LOCUS       NM_015266               6147 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens solute carrier family 9 member A8 (SLC9A8), transcript
            variant 2, mRNA.
ACCESSION   NM_015266 XM_030524
VERSION     NM_015266.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Liu,W., Geng,C., Li,X., Li,Y., Song,S. and Wang,C.
  TITLE     Downregulation of SLC9A8 Promotes Epithelial-Mesenchymal Transition
            and Metastasis in Colorectal Cancer Cells via the IL6-JAK1/STAT3
            Signaling Pathway
  JOURNAL   Dig Dis Sci 68 (5), 1873-1884 (2023)
   PUBMED   36583805
  REMARK    GeneRIF: Downregulation of SLC9A8 Promotes Epithelial-Mesenchymal
            Transition and Metastasis in Colorectal Cancer Cells via the
            IL6-JAK1/STAT3 Signaling Pathway.
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Zhou,K., Amiri,M., Salari,A., Yu,Y., Xu,H., Seidler,U. and
            Nikolovska,K.
  TITLE     Functional characterization of the sodium/hydrogen exchanger 8 and
            its role in proliferation of colonic epithelial cells
  JOURNAL   Am J Physiol Cell Physiol 321 (3), C471-C488 (2021)
   PUBMED   34288721
  REMARK    GeneRIF: Functional characterization of the sodium/hydrogen
            exchanger 8 and its role in proliferation of colonic epithelial
            cells.
REFERENCE   3  (bases 1 to 6147)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 6147)
  AUTHORS   Oberheide,K., Puchkov,D. and Jentsch,T.J.
  TITLE     Loss of the Na+/H+ exchanger NHE8 causes male infertility in mice
            by disrupting acrosome formation
  JOURNAL   J Biol Chem 292 (26), 10845-10854 (2017)
   PUBMED   28476888
  REMARK    GeneRIF: work has uncovered a crucial role of NHE8 in acrosome
            biogenesis and suggests that some forms of human globozoospermia
            might be caused by a loss of function of this Na(+)/H(+) exchanger.
REFERENCE   5  (bases 1 to 6147)
  AUTHORS   Xu,H., Li,Q., Zhao,Y., Li,J. and Ghishan,F.K.
  TITLE     Intestinal NHE8 is highly expressed in goblet cells and its
            expression is subject to TNF-alpha regulation
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 310 (2), G64-G69 (2016)
   PUBMED   26564720
  REMARK    GeneRIF: NHE8 is expressed in goblet cells, and the inflammatory
            cytokine TNF-alpha downregulates NHE8 expression by a
            transcriptional mechanism.
REFERENCE   6  (bases 1 to 6147)
  AUTHORS   Katzir,Z., Dinour,D., Reznik-Wolf,H., Nissenkorn,A. and Holtzman,E.
  TITLE     Familial pure proximal renal tubular acidosis--a clinical and
            genetic study
  JOURNAL   Nephrol Dial Transplant 23 (4), 1211-1215 (2008)
   PUBMED   17881426
  REMARK    GeneRIF: No mutation was found in the coding regions and
            intron-exon boundaries of the genes for CA II, CA IV, CA XIV,
            kNCB1, NHE3, NHE8, NHRF1, NHRF2 and SLC26A6 amplified from genomic
            DNA of family members with pRTA.
REFERENCE   7  (bases 1 to 6147)
  AUTHORS   Xu,H., Chen,H., Dong,J., Lynch,R. and Ghishan,F.K.
  TITLE     Gastrointestinal distribution and kinetic characterization of the
            sodium-hydrogen exchanger isoform 8 (NHE8)
  JOURNAL   Cell Physiol Biochem 21 (1-3), 109-116 (2008)
   PUBMED   18209477
  REMARK    GeneRIF: NHE8 is expressed along the gastrointestinal tract and
            NHE8 is a functional Na(+)/H(+) exchanger with kinetic
            characteristics that differ from other apically expressed NHE
            isoforms.
REFERENCE   8  (bases 1 to 6147)
  AUTHORS   Nakamura,N., Tanaka,S., Teko,Y., Mitsui,K. and Kanazawa,H.
