LOCUS       NM_001257291            9971 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens solute carrier family 9 member A7 (SLC9A7), transcript
            variant 1, mRNA.
ACCESSION   NM_001257291
VERSION     NM_001257291.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9971)
  AUTHORS   Yao,Y., Xu,Y., Yu,L., Xue,T.M., Xiao,Z.J., Tin,P.C., Fung,H.L.,
            Ma,H.T., Yun,J.P. and Yam,J.W.P.
  TITLE     NHE7 upregulation potentiates the uptake of small extracellular
            vesicles by enhancing maturation of macropinosomes in
            hepatocellular carcinoma
  JOURNAL   Cancer Commun (Lond) 44 (2), 251-272 (2024)
   PUBMED   38152992
  REMARK    GeneRIF: NHE7 upregulation potentiates the uptake of small
            extracellular vesicles by enhancing maturation of macropinosomes in
            hepatocellular carcinoma.
REFERENCE   2  (bases 1 to 9971)
  AUTHORS   Khayat,W., Hackett,A., Shaw,M., Ilie,A., Dudding-Byth,T.,
            Kalscheuer,V.M., Christie,L., Corbett,M.A., Juusola,J.,
            Friend,K.L., Kirmse,B.M., Gecz,J., Field,M. and Orlowski,J.
  TITLE     A recurrent missense variant in SLC9A7 causes nonsyndromic X-linked
            intellectual disability with alteration of Golgi acidification and
            aberrant glycosylation
  JOURNAL   Hum Mol Genet 28 (4), 598-614 (2019)
   PUBMED   30335141
  REMARK    GeneRIF: These data implicate a crucial role for SLC9A7 in the
            regulation of TGN/post-Golgi pH homeostasis and glycosylation of
            exported cargo, which may underlie the cellular pathophysiology and
            neurodevelopmental deficits associated with this particular
            nonsyndromic form of X-linked intellectual disability.
REFERENCE   3  (bases 1 to 9971)
  AUTHORS   Obaid,A., Nashabat,M., Al Fakeeh,K., Al Qahtani,A.T. and
            Alfadhel,M.
  TITLE     Delineation of cystinuria in Saudi Arabia: A case series
  JOURNAL   BMC Nephrol 18 (1), 50 (2017)
   PUBMED   28166740
  REMARK    GeneRIF: In a Saudi Arabian cohort of patients with cystinuria, two
            new variants in the SLC3A1 and SLC9A7 genes were discovered. All of
            the detected mutations were missense variants in three different
            exons, such as c.1711 T > A (p.Cys571Ser) (exon 10), c.1166C > T
            p.Thr389Met (exon 11) and c.1400 T > A p.Met467Lys (exon 8).
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9971)
  AUTHORS   Milosavljevic,N., Monet,M., Lena,I., Brau,F., Lacas-Gervais,S.,
            Feliciangeli,S., Counillon,L. and Poet,M.
  TITLE     The intracellular Na(+)/H(+) exchanger NHE7 effects a
            Na(+)-coupled, but not K(+)-coupled proton-loading mechanism in
            endocytosis
  JOURNAL   Cell Rep 7 (3), 689-696 (2014)
   PUBMED   24767989
  REMARK    GeneRIF: NHE7 mediates an acidification of intracellular vesicles
            that is additive to that of V-ATPases and that accelerates
            endocytosis.
REFERENCE   5  (bases 1 to 9971)
  AUTHORS   Onishi,I., Lin,P.J., Numata,Y., Austin,P., Cipollone,J.,
            Roberge,M., Roskelley,C.D. and Numata,M.
  TITLE     Organellar (Na+, K+)/H+ exchanger NHE7 regulates cell adhesion,
            invasion and anchorage-independent growth of breast cancer
            MDA-MB-231 cells
  JOURNAL   Oncol Rep 27 (2), 311-317 (2012)
   PUBMED   22076128
  REMARK    GeneRIF: these results suggest that NHE7 enhances tumor
            progression.
REFERENCE   6  (bases 1 to 9971)
  AUTHORS   Fukura,N., Ohgaki,R., Matsushita,M., Nakamura,N., Mitsui,K. and
            Kanazawa,H.
