LOCUS       NM_178833               6351 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens solute carrier family 9 member B2 (SLC9B2), transcript
            variant 1, mRNA.
ACCESSION   NM_178833
VERSION     NM_178833.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Velazquez,D., Prusa,V., Masrati,G., Yariv,E., Sychrova,H.,
            Ben-Tal,N. and Zimmermannova,O.
  TITLE     Allosteric links between the hydrophilic N-terminus and
            transmembrane core of human Na+ /H+ antiporter NHA2
  JOURNAL   Protein Sci 31 (12), e4460 (2022)
   PUBMED   36177733
  REMARK    GeneRIF: Allosteric links between the hydrophilic N-terminus and
            transmembrane core of human Na[+] /H[+] antiporter NHA2.
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 6351)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 6351)
  AUTHORS   Uzdavinys,P., Coincon,M., Nji,E., Ndi,M., Winkelmann,I., von
            Ballmoos,C. and Drew,D.
  TITLE     Dissecting the proton transport pathway in electrogenic Na+/H+
            antiporters
  JOURNAL   Proc Natl Acad Sci U S A 114 (7), E1101-E1110 (2017)
   PUBMED   28154142
  REMARK    GeneRIF: The exchange activity of NHA2 (SLC9B2) is electroneutral,
            despite harboring the 2 conserved aspartic acid residues found in
            NapA and other bacterial homologues. The equivalent residue to K305
            in human NHA2 has been replaced with arginine, which is a mutation
            that makes NapA electroneutral. A transmembrane embedded lysine
            residue is essential for electrogenic transport in Na(+)/H(+)
            antiporters.
REFERENCE   5  (bases 1 to 6351)
  AUTHORS   Landreh,M., Marklund,E.G., Uzdavinys,P., Degiacomi,M.T.,
            Coincon,M., Gault,J., Gupta,K., Liko,I., Benesch,J.L., Drew,D. and
            Robinson,C.V.
  TITLE     Integrating mass spectrometry with MD simulations reveals the role
            of lipids in Na+/H+ antiporters
  JOURNAL   Nat Commun 8, 13993 (2017)
   PUBMED   28071645
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6351)
  AUTHORS   Schushan,M., Xiang,M., Bogomiakov,P., Padan,E., Rao,R. and
            Ben-Tal,N.
  TITLE     Model-guided mutagenesis drives functional studies of human NHA2,
            implicated in hypertension
  JOURNAL   J Mol Biol 396 (5), 1181-1196 (2010)
   PUBMED   20053353
  REMARK    GeneRIF: cluster of essential, highly conserved titratable residues
            located in an assembly region made of two discontinuous helices of
            inverted topology, each interrupted by an extended chain
REFERENCE   7  (bases 1 to 6351)
  AUTHORS   Fuster,D.G., Zhang,J., Shi,M., Bobulescu,I.A., Andersson,S. and
            Moe,O.W.
  TITLE     Characterization of the sodium/hydrogen exchanger NHA2
  JOURNAL   J Am Soc Nephrol 19 (8), 1547-1556 (2008)
   PUBMED   18508966
  REMARK    GeneRIF: restricted to the distal convoluted tubule in the kidney
REFERENCE   8  (bases 1 to 6351)
  AUTHORS   Ha,B.G., Hong,J.M., Park,J.Y., Ha,M.H., Kim,T.H., Cho,J.Y.,
            Ryoo,H.M., Choi,J.Y., Shin,H.I., Chun,S.Y., Kim,S.Y. and Park,E.K.
  TITLE     Proteomic profile of osteoclast membrane proteins: identification
            of Na+/H+ exchanger domain containing 2 and its role in osteoclast
            fusion
  JOURNAL   Proteomics 8 (13), 2625-2639 (2008)
   PUBMED   18600791
REFERENCE   9  (bases 1 to 6351)
  AUTHORS   Battaglino,R.A., Pham,L., Morse,L.R., Vokes,M., Sharma,A.,
            Odgren,P.R., Yang,M., Sasaki,H. and Stashenko,P.
