LOCUS       NM_133478               6357 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens solute carrier family 4 member 5 (SLC4A5), transcript
            variant c, mRNA.
ACCESSION   NM_133478 XM_005264465
VERSION     NM_133478.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Gildea,J.J., Xu,P., Kemp,B.A., Carlson,J.M., Tran,H.T., Bigler
            Wang,D., Langouet-Astrie,C.J., McGrath,H.E., Carey,R.M., Jose,P.A.
            and Felder,R.A.
  TITLE     Sodium bicarbonate cotransporter NBCe2 gene variants increase
            sodium and bicarbonate transport in human renal proximal tubule
            cells
  JOURNAL   PLoS One 13 (4), e0189464 (2018)
   PUBMED   29642240
  REMARK    GeneRIF: NBCe2 activity is stimulated by an increase in
            intracellular sodium and is hyper-responsive in hRPTCs carrying HV
            SLC4A5 rs7571842 through an aberrant HNF4A-mediated mechanism
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Gildea,J.J., Xu,P., Carlson,J.M., Gaglione,R.T., Bigler Wang,D.,
            Kemp,B.A., Reyes,C.M., McGrath,H.E., Carey,R.M., Jose,P.A. and
            Felder,R.A.
  TITLE     The sodium-bicarbonate cotransporter NBCe2 (slc4a5) expressed in
            human renal proximal tubules shows increased apical expression
            under high-salt conditions
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 309 (11), R1447-R1459 (2015)
   PUBMED   26447209
  REMARK    GeneRIF: NBCe2 could be important in proximal kidney tubule apical
            sodium and bicarbonate cotransport under high-salt conditions.
REFERENCE   3  (bases 1 to 6357)
  AUTHORS   Wen,D. and Sansom,S.C.
  TITLE     Physiological role of NBCe2 in the regulation of electrolyte
            transport in the distal nephron
  JOURNAL   Am J Physiol Renal Physiol 309 (6), F489-F491 (2015)
   PUBMED   26136555
  REMARK    GeneRIF: we summarize the potential function of NBCe2 in the
            regulation of blood pressure, acid-base, and K(+) and Ca(2+)
            transport in the distal nephron--{REVIEW}
            Review article
REFERENCE   4  (bases 1 to 6357)
  AUTHORS   Xie,P., Kranzler,H.R., Yang,C., Zhao,H., Farrer,L.A. and
            Gelernter,J.
  TITLE     Genome-wide association study identifies new susceptibility loci
            for posttraumatic stress disorder
  JOURNAL   Biol Psychiatry 74 (9), 656-663 (2013)
   PUBMED   23726511
REFERENCE   5  (bases 1 to 6357)
  AUTHORS   Carey,R.M., Schoeffel,C.D., Gildea,J.J., Jones,J.E., McGrath,H.E.,
            Gordon,L.N., Park,M.J., Sobota,R.S., Underwood,P.C., Williams,J.,
            Sun,B., Raby,B., Lasky-Su,J., Hopkins,P.N., Adler,G.K.,
            Williams,S.M., Jose,P.A. and Felder,R.A.
  TITLE     Salt sensitivity of blood pressure is associated with polymorphisms
            in the sodium-bicarbonate cotransporter
  JOURNAL   Hypertension 60 (5), 1359-1366 (2012)
   PUBMED   22987918
  REMARK    GeneRIF: Data indicate that 2 SNPs in SLC4A5 (rs7571842 and
            rs10177833) showed highly significant results and large effects
            sizes.
REFERENCE   6  (bases 1 to 6357)
  AUTHORS   Virkki,L.V., Wilson,D.A., Vaughan-Jones,R.D. and Boron,W.F.
  TITLE     Functional characterization of human NBC4 as an electrogenic
            Na+-HCO cotransporter (NBCe2)
  JOURNAL   Am J Physiol Cell Physiol 282 (6), C1278-C1289 (2002)
   PUBMED   11997242
REFERENCE   7  (bases 1 to 6357)
  AUTHORS   Sassani,P., Pushkin,A., Gross,E., Gomer,A., Abuladze,N.,
            Dukkipati,R., Carpenito,G. and Kurtz,I.
  TITLE     Functional characterization of NBC4: a new electrogenic
            sodium-bicarbonate cotransporter
  JOURNAL   Am J Physiol Cell Physiol 282 (2), C408-C416 (2002)
   PUBMED   11788353
REFERENCE   8  (bases 1 to 6357)
  AUTHORS   Soleimani,M. and Burnham,C.E.
