LOCUS       NM_001098484            7716 bp    mRNA    linear   PRI 05-AUG-2024
DEFINITION  Homo sapiens solute carrier family 4 member 4 (SLC4A4), transcript
            variant 1, mRNA.
ACCESSION   NM_001098484
VERSION     NM_001098484.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Chen,H.J., Yu,M.M., Huang,J.C., Lan,F.Y., Liao,H.H., Xu,Z.H.,
            Yu,Y.J., Huang,Y.C. and Chen,F.
  TITLE     SLC4A4 is a novel driver of enzalutamide resistance in prostate
            cancer
  JOURNAL   Cancer Lett 597, 217070 (2024)
   PUBMED   38880227
  REMARK    GeneRIF: SLC4A4 is a novel driver of enzalutamide resistance in
            prostate cancer.
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   Kantaputra,P., Guven,Y., Aksu,B., Kalayci,T., Dogan,C.,
            Intachai,W., Olsen,B., Tongsima,S., Ngamphiw,C. and Noppakun,K.
  TITLE     Distal renal tubular acidosis, autoimmune thyroiditis, enamel
            hypomaturation, and tooth agenesis caused by homozygosity of a
            novel double-nucleotide substitution in SLC4A4
  JOURNAL   J Am Dent Assoc 153 (7), 668-676 (2022)
   PUBMED   35260236
  REMARK    GeneRIF: Distal renal tubular acidosis, autoimmune thyroiditis,
            enamel hypomaturation, and tooth agenesis caused by homozygosity of
            a novel double-nucleotide substitution in SLC4A4.
REFERENCE   3  (bases 1 to 7716)
  AUTHORS   Yamazaki,O., Yamashita,M., Li,J., Ochiai-Homma,F., Yoshida,T.,
            Hirahashi,J., Furukawa,T., Kozuma,K., Fujigaki,Y., Seki,G.,
            Hayashi,M. and Shibata,S.
  TITLE     A novel I551F variant of the Na+/HCO3- cotransporter NBCe1-A shows
            reduced cell surface expression, resulting in diminished transport
            activity
  JOURNAL   Am J Physiol Renal Physiol 321 (6), F771-F784 (2021)
   PUBMED   34719949
  REMARK    GeneRIF: A novel I551F variant of the Na(+)/HCO3(-) cotransporter
            NBCe1-A shows reduced cell surface expression, resulting in
            diminished transport activity.
REFERENCE   4  (bases 1 to 7716)
  AUTHORS   Zheng,Y., Zeng,J., Lin,D., Xia,H., Wang,X., Chen,L., Chen,H.,
            Huang,L. and Zeng,C.
  TITLE     Extracellular vesicles derived from cancer-associated fibroblast
            carries miR-224-5p targeting SLC4A4 to promote the proliferation,
            invasion and migration of colorectal cancer cells
  JOURNAL   Carcinogenesis 42 (9), 1143-1153 (2021)
   PUBMED   34170291
  REMARK    GeneRIF: Extracellular vesicles derived from cancer-associated
            fibroblast carries miR-224-5p targeting SLC4A4 to promote the
            proliferation, invasion and migration of colorectal cancer cells.
REFERENCE   5  (bases 1 to 7716)
  AUTHORS   Brown,M.R., Holmes,H., Rakshit,K., Javeed,N., Her,T.K.,
            Stiller,A.A., Sen,S., Shull,G.E., Prakash,Y.S., Romero,M.F. and
            Matveyenko,A.V.
  TITLE     Electrogenic sodium bicarbonate cotransporter NBCe1 regulates
            pancreatic beta cell function in type 2 diabetes
  JOURNAL   J Clin Invest 131 (17) (2021)
   PUBMED   34623331
  REMARK    GeneRIF: Electrogenic sodium bicarbonate cotransporter NBCe1
            regulates pancreatic beta cell function in type 2 diabetes.
REFERENCE   6  (bases 1 to 7716)
  AUTHORS   Choi,I., Romero,M.F., Khandoudi,N., Bril,A. and Boron,W.F.
  TITLE     Cloning and characterization of a human electrogenic Na+-HCO-3
            cotransporter isoform (hhNBC)
  JOURNAL   Am J Physiol 276 (3), C576-C584 (1999)
   PUBMED   10069984
REFERENCE   7  (bases 1 to 7716)
  AUTHORS   Romero,M.F. and Boron,W.F.