  TITLE     A membrane-proximal region in the C-terminal tail of NHE7 is
            required for its distribution in the trans-Golgi network, distinct
            from NHE6 localization at endosomes
  JOURNAL   J Membr Biol 234 (3), 149-158 (2010)
   PUBMED   20364249
  REMARK    GeneRIF: The results suggest that two membrane proximal regions
            (residues 533-559 and 563-568) play an important role in targeting
            NHE7 to the TGN
REFERENCE   7  (bases 1 to 9971)
  AUTHORS   Kagami,T., Chen,S., Memar,P., Choi,M., Foster,L.J. and Numata,M.
  TITLE     Identification and biochemical characterization of the SLC9A7
            interactome
  JOURNAL   Mol Membr Biol 25 (5), 436-447 (2008)
   PUBMED   18654930
  REMARK    GeneRIF: Among the NHE7-binding proteins identified, CD44, a cell
            surface glycoprotein receptor for hyaluronate and other ligands,
            showed regulated interaction with NHE7.
REFERENCE   8  (bases 1 to 9971)
  AUTHORS   Lin,P.J., Williams,W.P., Luu,Y., Molday,R.S., Orlowski,J. and
            Numata,M.
  TITLE     Secretory carrier membrane proteins interact and regulate
            trafficking of the organellar (Na+,K+)/H+ exchanger NHE7
  JOURNAL   J Cell Sci 118 (Pt 9), 1885-1897 (2005)
   PUBMED   15840657
  REMARK    GeneRIF: Secretory carrier membrane proteins participate in the
            shuttling of NHE7 between recycling vesicles and the TGN
REFERENCE   9  (bases 1 to 9971)
  AUTHORS   Thiselton,D.L., McDowall,J., Brandau,O., Ramser,J., d'Esposito,F.,
            Bhattacharya,S.S., Ross,M.T., Hardcastle,A.J. and Meindl,A.
  TITLE     An integrated, functionally annotated gene map of the DXS8026-ELK1
            interval on human Xp11.3-Xp11.23: potential hotspot for
            neurogenetic disorders
  JOURNAL   Genomics 79 (4), 560-572 (2002)
   PUBMED   11944989
  REMARK    Erratum:[Genomics 2002 Jun;79(6):891]
REFERENCE   10 (bases 1 to 9971)
  AUTHORS   Numata,M. and Orlowski,J.
  TITLE     Molecular cloning and characterization of a novel (Na+,K+)/H+
            exchanger localized to the trans-Golgi network
  JOURNAL   J Biol Chem 276 (20), 17387-17394 (2001)
   PUBMED   11279194
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC094742.1, AL022165.1 and
            AI081096.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001257291.1.
            
            Summary: This gene encodes a sodium and potassium/ proton
            antiporter that is a member of the solute carrier family 9 protein
            family. The encoded protein is primarily localized to the
            trans-Golgi network and is involved in maintaining pH homeostasis
            in organelles along the secretory and endocytic pathways. This
            protein may enhance cell growth of certain breast tumors. This gene
            is part of a gene cluster on chromosome Xp11.23. A pseudogene of
            this gene is found on chromosome 12. Alternate splicing results in
            multiple transcript variants. [provided by RefSeq, Mar 2012].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.271032.1,
                                           SRR1163658.525925.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000616978.5/ ENSP00000480916.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-853               BC094742.1         55-907
            854-854             AL022165.1         150984-150984       c
