LOCUS       NM_000342               4954 bp    mRNA    linear   PRI 09-OCT-2024
DEFINITION  Homo sapiens solute carrier family 4 member 1 (Diego blood group)
            (SLC4A1), mRNA.
ACCESSION   NM_000342
VERSION     NM_000342.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Mungara,P., Waiss,M., Hartwig,S., Burger,D. and Cordat,E.
  TITLE     Unraveling the molecular landscape of kAE1: a narrative review
  JOURNAL   Can J Physiol Pharmacol 102 (7), 396-407 (2024)
   PUBMED   38669699
  REMARK    GeneRIF: Unraveling the molecular landscape of kAE1: a narrative
            review.
            Review article
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Guerra Hernandez,N.E., Gomez Tenorio,C., Mendez Silva,L.P.,
            Moraleda Mesa,T., Escobar,L.I., Salvador,C., Vargas Poussou,R. and
            Garcia Nieto,V.M.
  TITLE     Autosomal dominant distal renal tubular acidosis in two pediatric
            patients with mutations in the SLC4A1 gene. Can the maximum urinary
            pCO2 test be normal?
  JOURNAL   Nefrologia (Engl Ed) 43 (4), 484-490 (2023)
   PUBMED   37775346
  REMARK    GeneRIF: Autosomal dominant distal renal tubular acidosis in two
            pediatric patients with mutations in the SLC4A1 gene. Can the
            maximum urinary pCO2 test be normal?
REFERENCE   3  (bases 1 to 4954)
  AUTHORS   Deejai,N., Sawasdee,N., Nettuwakul,C., Wanachiwanawin,W.,
            Sritippayawan,S., Yenchitsomanus,P.T. and Rungroj,N.
  TITLE     Impaired trafficking and instability of mutant kidney anion
            exchanger 1 proteins associated with autosomal recessive distal
            renal tubular acidosis
  JOURNAL   BMC Med Genomics 15 (1), 228 (2022)
   PUBMED   36320073
  REMARK    GeneRIF: Impaired trafficking and instability of mutant kidney
            anion exchanger 1 proteins associated with autosomal recessive
            distal renal tubular acidosis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4954)
  AUTHORS   Bruce,L.J., Beckmann,R., Ribeiro,M.L., Peters,L.L., Chasis,J.A.,
            Delaunay,J., Mohandas,N., Anstee,D.J. and Tanner,M.J.
  TITLE     A band 3-based macrocomplex of integral and peripheral proteins in
            the RBC membrane
  JOURNAL   Blood 101 (10), 4180-4188 (2003)
   PUBMED   12531814
REFERENCE   5  (bases 1 to 4954)
  AUTHORS   Zhang,D., Kiyatkin,A., Bolin,J.T. and Low,P.S.
  TITLE     Crystallographic structure and functional interpretation of the
            cytoplasmic domain of erythrocyte membrane band 3
  JOURNAL   Blood 96 (9), 2925-2933 (2000)
   PUBMED   11049968
REFERENCE   6  (bases 1 to 4954)
  AUTHORS   Peters,L.L., Shivdasani,R.A., Liu,S.C., Hanspal,M., John,K.M.,
            Gonzalez,J.M., Brugnara,C., Gwynn,B., Mohandas,N., Alper,S.L.,
            Orkin,S.H. and Lux,S.E.
  TITLE     Anion exchanger 1 (band 3) is required to prevent erythrocyte
            membrane surface loss but not to form the membrane skeleton
  JOURNAL   Cell 86 (6), 917-927 (1996)
   PUBMED   8808627
REFERENCE   7  (bases 1 to 4954)
  AUTHORS   Alexander,R.T., Law,L., Gil-Pena,H., Greenbaum,L.A. and Santos,F.
  TITLE     Hereditary Distal Renal Tubular Acidosis
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   31600044
REFERENCE   8  (bases 1 to 4954)
  AUTHORS   Bennett,V. and Stenbuck,P.J.
