LOCUS       NM_001039960           11764 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens solute carrier family 4 member 8 (SLC4A8), transcript
            variant 1, mRNA.
ACCESSION   NM_001039960
VERSION     NM_001039960.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11764)
  AUTHORS   Wang,W., Tsirulnikov,K., Zhekova,H.R., Kayik,G., Khan,H.M.,
            Azimov,R., Abuladze,N., Kao,L., Newman,D., Noskov,S.Y., Zhou,Z.H.,
            Pushkin,A. and Kurtz,I.
  TITLE     Cryo-EM structure of the sodium-driven chloride/bicarbonate
            exchanger NDCBE
  JOURNAL   Nat Commun 12 (1), 5690 (2021)
   PUBMED   34584093
  REMARK    GeneRIF: Cryo-EM structure of the sodium-driven
            chloride/bicarbonate exchanger NDCBE.
            Erratum:[Nat Commun. 2021 Oct 13;12(1):6083. doi:
            10.1038/s41467-021-26412-7.. PMID: 34645810]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11764)
  AUTHORS   Sweeney,M.D., Zhao,Z., Montagne,A., Nelson,A.R. and Zlokovic,B.V.
  TITLE     Blood-Brain Barrier: From Physiology to Disease and Back
  JOURNAL   Physiol Rev 99 (1), 21-78 (2019)
   PUBMED   30280653
  REMARK    Review article
REFERENCE   3  (bases 1 to 11764)
  AUTHORS   Alvadia,C.M., Sommer,T., Bjerregaard-Andersen,K., Damkier,H.H.,
            Montrasio,M., Aalkjaer,C. and Morth,J.P.
  TITLE     The crystal structure of the regulatory domain of the human
            sodium-driven chloride/bicarbonate exchanger
  JOURNAL   Sci Rep 7 (1), 12131 (2017)
   PUBMED   28935959
  REMARK    GeneRIF: The crystal structure at 2.8 A resolution of the
            regulatory N-terminal domain of human NDCBE represents the first
            crystal structure of an electroneutral sodium-bicarbonate
            cotransporter. The crystal structure forms an equivalent dimeric
            interface as observed for the cytoplasmic domain of Band 3, and
            thus establishes that the consensus motif VTVLP is the key minimal
            dimerization motif.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 11764)
  AUTHORS   Sherva,R., Tripodis,Y., Bennett,D.A., Chibnik,L.B., Crane,P.K., de
            Jager,P.L., Farrer,L.A., Saykin,A.J., Shulman,J.M., Naj,A. and
            Green,R.C.
  CONSRTM   GENAROAD Consortium; Alzheimer's Disease Neuroimaging Initiative;
            Alzheimer's Disease Genetics Consortium
  TITLE     Genome-wide association study of the rate of cognitive decline in
            Alzheimer's disease
  JOURNAL   Alzheimers Dement 10 (1), 45-52 (2014)
   PUBMED   23535033
REFERENCE   5  (bases 1 to 11764)
  AUTHORS   Parker,M.D., Bouyer,P., Daly,C.M. and Boron,W.F.
  TITLE     Cloning and characterization of novel human SLC4A8 gene products
            encoding Na+-driven Cl-/HCO3(-) exchanger variants NDCBE-A, -C, and
            -D
  JOURNAL   Physiol Genomics 34 (3), 265-276 (2008)
   PUBMED   18577713
  REMARK    GeneRIF: This study confirmed the presence in human cDNA of mouse
            NDCBE-like transcripts (human NDCBE-A) and three novel transcripts
            NDCBE-C, NDCBE-D, and NDCBE-D'.
REFERENCE   6  (bases 1 to 11764)
  AUTHORS   Park,M., Ko,S.B., Choi,J.Y., Muallem,G., Thomas,P.J., Pushkin,A.,
            Lee,M.S., Kim,J.Y., Lee,M.G., Muallem,S. and Kurtz,I.
