LOCUS       NM_001178015            5578 bp    mRNA    linear   PRI 01-AUG-2024
DEFINITION  Homo sapiens solute carrier family 4 member 10 (SLC4A10),
            transcript variant 1, mRNA.
ACCESSION   NM_001178015
VERSION     NM_001178015.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Maroofian,R., Zamani,M., Kaiyrzhanov,R., Liebmann,L.,
            Karimiani,E.G., Vona,B., Huebner,A.K., Calame,D.G., Misra,V.K.,
            Sadeghian,S., Azizimalamiri,R., Mohammadi,M.H., Zeighami,J.,
            Heydaran,S., Toosi,M.B., Akhondian,J., Babaei,M., Hashemi,N.,
            Schnur,R.E., Suri,M., Setzke,J., Wagner,M., Brunet,T.,
            Grochowski,C.M., Emrick,L., Chung,W.K., Hellmich,U.A., Schmidts,M.,
            Lupski,J.R., Galehdari,H., Severino,M., Houlden,H. and Hubner,C.A.
  TITLE     Biallelic variants in SLC4A10 encoding a sodium-dependent
            bicarbonate transporter lead to a neurodevelopmental disorder
  JOURNAL   Genet Med 26 (3), 101034 (2024)
   PUBMED   38054405
  REMARK    GeneRIF: Biallelic variants in SLC4A10 encoding a sodium-dependent
            bicarbonate transporter lead to a neurodevelopmental disorder.
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Fasham,J., Huebner,A.K., Liebmann,L., Khalaf-Nazzal,R.,
            Maroofian,R., Kryeziu,N., Wortmann,S.B., Leslie,J.S., Ubeyratna,N.,
            Mancini,G.M.S., van Slegtenhorst,M., Wilke,M., Haack,T.B.,
            Shamseldin,H.E., Gleeson,J.G., Almuhaizea,M., Dweikat,I.,
            Abu-Libdeh,B., Daana,M., Zaki,M.S., Wakeling,M.N., McGavin,L.,
            Turnpenny,P.D., Alkuraya,F.S., Houlden,H., Schlattmann,P.,
            Kaila,K., Crosby,A.H., Baple,E.L. and Hubner,C.A.
  TITLE     SLC4A10 mutation causes a neurological disorder associated with
            impaired GABAergic transmission
  JOURNAL   Brain 146 (11), 4547-4561 (2023)
   PUBMED   37459438
  REMARK    GeneRIF: SLC4A10 mutation causes a neurological disorder associated
            with impaired GABAergic transmission.
REFERENCE   3  (bases 1 to 5578)
  AUTHORS   Boger,C.A., Gorski,M., McMahon,G.M., Xu,H., Chang,Y.C., van der
            Most,P.J., Navis,G., Nolte,I.M., de Borst,M.H., Zhang,W., Lehne,B.,
            Loh,M., Tan,S.T., Boerwinkle,E., Grams,M.E., Sekula,P., Li,M.,
            Wilmot,B., Moon,J.G., Scheet,P., Cucca,F., Xiao,X.,
            Lyytikainen,L.P., Delgado,G., Grammer,T.B., Kleber,M.E.,
            Sedaghat,S., Rivadeneira,F., Corre,T., Kutalik,Z., Bergmann,S.,
            Nielson,C.M., Srikanth,P., Teumer,A., Muller-Nurasyid,M.,
            Brockhaus,A.C., Pfeufer,A., Rathmann,W., Peters,A., Matsumoto,M.,
            de Andrade,M., Atkinson,E.J., Robinson-Cohen,C., de Boer,I.H.,
            Hwang,S.J., Heid,I.M., Gogele,M., Concas,M.P., Tanaka,T.,
            Bandinelli,S., Nalls,M.A., Singleton,A., Tajuddin,S.M., Adeyemo,A.,
            Zhou,J., Doumatey,A., McWeeney,S., Murabito,J., Franceschini,N.,
            Flessner,M., Shlipak,M., Wilson,J.G., Chen,G., Rotimi,C.N.,
            Zonderman,A.B., Evans,M.K., Ferrucci,L., Devuyst,O., Pirastu,M.,
            Shuldiner,A., Hicks,A.A., Pramstaller,P.P., Kestenbaum,B.,
            Kardia,S.L.R., Turner,S.T., Study,L.C., Briske,T.E., Gieger,C.,
            Strauch,K., Meisinger,C., Meitinger,T., Volker,U., Nauck,M.,
            Volzke,H., Vollenweider,P., Bochud,M., Waeber,G., Kahonen,M.,
            Lehtimaki,T., Marz,W., Dehghan,A., Franco,O.H., Uitterlinden,A.G.,
            Hofman,A., Taylor,H.A., Chambers,J.C., Kooner,J.S., Fox,C.S.,
            Hitzemann,R., Orwoll,E.S., Pattaro,C., Schlessinger,D., Kottgen,A.,
            Snieder,H., Parsa,A. and Cohen,D.M.
