LOCUS       NM_001321103            8079 bp    mRNA    linear   PRI 23-APR-2024
DEFINITION  Homo sapiens solute carrier family 4 member 7 (SLC4A7), transcript
            variant 4, mRNA.
ACCESSION   NM_001321103 XM_011534255
VERSION     NM_001321103.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Axelsen,T.V., Olesen,C., Khan,D., Mohammadi,A., Bouzinova,E.V.,
            Nielsen,C.J.F., Mele,M., Hauerslev,K.R., Pedersen,H.L., Balling,E.,
            Vahl,P., Tramm,T., Christiansen,P.M. and Boedtkjer,E.
  TITLE     Antibodies toward Na+,HCO3--cotransporter NBCn1/SLC4A7 block net
            acid extrusion and cause pH-dependent growth inhibition and
            apoptosis in breast cancer
  JOURNAL   Br J Cancer 130 (7), 1206-1220 (2024)
   PUBMED   38310186
  REMARK    GeneRIF: Antibodies toward Na[+],HCO3[-]-cotransporter NBCn1/SLC4A7
            block net acid extrusion and cause pH-dependent growth inhibition
            and apoptosis in breast cancer.
REFERENCE   2  (bases 1 to 8079)
  AUTHORS   Severin,M., Pedersen,E.L., Borre,M.T., Axholm,I.,
            Christiansen,F.B., Ponniah,M., Czaplinska,D., Larsen,T., Pardo,L.A.
            and Pedersen,S.F.
  TITLE     Dynamic localization of the Na+-HCO3- co-transporter NBCn1 to the
            plasma membrane, centrosomes, spindle and primary cilia
  JOURNAL   J Cell Sci 136 (7) (2023)
   PUBMED   37039101
  REMARK    GeneRIF: Dynamic localization of the Na+-HCO3- co-transporter NBCn1
            to the plasma membrane, centrosomes, spindle and primary cilia.
REFERENCE   3  (bases 1 to 8079)
  AUTHORS   Zhou,Y., Ma,X. and Sun,J.
  TITLE     Update on the relationship between the SLC4A7 variant rs4973768 and
            breast cancer risk: a systematic review and meta-analysis
  JOURNAL   J Int Med Res 51 (4), 3000605231166517 (2023)
   PUBMED   37128157
  REMARK    GeneRIF: Update on the relationship between the SLC4A7 variant
            rs4973768 and breast cancer risk: a systematic review and
            meta-analysis.
REFERENCE   4  (bases 1 to 8079)
  AUTHORS   Ali,E.S., Liponska,A., O'Hara,B.P., Amici,D.R., Torno,M.D., Gao,P.,
            Asara,J.M., Yap,M.F., Mendillo,M.L. and Ben-Sahra,I.
  TITLE     The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de
            novo nucleotide synthesis
  JOURNAL   Mol Cell 82 (17), 3284-3298 (2022)
   PUBMED   35772404
  REMARK    GeneRIF: The mTORC1-SLC4A7 axis stimulates bicarbonate import to
            enhance de novo nucleotide synthesis.
REFERENCE   5  (bases 1 to 8079)
  AUTHORS   Pushkin,A., Abuladze,N., Newman,D., Muronets,V., Sassani,P.,
            Tatishchev,S. and Kurtz,I.
  TITLE     The COOH termini of NBC3 and the 56-kDa H+-ATPase subunit are PDZ
            motifs involved in their interaction
  JOURNAL   Am J Physiol Cell Physiol 284 (3), C667-C673 (2003)
   PUBMED   12444018
REFERENCE   6  (bases 1 to 8079)
  AUTHORS   Park,M., Ko,S.B., Choi,J.Y., Muallem,G., Thomas,P.J., Pushkin,A.,
            Lee,M.S., Kim,J.Y., Lee,M.G., Muallem,S. and Kurtz,I.
