LOCUS       NM_004594               3708 bp    mRNA    linear   PRI 03-APR-2024
DEFINITION  Homo sapiens solute carrier family 9 member A5 (SLC9A5), transcript
            variant 1, mRNA.
ACCESSION   NM_004594
VERSION     NM_004594.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Kurata,T., Rajendran,V., Fan,S., Ohta,T., Numata,M. and Fushida,S.
  TITLE     NHE5 regulates growth factor signaling, integrin trafficking, and
            degradation in glioma cells
  JOURNAL   Clin Exp Metastasis 36 (6), 527-538 (2019)
   PUBMED   31595389
  REMARK    GeneRIF: High NHE5 expression is associated with glioma.
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Jinadasa,T., Szabo,E.Z., Numat,M. and Orlowski,J.
  TITLE     Activation of AMP-activated protein kinase regulates hippocampal
            neuronal pH by recruiting Na(+)/H(+) exchanger NHE5 to the cell
            surface
  JOURNAL   J Biol Chem 289 (30), 20879-20897 (2014)
   PUBMED   24936055
  REMARK    GeneRIF: These data reveal a unique role for AMPK and NHE5 in
            regulating the pH homeostasis of hippocampal neurons during
            metabolic stress.
REFERENCE   3  (bases 1 to 3708)
  AUTHORS   Diering,G.H., Numata,Y., Fan,S., Church,J. and Numata,M.
  TITLE     Endosomal acidification by Na+/H+ exchanger NHE5 regulates TrkA
            cell-surface targeting and NGF-induced PI3K signaling
  JOURNAL   Mol Biol Cell 24 (21), 3435-3448 (2013)
   PUBMED   24006492
REFERENCE   4  (bases 1 to 3708)
  AUTHORS   Diering,G.H., Mills,F., Bamji,S.X. and Numata,M.
  TITLE     Regulation of dendritic spine growth through activity-dependent
            recruitment of the brain-enriched Na/H exchanger NHE5
  JOURNAL   Mol Biol Cell 22 (13), 2246-2257 (2011)
   PUBMED   21551074
  REMARK    GeneRIF: overexpression of NHE5 inhibits spine growth in response
            to neuronal activity
REFERENCE   5  (bases 1 to 3708)
  AUTHORS   Lukashova,V., Szabo,E.Z., Jinadasa,T., Mokhov,A., Litchfield,D.W.
            and Orlowski,J.
  TITLE     CK2 phosphorylation of an acidic Ser/Thr di-isoleucine motif in the
            Na+/H+ exchanger NHE5 isoform promotes association with
            beta-arrestin2 and endocytosis
  JOURNAL   J Biol Chem 286 (13), 11456-11468 (2011)
   PUBMED   21296876
  REMARK    GeneRIF: discrete elements of an elaborate sorting signal in NHE5
            contribute to beta-arrestin2 binding and trafficking along the
            recycling endosomal pathway.
REFERENCE   6  (bases 1 to 3708)
  AUTHORS   Szaszi,K., Paulsen,A., Szabo,E.Z., Numata,M., Grinstein,S. and
            Orlowski,J.
  TITLE     Clathrin-mediated endocytosis and recycling of the neuron-specific
            Na+/H+ exchanger NHE5 isoform. Regulation by phosphatidylinositol
            3'-kinase and the actin cytoskeleton
  JOURNAL   J Biol Chem 277 (45), 42623-42632 (2002)
   PUBMED   12205089
  REMARK    GeneRIF: observations demonstrate that NHE5 is localized to the
            recycling endosomal pathway and is dynamically regulated by
            phosphatidylinositol 3'-kinase and by the state of F-actin assembly
REFERENCE   7  (bases 1 to 3708)
  AUTHORS   Szabo,E.Z., Numata,M., Shull,G.E. and Orlowski,J.
  TITLE     Kinetic and pharmacological properties of human brain Na(+)/H(+)
            exchanger isoform 5 stably expressed in Chinese hamster ovary cells
  JOURNAL   J Biol Chem 275 (9), 6302-6307 (2000)
   PUBMED   10692428
REFERENCE   8  (bases 1 to 3708)
  AUTHORS   Attaphitaya,S., Park,K. and Melvin,J.E.