  TITLE     NFAT5 and SLC4A10 Loci Associate with Plasma Osmolality
  JOURNAL   J Am Soc Nephrol 28 (8), 2311-2321 (2017)
   PUBMED   28360221
  REMARK    GeneRIF: genetic variation in SLC4A10 expression and function in
            the central nervous system may affect the regulation of systemic
            water balance
REFERENCE   4  (bases 1 to 5578)
  CONSRTM   Schizophrenia Working Group of the Psychiatric Genomics Consortium
  TITLE     Biological insights from 108 schizophrenia-associated genetic loci
  JOURNAL   Nature 511 (7510), 421-427 (2014)
   PUBMED   25056061
REFERENCE   5  (bases 1 to 5578)
  AUTHORS   Mosbruger,T.L., Duggal,P., Goedert,J.J., Kirk,G.D., Hoots,W.K.,
            Tobler,L.H., Busch,M., Peters,M.G., Rosen,H.R., Thomas,D.L. and
            Thio,C.L.
  TITLE     Large-scale candidate gene analysis of spontaneous clearance of
            hepatitis C virus
  JOURNAL   J Infect Dis 201 (9), 1371-1380 (2010)
   PUBMED   20331378
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 5578)
  AUTHORS   Parker,M.D., Musa-Aziz,R., Rojas,J.D., Choi,I., Daly,C.M. and
            Boron,W.F.
  TITLE     Characterization of human SLC4A10 as an electroneutral Na/HCO3
            cotransporter (NBCn2) with Cl- self-exchange activity
  JOURNAL   J Biol Chem 283 (19), 12777-12788 (2008)
   PUBMED   18319254
  REMARK    GeneRIF: propose to rename NCBE as the second electroneutral
            Na/HCO(3) cotransporter, NBCn2
REFERENCE   7  (bases 1 to 5578)
  AUTHORS   Gurnett,C.A., Veile,R., Zempel,J., Blackburn,L., Lovett,M. and
            Bowcock,A.
  TITLE     Disruption of sodium bicarbonate transporter SLC4A10 in a patient
            with complex partial epilepsy and mental retardation
  JOURNAL   Arch Neurol 65 (4), 550-553 (2008)
   PUBMED   18413482
  REMARK    GeneRIF: SLC4A10 (OMIM 605556), a sodium bicarbonate transporter
            gene with high expression in the cerebral cortex and hippocampus,
            was disrupted by the translocation breakpoint on chromosome 2q24.
REFERENCE   8  (bases 1 to 5578)
  AUTHORS   Jacobs,S., Ruusuvuori,E., Sipila,S.T., Haapanen,A., Damkier,H.H.,
            Kurth,I., Hentschke,M., Schweizer,M., Rudhard,Y., Laatikainen,L.M.,
            Tyynela,J., Praetorius,J., Voipio,J. and Hubner,C.A.
  TITLE     Mice with targeted Slc4a10 gene disruption have small brain
            ventricles and show reduced neuronal excitability
  JOURNAL   Proc Natl Acad Sci U S A 105 (1), 311-316 (2008)
   PUBMED   18165320
REFERENCE   9  (bases 1 to 5578)
  AUTHORS   Wang,C.Z., Yano,H., Nagashima,K. and Seino,S.