  TITLE     The cystic fibrosis transmembrane conductance regulator interacts
            with and regulates the activity of the HCO3- salvage transporter
            human Na+-HCO3- cotransport isoform 3
  JOURNAL   J Biol Chem 277 (52), 50503-50509 (2002)
   PUBMED   12403779
REFERENCE   7  (bases 1 to 11764)
  AUTHORS   Gresz,V., Kwon,T.H., Vorum,H., Zelles,T., Kurtz,I., Steward,M.C.,
            Aalkjaer,C. and Nielsen,S.
  TITLE     Immunolocalization of electroneutral Na(+)-HCO cotransporters in
            human and rat salivary glands
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 283 (2), G473-G480 (2002)
   PUBMED   12121896
REFERENCE   8  (bases 1 to 11764)
  AUTHORS   Soleimani,M.
  TITLE     Na+:HCO3- cotransporters (NBC): expression and regulation in the
            kidney
  JOURNAL   J Nephrol 15 Suppl 5, S32-S40 (2002)
   PUBMED   12027220
  REMARK    Review article
REFERENCE   9  (bases 1 to 11764)
  AUTHORS   Grichtchenko,I.I., Choi,I., Zhong,X., Bray-Ward,P., Russell,J.M.
            and Boron,W.F.
  TITLE     Cloning, characterization, and chromosomal mapping of a human
            electroneutral Na(+)-driven Cl-HCO3 exchanger
  JOURNAL   J Biol Chem 276 (11), 8358-8363 (2001)
   PUBMED   11133997
REFERENCE   10 (bases 1 to 11764)
  AUTHORS   Amlal,H., Burnham,C.E. and Soleimani,M.
  TITLE     Characterization of Na+/HCO-3 cotransporter isoform NBC-3
  JOURNAL   Am J Physiol 276 (6), F903-F913 (1999)
   PUBMED   10362779
  REMARK    Erratum:[Am J Physiol 1999 Sep;277(3 Pt 2):followi]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DQ063579.2, AC025097.41 and
            AW118662.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001039960.2.
            
            Summary: The protein encoded by this gene is a membrane protein
            that functions to transport sodium and bicarbonate ions across the
            cell membrane. The encoded protein is important for pH regulation
            in neurons. The activity of this protein can be inhibited by
            4,4'-Di-isothiocyanatostilbene-2,2'-disulfonic acid (DIDS). Several
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Apr 2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.143682.1,
                                           SRR1660809.108162.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000453097.7/ ENSP00000405812.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4211              DQ063579.2         11-4221
            4212-11688          AC025097.41        179773-187249       c
            11689-11764         AW118662.1         10-85               c
FEATURES             Location/Qualifiers
     source          1..11764
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.13"
     gene            1..11764
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /note="solute carrier family 4 member 8"
                     /db_xref="GeneID:9498"
                     /db_xref="HGNC:HGNC:11034"
                     /db_xref="MIM:605024"
     exon            1..205
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     CDS             158..3439
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /note="isoform a is encoded by transcript variant 1;
