LOCUS       NM_014331               9645 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens solute carrier family 7 member 11 (SLC7A11), mRNA.
ACCESSION   NM_014331
VERSION     NM_014331.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Guo,Y., Wang,X., Du,Y., Zhao,Y., Gao,L., Hao,Y., Lv,D., Feng,X.,
            Zhai,Y., Zou,B., Han,J., Xu,E., Yang,Y., Yang,B., Xi,Y. and
            Zhang,L.
  TITLE     The splicing factor SF3B1 confers ferroptosis resistance and
            promotes lung adenocarcinoma progression via upregulation of
            SLC7A11
  JOURNAL   Cancer Gene Ther 31 (10), 1498-1510 (2024)
   PUBMED   39127833
  REMARK    GeneRIF: The splicing factor SF3B1 confers ferroptosis resistance
            and promotes lung adenocarcinoma progression via upregulation of
            SLC7A11.
REFERENCE   2  (bases 1 to 9645)
  AUTHORS   Zhang,X., Wang,C., Yan,Z. and Kong,X.
  TITLE     Expression and significance of cystine transporter SLC7A11/xCT in
            early colorectal cancer specimens from endoscopic submucosal
            dissection
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 70 (8), 207-212 (2024)
   PUBMED   39262240
  REMARK    GeneRIF: Expression and significance of cystine transporter
            SLC7A11/xCT in early colorectal cancer specimens from endoscopic
            submucosal dissection.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9645)
  AUTHORS   Namani,A., Veeraiyan,D. and Patra,T.
  TITLE     A comparative analysis indicates SLC7A11 expression regulate the
            prognostic value of KEAP1-NFE2L2-CUL3 mutations in human uterine
            corpus endometrial carcinoma
  JOURNAL   Free Radic Biol Med 222, 223-228 (2024)
   PUBMED   38876457
  REMARK    GeneRIF: A comparative analysis indicates SLC7A11 expression
            regulate the prognostic value of KEAP1-NFE2L2-CUL3 mutations in
            human uterine corpus endometrial carcinoma.
REFERENCE   4  (bases 1 to 9645)
  AUTHORS   Wang,J., Zhang,Z., Zhuang,J., Kang,D. and Song,W.
  TITLE     CircCOL5A1 is involved in proliferation, invasion, and inhibition
            of ferroptosis of colorectal cancer cells via miR-1287-5p/SLC7A11
  JOURNAL   J Biochem Mol Toxicol 38 (8), e23772 (2024)
   PUBMED   39030862
  REMARK    GeneRIF: CircCOL5A1 is involved in proliferation, invasion, and
            inhibition of ferroptosis of colorectal cancer cells via
            miR-1287-5p/SLC7A11.
REFERENCE   5  (bases 1 to 9645)
  AUTHORS   Zhou,Q., Yu,H., Chen,Y., Ren,J., Lu,Y. and Sun,Y.
  TITLE     The CRL3KCTD10 ubiquitin ligase-USP18 axis coordinately regulates
            cystine uptake and ferroptosis by modulating SLC7A11
  JOURNAL   Proc Natl Acad Sci U S A 121 (28), e2320655121 (2024)
   PUBMED   38959043
  REMARK    GeneRIF: The CRL3[KCTD10] ubiquitin ligase-USP18 axis coordinately
            regulates cystine uptake and ferroptosis by modulating SLC7A11.
REFERENCE   6  (bases 1 to 9645)
  AUTHORS   Kim,J.Y., Kanai,Y., Chairoungdua,A., Cha,S.H., Matsuo,H., Kim,D.K.,
            Inatomi,J., Sawa,H., Ida,Y. and Endou,H.
  TITLE     Human cystine/glutamate transporter: cDNA cloning and upregulation
            by oxidative stress in glioma cells
  JOURNAL   Biochim Biophys Acta 1512 (2), 335-344 (2001)
   PUBMED   11406111
REFERENCE   7  (bases 1 to 9645)
  AUTHORS   Shih,A.Y. and Murphy,T.H.
