LOCUS       NM_003048               5601 bp    mRNA    linear   PRI 23-JUL-2024
DEFINITION  Homo sapiens solute carrier family 9 member A2 (SLC9A2), mRNA.
ACCESSION   NM_003048
VERSION     NM_003048.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Liu,Y.M., Yang,T.C., Fang,X.C., Yang,L.J., Shi,L.W., Wang,H.Q.,
            Dou,T.T., Shu,L., Chen,T.L., Hu,J., Yu,X.M. and Li,X.F.
  TITLE     Identification and Validation of SLC9A2 as A Potential Tumor
            Suppressor in Colorectal Cancer: Integrating Bioinformatics
            Analysis with Experimental Confirmation
  JOURNAL   Curr Med Sci 44 (3), 529-544 (2024)
   PUBMED   38809379
  REMARK    GeneRIF: Identification and Validation of SLC9A2 as A Potential
            Tumor Suppressor in Colorectal Cancer: Integrating Bioinformatics
            Analysis with Experimental Confirmation.
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Zhang,X., Hou,J., Zhou,G., Wang,H. and Wu,Z.
  TITLE     zDHHC3-mediated S-palmitoylation of SLC9A2 regulates apoptosis in
            kidney clear cell carcinoma
  JOURNAL   J Cancer Res Clin Oncol 150 (4), 194 (2024)
   PUBMED   38619631
  REMARK    GeneRIF: zDHHC3-mediated S-palmitoylation of SLC9A2 regulates
            apoptosis in kidney clear cell carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5601)
  AUTHORS   Zhou,K., Amiri,M., Salari,A., Yu,Y., Xu,H., Seidler,U. and
            Nikolovska,K.
  TITLE     Functional characterization of the sodium/hydrogen exchanger 8 and
            its role in proliferation of colonic epithelial cells
  JOURNAL   Am J Physiol Cell Physiol 321 (3), C471-C488 (2021)
   PUBMED   34288721
  REMARK    GeneRIF: Functional characterization of the sodium/hydrogen
            exchanger 8 and its role in proliferation of colonic epithelial
            cells.
REFERENCE   4  (bases 1 to 5601)
  AUTHORS   Yu,Y., Seidler,A., Zhou,K., Yuan,Z., Yeruva,S., Amiri,M., Yun,C.C.,
            Nikolovska,K. and Seidler,U.
  TITLE     Expression, Localization and Functional Activity of the Major Na/H
            Exchange Isoforms Expressed in the Intestinal Cell Line Caco-2BBe
  JOURNAL   Cell Physiol Biochem 52 (5), 1017-1038 (2019)
   PUBMED   30977986
  REMARK    GeneRIF: Differentiated Caco-2BBe cells display particularly high
            mRNA expression levels of NHE2, which can be functionally
            identified in the apical membrane. Although at low intracellular
            pH, NHE2 transport rate was far lower than that of NHE1. NHE2
            activity was nevertheless essential for the maintenance of the
            steady-state pHi of these cells.
REFERENCE   5  (bases 1 to 5601)
  AUTHORS   Sarker,R., Cha,B., Kovbasnjuk,O., Cole,R., Gabelli,S., Tse,C.M. and
            Donowitz,M.
  TITLE     Phosphorylation of NHE3-S719 regulates NHE3 activity through the
            formation of multiple signaling complexes
  JOURNAL   Mol Biol Cell 28 (13), 1754-1767 (2017)
   PUBMED   28495796
  REMARK    GeneRIF: Studies show that phosphorylation of the NHE3 at a single
            amino acid in the distal part of the C-terminus affects multiple
            aspects of NHE3 complex formation and changes the NHE3 lipid raft
            distribution, which cause changes in specific aspects of basal as
            well as acutely stimulated and inhibited Na(+)/H(+) exchange
            activity.
REFERENCE   6  (bases 1 to 5601)
  AUTHORS   Malakooti,J., Dahdal,R.Y., Dudeja,P.K., Layden,T.J. and
            Ramaswamy,K.
  TITLE     The human Na(+)/H(+) exchanger NHE2 gene: genomic organization and
            promoter characterization
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 280 (4), G763-G773 (2001)
   PUBMED   11254504
REFERENCE   7  (bases 1 to 5601)
  AUTHORS   Ghishan,F.K., Knobel,S.M. and Summar,M.