                     electroneutral sodium bicarbonate exchanger 1; k-NBC3;
                     electroneutral Na(+)-driven Cl-HCO3 exchanger; solute
                     carrier family 4, sodium bicarbonate cotransporter, member
                     8"
                     /codon_start=1
                     /product="electroneutral sodium bicarbonate exchanger 1
                     isoform a"
                     /protein_id="NP_001035049.1"
                     /db_xref="CCDS:CCDS44890.1"
                     /db_xref="GeneID:9498"
                     /db_xref="HGNC:HGNC:11034"
                     /db_xref="MIM:605024"
                     /translation="MPAAGSNEPDGVLSYQRPDEEAVVDQGGTSTILNIHYEKEELEG
                     HRTLYVGVRMPLGRQSHRHHRTHGQKHRRRGRGKGASQGEEGLEALAHDTPSQRVQFI
                     LGTEEDEEHVPHELFTELDEICMKEGEDAEWKETARWLKFEEDVEDGGERWSKPYVAT
                     LSLHSLFELRSCLINGTVLLDMHANSIEEISDLILDQQELSSDLNDSMRVKVREALLK
                     KHHHQNEKKRNNLIPIVRSFAEVGKKQSDPHLMDKHGQTVSPQSVPTTNLEVKNGVNC
                     EHSPVDLSKVDLHFMKKIPTGAEASNVLVGEVDILDRPIVAFVRLSPAVLLSGLTEVP
                     IPTRFLFILLGPVGKGQQYHEIGRSMATIMTDEIFHDVAYKAKERDDLLAGIDEFLDQ
                     VTVLPPGEWDPSIRIEPPKNVPSQEKRKMPGVPNGNVCHIEQEPHGGHSGPELQRTGR
                     LFGGLVLDIKRKAPWYWSDYRDALSLQCLASFLFLYCACMSPVITFGGLLGEATEGRI
                     SAIESLFGASMTGIAYSLFAGQALTILGSTGPVLVFEKILFKFCKDYALSYLSLRACI
                     GLWTAFLCIVLVATDASSLVCYITRFTEEAFASLICIIFIYEAIEKLIHLAETYPIHM
                     HSQLDHLSLYYCRCTLPENPNNHTLQYWKDHNIVTAEVHWANLTVSECQEMHGEFMGS
                     ACGHHGPYTPDVLFWSCILFFTTFILSSTLKTFKTSRYFPTRVRSMVSDFAVFLTIFT
                     MVIIDFLIGVPSPKLQVPSVFKPTRDDRGWIINPIGPNPWWTVIAAIIPALLCTILIF
                     MDQQITAVIINRKEHKLKKGCGYHLDLLMVAIMLGVCSIMGLPWFVAATVLSITHVNS
                     LKLESECSAPGEQPKFLGIREQRVTGLMIFVLMGCSVFMTAILKFIPMPVLYGVFLYM
                     GVSSLQGIQFFDRLKLFGMPAKHQPDFIYLRHVPLRKVHLFTLIQLTCLVLLWVIKAS
                     PAAIVFPMMVLALVFVRKVMDLCFSKRELSWLDDLMPESKKKKLDDAKKKAKEEEEAE
                     KMLEIGGDKFPLESRKLLSSPGKNISCRCDPSEINISDEMPKTTVWKALSMNSGNAKE
                     KSLFN"
     exon            206..287
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            288..434
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            435..570
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            571..731
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            732..920
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1012
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1170
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1258
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..1405
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1506
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1507..1681
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1682..1815
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1816..2061
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2062..2167
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2168..2329
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2330..2443