  TITLE     xCt cystine transporter expression in HEK293 cells: pharmacology
            and localization
  JOURNAL   Biochem Biophys Res Commun 282 (5), 1132-1137 (2001)
   PUBMED   11302733
REFERENCE   8  (bases 1 to 9645)
  AUTHORS   Bridges,C.C., Kekuda,R., Wang,H., Prasad,P.D., Mehta,P., Huang,W.,
            Smith,S.B. and Ganapathy,V.
  TITLE     Structure, function, and regulation of human cystine/glutamate
            transporter in retinal pigment epithelial cells
  JOURNAL   Invest Ophthalmol Vis Sci 42 (1), 47-54 (2001)
   PUBMED   11133847
REFERENCE   9  (bases 1 to 9645)
  AUTHORS   Sato,H., Tamba,M., Kuriyama-Matsumura,K., Okuno,S. and Bannai,S.
  TITLE     Molecular cloning and expression of human xCT, the light chain of
            amino acid transport system xc-
  JOURNAL   Antioxid Redox Signal 2 (4), 665-671 (2000)
   PUBMED   11213471
REFERENCE   10 (bases 1 to 9645)
  AUTHORS   Sato,H., Tamba,M., Ishii,T. and Bannai,S.
  TITLE     Cloning and expression of a plasma membrane cystine/glutamate
            exchange transporter composed of two distinct proteins
  JOURNAL   J Biol Chem 274 (17), 11455-11458 (1999)
   PUBMED   10206947
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC093903.3, AC116610.4 and AC110804.3.
            
            On Nov 23, 2018 this sequence version replaced NM_014331.3.
            
            Summary: This gene encodes a member of a heteromeric,
            sodium-independent, anionic amino acid transport system that is
            highly specific for cysteine and glutamate. In this system,
            designated Xc(-), the anionic form of cysteine is transported in
            exchange for glutamate. This protein has been identified as the
            predominant mediator of Kaposi sarcoma-associated herpesvirus
            fusion and entry permissiveness into cells. Also, increased
            expression of this gene in primary gliomas (compared to normal
            brain tissue) was associated with increased glutamate secretion via
            the XCT channels, resulting in neuronal cell death. [provided by
            RefSeq, Sep 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853566.21540.1,
                                           SRR11853564.21433.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000280612.9/ ENSP00000280612.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-557               AC093903.3         17329-17885         c
            558-684             AC093903.3         11861-11987         c
            685-800             AC093903.3         7803-7918           c
            801-926             AC116610.4         739-864             c
            927-1026            AC110804.3         59002-59101         c
            1027-1071           AC110804.3         54321-54365         c
            1072-1195           AC110804.3         24857-24980         c
            1196-1299           AC110804.3         22938-23041         c
            1300-1396           AC110804.3         22033-22129         c
            1397-1546           AC110804.3         20377-20526         c