  TITLE     Molecular cloning and functional expression of a rat Na+/H+
            exchanger (NHE5) highly expressed in brain
  JOURNAL   J Biol Chem 274 (7), 4383-4388 (1999)
   PUBMED   9933642
REFERENCE   9  (bases 1 to 3708)
  AUTHORS   Baird,N.R., Orlowski,J., Szabo,E.Z., Zaun,H.C., Schultheis,P.J.,
            Menon,A.G. and Shull,G.E.
  TITLE     Molecular cloning, genomic organization, and functional expression
            of Na+/H+ exchanger isoform 5 (NHE5) from human brain
  JOURNAL   J Biol Chem 274 (7), 4377-4382 (1999)
   PUBMED   9933641
REFERENCE   10 (bases 1 to 3708)
  AUTHORS   Klanke,C.A., Su,Y.R., Callen,D.F., Wang,Z., Meneton,P., Baird,N.,
            Kandasamy,R.A., Orlowski,J., Otterud,B.E., Leppert,M. et al.
  TITLE     Molecular cloning and physical and genetic mapping of a novel human
            Na+/H+ exchanger (NHE5/SLC9A5) to chromosome 16q22.1
  JOURNAL   Genomics 25 (3), 615-622 (1995)
   PUBMED   7759094
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB475730.1, AF111173.1 and AL137689.1.
            
            On Nov 22, 2018 this sequence version replaced NM_004594.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF111173.1, SRR1803613.246168.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2146236, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000299798.16/ ENSP00000299798.11
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-401               DB475730.1         32-432
            402-3114            AF111173.1         435-3147
            3115-3395           AL137689.1         1569-1849
            3396-3455           AF111173.1         3430-3489
            3456-3708           AL137689.1         1909-2161
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.1"
     gene            1..3708
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /note="solute carrier family 9 member A5"
                     /db_xref="GeneID:6553"
                     /db_xref="HGNC:HGNC:11078"
                     /db_xref="MIM:600477"
     exon            1..223
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..2727
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; sodium/hydrogen exchanger 5; NHE-5; solute
                     carrier family 9, subfamily A (NHE5, cation proton
                     antiporter 5), member 5; solute carrier family 9 member 5;
                     Na(+)/H(+) exchanger 5; solute carrier family 9
                     (sodium/hydrogen exchanger), member 5"
                     /codon_start=1
                     /product="sodium/hydrogen exchanger 5 isoform 1 precursor"
                     /protein_id="NP_004585.1"
                     /db_xref="CCDS:CCDS42178.1"
                     /db_xref="GeneID:6553"
                     /db_xref="HGNC:HGNC:11078"
                     /db_xref="MIM:600477"
                     /translation="MLRAALSLLALPLAGAAEEPTQKPESPGEPPPGLELFRWQWHEV
                     EAPYLVALWILVASLAKIVFHLSRKVTSLVPESCLLILLGLVLGGIVLAVAKKAEYQL
                     EPGTFFLFLLPPIVLDSGYFMPSRLFFDNLGAILTYAVVGTLWNAFTTGAALWGLQQA
                     GLVAPRVQAGLLDFLLFGSLISAVDPVAVLAVFEEVHVNETLFIIVFGESLLNDAVTV
                     VLYKVCNSFVEMGSANVQATDYLKGVASLFVVSLGGAAVGLVFAFLLALTTRFTKRVR
                     IIEPLLVFLLAYAAYLTAEMASLSAILAVTMCGLGCKKYVEANISHKSRTTVKYTMKT
                     LASCAETVIFMLLGISAVDSSKWAWDSGLVLGTLIFILFFRALGVVLQTWVLNQFRLV
                     PLDKIDQVVMSYGGLRGAVAFALVILLDRTKVPAKDYFVATTIVVVFFTVIVQGLTIK
