LOCUS       NM_020208               5425 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens solute carrier family 6 member 20 (SLC6A20),
            transcript variant 1, mRNA.
ACCESSION   NM_020208
VERSION     NM_020208.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Kousathanas,A., Pairo-Castineira,E., Rawlik,K., Stuckey,A.,
            Odhams,C.A., Walker,S., Russell,C.D., Malinauskas,T., Wu,Y.,
            Millar,J., Shen,X., Elliott,K.S., Griffiths,F., Oosthuyzen,W.,
            Morrice,K., Keating,S., Wang,B., Rhodes,D., Klaric,L., Zechner,M.,
            Parkinson,N., Siddiq,A., Goddard,P., Donovan,S., Maslove,D.,
            Nichol,A., Semple,M.G., Zainy,T., Maleady-Crowe,F., Todd,L.,
            Salehi,S., Knight,J., Elgar,G., Chan,G., Arumugam,P., Patch,C.,
            Rendon,A., Bentley,D., Kingsley,C., Kosmicki,J.A., Horowitz,J.E.,
            Baras,A., Abecasis,G.R., Ferreira,M.A.R., Justice,A., Mirshahi,T.,
            Oetjens,M., Rader,D.J., Ritchie,M.D., Verma,A., Fowler,T.A.,
            Shankar-Hari,M., Summers,C., Hinds,C., Horby,P., Ling,L.,
            McAuley,D., Montgomery,H., Openshaw,P.J.M., Elliott,P., Walsh,T.,
            Tenesa,A., Fawkes,A., Murphy,L., Rowan,K., Ponting,C.P., Vitart,V.,
            Wilson,J.F., Yang,J., Bretherick,A.D., Scott,R.H., Hendry,S.C.,
            Moutsianas,L., Law,A., Caulfield,M.J. and Baillie,J.K.
  CONSRTM   GenOMICC investigators; 23andMe investigators; COVID-19 Human
            Genetics Initiative
  TITLE     Whole-genome sequencing reveals host factors underlying critical
            COVID-19
  JOURNAL   Nature 607 (7917), 97-103 (2022)
   PUBMED   35255492
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Semiz,S.
  TITLE     SIT1 transporter as a potential novel target in treatment of
            COVID-19
  JOURNAL   Biomol Concepts 12 (1), 156-163 (2021)
   PUBMED   34969185
  REMARK    GeneRIF: SIT1 transporter as a potential novel target in treatment
            of COVID-19.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5425)
  AUTHORS   Kasela,S., Daniloski,Z., Bollepalli,S., Jordan,T.X., tenOever,B.R.,
            Sanjana,N.E. and Lappalainen,T.
  TITLE     Integrative approach identifies SLC6A20 and CXCR6 as putative
            causal genes for the COVID-19 GWAS signal in the 3p21.31 locus
  JOURNAL   Genome Biol 22 (1), 242 (2021)
   PUBMED   34425859
  REMARK    GeneRIF: Integrative approach identifies SLC6A20 and CXCR6 as
            putative causal genes for the COVID-19 GWAS signal in the 3p21.31
            locus.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5425)
  AUTHORS   Yao,Y., Ye,F., Li,K., Xu,P., Tan,W., Feng,Q. and Rao,S.
  TITLE     Genome and epigenome editing identify CCR9 and SLC6A20 as target
            genes at the 3p21.31 locus associated with severe COVID-19
  JOURNAL   Signal Transduct Target Ther 6 (1), 85 (2021)
   PUBMED   33619245
  REMARK    GeneRIF: Genome and epigenome editing identify CCR9 and SLC6A20 as
            target genes at the 3p21.31 locus associated with severe COVID-19.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5425)
  AUTHORS   Bae,M., Roh,J.D., Kim,Y., Kim,S.S., Han,H.M., Yang,E., Kang,H.,
            Lee,S., Kim,J.Y., Kang,R., Jung,H., Yoo,T., Kim,H., Kim,D., Oh,H.,
            Han,S., Kim,D., Han,J., Bae,Y.C., Kim,H., Ahn,S., Chan,A.M.,
            Lee,D., Kim,J.W. and Kim,E.
  TITLE     SLC6A20 transporter: a novel regulator of brain glycine homeostasis
            and NMDAR function
  JOURNAL   EMBO Mol Med 13 (2), e12632 (2021)
   PUBMED   33428810
  REMARK    Erratum:[EMBO Mol Med. 2024 Oct;16(10):2653. doi:
            10.1038/s44321-024-00125-y.. PMID: 39242973]
REFERENCE   6  (bases 1 to 5425)
  AUTHORS   Takanaga,H., Mackenzie,B., Suzuki,Y. and Hediger,M.A.