  TITLE     The membrane attachment protein for spectrin is associated with
            band 3 in human erythrocyte membranes
  JOURNAL   Nature 280 (5722), 468-473 (1979)
   PUBMED   379653
REFERENCE   9  (bases 1 to 4954)
  AUTHORS   Drickamer,L.K.
  TITLE     Orientation of the band 3 polypeptide from human erythrocyte
            membranes. Identification of NH2-terminal sequence and site of
            carbohydrate attachment
  JOURNAL   J Biol Chem 253 (20), 7242-7248 (1978)
   PUBMED   701248
REFERENCE   10 (bases 1 to 4954)
  AUTHORS   Lewis,M., Kaita,H., McAlpine,P.J., Fletcher,J. and Moulds,J.J.
  TITLE     A 'new' blood group antigen Fra: incidence, inheritance and genetic
            linkage analysis
  JOURNAL   Vox Sang 35 (4), 251-254 (1978)
   PUBMED   695442
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC003043.2, X12609.1 and
            AC003102.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000342.3.
            
            Summary: The protein encoded by this gene is part of the anion
            exchanger (AE) family and is expressed in the erythrocyte plasma
            membrane, where it functions as a chloride/bicarbonate exchanger
            involved in carbon dioxide transport from tissues to lungs. The
            protein comprises two domains that are structurally and
            functionally distinct. The N-terminal 40kDa domain is located in
            the cytoplasm and acts as an attachment site for the red cell
            skeleton by binding ankyrin. The glycosylated C-terminal
            membrane-associated domain contains 12-14 membrane spanning
            segments and carries out the stilbene disulphonate-sensitive
            exchange transport of anions. The cytoplasmic tail at the extreme
            C-terminus of the membrane domain binds carbonic anhydrase II. The
            encoded protein associates with the red cell membrane protein
            glycophorin A and this association promotes the correct folding and
            translocation of the exchanger. This protein is predominantly
            dimeric but forms tetramers in the presence of ankyrin. Many
            mutations in this gene are known in man, and these mutations can
            lead to two types of disease: destabilization of red cell membrane
            leading to hereditary spherocytosis, and defective kidney acid
            secretion leading to distal renal tubular acidosis. Other mutations
            that do not give rise to disease result in novel blood group
            antigens, which form the Diego blood group system. Southeast Asian
            ovalocytosis (SAO, Melanesian ovalocytosis) results from the
            heterozygous presence of a deletion in the encoded protein and is
            common in areas where Plasmodium falciparum malaria is endemic. One
            null mutation in this gene is known, resulting in very severe
            anemia and nephrocalcinosis. [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments and published data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X12609.1, M27819.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1966682,
                                           SAMEA1968832 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262418.12/ ENSP00000262418.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AC003043.2         120451-120451
            2-315               X12609.1           2-315
            316-316             AC003043.2         127010-127010
            317-3475            X12609.1           317-3475