            1547-1724           AC110804.3         18953-19130         c
            1725-9645           AC110804.3         3833-11753          c
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q28.3"
     gene            1..9645
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /note="solute carrier family 7 member 11"
                     /db_xref="GeneID:23657"
                     /db_xref="HGNC:HGNC:11059"
                     /db_xref="MIM:607933"
     exon            1..557
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /inference="alignment:Splign:2.1.0"
     CDS             281..1786
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /note="solute carrier family 7, (cationic amino acid
                     transporter, y+ system) member 11; amino acid transport
                     system xc-; calcium channel blocker resistance protein
                     CCBR1; solute carrier family 7 (anionic amino acid
                     transporter light chain, xc- system), member 11"
                     /codon_start=1
                     /product="cystine/glutamate transporter"
                     /protein_id="NP_055146.1"
                     /db_xref="CCDS:CCDS3742.1"
                     /db_xref="GeneID:23657"
                     /db_xref="HGNC:HGNC:11059"
                     /db_xref="MIM:607933"
                     /translation="MVRKPVVSTISKGGYLQGNVNGRLPSLGNKEPPGQEKVQLKRKV
                     TLLRGVSIIIGTIIGAGIFISPKGVLQNTGSVGMSLTIWTVCGVLSLFGALSYAELGT
                     TIKKSGGHYTYILEVFGPLPAFVRVWVELLIIRPAATAVISLAFGRYILEPFFIQCEI
                     PELAIKLITAVGITVVMVLNSMSVSWSARIQIFLTFCKLTAILIIIVPGVMQLIKGQT
                     QNFKDAFSGRDSSITRLPLAFYYGMYAYAGWFYLNFVTEEVENPEKTIPLAICISMAI
                     VTIGYVLTNVAYFTTINAEELLLSNAVAVTFSERLLGNFSLAVPIFVALSCFGSMNGG
                     VFAVSRLFYVASREGHLPEILSMIHVRKHTPLPAVIVLHPLTMIMLFSGDLDSLLNFL
                     SFARWLFIGLAVAGLIYLRYKCPDMHRPFKVPLFIPALFSFTCLFMVALSLYSDPFST
                     GIGFVITLTGVPAYYLFIIWDKKPRWFRIMSEKITRTLQIILEVVPEEDKL"
     exon            558..684
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /inference="alignment:Splign:2.1.0"
     exon            685..800
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /inference="alignment:Splign:2.1.0"
     exon            801..926
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1026
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1071
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1195
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1299
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1396
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1546
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..1724
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /inference="alignment:Splign:2.1.0"
     exon            1725..9645
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1871..1876
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /note="hexamer: ATTACA"
     polyA_site      1895
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
     regulatory      9621..9626