  TITLE     Electrogenic Na+/HCO3- cotransporters: cloning and physiology
  JOURNAL   Annu Rev Physiol 61, 699-723 (1999)
   PUBMED   10099707
  REMARK    Review article
REFERENCE   8  (bases 1 to 7716)
  AUTHORS   Abuladze,N., Lee,I., Newman,D., Hwang,J., Boorer,K., Pushkin,A. and
            Kurtz,I.
  TITLE     Molecular cloning, chromosomal localization, tissue distribution,
            and functional expression of the human pancreatic sodium
            bicarbonate cotransporter
  JOURNAL   J Biol Chem 273 (28), 17689-17695 (1998)
   PUBMED   9651366
REFERENCE   9  (bases 1 to 7716)
  AUTHORS   Burnham,C.E., Amlal,H., Wang,Z., Shull,G.E. and Soleimani,M.
  TITLE     Cloning and functional expression of a human kidney Na+:HCO3-
            cotransporter
  JOURNAL   J Biol Chem 272 (31), 19111-19114 (1997)
   PUBMED   9235899
REFERENCE   10 (bases 1 to 7716)
  AUTHORS   Adams,M.D., Kerlavage,A.R., Fields,C. and Venter,J.C.
  TITLE     3,400 new expressed sequence tags identify diversity of transcripts
            in human brain
  JOURNAL   Nat Genet 4 (3), 256-267 (1993)
   PUBMED   8358434
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC096713.3, AC110783.5,
            AC079230.6 and AC019089.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001098484.2.
            
            Summary: This gene encodes a sodium bicarbonate cotransporter (NBC)
            involved in the regulation of bicarbonate secretion and absorption
            and intracellular pH. Mutations in this gene are associated with
            proximal renal tubular acidosis. Multiple transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Oct 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF011390.1, SRR1660803.244080.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264485.11/ ENSP00000264485.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-143               AC096713.3         6306-6448
            144-217             AC096713.3         55623-55696
            218-397             AC096713.3         74267-74446
            398-533             AC110783.5         20311-20446
            534-694             AC079230.6         9372-9532
            695-874             AC079230.6         16468-16647
            875-951             AC079230.6         57037-57113
            952-1109            AC079230.6         100076-100233
            1110-1197           AC079230.6         107106-107193
            1198-1352           AC079230.6         109849-110003
            1353-1466           AC079230.6         110648-110761
            1467-1641           AC079230.6         112955-113129
            1642-1775           AC079230.6         125904-126037
            1776-2047           AC079230.6         132159-132430
            2048-2118           AC079230.6         146408-146478
            2119-2310           AC079230.6         156961-157152
            2311-2424           AC019089.6         14477-14590
            2425-2586           AC019089.6         16642-16803
            2587-2765           AC019089.6         28765-28943
            2766-2838           AC019089.6         30063-30135
            2839-2907           AC019089.6         37555-37623
            2908-3081           AC019089.6         40127-40300
            3082-3243           AC019089.6         42508-42669
            3244-3340           AC019089.6         46208-46304
            3341-3420           AC019089.6         49419-49498
            3421-7716           AC019089.6         50203-54498
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q13.3"
     gene            1..7716
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /note="solute carrier family 4 member 4"