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2444..2605
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2606..2857
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2858..2926
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2927..3100
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            3101..3238
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            3239..3330
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            3331..3426
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            3427..11764
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5171..5176
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /note="hexamer: ATTAAA"
     polyA_site      5192
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
     regulatory      11743..11748
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /note="hexamer: AATAAA"
     polyA_site      11764
                     /gene="SLC4A8"
                     /gene_synonym="NBC3; NDCBE"
                     /note="major polyA site"
ORIGIN      
        1 gcgggccggt ggctatggag gcggcggcgg ttgatggttg accgttggct ccggggtggg
       61 ggtcgccgtt cgagtgatct gctcagaccc gaccagaggg cgcgggctgc tgatgcttgg
      121 cttggagccc gtgggggaga cctagttcgg ctccgccatg ccggccgccg ggagtaacga
      181 gccggacggc gtcctcagct atcagagacc agatgaagaa gctgtggtgg atcagggtgg
      241 gaccagtaca attctcaaca ttcactatga aaaagaagag ctggaaggtc acagaactct
      301 gtatgtggga gttcggatgc cgcttggccg gcagagccat cggcatcacc gcactcatgg
      361 ccagaagcac cggagacgag ggcggggcaa aggagccagc cagggggagg aaggcctgga
      421 agccctggcc cacgacacac catctcagcg tgttcagttc attcttggca ccgaggaaga
      481 tgaagagcat gtgcctcatg agctgtttac agagctggat gagatctgta tgaaagaggg
      541 agaagatgct gagtggaagg aaacagccag gtggctgaag tttgaagaag atgttgaaga
      601 tgggggagaa cgctggagca agccttatgt ggcaaccctt tcattgcaca gcctgtttga
      661 gctaaggagc tgccttatta atggaacagt cctcctggat atgcatgcaa atagcataga
      721 agaaatttca gacctgatcc tggatcagca agaactgtcc agtgacctga atgacagcat
      781 gagggttaaa gtgcgggaag cccttctcaa aaagcatcat catcagaatg aaaagaagag
      841 aaacaacctc attcccattg ttcgctcctt tgctgaggtt ggcaagaagc agtctgatcc
      901 tcatttgatg gataaacatg gtcaaaccgt gtctcctcag tctgttccaa ctacaaatct
      961 tgaagtaaaa aatggagtga attgtgaaca tagtcctgtg gatttaagca aggtagacct
     1021 tcatttcatg aaaaaaattc ctactggggc cgaggcctcc aatgtcctgg ttggagaggt
     1081 ggatattttg gaccgtccca ttgttgcctt tgtgaggctg tctccagctg ttcttctctc
     1141 aggcctaaca gaagtgccaa tcccaacaag atttttgttt atcttattgg gtccagtagg
     1201 gaaaggtcag cagtaccatg agattggcag atccatggcc accatcatga cagatgagat
     1261 ttttcatgac gtagcatata aggcaaaaga gcgagatgat ctcctggcgg ggattgatga
     1321 gttcctagac caggtgacgg tgctccctcc aggagagtgg gatccctcca ttagaattga
     1381 gccacccaaa aatgtccctt cccaggagaa aaggaaaatg cctggagttc caaatggaaa
     1441 tgtttgccac atagaacagg aaccacatgg gggtcacagt gggccagaac ttcagcgcac
     1501 tgggcggcta tttgggggct tggtgctgga catcaagcgg aaggccccct ggtactggag
     1561 cgactaccga gatgcactca gcttacagtg tttggcttcc tttctgttcc tgtactgtgc
     1621 ctgcatgtca cctgtcatca cctttggggg actgcttgga gaagccactg agggacgcat