  TITLE     NHA-oc/NHA2: a mitochondrial cation-proton antiporter selectively
            expressed in osteoclasts
  JOURNAL   Bone 42 (1), 180-192 (2008)
   PUBMED   17988971
  REMARK    GeneRIF: NHA-oc/NHA2 displays the expected activities of a bona
            fide cation-proton antiporter and plays a key role(s) in normal
            osteoclast differentiation and function
REFERENCE   10 (bases 1 to 6351)
  AUTHORS   Xiang,M., Feng,M., Muend,S. and Rao,R.
  TITLE     A human Na+/H+ antiporter sharing evolutionary origins with
            bacterial NhaA may be a candidate gene for essential hypertension
  JOURNAL   Proc Natl Acad Sci U S A 104 (47), 18677-18681 (2007)
   PUBMED   18000046
  REMARK    GeneRIF: Na+/H+ antiporter genes may contribute to sodium-lithium
            countertransport activity and salt homeostasis in humans[NHA1 and
            NHA2]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC097485.2.
            
            On May 20, 2019 this sequence version replaced NM_178833.6.
            
            Summary: Sodium hydrogen antiporters, such as NHEDC2, convert the
            proton motive force established by the respiratory chain or the
            F1F0 mitochondrial ATPase into sodium gradients that drive other
            energy-requiring processes, transduce environmental signals into
            cell responses, or function in drug efflux (Xiang et al., 2007
            [PubMed 18000046]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.40051.1,
                                           SRR1660807.16302.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            MANE Ensembl match                 :: ENST00000394785.9/
                                                  ENSP00000378265.3
            RefSeq Select criteria             :: based on manual assertion,
                                                  conservation, expression,
                                                  longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-578               AC097485.2         56896-57473         c
            579-710             AC097485.2         48173-48304         c
            711-891             AC097485.2         47039-47219         c
            892-1062            AC097485.2         38513-38683         c
            1063-1205           AC097485.2         30952-31094         c
            1206-1333           AC097485.2         29605-29732         c
            1334-1509           AC097485.2         27763-27938         c
            1510-1616           AC097485.2         25602-25708         c
            1617-1766           AC097485.2         24008-24157         c
            1767-1875           AC097485.2         12412-12520         c
            1876-2012           AC097485.2         9459-9595           c
            2013-6351           AC097485.2         2965-7303           c
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q24"
     gene            1..6351
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /note="solute carrier family 9 member B2"
                     /db_xref="GeneID:133308"
                     /db_xref="HGNC:HGNC:25143"
                     /db_xref="MIM:611789"
     exon            1..578
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /inference="alignment:Splign:2.1.0"
     exon            579..710
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             621..2234
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mitochondrial sodium/hydrogen exchanger NHA2; NHE
                     domain-containing protein 2; mitochondrial Na(+)/H(+)
                     exchanger NHA2; sodium/hydrogen exchanger-like