  TITLE     Na+:HCO(3-) cotransporters (NBC): cloning and characterization
  JOURNAL   J Membr Biol 183 (2), 71-84 (2001)
   PUBMED   11562789
  REMARK    Review article
REFERENCE   9  (bases 1 to 6357)
  AUTHORS   Pushkin,A., Abuladze,N., Newman,D., Lee,I., Xu,G. and Kurtz,I.
  TITLE     Cloning, characterization and chromosomal assignment of NBC4, a new
            member of the sodium bicarbonate cotransporter family
  JOURNAL   Biochim Biophys Acta 1493 (1-2), 215-218 (2000)
   PUBMED   10978526
REFERENCE   10 (bases 1 to 6357)
  AUTHORS   Pushkin,A., Abuladze,N., Newman,D., Lee,I., Xu,G. and Kurtz,I.
  TITLE     Two C-terminal variants of NBC4, a new member of the sodium
            bicarbonate cotransporter family: cloning, characterization, and
            localization
  JOURNAL   IUBMB Life 50 (1), 13-19 (2000)
   PUBMED   11087115
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC006030.2, DB455137.2 and
            AF293337.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_133478.2.
            
            Summary: This gene encodes a member of the sodium bicarbonate
            cotransporter (NBC) family, part of the bicarbonate transporter
            superfamily. Sodium bicarbonate cotransporters are involved in
            intracellular pH regulation and electroneural or electrogenic
            sodium bicarbonate transport. This protein is thought to be an
            integral membrane protein. Multiple transcript variants encoding
            different isoforms have been found for this gene, but the
            biological validity of some variants has not been determined.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (c) lacks an internal exon in the
            coding region as compared to variant a. Variant c encodes a protein
            that lacks a 16 aa internal segment.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000394019.7/ ENSP00000377587.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 AC006030.2         727-735
            10-60               DB455137.2         8-58
            61-6357             AF293337.2         1-6297
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p13.1"
     gene            1..6357
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /note="solute carrier family 4 member 5"
                     /db_xref="GeneID:57835"
                     /db_xref="HGNC:HGNC:18168"
                     /db_xref="MIM:606757"
     exon            1..61
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            62..138
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            139..187
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            188..338
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            339..405
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            406..486
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     CDS             408..3773
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /note="isoform c is encoded by transcript variant c;
                     electrogenic sodium bicarbonate cotransporter 4; solute
                     carrier family 4 (sodium bicarbonate cotransporter),
                     member 5"
                     /codon_start=1
                     /product="electrogenic sodium bicarbonate cotransporter 4
                     isoform c"
                     /protein_id="NP_597812.1"
                     /db_xref="CCDS:CCDS1937.1"
                     /db_xref="GeneID:57835"
                     /db_xref="HGNC:HGNC:18168"
                     /db_xref="MIM:606757"
                     /translation="MKVKEEKAGVGKLDHTNHRRRFPDQKECPPIHIGLPVPTYPQRK
                     TDQKGHLSGLQKVHWGLRPDQPQQELTGPGSGASSQDSSMDLISRTRSPAAEQLQDIL
                     GEEDEAPNPTLFTEMDTLQHDGDQMEWKESARWIKFEEKVEEGGERWSKPHVSTLSLH
