LOCUS       NM_080669               6492 bp    mRNA    linear   PRI 10-DEC-2024
DEFINITION  Homo sapiens solute carrier family 46 member 1 (SLC46A1),
            transcript variant 1, mRNA.
ACCESSION   NM_080669
VERSION     NM_080669.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Wang,D., Wu,H., Yang,J., Li,M., Ling,C., Gao,Z., Lu,H., Shen,H. and
            Tang,Y.
  TITLE     Loss of SLC46A1 decreases tumor iron content in hepatocellular
            carcinoma
  JOURNAL   Hepatol Commun 6 (10), 2914-2924 (2022)
   PUBMED   35811443
  REMARK    GeneRIF: Loss of SLC46A1 decreases tumor iron content in
            hepatocellular carcinoma.
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Narawa,T., Narita,Y., Hosokawa,S. and Itoh,T.
  TITLE     Functional role of serine 318 of the proton-coupled folate
            transporter in methotrexate transport
  JOURNAL   Drug Metab Pharmacokinet 41, 100421 (2021)
   PUBMED   34619546
REFERENCE   3  (bases 1 to 6492)
  AUTHORS   Parker,J.L., Deme,J.C., Kuteyi,G., Wu,Z., Huo,J., Goldman,I.D.,
            Owens,R.J., Biggin,P.C., Lea,S.M. and Newstead,S.
  TITLE     Structural basis of antifolate recognition and transport by PCFT
  JOURNAL   Nature 595 (7865), 130-134 (2021)
   PUBMED   34040256
  REMARK    GeneRIF: Structural basis of antifolate recognition and transport
            by PCFT.
REFERENCE   4  (bases 1 to 6492)
  AUTHORS   O'Connor,C., Wallace-Povirk,A., Ning,C., Fruhauf,J., Tong,N.,
            Gangjee,A., Matherly,L.H. and Hou,Z.
  TITLE     Folate transporter dynamics and therapy with classic and
            tumor-targeted antifolates
  JOURNAL   Sci Rep 11 (1), 6389 (2021)
   PUBMED   33737637
  REMARK    GeneRIF: Folate transporter dynamics and therapy with classic and
            tumor-targeted antifolates.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6492)
  AUTHORS   Schaer,C.A., Vallelian,F., Imhof,A., Schoedon,G. and Schaer,D.J.
  TITLE     Heme carrier protein (HCP-1) spatially interacts with the CD163
            hemoglobin uptake pathway and is a target of inflammatory
            macrophage activation
  JOURNAL   J Leukoc Biol 83 (2), 325-333 (2008)
   PUBMED   17947394
  REMARK    GeneRIF: HCP-1 expression in human macrophages is downregulated by
            TLR agonists & IFN-gamma, upregulated by dexamethasone. In early
            endosomes, it colocalizes with endocytosed Hb-haptoglobin (Hp)
            complexes, taken up by the CD163 scavenger receptor pathway.
REFERENCE   6  (bases 1 to 6492)
  AUTHORS   Sharma,S., Dimasi,D., Broer,S., Kumar,R. and Della,N.G.
  TITLE     Heme carrier protein 1 (HCP1) expression and functional analysis in
            the retina and retinal pigment epithelium
  JOURNAL   Exp Cell Res 313 (6), 1251-1259 (2007)
   PUBMED   17335806
  REMARK    GeneRIF: Our data revealed low-level wider expression of human HCP1
            transcript in multiple tissues suggesting that it is responsible
            for heme transport in the body, not the intestine alone.
REFERENCE   7  (bases 1 to 6492)
  AUTHORS   Latunde-Dada,G.O., Takeuchi,K., Simpson,R.J. and McKie,A.T.
  TITLE     Haem carrier protein 1 (HCP1): Expression and functional studies in
            cultured cells
  JOURNAL   FEBS Lett 580 (30), 6865-6870 (2006)
   PUBMED   17156779
  REMARK    GeneRIF: HCP1 expression in different cells implies a functional
            role in tissues other than the duodenum
REFERENCE   8  (bases 1 to 6492)
  AUTHORS   Qiu,A., Jansen,M., Sakaris,A., Min,S.H., Chattopadhyay,S., Tsai,E.,
            Sandoval,C., Zhao,R., Akabas,M.H. and Goldman,I.D.
  TITLE     Identification of an intestinal folate transporter and the
            molecular basis for hereditary folate malabsorption
  JOURNAL   Cell 127 (5), 917-928 (2006)
   PUBMED   17129779
  REMARK    GeneRIF: A loss-of-function mutation in PCFT/HCP1 is the molecular
            basis for hereditary folate malabsorption in a family with this
            disease.
REFERENCE   9  (bases 1 to 6492)
  AUTHORS   Shayeghi,M., Latunde-Dada,G.O., Oakhill,J.S., Laftah,A.H.,
            Takeuchi,K., Halliday,N., Khan,Y., Warley,A., McCann,F.E.,
            Hider,R.C., Frazer,D.M., Anderson,G.J., Vulpe,C.D., Simpson,R.J.
            and McKie,A.T.