  TITLE     Molecular cloning, sequencing, chromosomal localization, and tissue
            distribution of the human Na+/H+ exchanger (SLC9A2)
  JOURNAL   Genomics 30 (1), 25-30 (1995)
   PUBMED   8595899
REFERENCE   8  (bases 1 to 5601)
  AUTHORS   Collins,J.F., Honda,T., Knobel,S., Bulus,N.M., Conary,J., DuBois,R.
            and Ghishan,F.K.
  TITLE     Molecular cloning, sequencing, tissue distribution, and functional
            expression of a Na+/H+ exchanger (NHE-2)
  JOURNAL   Proc Natl Acad Sci U S A 90 (9), 3938-3942 (1993)
   PUBMED   7683411
REFERENCE   9  (bases 1 to 5601)
  AUTHORS   Kulanthaivel,P., Furesz,T.C., Moe,A.J., Smith,C.H., Mahesh,V.B.,
            Leibach,F.H. and Ganapathy,V.
  TITLE     Human placental syncytiotrophoblast expresses two pharmacologically
            distinguishable types of Na(+)-H+ exchangers, NHE-1 in the
            maternal-facing (brush border) membrane and NHE-2 in the
            fetal-facing (basal) membrane
  JOURNAL   Biochem J 284 (Pt 1) (Pt 1), 33-38 (1992)
   PUBMED   1318024
REFERENCE   10 (bases 1 to 5601)
  AUTHORS   Rauch,F.H., Schleicher,A. and Zilles,K.
  TITLE     Quantitative analysis of the retrograde reaction of motoneurons
            with an image analyser
  JOURNAL   Anat Embryol (Berl) 181 (4), 373-379 (1990)
   PUBMED   2346229
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF273748.1, BC136377.1,
            BC035787.1, AC007239.3 and AA843986.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_003048.5.
            
            Summary: This gene encodes a member of the sodium-hydrogen
            exchanger (NHE) protein family. These proteins are involved in
            sodium-ion transport by exchanging intracellular hydrogen ions to
            external sodium ions and help in the regulation of cell pH and
            volume. The encoded protein is localized to the apical membrane and
            is involved in apical absorption of sodium. [provided by RefSeq,
            Jun 2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.84593.1,
                                           SRR1803614.213018.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000233969.3/ ENSP00000233969.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-136               AF273748.1         1070-1205
            137-3242            BC136377.1         1-3106
            3243-4921           BC035787.1         3088-4766
            4922-5542           AC007239.3         119792-120412
            5543-5596           AA843986.1         5-58                c
            5597-5601           AC007239.3         120467-120471
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q12.1"
     gene            1..5601
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /note="solute carrier family 9 member A2"
                     /db_xref="GeneID:6549"
                     /db_xref="HGNC:HGNC:11072"
                     /db_xref="MIM:600530"
     exon            1..585
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /inference="alignment:Splign:2.1.0"
     CDS             297..2735
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /note="sodium/hydrogen exchanger 2; Na(+)/H(+) exchanger
                     2; solute carrier family 9 (sodium/hydrogen exchanger),