  TITLE     Identification of mammalian proline transporter SIT1 (SLC6A20) with
            characteristics of classical system imino
  JOURNAL   J Biol Chem 280 (10), 8974-8984 (2005)
   PUBMED   15632147
REFERENCE   7  (bases 1 to 5425)
  AUTHORS   Kanei-Ishii,C., Nomura,T., Tanikawa,J., Ichikawa-Iwata,E. and
            Ishii,S.
  TITLE     Differential sensitivity of v-Myb and c-Myb to Wnt-1-induced
            protein degradation
  JOURNAL   J Biol Chem 279 (43), 44582-44589 (2004)
   PUBMED   15308626
REFERENCE   8  (bases 1 to 5425)
  AUTHORS   Kiss,H., Kedra,D., Kiss,C., Kost-Alimova,M., Yang,Y., Klein,G.,
            Imreh,S. and Dumanski,J.P.
  TITLE     The LZTFL1 gene is a part of a transcriptional map covering 250 kb
            within the common eliminated region 1 (C3CER1) in 3p21.3
  JOURNAL   Genomics 73 (1), 10-19 (2001)
   PUBMED   11352561
REFERENCE   9  (bases 1 to 5425)
  AUTHORS   Nash,S.R., Giros,B., Kingsmore,S.F., Kim,K.M., el-Mestikawy,S.,
            Dong,Q., Fumagalli,F., Seldin,M.F. and Caron,M.G.
  TITLE     Cloning, gene structure and genomic localization of an orphan
            transporter from mouse kidney with six alternatively-spliced
            isoforms
  JOURNAL   Recept Channels 6 (2), 113-128 (1998)
   PUBMED   9932288
REFERENCE   10 (bases 1 to 5425)
  AUTHORS   Stevens,B.R. and Wright,E.M.
  TITLE     Kinetics of the intestinal brush border proline (Imino) carrier
  JOURNAL   J Biol Chem 262 (14), 6546-6551 (1987)
   PUBMED   3571270
  REMARK    GeneRIF: Characterization and substrate specificity of the Na+
            coupled IMINO transport system in apical brush border membranes of
            epithelial cells. Identically found in kidney proximal tubule.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DR006419.1, AJ276207.1,
            AC098476.2 and EL949345.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_020208.3.
            
            Summary: Transport of small hydrophilic substances across cell
            membranes is mediated by substrate-specific transporter proteins
            which have been classified into several families of related genes.
            The protein encoded by this gene belongs to the
            sodium:neurotransmitter symporter (SNF) family and functions as a
            proline transporter expressed in kidney and small intestine.
            Mutations in this gene are associated with Hyperglycinuria and
            Iminoglycinuria. [provided by RefSeq, Jul 2020].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ276207.1, BC126197.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2144835 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: locus in the vicinity of
                                      disease-associated variant(s)
            MANE Ensembl match     :: ENST00000358525.9/ ENSP00000346298.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-66                DR006419.1         15-80
            67-533              AJ276207.1         1-467
            534-534             AC098476.2         126599-126599       c
            535-2494            AJ276207.1         469-2428
            2495-4904           AC098476.2         106643-109052       c
            4905-5425           EL949345.1         9-529
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..5425
                     /gene="SLC6A20"
                     /gene_synonym="IMINO; SIT1; XT3; Xtrp3"
                     /note="solute carrier family 6 member 20"
                     /db_xref="GeneID:54716"
                     /db_xref="HGNC:HGNC:30927"
                     /db_xref="MIM:605616"
     exon            1..238
                     /gene="SLC6A20"