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /note="hexamer: AATAAA"
     polyA_site      9645
                     /gene="SLC7A11"
                     /gene_synonym="CCBR1; xCT"
                     /note="major polyA site"
ORIGIN      
        1 ggtttgtaat gatagggcgg cagcagcagc agcagcagca gtggtggaac gaggaggtgg
       61 agaattgaga gcacgatgca tacacaggtg tttctgagta gtaattagat cgctgtgaag
      121 gaaaaagcac acctttgagt tttcacctgt gaacactata gcgctgagag agacagtctg
      181 aaagcagagg aagacatcga tcagtaacac caagagacac caaagttgaa agttttgttt
      241 tctttccctc tgttttattt ttcccccgtg tgtccctact atggtcagaa agcctgttgt
      301 gtccaccatc tccaaaggag gttacctgca gggaaatgtt aacgggaggc tgccttccct
      361 gggcaacaag gagccacctg ggcaggagaa agtgcagctg aagaggaaag tcactttact
      421 gaggggagtc tccattatca ttggcaccat cattggagca ggaatcttca tctctcctaa
      481 gggcgtgctc cagaacacgg gcagcgtggg catgtctctg accatctgga cggtgtgtgg
      541 ggtcctgtca ctatttggag ctttgtctta tgctgaattg ggaacaacta taaagaaatc
      601 tggaggtcat tacacatata ttttggaagt ctttggtcca ttaccagctt ttgtacgagt
      661 ctgggtggaa ctcctcataa tacgccctgc agctactgct gtgatatccc tggcatttgg
      721 acgctacatt ctggaaccat tttttattca atgtgaaatc cctgaacttg cgatcaagct
      781 cattacagct gtgggcataa ctgtagtgat ggtcctaaat agcatgagtg tcagctggag
      841 cgcccggatc cagattttct taaccttttg caagctcaca gcaattctga taattatagt
      901 ccctggagtt atgcagctaa ttaaaggtca aacgcagaac tttaaagacg ccttttcagg
      961 aagagattca agtattacgc ggttgccact ggctttttat tatggaatgt atgcatatgc
     1021 tggctggttt tacctcaact ttgttactga agaagtagaa aaccctgaaa aaaccattcc
     1081 ccttgcaata tgtatatcca tggccattgt caccattggc tatgtgctga caaatgtggc
     1141 ctactttacg accattaatg ctgaggagct gctgctttca aatgcagtgg cagtgacctt
     1201 ttctgagcgg ctactgggaa atttctcatt agcagttccg atctttgttg ccctctcctg
     1261 ctttggctcc atgaacggtg gtgtgtttgc tgtctccagg ttattctatg ttgcgtctcg
     1321 agagggtcac cttccagaaa tcctctccat gattcatgtc cgcaagcaca ctcctctacc
     1381 agctgttatt gttttgcacc ctttgacaat gataatgctc ttctctggag acctcgacag
     1441 tcttttgaat ttcctcagtt ttgccaggtg gctttttatt gggctggcag ttgctgggct
     1501 gatttatctt cgatacaaat gcccagatat gcatcgtcct ttcaaggtgc cactgttcat
     1561 cccagctttg ttttccttca catgcctctt catggttgcc ctttccctct attcggaccc
     1621 atttagtaca gggattggct tcgtcatcac tctgactgga gtccctgcgt attatctctt
     1681 tattatatgg gacaagaaac ccaggtggtt tagaataatg tcggagaaaa taaccagaac
     1741 attacaaata atactggaag ttgtaccaga agaagataag ttatgaacta atggacttga
     1801 gatcttggca atctgcccaa ggggagacac aaaataggga tttttacttc attttctgaa
     1861 agtctagaga attacaactt tggtgataaa caaaaggagt cagttatttt tattcatata
     1921 ttttagcata ttcgaactaa tttctaagaa atttagttat aactctatgt agttatagaa
     1981 agtgaatatg cagttattct atgagtcgca caattcttga gtctctgata cctacctatt
     2041 ggggttagga gaaaagacta gacaattact atgtggtcat tctctacaac atatgttagc
     2101 acggcaaaga accttcaaat tgaagactga gatttttctg tatatatggg ttttgtaaag
     2161 atggttttac acactataga tgtctatact gtgaaaagtg ttttcaattc tgaaaaaaag
     2221 catacatcat gattatggca aagaggagag aaagaaattt attttacatt gacattgcat