                     /db_xref="GeneID:8671"
                     /db_xref="HGNC:HGNC:11030"
                     /db_xref="MIM:603345"
     exon            1..143
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            144..217
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     CDS             145..3384
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sodium bicarbonate cotransporter 1 (sodium bicarbonate
                     cotransporter, kidney; sodium bicarbonate cotransporter,
                     pancreas); solute carrier family 4, sodium bicarbonate
                     cotransporter, member 5; electrogenic sodium bicarbonate
                     cotransporter 1; solute carrier family 4, sodium
                     bicarbonate cotransporter, member 4, brain type;
                     Na(+)/HCO3(-) cotransporter; solute carrier family 4
                     (sodium bicarbonate cotransporter), member 4; kidney type
                     Na+/HCO3- cotransporter; pancreatic sodium bicarbonate
                     cotransporter"
                     /codon_start=1
                     /product="electrogenic sodium bicarbonate cotransporter 1
                     isoform 1"
                     /protein_id="NP_001091954.1"
                     /db_xref="CCDS:CCDS43236.1"
                     /db_xref="GeneID:8671"
                     /db_xref="HGNC:HGNC:11030"
                     /db_xref="MIM:603345"
                     /translation="MEDEAVLDRGASFLKHVCDEEEVEGHHTIYIGVHVPKSYRRRRR
                     HKRKTGHKEKKEKERISENYSDKSDIENADESSSSILKPLISPAAERIRFILGEEDDS
                     PAPPQLFTELDELLAVDGQEMEWKETARWIKFEEKVEQGGERWSKPHVATLSLHSLFE
                     LRTCMEKGSIMLDREASSLPQLVEMIVDHQIETGLLKPELKDKVTYTLLRKHRHQTKK
                     SNLRSLADIGKTVSSASRMFTNPDNGSPAMTHRNLTSSSLNDISDKPEKDQLKNKFMK
                     KLPRDAEASNVLVGEVDFLDTPFIAFVRLQQAVMLGALTEVPVPTRFLFILLGPKGKA
                     KSYHEIGRAIATLMSDEVFHDIAYKAKDRHDLIAGIDEFLDEVIVLPPGEWDPAIRIE
                     PPKSLPSSDKRKNMYSGGENVQMNGDTPHDGGHGGGGHGDCEELQRTGRFCGGLIKDI
                     KRKAPFFASDFYDALNIQALSAILFIYLATVTNAITFGGLLGDATDNMQGVLESFLGT
                     AVSGAIFCLFAGQPLTILSSTGPVLVFERLLFNFSKDNNFDYLEFRLWIGLWSAFLCL
                     ILVATDASFLVQYFTRFTEEGFSSLISFIFIYDAFKKMIKLADYYPINSNFKVGYNTL
                     FSCTCVPPDPANISISNDTTLAPEYLPTMSSTDMYHNTTFDWAFLSKKECSKYGGNLV
                     GNNCNFVPDITLMSFILFLGTYTSSMALKKFKTSPYFPTTARKLISDFAIILSILIFC
                     VIDALVGVDTPKLIVPSEFKPTSPNRGWFVPPFGENPWWVCLAAAIPALLVTILIFMD
                     QQITAVIVNRKEHKLKKGAGYHLDLFWVAILMVICSLMALPWYVAATVISIAHIDSLK
                     METETSAPGEQPKFLGVREQRVTGTLVFILTGLSVFMAPILKFIPMPVLYGVFLYMGV
                     ASLNGVQFMDRLKLLLMPLKHQPDFIYLRHVPLRRVHLFTFLQVLCLALLWILKSTVA
                     AIIFPVMILALVAVRKGMDYLFSQHDLSFLDDVIPEKDKKKKEDEKKKKKKKGSLDSD
                     NDDSDCPYSEKVPSIKIPMDIMEQQPFLSDSKPSDRERSPTFLERHTSC"
     exon            218..397
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            398..533
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            534..694
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            695..874
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            875..951
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1109
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1197
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1352
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..1466
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1641
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            1642..1775
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..2047