                     member 2; solute carrier family 9, subfamily A (NHE2,
                     cation proton antiporter 2), member 2"
                     /codon_start=1
                     /product="sodium/hydrogen exchanger 2 precursor"
                     /protein_id="NP_003039.2"
                     /db_xref="CCDS:CCDS2062.1"
                     /db_xref="GeneID:6549"
                     /db_xref="HGNC:HGNC:11072"
                     /db_xref="MIM:600530"
                     /translation="MEPLGNWRSLRAPLPPMLLLLLLQVAGPVGALAETLLNAPRAMG
                     TSSSPPSPASVVAPGTTLFEESRLPVFTLDYPHVQIPFEITLWILLASLAKIGFHLYH
                     KLPTIVPESCLLIMVGLLLGGIIFGVDEKSPPAMKTDVFFLYLLPPIVLDAGYFMPTR
                     PFFENIGTIFWYAVVGTLWNSIGIGVSLFGICQIEAFGLSDITLLQNLLFGSLISAVD
                     PVAVLAVFENIHVNEQLYILVFGESLLNDAVTVVLYNLFKSFCQMKTIETIDVFAGIA
                     NFFVVGIGGVLIGIFLGFIAAFTTRFTHNIRVIEPLFVFLYSYLSYITAEMFHLSGIM
                     AITACAMTMNKYVEENVSQKSYTTIKYFMKMLSSVSETLIFIFMGVSTVGKNHEWNWA
                     FVCFTLAFCLMWRALGVFVLTQVINRFRTIPLTFKDQFIIAYGGLRGAICFALVFLLP
                     AAVFPRKKLFITAAIVVIFFTVFILGITIRPLVEFLDVKRSNKKQQAVSEEIYCRLFD
                     HVKTGIEDVCGHWGHNFWRDKFKKFDDKYLRKLLIRENQPKSSIVSLYKKLEIKHAIE
                     MAETGMISTVPTFASLNDCREEKIRKVTSSETDEIRELLSRNLYQIRQRTLSYNRHSL
                     TADTSERQAKEILIRRRHSLRESIRKDSSLNREHRASTSTSRYLSLPKNTKLPEKLQK
                     RRTISIADGNSSDSDADAGTTVLNLQPRARRFLPEQFSKKSPQSYKMEWKNEVDVDSG
                     RDMPSTPPTPHSREKGTQTSGLLQQPLLSKDQSGSEREDSLTEGIPPKPPPRLVWRAS
                     EPGSRKARFGSEKP"
     exon            586..1049
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1300
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1518
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1721
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1811
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..1882
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1883..2044
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2045..2141
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2142..2273
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2274..2364
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2365..5601
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5390..5395
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /note="hexamer: CATAAA"
     polyA_site      5412
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
     regulatory      5537..5542
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /note="hexamer: ATTAAA"
     polyA_site      5560
                     /gene="SLC9A2"
                     /gene_synonym="NHE2"
                     /note="major polyA site"
ORIGIN      
        1 ggagagcagc gcaccggcat gggcaggcgg ccggcggcgg agggcggctg agggctgctg
       61 agggtacgcg cagcggcctc tcgtcgccct gcacgtgcct cgccaggcag tgcgcctgct
      121 cgcagcgagg acctagccct ctggttgcag agacccggtg ccgcagcagc ggcgggtggc
      181 tgtcgctgcc ctgccctgca cggggcaggg cggagcgggc tgagcagccc gggcgcgatg
      241 cgttgagcgc tcggagggcc aaccgccggt ccccttggcg gcaaccggcg gcacccatgg
      301 aaccactggg caactggagg agcctgcggg cgccactgcc tccgatgctg ttgctgctgc
      361 tcctgcaggt ggcggggccc gtgggcgccc tggcggagac cttgctgaac gcgccgaggg