            855-1653            BC094742.1         908-1706
            1654-1654           AL022165.1         124636-124636       c
            1655-2248           BC094742.1         1708-2301
            2249-9529           AL022165.1         81042-88322         c
            9530-9971           AI081096.1         17-458              c
FEATURES             Location/Qualifiers
     source          1..9971
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp11.3"
     gene            1..9971
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /note="solute carrier family 9 member A7"
                     /db_xref="GeneID:84679"
                     /db_xref="HGNC:HGNC:17123"
                     /db_xref="MIM:300368"
     exon            1..414
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     CDS             90..2270
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /note="isoform 1 is encoded by transcript variant 1;
                     nonselective sodium potassium/proton exchanger; Na(+)/H(+)
                     exchanger 7; solute carrier family 9, subfamily A (NHE7,
                     cation proton antiporter 7), member 7; solute carrier
                     family 9 (sodium/hydrogen exchanger)"
                     /codon_start=1
                     /product="sodium/hydrogen exchanger 7 isoform 1"
                     /protein_id="NP_001244220.1"
                     /db_xref="CCDS:CCDS75967.1"
                     /db_xref="GeneID:84679"
                     /db_xref="HGNC:HGNC:17123"
                     /db_xref="MIM:300368"
                     /translation="MEPGDAARPGSGRATGAPPPRLLLLPLLLGWGLRVAAAASASSS
                     GAAAEDSSAMEELATEKEAEESHRQDSVSLLTFILLLTLTILTIWLFKHRRVRFLHET
                     GLAMIYGLIVGVILRYGTPATSGRDKSLSCTQEDRAFSTLLVNVSGKFFEYTLKGEIS
                     PGKINSVEQNDMLRKVTFDPEVFFNILLPPIIFHAGYSLKKRHFFRNLGSILAYAFLG
                     TAVSCFIIGNLMYGVVKLMKIMGQLSDKFYYTDCLFFGAIISATDPVTVLAIFNELHA
                     DVDLYALLFGESVLNDAVAIVLSSSIVAYQPAGLNTHAFDAAAFFKSVGIFLGIFSGS
                     FTMGAVTGVVTALVTKFTKLHCFPLLETALFFLMSWSTFLLAEACGFTGVVAVLFCGI
                     TQAHYTYNNLSVESRSRTKQLFEVLHFLAENFIFSYMGLALFTFQKHVFSPIFIIGAF
                     VAIFLGRAAHIYPLSFFLNLGRRHKIGWNFQHMMMFSGLRGAMAFALAIRDTASYARQ
                     MMFTTTLLIVFFTVWIIGGGTTPMLSWLNIRVGVEEPSEEDQNEHHWQYFRVGVDPDQ
                     DPPPNNDSFQVLQGDGPDSARGNRTKQESAWIFRLWYSFDHNYLKPILTHSGPPLTTT
                     LPAWCGLLARCLTSPQVYDNQEPLREEDSDFILTEGDLTLTYGDSTVTANGSSSSHTA
                     STSLEGSRRTKSSSEEVLERDLGMGDQKVSSRGTRLVFPLEDNA"
     exon            415..614
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     exon            615..692
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     exon            693..769
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     exon            770..882
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     exon            883..988
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1130
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     exon            1131..1236
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     exon            1237..1325
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..1439
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1551
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..1705
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     exon            1706..1765
