LOCUS       NM_178498               6285 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens solute carrier family 5 member 12 (SLC5A12), mRNA.
ACCESSION   NM_178498 NM_001042366
VERSION     NM_178498.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Pucino,V., Certo,M., Bulusu,V., Cucchi,D., Goldmann,K.,
            Pontarini,E., Haas,R., Smith,J., Headland,S.E., Blighe,K.,
            Ruscica,M., Humby,F., Lewis,M.J., Kamphorst,J.J., Bombardieri,M.,
            Pitzalis,C. and Mauro,C.
  TITLE     Lactate Buildup at the Site of Chronic Inflammation Promotes
            Disease by Inducing CD4+ T Cell Metabolic Rewiring
  JOURNAL   Cell Metab 30 (6), 1055-1074 (2019)
   PUBMED   31708446
  REMARK    GeneRIF: Lactate Buildup at the Site of Chronic Inflammation
            Promotes Disease by Inducing CD4(+) T Cell Metabolic Rewiring.
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   Srivastava,S., Nakagawa,K., He,X., Kimura,T., Fukutomi,T.,
            Miyauchi,S., Sakurai,H. and Anzai,N.
  TITLE     Identification of the multivalent PDZ protein PDZK1 as a binding
            partner of sodium-coupled monocarboxylate transporter SMCT1
            (SLC5A8) and SMCT2 (SLC5A12)
  JOURNAL   J Physiol Sci 69 (2), 399-408 (2019)
   PUBMED   30604288
  REMARK    GeneRIF: Results indicate that the SMCT1-PDZK1 interaction thus
            plays an important role in both lactate handling as well as urate
            reabsorption in the human kidney.
REFERENCE   3  (bases 1 to 6285)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   4  (bases 1 to 6285)
  AUTHORS   Gopal,E., Umapathy,N.S., Martin,P.M., Ananth,S.,
            Gnana-Prakasam,J.P., Becker,H., Wagner,C.A., Ganapathy,V. and
            Prasad,P.D.
  TITLE     Cloning and functional characterization of human SMCT2 (SLC5A12)
            and expression pattern of the transporter in kidney
  JOURNAL   Biochim Biophys Acta 1768 (11), 2690-2697 (2007)
   PUBMED   17692818
  REMARK    GeneRIF: low-affinity transporter SMCT2 initiates lactate
            absorption in the early parts of the proximal tubule followed by
            the participation of the high-affinity transporter SMCT1 in the
            latter parts of the proximal tubule.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC083755.10, BM924715.1, AY299482.1, CN370367.1, CR747563.1 and
            AW614338.1.
            
            On Nov 22, 2018 this sequence version replaced NM_178498.3.
            
            Summary: Normal blood lactate is maintained at about 1.5 mM, and
            little filtered lactate is excreted in urine. Reabsorption of
            lactate is mediated by the low-affinity Na(+)-coupled lactate
            transporter SLC5A12 in the initial part of the proximal tubule and
            by the high-affinity Na(+)-coupled lactate transporter SLC5A8 (MIM
            608044) in the distal proximal tubule (Gopal et al., 2007 [PubMed
            17692818]).[supplied by OMIM, Dec 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY299482.1, DRR138523.455995.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968189,
                                           SAMEA1968540 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000396005.8/ ENSP00000379326.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-33                AC083755.10        60933-60965
            34-329              BM924715.1         20-315
            330-729             AY299482.1         6-405
            730-730             AC083755.10        70331-70331
            731-960             AY299482.1         407-636
            961-1554            CN370367.1         37-630
            1555-1758           AY299482.1         1231-1434
            1759-1759           AC083755.10        101876-101876
            1760-2109           CR747563.1         112-461
            2110-2213           AY299482.1         1786-1889
            2214-5943           AC083755.10        111902-115631