                     /gene_synonym="IMINO; SIT1; XT3; Xtrp3"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..1896
                     /gene="SLC6A20"
                     /gene_synonym="IMINO; SIT1; XT3; Xtrp3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     orphan transporter XT3; sodium/imino-acid transporter 1;
                     solute carrier family 6 (proline IMINO transporter),
                     member 20; solute carrier family 6 (neurotransmitter
                     transporter), member 20; X transporter protein 3; sodium-
                     and chloride-dependent transporter XTRP3; neurotransmitter
                     transporter RB21A; transporter rB21A homolog;
                     sodium-dependent imino acid transporter 1"
                     /codon_start=1
                     /product="sodium- and chloride-dependent transporter XTRP3
                     isoform 1"
                     /protein_id="NP_064593.1"
                     /db_xref="CCDS:CCDS43077.1"
                     /db_xref="GeneID:54716"
                     /db_xref="HGNC:HGNC:30927"
                     /db_xref="MIM:605616"
                     /translation="MEKARPLWANSLQFVFACISYAVGLGNVWRFPYLCQMYGGGSFL
                     VPYIIMLIVEGMPLLYLELAVGQRMRQGSIGAWRTISPYLSGVGVASVVVSFFLSMYY
                     NVINAWAFWYLFHSFQDPLPWSVCPLNGNHTGYDEECEKASSTQYFWYRKTLNISPSL
                     QENGGVQWEPALCLLLAWLVVYLCILRGTESTGKVVYFTASLPYCVLIIYLIRGLTLH
                     GATNGLMYMFTPKIEQLANPKAWINAATQIFFSLGLGFGSLIAFASYNEPSNNCQKHA
                     IIVSLINSFTSIFASIVTFSIYGFKATFNYENCLKKVSLLLTNTFDLEDGFLTASNLE
                     QVKGYLASAYPSKYSEMFPQIKNCSLESELDTAVQGTGLAFIVYTEAIKNMEVSQLWS
                     VLYFFMLLMLGIGSMLGNTAAILTPLTDSKIISSHLPKEAISGLVCLVNCAIGMVFTM
                     EAGNYWFDIFNDYAATLSLLLIVLVETIAVCYVYGLRRFESDLKAMTGRAVSWYWKVM
                     WAGVSPLLIVSLFVFYLSDYILTGTLKYQAWDASQGQLVTKDYPAYALAVIGLLVASS
                     TMCIPLAALGTFVQRRLKRGDADPVA"
     exon            239..379
                     /gene="SLC6A20"
                     /gene_synonym="IMINO; SIT1; XT3; Xtrp3"
                     /inference="alignment:Splign:2.1.0"
     exon            380..471
                     /gene="SLC6A20"
                     /gene_synonym="IMINO; SIT1; XT3; Xtrp3"
                     /inference="alignment:Splign:2.1.0"
     exon            472..699
                     /gene="SLC6A20"
                     /gene_synonym="IMINO; SIT1; XT3; Xtrp3"
                     /inference="alignment:Splign:2.1.0"
     exon            700..810
                     /gene="SLC6A20"
                     /gene_synonym="IMINO; SIT1; XT3; Xtrp3"
                     /inference="alignment:Splign:2.1.0"
     exon            811..1052
                     /gene="SLC6A20"
                     /gene_synonym="IMINO; SIT1; XT3; Xtrp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1215
                     /gene="SLC6A20"
                     /gene_synonym="IMINO; SIT1; XT3; Xtrp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1420
                     /gene="SLC6A20"
                     /gene_synonym="IMINO; SIT1; XT3; Xtrp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1580
                     /gene="SLC6A20"
                     /gene_synonym="IMINO; SIT1; XT3; Xtrp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1746
                     /gene="SLC6A20"
                     /gene_synonym="IMINO; SIT1; XT3; Xtrp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1747..5425
                     /gene="SLC6A20"
                     /gene_synonym="IMINO; SIT1; XT3; Xtrp3"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      4051