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     exon            1766..1829
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     exon            1830..1912
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     exon            1913..2018
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..9971
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7184..7189
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /note="hexamer: ATTAAA"
     polyA_site      7207
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /note="major polyA site"
     regulatory      9730..9735
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /note="hexamer: AATAAA"
     polyA_site      9756
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /note="major polyA site"
     regulatory      9951..9956
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /note="hexamer: AATAAA"
     polyA_site      9971
                     /gene="SLC9A7"
                     /gene_synonym="MRX108; NHE-7; NHE7; SLC9A6"
                     /note="major polyA site"
ORIGIN      
        1 actccggcag gtccgcggtg ctgccagaac ccgccgacgg cgcgggctgc tggtccccgc
       61 tcggaggagg cgcggggggc cccgggacca tggagcctgg tgacgcggcg cgccctggct
      121 cgggtcgggc taccggggcg ccgccgccgc ggctgctgct gctgccgctg ctgctgggtt
      181 gggggctgcg agtcgcggcc gcggcctcgg cctcctcctc tggggcggcg gcggaggaca
      241 gcagcgccat ggaggagctc gctactgaga aggaggcgga ggagagccac cggcaagaca
      301 gcgtgagcct gctcaccttc atcctgctgc tcacgctcac catcctcacc atctggctct
      361 tcaagcaccg ccgggtgcgc tttctgcacg agaccgggct ggccatgatc tatgggctca
      421 tcgttggggt gatcctgagg tatggtaccc ctgctaccag tggccgtgac aaatcactca
      481 gctgcactca ggaagacagg gccttcagta ccttattagt gaatgtcagc ggaaagttct
      541 tcgaatacac tctgaaagga gaaatcagtc ctggcaagat caacagcgta gagcagaatg
      601 atatgctacg gaaggtaaca ttcgatccag aagtattttt caacattctt ctgcctccaa
      661 ttatttttca tgctggatac agcttaaaga agagacactt tttcagaaat cttggatcta
      721 tactggccta tgccttcttg gggactgctg tttcatgctt cattattgga aatctcatgt
      781 atggtgtggt gaagctcatg aagattatgg gacagctctc agataaattt tactacacag
      841 attgtctctt ttttggagca atcatctctg ccactgaccc agtgactgtg ctggcgatat
      901 ttaatgaatt gcatgcagac gtggatcttt acgcacttct ttttggagag agcgtcctaa
      961 atgatgctgt tgccattgta ctgtcctcgt ctattgttgc ctaccagcca gcgggactga
     1021 acactcacgc ctttgatgct gctgcctttt ttaagtcagt tggcattttt ctaggtatat
     1081 ttagtggctc ttttaccatg ggagctgtga ctggtgttgt gactgctcta gtgactaagt
     1141 ttaccaaact gcactgcttc cccctgctgg agacggcgct gttcttcctc atgtcctgga
     1201 gcacgtttct cttggcagaa gcctgcggat ttacaggtgt tgtagctgtc cttttctgtg
     1261 gaatcacaca agctcattac acctacaaca atctgtcggt ggaatcaaga agtcgaacca
     1321 agcagctctt tgaggtgtta catttcctgg cagagaactt catcttctcc tacatgggcc
     1381 tggcactgtt taccttccag aagcacgttt tcagccccat tttcatcatc ggagcttttg
     1441 ttgccatctt cctgggcaga gccgcgcaca tctacccgct ctccttcttc ctcaacttgg
     1501 gcagaaggca taagattggc tggaattttc aacacatgat gatgttttca ggcctcaggg
     1561 gagcaatggc atttgcgttg gccatccgtg acacggcatc ctatgctcgc cagatgatgt
     1621 tcacgaccac ccttctcatt gtgttcttca ctgtctggat cattggagga ggcacgacac
     1681 ccatgttgtc atggcttaac atcagagttg gcgtcgagga gccctccgaa gaggaccaga
     1741 atgaacacca ctggcagtac ttcagagttg gtgttgaccc cgatcaagac ccaccaccca
     1801 acaacgacag ctttcaagtc ttacaagggg acggcccaga ttctgccaga ggaaaccgga