     1681 aagtgcaatt gaatccttgt ttggagcttc catgactggg attgcttatt ccttgtttgc
     1741 gggacaggct ctcaccatcc tgggaagtac tggaccagtg cttgtgtttg aaaagatttt
     1801 gttcaaattc tgcaaagact atgctctttc atacctctcc ctgcgagctt gtattggact
     1861 gtggaccgct ttcctgtgta ttgtccttgt ggcaactgat gccagttccc ttgtctgcta
     1921 cattacccgt ttcactgaag aagcatttgc ctccctaatt tgcattattt tcatctatga
     1981 agcaatagaa aaactgattc acctggcaga gacctacccc atccacatgc acagccagct
     2041 ggaccacctt agcctctatt actgcaggtg tactctgcca gagaatccaa acaatcacac
     2101 cctccagtac tggaaggacc acaacatcgt gacagcagaa gtccactggg ctaacctgac
     2161 tgtcagtgaa tgccaggaga tgcatggaga gttcatggga tctgcgtgcg gccatcatgg
     2221 accctacact cctgatgtcc tcttttggtc ctgtattctc tttttcacca ccttcatcct
     2281 ctcaagcacc ttaaagacgt ttaagacgag ccgttatttc ccaaccagag tacgctccat
     2341 ggtgagtgac tttgctgttt tcctcactat cttcacaatg gtgattattg attttttgat
     2401 tggagtccca tcaccaaagc ttcaagttcc cagtgtgttc aagccaacaa gggatgatcg
     2461 cggatggatt attaatccca ttggccccaa tccctggtgg actgtgatag ctgcaattat
     2521 cccagctctt ctctgtacta tcttgatatt catggatcag cagatcacag ccgtcattat
     2581 taacaggaag gaacataagc tcaagaaagg ctgtggctac cacctggacc tactgatggt
     2641 ggccatcatg ctgggtgtct gctccatcat gggcctgccc tggtttgtag ctgcaactgt
     2701 cttgtccatc acacatgtga acagcctcaa gctagaatct gaatgctctg ctcctggaga
     2761 acagcccaag ttcctgggca tccgagaaca gagagtgaca ggccttatga tctttgtgct
     2821 gatgggctgc tcagtcttca tgacggctat cttaaagttt attccaatgc cagtactcta
     2881 cggagttttc ctttacatgg gagtttcttc actacaggga attcagttct ttgatcgtct
     2941 aaagctcttt gggatgcccg caaagcacca gccagatttc atctacctgc ggcatgtgcc
     3001 gctgcgcaaa gtgcacctct tcaccctcat ccagttgacc tgtctcgtcc tgctctgggt
     3061 catcaaggca tctccagctg ccattgtttt cccaatgatg gttttggcct tggtctttgt
     3121 caggaaagtc atggatctct gtttctctaa gcgagagctg agctggctag atgatctcat
     3181 gcctgaaagc aaaaagaaga agttggatga tgccaaaaag aaggccaagg aggaagagga
     3241 ggctgagaaa atgttagaaa ttgggggaga caagtttccc ttagagagca ggaagttact
     3301 aagtagtcct ggaaagaaca tcagttgcag atgtgacccc tctgagatta atatatctga
     3361 tgaaatgcct aaaactacag tttggaaagc tctcagtatg aattctggaa atgcaaagga
     3421 aaagagtctc ttcaactaag agtctttgct gggatggaag atttgggccg tgtggtgcct
     3481 cagggaagtt ctggttacag agaaaatggc gagtctctca gagaagaagc aagaccaagt
     3541 ctggccctgt ccttggtcat ctcaaagcca tgccgaagca ttcagttatt cttggtgtgc
     3601 attggaaggc atccagctat ccccatacca gcagccagtc accagatgtg aatgtggaag
     3661 cagaagacca cctcctgttg gttcttctcc tcttccttct ttttctcttt agaacggcca
     3721 ccattgaaga cctagcttcc cattttccag acgttttctc tgaaattctc tgctggcctg
     3781 ccaagccata tggattcatt ctgccactga ggagtccttc agtgaggtcc ctcttcctaa
     3841 aggacagagt ggggagtagg aggggaacag agaggacatc ctctctggct ctccagtgct
     3901 cttagtgtct acaggctcct aggcagccct gggccttggt ttgattacct cccctggggg
     3961 atgctggtca gacccagagg ttgtcaggag gtcagctacc aggaagatcc atgatctggg
     4021 cattggcagt gcctgccacc acagccagga agatgcctct gacctgggtg catctccatc
     4081 actccttagc agcagcctgc ataactggca agaatcttgg atgatacaag agccaagaag
     4141 ggacatttga gttgtgtcgc ttagatagga aagggatcca gggaaaatca acagtaagtg