            5944-6285           AW614338.1         5-346               c
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p14.2"
     gene            1..6285
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /note="solute carrier family 5 member 12"
                     /db_xref="GeneID:159963"
                     /db_xref="HGNC:HGNC:28750"
                     /db_xref="MIM:612455"
     exon            1..685
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /inference="alignment:Splign:2.1.0"
     CDS             347..2203
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /note="sodium-iodide related cotransporter; low-affinity
                     sodium-lactate cotransporter; solute carrier family 5
                     (sodium/glucose cotransporter), member 12; electroneutral
                     sodium monocarboxylate cotransporter; solute carrier
                     family 5 (sodium/monocarboxylate cotransporter), member
                     12"
                     /codon_start=1
                     /product="sodium-coupled monocarboxylate transporter 2"
                     /protein_id="NP_848593.2"
                     /db_xref="CCDS:CCDS7860.2"
                     /db_xref="GeneID:159963"
                     /db_xref="HGNC:HGNC:28750"
                     /db_xref="MIM:612455"
                     /translation="MEVKNFAVWDYVVFAALFFISSGIGVFFAIKERKKATSREFLVG
                     GRQMSFGPVGLSLTASFMSAVTVLGTPSEVYRFGASFLVFFIAYLFVILLTSELFLPV
                     FYRSGITSTYEYLQLRFNKPVRYAATVIYIVQTILYTGVVVYAPALALNQVTGFDLWG
                     SVFATGIVCTFYCTLGGLKAVVWTDAFQMVVMIVGFLTVLIQGSTHAGGFHNVLEQST
                     NGSRLHIFDFDVDPLRRHTFWTITVGGTFTWLGIYGVNQSTIQRCISCKTEKHAKLAL
                     YFNLLGLWIILVCAVFSGLIMYSHFKDCDPWTSGIISAPDQLMPYFVMEIFATMPGLP
                     GLFVACAFSGTLSTVASSINALATVTFEDFVKSCFPHLSDKLSTWISKGLCLLFGVMC
                     TSMAVAASVMGGVVQASLSIHGMCGGPMLGLFSLGIVFPFVNWKGALGGLLTGITLSF
                     WVAIGAFIYPAPASKTWPLPLSTDQCIKSNVTATGPPVLSSRPGIADTWYSISYLYYS
                     AVGCLGCIVAGVIISLITGRQRGEDIQPLLIRPVCNLFCFWSKKYKTLCWCGVQHDSG
                     TEQENLENGSARKQGAESVLQNGLRRESLVHVPGYDPKDKSYNNMAFETTHF"
     exon            686..751
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /inference="alignment:Splign:2.1.0"
     exon            752..803
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /inference="alignment:Splign:2.1.0"
     exon            804..871
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /inference="alignment:Splign:2.1.0"
     exon            872..1026
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1167
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1297
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1386
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1387..1499
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1567
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1568..1654
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1821
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1822..1925
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1926..2053
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2054..6285
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2456..2461
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /note="hexamer: AATAAA"
     polyA_site      2483
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /note="major polyA site"
     regulatory      5692..5697
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /note="hexamer: AATAAA"
     polyA_site      5713
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /note="major polyA site"
     regulatory      6026..6031
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /note="hexamer: AATAAA"
     polyA_site      6052
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
     regulatory      6256..6261
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
                     /note="hexamer: AATAAA"
     polyA_site      6285
                     /gene="SLC5A12"