                     PLVKWLKVKRSEHHKPTLNQELHEHTFDHILAAVEDVVGHHGYHYWRDRWEQFDKKYL
                     SQLLMRRSAYRIRDQIWDVYYRLNIRDAISFVDQGGHVLSSTGLTLPSMPSRNSVAET
                     SVTNLLRESGSGACLDLQVIDTVRSGRDREDAVMHHLLCGGLYKPRRRYKASCSRHFI
                     SEDAQERQDKEVFQQNMKRRLESFKSTKHNICFTKSKPRPRKTGRRKKDGVANAEATN
                     GKHRGLGFQDTAAVILTVESEEEEEESDSSETEKEDDEGIIFVARATSEVLQEGKVSG
                     SLEVCPSPRIIPPSPTCAEKELPWKSGQGDLAVYVSSETTKIVPVDMQTGWNQSISSL
                     ESLASPPCNQAPILTCLPPHPRGTEEPQVPLHLPSDPRSSFAFPPSLAKAGRSRSESS
                     ADLPQQQELQPLMGHKDHTHLSPGTATSHWCIQFNRGSRL"
     exon            224..526
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /inference="alignment:Splign:2.1.0"
     exon            527..690
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /inference="alignment:Splign:2.1.0"
     exon            691..769
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /inference="alignment:Splign:2.1.0"
     exon            770..947
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1168
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1371
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1461
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1532
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..1662
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /inference="alignment:Splign:2.1.0"
     exon            1663..1751
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1878
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..2049
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /inference="alignment:Splign:2.1.0"
     exon            2050..2116
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /inference="alignment:Splign:2.1.0"
     exon            2117..2254
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..3708
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3688..3693
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /note="hexamer: ATTAAA"
     polyA_site      3708
                     /gene="SLC9A5"
                     /gene_synonym="NHE5"
                     /note="major polyA site"
ORIGIN      
        1 gtgcggtgcc gggagggcgg ctgggcaggc ggcaggatgc tgcgcgccgc cctgtccctg
       61 ctcgcgctgc ccctggcggg ggcggccgaa gagcccaccc agaagccaga gtccccgggc
      121 gagcctcccc caggcttaga gctcttccgc tggcagtggc acgaggtgga ggcgccctac
      181 ctggtggccc tgtggatcct ggtggccagt ctggccaaaa tcgtgtttca cctgtctcgg
      241 aaagtaacat ctctggtccc tgagagctgc ctgctgattt tgctgggcct ggtgctaggg
      301 ggaattgttt tggctgtggc caagaaagct gagtaccagc tggagccagg caccttcttc
      361 ctcttcctgc tgcctcctat tgtgttggac tcaggctatt tcatgcctag caggctgttc
      421 tttgacaact tgggtgccat cctcacctat gccgtggtag gcacactctg gaatgccttc
      481 acaacaggcg ctgccctctg gggcttgcag caggctggac ttgtagcccc tagggtgcag
      541 gctggcttac tggacttcct gctgtttggg agcctcatct cggcggtgga ccccgtggcc
      601 gtgctagctg tctttgagga ggtgcacgtc aatgagactc tctttatcat cgtctttggc
      661 gagtccctgc tcaacgatgc tgtcaccgtg gtgctgtaca aggtctgcaa ctcctttgtg
      721 gagatgggct ctgccaatgt gcaggccact gactacctga agggagtcgc ctccctgttt
      781 gtggtcagtc tgggcggggc agccgtgggc ttagtctttg ccttcctcct ggccctgacc
      841 acacgcttca ccaagcgggt ccgcatcatc gagccgctgc tggtcttcct cctcgcctac
      901 gcagcctacc tcactgctga aatggcctcg ctctccgcca ttcttgcggt gaccatgtgt
      961 ggcctgggct gtaagaagta cgtggaggcc aacatctccc ataagtcacg cacaactgtc