            3476-4954           AC003102.1         28271-29749         c
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.31"
     gene            1..4954
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /note="solute carrier family 4 member 1 (Diego blood
                     group)"
                     /db_xref="GeneID:6521"
                     /db_xref="HGNC:HGNC:11027"
                     /db_xref="MIM:109270"
     exon            1..82
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            83..165
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     CDS             151..2886
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /note="Waldner blood group; anion exchange protein 1;
                     Swann blood group; Froese blood group; Wright blood group;
                     erythrocyte membrane protein band 3; band 3 anion
                     exchanger; solute carrier family 4, anion exchanger,
                     number 1; erythroid anion exchange protein; anion
                     exchanger-1; solute carrier family 4, anion exchanger,
                     member 1 (erythrocyte membrane protein band 3, Diego blood
                     group); solute carrier family 4 (anion exchanger), member
                     1 (Diego blood group)"
                     /codon_start=1
                     /product="band 3 anion transport protein"
                     /protein_id="NP_000333.1"
                     /db_xref="CCDS:CCDS11481.1"
                     /db_xref="GeneID:6521"
                     /db_xref="HGNC:HGNC:11027"
                     /db_xref="MIM:109270"
                     /translation="MEELQDDYEDMMEENLEQEEYEDPDIPESQMEEPAAHDTEATAT
                     DYHTTSHPGTHKVYVELQELVMDEKNQELRWMEAARWVQLEENLGENGAWGRPHLSHL
                     TFWSLLELRRVFTKGTVLLDLQETSLAGVANQLLDRFIFEDQIRPQDREELLRALLLK
                     HSHAGELEALGGVKPAVLTRSGDPSQPLLPQHSSLETQLFCEQGDGGTEGHSPSGILE
                     KIPPDSEATLVLVGRADFLEQPVLGFVRLQEAAELEAVELPVPIRFLFVLLGPEAPHI
                     DYTQLGRAAATLMSERVFRIDAYMAQSRGELLHSLEGFLDCSLVLPPTDAPSEQALLS
                     LVPVQRELLRRRYQSSPAKPDSSFYKGLDLNGGPDDPLQQTGQLFGGLVRDIRRRYPY
                     YLSDITDAFSPQVLAAVIFIYFAALSPAITFGGLLGEKTRNQMGVSELLISTAVQGIL
                     FALLGAQPLLVVGFSGPLLVFEEAFFSFCETNGLEYIVGRVWIGFWLILLVVLVVAFE
                     GSFLVRFISRYTQEIFSFLISLIFIYETFSKLIKIFQDHPLQKTYNYNVLMVPKPQGP
                     LPNTALLSLVLMAGTFFFAMMLRKFKNSSYFPGKLRRVIGDFGVPISILIMVLVDFFI
                     QDTYTQKLSVPDGFKVSNSSARGWVIHPLGLRSEFPIWMMFASALPALLVFILIFLES
                     QITTLIVSKPERKMVKGSGFHLDLLLVVGMGGVAALFGMPWLSATTVRSVTHANALTV
                     MGKASTPGAAAQIQEVKEQRISGLLVAVLVGLSILMEPILSRIPLAVLFGIFLYMGVT
                     SLSGIQLFDRILLLFKPPKYHPDVPYVKRVKTWRMHLFTGIQIICLAVLWVVKSTPAS
                     LALPFVLILTVPLRRVLLPLIFRNVELQCLDADDAKATFDEEEGRDEYDEVAMPV"
     exon            166..256
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            257..318
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            319..499
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            500..635
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            636..759
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            760..844
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            845..1026
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1237
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1432
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1581
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            1582..1776