     4201 aggatgagca gtgtctcttg gttttcattg aggatagagt aagagattga gtttagattg
     4261 caacagaagg aattagttta gataccagga agaacttcct agcctgaaga tttgtcatag
     4321 tgtctgcttt ctagatatct gggaaagatt tgataatagt tgtttgtgaa tagaaaggag
     4381 gatatgatgt ttttattggc cattttgcgg gactcttcga cttcttgctg ctgtctcttg
     4441 aggatacatt ccaattccat cctggcgaga tccaagtgct tacgtactgt ctccttagct
     4501 gccttagagt aaacgatcat cagttcaatg gaccaaaatc accttcagcc atgtggtttc
     4561 ttcatcatca tggatttctt ttggttgaca aacattctgg ctctcagatg caaaaagtca
     4621 cactgggaaa tgaactgtaa gtggtgaaat tagttttggt atttaattta aaactacatt
     4681 tatagttttt ctcttctctt ctatgttgca atgaatgtaa agtatttggg atccagtgct
     4741 tataaacctt tccttccttt gtgcacagaa tgtaactagc aagcccatta gcacccagat
     4801 aattctatca tgttagtttc ccatcctgga aaatctttgt acagtgggaa gttccccgat
     4861 gtgtttttct ttcttaggtg aagggttggc tatatcactt tattgaattt tgcattcctt
     4921 agacttttaa aatatactaa tgtattctag tcttactcta aagacctttg atgttaaagg
     4981 aatccttcat ttatttcata ttccctatct catagggcca caattatttt aatacagaga
     5041 tgattttcaa aatattttaa caactggtac aggacagatg ccagccactc agaagggatg
     5101 cctgctgtaa acaagcagta tgtatggttg taccaatgcc tattggctga acattatgct
     5161 actttcagat attaaaatgg tgttcctttg aatcgtgggt attgttcctg gtttgtctca
     5221 tttctatcac aaggctgttt tgggcacagc cttagcttcc tttcatatat atatggtgca
     5281 gacaggatag gtcatgcttc ttggaaccta tatgggcatt aaaatcttca tgagccaaag
     5341 cttcacattt tagcacttta ggataactgt agtaattgta ctcatttacc tacctaattg
     5401 ggaagaatta aaaggcagcc catctggagg ataagctttc ttgcagactt ttcccttgtc
     5461 cctctctggc tgcctccttg agcttgccac tttttcatct tcattgttcc ctgagaacat
     5521 ctcccctttc ttgctctatc ttgcccctcc catcttttcc tgccacctct gtgtggggag
     5581 gttgatgacc atttcacctc tttccctagc ctcagcacag ctgtaaggag tgtatcaaaa
     5641 ctgggtgaga gtatcactca gaaaaatgcc tcccctaaca cacggatgtc ttgtgctgcc
     5701 ttgaccgctt gaggggagga gccttcatta actctgtgta gtgcaggtac aggaccacct
     5761 gtgcacttta gggtacttct cgagctagac cctcctggat cactgcgcct taggaatgtt
     5821 agctactttt taaaaaaaat atgatttggt gcgttgttgg ggtgaactcc atggggaaga
     5881 ttgtgagtga agatgggaag gaatttgggt ggttttgcca cgaggtgatg gttggggttg
     5941 ggcgaatggg ttggatgatc ctggctctgt gggtgtggtg ctcggttgcc tccgtacagt
     6001 tgcagttcac ctttgtgcat ggtgattacc agtcactcta gggattgtag gtcccaacag
     6061 cggttccagc agcctgcaca gctggtacga ggacagctgg aggagggtct gatatccctg
     6121 caagtgatgc aggataaaac ttcccggccg ggcgcaatgg ctcgcgcctg taatcccagc
     6181 actttgggag gccaagatgg gcggatcacg aggtcaggag atcgagacca tcctggctaa
     6241 cgtggtgaaa ccccgtctct actaaaaata caaaaaaatt agctgggcat ggtggcgggc
     6301 gcctgtagtc ccagctattc aggaggctga ggcagaatgg cgtgaacgcg ggaggcagag
     6361 cttgcagtga gccgagatca tgccactgca ctccagcctg ggtgacagag caagactcca
     6421 tttcaaaaaa aaaaaaaaaa aaaaacttcc caataggagg tattccttga tggctctcga
     6481 atgcatgaat gatatatcaa tttgaagttt ctcgaccttt ccatcaagga tcttgaattt
     6541 tttctcaatt atgcttgcta atgtgtcaaa ggccaagtac actgtatcaa acttgccgtc
     6601 tgtaagatgc aagatcaatc tagtgtttca gccagttcac tactgacatc agactcaact
     6661 ttgtcccctc tctttctttt catgcaagag actttaaggg taacctcttc agttgaaact