                     SLFELRTCLQTGTVLLDLDSGSLPQIIDDVIEKQIEDGLLRPELRERVSYVLLRRHRH
                     QTKKPIHRSLADIGKSVSTTNRSPARSPGAGPSLHHSTEDLRMRQSANYGRLCHAQSR
                     SMNDISLTPNTDQRKNKFMKKIPKDSEASNVLVGEVDFLDQPFIAFVRLIQSAMLGGV
                     TEVPVPTRFLFILLGPSGRAKSYNEIGRAIATLMVDDLFSDVAYKARNREDLIAGIDE
                     FLDEVIVLPPGEWDPNIRIEPPKKVPSADKRKSVFSLAELGQMNGSVGGGGGAPGGGN
                     GGGGGGGSGGGAGSGGAGGTSSGDDGEMPAMHEIGEELIWTGRFFGGLCLDIKRKLPW
                     FPSDFYDGFHIQSISAILFIYLGCITNAITFGGLLGDATDNYQGVMESFLGTAMAGSL
                     FCLFSGQPLIILSSTGPILIFEKLLFDFSKGNGLDYMEFRLWIGLHSAVQCLILVATD
                     ASFIIKYITRFTEEGFSTLISFIFIYDAIKKMIGAFKYYPINMDFKPNFITTYKCECV
                     APDTVNTTVFNASAPLAPDTNASLYNLLNLTALDWSLLSKKECLSYGGRLLGNSCKFI
                     PDLALMSFILFFGTYSMTLTLKKFKFSRYFPTKVRALVADFSIVFSILMFCGIDACFG
                     LETPKLHVPSVIKPTRPDRGWFVAPFGKNPWWVYPASILPALLVTILIFMDQQITAVI
                     VNRKENKLKKAAGYHLDLFWVGILMALCSFMGLPWYVAATVISIAHIDSLKMETETSA
                     PGEQPQFLGVREQRVTGIIVFILTGISVFLAPILKCIPLPVLYGVFLYMGVASLNGIQ
                     FWERCKLFLMPAKHQPDHAFLRHVPLRRIHLFTLVQILCLAVLWILKSTVAAIIFPVM
                     ILGLIIVRRLLDFIFSQHDLAWIDNILPEKEKKETDKKRKRKKGAHEDCDEEPQFPPP
                     SVIKIPMESVQSDPQNGIHCIARKRSSSWSYSL"
     exon            487..678
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            679..808
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            809..969
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1122
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1218
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1274
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1432
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1520
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1675
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            1676..1885
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..2060
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            2061..2194
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            2195..2466
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            2467..2525
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            2526..2726
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            2727..2840
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            2841..3002
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            3003..3181
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            3182..3254
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            3255..3323
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            3324..3497
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            3498..3653
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            3654..3738
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            3739..3806
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     exon            3807..6357
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6333..6338
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /note="hexamer: AATAAA"
     polyA_site      6357
                     /gene="SLC4A5"
                     /gene_synonym="NBC4; NBCe2"
                     /note="major polyA site"
ORIGIN      
        1 gcaaggtcca ccctaaagga attggagtca gggcccagga gccacaggcc actgcactgg
       61 ggcatcccag gatttggaat tcagctgtga gctcatgagc tgatggatgt ttccaggagt
      121 accctgaaga ccacccaggt gggagtgtaa gataaccttc cagataaaga gttgctgcta
      181 ccaaaagagt gctgacacca tcacagtatg ttcccttctc aaccctggac atggttcaga
      241 aacagctaaa gcagacaaga atctggggca gccaatattg taccacctgc caaagcttgg
      301 cacaaacaat ctggaagcca aaaattgcac atactgtgga acagagcaag tggttctggc