                     domain-containing protein 2; solute carrier family 9,
                     subfamily B (NHA2, cation proton antiporter 2), member 2;
                     Na(+)/H(+) exchanger-like domain-containing protein 2;
                     Na+/H+ exchanger domain containing 2; solute carrier
                     family 9 subfamily B member 2; mitochondrial
                     sodium/hydrogen exchanger 9B2; solute carrier family 9,
                     subfamily B (cation proton antiporter 2), member 2"
                     /codon_start=1
                     /product="sodium/hydrogen exchanger 9B2 isoform 1"
                     /protein_id="NP_849155.2"
                     /db_xref="CCDS:CCDS3662.1"
                     /db_xref="GeneID:133308"
                     /db_xref="HGNC:HGNC:25143"
                     /db_xref="MIM:611789"
                     /translation="MGDEDKRITYEDSEPSTGMNYTPSMHQEAQEETVMKLKGIDANE
                     PTEGSILLKSSEKKLQETPTEANHVQRLRQMLACPPHGLLDRVITNVTIIVLLWAVVW
                     SITGSECLPGGNLFGIIILFYCAIIGGKLLGLIKLPTLPPLPSLLGMLLAGFLIRNIP
                     VINDNVQIKHKWSSSLRSIALSIILVRAGLGLDSKALKKLKGVCVRLSMGPCIVEACT
                     SALLAHYLLGLPWQWGFILGFVLGAVSPAVVVPSMLLLQGGGYGVEKGVPTLLMAAGS
                     FDDILAITGFNTCLGIAFSTGSTVFNVLRGVLEVVIGVATGSVLGFFIQYFPSRDQDK
                     LVCKRTFLVLGLSVLAVFSSVHFGFPGSGGLCTLVMAFLAGMGWTSEKAEVEKIIAVA
                     WDIFQPLLFGLIGAEVSIASLRPETVGLCVATVGIAVLIRILTTFLMVCFAGFNLKEK
                     IFISFAWLPKATVQAAIGSVALDTARSHGEKQLEDYGMDVLTVAFLSILITAPIGSLL
                     IGLLGPRLLQKVEHQNKDEEVQGETSVQV"
     exon            711..891
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1062
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1205
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1333
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1509
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1510..1616
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1766
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1767..1875
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1876..2012
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2013..6351
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3087..3092
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /note="hexamer: AATAAA"
     polyA_site      3114
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /note="major polyA site"
     regulatory      4033..4038
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /note="hexamer: AATAAA"
     polyA_site      4059
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
     regulatory      6325..6330
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
                     /note="hexamer: AATAAA"
     polyA_site      6351
                     /gene="SLC9B2"
                     /gene_synonym="NHA2; NHE10; NHEDC2"
ORIGIN      
        1 atctccggga agactcggcg cctgggtccg cgctctctgg gtaagctttc cgggaagctt
       61 tcccgggagc tcgctggtcc tggccccaga agcctgcgga cccgcccagg gaggataagc
      121 agctgaaaga ccgcgcggtg ccgctccgag gccccgggac gtgggcccat ggtcggcctg
      181 gcgccacctt tccgggggaa gccacgcgca ccaggcatcg cacgcggctc tgcacccgcg
      241 ccgccggacc tgaaacccgg cggagggcac acggggctgc cgctgcgggc cccggaccaa
      301 cccatgctta ctccggagcc tgtaccggcg ccgacgggtc ggacctccct gcgcggtgtc
      361 gcccagcggg ttcgtgcgaa aggcggggcc gactacacgc ggtgccgcgc cctgagaccg
      421 tttatctgca gtcaacgcag cctcccggct cagcctggga agatgcgcga atcgggaacc
      481 ccagagcgcg gtggctagac cgggctccgc cgcctccccc acagcccctt tcctaatcgt
      541 tcagacggag cctggtcgac ttcgccggag actgccagat ctcgttcctc ttccctgtgt