  TITLE     The Na+-driven Cl-/HCO3- exchanger. Cloning, tissue distribution,
            and functional characterization
  JOURNAL   J Biol Chem 275 (45), 35486-35490 (2000)
   PUBMED   10993873
REFERENCE   10 (bases 1 to 5578)
  AUTHORS   Yano,H., Wang,C., Yamashita,S., Yokoyama,Y., Yokoi,N. and Seino,S.
  TITLE     Assignment of the human solute carrier family 4, sodium bicarbonate
            cotransporter-like, member 10 gene (SLC4A10) to 2q23-->q24 by in
            situ hybridization and radiation hybrid mapping
  JOURNAL   Cytogenet Cell Genet 89 (3-4), 276-277 (2000)
   PUBMED   10965143
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC337667.1, BC143714.1,
            AL832525.1 and BC051190.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001178015.1.
            
            Summary: This gene belongs to a small family of sodium-coupled
            bicarbonate transporters (NCBTs) that regulate the intracellular pH
            of neurons, the secretion of bicarbonate ions across the choroid
            plexus, and the pH of the brain extracellular fluid. The protein
            encoded by this gene was initially identified as a sodium-driven
            chloride bicarbonate exchanger (NCBE) though there is now evidence
            that its sodium/bicarbonate cotransport activity is independent of
            any chloride ion countertransport under physiological conditions.
            This gene is now classified as a member A10 of the SLC4 family of
            transmembrane solute carriers. Alternative splicing results in
            multiple transcript variants encoding distinct isoforms.[provided
            by RefSeq, May 2010].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.179989.1, BC143714.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2154665
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000446997.6/ ENSP00000393066.1
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-33                DC337667.1         83-115
            34-3659             BC143714.1         1-3626
            3660-5559           AL832525.1         871-2770
            5560-5578           BC051190.1         5548-5566
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q24.2"
     gene            1..5578
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /note="solute carrier family 4 member 10"
                     /db_xref="GeneID:57282"
                     /db_xref="HGNC:HGNC:13811"
                     /db_xref="MIM:605556"
     exon            1..151
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     CDS             104..3460
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /note="isoform 1 is encoded by transcript variant 1;
                     solute carrier family 4, sodium bicarbonate
                     cotransporter-like, member 10; sodium-driven chloride
                     bicarbonate exchanger; solute carrier family 4, sodium
                     bicarbonate transporter, member 10"
                     /codon_start=1
                     /product="sodium-driven chloride bicarbonate exchanger
                     isoform 1"
                     /protein_id="NP_001171486.1"
                     /db_xref="CCDS:CCDS54411.1"
                     /db_xref="GeneID:57282"
                     /db_xref="HGNC:HGNC:13811"
                     /db_xref="MIM:605556"
                     /translation="MEIKDQGAQMEPLLPTRNDEEAVVDRGGTRSILKTHFEKEDLEG
                     HRTLFIGVHVPLGGRKSHRRHRHRGHKHRKRDRERDSGLEDGRESPSFDTPSQRVQFI