      361 ctccctggga tttggatcct ggtttccaga gctaagaaca caaagtcatg aaggtgaagg
      421 aggagaaggc tggggtagga aagctggacc acactaacca caggaggaga tttccggatc
      481 agaaagaatg ccctcctatc cacattgggc ttccagtacc cacttaccct caaagaaaaa
      541 ctgaccagaa gggacatctt tcaggcctgc aaaaagtcca ctggggcctg cggccagacc
      601 agccacagca ggaactgact ggcccaggga gtggggcaag cagccaggac agcagcatgg
      661 atcttatcag caggactcgg tccccagctg ctgagcagct ccaggacatc ctgggggagg
      721 aagatgaggc tcccaacccc accctcttta cagagatgga tactctgcag catgacggag
      781 accagatgga gtggaaggag tcagccaggt ggataaagtt tgaagaaaag gtagaggaag
      841 gcggcgaacg ctggagcaag ccccacgtgt ccacactatc cctgcacagc ctcttcgagc
      901 tccgtacctg cctgcagacg gggacggtgc tgctggattt ggacagtggc tccttaccac
      961 agatcataga tgatgtcatt gagaagcaga ttgaggatgg tctcctgcgg ccagagctcc
     1021 gggagagggt cagttacgtc ctcctgagga ggcaccgcca ccaaaccaag aagcccatcc
     1081 accgctcctt agctgacatt gggaagtcag tctccaccac aaatcgcagt cctgcccgga
     1141 gccctggtgc tggcccgagt ctacaccact ccacggaaga cctgcggatg cggcagagtg
     1201 caaattacgg acgtctgtgt catgcccaga gcagaagcat gaatgacatt tctctcaccc
     1261 caaacacaga ccagcggaaa aacaaattca tgaagaagat ccccaaggac tcagaagcgt
     1321 ccaacgtgct cgtgggcgag gtggacttcc tagaccagcc attcatcgcg ttcgtgcgcc
     1381 tcatccagtc ggccatgctg ggaggagtga ccgaggtgcc tgtccccacc agatttctgt
     1441 ttatactact gggaccttct gggagagcaa aatcctacaa tgaaattggc cgtgccattg
     1501 caaccctcat ggtagatgat ctcttcagtg acgtggccta caaagcccgc aatcgggaag
     1561 atctgatcgc aggaattgat gaatttctgg atgaggtcat cgtccttcct cctggagaat
     1621 gggacccaaa tatccggatt gagcccccca agaaggtgcc ctctgctgac aagaggaaat
     1681 ctgtgttctc cctagcagag ctgggccaga tgaatggctc tgtgggagga ggcggcggag
     1741 ctcctggagg aggcaatgga ggtggtggtg gtggtggcag tggcggcggg gctggcagtg
     1801 gcggggccgg cggaacaagc agcggggatg atggagagat gccagccatg catgaaatcg
     1861 gggaggaact tatctggaca ggaaggttct tcggtggcct gtgtctggat atcaagagga
     1921 agttgccctg gttcccaagt gacttctatg atggcttcca cattcagtcc atctctgcca
     1981 tcctattcat ctacctcggc tgtatcacca acgcgatcac ctttggtggg cttctggggg
     2041 atgccaccga caattatcag ggagtgatgg agagcttcct gggcactgcc atggctggct
     2101 ccttgttctg cctcttctcg ggacagcctc tcatcattct cagcagcacg gggcccatcc
     2161 tcatctttga gaagctcctc ttcgacttca gcaaaggcaa tggcctggac tacatggagt
     2221 tccgcctctg gattggccta cactcagctg tccagtgcct tatcctagtg gccacagatg
     2281 ccagctttat catcaaatat atcacccgct tcaccgagga gggcttctcc acccttatca
     2341 gcttcatctt catctacgat gccatcaaga agatgatcgg tgccttcaag tactacccta
     2401 tcaatatgga cttcaagcca aacttcatca ctacctacaa gtgcgagtgt gtcgcccctg
     2461 acacagtgaa tacaaccgtg ttcaatgctt cagccccatt ggcaccagac accaacgctt
     2521 ctctgtacaa cctccttaac ctcacagcgt tggactggtc cctgctgagc aagaaggagt
     2581 gtctgagcta cggcgggcgc ctgcttggga attcctgcaa gtttatccca gacctggcgc
     2641 tcatgtcctt catccttttc tttgggacat actccatgac cctgaccctg aagaagttca
     2701 aattcagccg ctattttcct accaaggtcc gggccctggt ggctgacttt tccattgttt
     2761 tctccatcct gatgttctgt ggaatcgatg cctgttttgg cctagaaact cccaagctgc
     2821 atgtgcccag tgtcatcaag ccaacgcggc ctgaccgagg ctggttcgtg gccccctttg
     2881 ggaagaaccc gtggtgggta tacccagcaa gcatcctgcc cgccctgctg gtgaccatcc
     2941 tgatcttcat ggaccagcag atcactgccg tcattgtcaa ccggaaggag aacaaactga
     3001 agaaggctgc cggctaccat ctggacctgt tctgggtggg catcctcatg gctttgtgct
     3061 cctttatggg gctcccctgg tacgtggctg ccacggtcat ctccatcgcc cacatcgaca
     3121 gcctcaagat ggagacagag accagtgccc ctggggagca gccccagttt ctgggagtca
     3181 gggaacagag agtaaccggc atcatcgtct tcatcctgac gggaatctct gtcttcctgg
     3241 ctcccatcct aaagtgtatc cccctgccgg tgctgtacgg agtcttcctc tacatgggcg
     3301 tggcctccct gaatggcatc cagttctggg aacgctgcaa gctcttcctg atgccagcca
