LOCUS       NM_018042               2506 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens schlafen family member 12 (SLFN12), transcript variant
            1, mRNA.
ACCESSION   NM_018042
VERSION     NM_018042.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Kobayashi-Ishihara,M., Frazao Smutna,K., Alonso,F.E.,
            Argilaguet,J., Esteve-Codina,A., Geiger,K., Genesca,M.,
            Grau-Exposito,J., Duran-Castells,C., Rogenmoser,S., Bottcher,R.,
            Jungfleisch,J., Oliva,B., Martinez,J.P., Li,M., David,M.,
            Yamagishi,M., Ruiz-Riol,M., Brander,C., Tsunetsugu-Yokota,Y.,
            Buzon,M.J., Diez,J. and Meyerhans,A.
  TITLE     Schlafen 12 restricts HIV-1 latency reversal by a codon-usage
            dependent post-transcriptional block in CD4+ T cells
  JOURNAL   Commun Biol 6 (1), 487 (2023)
   PUBMED   37165099
  REMARK    GeneRIF: Schlafen 12 restricts HIV-1 latency reversal by a
            codon-usage dependent post-transcriptional block in CD4+ T cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Yan,B., Ding,Z., Zhang,W., Cai,G., Han,H., Ma,Y., Cao,Y., Wang,J.,
            Chen,S. and Ai,Y.
  TITLE     Multiple PDE3A modulators act as molecular glues promoting
            PDE3A-SLFN12 interaction and induce SLFN12 dephosphorylation and
            cell death
  JOURNAL   Cell Chem Biol 29 (6), 958-969 (2022)
   PUBMED   35104454
REFERENCE   3  (bases 1 to 2506)
  AUTHORS   Garvie,C.W., Wu,X., Papanastasiou,M., Lee,S., Fuller,J.,
            Schnitzler,G.R., Horner,S.W., Baker,A., Zhang,T., Mullahoo,J.P.,
            Westlake,L., Hoyt,S.H., Toetzl,M., Ranaghan,M.J., de Waal,L.,
            McGaunn,J., Kaplan,B., Piccioni,F., Yang,X., Lange,M.,
            Tersteegen,A., Raymond,D., Lewis,T.A., Carr,S.A., Cherniack,A.D.,
            Lemke,C.T., Meyerson,M. and Greulich,H.
  TITLE     Structure of PDE3A-SLFN12 complex reveals requirements for
            activation of SLFN12 RNase
  JOURNAL   Nat Commun 12 (1), 4375 (2021)
   PUBMED   34272366
  REMARK    GeneRIF: Structure of PDE3A-SLFN12 complex reveals requirements for
            activation of SLFN12 RNase.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2506)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 2506)
  AUTHORS   Chaturvedi,L.S., Wang,Q., More,S.K., Vomhof-DeKrey,E.E. and
            Basson,M.D.
  TITLE     Schlafen 12 mediates the effects of butyrate and repetitive
            mechanical deformation on intestinal epithelial differentiation in
            human Caco-2 intestinal epithelial cells
  JOURNAL   Hum Cell 32 (3), 240-250 (2019)
   PUBMED   30875077
  REMARK    GeneRIF: Schlafen 12 (SLFN12) is a cytosolic protein that
            stimulates sucrase-isomaltase (SI) expression.
REFERENCE   6  (bases 1 to 2506)
  AUTHORS   Al-Marsoummi,S., Vomhof-DeKrey,E. and Basson,M.D.
  TITLE     Schlafen12 Reduces the Aggressiveness of Triple Negative Breast
            Cancer through Post-Transcriptional Regulation of ZEB1 That Drives
            Stem Cell Differentiation
  JOURNAL   Cell Physiol Biochem 53 (6), 999-1014 (2019)
   PUBMED   31838790
  REMARK    GeneRIF: SLFN12 overexpression reduced MDA-MB-231 mammosphere
            formation. SLFN12 overexpression decreased ZEB1 and Slug protein
            despite increased ZEB1 and Slug mRNA .  SLFN12 knockdown increased
            ZEB1 protein. Co-expressing ZEB1 attenuated the SLFN12 effect on
            E-cadherin mRNA and proliferation.
REFERENCE   7  (bases 1 to 2506)
  AUTHORS   Basson,M.D., Wang,Q., Chaturvedi,L.S., More,S., Vomhof-DeKrey,E.E.,
            Al-Marsoummi,S., Sun,K., Kuhn,L.A., Kovalenko,P. and Kiupel,M.
  TITLE     Schlafen 12 Interaction with SerpinB12 and Deubiquitylases Drives
            Human Enterocyte Differentiation
  JOURNAL   Cell Physiol Biochem 48 (3), 1274-1290 (2018)
   PUBMED   30045019
  REMARK    GeneRIF: SLFN12 regulates human enterocytic differentiation by a
            pathway involving SERPB12, the deubiquitylases, and Cdx2.