                     LGTEDDDEEHIPHDLFTELDEICWREGEDAEWRETARWLKFEEDVEDGGERWSKPYVA
                     TLSLHSLFELRSCILNGTVLLDMHANTLEEIADMVLDQQVSSGQLNEDVRHRVHEALM
                     KQHHHQNQKKLTNRIPIVRSFADIGKKQSEPNSMDKNAGQVVSPQSAPACVENKNDVS
                     RENSTVDFSKGLGGQQKGHTSPCGMKQRHEKGPPHQQEREVDLHFMKKIPPGAEASNI
                     LVGELEFLDRTVVAFVRLSPAVLLQGLAEVPIPTRFLFILLGPLGKGQQYHEIGRSIA
                     TLMTDEVFHDVAYKAKDRNDLVSGIDEFLDQVTVLPPGEWDPSIRIEPPKNVPSQEKR
                     KIPAVPNGTAAHGEAEPHGGHSGPELQRTGRIFGGLILDIKRKAPYFWSDFRDAFSLQ
                     CLASFLFLYCACMSPVITFGGLLGEATEGRISAIESLFGASMTGIAYSLFGGQPLTIL
                     GSTGPVLVFEKILFKFCKEYGLSYLSLRASIGLWTATLCIILVATDASSLVCYITRFT
                     EEAFASLICIIFIYEALEKLFELSEAYPINMHNDLELLTQYSCNCVEPHNPSNGTLKE
                     WRESNISASDIIWENLTVSECKSLHGEYVGRACGHDHPYVPDVLFWSVILFFSTVTLS
                     ATLKQFKTSRYFPTKVRSIVSDFAVFLTILCMVLIDYAIGIPSPKLQVPSVFKPTRDD
                     RGWFVTPLGPNPWWTVIAAIIPALLCTILIFMDQQITAVIINRKEHKLKKGCGYHLDL
                     LMVAVMLGVCSIMGLPWFVAATVLSITHVNSLKLESECSAPGEQPKFLGIREQRVTGL
                     MIFILMGSSVFMTSILKFIPMPVLYGVFLYMGASSLKGIQFFDRIKLFWMPAKHQPDF
                     IYLRHVPLRKVHLFTIIQMSCLGLLWIIKVSRAAIVFPMMVLALVFVRKLMDLLFTKR
                     ELSWLDDLMPESKKKKLEDAEKEEEQSMLAMEDEGTVQLPLEGHYRDDPSVINISDEM
                     SKTALWRNLLITADNSKDKESSFPSKSSPS"
     exon            152..233
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            234..380
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            381..519
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            520..680
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            681..869
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            870..961
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1051
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1209
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1297
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1444
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1545
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1720
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1721..1854
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..2100
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2101..2206
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2207..2368
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2369..2482
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2483..2644
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2645..2896
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2897..2965
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2966..3139
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            3140..3262
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            3263..3330
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            3331..3447
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            3448..3486
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            3487..5578
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5542..5547
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /note="hexamer: AATAAA"
     polyA_site      5578
                     /gene="SLC4A10"
                     /gene_synonym="NBCn2; NCBE"
                     /note="major polyA site"
ORIGIN      
        1 agagctacct gatccgaata ctaagcagag cgagtgccgg gctgagtgta agacactgaa