     1861 caaaacagga gagcgcatgg atattcaggc tgtggtacag ctttgatcac aattacctga
     1921 agcccatcct cacacacagt ggtcccccac taaccaccac gctccccgcc tggtgtggct
     1981 tactagctcg atgtctgacc agtccccagg tgtacgataa ccaagagcca ctgagagagg
     2041 aagactctga tttcatcctg accgaaggcg acctgacatt gacctacggg gacagcacag
     2101 tgactgcaaa tggctcctca agttcgcaca ccgcctccac gagtctggag ggcagccgga
     2161 gaacgaagag cagctcggag gaagtgctgg agcgagacct gggaatggga gaccagaagg
     2221 tttcgagccg gggcacccgc ctagtgtttc ccctggaaga taatgcttga ctttcccccc
     2281 aagccctggc gcgatggggt aggctcccga tggggtgagg acagctgcaa gccctagtgt
     2341 tgttggaggt ggggcagtga ctagattgaa ctaactcttc tattttattg gggtctgaag
     2401 ctattgtaac acttaaaatt taactcacaa tgcagatggt gaggcaaaag tgtctctaaa
     2461 ttcagacaaa cgtagaccta ttcccacttt tttcacatag tagtgcgctg tttcagagtt
     2521 aaacaaacaa aaaaaaatag catgctttaa tggtctccta attcattcac ctgcagtatc
     2581 tgaacaaggc gaggcaggtg ctgggtgggg ggattcctcc catgagaggc tgcatctcag
     2641 tacacagcag tgcaagtcaa gctgaccata gaattatcaa gttaaagaga aggaaggcaa
     2701 ttgagatcgg aactccaagg attatgggaa acttggtgtt aaatgggaca gaaaacatga
     2761 aaaagcaatt tggaggctct ggcaacgaag tctgcttgac tgcaacctca tatcaggatt
     2821 cctgacttta tgctgcctgt gttttttcta gactgaagat ttgaaaatac atccacgaac
     2881 atttcaacat ggaacgaaga attatagttc cttctctggc tatatccata aagaagtagt
     2941 tatgaaatgt ttaaaaccaa aggcaaatag tgttatactc ttattttttg attaatctga
     3001 ggaaaggagg tatttagaaa ctgatgatgg tatcactgca aaaagcattc aacttttttt
     3061 ttttgttgag atggtgtttc actctgtcac ccaggctgga gtgcagtggc gtggtcttgg
     3121 ctcactgcaa cctctgcctc acgggttcaa gtgattctcc tgcctcagcc tcctgattag
     3181 ctgggattac aggtgcccac caccatgccc agctaatttt ttgtattttt ggtagagatg
     3241 gggtttcacc atgttgccca ggctggtgtc aaactcctga cctcgtgatc cacctccctt
     3301 ggcctcccaa agtgctggga ttacaggtgt gagccactgc acctggccag cattcaactt
     3361 ttgagggcta ccatatgagc tgacagccta gggaaataat cactaggact gaagtatcta
     3421 ctctgggttt agaaataaca ccaaattttg taggatgcta ttatctggga aagggaaggc
     3481 agcaagaaac ctacaaggca ccaggctaga attcagaggg aaagaaatct caatgcaaaa
     3541 aaattactgc aaagaaatac aagatagtag tctcaagact aggtagaatt ccaaactttg
     3601 agccgacgaa tgcatgagat ttcagtggcc acctgaggaa tcagagggca aacaatggaa
     3661 cattaagtat tttcagccca ctagaaagct ctgtcttcta taagcttaag agtccgagtg
     3721 tctttacacc actgagtgcc atgcagagaa gggtcatttt ccttgtatct ggggaggctg
     3781 ctgcaccagc ttaacatgtg ggtgtgattt gaaagttagg ttttcagttt ggattctttc
     3841 tggatgagct gttctgtctg cccacacctg tagtgctgaa atacagaaag acctctcggg
     3901 aaaagtttga gtttctctta atgtctctgt gcttcagtgc tagcttattt ttggcaaccc
     3961 aatttctaaa aaatgctttc aataacgtgg gcttttcttt ttttctttct ttcctttttt
     4021 tttttttttt tgagatggag tctcgctgtg tcacccaggc tggagtgcag tggcgcgatc
     4081 tcggcttact gcaacctccg cctcccgggt tcaagcgatt ctcttgcctc cgcctcctga
     4141 gtagctggga ttacaggcac gcgccaccac gcctggctaa ttttttgtat tttttagtag
     4201 agacggggtt tcaccatact ggccagtttt tcttatgatg gaagacaaaa tattgtgtcc
     4261 cagtctagtc cacccgtagt gaaatcagtc atttaatctt ctcaattcgc atgtttaatg
     4321 cttaattttt aaataagaca ttgcttcaca actttgtgtt tgaattgata atttgtgcaa
     4381 atcaggaaaa tacgtattta attaggttga gccatatgag tttgctgata ttcgaccatt
     4441 ttgtaaaaac aggagtggca atttcatatg gttcaataaa ataaaattga gaccgggcac
     4501 agtggcttat gcctgtaatc ccaacacttt gggaggctga ggcaggagga tcacttgagc