      421 ccatgggcac cagttccagc ccgcctagcc ctgcgagcgt ggtggctccc ggaacgacgc
      481 tgttcgagga gagccggctg cctgtgttta cgctggatta cccccacgtg cagatcccct
      541 tcgagatcac cctttggatc ctgctggcct ccctggccaa gattggcttc catctgtatc
      601 acaagttgcc cacaatagtg cctgagagct gccttcttat aatggttgga cttctactag
      661 gtgggattat ttttggtgtt gatgagaagt ctccccctgc aatgaagact gatgtatttt
      721 tcttgtacct cctcccaccc atcgtgctgg atgccggcta tttcatgccc actcgcccat
      781 tctttgagaa cattggcacg attttctggt atgctgtggt agggacactt tggaattcca
      841 ttggcattgg ggtgtctttg tttggtatct gccagatcga agcattcggc ctcagcgaca
      901 tcactttgct ccagaacctg ctctttggca gcttaatctc agctgtcgat cctgtggctg
      961 tgcttgctgt ctttgagaac attcacgtca atgagcagct ctacatcctg gtctttggag
     1021 agtccctgct gaatgatgca gtaacagtgg tcctgtacaa cttgttcaag tcgttttgcc
     1081 agatgaaaac cattgagacc attgatgtgt ttgcaggaat cgccaacttc tttgttgtgg
     1141 gaatcggtgg ggtgctgatt ggcatcttct tgggctttat agcggcattt actactcgat
     1201 tcacccataa tatccgagtg atcgagccac tgtttgtttt cctgtacagt tatttgtcct
     1261 acatcacagc tgaaatgttt cacctctcag gcatcatggc aatcactgct tgtgcaatga
     1321 ctatgaataa gtacgtagaa gaaaatgtat ctcagaaatc ctacacgacc atcaagtact
     1381 tcatgaagat gctgagcagt gtcagcgaaa ccttgatctt catcttcatg ggtgtgtcta
     1441 ccgtgggcaa gaaccacgag tggaactggg ccttcgtctg cttcaccctg gccttctgcc
     1501 tcatgtggcg agccctgggt gtttttgtcc tgactcaggt cattaatagg ttccggacca
     1561 ttcccctgac ctttaaggac cagttcatca ttgcctatgg aggacttcga ggtgccatct
     1621 gttttgcgtt agtgtttctc cttcctgctg ctgtgtttcc tcggaaaaaa ttgtttatta
     1681 cggctgccat tgttgtcata ttctttactg tcttcattct gggaataact attcgaccac
     1741 tggtggagtt tcttgatgtc aagaggtcca ataagaaaca acaagctgtc agtgaagaaa
     1801 tctattgtcg gttgtttgat catgtgaaga ctggaattga agatgtttgt ggacattggg
     1861 gtcacaactt ttggagagac aagtttaaga agtttgatga taaatatctg cggaagcttt
     1921 tgattcggga aaaccaacca aagtcaagta ttgtatcttt atataaaaag cttgaaataa
     1981 aacatgccat tgagatggca gagactggga tgataagtac tgtccctaca tttgcatctc
     2041 taaatgattg tcgtgaagaa aaaataagga aggtcacgtc cagtgaaact gatgaaattc
     2101 gagaactctt atcaagaaat ctctatcaaa tccgtcagcg aactttatcc tacaacagac
     2161 acagtctgac agccgacaca agtgagagac aagccaagga gattctgatt cgccggcgac
     2221 acagtttgcg agaaagcatt aggaaggaca gcagcttgaa tcgagaacac agggcttcca
     2281 cttcaacctc ccgatattta tccttaccta aaaatacgaa gcttccagaa aagctacaaa
     2341 agaggaggac tatttctatt gcagatggca atagcagcga ctcagacgca gatgccggga
     2401 ccaccgtgct caatttgcag cccagagcca ggcgcttctt gccagaacag ttctccaaga
     2461 aatcccccca gtcctataaa atggaatgga agaatgaggt agatgttgat tctggccgag
     2521 atatgcccag caccccccca acaccccaca gcagagaaaa gggcacccag acgtcaggct
     2581 tactacagca gccccttctc tctaaagacc agtctggctc agagagggaa gacagtttga
     2641 ctgaaggcat cccgcccaag ccgccaccac ggctggtctg gagggcatcg gaacctggaa
     2701 gccggaaagc ccgatttggg agtgagaagc cttaagagaa gcagcgaaag cagatctgag
     2761 tgtctgaccc aggacagctg tggtttgtca ctctgaaacc tgatgcaaca gtggaatcca
     2821 tgtaaaactc tctgtgcatc taaatacttc tggagggcga cagattcatg ccacggataa
     2881 atgaggcaaa tccgaagaaa aggaaaatcg aataaaaaat agtcccacaa aatacctttt
     2941 gtgactaatg ggtagcaatc gtattatttg ctggcctgaa gagaaaaaat ggtaatgtgt