  TITLE     The cystic fibrosis transmembrane conductance regulator interacts
            with and regulates the activity of the HCO3- salvage transporter
            human Na+-HCO3- cotransport isoform 3
  JOURNAL   J Biol Chem 277 (52), 50503-50509 (2002)
   PUBMED   12403779
  REMARK    GeneRIF: The cystic fibrosis transmembrane conductance regulator
            interacts with and regulates the activity of the bicarbonate
            salvage trransporter isoform 3
REFERENCE   7  (bases 1 to 8079)
  AUTHORS   Pushkin,A., Yip,K.P., Clark,I., Abuladze,N., Kwon,T.H.,
            Tsuruoka,S., Schwartz,G.J., Nielsen,S. and Kurtz,I.
  TITLE     NBC3 expression in rabbit collecting duct: colocalization with
            vacuolar H+-ATPase
  JOURNAL   Am J Physiol 277 (6), F974-F981 (1999)
   PUBMED   10600945
REFERENCE   8  (bases 1 to 8079)
  AUTHORS   Pushkin,A., Abuladze,N., Lee,I., Newman,D., Hwang,J. and Kurtz,I.
  TITLE     Cloning, tissue distribution, genomic organization, and functional
            characterization of NBC3, a new member of the sodium bicarbonate
            cotransporter family
  JOURNAL   J Biol Chem 274 (23), 16569-16575 (1999)
   PUBMED   10347222
REFERENCE   9  (bases 1 to 8079)
  AUTHORS   Pushkin,A., Abuladze,N., Lee,I., Newman,D., Hwang,J. and Kurtz,I.
  TITLE     Mapping of the human NBC3 (SLC4A7) gene to chromosome 3p22
  JOURNAL   Genomics 57 (2), 321-322 (1999)
   PUBMED   10198178
REFERENCE   10 (bases 1 to 8079)
  AUTHORS   Ishibashi,K., Sasaki,S. and Marumo,F.
  TITLE     Molecular cloning of a new sodium bicarbonate cotransporter cDNA
            from human retina
  JOURNAL   Biochem Biophys Res Commun 246 (2), 535-538 (1998)
   PUBMED   9610397
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK309734.1, EU934248.1,
            EU934249.1, FJ178574.1, EU499349.1, AK310865.1, AF053755.1,
            AF047033.1, AL389947.1 and AA569234.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001321103.1.
            
            Summary: This locus encodes a sodium bicarbonate cotransporter. The
            encoded transmembrane protein appears to transport sodium and
            bicarbonate ions in a 1:1 ratio, and is thus considered an
            electroneutral cotransporter. The encoded protein likely plays a
            critical role in regulation of intracellular pH involved in visual
            and auditory sensory transmission. Alternatively spliced transcript
            variants encoding distinct isoforms have been described. [provided
            by RefSeq, Apr 2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU934248.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000454389.6/ ENSP00000390394.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                AK309734.1         37-73
            38-494              EU934248.1         1-457
            495-997             EU934249.1         446-948
            998-1130            EU934248.1         961-1093
            1131-1408           FJ178574.1         1043-1320
            1409-2603           EU934249.1         988-2182
            2604-2604           EU499349.1         2183-2183
            2605-3773           EU934249.1         2184-3352