     2281 tgcttcccct tagataccaa tttagataac aaacactcat gctttaatgg attataccca
     2341 gagcactttg aacaaaggtc agtggggatt gttgaataca ttaaagaaga gtttctaggg
     2401 gctactgttt atgagacaca tccaggagtt atgtttaagt aaaaatcctt gagaatttat
     2461 tatgtcagat gttttttcat tcattatcag gaagttttag ttatctgtca tttttttttt
     2521 tcacatcagt ttgatcagga aagtgtataa cacatcttag agcaagagtt agtttggtat
     2581 taaatcctca ttagaacaac cacctgtttc actaataact tacccctgat gagtctatct
     2641 aaacatatgc attttaagcc ttcaaattac attatcaaca tgagagaaat caccaacaaa
     2701 gaagatgttc aaaataatag tcccatatct gtaatcatat ctacatgcaa tgttagtaat
     2761 tctgaagttt tttaaattta tggctatttt tacacgatga tgaattttga cagtttgtgc
     2821 attttcttta tacattttat attcttctgt taaaatatct cttcagatga aactgtccag
     2881 attaattagg aaaaggcata tattaacata aaaattgcaa aagaaatgtc gctgtaaata
     2941 agatttacaa ctgatgtttc tagaaaattt ccacttctat atctaggctt tgtcagtaat
     3001 ttccacacct taattatcat tcaacttgca aaagagacaa ctgataagaa gaaaattgaa
     3061 atgagaatct gtggataagt gtttgtgttc agaagatgtt gttttgccag tattagaaaa
     3121 tactgtgagc cgggcatggt ggcttacatc tgtaatccca gcactttggg aggctgaggg
     3181 ggtggatcac ctgaggtcgg gagttctaga ccagcctgac caacatggag aaaccccatc
     3241 tctactaaaa atacaaaatt agctgggcat ggtggcacat gctggtaatc tcagctattg
     3301 aggaggctga ggcaggagaa ttgcttgaac ccgggaggcg gaggttgcag tgagccaaga
     3361 ttgcaccact gtactccagc ctgggtgaca aagtcagact ccatctccaa aaaaaaaaga
     3421 ttatatatat atatatatgt gtgtgtatgt gtgtgtgtgt gtgtgtgtgt atatatatat
     3481 atatatatat acacacacac acacacactt tttatatata tatatatata tatatagtgg
     3541 aacttacaaa tgagagtaat ataatgatga aattttgaac tgttatttat aaacatctaa
     3601 ggtaaaatgg ttagtcatgg ccagagtatg tttcatcctt taatttttgt ccatttgaaa
     3661 ataaggattt ttgaaagaat tataccaatt aaaattatta aaggcaaaca tagaattcat
     3721 aaaaaattgt ccaaagtaga aatgatgacc tataatttgg agcatttcca attcagtaat
     3781 ttcaattttg ctcttgaaaa catttaatat atatccaaga ctgacatttc tttagctgaa
     3841 cctaacgttt gggtctctga gtgaatttat aataactcct tccttcctta gcatagggtt
     3901 ttcaaaattt gatttataat tcctatttcc agtaaatatt gttcatttgt ccacatctct
     3961 ccctatgata tgttgctgga ggtaagaatt tctttcatat tcctattttt tttttcccca
     4021 tagactaggc tcatagaatt taaacaagca aattttcctg agctttttct tgccaaatga
     4081 aagaagactg gtaaattctc atagagaggt ttgtgtagtt cttggctctt cctggggtta
     4141 atgtgcttat attcacagtg gcaaattggt ctcagacttt aatttattta tttttgattt
     4201 gaatttctct ttaaaagtat caatttaaaa ggtaactaga attattcttt ctcattttca
     4261 aaagtgattt ttgcattatt aaatttccct gccattgtaa tgccatttca cgcagaaaaa
     4321 aagtcagcca gtaattaaga aaaaaagtga tggagattaa gtagtatttt ggcttatttt
     4381 taggactcat catgagaaga cacagttcct ttaatcagga aattaatatc cataattttc
     4441 actcaaaatt gcagtatgta aagcagattc tcaaaaactc tcctgaacac ttatttatat
     4501 atatgttttt atataagtaa aatttttctc atatttttat acgatatgca cacacacaca
     4561 tacatgcaca tactacttac tacatgttct gtacttgtac tttgtaccat gcatattcaa
     4621 atgtttatat acataagttt attataacat aaacagtaaa agtaatgaat actgtttaaa
     4681 ataactaata tagtattttt taatttttgt ggggatggat tctcaaatac ttgtgatttt