                     /gene_synonym="SMCT2"
ORIGIN      
        1 attccacccc cagtggttat taatttcaga acacatctga attccttctc tgtggcatat
       61 gctttaggag aggagcagac agctcttagc tagggtcaga tttcaaattc tcatctcttg
      121 gtgccaatac caccaccaga ttcttctttg aagtcaactt ttgagatctt cactaagtac
      181 acgttggtgt ctgaagattc acacgagtgc ctctggtaat cattttcttc agggaatcac
      241 agtctctcct ctcagcaaag catccactgt actgaacttt gcttttggaa acatcttctt
      301 cctgagacct cgttgaaaga aactctctgg tgtcatactt tccaatatgg aggtgaagaa
      361 ctttgcagtt tgggattatg ttgtatttgc agccctcttt ttcatttcct ctggaattgg
      421 ggtgttcttt gccattaagg agagaaaaaa ggcaacttcc cgagagttcc tggttggggg
      481 aaggcaaatg agctttggcc ctgtcggctt gtctctgaca gccagcttca tgtcagctgt
      541 cacggtcctg gggacccctt ctgaagtcta ccgctttggg gcatccttcc tagtcttctt
      601 cattgcttac ctatttgtca tcctcttaac atcagagctc tttctccctg tgttctacag
      661 atctggtatc accagcactt atgagtactt acaactacga ttcaacaaac cagttcgcta
      721 tgctgccacg gtcatctaca ttgtacagac gattctctac acaggagtgg tggtgtatgc
      781 tcctgccctg gcactcaatc aagtgactgg gtttgatctc tggggctctg tgtttgcaac
      841 aggaattgtt tgcacattct actgtaccct gggaggatta aaagcagtgg tgtggacaga
      901 tgcatttcag atggttgtca tgattgtggg cttcttaacg gttctcattc aaggatcaac
      961 tcatgctggg ggattccaca atgtattaga gcaatcaaca aatggatctc gactacatat
     1021 atttgacttt gatgtagatc ctctcaggcg acacactttt tggactatca cagtgggagg
     1081 aacttttact tggctcggaa tctatggggt caatcaatca actattcagc gatgcatctc
     1141 ttgcaaaaca gaaaagcatg ctaagcttgc cttgtatttt aacttgctgg gtctctggat
     1201 cattctggtg tgtgctgtct tctctggctt aatcatgtac tctcacttta aagactgtga
     1261 cccttggact tctggcatca tctcagcacc agaccagctg atgccgtact ttgtcatgga
     1321 gatatttgcc acaatgccag gactgccagg actttttgtg gcttgtgcct tcagtggaac
     1381 tctgagcacc gtggcttcca gcatcaatgc cttggcaaca gtgacctttg aggattttgt
     1441 caagagctgt tttcctcatc tctccgacaa gctgagcacc tggatcagta aaggcttatg
     1501 tctcttattt ggcgtgatgt gtacctctat ggctgtggct gcatctgtca tgggaggtgt
     1561 tgtgcaggct tccctcagca ttcacggcat gtgtggagga ccaatgctgg gcttattctc
     1621 cctgggaatc gtgttccctt ttgtgaattg gaagggtgca ctaggaggtc ttcttactgg
     1681 aatcaccttg tcattttggg tggccattgg ggccttcatt taccctgcac cagcctctaa
     1741 gacatggcct ttgcctctgt caacagacca atgtatcaaa tcaaatgtga cagcaacagg
     1801 gcctccagta ctatccagca gacctggaat agctgatacc tggtactcga tctcctacct
     1861 ttactacagt gcagtgggct gcttaggatg cattgttgct ggagtaatca tcagcctcat
     1921 aacaggtcgc caaagaggtg aggatattca accactgtta attagaccag tttgtaattt
     1981 attttgcttt tggtctaaga agtacaaaac actatgctgg tgtggagttc agcatgacag
     2041 tgggacagag caggaaaacc ttgagaatgg cagtgcccgg aaacaggggg ctgaatctgt
     2101 cttacagaac ggactcagaa gagaaagcct ggtacatgtt ccaggctatg atcctaagga
     2161 caaaagctac aacaatatgg catttgagac tacccatttc taaggcaata cctgtatgaa
     2221 tgcacacaca cacgtgcaat acacacacac acacacaaac tccacatact tcttgcctac
     2281 ttgttagtag atatgtatag ttgccattgc tagaagacag ggatgtctgg tgcctatttc
     2341 tacttattta taactacatg caaaatgact gtctctcggg atattctttg aaagactcca
     2401 actttcacag agaaaaacca acctgctcca aatgcccttg actacttcct tcttgaataa
     2461 attagggctg gatttcatta ccattcaaga aagcgaagtc tttttgcttg gtgtcatatt
     2521 aaacttcagg tttttcgttt tagtagtttt ttaaccatca aaatatcttg gagtttagag
     2581 gcagaacggg aaacagaaat atgcatattt aacactttcc tgccacgagg gataaaatag
     2641 aggaatgaca tccacccctg acctcatacc tgacatacat gtagatatat tttatgccac
     2701 ccatctccca tcctgtagct acaattggca tacaactact attaacctcc cttcaccacc
     2761 actgtcaggt cctcttccag tcattccagt cattagctgt cctgaccaaa cattaaaaaa
     2821 aaaattcagc taaatacaga agaagatggt atgtctggct agtgggagtg attataacta
     2881 aaaactttgc tccttttgtg ctgtccatgc agtatgtctt cttcctttct atcactttac
     2941 aatgaaaaat tgcctcagag ctcaataaga agtctagagc ctttttccag ggctaaggaa
     3001 agagaaaagg aatgtcctat agaaggttgt taggatagaa tttggtaaaa gaacgttgca
     3061 aatattgtaa cagaccatag gagatttcat cagcaatagg attcttcttt ggagaaaata
