttcaatg atctttctgc attatcacat ctagcaattg gagccaaccc tctttactgt
     4501 gattgtaaca tgcagtggtt atccgactgg gtgaagtcgg aatataagga gcctggaatt
     4561 gctcgttgtg ctggtcctgg agaaatggca gataaacttt tactcacaac tccctccaaa
     4621 aaatttacct gtcaaggtcc tgtggatgtc aatattctag ctaagtgtaa cccctgccta
     4681 tcaaatccgt gtaaaaatga tggcacatgt aatagtgatc cagttgactt ttaccgatgc
     4741 acctgtccat atggtttcaa ggggcaggac tgtgatgtcc caattcatgc ctgcatcagt
     4801 aacccatgta aacatggagg aacttgccac ttaaaggaag gagaagaaga tggattctgg
     4861 tgtatttgtg ctgatggatt tgaaggagaa aattgtgaag tcaacgttga tgattgtgaa
     4921 gataatgact gtgaaaataa ttctacatgt gtcgatggca ttaataacta cacatgcctt
     4981 tgcccacctg agtatacagg tgagttgtgt gaggagaagc tggacttctg tgcccaggac
     5041 ctgaacccct gccagcacga ttcaaagtgc atcctaactc caaagggatt caaatgtgac
     5101 tgcacaccag ggtacgtagg tgaacactgc gacatcgatt ttgacgactg ccaagacaac
     5161 aagtgtaaaa acggagccca ctgcacagat gcagtgaacg gctatacgtg catatgcccc
     5221 gaaggttaca gtggcttgtt ctgtgagttt tctccaccca tggtcctccc tcgtaccagc
     5281 ccctgtgata attttgattg tcagaatgga gctcagtgta tcgtcagaat aaatgagcca
     5341 atatgtcagt gtttgcctgg ctatcaggga gaaaagtgtg aaaaattggt tagtgtgaat
     5401 tttataaaca aagagtctta tcttcagatt ccttcagcca aggttcggcc tcagacgaac
     5461 ataacacttc agattgccac agatgaagac agcggaatcc tcctgtataa gggtgacaaa
     5521 gaccatatcg cggtagaact ctatcggggg cgtgttcgtg ccagctatga caccggctct
     5581 catccagctt ctgccattta cagtgtggag acaatcaatg atggaaactt ccacattgtg
     5641 gaactacttg ccttggatca gagtctctct ttgtccgtgg atggtgggaa ccccaaaatc
     5701 atcactaact tgtcaaagca gtccactctg aattttgact ctccactcta tgtaggaggc
     5761 atgccaggga agagtaacgt ggcatctctg cgccaggccc ctgggcagaa cggaaccagc
     5821 ttccacggct gcatccggaa cctttacatc aacagtgagc tgcaggactt ccagaaggtg
     5881 ccgatgcaaa caggcatttt gcctggctgt gagccatgcc acaagaaggt gtgtgcccat
     5941 ggcacatgcc agcccagcag ccaggcaggc ttcacctgcg agtgccagga aggatggatg
     6001 gggcccctct gtgaccaacg gaccaatgac ccttgccttg gaaataaatg cgtacatggc
     6061 acctgcttgc ccatcaatgc gttctcctac agctgtaagt gcttggaggg ccatggaggt
     6121 gtcctctgtg atgaagagga ggatctgttt aacccatgcc aggcgatcaa gtgcaagcat
     6181 gggaagtgca ggctttcagg tctggggcag ccctactgtg aatgcagcag tggatacacg
     6241 ggggacagct gtgatcgaga aatctcttgt cgaggggaaa ggataagaga ttattaccaa
     6301 aagcagcagg gctatgctgc ttgccaaaca accaagaagg tgtcccgatt agagtgcaga
     6361 ggtgggtgtg caggagggca gtgctgtgga ccgctgagga gcaagcggcg gaaatactct
     6421 ttcgaatgca ctgacggctc ctcctttgtg gacgaggttg agaaagtggt gaagtgcggc
     6481 tgtacgaggt gtgtgtccta aacacactcc cggcagctct gtctttggaa aaggttgtat
     6541 acttcttgac cgtgtgggac taatgaatgc ttcatagtgg aaatatttga aatatattgt
     6601 aaaatacaga acagacttat ttttattatg agaataaaga ctttttttct gcatttggaa
     6661 aaaaaaaaaa agaaatgctt gaactaaagc ttcccctatg ctggagaagt atgaagaaag
     6721 atatacctgg agacattaga acagcgatgg gaaccattgc aactcgggtc catctttgta
     6781 acatgctgaa gacaagcaga agcacatgca cgagggacag aggagctact gtgcactgct
     6841 gtgaaattgc ccagagcata aaacctgtgt accctccttc acatcaacca agttcactag
     6901 gacataccaa gcacatgcgt gtgaatgagg atgcaaggca agagaatgga actccagaat
     6961 tcacagatag aacaaatgga tgagaaatat ttcatgcaaa atataaagtg tcctgaggat
     7021 ctgggttcca tttacgagag gaaatgaaag tgctaaaata aattttatct tccttttaaa
     7081 tgtcagcatg tcagcagaag cagcacacaa aagtctttac cattttccag tattaatttt
     7141 tttgtaatat aaatgataaa ggagatgata aagaaccaat agattattga taaataaatt
     7201 agtaataata tgattttttg tttctatgag ttctaaacag ccctatacag tattcagttt
     7261 ccatgagaaa tatttattgt atcaaagtac attgtactta acattttagg ccatcctttt
     7321 actgttcctt gatgctttaa tatatattaa tttataatta tcctgatatt tttgtacatt
     7381 tttcaaactt aaaaatcagg attttttttt tttttttttt ggcaatagta ctaacatagg
     7441 gttttatctt gggatacata tgtattggtc tgtttgttga ccttgacatc tgatcactga
     7501 tgtcaatgac ctactggcct cattcaggac acctgcagag agtatgcaaa gtccgagaga
     7561 ggaaaacaga aatctgtatg tgagatggtc attgtacaga aagaagtggc ccctctgcaa
     7621 catgtcctca cagaaacgaa atggtgtgta gcaatcaaca ctagaaagta gaccttttgc
     7681 aaattaatat gtccttgacc ttttttgccc ttttgtgggg gtgaggtggg gataaaaaga
     7741 ctgtcatatc aagaactgtg acttttcttt ccctcaaaca ataaaactcc tttattatct
     7801 taatgctccc atgttaacat gtttgctgct aaattacaat gtagaattga taatggttta
     7861 tagtgaactg tgctcttccc tcattaaaat cccagggtgc cctgtaaaga tgcagatgtt
     7921 tcttcctgaa aacttctttt tttacaaaga aaattagatg tacatgtata attcagtgtg
     7981 ctttgtcttt ctccagatta atatcggtta cactgctgat gtttgtaaat taaacagata
     8041 tttacttcat taa
//