     4741 aaaagattct aaagctaaaa cacaacttga ttttaaaaag aatgattctc cttacacaat
     4801 tataaatatt tgcagtaaat attttcctta taatactgtt ttgaccccat ttaaaaagta
     4861 ttagattata ttcctttgat ccaatgaaaa ctgaacctta taaatggtta gctgaaagta
     4921 gaccttattc ttgtccttct ttagaagagt aaagatttgt cctagggaag atggctgact
     4981 tcggttccca acatgcgtat gcatttagac tgtagctcct cagccctgtg gacacaaaat
     5041 ttggacagct tattaggtta cgttagcaat gcatgacggt ttctccaaca ctaagatatt
     5101 cacgttgaaa cagatttcct gttcgtctta tgtgtctggt aaaattgttt ccccaattac
     5161 aatttgacat atcaatagag ggttaacaag agtataatta cataacagaa ttcctcatga
     5221 actgtaatca gtctacagga aaatcattat tttatcttga tttgcagatg aatatactgc
     5281 taagaaaggg agcaactctg acctttgtta aagttgatct tttgtaattg aggtataagg
     5341 tatgaaaaga taaaaaaccg aaggccagag aatcaggaaa tgaaagatag tatggactga
     5401 aggtaacaat attttaatgt tatgcaatat agtcagagaa atattaaaaa ttagttgttt
     5461 gctgtgcata ggtggatctc gcaggaagct aatgaaacct aagcttcagt gcctctcact
     5521 tagacatgtt ccattcgagg tcctgaacct aactttgtat taggaattct gtactaattt
     5581 tgttgaagaa gaccagcaaa gttgtgtaca cttctacccc cacaaaatct gcattgtcca
     5641 tgtgagtaaa gtaaaataat tcctgttatt tttttctgtt agaaataagt atggaggata
     5701 tgtttttaaa aatttatgag ttaattgaaa tatccatata taacaagtga ctttctcaca
     5761 atatatatga tgtgatatat agggagatag tttcactttc atcatatttt atacgttgat
     5821 tctgaactat agaaaaataa taaatgggat tttaattata gctcttagtt gggaaagaaa
     5881 tatagagaga tgtgggattt gaatgcccat gaaagacatt ttattttact tgaatatatt
     5941 cttgcttcac tttaccctcc ataatatgtt gtacattagt gctgatcaag tttacagagt
     6001 tacattttgc tttcctaacc attcagtcag gaattaaaat atggcattgt ataacaactg
     6061 ggaagaagct catagtggat ataaattaga gtagataatg ggtcaccttg atagcctctg
     6121 tttacattac ttgtatatgg gcaaaataat tattacctat acgtgtattt aagcttaatt
     6181 ttcatataaa cagtattttt aatctatgtt aaaatagata atatctaaaa gtgtgatctc
     6241 taggtagtcc ttagtttatt agtactgtac ttcaaaaaga tttttaaata ggtccggcac
     6301 ggtggctcat gcctgtaatc ccagcacttt gggaggctga ggcgggcgaa tcacctgagg
     6361 tcaggagttc gagatcagcc tggccaacat ggtgaaaccc tgtctcaact aaaaatataa
     6421 aaattagccg ggcgtggtgg caggcgcctg taatcccagc tactcgggag gctgaggcag
     6481 gagaatcact tgaacccaag gggcagaagc tgcagttagc caagatcgca tcattgcact
     6541 ccagcctagg ggacaagagc gcgagacttc atctcaaaaa aaaaaaaaaa aaaaaaaaaa
     6601 gatttttaaa taatagctaa aggtatgctc tctaggtcat ccttagttta ttagtactgt
     6661 acttaaaaat tattttttaa tagtcaattt tgggagataa ttatttcttt ccttatattt
     6721 tccaattagt tggtgtctaa aaataaatgt tttgtctaat tttagatcag gtatacattc
     6781 acaaaagcat aaatcatagt ctcacaggaa attcaccaat tttccatatg tcgtgagata
     6841 actgtccttt ctacaacctc ataacaatga atttatataa ttacctagat tttcttagtg
     6901 tgaatctacc cattagtttt attttcttgg tagttatttt tttccctcct ctctgttact
     6961 attggcctta aaatacacag aggacggtta cagtgtccta atagctgtta catgtgtgtg
     7021 tttcagcgta cttgaatcaa gtgtacattt atagtaccaa taaccgcctt tacagcttta
     7081 cagttaacaa ttctctcaca aaactgtaga gcattaggca tctgagagcc atagagggcc
     7141 aactttgttc cagagtgaac atgctttttt tcctcaacat atacactact gatttttttt