     3001 gcctttattg aacttgaatg acagaacttg aaatttttaa cacatccttc ttggtgaaga
     3061 ttttaatata ttacttatat gcttcaaatt ttatttatga aaaaatatgt atatctgtaa
     3121 tcagttgtta agtgaatggc actaaaagta tcgagaacag ctttctttcc caggggtgaa
     3181 ggatggcgct cgggggtgac tcctaagctc agcgtggaag cttttccctg tcctgacccg
     3241 atggagcagc cggtaagtga agagcacagc tggaagcaga gacaccttat gactcaaact
     3301 gggcaaacaa tcggaacgtc atgcagaagg aatggcctga ctcctgcagt gcagattgga
     3361 ggccccagaa ctcctatgaa ctactgagag tctactggtt tttaacttgt tcctctgtct
     3421 actgtgtgtt tgggggtgac atctaaattc catttcttct tcttgtttaa atggtgcaga
     3481 gagaaaggtt gcccagacat ccaccagctt actgtgttga cagatttggg agcagctttt
     3541 gatgaagttc cacccatgag ggtccatgtg gcaagtaggg ccacagggca gcacctggtt
     3601 acaacttgct ggtgggtttt atgttgtgca atactaggag gaggaggcag gtgatctatg
     3661 ctgatctatg atcacctgct tcagtagaat ttttctgtag agtgttgttt gaattttgag
     3721 cctccaggtt agaggctcca agcagacaaa gaagaaagtt tgtagacaca atattctaac
     3781 attgcacaag ttgatgagat atcaagtttt gaatattatt caggaagcac tttggagaat
     3841 gtgggagtcc tactgttttg cactagtctg taatttgtta cctctcccca ttcgataata
     3901 tagtaactca gaattttcat ataaaattga attctactca ttagagtact ttttaaaagt
     3961 acagcagaaa gaaaagaagt ggaacataag cattcaagat taatatttga ttctctatct
     4021 cttaactgta gattttattg gcctgttttt tttttctaaa taattcttta aaacttgact
     4081 ggaacatgcc tctgcatttc tgagtgtaga aattaaatga agccactcac agtcctttga
     4141 tttcccactg aagatacccc aaaggatgca agtgcctaca gtattatcag gaggagaaca
     4201 tgaaaatatt aagacaaaaa tcctcagcag ttgttctcca ccccttctcc acctccacca
     4261 gcaaggagaa gtcaatctac acttttttct catgttctaa agtcttagaa tactgctgga
     4321 ttgttgagca tgagacagag caaaggttag atacacaagg tacaagttca taggagcaca
     4381 attccttgat tcagggaaag tagcacagaa tgcaatttga aaactacagg cttaggactc
     4441 tgtgccaaaa atctctattt taaatgaaaa tatttatata aaaatacatt tattctgctt
     4501 aacacaaatt aaaactgaca taaaatttta tgataataac atgattcaag aaatgtgatg
     4561 tagatttttg agaatgccaa aaatcaggtt tcagaaaagc tacagtatga aatctgaaaa
     4621 gcaacagaaa ttgaaagtga ccttaaagaa taaatttcct aatttttccc agtttcctat
     4681 gtttataact attataaaga gtttaacttt ctggaactga aggagaaaac atgaaaatgt
     4741 ttatattgct tcttgttaag caggtagacc aaattccgct ttcagtaatt catccataca
     4801 gaatttggat gactatgtat aaaggaaaac ttaaatctta attattatca gtttaaattt
     4861 tttttgtttg ttgaatgaca aaggcaataa aaataaattt gactttagct ttttttctat
     4921 actttcctct ttttgtaatt cttaaattct agtcactagt cattatgaga gtattgacta
     4981 aatattttca caatctaaat attgtcacaa tctaaatttc tgtagtagag agagccgtag
     5041 ccctttgtaa aggctttcgt tcgtccaaac aacacttgat ccaaccaaag ttccaatgtg
     5101 actgtggatc tttgataggt ctttggtgtt ggttgtaaca aaatttgatt tgaactacct
     5161 ataaatgaaa agtcggggtt tattacttta tatgtaatgc tgagaggaga ctgttgggaa
     5221 cagttatgtc aatgagcaaa ctagaaattg gcttcaaagt ctaatacttt taaaagcatg
     5281 tgttttgtgt atatgctcac aaaatgtctg tgaagagatt tctttcagct tttgctcagc
     5341 tttatggtgg ggtgtactgt tttatctggg cggtgagggt attgatttac ataaaaactg
     5401 tagacaaaca cagtacgagt ctcagcacgt tttgcattta ttgtactgcc caaattgttt
     5461 ttatgttaaa agtcacgttt cataatctgc aatatttttg tcaaagtgca cactgtactt
     5521 tcttcttaaa aacgtgatta aagagatcac tttgtccgga tgaagcagct gttggcatta
     5581 tccgctgagg cttgaacacc a
//