     6721 gtcttggaag tgcagccctc ctcctgtgtg gctagagaaa gagatggcac caggatctct
     6781 taacggcaag tgctcacggg gcatgggtga ttttgtctag tctggtcctt ttggacgtgg
     6841 tgatttcctg tcatcctcgt ggtctgacat tgcttctcac tggatactgg ttgtggcctt
     6901 tgactcatta gctgattgtt ggatctcttt gtgaggttcg attttttaaa aatccatggg
     6961 tccctatgga ctgtcacctg ttgcagatga tggtgattct cttctttttt ttgtctccaa
     7021 tagttgcctg gacgacttca tgggtcagtc cacatgattg caggatttcc cattgttcat
     7081 ctgtgaatgt tgattggcca acctgtctga gctttcagca gatgcatcct tgagcttact
     7141 ttcaggaatt cctcctggaa ttggttttcc ttggtggtaa tttctcatgg tggagcttgt
     7201 ttctgaggca tggtacagtg ctgcggaggc aaggcaaggc tgtcatggtg attttcacag
     7261 catctggatg gcaaattgcc ttcacgtagg gaaaccacat gggactgata gctttctcaa
     7321 gggatctcca agcatctctg aatttgggga tttcatcagt tactcctgga atcaaaatga
     7381 gtgtggagtc ataatatata ttttttttga gacaaagtct cactctgtcg cccaggctgg
     7441 agtgcagtgg tgcgatctca tctcactgca acctctgcct cctgggttca agtgattctc
     7501 ctgcctcagc ctcccaagta gctaggatta caggtgcgca ccaccatgcc cagctaattt
     7561 ttgtatttta gtagagacga agtttcacta tgttggccag gctggtctga aactcctggc
     7621 ctcaggtgat ccacccacct cagcctccca aagtgctggg attacaggta tgagccactg
     7681 cccctggcct caaaatcttt aaggaagtta tttatcgtca caagtgttgt catctgtgac
     7741 tcatgggcca cttgatgaac ctgaaccatt agtggttgtc cagatcctcc aactttttca
     7801 ggcagtattc caatcagggt tttacgacac cagggtatcc cacctttttt gcaacatagg
     7861 cagaaacacc aagctgtggg taattgatgt gcgtgtcact gattcctgca acagctttga
     7921 atgtattaaa tctgttttca acagcccttg cataggtgtt atctctcaaa tctctgtaaa
     7981 gagcactaag agtatgtagt gttgcatgat ataatccaag cctgtgggct gtgtcatccg
     8041 ggattgttgg aattttgcat gtgtctgttc catatcccgc tggtaaccag cttacagacc
     8101 ttctgtcagg ttggcttaca ttatcatcaa caggttgccg tagtgatgat atgatgctgg
     8161 acacaatcat tgtgcacttt gttaatttgg tatggtggtg ccacacatta tcactgtaca
     8221 gcattaacaa ggctggaata tggccctctg ctgtaggggg cgtggcacct gtgacttgca
     8281 ctggaataag taggaggctt tccccaaagg atatggagac tgatgtgtgc tccatatctt
     8341 gaggggagct ctcttggttt cctttggccg ggtctgttag ttacttttga aggccatgcc
     8401 aaccctttta ggccattaaa tttttctcaa acaatagcca gcctcatttc tgcttcatct
     8461 ctgtggtagg aaccattccc tactcttact ccctcctctt gtccaacttc caccaaacac
     8521 tcaatccgag tcctggctgc tttcttgctt aggtctgtgg ctggtcactg tggtgaaaga
     8581 gtcaaggtgg gcagtgtttg gggtaggaaa tgagtagtgg tgaagcagca gcagtgtgtg
     8641 tgtgggtgtg ggtgtgtgtg catgcgtgtg catttgcatg tgtgtatgtt gacaaatgag
     8701 agtgtagcca taagatttca ttgttactac tagtctttgt gtacctatct ggagggacat
     8761 taaaaaaata tggccattct cccatctagg gggttcccat ctcagacttc tctgataaga
     8821 gtaatttgag aacaaataca gattcatcta tgggaaaaga tgtttaaact cagcatgaag
     8881 gagaaacaaa tttaggagtg gaaagcaagg caggtagtta ggtgatttcc atgaccacat
     8941 ttgcttgggc cagggaagtg gtgcacctct tttataacaa tgggagattt cagaaacagt
     9001 ttgagtgttg gcagaccaat gccctgataa gcttgtggta tagaggtagg aagggaggga
     9061 ggccagagat tagataaaat gatggtgggt gggatcttag ctctaaatga aaagaactgg
     9121 acttactaag tgggtggatt acttgctagc cataactttt agggaagggt aggtctggag
     9181 agaaggtgaa gaccagcaga aatatactgt gaaattgcca aatatgtttg gttgcagaaa