            3774-3774           AK310865.1         132-132
            3775-4196           EU934248.1         3738-4159
            4197-5412           AF053755.1         3181-4396
            5413-6279           AF047033.1         5092-5958
            6280-8078           AL389947.1         668-2466
            8079-8079           AA569234.1         11-11               c
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p24.1"
     gene            1..8079
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /note="solute carrier family 4 member 7"
                     /db_xref="GeneID:9497"
                     /db_xref="HGNC:HGNC:11033"
                     /db_xref="MIM:603353"
     exon            1..318
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     CDS             259..4038
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /note="isoform d is encoded by transcript variant 4;
                     sodium bicarbonate cotransporter 2; sodium bicarbonate
                     cotransporter 3; electroneutral Na/HCO(3) cotransporter;
                     bicarbonate transporter; sodium bicarbonate cotransporter
                     2b; solute carrier family 4, sodium bicarbonate
                     cotransporter, member 7"
                     /codon_start=1
                     /product="sodium bicarbonate cotransporter 3 isoform d"
                     /protein_id="NP_001308032.1"
                     /db_xref="CCDS:CCDS82747.1"
                     /db_xref="GeneID:9497"
                     /db_xref="HGNC:HGNC:11033"
                     /db_xref="MIM:603353"
                     /translation="MEADGAGEQMRPLLTRVTSRGPDEEAVVDLGKTSSTVNTKFEKE
                     ELESHRAVYIGVHVPFSKESRRRHRHRGHKHHHRRRKDKESDKEDGRESPSYDTPSQR
                     VQFILGTEDDDEEHIPHDLFTEMDELCYRDGEEYEWKETARWLKFEEDVEDGGDRWSK
                     PYVATLSLHSLFELRSCILNGTVMLDMRASTLDEIADMVLDNMIASGQLDESIRENVR
                     EALLKRHHHQNEKRFTSRIPLVRSFADIGKKHSDPHLLERNGEGLSASRHSLRTGLSA
                     SNLSLRGESPLSLLLGHLLPSSRAGTPAGSRCTTPVPTPQNSPPSSPSISRLTSRSSQ
                     ESQRQAPELLVSPASDDIPTVVIHPPEEDLEAALKGEEQKNEENVDLTPGILASPQSA
                     PGNLDNSKSGEIKGNGSGGSRENSTVDFSKVDMNFMRKIPTGAEASNVLVGEVDFLER
                     PIIAFVRLAPAVLLTGLTEVPVPTRFLFLLLGPAGKAPQYHEIGRSIATLMTDEIFHD
                     VAYKAKDRNDLLSGIDEFLDQVTVLPPGEWDPSIRIEPPKSVPSQEKRKIPVFHNGST
                     PTLGETPKEAAHHAGPELQRTGRLFGGLILDIKRKAPFFLSDFKDALSLQCLASILFL
                     YCACMSPVITFGGLLGEATEGRISAIESLFGASLTGIAYSLFAGQPLTILGSTGPVLV
                     FEKILYKFCRDYQLSYLSLRTSIGLWTSFLCIVLVATDASSLVCYITRFTEEAFAALI
                     CIIFIYEALEKLFDLGETYAFNMHNNLDKLTSYSCVCTEPPNPSNETLAQWKKDNITA
                     HNISWRNLTVSECKKLRGVFLGSACGHHGPYIPDVLFWCVILFFTTFFLSSFLKQFKT
                     KRYFPTKVRSTISDFAVFLTIVIMVTIDYLVGVPSPKLHVPEKFEPTHPERGWIISPL
                     GDNPWWTLLIAAIPALLCTILIFMDQQITAVIINRKEHKLKKGAGYHLDLLMVGVMLG
                     VCSVMGLPWFVAATVLSISHVNSLKVESECSAPGEQPKFLGIREQRVTGLMIFILMGL
                     SVFMTSVLKFIPMPVLYGVFLYMGVSSLKGIQLFDRIKLFGMPAKHQPDLIYLRYVPL
                     WKVHIFTVIQLTCLVLLWVIKVSAAAVVFPMMVLALVFVRKLMDLCFTKRELSWLDDL