     4561 tcaggagttc aagaccagac tgggcaacat ggcaaaaccc tgtctctaca gaaatacaaa
     4621 aatgagccag gcatgatggc acatgcctgt agttctagct acttgggagg ctgaggtggg
     4681 aggattgctt gagcccaaga ggcagaggtt gcgatgagct gagatcatgc cactgcaatc
     4741 cagtctgggc aacagaatga gaccctgcct caaaaaaaaa aaaaaaatac agttgaatga
     4801 tttttattct cctctttcct ttttatctac aataaagtcc attttgattt tatggtcatc
     4861 catgggcttt ggaagtttga aaaatgctaa acaacctatc caaacccatg agccctcagg
     4921 gtcaactagg ctagtgaagg acttaagaat aagacctaca ccccacagcc agagaaagag
     4981 ctttcatagc ccctggaagg gaagttctta tatcctgggg tgaggttgca gacagcagct
     5041 accaggcctg gcagaggcct gtattatatg tgtgctggag tccagaacca gcacaggatt
     5101 ttttatttag cagagttatc ttagtaatga ccagtcatgc catataaatc taaaaggcaa
     5161 gacttgtttt gttttcattt ttgtttttgt tttgcagaga tggagagata ggctgcttgg
     5221 gtctttggtt gggtctttat aaagctttgc ttatcttata ttgcttactt aattttggag
     5281 ggaaaaaact aacagacaca ccctggtgat ggttctttgt tcacctcaag gctttgttgt
     5341 tgtgttgttg ttgtttgttt tttgttttgt tttgttttgt ttttagatgg agtctcgctc
     5401 tatcccccag gctggagtgc agtggcatga cctcggctca ctgcaatctc cacctcctgg
     5461 gttcaagcga ttctcctacc tcagcctccc gagtagctgg gattacaggc acatgccacc
     5521 acacccagct aattttttta tatttttagt agagacagag tttcaccatg ttgggcagga
     5581 tggtctcgat ctgctgacct cgtgatccac ccgcctctgc ctcccaaagt gctgggatta
     5641 caggcgtgag ccaccgcccc tggccattat tttgagtttt ttttagaaag cttttacaag
     5701 attacaagaa atgactctct tagggaagaa aaataaccac ccagtttccc ttcctagtct
     5761 tagttcaatt catagagtgt tgtgtttatc cccaaagggt cttgtagtct tcactgcaaa
     5821 catttgattc tgatctactc acatacaagg aaatgtagat tgccaagacc aaaactagag
     5881 gggtgggctt cccatcaggg ttttcctgtg tggagatgac aggggaaaca gggaggaaat
     5941 cctaattatg gcttgtcctc ttccctgaat tgctgtctac gacagtgtag ttccaatgta
     6001 aacaaggtgg gttggggatg gtttgtatag ggcaggaggc ccacccagaa gtcctaccca
     6061 gggaagagag ggcccagaat ctagttgcag catttgccca gtaaccctac cccggccttt
     6121 ctttgtgtgt taggaagctg gaaatgcagg ctggtctccc tttcttgcct ttgtcactaa
     6181 ccccgagctg tcagccattt ggaatcagaa ctgctttcca gtgttctaag tccttatttg
     6241 attacaaata tttggtgaat caaagtaagg cactttggtc ctaatttttg acctttggtc
     6301 ctaatttttg tttgtaagat gtggagaata accctgacct taccaggggt cctgggaatg
     6361 tgaatccatt cgtcaagtgg ttccacatct agaagaattt tgcctatgcc atgtcaggca
     6421 tcattcaaca caaggccaac tatctgctgc cagtctggca taagatcaga ttgaaactgc
     6481 taagtaatgg tacatccaaa taaacaaagt tcttccaaga gagtaaaccc aagtccatct
     6541 tatacacgta tttgttctgg gttggtgggg acgacagttc agccgagtcc gatgttctag
     6601 agcagaggtt ctcaaccctg gctgtgaatt aggatcatct ggccagtttt tgtttttaaa
     6661 tcagtgcaag ggccctcccc tcagagattc agattcagta gcttcaggtg ggacctggaa
     6721 tccatgcttt gtttggaacc tcaggagtgt tcttgatgtg cagccaggat tgacaagagc
     6781 ctttagtcta gagctcgcag cagacctcta gactatttgg aatctgattt taaagaatgg
     6841 cccagcagat gaaggtgttt ttttaaattg ttagtcatgt gaaacgaact gagtattgat
     6901 cagtaataca gacagggcag ctgtgagaga gtttgatgct aatctttgca gaactcaaga
     6961 acatactcca taaagtacag ctgaaaagat cacgaaaacc ttggagcctt cgaggaaaga
     7021 gaagggtgac ttggggtatg tatggggaga actaaaaacc tgtcatcttg gttcccactt
     7081 gtggggaata aagggaaggt attgtcttaa ctgagctgct aactaatcaa gcatgaaggt
     7141 ttctgggaag ctgttttgtt tttttttttt aatttcacct ggaattaaat taatagtccc
     7201 ctctggagtc agattttctt ttccttcacc tatcctcatg cctgagtcca cactggattc