  TITLE     Four Na+/H+ exchanger isoforms are distributed to Golgi and
            post-Golgi compartments and are involved in organelle pH regulation
  JOURNAL   J Biol Chem 280 (2), 1561-1572 (2005)
   PUBMED   15522866
REFERENCE   9  (bases 1 to 6147)
  AUTHORS   Orlowski,J. and Grinstein,S.
  TITLE     Diversity of the mammalian sodium/proton exchanger SLC9 gene family
  JOURNAL   Pflugers Arch 447 (5), 549-565 (2004)
   PUBMED   12845533
  REMARK    Review article
REFERENCE   10 (bases 1 to 6147)
  AUTHORS   Goyal,S., Vanden Heuvel,G. and Aronson,P.S.
  TITLE     Renal expression of novel Na+/H+ exchanger isoform NHE8
  JOURNAL   Am J Physiol Renal Physiol 284 (3), F467-F473 (2003)
   PUBMED   12409279
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL162615.13, AL031685.18, AB023156.2 and AL049469.1.
            
            On Jun 2, 2019 this sequence version replaced NM_015266.2.
            
            Summary: Sodium-hydrogen exchangers (NHEs), such as SLC9A8, are
            integral transmembrane proteins that exchange extracellular Na+ for
            intracellular H+. NHEs have multiple functions, including
            intracellular pH homeostasis, cell volume regulation, and
            electroneutral NaCl absorption in epithelia (Xu et al., 2008
            [PubMed 18209477]).[supplied by OMIM, Apr 2009].
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the coding region, but maintains the reading frame, compared to
            variant 1. The encoded isoform (2) is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.2616690.1, AB023156.2
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361573.3/ ENSP00000354966.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-121               AL162615.13        106428-106548
            122-303             AL162615.13        108608-108789
            304-384             AL162615.13        116661-116741
            385-443             AL162615.13        133141-133199
            444-527             AL162615.13        138636-138719
            528-629             AL031685.18        3187-3288
            630-664             AL031685.18        4418-4452
            665-808             AL031685.18        9046-9189
            809-947             AL031685.18        16537-16675
            948-1053            AL031685.18        18347-18452
            1054-1170           AL031685.18        28313-28429
            1171-1253           AL031685.18        31589-31671
            1254-1365           AL031685.18        34532-34643
            1366-1501           AL031685.18        37454-37589
            1502-4064           AB023156.2         1449-4011
            4065-6147           AL049469.1         249-2331
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.13"
     gene            1..6147
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /note="solute carrier family 9 member A8"
                     /db_xref="GeneID:23315"
                     /db_xref="HGNC:HGNC:20728"
                     /db_xref="MIM:612730"
     exon            1..121
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..1841
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /note="isoform 2 is encoded by transcript variant 2;
                     Na(+)/H(+) exchanger 8; solute carrier family 9
                     (sodium/hydrogen exchanger), member 8; solute carrier
                     family 9, subfamily A (NHE8, cation proton antiporter 8),
                     member 8"