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1950
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            1951..2040
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            2041..2207
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            2208..2461
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            2462..2631
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            2632..2805
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     exon            2806..4954
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4934..4939
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /note="hexamer: AATAAA"
     polyA_site      4954
                     /gene="SLC4A1"
                     /gene_synonym="AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR;
                     RTA1A; SAO; SPH4; SW; WD; WD1; WR"
                     /note="major polyA site"
ORIGIN      
        1 agaacgagtg ggaacgtagc tggtcgcaga gggcaccagc ggctgcagga cttcaccaag
       61 ggaccctgag gctcgtgagc agggacccgc ggtgcgggtt atgctggggg ctcagatcac
      121 cgtagacaac tggacactca ggaccacgcc atggaggagc tgcaggatga ttatgaagac
      181 atgatggagg agaatctgga gcaggaggaa tatgaagacc cagacatccc cgagtcccag
      241 atggaggagc cggcagctca cgacaccgag gcaacagcca cagactacca caccacatca
      301 cacccgggta cccacaaggt ctatgtggag ctgcaggagc tggtgatgga cgaaaagaac
      361 caggagctga gatggatgga ggcggcgcgc tgggtgcaac tggaggagaa cctgggggag
      421 aatggggcct ggggccgccc gcacctctct cacctcacct tctggagcct cctagagctg
      481 cgtagagtct tcaccaaggg tactgtcctc ctagacctgc aagagacctc cctggctgga
      541 gtggccaacc aactgctaga caggtttatc tttgaagacc agatccggcc tcaggaccga
      601 gaggagctgc tccgggccct gctgcttaaa cacagccacg ctggagagct ggaggccctg
      661 gggggtgtga agcctgcagt cctgacacgc tctggggatc cttcacagcc tctgctcccc
      721 caacactcct cactggagac acagctcttc tgtgagcagg gagatggggg cacagaaggg
      781 cactcaccat ctggaattct ggaaaagatt cccccggatt cagaggccac gttggtgcta
      841 gtgggccgcg ccgacttcct ggagcagccg gtgctgggct tcgtgaggct gcaggaggca
      901 gcggagctgg aggcggtgga gctgccggtg cctatacgct tcctctttgt gttgctggga
      961 cctgaggccc cccacatcga ttacacccag cttggccggg ctgctgccac cctcatgtca
     1021 gagagggtgt tccgcataga tgcctacatg gctcagagcc gaggggagct gctgcactcc
     1081 ctagagggct tcctggactg cagcctagtg ctgcctccca ccgatgcccc ctccgagcag
     1141 gcactgctca gtctggtgcc tgtgcagagg gagctacttc gaaggcgcta tcagtccagc
     1201 cctgccaagc cagactccag cttctacaag ggcctagact taaatggggg cccagatgac
     1261 cctctgcagc agacaggcca gctcttcggg ggcctggtgc gtgatatccg gcgccgctac
     1321 ccctattacc tgagtgacat cacagatgca ttcagccccc aggtcctggc tgccgtcatc
     1381 ttcatctact ttgctgcact gtcacccgcc atcaccttcg gcggcctcct gggagaaaag
     1441 acccggaacc agatgggagt gtcggagctg ctgatctcca ctgcagtgca gggcattctc
     1501 ttcgccctgc tgggggctca gcccctgctt gtggtcggct tctcaggacc cctgctggtg
     1561 tttgaggaag ccttcttctc gttctgcgag accaacggtc tagagtacat cgtgggccgc
     1621 gtgtggatcg gcttctggct catcctgctg gtggtgttgg tggtggcctt cgagggtagc
     1681 ttcctggtcc gcttcatctc ccgctatacc caggagatct tctccttcct catttccctc
     1741 atcttcatct atgagacttt ctccaagctg atcaagatct tccaggacca cccactacag
     1801 aagacttata actacaacgt gttgatggtg cccaaacctc agggccccct gcccaacaca
     1861 gccctcctct cccttgtgct catggccggt accttcttct ttgccatgat gctgcgcaag
     1921 ttcaagaaca gctcctattt ccctggcaag ctgcgtcggg tcatcgggga cttcggggtc
     1981 cccatctcca tcctgatcat ggtcctggtg gatttcttca ttcaggatac ctacacccag
     2041 aaactctcgg tgcctgatgg cttcaaggtg tccaactcct cagcccgggg ctgggtcatc