     3361 agcaccagcc ggaccatgcc ttcctgcggc acgtgccgct gcgccggatc cacctcttca
     3421 ccctggtgca gatcctctgc ctggcggtgc tctggatcct caaatccacg gtggctgcca
     3481 tcatcttccc ggtcatgatc ctgggcctca tcatcgttcg aaggcttctg gatttcatct
     3541 tttcccagca cgacctggcc tggattgaca acatcctccc agagaaggaa aaaaaggaga
     3601 cagacaagaa gaggaagaga aaaaaagggg cccacgagga ctgtgatgag gagccccagt
     3661 tccctcctcc ctcggttata aagattccca tggaaagtgt ccaatcagat ccccaaaacg
     3721 gtatccactg cattgccaga aaaagatctt ccagttggag ttactcactc tgattcttcc
     3781 ttcagtgaca cagaacttga ccgaagcatc accttgcact tcaaaatatc ctgtccagct
     3841 tcacctgctt tcagctactc acggaaccca gtgttcatgg tgccacaggt gaagatagag
     3901 atggagtcag actatgactt cacagacatg gataaatacc gaagagaaac tgacagtgag
     3961 accaccctct aggatgaagc agcttctccc agatgggaga tggagagcct ggcttagtct
     4021 tcactgggag ggtcttaact tctctgtgcc tgtttccttc tttatcaaac agagggagtc
     4081 cgtctgccta tagaaaactg gagatctatt gatgaaaagt attgcttaga tgcaaagctc
     4141 tcccctttgt agtaattaaa atacctaaag ctaaatatta tattttattt taaaatgcag
     4201 ttcagcatag aaattctaac atttctttgg aaataaaaaa atgcttgttt ccccaaacca
     4261 tagctaagat aaaggggcaa tagtcaattc atatttaaaa caaatatcaa tttaaaattg
     4321 tttaaaggtt agatttaaat atagatgttc tcaaacaaac ttgtaatata cttttatgtc
     4381 actagaccat ctttggaata tttggcaatt ctgcatatta agaaatgctg aaaaggtcct
     4441 atcttttctc cctgtcacat ccaatttcct aagggttata tgaaaagttt gaaccagccg
     4501 ggcacagtgg cacacgcctg taatcctagc actttgggag gccgaggcag gtgaattgcc
     4561 tgagctcagg agttcgagac cagcctaggc aacacggtga aaccccgtct ctactaaact
     4621 acaaaaaatt agccgggtgt ggtggcatgc acctgtagtc ccagctattc gggaggctga
     4681 ggcaggagaa tcgcttgaac ccaggaggca gaagttgcag tgagctgaga tggcaccact
     4741 gcactccagc ctaggcaaca gagtgagact ccgtatccaa acaaaaaaaa agtttgaacc
     4801 atctgcatta tgggccttgc tccagaagtg tgactggggc tttcccagat tgaaaagcca
     4861 gttccaaaac tctgttaatg aaagaaccaa tgcatctgag tggataaatg tttctaaaat
     4921 aaaatagttc ttgttaaagt gaaatttgaa accacatgtt aaatcaaaat gatcctttaa
     4981 attagtatca ctggtttttc atacctggaa tcacatttca tgatacattt gggtgggtcc
     5041 tccctgcaaa ttttcaatgt ttattcccaa agtcaaactc ccattcatgt gaacaataca
     5101 ggttagacag aggtaaggtc acattttaca ttacaacttt gcaatattaa tcaacctagt
     5161 actttaaagg aatgattgaa aactatttgg gaagataaaa accagtttca atgaagcaaa
     5221 cctattcctc atgaaactta agatcagtca ttttatcttc aatccacatt taatgaacac
     5281 caaccatgtt gatttaatgc ctactatgac cctgacagcc acagtgcatc tgacacttac
     5341 tttctccaaa acaactatga tttaaacctt gagtaaatca cttgaacctt gaggttagag
     5401 ttactatatt tgctcaagat gacaaatgca ggtgctttct tttttaaaga aaaatgacac
     5461 tagatcatta acaccttgag tataggacag gtaattgcct ctgtaattcc cagcgtctag
     5521 tgtagtgcct ggtccataga aagctctcaa taagtgtttg attacattta atctcagagc
     5581 tcccttccaa gggatactgc caagaggtaa agctccaaac ctctactctt actgagagca
     5641 gaatttctta gaatttccag aaccatctcc tatgcttaag tataaattct tgtcagacac
     5701 agtggctcac acctgtaatc ccgctacttg ggaggcagag gtggggggaa tcagcagagg
     5761 tcaggaggtc aagaccagcc tgggcaacat agtgagaccc atctctaaac aaatgtttaa
     5821 aaattagcta ggcatgctgg cacacacctg tagtcccagc catttcagag gctgatgcag
     5881 gaggatcgtt taagtccagg agttcaaggc tgcagtgagc tataatcatg ccactgcact
     5941 ccagccaggg agacagctgg aatccatgtc ttataaacaa taataattaa tttttaaaaa
     6001 aagtatttat catccacacc aatcctgggg aagctaacaa tccaccctta acacatttca
     6061 gttttaaaac ctggctgatc tggaatagaa ctttgtccaa attaaattgt agaaatgcta
     6121 tggattcaag agtaaacctg cttgattaat caaacaagct ggaaaactac tgttaatttc
     6181 tttgttgtaa attgatcact gtaattttac cttacatgtc atttatattt tgtatataca
     6241 tgaacatttt aatttccata gcttttaaca ggttgacata atacttctcc atgcagtaag
     6301 tcagtcattt tttccacttg taactgtaat tcaataaaat ttaatcctgt ctggtca
//