     9241 acacagcctg gctctttgtg gctagatgtg acttccaact tctgaagaca ggattgccaa
     9301 gagatgtgat ctttccacat acagctcctg tccccatctc tttatgtgta gagacctgga
     9361 tttggggtag tggggtagta aaccatatat ttccaaaaga tggtgtgaag agtcgtttct
     9421 cttcgtctgg gatgtgattg gcttttgttt tttgtttttt gtttttgaga cggagtctcg
     9481 ctctgtcgct ttgtcaccag gctggagtgc aatggcacaa tctcagctca ctgcaacctc
     9541 cgcctcctgg cttcaagtga ttctcctgcc tcagcctcct gagtagctgg gactataggc
     9601 gcgcaccacc atgcccagct aatttttgta tttttagtag agatgggatt tcaccatgtt
     9661 ggccaggatg gtcttgatct cttgacctca tgatccaccc gccttggcct cccaaagtgc
     9721 tgggattaca ggcttgagct actgcgcccg gccagtgatt ggcttttaaa ttaatcacaa
     9781 atgtttgata aaattctggt atgtgctaag tcccaggctt ggttgatatc tcagactatg
     9841 atagctgtga ccttcaagaa atttctggct tggggaatct gtagtttctg gttgtccaaa
     9901 aaagatagtt cttagtccta ctttattttc tacccactca actctccaga cttccctctt
     9961 agtaaaggaa ttcataattc tccctgcatc ttctctgttt atttcaatgt ccatgttctg
    10021 agtctcaagt tttcctgaag cacaggagca ggcttggccc cagagcccct ggctttttca
    10081 acgagcatca gaaatgctat caatatattc tctctgttgc tttatcagct tctctaaatt
    10141 tattttgtaa ggaagttagc acctccttcc aggactttaa acagttgtct ttgccaattt
    10201 gttcctggat tttccttgaa cttctcaggt ttccaagcca catcctagca gggcatccag
    10261 gagccttgca ctgaacctct cagctctttt gactttcttc tggtcatagg tgttgggcct
    10321 cccattaggt agaagtcctt tgagcagacc cgaaatggcc aaatgagaca tcatccaagt
    10381 tcctccctcc tttactgtct cggctttttc aagcacccct ttcacctctc ttttctgcct
    10441 tttcctcagt ctgtcaagtt ctttggagga aagaagttct tggtcagagg tcctaaaacc
    10501 accaccagct gggggtgctg agaatggtga ggagttggac agtcccgggg cttttttgaa
    10561 aggggacttt atggtcattt cccctgttta gggtgaggga ctaagaattc tcaagccttc
    10621 agtttcatcc atatttcaat gtaagcagaa aagcacatct caaagccaaa tagaaatgat
    10681 tttctactaa gcctatcctt tgtgattctt ggttcccttg gtctcttaat attaattata
    10741 gagaatgggc agttgagtca gttaacatct tttcatcaga aaggagggta atattcataa
    10801 ccaaaagaga tgtaaaggaa gtatattact gcttgaagtg tgaaaagagg aaaggagtgt
    10861 tatgtgaacc ttttcagtag ggaaattcag aaaatggaat gattcatcca taggcataat
    10921 tcttttagga gattctgtgc tcaaagggaa gggaatggtt tctgatcctt ctgaagagaa
    10981 aaggaatagc atttttctta aacctaaccc agtttcagca ttggagaata cagaactttt
    11041 tcttctagct gatggcatta atatttcttg agagagagaa ctcacccatg gcacttttct
    11101 gagcccagca gaaatcagcg gagcttgggc ttcgcttagc aggtttgcaa ttgacttcaa
    11161 catgcaggct tttcacatgt gcaataatgc tggaaacaga agcaccaaac tgattgtgca
    11221 attactcctt ttgtagaaga ggccaaaatc ctcctcctcc ttcctttctc ctatattcac
    11281 tcctccagga tcataaagcc tccctcttgt ttatctgtgt ctgtctgtct gattggttag
    11341 atttggctgc ccttccaagc taatggtgtc aggtggagaa cagagcaacc ttccctcgga
    11401 aggagacaat tcgaggtgct ggtacatttc ccttgttttc tatgttcttc tttctagtgg
    11461 gtctcatgta gagatagaga tatttttttg ttttagagat tccaaagtat atatttttag
    11521 tgtaagaaat gtaccctctc cacactccat gatgtaaata gaaccaggaa taaatgtgtc
    11581 attgtgataa tcccatagca atttatggta agaacaagac ccctttccct caccaccgag
    11641 tctcgtggtc tgtgtctgtg aaccagggca ggtaattgtg acactgcatc tcatagaact
    11701 ctgcctgccc agatttttgt gtgctcacct caatgggtga aaaataaagt ctgtgtaaac
    11761 tgtt
//