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..2118
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            2119..2310
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            2311..2424
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            2425..2586
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            2587..2765
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            2766..2838
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            2839..2907
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            2908..3081
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            3082..3243
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            3244..3340
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            3341..3420
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     exon            3421..7716
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7698..7703
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /note="hexamer: AATAAA"
     polyA_site      7716
                     /gene="SLC4A4"
                     /gene_synonym="hhNMC; HNBC1; KNBC; kNBC1; NBC1; NBC2;
                     NBCe1; NBCe1-A; pNBC; PRTAO; SLC4A5"
                     /note="major polyA site"
ORIGIN      
        1 ggcggcgcgc cgggcagcgc ttcggtggcg gcggcggccg cggtggcagc gaaggcggcg
       61 gcggcggcgg cagtggcagt ggccgctgca gccccacact ccgccgccaa actggaggag
      121 cgacggaagc cagaccccag gaggatggag gatgaagctg tcctggacag aggggcttcc
      181 ttcctcaagc atgtgtgtga tgaagaagaa gtagaaggcc accataccat ttacatcgga
      241 gtccatgtgc cgaagagtta caggagaagg agacgtcaca agagaaagac agggcacaaa
      301 gaaaagaagg aaaaggagag aatctctgag aactactctg acaaatcaga tattgaaaat
      361 gctgatgaat ccagcagcag catcctaaaa cctctcatct ctcctgctgc agaacgcatc
      421 cgattcatct tgggagagga ggatgacagc ccagctcccc ctcagctctt cacggaactg
      481 gatgagctgc tggccgtgga tgggcaggag atggagtgga aggaaacagc caggtggatc
      541 aagtttgaag aaaaagtgga acagggtggg gaaagatgga gcaagcccca tgtggccaca
      601 ttgtcccttc atagtttatt tgagctgagg acatgtatgg agaaaggatc catcatgctt
      661 gatcgggagg cttcttctct cccacagttg gtggagatga ttgttgacca tcagattgag
      721 acaggcctat tgaaacctga acttaaggat aaggtgacct atactttgct ccggaagcac
      781 cggcatcaaa ccaagaaatc caaccttcgg tccctggctg acattgggaa gacagtctcc
      841 agtgcaagta ggatgtttac caaccctgat aatggtagcc cagccatgac ccataggaat
      901 ctgacttcct ccagtctgaa tgacatttct gataaaccgg agaaggacca gctgaagaat
      961 aagttcatga aaaaattgcc acgtgatgca gaagcttcca acgtgcttgt tggggaggtt
     1021 gactttttgg atactccttt cattgccttt gttaggctac agcaggctgt catgctgggt
     1081 gccctgactg aagttcctgt gcccacaagg ttcttgttca ttctcttagg tcctaagggg
     1141 aaagccaagt cctaccacga gattggcaga gccattgcca ccctgatgtc tgatgaggtg
     1201 ttccatgaca ttgcttataa agcaaaagac aggcacgacc tgattgctgg tattgatgag
     1261 ttcctagatg aagtcatcgt ccttccacct ggggaatggg atccagcaat taggatagag
     1321 cctcctaaga gtcttccatc ctctgacaaa agaaagaata tgtactcagg tggagagaat
     1381 gttcagatga atggggatac gccccatgat ggaggtcacg gaggaggagg acatggggat
     1441 tgtgaagaat tgcagcgaac tggacggttc tgtggtggac taattaaaga cataaagagg
     1501 aaagcgccat tttttgccag tgatttttat gatgctttaa atattcaagc tctttcggca
     1561 attctcttca tttatctggc aactgtaact aatgctatca cttttggagg actgcttggg
     1621 gatgccactg acaacatgca gggcgtgttg gagagtttcc tgggcactgc tgtctctgga
     1681 gccatctttt gcctttttgc tggtcaacca ctcactattc tgagcagcac cggacctgtc
     1741 ctagtttttg agaggcttct atttaatttc agcaaggaca ataattttga ctatttggag
     1801 tttcgccttt ggattggcct gtggtccgcc ttcctatgtc tcattttggt agccactgat