     7261 ctcagaataa gggtgggccc atttactgct ggagcaaagt agaaggttgt ggtcctttgc
     7321 tatcacatgg tgatggtggt ggtttccgta gcaggtaatt aaggagatgc acacttttgt
     7381 atagccctga cagagagaag atgaaagctg ctattccttt tagtacactt tttgagaact
     7441 accagaaacc tgaactttct ctcctaggga agaaaatttg gaatttaaat aaggccacat
     7501 ttcttcctag gacacaatca caggaagggc atatggatcc gagcatccag ggtggttact
     7561 ggggtgatca gacaaataag cattgatgcc tggaaggcag aaatcacact gcgacatcaa
     7621 atgtttcatc aagcaaacag caaggatctc ttcaggctgt gttctctaga cccgaaaatg
     7681 tcctctgtgg tttggctgac aaatggaatc tctttagaaa acatgcaaaa gggtatctca
     7741 gctatccatg aacgattcgg tctaggacaa agaactaaca gttccttcca aaatagaggt
     7801 ctgttggtct gtttgacgtc atcccatcat cctatctctc cctgtccttg tctgcagtag
     7861 aagttaatgt aatcatacca gatgtttaat gtgaacaagt aatttccaaa tgtagaaaca
     7921 ccactgatgc acttaggggg aaaaacagtg gcagggtgtt agagcatctc atcagaggtg
     7981 aaaatcaccc acttggaaac ctactgtgca cccgttgtga gttctaagag tgtatcagaa
     8041 agctgatttt accatctgtt gtgaaagatt atctgcaatg agggctgcta gaaagtcagc
     8101 ctgctgatct cacaagtata tgacttctta ttccacttca aaagtgggga acttgacaag
     8161 tttcttgtct gccttttgat gttgattcta tagattaatt agttcaggaa caagtctttc
     8221 atatacatta gactggtatc attgagttct gagctattct gttgttcttt aactgacact
     8281 ctggattgcc agtgtcggct gctacattct gatacagtaa gtcaaaggaa tctagtggcc
     8341 aagagttgta gtttagaaac aacccatctg aatactgctt ttaccacttt gatacctgaa
     8401 aagatcatcc ccctttctaa aacctgaaaa caacatatag agaaatactg tagaaaccat
     8461 tgctagtgtc ttaactagga aaagcaagta gactcatcaa caatcctttc aaaacaagac
     8521 ctagagataa acagtttttt ccagtgtcaa ttttcataga catttttaaa aaattaaaca
     8581 catcttgtca cccagtgcta gcaaaaattg taaagagcaa aaccaaagtt tttgatgtta
     8641 gtcttatttt tgtaagcagg gtatactata gtcgtggccc atatggaaca cacaggcagc
     8701 cggttttctg ctacctgagc cttggagaag atgagaagga agggctgcct gctgtgctgc
     8761 cctttgaaaa ggtccaaagc agacaggagc gcacacagca gaagtcacct tctccttctg
     8821 atgaccacct ttcaaaaggc ttaacagaat caccaagtgt cacagtcagc tcttggccag
     8881 tggaaaattg tctgggcctg tatatgttgg aggaactcag atagaaaagg acttgtaaac
     8941 agcatggaaa cctcaacatc tgctgtctca agcacttaaa aaaggctaca attcaggtgt
     9001 tactggatga cttaactgca tggacattta ggggctgact tgtgttgtga ttattccaat
     9061 tgtgggcttt ggagaagcag agggatgatt ttcagttgtg tgggtgttaa gtctatggat
     9121 tgggaattta ccactgaaat tgatcgttca aagtgtgact tcatagtagg gccagctcct
     9181 cctaagttgt ccttttgttt ttcacataaa atacatctgg tactttttac ttggaaagtt
     9241 gtactatgtc catgaaaatg gttaaatgag aaggccataa agattcattt aatagctgaa
     9301 aagcaacttt taaaatagaa taggacgaat ttaacatgga atgatgtgac cttctaggga
     9361 actttttata gagagcttac atagtgtaat tggggagaat tttgtcttct atttccattc
     9421 aacagtagca aactggtggt tttataaaat taacattata gtatgttata taaataaacg
     9481 ttatataaaa tgacattaaa gtataactgt gaaaaattaa agacttggcg ttatccacaa
     9541 cctttatgtg caataaggaa aactgcagta ggcccccaaa gtgccgtctt tccctagcat
     9601 tttactcaat gtttgccttt gtgtatgttt agattgctat tgtaaagcta gccatgtgtg
     9661 ttcctgccct ttactgatat ttaacacatt ttctcaaagc ttttcttttt ttaaaaaaac
     9721 aaaactcaaa ataaaacaac taactggctg agctaatttt gttttgtatt ttaaagtaaa
     9781 gtatcatctg ttggtagaag gctgtagtgt gatactattc ttagtctgtt tccctcactg
     9841 agaggttctt ttctgggtgt aagctattcc tctccagctt tctttaggcc tggggttggc
     9901 aaactacagc tggctcctga accaagtcca gcccactgcc tttttttgta aataaagttt
     9961 tattggcatg a
//