     1021 aaatatacaa tgaagactct agccagctgt gctgagaccg tgatcttcat gctgcttggc
     1081 atctcagccg tggactcttc taagtgggcc tgggattctg ggctggtgct gggcaccctc
     1141 atcttcatcc tgttcttccg agccctcggc gtagtcctgc agacctgggt gctgaatcag
     1201 ttccggctag tccctctgga caagattgac caagtggtga tgtcctatgg gggcctgcgg
     1261 ggggctgtgg cctttgctct cgtcatccta ctggatagga ccaaggtccc tgccaaggac
     1321 tactttgtag ccaccactat tgtagtggtc ttcttcacag tcatcgtgca gggcttgacc
     1381 atcaagccac tggtcaaatg gctgaaggtg aagaggagtg agcatcacaa acccaccctg
     1441 aaccaggagc tgcatgaaca cacttttgac cacattctgg ctgcagtgga ggacgttgtg
     1501 gggcaccatg gctaccacta ctggagggac aggtgggagc agtttgacaa gaaatacctg
     1561 agtcagctgc tgatgcgacg atcagcctac cgcatccggg accagatctg ggatgtgtac
     1621 tacaggctta acatccggga tgccatcagc tttgtggacc agggaggcca cgtcttgtct
     1681 tccacaggtc tcactctgcc ttctatgccc agccgcaatt ctgtggcaga aacttctgtc
     1741 accaacctgc tgagggagag tggcagtgga gcgtgtctgg atctgcaggt gattgacaca
     1801 gtacgcagcg gccgggatcg tgaggatgct gtgatgcatc atctgctctg cggaggcctc
     1861 tacaagccgc gccgtaggta caaagccagc tgcagtcgcc acttcatctc agaggatgcg
     1921 caggagcggc aggacaagga ggtcttccag cagaacatga agcggcggct ggagtccttt
     1981 aagtccacca agcacaacat ctgcttcacc aagagcaagc cacgaccccg caagactggc
     2041 cgcaggaaga aggatggtgt ggcgaatgct gaggctacaa atgggaaaca tcgaggcctg
     2101 ggctttcagg acacagctgc tgtgatatta accgtggagt ctgaggagga ggaggaggag
     2161 agcgacagtt cagagacaga gaaggaggac gatgagggga tcatctttgt ggctcgtgcc
     2221 accagtgagg ttctccaaga gggcaaggtc tcaggaagcc ttgaggtgtg cccaagccca
     2281 cgaatcattc ccccctcccc aacctgtgca gaaaaggagc tcccctggaa gagtgggcag
     2341 ggggacctgg cagtgtacgt gtcctcggaa accaccaaga ttgtgcctgt ggacatgcag
     2401 acgggttgga accagagcat ctcatccctg gagagcctag cgtcccctcc ctgtaaccag
     2461 gccccaattc tgacctgcct gcctccccat ccacggggca ctgaagagcc ccaggtccct
     2521 ctccacctac cttctgatcc acgctctagc ttcgccttcc caccgagcct ggccaaggct
     2581 ggccgctctc gcagtgagag cagcgctgac ctcccccagc agcaggagct gcagcccctc
     2641 atgggccaca aggaccacac ccatctcagc ccaggcaccg ctacctccca ctggtgcatc
     2701 cagttcaaca gaggcagccg gctgtagctc aaggcctcgg ggaggagcag gaggtggaat
     2761 ccctgtggga agtgctccct gggtgatggg tagagccctc gaaacttgac atggggccag
     2821 aagggcctgg gttgaagtag taattgggct tccttggagc tagtcagagg ggtcacctaa
     2881 gctggtcctc acaggggcct ttctcaccac ctccctgctc ctaacccctg ccactttctg
     2941 tttcattaag gcctctactc tggctcagga cccagtccag gccttctacg ggctaggccc
     3001 agagacttgg gttgctggtc ccccttccct agtgggtttt cccggggact ctataggcag
     3061 ctgctcctgc ccgcaaagca agagcatcat tcctattctt cagtggatgc cagccttccc
     3121 tgccccaact ccctccccag cactgggtca gtggtgtcct ggcagtgagg ctccgtgagg
     3181 ggctggccct tagaggaact ggggtgggag gtggggcagg cctcaccctt gggctttgct
     3241 gccctgttgg gtcagctacc catagtccat ttttttaggg cagtgggaac ctctgcctcc
     3301 acttcctgct ttagcccctt ccctttgctg ccaggtattg gggtaatatt tcctcctttt
     3361 ccaagaccaa ggccaagagg ctgggccagg cttcagttca ggcctgttgc ttaactgggg
     3421 tcaccctggg atctgctgct ctgggtctaa gtctagacct ttctgatcct tgggtctggg
     3481 ttttttgagg aggggacata gtggcctctg ggctgccatg tcaccacctg aacattcccc
     3541 aaacagagaa ggaacccagc atctcagggc cactgctcca ttgctctggg ggctgggatg
     3601 cctggctaag caggggctga cagggtggca ggtgactttc tagggatcag cacctgccct
     3661 gtgttttgta ccttgaacct aagatatatt aaacatctct cagatgga
//