     1861 gccagcttct tggttcaata cttcacacgt ttcacggagg agggcttttc ctctctgatt
     1921 agcttcatct ttatctatga tgctttcaag aagatgatca agcttgcaga ttactacccc
     1981 atcaactcca acttcaaagt gggctacaac actctctttt cctgtacctg tgtgccacct
     2041 gacccagcta atatctcaat atctaatgac accacactgg ccccagagta tttgccaact
     2101 atgtcttcta ctgacatgta ccataatact acctttgact gggcattttt gtcgaagaag
     2161 gagtgttcaa aatacggagg aaacctcgtc gggaacaact gtaattttgt tcctgatatc
     2221 acactcatgt cttttatcct cttcttggga acctacacct cttccatggc tctgaaaaaa
     2281 ttcaaaacta gtccttattt tccaaccaca gcaagaaaac tgatcagtga ttttgccatt
     2341 atcttgtcca ttctcatctt ttgtgtaata gatgccctag taggcgtgga caccccaaaa
     2401 ctaattgtgc caagtgagtt caagccaaca agtccaaacc gaggttggtt cgttccaccg
     2461 tttggagaaa acccctggtg ggtgtgcctt gctgctgcta tcccggcttt gttggtcact
     2521 atactgattt tcatggacca acaaattaca gctgtgattg taaacaggaa agaacataaa
     2581 ctcaagaaag gagcagggta tcacttggat ctcttttggg tggccatcct catggttata
     2641 tgctccctca tggctcttcc gtggtatgta gctgctacgg tcatctccat tgctcacatc
     2701 gacagtttga agatggagac agagacttct gcacctggag aacaaccaaa gtttctagga
     2761 gtgagggaac aaagagtcac tggaaccctt gtgtttattc tgactggtct gtcagtcttt
     2821 atggctccca tcttgaagtt tatacccatg cctgtactct atggtgtgtt cctgtatatg
     2881 ggagtagcat cccttaatgg tgtgcagttc atggatcgtc tgaagctgct tctgatgcct
     2941 ctgaagcatc agcctgactt catctacctg cgtcatgttc ctctgcgcag agtccacctg
     3001 ttcactttcc tgcaggtgtt gtgtctggcc ctgctttgga tcctcaagtc aacggtggct
     3061 gctatcattt ttccagtaat gatcttggca cttgtagctg tcagaaaagg catggactac
     3121 ctcttctccc agcacgacct cagcttcctg gatgatgtca ttccagaaaa ggacaagaaa
     3181 aagaaggagg atgagaagaa aaagaaaaag aagaagggaa gtctggacag tgacaatgat
     3241 gattctgact gcccatactc agaaaaagtt ccaagtatta aaattccaat ggacatcatg
     3301 gaacagcaac ctttcctaag cgatagcaaa ccttctgaca gagaaagatc accaacattc
     3361 cttgaacgcc acacatcatg ctgataaaat tcctttcctt cagtcactcg gtatgccaag
     3421 tcctcctaga actccagtaa aagttgtgcc tcaaattaga atagaacttg aacctgaaga
     3481 caatgattat ttctggagga gcaagggaac agaaactaca ttgtaacctg tttgtctttc
     3541 ttaaaactga catttgttgt taatgtcatt tgtttttgtt tggctgtttg tttatttttt
     3601 aacttttatt tcgtctcagt ttttggtcac aggccaaata atacagcgct ctctctgctt
     3661 ctctcttgca tagacacaat caagacaata gtgcaccgtt ccttaaaaac agcatctgag
     3721 gaatccccct tttgttctta aactttcaga tgtgtccttt gataaccaaa ttctgtcact
     3781 caagacacag acacgcacag accctgtcct ttgcctctat taagcagagg atggaagtat
     3841 taaggatttt gtaacacctt ttatgaaaat gttgaaggaa cttaaaactt tagctttgga
     3901 gctgtgctta ctggcttgtc tttgtctggt agaacaaacc ttgacctcca gacagagtcc
     3961 cttctcactt atagagctct ccaggactgg aaaaagtgct gctattttaa cttgctcttg
     4021 cttgtaaatc ctaatcttag agttatcaaa agaagaaaaa actgaaggta ctttactccc
     4081 tatagagaaa ccattgccat cattgtagca agtgctggaa tgtccctttt ttcctatgca
     4141 acttttttta accctttaat gaacttatct gttgagtaca ttgaagaata tttttcttcc
     4201 tagattttgt tgtttaaatt atggggccta acctgccact tattttttgt caatttttaa
     4261 aacttttttt taattactgt aaagaaaatg aattttttcc tgcagcagga aacatagttt
     4321 tgagtagttc tacctcttat ttgtagctgc caggctttct gtaaaaattg tattgtatat
     4381 aatgtgattt ttacacatac atacacacac aaatacacaa tctctagggt aagccagaag
     4441 gcaagatcag attaaaaaca ccatgtttct aagcatccat ttttcccttt ctttaaaaga
     4501 aacttaactg ttctatgaag gagattgagg gagaagagac aaactcctat gtcatgagaa
     4561 taaccgatgt tctgataata gtagcatcta ggtacagatg ctggttgtat taccacgtca
     4621 atgtcctatg cagtattgtt agacattttc tcattttgaa atatttgtgt gtttgtgtat
     4681 gtgctctgtg ccatggctgg tgtatatatg tgcaatgtta gaaggcaaaa gagtgatggt
     4741 aggcagaggg caaagtcatt gaatctctta tgccagtttt cataaaaccc aaaccacata