  TITLE     Identification of an intestinal heme transporter
  JOURNAL   Cell 122 (5), 789-801 (2005)
   PUBMED   16143108
REFERENCE   10 (bases 1 to 6492)
  AUTHORS   Goldman,I.D.
  TITLE     Hereditary Folate Malabsorption
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301716
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA188147.1, DB121588.1,
            AK054669.1, AK074161.1, DA436636.1, DA527125.1, DA013269.1,
            BC022100.1 and BC065365.1.
            
            On Nov 22, 2018 this sequence version replaced NM_080669.5.
            
            Summary: This gene encodes a transmembrane proton-coupled folate
            transporter protein that facilitates the movement of folate and
            antifolate substrates across cell membranes, optimally in acidic pH
            environments. This protein is also expressed in the brain and
            choroid plexus where it transports folates into the central nervous
            system. This protein further functions as a heme transporter in
            duodenal enterocytes, and potentially in other tissues like liver
            and kidney. Its localization to the apical membrane or cytoplasm of
            intestinal cells is modulated by dietary iron levels. Mutations in
            this gene are associated with autosomal recessive hereditary folate
            malabsorption disease. Alternatively spliced transcript variants
            encoding different isoforms have been described for this gene.
            [provided by RefSeq, Aug 2013].
            
            Transcript Variant: This variant (1, also known as HCP-1A) encodes
            the longer protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.160177.1,
                                           SRR1660807.211236.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000612814.5/ ENSP00000480703.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-534               DA188147.1         1-534
            535-688             DB121588.1         351-504
            689-1811            AK054669.1         687-1809
            1812-2405           AK074161.1         826-1419
            2406-2544           DA436636.1         300-438
            2545-2884           DA527125.1         41-380              c
            2885-3313           DA013269.1         147-575
            3314-4539           AK074161.1         2328-3553
            4540-5418           BC022100.1         226-1104
            5419-6113           BC065365.1         742-1436
            6114-6492           BC065365.1         1438-1816
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q11.2"
     gene            1..6492
                     /gene="SLC46A1"
                     /gene_synonym="G21; HCP1; hPCFT; HsPCFT; PCFT"
                     /note="solute carrier family 46 member 1"
                     /db_xref="GeneID:113235"
                     /db_xref="HGNC:HGNC:30521"
                     /db_xref="MIM:611672"
     exon            1..326
                     /gene="SLC46A1"
                     /gene_synonym="G21; HCP1; hPCFT; HsPCFT; PCFT"
                     /inference="alignment:Splign:2.1.0"
     CDS             99..1478
                     /gene="SLC46A1"
                     /gene_synonym="G21; HCP1; hPCFT; HsPCFT; PCFT"
                     /note="isoform 1 is encoded by transcript variant 1; heme
                     carrier protein 1; proton-coupled folate transporter;
                     solute carrier family 46 (folate transporter), member 1"
                     /codon_start=1
                     /product="proton-coupled folate transporter isoform 1"
                     /protein_id="NP_542400.2"
                     /db_xref="CCDS:CCDS74020.1"
                     /db_xref="GeneID:113235"
                     /db_xref="HGNC:HGNC:30521"
                     /db_xref="MIM:611672"
                     /translation="MEGSASPPEKPRARPAAAVLCRGPVEPLVFLANFALVLQGPLTT
                     QYLWHRFSADLGYNGTRQRGGCSNRSADPTMQEVETLTSHWTLYMNVGGFLVGLFSST
                     LLGAWSDSVGRRPLLVLASLGLLLQALVSVFVVQLQLHVGYFVLGRILCALLGDFGGL
                     LAASFASVADVSSSRSRTFRMALLEASIGVAGMLASLLGGHWLRAQGYANPFWLALAL
                     LIAMTLYAAFCFGETLKEPKSTRLFTFRHHRSIVQLYVAPAPEKSRKHLALYSLAIFV
                     VITVHFGAQDILTLYELSTPLCWDSKLIGYGSAAQHLPYLTSLLALKLLQYCLADAWV
                     AEIGLAFNILGMVVFAFATITPLMFTGYGLLFLSLVITPVIRAKLSKLVRETEQGALF