                     MPESKKKKEDDKKKKEKEEAERMLQDDDDTVHLPFEGGSLLQIPVKALKYSVDPSIVN
                     ISDEMAKTAQWKALSMNTENAKVTRSNMSPDKPVSVKISFEDEPRKKYVDAETSL"
     exon            319..400
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            401..547
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            548..686
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            687..847
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            848..1036
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1408
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            1409..1524
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..1682
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..1770
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..1917
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2024
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            2025..2199
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            2200..2333
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            2334..2579
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            2580..2685
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            2686..2847
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            2848..2961
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            2962..3123
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            3124..3375
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            3376..3444
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            3445..3618
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            3619..3750
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            3751..3848
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            3849..3956
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     exon            3957..8079
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7285..7290
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /note="hexamer: ATTAAA"
     polyA_site      7308
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /note="major polyA site"
     regulatory      8060..8065
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /note="hexamer: AATAAA"
     polyA_site      8079
                     /gene="SLC4A7"
                     /gene_synonym="NBC2; NBC3; NBCN1; SBC2; SLC4A6"
                     /note="major polyA site"
ORIGIN      
        1 agttctagct tcaggttcca cgccgcggcc tcgctctcct actaaagcca gcccagcagt
       61 cgcagcgcgc acacgcacgc cccgcgtctc ccgcgcccgg cgcccggctc tccccacgcg
      121 cgcacacctc gcgcacggac gcacccacgt ttgtcctcgc gcgccggggg ctcggcggcg
      181 gcggcagggg cagcggcaga agcaggcaga ccgcgcgggg cagtaccgta gcggccgtca
      241 cgggctggcc ggccggccat ggaggctgat ggggccggcg agcagatgag accgctactc
      301 acccgggtaa cgagcagggg tcctgatgaa gaagctgttg tggatcttgg caaaactagc
      361 tcaactgtga acaccaagtt tgaaaaagaa gaactagaaa gtcatagagc tgtatatatt
      421 ggtgttcacg tcccgtttag taaagagagt cgtcggcgtc ataggcatcg cggacacaaa
      481 catcaccacc ggagaagaaa agataaagaa tcagataaag aagatggacg ggaatctcct