                     /gene="SLC6A20"
                     /gene_synonym="IMINO; SIT1; XT3; Xtrp3"
                     /note="major polyA site"
     regulatory      5398..5403
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC6A20"
                     /gene_synonym="IMINO; SIT1; XT3; Xtrp3"
                     /note="hexamer: AATAAA"
     polyA_site      5425
                     /gene="SLC6A20"
                     /gene_synonym="IMINO; SIT1; XT3; Xtrp3"
                     /note="major polyA site"
ORIGIN      
        1 ggaggcgcgg ggccaccccc aagccgagcc ggggtgggac ggcgcgcctg gcgaccgcgc
       61 actgagactg ccgtgccgga cccccgagcc ggagccgagc gcgccgaggc cggggccatg
      121 gagaaagcgc ggccgctgtg ggccaactcg ctacagttcg tgttcgcctg catctcgtac
      181 gccgtgggcc tgggcaacgt gtggcgattc ccgtacctgt gccagatgta cggcggaggt
      241 agtttcctgg tcccctacat catcatgctt atcgtggagg gaatgccgct cttgtacctg
      301 gaactggctg tggggcagcg catgcggcag ggcagcatcg gcgcctggag gaccatcagc
      361 ccgtacctca gtggtgtcgg ggtcgccagc gtggtggtct ctttcttcct ctccatgtac
      421 tacaacgtca tcaacgcctg ggccttctgg tacctcttcc actccttcca ggatcccctg
      481 ccgtggtctg tctgcccact gaatggtaac cacacgggct acgatgagga gtgtgagaag
      541 gcgtcctcca cacagtactt ctggtacagg aaaaccctca atatctcgcc gtccctccag
      601 gagaacgggg gtgtgcagtg ggagccggcg ctgtgcctcc tcctggcctg gctggtggtg
      661 tacctgtgca tcctgcgtgg caccgagtcc actggcaagg tggtgtattt cacggcgtca
      721 ctgccctatt gcgtgctcat catctacctc atcaggggcc tcacgctcca cggagccacc
      781 aatggcctca tgtacatgtt cactcccaag atagagcagc tggccaaccc caaggcctgg
      841 atcaatgcag ccacccagat cttcttctca cttggcctgg gcttcggcag cctgatcgcc
      901 ttcgccagct acaatgagcc atccaacaac tgccagaagc acgccatcat cgtgtccctc
      961 atcaacagct tcacctccat atttgccagc attgtcacct tctccatcta tggcttcaag
     1021 gccaccttca attatgaaaa ctgcttgaag aaggtgagtc tgctgctgac caacactttt
     1081 gaccttgaag atggcttttt gacagccagc aacctggagc aggtgaaggg ctacctcgca
     1141 tctgcctacc caagcaaata cagcgagatg ttcccgcaaa tcaaaaactg cagcttggaa
     1201 tcggagctag acacggccgt ccagggcact ggcctggcat tcatcgtcta cacagaggcc
     1261 attaaaaaca tggaggtgtc ccagctgtgg tcggtgctct acttcttcat gctgctgatg
     1321 ctgggcattg ggagcatgct ggggaacaca gcggccatcc tcacccctct gacagacagc
     1381 aagatcatct ccagccacct gcccaaggag gccatctcag gtctggtgtg ccttgtcaac
     1441 tgtgccattg gcatggtgtt cacgatggag gctgggaact actggtttga catattcaac
     1501 gactacgcgg ccacactgtc cctgctgctc atcgtgctgg tggagacgat tgccgtgtgc
     1561 tacgtgtacg ggctgaggag atttgaaagt gaccttaagg ccatgaccgg ccgagctgtg
     1621 agctggtact ggaaggtgat gtgggctggc gtaagcccac tgctgattgt cagcctcttt
     1681 gtcttctacc tgagcgacta catcctcacg gggaccctga agtatcaagc ctgggacgcc
     1741 tcccagggcc agctcgtgac caaagattac ccggcctatg cactggctgt catcgggctg
     1801 cttgtggcct cctccaccat gtgcatcccc ctggcggccc tggggacttt tgttcagcgt
     1861 cgcctcaaga ggggagacgc agaccccgtg gcctgagatg tgggcttccc agccgctcac
     1921 ggttttacag atactattta caggcggaaa ctcctcggct gctttttcaa atgcttaagc
     1981 caggagtgct cagcccatca acttcctgag tgtctaaaga agatgaggaa ggtgtgcagg
     2041 aagaaaactc ccttgggaga acgcacaccc tcccgtggtg gctgttcctc cctgtcacct
     2101 gcctcctcat catggaaggg ggtgggctat gaaagccggt ctcaaagata actgcatcct
     2161 tcattccagg aaagccctag aattagggca cattgcaaac tgaaatatga ctataattct
     2221 tatgggacca aatttaagca atttttgttt ttggctgaag agacaccaaa atattagagg
     2281 acaaatattt ttagatccat ttaaggagtt ttgaagtgcc taagatgacc tatttgtcag
     2341 tggtgcaaaa ttaattctct tcttttttga gttgtagtga atatgcaatt tctgtgttcc
     2401 ccttccaccc tttaaatctt aggatgacaa gttataaaga aagaagatct ttgtctggga
     2461 cccccaaagg gatcctttct ctaaggtctc tgacggtggg tccaggacca gacctctcta