      601 catcttctta attataaata atgggggatg aagataaaag aattacatat gaagattcag
      661 aaccatccac aggaatgaat tacacgccct ccatgcatca agaagcacag gaggagacag
      721 ttatgaagct caaaggtata gatgcaaatg aaccaacaga aggaagtatt cttttgaaaa
      781 gcagtgaaaa aaagctacaa gaaacaccaa ctgaagcaaa tcacgtacaa agactgagac
      841 aaatgctggc ttgccctcca catggtttac tggacagggt cataacaaat gttaccatca
      901 ttgttcttct gtgggctgta gtttggtcaa ttactggcag tgaatgtctt cctggaggaa
      961 acctatttgg aattataatc ctattctatt gtgccatcat tggtggtaaa cttttggggc
     1021 ttattaagtt acctacattg cctccactgc cttctcttct tggcatgctg cttgcagggt
     1081 ttctcatcag aaatatccca gtcatcaacg ataatgtgca gatcaagcac aagtggtctt
     1141 cctctttgag aagcatagcc ctgtctatca ttctggttcg tgctggcctt ggtctggatt
     1201 caaaggccct gaagaagtta aagggcgttt gtgtaagact gtccatgggt ccctgtattg
     1261 tggaggcgtg cacatctgct cttcttgccc attacctgct gggtttacca tggcaatggg
     1321 gatttatact gggttttgtt ttaggtgctg tatctccagc tgttgtggtg ccttcaatgc
     1381 tccttttgca gggaggaggc tatggtgttg agaagggtgt cccaaccttg ctcatggcag
     1441 ctggcagctt cgatgacatt ctggccatca ctggcttcaa cacatgcttg ggcatagcct
     1501 tttccacagg ctctactgtc tttaatgtcc tcagaggagt tttggaggtg gtaattggtg
     1561 tggcaactgg atctgttctt ggatttttca ttcagtactt tccaagccgt gaccaggaca
     1621 aacttgtgtg taagagaaca ttccttgtgt tggggttgtc tgtgctagct gtgttcagca
     1681 gtgtgcattt tggtttccct ggatcaggag gactgtgcac gttggtcatg gctttccttg
     1741 caggcatggg atggaccagc gaaaaggcag aggttgaaaa gataattgca gttgcctggg
     1801 acatttttca gccccttctt tttggactaa ttggagcaga ggtatctatt gcatctctca
     1861 gaccagaaac tgtaggcctt tgtgttgcca ccgtaggcat tgcagtattg atacgaattt
     1921 tgactacatt tctgatggtg tgttttgctg gttttaactt aaaagaaaag atatttattt
     1981 cttttgcatg gcttccaaag gccacagttc aggctgcaat aggatctgtg gctttggaca
     2041 cagcaaggtc acatggagag aaacaattag aagactatgg aatggatgtg ttgacagtgg
     2101 catttttgtc catcctcatc acagccccaa ttggaagtct gcttattggt ttactgggcc
     2161 ccaggcttct gcagaaagtt gaacatcaaa ataaagatga agaagttcaa ggagagactt
     2221 ctgtgcaagt ttagaggtga aaagagagag tgctgaacat aatgtttaga aagctgctac
     2281 ttttttcaag atgcatattg aaatatgtaa tgtttaagct taaaatgtaa tagaaccaaa
     2341 agtgtagctg tttctttaaa cagcattttt agcccttgct ctttccatgt gggtggtaat
     2401 gatctatatc accaacctta atctctctgc cttttttttc aaacacccct tcatcatcca
     2461 tcttaatttg cataaggaca tatctacttt aatgtactac cacagtttac agttaatgtg
     2521 ggaaagacca gcttcagtat cctcttcagc taggattgcc ctaactttta actttcacag
     2581 tttcctgatt catatttgcc caggctctga tgccttgaat tggttttggc tctctttttt
     2641 ggatctgttt ttgttgttaa acatcataat gcagtctctc attaattttt accatcattt
     2701 accctgataa tctgcctctt ctccatttct ccttccctta ctacctttct ttgaattact
     2761 gtaactgatt ggtcccacca aaattttaaa gtacatgaag tatcttcatt ggttcatcct
     2821 cttgccccct ccagatgtca aaaaacttta tcctgccccc tagctgacca cccaggttcc
     2881 tttatttcag tggcccatgt gagtctacct tcccctaagg agtgccctaa tccagccctt
     2941 tttttgtttc ttatgaccca tatctttagg ctcttcccat ttctaggtgg gagataggta
     3001 agtttcaaat ctatgccagt cttatgaata ttacattagg gtaatgtgct ataatgaaga
     3061 aataaaaaat acagtgctta aaagaaaata aaattctatt tctgtctaat gaaactggct
     3121 ggttcgttaa taccaacagg gttactctgc ttatgaggtc tgctcagagt cccatgtctt
     3181 tctgtcctga agctctgcca ttccctagct taggacatgg cttaacctat ttatgccaga
     3241 ggttgcaaaa attttttgtg aaaaatcaga ccttggcaat gaccttgagc agtgggatat
     3301 aaataactcc cacaggctta gcattctaat aatggaacac taggcataaa tgggttaaaa
     3361 atggatctct accatatctc cattctatct ctcaaggaaa atgcaagtat agcaatttat
     3421 ttttgaggaa gtgatgtgga agttggacac atctcttttc ctacatttat tggtattgat