      541 tcttatgata caccatccca gagagttcag tttatccttg gtactgaaga tgatgatgaa
      601 gaacatattc cccatgatct cttcacggaa atggatgaac tgtgttacag agatggagaa
      661 gaatatgaat ggaaagaaac tgctagatgg ctgaaatttg aagaggatgt tgaagatggc
      721 ggtgaccgat ggagtaaacc ttatgtggca actctctctt tgcacagtct ttttgaacta
      781 aggagttgca tcctcaatgg aacagtcatg ctggatatga gagcaagcac tctagatgaa
      841 atagcagata tggtattaga caacatgata gcttctggcc aattagacga gtccatacga
      901 gagaatgtca gagaagctct tctgaagaga catcatcatc agaatgagaa aagattcacc
      961 agtcggattc ctcttgttcg atcttttgca gatataggca agaaacattc tgaccctcac
     1021 ttgcttgaaa ggaatgggga aggcctttca gcctcccgcc actctttgcg aacaggtctg
     1081 tctgcctcaa acctttcctt gagaggagaa tcacctttat ctcttcttct tggtcatctt
     1141 cttccttctt caagagctgg aacccctgca ggctcaaggt gtacaacccc agtacccacc
     1201 cctcaaaaca gtcctccttc tagccctagc atcagccgcc tgacctccag aagttcccaa
     1261 gagagtcagc gtcaggcccc agaactactg gtttcacctg ccagtgatga tattcccaca
     1321 gtagtaattc atccgcctga ggaagactta gaagcagcgc tgaaaggcga ggagcagaag
     1381 aatgaggaaa atgttgactt aactccaggt attttggcct ctccccagtc tgctcctgga
     1441 aacttggaca atagtaaaag tggagaaatt aaaggtaatg gaagtggtgg aagcagagaa
     1501 aatagtactg ttgacttcag caaggttgat atgaatttca tgagaaaaat tcctacgggt
     1561 gctgaggcat ccaacgtcct ggtgggcgaa gtagactttt tggaaaggcc aataattgca
     1621 tttgtgagac tggctcctgc tgtcctcctt acagggttga ctgaggtccc tgttccaacc
     1681 aggtttttgt ttttgttatt gggtccagcg ggcaaggcac cacagtacca tgaaattgga
     1741 cgatcaatag ccactctcat gacagatgag attttccatg atgtagctta taaagcaaaa
     1801 gacagaaatg acctcttatc tggaattgat gaatttttag atcaagtaac tgtcctacct
     1861 ccaggagagt gggatccttc tatacgcata gaaccaccaa aaagtgtccc ttctcaggaa
     1921 aagagaaaga ttcctgtgtt tcacaatgga tctaccccca cactgggtga gactcctaaa
     1981 gaggccgctc atcatgctgg gcctgagcta cagaggactg gacggctttt tggtggtttg
     2041 atacttgaca tcaaaaggaa agcacctttt ttcttgagtg acttcaagga tgcattaagc
     2101 ctgcagtgcc tggcctcgat tcttttccta tactgtgcct gtatgtctcc tgtaatcact
     2161 tttggagggc tgcttggaga agctacagaa ggcagaataa gtgcaataga gtctcttttt
     2221 ggagcatcat taactgggat tgcctattca ttgtttgctg ggcaacctct aacaatattg
     2281 gggagcacag gtccagttct agtgtttgaa aaaattttat ataaattctg cagagattat
     2341 caactttctt atctgtcttt aagaaccagt attggtctgt ggacttcttt tttgtgcatt
     2401 gttttggttg caacagatgc aagcagcctt gtgtgttata ttactcgatt tacagaagag
     2461 gcttttgcag cccttatttg catcatattc atctacgagg ctttggagaa gctctttgat
     2521 ttaggagaaa catatgcatt taatatgcac aacaacttag ataaactgac cagctactca
     2581 tgtgtatgta ctgaacctcc aaaccccagc aatgaaactc tagcacaatg gaagaaagat
     2641 aatataacag cacacaatat ttcctggaga aatcttactg tttctgaatg taaaaaactt
     2701 cgtggtgtat tcttggggtc agcttgtggt catcatggac cttatattcc agatgtgctc
     2761 ttttggtgtg tcatcttgtt tttcacaaca ttttttctgt cttcattcct caagcaattt
     2821 aagaccaagc gttactttcc taccaaggtg cgatcgacaa tcagtgattt tgctgtattt
     2881 ctcacaatag taataatggt tacaattgac taccttgtag gagttccatc tcctaaactt
     2941 catgttcctg aaaaatttga gcctactcat ccagagagag ggtggatcat aagcccactg
     3001 ggagataatc cttggtggac cttattaata gctgctattc ctgctttgct ttgtaccatt