     7201 aaaagtatga ctttcaagtg aattaatgta ttggttagga gaactgcttg ctaagtcctt
     7261 attacctctt gttaaagcct cagaaggccg tgctgaaagc cagaggggaa aaaaagagta
     7321 atgcacaggt atctcttttg cagtggtgac tgtattttga gtaccttgtg tgacagggta
     7381 ttattacagc atcttgtggg aaaacctatt aggcctttgc atgttaaagc tgtataattt
     7441 gttgggttgt gagtggtctg acttaaatgt gtattataaa atttagacat caaattttcc
     7501 tactaactaa ctttattaga tgcatacttg gaagcacagt catatcacac tgggaggcaa
     7561 tgcaatgtgg ttacctggtc ctaggtttga actgtcttat ttcaaaagat ttctgaatta
     7621 atttttccct agaatttctc cttcattcca aagtacaaac atactttgaa gaatgaaaca
     7681 gattgttccc atgaatgtat gctcatactc gactagaaac gatctatgtt aaatgactgt
     7741 gtatatgaat tatttcaagt actaccccaa ataactttct tattgctctg aaagaagaaa
     7801 agcaatgtaa atcactatga ttattgcaca aacaaccaga attctccaac aattttaagt
     7861 aatctgatcc tcttcttgga gaaaattgtt acctaatagt ttttccttat gaatgttatt
     7921 actactggta taaatcaaat ttctataaat ttcctactta agtcttaaga actgggttct
     7981 tcctttgatg ttattcatgt tcagaaagga aacaacactt tactctttta ggacaattcc
     8041 tagaatctat agtagtatca ggatatattt tgctttaaaa tatattttgg ttattttgaa
     8101 tacagacatt ggctccaaat tttcatcttt gcacaatagt atgacttttc actagaactt
     8161 ctcaacattt gggaactttg caaatatgag catcatatgt gttaaggctg tatcatttaa
     8221 tgctatgaga tacattgttt tctccctatg ccaaacaggt gaacaaacgt agttgttttt
     8281 tactgatact aaatgttggc tacctgtgat tttatagtat gcacatgtca gaaaaaggca
     8341 agacaaatgg cctcttgtac tgaatacttc ggcaaactta ttgggtcttc attttctgac
     8401 agacaggatt tgactcaata tttgtagagc ttgcgtagaa tggattacat ggtagtgatg
     8461 cactggtaga aatggttttt agttattgac tcagaattca tctcaggatg aatcttttat
     8521 gtctttttat tgtaagcata tctgaattta ctttataaag atggttttag aaagctttgt
     8581 ctaaaaattt ggcctaggaa tggtaacttc attttcagtt gccaaggggt agaaaaataa
     8641 tatgtgtgtt gttatgttta tgttaacata ttattaggta ctatctatga atgtatttaa
     8701 atatttttca tattctgtga caagcattta taatttgcaa caagtggagt ccatttagcc
     8761 cagtgggaaa gtcttggaac tcaggttacc cttgaaggat atgctggcag ccatctcttt
     8821 gatctgtgct taaactgtaa tttatagacc agctaaatcc ctaacttgga tctggaatgc
     8881 attagttatg accttgtacc attcccagaa tttcaggggc atcgtgggtt tggtctagtg
     8941 attgaaaaca caagaacaga gagatccagc tgaaaaagag tgatcctcaa tatcctaact
     9001 aactggtcct caactcaagc agagtttctt cactctggca ctgtgatcat gaaacttagt
     9061 agaggggatt gtgtgtattt tatacaaatt taatacaatg tcttacattg ataaaattct
     9121 taaagagcaa aactgcattt tatttctgca tccacattcc aatcatatta gaactaagat
     9181 atttatctat gaagatataa atggtgcaga gagactttca tctgtggatt gcgttgtttc
     9241 ttagggttcc tagcactgat gcctgcacaa gcatgtgata tgtgaaataa aatggattct
     9301 tctatagcta aatgagttcc ctctggggag agttctggta ctgcaatcac aatgccagat
     9361 ggtgtttatg ggctatttgt gtaagtaagt ggtaagatgc tatgaagtaa gtgtgtttgt
     9421 tttcatctta tggaaactct tgatgcatgt gcttttgtat ggaataaatt ttggtgcaat
     9481 atgatgtcat tcaactttgc attgaattga attttggttg tatttatatg tattatacct
     9541 gtcacgcttc tagttgcttc aaccatttta taaccatttt tgtacatatt ttacttgaaa
     9601 atattttaaa tggaaattta aataaacatt tgatagttta cataa
//