     2101 cacccactgg gcttgcgttc cgagtttccc atctggatga tgtttgcctc cgccctgcct
     2161 gctctgctgg tcttcatcct catattcctg gagtctcaga tcaccacgct gattgtcagc
     2221 aaacctgagc gcaagatggt caagggctcc ggcttccacc tggacctgct gctggtagta
     2281 ggcatgggtg gggtggccgc cctctttggg atgccctggc tcagtgccac caccgtgcgt
     2341 tccgtcaccc atgccaacgc cctcactgtc atgggcaaag ccagcacccc aggggctgca
     2401 gcccagatcc aggaggtcaa agagcagcgg atcagtggac tcctggtcgc tgtgcttgtg
     2461 ggcctgtcca tcctcatgga gcccatcctg tcccgcatcc ccctggctgt actgtttggc
     2521 atcttcctct acatgggggt cacgtcgctc agcggcatcc agctctttga ccgcatcttg
     2581 cttctgttca agccacccaa gtatcaccca gatgtgccct acgtcaagcg ggtgaagacc
     2641 tggcgcatgc acttattcac gggcatccag atcatctgcc tggcagtgct gtgggtggtg
     2701 aagtccacgc cggcctccct ggccctgccc ttcgtcctca tcctcactgt gccgctgcgg
     2761 cgcgtcctgc tgccgctcat cttcaggaac gtggagcttc agtgtctgga tgctgatgat
     2821 gccaaggcaa cctttgatga ggaggaaggt cgggatgaat acgacgaagt ggccatgcct
     2881 gtgtgagggg cgggcccagg ccctagaccc tcccccacca ttccacatcc ccaccttcca
     2941 aggaaaagca gaagttcatg ggcacctcat ggactccagg atcctcctgg agcagcagct
     3001 gaggccccag ggctgtgggt ggggaaggaa ggcgtgtcca ggagaccttc cacaaagggt
     3061 agcctggctt ttctggctgg ggatggccga tggggcccac attagggggt ttgttgcaca
     3121 gtccctcctg ttgccacact ttcactgggg atcccgtgct ggaagactta gatctgagcc
     3181 ctccctcttc ccagcacagg caggggtaga agcaaaggca ggaggtgggt gagcgggtgg
     3241 ggtgcttgct gtgtgacctt gggcaagtcc cttgaccttt ccagcctata tttcctcttc
     3301 tgtaaaatgg gtatattgat gataataccc acattacagg atggttactg aggaccaaag
     3361 atacatgtaa aatagggctt tgtaaactcc acagggactg ttctatagca gtcatcattt
     3421 gtctttgaac gtacccaagg tcacatagct gggatttgaa ctgagccgtg cagctgggat
     3481 ttgaaccagg ccttctgatt tcaaggtccg agctctgtcc tctgtcagtc atgcgtccac
     3541 tttcccttcc cctgtgactc ctcccttccc cactctgctc ccagccccta ccttgagacc
     3601 ctcttctctg ggcccagaga gaggcgtcct ggtgaggaca aggtacaggc aaggatgatc
     3661 cagggattgg gcctgggact caggcctcct aagtgtttgg ttcctccctc caaacactca
     3721 ttagttcact cattcattca ttccacaaac atttactgag ggccccggaa tcagtggact
     3781 ccgaggggac tgagacaagc cctgccctgg ggtgggggtg gggggcaagg tacagttgat
     3841 tctacatttg gatagggagt gggggagggt gggaaggtag gggcgggaga gtgagggggt
     3901 ttgtaattta ttaattgcgt attttctaag agttttcaac atagtttggc ttcacacaca
     3961 acttcaggcc cctcatttga gagccattat cctcaactcc atctaaactg aatcttgggg
     4021 agaacccaga tctgaccaat tggggtagga gacagcaggc tctccaagaa catgggcaaa
     4081 tttatttttt tataaaacaa aaagataaaa agagttgaaa gacgtgaaag tggtgagaga
     4141 tggaggaaac agaatcagga agtggtagaa aagagaggag gtggctgggc gcagtggctc
     4201 acgtttgtaa tcccagcact ttgggaggcc aagttgggcg gatcatttga ggtcaggagt
     4261 ttgagaccag cctggccaac atggtgaaac cccgttacta ctaaaaatac aaaaattagc
     4321 tgggtgtctc gtggcaggca cctgtaatcc cagctactta gaaggctgag gcaagagaat
     4381 cacctgaacc caggaggtgg aggttgcagt gagccaagat tgcaccactg cactccagcc
     4441 tgggcaacag agcgagaccc tgtctcaaaa aaaaaaaaaa aaaaaaaaaa aaaacggaag
     4501 gaaacatcag ccttgggggc cacagactca acatgtgtgt gtggtggggt tccagcccaa
     4561 catagagtaa cattatttgt acctcccagg ctagctcagt ccatgggagg ctctcctgtc
     4621 cctgaaagct gacacccacc tttcaccact tcgcccatgc tacagttcag tttcctcgtc
     4681 tgtaaaatgg ggatgataat ggtacctacc ttgcagtgtt gttataagga ttaaaggaga
     4741 cagtgcaaga aaaggccttg gttggtgaag agcccaacct cggaggggag ctgctgggat
     4801 cctccttatc ttgactggga tgtccctgtc tccccctccc cttgctcctt gaacatggcc
     4861 aaggaaagtg aaaaacaaaa attattcact ctgctagcac ccttcccctt gatgcctggg
     4921 aataggtttt gccaataaac gtatctgtgt tgga
//