     3481 catatccggc tgtgaggcag ctaagtgttg tctctagcta agcattcaca ctcctaggag
     3541 gaacggaaca tcagattttc ggggccagag cttccactgt gttggatgtt accatcattc
     3601 agtagcatat tcttacatgc aaacaaaagc ttttgaaaga cattgcctta aaccaaaaga
     3661 gtgacttcta tggcagggat ttcagaaatt gtgacttgcc agcagagaaa atattagaag
     3721 aaagacttac ctgcataggt catattttat cctactttgt aacaggccat ctctgctttt
     3781 gtgtatcact cataagttcc tcttcactct aggtcatctt ctgtgtagtt actgaaataa
     3841 tcacattcac atacagttta cagtttttat catgttatct gtgtacctga agtgtttatt
     3901 acatacttgt catatcctat ctaaactcct cgacctagaa ttcaaagttc attttaatct
     3961 tgaagtagat attaaatatg tattagatga atgaattatg aatatcctaa tcccaccaag
     4021 ctgatctgca taaataaagc ctgtccattc ctctcttcat tagacttctg ttcgagccct
     4081 atccaccatg gctttcacct ctattcaaaa ctagtttaat attcacttct tctaaatagc
     4141 cttcaatttt atgtcaactt tgctttggtt accctatggt gctgtattcc tttaatgttt
     4201 atgctcttga tttggacttg attaatttat accaaaggtc agataagtgt tttgaactga
     4261 acttcattgg ttaggtgcac tttgctggga gcaccataaa cttaatgatc atctggtcct
     4321 ctatttttgc aggctctaaa agtagatgca tccaaaaaat acttactgaa agcttttgag
     4381 atccaggcac atcatcaaga ataatattgt ccctgtcctt aaggatttaa cagtccaata
     4441 gagaaaacag acagttaaac aagacattgc aaaacaatat gataagtgct attatgcagg
     4501 aactacaggg ctgctaggga gctcatggca ggagcactca atcaatccaa gggtggtgga
     4561 gtttaggcac agcttcccag agaaagtagg gcattggctg agggaagatt aggacttagc
     4621 cagcaaaagg gatgtaagac caaaaagtgt tacacttcat gctgtttccc aggtcatcag
     4681 tggcattttc tccaagtaac aggcttggaa tatttattaa cttatacata atacagttag
     4741 tataactaaa tcagaatgct attgaatatg tttagggccc ctaggaaaat tagaaatgga
     4801 agtccacaga gtacagaaat gattgaaggg cacctgaagc cactgccaat acctgttttc
     4861 attttgaggt catgcagcaa actcttttct acctctaaga gggtagccac catctaaatc
     4921 tctgtgactg cccagtgctg gctttttgac ttcctctatt ggatctctga aatttgtgcc
     4981 tattcctgga agctctttgt aggaatttat taattgctat aagactatgt tgctgttata
     5041 tatcatggag atggtgaggt caagaatgac agaggctgac cattcagcag ggctcaccat
     5101 gtgaaggccg gggtttgtca ccacattgaa agccacatct cttgacttgg ttggtgaact
     5161 tagggttaac ctgatagagt actgaccaac tcaacctatc ccctcttaaa ccacagatag
     5221 aaacaattac tctaactttt gggtccctta tacatactta tgtcagagca catgtaacac
     5281 tttttttcac tttatttaca tgtttgtctc ttattaaatc agctccctat gggtaggaac
     5341 cttgtcttaa ttatccttga atcctcagca tttttcacag ggcatggaac acagtagaca
     5401 tttaataaac atttactgac taaatctgag aggaagagga tcctctctca atatcagaaa
     5461 actacttgcc acacgacagt tcggatctta gtccacttag tctatcttag tcagcttggg
     5521 ctgctataac aaaataccat acactgggtg gcttacacaa cagaaactta cttctattaa
     5581 aggttggaag ttcgagatca ggatgccagc attgttgggc tctggtgaag tctctctccc
     5641 tggcttgcca atggccacat tctcaccctc tcactcatgt gtcatcactt gatgccgaga
     5701 gagagcaagc aagctctctg gtgtctcttc ttataagggt actctaatca cagcatgagg
     5761 gccctaccct catgacctca tccaaatcta attgccttcc caaatcctca tatctaaata
     5821 catcacattg gaagttaagg cttcaacata tgaatgtgtg gggaaggaca taattcagtc
     5881 cctgaaagaa gtgtcatatt tccaaggaca gtatatttct gtctggtatt aactcccaat
     5941 caccttgatt gatcttgatg tcctcaaaat ttcctcaggt aaacatttgc aaatgatctc
     6001 atccactaca ttctttaaaa atagtatttt tttctgatta taaagtaaca cattttcatt
     6061 gcaaaaaatt tagaacacag aaaaatacaa acaagaaaac tgaaactaat ataaacatgt
     6121 attttatttt tctttaaaat tgtactgcag ttttggggga tgtattattt tcagaaagat
     6181 atatcatcaa ctgaatttgt tgggtgaaag gcaacataag tatttcatat ttggctttta
     6241 tgatgctatg tagattgaag aatttttttg tatataatga atgctaagcc ttcatgttta
     6301 tattcagata ttcattttgc aatgaataaa acagacaaat ccctgccttc a
//