                     /codon_start=1
                     /product="sodium/hydrogen exchanger 8 isoform 2"
                     /protein_id="NP_056081.1"
                     /db_xref="CCDS:CCDS13421.1"
                     /db_xref="GeneID:23315"
                     /db_xref="HGNC:HGNC:20728"
                     /db_xref="MIM:612730"
                     /translation="MGEKMAEEERFPNTTHEGFNVTLHTTLVVTTKLVLPTPGKPILP
                     VQTGEQAQQEEQSSGMTIFFSLLVLAICIILVHLLIRYRLHFLPESVAVVSLGILMGA
                     VIKIIEFKKLANWKEEEMFRPNMFFLLLLPPIIFESGYSLHKGNFFQNIGSITLFAVF
                     GTAISAFVVGGGIYFLGQADVISKLNMTDSFAFGSLISAVDPVATIAIFNALHVDPVL
                     NMLVFGESILNDAVSIVLTNTAEGLTRKNMSDVSGWQTFLQALDYFLKMFFGSAALGT
                     LTGLISALVLKHIDLRKTPSLEFGMMIIFAYLPYGLAEGISLSGIMAILFSGIVMSHY
                     THHNLSPVTQILMQQTLRTVAFLCETCVFAFLGLSIFSFPHKFEISFVIWCIVLVLFG
                     RAVNIFPLSYLLNFFRDHKITPKMMFIMWFSGLRGAIPYALSLHLDLEPMEKRQLIGT
                     TTIVIVLFTILLLGGSTMPLIRLMDIEDAKAHRRNKKDVNLSKTEKMGNTVESEHLSE
                     LTEEEYEAHYIRRQDLKGFVWLDAKYLNPFFTRRLTQEDLHHGRIQMKTLTNKWYEEV
                     RQGPSGSEDDEQELL"
     exon            122..303
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            304..384
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            385..443
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            444..527
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            528..629
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            630..664
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            665..808
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            809..947
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1053
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1170
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1253
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1254..1365
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..1586
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1587..1733
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1734..6147
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4276..4281
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /note="hexamer: TATAAA"
     polyA_site      4300
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
     regulatory      6122..6127
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /note="hexamer: AATAAA"
     polyA_site      6147
                     /gene="SLC9A8"
                     /gene_synonym="NHE-8; NHE8"
                     /note="major polyA site"
ORIGIN      
        1 gctcgcccgc ccgcgcctcc agcggaagcc ggaagcaaaa gcgggtcctg ctagccccgc
       61 ggctccgaac tcggtggtcc tggaagctcc gcaggatggg ggagaagatg gcggaagagg
      121 agaggttccc caatacaact catgagggtt tcaatgtcac cctccacacc accctggttg
      181 tcacgacgaa actggtgctc ccgacccctg gcaagcccat cctccccgtg cagacagggg
      241 agcaggccca gcaagaggag cagtccagcg gcatgaccat tttcttcagc ctccttgtcc
      301 tagctatctg catcatattg gtgcatttac tgatccgata cagattacat ttcttgccag
      361 agagtgttgc tgttgtttct ttaggtattc tcatgggagc agttataaaa attatagagt
      421 ttaaaaaact ggcgaattgg aaggaagaag aaatgtttcg tccaaacatg tttttcctcc
      481 tcctgcttcc ccctattatc tttgagtctg gatattcatt acacaagggt aacttctttc
      541 aaaatattgg ttccatcacc ctgtttgctg tttttgggac ggcaatctcc gcttttgtag
      601 taggtggagg aatttatttt ctgggtcagg ctgatgtaat ctctaaactc aacatgacag