       61 gacactgcag agcaaggtgc ttattccaga ggcgttacaa aacatggaga ttaaagacca
      121 gggagcccaa atggagccgc tgctgcctac gagaaatgat gaagaagcag ttgtggatag
      181 aggtggaact cgttctattc tcaaaacaca ctttgagaaa gaagatttag aaggtcatcg
      241 aacactattt attggagtac atgtgccctt gggaggaaga aaaagccatc gacgtcacag
      301 gcatcgtggt cataaacaca gaaagagaga cagagaaaga gattcaggat tagaggatgg
      361 aagggagtca ccttcttttg acaccccatc acagagggta cagtttattc ttggaaccga
      421 ggatgatgac gaggaacaca ttcctcatga ccttttcaca gaactggatg agatttgttg
      481 gcgtgaaggt gaggacgctg agtggcgaga aacagccagg tggttgaagt ttgaagaaga
      541 tgtggaagat ggaggagaaa ggtggagcaa gccttatgtg gctactcttt cattgcacag
      601 cttgtttgaa ttgagaagtt gtattctgaa tggaactgtg ttgctggaca tgcatgccaa
      661 cactttagaa gaaattgcag atatggttct tgaccaacaa gtgagctcag gtcagctgaa
      721 tgaagatgta cgccataggg tccatgaggc attgatgaaa cagcatcatc atcagaatca
      781 gaaaaaactc accaacagga ttcccattgt tcgttccttt gctgatattg gcaagaaaca
      841 gtcagaacca aattccatgg acaaaaatgc aggtcaggtt gtttctcctc agtctgctcc
      901 agcctgtgtt gaaaataaaa atgatgttag cagagaaaac agcactgttg actttagcaa
      961 gggactggga ggccaacaaa aggggcatac tagtccatgt gggatgaaac aaaggcatga
     1021 aaaaggacct ccacaccagc aagagagaga ggttgatctg cattttatga aaaagattcc
     1081 tccaggtgct gaagcatcga acatcttagt gggagaactg gagttcttgg atcgaacagt
     1141 agttgcgttt gtcaggttgt ctccagctgt attgcttcaa ggactggctg aagtcccaat
     1201 cccaaccaga tttttgttca ttcttctggg acccctggga aagggtcaac agtaccatga
     1261 gattggcaga tcaattgcaa ccctaatgac agatgaggta tttcatgatg ttgcctataa
     1321 agctaaagat cgtaatgact tggtatcagg aattgatgag tttctggatc aggttactgt
     1381 tctccctcct ggagaatggg atccaagcat tcgaatagag cctcccaaaa atgttccttc
     1441 ccaggagaag aggaagattc ctgctgtacc aaatggaaca gcagctcatg gggaagcaga
     1501 gccccacgga ggacatagtg gacctgaact ccagcgaact ggaaggattt ttgggggact
     1561 tattttagat atcaaaagaa aagctccata cttctggagt gacttcagag atgctttcag
     1621 cctgcagtgc ttagcatctt ttctatttct ctactgcgcg tgtatgtctc ctgtcatcac
     1681 gtttggagga ctgctgggag aagcaactga agggcgtata agtgcaattg aatctctctt
     1741 tggagcatcc atgaccggga tagcctattc tctctttggt ggacagcctc ttaccatatt
     1801 aggcagtaca ggaccagttt tggtgtttga aaagattttg tttaaatttt gcaaagaata
     1861 tgggctgtca tacctatctt taagagctag cattggactt tggactgcaa ctctatgtat
     1921 catacttgtg gccacagatg ctagttccct tgtctgctac atcactcggt ttactgaaga
     1981 agcttttgct tccctgattt gcatcatttt catttatgag gccctggaga agttgtttga
     2041 actcagtgaa gcatatccaa tcaacatgca taatgatctg gaactgctga cacaatactc
     2101 gtgtaactgt gtggaaccgc ataatcccag caatggcaca ttgaaggaat ggagggaatc
     2161 caatatttct gcctctgaca taatttggga gaacctaact gtgtcagaat gcaaatcatt
     2221 gcatggagag tatgttggac gggcctgtgg ccatgatcac ccatatgttc cagatgttct
     2281 attttggtct gtgatcctgt tcttttccac agttactctg tcagccaccc tgaagcagtt
     2341 caagactagc agatattttc caaccaaggt tcgatccata gtgagtgact ttgctgtctt
     2401 tcttacaatt ctgtgtatgg ttttaattga ctatgccatt gggatcccat ctccaaaact
     2461 acaagtacca agtgttttca agcccactag agatgatcgt ggctggtttg ttacgccttt
     2521 aggtccaaac ccatggtgga cagtaatagc tgctataatt ccagctctgc tttgtactat
     2581 tctaattttt atggaccaac agattacagc tgtcatcatc aacaggaaag agcataagct
     2641 aaagaaaggt tgtgggtacc atctggacct attaatggtg gctgtcatgc tcggtgtatg
     2701 ctccatcatg ggcctgccat ggtttgtggc tgccacagtc ctctccatca ctcatgtcaa
     2761 tagcctaaaa ctggaatcag aatgctcagc tccaggagaa caacccaaat ttctcggcat