REFERENCE   8  (bases 1 to 2506)
  AUTHORS   Nazir,M., Senkowski,W., Nyberg,F., Blom,K., Edqvist,P.H.,
            Jarvius,M., Andersson,C., Gustafsson,M.G., Nygren,P., Larsson,R.
            and Fryknas,M.
  TITLE     Targeting tumor cells based on Phosphodiesterase 3A expression
  JOURNAL   Exp Cell Res 361 (2), 308-315 (2017)
   PUBMED   29107068
  REMARK    GeneRIF: Vulnerability to antineoplastic phosphodiesterase
            inhibitors inhibitors has been associated with co-expression of
            PDE3A and SLFN12 in various tumor types.
REFERENCE   9  (bases 1 to 2506)
  AUTHORS   Kovalenko,P.L. and Basson,M.D.
  TITLE     Schlafen 12 expression modulates prostate cancer cell
            differentiation
  JOURNAL   J Surg Res 190 (1), 177-184 (2014)
   PUBMED   24768141
  REMARK    GeneRIF: Ad-SLFN12 overexpression increased the ratio of the mature
            E-cadherin protein to its precursor protein
REFERENCE   10 (bases 1 to 2506)
  AUTHORS   Schwarz,D.A., Katayama,C.D. and Hedrick,S.M.
  TITLE     Schlafen, a new family of growth regulatory genes that affect
            thymocyte development
  JOURNAL   Immunity 9 (5), 657-668 (1998)
   PUBMED   9846487
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC035605.1, AK001122.1 and CB045471.1.
            
            On Jun 2, 2019 this sequence version replaced NM_018042.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK001122.1, BC035605.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000304905.10/ ENSP00000302077.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-75                BC035605.1         25-99
            76-1225             AK001122.1         76-1225
            1226-2359           BC035605.1         1250-2383
            2360-2506           CB045471.1         278-424
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q12"
     gene            1..2506
                     /gene="SLFN12"
                     /gene_synonym="SLFN3"
                     /note="schlafen family member 12"
                     /db_xref="GeneID:55106"
                     /db_xref="HGNC:HGNC:25500"
                     /db_xref="MIM:614955"
     exon            1..313
                     /gene="SLFN12"
                     /gene_synonym="SLFN3"
                     /inference="alignment:Splign:2.1.0"
     exon            314..1392
                     /gene="SLFN12"
                     /gene_synonym="SLFN3"
                     /inference="alignment:Splign:2.1.0"
     CDS             354..2090
                     /gene="SLFN12"
                     /gene_synonym="SLFN3"
                     /codon_start=1
                     /product="ribonuclease SLFN12"
                     /protein_id="NP_060512.3"
                     /db_xref="CCDS:CCDS11295.1"
                     /db_xref="GeneID:55106"
                     /db_xref="HGNC:HGNC:25500"
                     /db_xref="MIM:614955"
                     /translation="MNISVDLETNYAELVLDVGRVTLGENSRKKMKDCKLRKKQNESV
                     SRAMCALLNSGGGVIKAEIENEDYSYTKDGIGLDLENSFSNILLFVPEYLDFMQNGNY
                     FLIFVKSWSLNTSGLRITTLSSNLYKRDITSAKVMNATAALEFLKDMKKTRGRLYLRP
                     ELLAKRPCVDIQEENNMKALAGVFFDRTELDRKEKLTFTESTHVEIKNFSTEKLLQRI
                     KEILPQYVSAFANTDGGYLFIGLNEDKEIIGFKAEMSDLDDLEREIEKSIRKMPVHHF
                     CMEKKKINYSCKFLGVYDKGSLCGYVCALRVERFCCAVFAKEPDSWHVKDNRVMQLTR
                     KEWIQFMVEAEPKFSSSYEEVISQINTSLPAPHSWPLLEWQRQRHHCPGLSGRITYTP
                     ENLCRKLFLQHEGLKQLICEEMDSVRKGSLIFSRSWSVDLGLQENHKVLCDALLISQD
                     SPPVLYTFHMVQDEEFKGYSTQTALTLKQKLAKIGGYTKKVCVMTKIFYLSPEGMTSC
                     QYDLRSQVIYPESYYFTRRKYLLKALFKALKRLKSLRDQFSFAENLYQIIGIDCFQKN
                     DKKMFKSCRRLT"
     exon            1393..1500