     2521 caaaaaattg ccccaactac agtttgcaac cccaaaccac attagaagtc tgtgcagaca
     2581 tccctccgtg gtgtgtgtct tggtgcattg gaaaaggagt caggagccac tgtgaggtga
     2641 gaatgaaagt ggatctcagc tgggcacggt ggctcacgcc tgtaatccta gcaccttggg
     2701 ggtcaaggtg ggtggatcac ttgaggtcag gagtttgagg ccagcctggc caacatggcg
     2761 aaaccccatt tctactaaaa atacaaaaaa attagctggg agtggtggca taggcctgta
     2821 attccagcta ctctggctgc tgaggcacaa gaatcatttg aacctaggag gtggcggttg
     2881 ctgtgagcca agatcatgcc actccactcc agtctgggcg acagagcaag actctgactc
     2941 aaaaaaaaaa aaaaaaaaaa gtggctctga ctttcccata ggcttaatat tacatttgga
     3001 catcgtggcc aactctatca agaccacaaa taccacttac aattaatttt aaattattca
     3061 tctgtacata gttttctaaa atgtatataa ttcaaacaga gcatcttgta actgaagaca
     3121 caccatatct atgatatcgc attagtccat gtggggaaaa gaaagatcag attgttactg
     3181 tgtctgtgta gaaaaggaag acataagaaa ctccattttg atctgtacta agaaaaattg
     3241 tttctgcttt gagatgttgt tagcctataa ctttagcccc aactctgtgc tcacagaaac
     3301 atgcgctgta atgaatcaaa gtttaatgga tttagggctg tgcaggatgt gccttgttaa
     3361 caatatgttt gcaggctgta tgcttggtag aagtcatcgc cattctccat tctcgattaa
     3421 ccagggacac aatgcacagc agaaagctgc agggacctct gcccaagaaa gcctgggtat
     3481 tgtccaaggt ttccccccca ctgagacagc ctgagatatg gcctcatggg aagggaaaga
     3541 cctgactgtc ccccagcccg acacctgtaa agggtcggtg ctgaggagga atagtgaagg
     3601 agggaggcct ctttgcagtt gagataagag gaaggcttct gtctcctgct tgtccctggt
     3661 aatggaatgt ctcggtgtaa aaagctgacc attcccattc gttctattct gagataggag
     3721 aaaaccgccc tgtggctgga ggtgagatat gctggcagca atactgctct gttactcctt
     3781 gctacactga gatgtttggg taaagagaaa cataaatcta gcctacgtgc acatctgggc
     3841 acagtacctt tccttgaact tattcgtgat acagattcct ttgctcacat gtttccctgc
     3901 tgaccttctt cccacctgtt gccctgctac actcccctcg ctaagacagt aaaaataatg
     3961 atcaataaat actgagggaa ctcagaggcc agcgccggtg cgggtcctcc acatgctgag
     4021 cgccggtcct gggcccactg ttctttctct atactttgtc tctgtgtctt atttcttttc
     4081 tcaaaaaaat ttttcttttt ttaactgcaa taaagtaggt cgttacccct aaagtgagcg
     4141 agtcccttcc tgctctaacg tccagtgctt ccttgagatc atggaattct ggagagtttc
     4201 tgtggatata tttgcacaga tatctttgaa gacacaaaca ctcacctggg acgttggccg
     4261 gagatgtctt tttatttttg tgctgtaaaa ttctcttaca gcaaaaatag gctttagaaa
     4321 ggtcttctac tgtcttcagc aaccatctca tcttccagct tcacctgatt gtccagttat
     4381 catacatttg actttcaaat gtatgaacca gcatgtaccc catggattta atcttatcta
     4441 ccccgtggat tcaatcttct tatcagaagg ttcttttatg tcaaaaaacc tgctgtcaag
     4501 gcttgaagag ccggcacact caatggcaaa cacagcaccg agtctgctct gaatcctgga
     4561 ggatctggcc ctcctctcaa tccccactca cagtcaccgt cttacaactc agggccacct
     4621 gggatcagtc atcagtcagg gtgcgtaagc cttgaatacc aggtagcctc aggagtgaaa
     4681 agataaatgt cctagatcat taccttattc agtgtcccca ccttgcagcg cattccaacc
     4741 acctgggagc atttaaaact ccagatgccc acaccacacc ctggggccac ccatcagacc
     4801 ttctggaagc aagacctggg cctccatggc cccaaaaact ccctaggtga tccgatgtgc
     4861 agccaaatct gagaggcccc atttaaaaaa gaaagaacat gggtggtaca ttgaggagta
     4921 tttacatttt ataaaatgac ttaaaaattt gaaggcattt ttgagcattt ccaattatat
     4981 ggaagagtta cttctacgga atagtttttg ctcatggaac tcaaacagat gaagcaccac
     5041 tgttacagaa taatgtgctc cagatgaaaa tgtctcgttt ctgtgaattt catgaagagc
     5101 agaacatttc tcaagaatcc tcttgagcca gtaatcaatc ctgtctcaaa aaatgttctt
     5161 tgccatttcc tagatactgc acaaaagtgg ccatgtcgac atttgtccac ccaccctcca
     5221 ataagctgga gcgacaaagg gacattccat ccctgtaccc ttagtggtag ccatgacacg
     5281 atggccagat catggactcc ggaaagcttt ctgtttttac tggaaacata gcaaaccttg
     5341 atttagctcc aagaaattga gtagggaaat atttgttttt tagcaattgt catagtaaat
     5401 aaaactctaa gcaagtgtat ctaca
//