     2821 tcgggagcaa agggttactg ggcttatgat ttttattctt atgggttcat cagtctttat
     2881 gaccagtatt ctgaagttta ttcccatgcc agtgctatat ggagtgtttc tttatatggg
     2941 tgcttcatct ctaaagggaa ttcagttctt tgataggata aagctcttct ggatgccggc
     3001 aaaacatcaa ccagatttta tatacctaag gcacgtaccg cttcgaaaag tgcatctctt
     3061 cacaattatt cagatgagtt gccttggcct tttgtggata ataaaagttt caagagctgc
     3121 tattgtcttt cccatgatgg tgttagccct ggtatttgta agaaagttga tggacttgtt
     3181 gttcacgaag cgggaactca gctggttgga tgatttgatg cccgagagta agaaaaagaa
     3241 actggaagat gctgaaaaag aagaagaaca aagtatgcta gctatggaag atgagggcac
     3301 agtacaactc ccattggaag ggcactatag agatgatcca tctgtgatca atatatctga
     3361 tgaaatgtca aagactgcct tgtggaggaa ccttctgatt actgccgata actcaaaaga
     3421 taaggagtca agctttcctt ccaaaagctc cccttcctaa tcactctaga agctgattcc
     3481 ccaaagcatt gaaagccgaa aagagaagaa agctgactca gggaaaggtg ttgacaggga
     3541 gacttgtcta tgactcgatc ttcaatttat tttttacata tatatgagaa gagtgtcaca
     3601 attattaata aaactgcttt gatcatgtat tgtaaattct gtccctcaac ccaaatccac
     3661 cttcatactg taagtagtgc aatacttgtt tcatttctgt gtttaaactt ctgagcagtg
     3721 agacatccct gtgagcagat acaatagcca atgcaagaat ctgtgtgttc cttgctgtac
     3781 gttagacatt tgtaaactgg attctgattg tcagttttat gagagcaata gcttccttaa
     3841 agagataagt catatttacc tagtttgtat tttcctactt tagtgacctg aagatgcctg
     3901 ataatttcat tcagaagaat ttttgaaagg tagtcttact tctttttagt ttttatagct
     3961 tagcattagt gacttatttc aaaagaccca aatcaaaaag ttagtttgaa agcatttttt
     4021 aataattgta tttatgcatt tccttgattt aatatgataa atttaatact taacaattta
     4081 tatgtaacta aaacttaaag tcatttgaaa aatatataga aacctattta caacttgtta
     4141 aggacaatca gacataatgc agagttaagt agtatttgct taaaattcaa gttgtgacta
     4201 atgatcaaat actaggcttg tacgaaatgc tttagaaaaa ctttgtaaca gttttgtggg
     4261 atttttcaat ataaaccttt atcagaaata tactaagttt gtctcccact gacaacagat
     4321 gttttccaaa taaacatatt ctatacatac ttgtggaatg ccacatggtg aatcattgta
     4381 tatgaaattc cactcctgta cagttactct gcagctaatg gtcatgcact gcttaatgct
     4441 ggtcctgaat catgttctca tgttagacca acagctctcc aattgtcatt ttttttctgc
     4501 agagtttttt ttttccactt ttaaattaaa tgcatgttgt ggaaaaacag tcttttaaaa
     4561 tgaaatttca gattccattt gagaaggttc tgtagatatt tcagtccata taaaataata
     4621 catctttact aaacttatat aaggggagag aaagttatga agttttggac attactaaaa
     4681 gtacagtatt tgatttcact ttcaatgaat gtgaagttaa taaaactaaa tctcataatg
     4741 ctcttggttc ctaagaatga gtagtaatca tcaactttat aatactccaa tattccgttt
     4801 tataataatt cagagccctg tggcttttac acaccgttaa ttatgtactc tgttggaagt
     4861 gcacatgaaa agtgaagaaa agttcctctt gtgattaaac taatgggagg aaataaatca
     4921 acaaagtctc cattaagttc tacattttga gaccttttaa aaattcccct cacaattctt
     4981 taaggagccc ccctttttat ggaacatgag cctaaaaatt atagaaagaa gaattttaag
     5041 ttaataaagt ttgtatttat aaatgctgaa aaaatacaga aactttctgt tccaaatgtg
     5101 ttgcctttgt gtattttata atacagatac tacattgtaa acatttccat tgttttatga
     5161 tttagccagt gattccccaa agcagcctct tagtgtttta atatattaat aactgttttg
     5221 ttaaaaatga tcatagtgaa tttaaatctt cacatgatca cctatttgaa taagcaatca
     5281 tatccaatga aattctgtat ttctgagtat ttttatagtc attttgttct tgtgtgaatt
     5341 ttaaagctat ccctatgtta atcctaatat tttgaaatca tataaaatat aataaaaatg
     5401 tagtattata tatttacttc taatttcaga ttcctggtca aaattactaa atatcttgaa
     5461 tgtaatttag tgccaagttt aaataatgtg taaatgtgac taggatattg tgtttttcac
     5521 aattaagaaa tgttatgtgg aaataaatat ttatcctaac ttccttgcac attttaaa
//