                     /gene="SLFN12"
                     /gene_synonym="SLFN3"
                     /inference="alignment:Splign:2.1.0"
     exon            1501..2506
                     /gene="SLFN12"
                     /gene_synonym="SLFN3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2336..2341
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLFN12"
                     /gene_synonym="SLFN3"
                     /note="hexamer: AATAAA"
     polyA_site      2368
                     /gene="SLFN12"
                     /gene_synonym="SLFN3"
                     /note="major polyA site"
ORIGIN      
        1 attgatcgct ttctgcaacc attcagactg atctcgggct cctatttcat ttacattgtg
       61 tgcacaccaa gtaaccagtg ggaaaacttt agagggtact taaaccccag aaaattctga
      121 aaccgggctc ttgagccgct atcctcgggc ctgctcccac cctgtggagt gcactttcgt
      181 tttcaataaa tctctgcttt tgttgcttca ttctttcctt gctttgtttg tgtgtttgtc
      241 cagttctttg ttcaacacgc caagaacctg gacactcttc actggtaaca tattttggca
      301 agccaaccag gagaaaagaa tttctgcttg gacactgcat agctgctggg aaaatgaaca
      361 tcagtgttga tttggaaacg aattatgccg agttggttct agatgtggga agagtcactc
      421 ttggagagaa cagtaggaaa aaaatgaagg attgtaaact gagaaaaaag cagaatgaaa
      481 gtgtctcacg agctatgtgt gctctgctca attctggagg gggagtgatc aaggctgaaa
      541 ttgagaatga agactatagt tatacaaaag atggaatagg actagatttg gaaaattctt
      601 ttagtaacat tctgttattt gttcctgagt acttagactt catgcagaat ggtaactact
      661 ttctgatttt tgtgaagtca tggagcttga acacctctgg tctgcggatt accaccttga
      721 gctccaattt gtacaaaaga gatataacat ctgcaaaagt catgaatgcc actgctgcac
      781 tggagttcct caaagacatg aaaaagacta gagggagatt gtatttaaga ccagaattgc
      841 tggcaaagag gccctgtgtt gatatacaag aagaaaataa catgaaggcc ttggccgggg
      901 ttttttttga tagaacagaa cttgatcgga aagaaaaatt gacctttact gaatccacac
      961 atgttgaaat taaaaacttc tcgacagaaa agttgttaca acgaattaaa gagattctcc
     1021 ctcaatatgt ttctgcattt gcaaatactg atggaggata tttgttcatt ggtttaaatg
     1081 aagataaaga aataattggc tttaaagcag agatgagtga cctcgatgac ttagaaagag
     1141 aaatcgaaaa gtccattagg aagatgcctg tgcatcactt ctgtatggag aagaagaaga
     1201 taaattattc atgcaaattc cttggagtat atgataaagg aagtctttgt ggatatgtct
     1261 gtgcactcag agtggagcgc ttctgctgtg cagtgtttgc taaagagcct gattcctggc
     1321 atgtgaaaga taaccgtgtg atgcagttga ccaggaagga atggatccag ttcatggtgg
     1381 aggctgaacc aaaattttcc agttcatatg aagaggtgat ctctcaaata aatacgtcat
     1441 tacctgctcc ccacagttgg cctcttttgg aatggcaacg gcagagacat cactgtccag
     1501 ggctatcagg aaggataacg tatactccag aaaacctttg cagaaaactg ttcttacaac
     1561 atgaaggact taagcaatta atatgtgaag aaatggactc tgtcagaaag ggctcactga
     1621 tcttctctag gagctggtct gtggatctgg gcttgcaaga gaaccacaaa gtcctctgtg
     1681 atgctcttct gatttcccag gacagtcctc cagtcctata caccttccac atggtacagg
     1741 atgaggagtt taaaggctat tctacacaaa ctgccctaac cttaaagcag aagctggcaa
     1801 aaattggtgg ttacactaaa aaagtgtgtg tcatgacaaa gatcttctac ttgagccctg
     1861 aaggcatgac aagctgccag tatgatttaa ggtcgcaagt aatttaccct gaatcctact
     1921 attttacaag aaggaaatac ttgctgaaag ccctttttaa agccttaaag agactcaagt
     1981 ctctgagaga ccagttttcc tttgcagaaa atctatacca gataatcggt atagattgct
     2041 ttcagaagaa tgataaaaag atgtttaaat cttgtcgaag gctcacctga tggaaaatgg
     2101 actgggctac tgagatattt ttcattatat atttgataac attctctaat tctgtgaaaa
     2161 tatttctttg aaaactttgc aagttaagca acttaatgtg atgttggata attgggtttt
     2221 gtctattttc acttctccct aaataatctt cacagatatt gtttgaggga tattaggaaa
     2281 attaatttgt taactcgtct gtgcacagta ttatttactc tgtctgtagt tcctgaataa
     2341 attttcttcc atgcttgaac tgggaaaatt gcaacacttt tattcttaat gacaacagtg
     2401 aaaatctccc agcatatacc tagaaaacaa ttataactta caaaagatta tccttgatga
     2461 aactcagaat ttccacagtg ggaatgaata agaaggcaaa actcat
//