     4801 tgaaaaaatc cattaagggt ccaagaagtc tgtccatatg aaaatgaggg taaatatagt
     4861 ttatttccca ggtatcagtc attataattg atataatagc tctaacatgc aatataaaat
     4921 tcataggagt attaatagcc catttacaca tctataaaat gtaatgggat tgcagagctg
     4981 cagagtacag tgtaacagta ctctcatgca atttttttca ggatgcaaag gcaattattc
     5041 tttgtaagcg ggacatttag aatatatttg tgtacatatt atatgtatgt atatttcaaa
     5101 gtaccacact gaaaattaga catttattaa ccaaatttaa cgtggtattt aaaggtaata
     5161 tttttaatat gatacattac atattgtgaa tgtatactaa aaaaacattt taaatgttaa
     5221 aattataatt tcagattcat ataaccacaa ctgtgatata tcctaactat aaccagttgt
     5281 tgaggggtat actagaagca gaatgaaacc acattttttg gtttgataat atgcacttat
     5341 tgactcccac tcattgttat gttaattaag ttattattct gtctccttgt aattttgatt
     5401 acaaaaattt tattatcctg agttagctgt tacttttaca gtacctgata ctcctaaaac
     5461 ttttaactta tacaaattag tcaataatga ccccaatttt ttcattaaaa taatagtggt
     5521 gaattatatg ttattgtgtt aaaacctcac ttgccaaatt ctggcttcac atttgtattt
     5581 agggctatcc ttaaaatgat gagtctatat tatctagctt tctattaccc taatataaac
     5641 tggtataaga agactttcct tttttcttta tgcatggaag catcaataaa ttgtttaaaa
     5701 accatgtata gtaaattcag cttaacccgt gatcttctta agttaaaggt acttttgttt
     5761 tataaaagct ctagataaaa ctttcttttc tgatcatgaa tcaagtatct gtggtttcat
     5821 gcccctctct atacctttca aagaactcct gaagcaactt aactcatcat ttcagcctct
     5881 gagtagaggt aaaacctatg tgtacttctg tttatgatcc atattgatat ttatgacatg
     5941 aacacagaat agtaccttac atttgctaaa cagacagtta atatcaaatc ctttcaatat
     6001 tctgggaacc cagggaagtt tttaaaaatg tcattacttt caaaggaaca gaagtagtta
     6061 accaaactaa caagcaaaac ctgaggttta cctagtgaca ccaaattatc ggtattttaa
     6121 ctgaatttac ccattgacta agaatgaacc agatttggtg gtggttttgt ttctatgcaa
     6181 actggacaca aattacaaca gtaaattttt ttataagtgc ttctcccttc tccatgatgt
     6241 gacttccgga gataaaggat tcaaaagata aagacaaagt acgctcagag ttgttaacca
     6301 gaaagtcctg gctgtggttg cagaaacact gttggaagaa aagagatgac taagtcaagt
     6361 gtctgcctta tcaaaagagc aaaaatgcct ctggttttgt gtttgggaga aaagtatctt
     6421 ggacgcactg ttttccttga taaaagtcat cttctctact gtgtgaaatg aatacttgga
     6481 attctaattg ttttgtgtgc caggggcagt aatgtccctg cctcttctcc caatcaaggt
     6541 tgaggagtgg ggctggggag aggacttaac tgacttaaga agtaggaaaa caaaaacctc
     6601 tctcctcagc cttccacctc caagagagga ggaaaaacag ttgtctgctg tctgtaattc
     6661 agtttgcgtg tattttatgc tcatgcacca acccatacag agtaaatctt ttatcaactg
     6721 tatactggtg tttaatagag aatgattgtc ttccgagttt tttggttcct tttttaactg
     6781 tgttaaagta cttgaaatgt attgactgct gactatattt taaaaacaaa atgaaataat
     6841 ttgagttgta ttacagaggt tgacattgtt cagggatggg acaaagcctt cttcaatcct
     6901 tttcatacta cttaatgatt ttggtgcagg aacctgagat tttctgattt atatttcatg
     6961 atatttcaca tttgctcttc acagcatgag catgaagccc agtggcacca aatggctggg
     7021 tacaatcaag tgatattttg tagcacctca ctatctgaaa ggccatgagt tttcagatga
     7081 tttcattgag cttcattgca gcctgaaatt ttaaaaaagt tgtgtaatac gccaaccagt
     7141 caagttgtgt tttggccaga gatttagata tgtccaattt cctggctcat ttcattgtgc
     7201 tctatgggta cgtataaaaa gcaagaattc tgtttcctag gcaaacattg caactcaggg
     7261 ctaaagtcat ccagtgaaac ttttagagcc agaagtaact ttgtcccagt cctacaatgt
     7321 gaaaagagtg aatagttgcc tctttttagc cattttcatg gctggtacat attcgtacgc
     7381 attacttttc agaatcaata cgcactttca gatattctta tttttattct cttaagtctt
     7441 tattaacttt ggagagagaa atgatgcatc tttttatttt aaatgaagta gatcaacatg
     7501 gtggaacaaa atgataaaga acagaaaaca tttcaatata ttactaataa ctttttccaa
     7561 tataaatcct aaaattccta taacatagta ttttacagtt ttatgaagct ttctattgtg
     7621 acttttatgg aattaagaga tgaagaagat gagatatttt agcatttata tttttcaaaa
     7681 ttatatgtat acttaaaaat aaagtaactt tatgca
//