     3121 cattgtccat aagacttgta ctctgctcat tcaactcatg tgagcaagct caactcactc
     3181 cacctgggtt aggtaacaga agtggagaac ttcatagttc gtgtctagaa aataatgttt
     3241 aaagttctgg agaatgaggg tattgcagat taaaaggcga gttgacaaat gaaggagcag
     3301 tgaaagattt ttggaagaag tgaagaagtg aaattctgaa aaggtaaaag aaagaaccag
     3361 tatgtcacag gggccaagtc agaggacaga taataagaaa caaagttgta tctgagagtc
     3421 atatattagg acaggtgtca gatatttatt ttggtggcca gataaaagca aaaggcctag
     3481 aaacagtgtg ttagcaaagt aagaagaaat ggtccaaata ggcaaggata aggaaatcca
     3541 aaggttgtct ttaaatattt ctcaaaagag aaagccttga aagaagcata caatagagaa
     3601 aaaataaatt accagtattt attattagaa aagatagaaa gacagacaaa tcagtggagg
     3661 aattaaaaca gagaaactgg agtttataaa acagagccca atccttgcct tctctccctc
     3721 cactcaaata gaaaaggaga atggagaaag agaaagaagg tattaggcta cagtttataa
     3781 gagagatgag aaaaaaatac atttgggaat agagggaaag ggtcaaaagg ggtcacattt
     3841 ggagaaatat ctgaaaatga gaaggagcag aatttttgga aacatttttt aaagtctggc
     3901 aacgctaatt aagctgttga tctaaggatt tgcaaattga gaggtgcaat tattttccaa
     3961 atgatttgtg acactcttat taattagaat atatattttg tgaatattga aatctgagcc
     4021 aaaactagtt agctttatta atatcttagg gaaagaagag agaaagaaag agggagggag
     4081 agagagaaag aaagaaagaa agaaagaaag aaagaaaaga agaaagaaag aaagaaaaag
     4141 acaggaagag gaagagagag acagaaagaa taaaaaaaca gcaattctga ggatgagaat
     4201 aaattaatgg taggtattac atatgcatat acatatatat atctgtacac atatatatgt
     4261 aaatatgatt tggaatacat caggattgtg aacttccact tgggatagca taaaagcaaa
     4321 atataccata caatttttca ttttactcac aaattttact cacaaattct cctaaagaca
     4381 tttcactagg tcagtacatt tccgagaaag ttttaaaaac gtttcttctt ttatagtccc
     4441 taattatctt acctggagtg ttatcaaaat gtcatacaac tggaaaatgc aattattatt
     4501 ataaaaatat ctctgccttc tcttgtgtaa tgaaatttat ggcattagga gatgacattt
     4561 caagagattt tagaggtcaa tgtagagact ccccccattc ttcttaattg aacaaacatg
     4621 cataaatcta gggtttcagg tagagagcag atgggaatct acaggaaact gtcttccata
     4681 gtgtatgaaa accgtgtttg ttgtgtggtg aagcaaaagg aaactatgaa gcgcacagtc
     4741 aatagattca gctagtgagt cccagcactc ctttcaggcc ctcactagga ttagcagccc
     4801 agagtcatgc caaggccact ggtgcagtac aaagacagtt cagtgacaag ttccatccat
     4861 accatggaag agggttaaac ttgtggctgt ttttgccaca gcatgaccag agacattctc
     4921 aataaatggc actaggaatg atgagtttta gaggggccta aaatgttcca aacttgatac
     4981 tcaccactgt acctttaaag cacaaccaca gatattggta aaaatggaac agaattacct
     5041 caatacctct ttaactcatt ctgtatgaat tcattttgat gacagacatt ttttgtcacc
     5101 atttggaata agaactacaa cagcttatga ttgaggtgag gaactgcagt caaatggctt
     5161 aggttcaaac acgagttctg acacaaatct aggtaatttt gggcaatatt ttaaaatctt
     5221 agtttattca ccaattaata ggagtaaaaa ttacccctac ttaatagaat tattatgaag
     5281 atttatataa gtaaatcata taaagtgctt agcacagtgt gactggccca catggaacat
     5341 ttaatacttg gtctgcagta tatgaagaaa atggtgtaca tgttaactta aatggtttca
     5401 ttatttctct tcaggatttt taagatgtgg gatcagaata tatgttacta ttttgagaat
     5461 tagaaataat aagaaaaaca gaatgtatac attaaaagtc atgcctaaat ttccaaagag
     5521 tagttttttt aatgccagag tttaaataaa atattatttt ccagagagta acttttctaa
     5581 aggtcctaaa ttctgaaaag agagaaaact actgttataa acaagtttat tactaaaaga
     5641 tgcaattcat tactatgatt tattattatt ttgtacatgt aaaccttgat aaataaacag
     5701 catctcaacc caagtttatt actcattgca cagttcaaag caactatgac tctcaaaata
     5761 gcaacagaag gtggggaggg tcttttccct ttgtttatat agtatttgct aaaaatgaac
     5821 tttactattt tgtggcttta tagggttaca ccatttcatt ctaaagagaa acaaaaagta
     5881 aaccattggc catgtggctt tcaaacagta tttcaaagag aatattactg aaatattttt
     5941 gtgctgttac agtcttaatc aaacattatt acaaagatca gatacggaaa aaatagagga
     6001 gtctcccctt aattgttccc tttgaaataa attgttttaa attattacag aagttatagc
     6061 tgtatgtaca tgcacatttg tatatttaca agtctcatgt acttaatctc aatattgcaa
     6121 attagctagg tttactatag agttaactgc ttttatctag catttataat cttgtagtat
     6181 aacctatttt caaataacta aaatttaatt gatgtcatca atggtacaca tgaaaatgat
     6241 acaaatttat aaactaataa aatgtaactc gtcattgata gtaaa
//