      661 acagttttgc gtttggctcc ctaatatctg ctgtcgatcc agtggccact attgccattt
      721 tcaatgcact tcatgtggac cccgtgctca acatgctggt ctttggagaa agtattctca
      781 acgatgcagt ctccattgtt ctgaccaaca cagctgaagg tttaacaaga aaaaatatgt
      841 cagatgtcag tgggtggcaa acatttttac aagcccttga ctacttcctc aaaatgttct
      901 ttggctctgc agcgctcggc actctcactg gcttaatttc tgcattagtg ctgaagcata
      961 ttgacttgag gaaaacgcct tccttggagt ttggcatgat gatcattttt gcttatctgc
     1021 cttatgggct tgcagaagga atctcactct caggcatcat ggccatcctt ttctcaggca
     1081 tcgtgatgtc ccactacacg caccataacc tctccccagt cacccagatc ctcatgcagc
     1141 agaccctccg caccgtggcc ttcttatgtg aaacatgtgt gtttgcattt cttggcctgt
     1201 ccatttttag ttttcctcac aagtttgaaa tttcctttgt catctggtgc atagtgcttg
     1261 tactatttgg cagagcggta aacattttcc ctctttccta cctcctgaat ttcttccggg
     1321 atcataaaat cacaccgaag atgatgttca tcatgtggtt tagtggcctg cggggagcca
     1381 tcccctatgc cctgagccta cacctggacc tggagcccat ggagaagcgg cagctcatcg
     1441 gcaccaccac catcgtcatc gtgctcttca ccatcctgct gctgggcggc agcaccatgc
     1501 ccctcattcg cctcatggac atcgaggacg ccaaggcaca ccgcaggaac aagaaggacg
     1561 tcaacctcag caagactgag aagatgggca acactgtgga gtcggagcac ctgtcggagc
     1621 tcacggagga ggagtacgag gcccactaca tcaggcggca ggaccttaag ggcttcgtgt
     1681 ggctggacgc caagtacctg aaccccttct tcactcggag gctgacgcag gaggacctgc
     1741 accacgggcg catccagatg aaaactctca ccaacaagtg gtacgaggag gtacgccagg
     1801 gcccctccgg ctccgaggac gacgagcagg agctgctctg acgccaggtg ccaaggcttc
     1861 aggcaggcag gcccaggatg ggcgtttgct gcgcacagac actcagcagg ggcctcgcag
     1921 agatgcgtgc atccagcagc cccttcaaga cataagaggg cggggcgagg tactggctgc
     1981 agagtcgcct tagtccagaa cctgacaggc ctctggagcc aggcgacttc ttgggaaact
     2041 gtcatctccc gactcctccc tgagccagcc tccgctcagt gtggctcctc agcccacaga
     2101 ggggagggag catggggcca ggtgccagtc atctgtgaag ctagggcgcc taccccccca
     2161 cccggaggac ccctgcggcc ccctgcctag aggagcacca tctacagttg tgccattccc
     2221 cagccactgc cttcatgctg cccccgccgg actggcagag ccaggggtca gccacctgcc
     2281 tttgagtcat caagatgcct ctgcagccac aattctgacc taagtggcag ggcccagaaa
     2341 tcctgaaaac ctcccgctgc cttttgtgat acttcctgtg ctccctcaga gagaaacgga
     2401 gtgacctttt gtcctttacc tgattggcac ttcgcagtct atctccctgg gtagcagacg
     2461 gctgctgccc ttctctgggc atgttctgaa tgtttacact ggtaccttct ggtatcttct
     2521 ttagagcccc ctgcaagctg caactctagg cttttatctt gcggggtcag agcgccctct
     2581 agagggaaaa gctagaggca cagggtttct gccggcccac aactgctgtc ttgatttgca
     2641 ttttacagca aagtgctgag agcctctagt cgcctcctgc catctgatct ccctccccac
     2701 cattcccgta ctcagttgtt cttttgtcta atcggaggcc actgtgctga ggccctgcag
     2761 tgtctgctca ctgctgccat cttcgctgct agtcagggtt ccatcctctt tcccctctcc
     2821 cagttcccta ccacgttgga tcccattcgt cacccatgct agggtcccca aagcactggg
     2881 gcaggggcca gagcagcagc acccagtgct ccctcctcta ctctgacctg gggccccagc
     2941 atcctggagc acacgctcca cgcacacaca ccccagccct gtcccagggg cctggccccc
     3001 tcagccatct cagggtgagg agctgccagt catgtccaga tggaatgact cccatcctct
     3061 cctcatctcc cctttgacga gcctcaaact gctcagctca tcaaagagcc attgccaact
     3121 tccgtatgtg gttctgggtc ccagggagcc ttggaacctg gcaccctggg gtggtttaat
     3181 tcatcattaa gaagcattcc tgcttctcaa gggacacagt ggcctgcatg ggccagcatg
     3241 gaccctgggc tgatcatgtg cattcctgct tctctgggga cacagtgggc ccacatgggc
     3301 cagcatggac cctgggctag agcaagcaca tctccatctc ttccacctca ggcagtgtgg
     3361 ctccagatgt caggagggac tgacctcagg accttccagg ttcctctgtg ccaggaatga