     3061 ctcatcttta tggatcaaca aatcacagct gtaattataa acagaaagga acacaaattg
     3121 aagaaaggag ctggctatca ccttgatttg ctcatggttg gcgttatgtt gggagtttgc
     3181 tctgtcatgg gacttccatg gtttgtggct gcaacagtgt tgtcaataag tcatgtcaac
     3241 agcttaaaag ttgaatctga atgttctgct ccaggggaac aacccaagtt tttgggaatt
     3301 cgtgaacagc gggttacagg gctaatgatt tttattctaa tgggcctctc tgtgttcatg
     3361 acttcagtcc taaagtttat tccaatgcct gttctgtatg gtgttttcct ttatatggga
     3421 gtttcctcat taaaaggaat ccagttattt gaccgtataa aattatttgg aatgcctgct
     3481 aagcatcagc ctgatttgat atacctccgt tatgtgccgc tctggaaggt ccatattttc
     3541 acagtcattc agcttacttg tttggtcctt ttatgggtga taaaagtttc agctgctgca
     3601 gtggtttttc ccatgatggt tcttgcatta gtgtttgtgc gcaaactcat ggacctgtgt
     3661 ttcacgaaga gagaacttag ttggcttgat gatcttatgc cagaaagtaa gaaaaagaaa
     3721 gaagatgaca aaaagaaaaa agagaaagag gaagctgaac ggatgcttca agatgatgat
     3781 gatactgtgc accttccatt tgaaggggga agtctcttgc aaattccagt caaggcccta
     3841 aaatatagtg ttgatccctc aattgttaac atatcagatg aaatggccaa aactgcacag
     3901 tggaaggcac tttccatgaa tactgagaat gccaaagtaa ccagatctaa catgagtcct
     3961 gataaacctg tgagtgtgaa aataagtttt gaagatgaac caagaaagaa atacgtggat
     4021 gctgaaactt cattatagaa ttgaaccaag aggcattata catatagata tatacatatg
     4081 taatgtgtgc gtatcatgtc actatatata agaatattgt atgtcatgct gtttatgtgt
     4141 gactaccggg tttttaaaag tagtgtctgg agtttgtaat gagcaccgtg gagactatgt
     4201 atttaatgaa atgctctctt tgaagtgagg tacatggttc ttaactattc aaatatttat
     4261 tctgttagaa aaaaaaattt tctgttttgc aatagaagga tgtggagaaa tgctttcagt
     4321 ctacttttct taaatctctg ttcatcagtg gcaattcgta aaaaccttaa gtgatacttt
     4381 gtttatatgt ttataatttt taggtgtttc ctgaaatttt cacatattat ttcacttttg
     4441 ttagtgcttt atgggaagaa tagggagtct ataccagtgc tgtgggaaaa atggtaacat
     4501 ttcagggctt ctctatttgt gtttcatttc tgtagatgtc catcgtgttt cactaactgg
     4561 cgttttctta gccatagaga tgactgtaga acaatagaaa ctttaataat gatagttttt
     4621 aacttttatg tttaaatttt ttttaaatct taaaaccttc atatctaggt atccattgtg
     4681 acagacaagt aaaattgcag gtgatttgat aattaagcac ccataccatt tataacttct
     4741 gaatttaaaa agttatacaa tgccagtttg caatagttga ttttgatgcc tttgtagaat
     4801 attttttctg aatccttatg ctcttttaaa gccaatgatt cccactctgt tctctgcctt
     4861 gtctctcttt gtcttaaaat gctttagttt ccatcaggtt caagttcttg actattattc
     4921 tcttataagt agtaggcgta aataatcagg agttagaatt ctctcagaag ggtctatgat
     4981 cagtattact ttattaagaa ttacctttca ttttctcttt atgttattct ttcacttttg
     5041 tagattacat ttaatagctt gttacctgtg attttatttt aaaatattta ttttgatatg
     5101 atgcttaaat attatataaa catttggaaa agtaacaaat agagtaaatt gttaatgtaa
     5161 atagttggtg cttactttca tttatgttta ttatcttaaa gcaattgata gattttacat
     5221 ctttgatata aagcactgcc atatttatat tttaaaagga aattagacat tttatatgta
     5281 gctcagatta atgacatttt tattttgtgt attagttttt gcttttctga gctttttaaa
     5341 gtctaacaaa tctttttagt catcttttat atacttttag ttccacatga aataaatgtt
     5401 gttaagcctg taggactgga tgaatggggt tgtgaaactg atttgacaag agaataattt
     5461 acaaaaatca aatgagtatt gagaagccac agaaatatca aaaatggtga catcactatt
     5521 aggatgaaaa ttttatgaaa ttccaatgct ccctttaacc atataattaa attacaaagg
     5581 tatacataat taatctaata aaggatcatg aggaatgcca aaagctgaaa ttttagcaag
     5641 tctggtgttt taaattcatt ataaactatt tattaactaa tgtaatgtcc tcctctaaga
     5701 ccatattgta agagtcttgt taaaatgatg gattcaactt ctgcctcgga tgagaggttg
     5761 gaaatgtagc tgcttttctt tagaaaatat gtagctgtca tcatttggta ctgcctaaaa
     5821 agagttagac tctattggca attgatagag tattacccac ggtgcttatt attgtttatg
     5881 agcttccatt gtaatgattc ctttttattt gtagcagcat aattatttcc aaagacccca
     5941 gtatttgctg ctatttttaa aactcccctg atgtacctga acaagaggat ttcctcacat
     6001 catttccttg tgtctggaca tcaggggtaa caactgtact tactttacag gaagaaattt
     6061 taaaactgaa aactacctgg gatcatagtg tttctgtgat tttatttaat tgtgtatagt
     6121 aattatccag tgccagaaaa accgtcactt gcaaaatact ttgcactcaa aatgttttta
     6181 caatgcttct aaatgttact ggtttctgct ttcttttgac tacttgactg acaaaatgat
     6241 ctgactactc catttaagag caaaggtaac tcatgtttag gtaattaact gcttgtttta
     6301 agtgattata tttcttccac tgtttttgaa aaataatcaa agatagcatt cattgagaga
     6361 cagtgacaga tataatttac tacatatatt gatttttaaa taaagttgcc ttaaataagt
     6421 gtatgtaagc agtagtagtt gctatgtact gatttacctc aaggtgcaaa ataattaaac
     6481 ctgtacatat tccatttaca aaataaattc agccctgcac tttctttaga tgccttgatt
     6541 tccagaatgg agcttagtgc tactgaatac cctggccaca gagccacctc aggatattct
     6601 tttctccacc ctagtttatt tatttataga tatctgttta caaagtctgt agtaaatcct
     6661 gatgctgacc atctgaaatg tacttttttt ctgaatgctg tttcaatcta aaatagcagc
     6721 ttttgagaaa acaatgatgt aaattcctta tgataaaagg atgattctat atattcttta
     6781 atgatattaa atatgccgaa gccaagcaca cagtctttct aaagtgtgtg tatgtttgtg
     6841 tgaatgtgaa tgatactgat cttatatctg ttaaaagttg ttttaaaaag ctgtggcatc
     6901 ccattgttca tatttgccaa gtcttctgta aagatgtcta ggacgaaata ttttatgtgc
     6961 taatgcatgt atttgtaaac cagatttgtt taccactcaa aattaacttg ttttcttcat
     7021 ccaaaaaagt ttatttcttc cacgtactta aattttctgt gtgggtataa tatagctttc
     7081 taattttttt ctttcacaaa ggcaggttca aaattctgtt gaaagaaaaa tgctttctga
     7141 aactgaggta taacaccaga gcttgctgtt taaaggatta tatgatgtac atcagttcta
     7201 taaatgtgct cagcagttta acatgtgaat cctgttttaa agtgctcaga tttcaactgt
     7261 gtaagccatt gatataacgc tgtaattaaa aatgtttata tgaaataact taatgtttta
     7321 aatttattta tgtagatcac atcattttta tcgtatgcag tgcaaatatg tgaaatgtct
     7381 tttggtttat tccaacaatt atttatttta gaaagtaagt ttaaagactt taaggacatt
     7441 caaagtttaa aatagtgttc aaattgcaaa atttggcaat cttcatataa attggtttct
     7501 tttctaactt ttcaaaaact aacattaaat gtcaattata ggaaaacata gttggaaatg
     7561 taatcatcca aagatcattt ttaaaatgaa atttaattag cacatattga acatttgact
     7621 taattgttaa accccagttt tgttttgttt ttttaatcag atttttgcac actgattagt
     7681 ttttgtgttg tggcttttgt tgctttatta ttcaaggttt tttttttttt tcttccccat
     7741 gggggagatt gtcttccaat gtttaactac gtttaaataa ataaaaattg aattttattg
     7801 ttcatttata taaaatctga taccttgatg taatttcaca atacagttcc aatttttatg
     7861 gcttttataa ttacaatgat attttcttct ataataaaat ccaaagtaaa catttaaatt
     7921 gtagaactga tatttttcat ttatatgaag tataagcctc tactgggtct atattgtgaa
     7981 tcatcctgcc tttcaaattt gtttcataat tgttagatga aaactatttt tttggagatg
     8041 ttactgaagt tgattgagca ataaaagtct acttaatta
//