     3421 gaggccaggc ccgatcctac cacctcgcct tgaccctgaa gtcagagcag gccagccaag
     3481 caggaagcac actgtttact ttttgcatga aaagtaaatg tgtacttgat agagctaaaa
     3541 tatgatcttt tttaatttct caaccccata atttgagcca ttgccttgct taattttggt
     3601 ttccaccatt tccttttagt ggagaagaga ggaagtcaga gggtagggac ctttgcctgc
     3661 ccctgggcga gtgcgggcag ggatctgaga ccagattgtt ctcgcacccc tgccagaact
     3721 cactctcccc tgaagtttag ggtcccatct cccagatgta agttgttttg caaactcagt
     3781 ttgccaggat ttctttcttt cctaatctta aattcacaga taaagcaatg aaaagagtca
     3841 gatcccattt ccgtctgccc cctcgtcacc aggtgtgata gccccagcca ggtcacacct
     3901 ggcctcacac tttgagctga gacttgaaaa cgatgctgtg gcggaagagc atgtggggct
     3961 tggtggaggg gccccaggat ttgttggggg caaagggggt ggcgggaccg ttcccaggag
     4021 gtaccagcac ctgcctcgat ctcctctgag cctcttctgc cccctgtcgg ccaggtgagg
     4081 tcagcagcct gggagagtgc ccccaagaga tgagggcacc ccgtgttcct tggcaatctt
     4141 ggctcacctt ggtaacaaaa ggccatagaa gtctgttttt ctgggtcagt tttttttgcc
     4201 tgagaataac aaattgctgc tgtctacctt tagcacaccc aataattcta tttggggcag
     4261 tgaatgcata gaagatataa aaatacgcag cttaactata tcttcctgcg tgtgtattta
     4321 ttttcttctg ggtctaggcc atggtacagg agaactgtgg cgtgtaggag gaatacttca
     4381 ggatgagtga aggctggagc cagggagcgc tggaggaaac cagcccttta gccagcagcc
     4441 cctccaccac aggcactgct gtgtggaacg agttcttgga atgaatccca tgctttctgc
     4501 agcctgtagt tgttatgacc cctcggaaca accaccccgt ggcttgtgtg gggtctcgca
     4561 gggaaaaggg ctggcttcta ggtccccgag ataagtgtgc agggggatgg gccagggcca
     4621 ggctaagggt ggctcagttc catcatctgg aggtcagaca cactgtccag aggcagaact
     4681 gaagccctct cggcccctac cctaagccag ccacccctct tcacagtggg tgagctgggc
     4741 tgggctggct ggcatgaggc caaggggtag gcctgagcgc cagagtcgcc caggttagcc
     4801 cacaggattc ctttgtgtgc catggaatgc tgaaagatgg gtgactgggg acccttctta
     4861 aaacctttgg caaaggtgcc atcggcaggg cttggcctca tgaagtctca ggtccgtgtt
     4921 cccgcagggc gcacatgctt ggagagtcct cagcagggta gccgaggcca ggccacttct
     4981 gctgaggatg gggcaggctg gggtgtgggt gtggcctggg gtggctcagg gctggaactg
     5041 ctgcctgatt cctgtgtggg gagaagctca gtggccgttt gctgccactg acaaggattt
     5101 cacatgcaga agagaaaagg cccccctcca ccccccgcat tccctgccga gtgagagcca
     5161 gtgtttgctg cccttgctgg gggcgggtag gaaaccctga gcttcctgat gcggagtcat
     5221 gaagcagagt cctcgggaag gcatctccac agccccgggt cctctgtcta acgccctcca
     5281 tttcacgccc tccatctcac agtcaagata aaggcctcga gaataaagag ccagccccct
     5341 tccatttagt ctcctgccgt ttcccaaaca gttgtccaac agttagacat tgaggggctt
     5401 cactgttacc aggcatgtaa cagaaggagg aagactaaca cacaccccct gccccatccc
     5461 atccccctct cccgagctat tttcttgctg tggcctctgg tgcccttgag ttggtctccc
     5521 cggctgctct gcgggggctt cactggcttc ggagtgagcg cgaagtgctg gtgagcagtg
     5581 ggcctgtgat tggatgggaa gatgtgcatc cgtggtcaaa agtcagctgc cagccctgcg
     5641 gaaccagagc ctcaggctgg gatggggagg cctccctgct tccacctgca tggtgggcat
     5701 ggcctggctt acaccaaagg ctttgacggt ttctccaagt aaggatctgc aaatcttgaa
     5761 tcgtcctcaa aatgacgaag cttgaattgt cctcaagatg gatgtgaatc ttacattcct
     5821 tttcatcatt tcctttgtaa aaatgacgag tgctgggttt ttgttttaag aagcattatg
     5881 aaggccagac ttactcattt ttctccccca agtgagctgc aagaggcccc tgttaggccc
     5941 ctgtttcctg agcagtgatg tgctgctctt cttggtgggg ctttgggctg ggaggggaag
     6001 gcgggtcaga gatgggggac ctgtggctgc catgcaggag cccctgcgtc atctcgttgg
     6061 actctttaag ggagtcagga atagatgtat gaacagtcgt gtcactggat gcctatttag
     6121 aaataaagtg tatgctgctg aattgga
//