                     SAVACVNSLAMLTASGIFNSLYPATLNFMKGFPFLLGAGLLLIPAVLIGMLEKADPHL
                     EFQQFPQSP"
     exon            327..1179
                     /gene="SLC46A1"
                     /gene_synonym="G21; HCP1; hPCFT; HsPCFT; PCFT"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1263
                     /gene="SLC46A1"
                     /gene_synonym="G21; HCP1; hPCFT; HsPCFT; PCFT"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1420
                     /gene="SLC46A1"
                     /gene_synonym="G21; HCP1; hPCFT; HsPCFT; PCFT"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..6492
                     /gene="SLC46A1"
                     /gene_synonym="G21; HCP1; hPCFT; HsPCFT; PCFT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2074..2079
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC46A1"
                     /gene_synonym="G21; HCP1; hPCFT; HsPCFT; PCFT"
                     /note="hexamer: ATTACA"
     polyA_site      2101
                     /gene="SLC46A1"
                     /gene_synonym="G21; HCP1; hPCFT; HsPCFT; PCFT"
                     /note="major polyA site"
     regulatory      2878..2883
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC46A1"
                     /gene_synonym="G21; HCP1; hPCFT; HsPCFT; PCFT"
                     /note="hexamer: AATAAA"
     polyA_site      2903
                     /gene="SLC46A1"
                     /gene_synonym="G21; HCP1; hPCFT; HsPCFT; PCFT"
     regulatory      5394..5399
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC46A1"
                     /gene_synonym="G21; HCP1; hPCFT; HsPCFT; PCFT"
                     /note="hexamer: AATAAA"
     polyA_site      5418
                     /gene="SLC46A1"
                     /gene_synonym="G21; HCP1; hPCFT; HsPCFT; PCFT"
     polyA_site      5451
                     /gene="SLC46A1"
                     /gene_synonym="G21; HCP1; hPCFT; HsPCFT; PCFT"
     regulatory      6466..6471
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC46A1"
                     /gene_synonym="G21; HCP1; hPCFT; HsPCFT; PCFT"
                     /note="hexamer: AATAAA"
     polyA_site      6492
                     /gene="SLC46A1"
                     /gene_synonym="G21; HCP1; hPCFT; HsPCFT; PCFT"
ORIGIN      
        1 agacagcgca agccccacgc cacgcgtcgc tggtcccagg cagcgagtcg ctcgcgcgcc
       61 ccgccgcccg cctggcgaca gctccgccgc gcacgcacat ggaggggagc gcgagccccc
      121 cggaaaagcc ccgcgcccgc cctgcggctg ccgtgctgtg ccggggcccg gtagagccgc
      181 tggtcttcct ggccaacttt gccttggtcc tgcagggccc gctcaccacg cagtatctgt
      241 ggcaccgctt cagcgccgac ctcggctaca atggcacccg ccaaaggggg ggctgcagca
      301 accgcagcgc ggaccccacc atgcaggaag tggagaccct tacctcccac tggaccctct
      361 acatgaacgt gggcggcttc ctggtggggc tcttctcgtc caccctgctg ggagcttgga
      421 gcgacagtgt gggccgccgc ccgctgctag tgctggcctc gctgggcctg ctgctccagg
      481 ccctagtgtc cgtttttgtg gtgcagctgc agctccacgt cggctacttc gtgctgggtc
      541 gcatcctttg tgccctcctc ggcgacttcg gtggccttct ggctgctagc tttgcgtccg
      601 tggcagatgt cagctccagt cgcagccgca ccttccggat ggccctgctg gaagccagca
      661 tcggggtggc tgggatgctg gcaagcctcc tcgggggcca ctggctccgg gcccagggtt
      721 atgccaaccc cttctggctg gccttggcct tgctgatagc catgactctc tatgcagctt
      781 tctgctttgg tgagacctta aaggagccaa agtccacccg gctcttcacg ttccgtcacc
      841 accgatccat tgtccagctc tatgtggctc ccgccccaga gaagtccagg aaacatttag
      901 ccctctactc actggccatc ttcgtggtga tcactgtgca ctttggggcc caggacatct
      961 taacccttta tgaactaagc acacccctct gctgggactc caaactaatc ggctatggtt
     1021 ctgcagctca gcatctcccc tacctcacca gcctgctggc cctgaagctc ctgcagtact
     1081 gcctggccga tgcctgggta gctgagatcg gcctggcctt caacatcctg gggatggtgg
     1141 tctttgcctt tgccactatc acgcctctca tgttcacagg atatgggttg cttttcctgt
     1201 cattagtcat cacacctgtc atccgggcta aactctccaa gctggtgaga gagacagagc
     1261 agggtgctct cttttctgct gtggcctgtg tgaatagcct ggccatgctg acggcctccg
     1321 gcatcttcaa ctcactctac ccagccactc tgaactttat gaaggggttc cccttcctcc
     1381 tgggagctgg cctcctgctc atcccggctg ttctgattgg gatgctggaa aaggctgatc
     1441 ctcacctcga gttccagcag tttccccaga gcccctgatc tgcctggacc agaagacaga
     1501 gggcaagagg agcaaagtga acaccaagca actggaggtc tgcagctgga agcccagccc
     1561 acagcaggac aagcaactct tgtctaaggg cagtgctctc tttggacgag gtagtcaaga
     1621 gagaccaagg caccacccca tccacagctg acccagcctc tgtctaggat ctagaatcat
     1681 aacccacaca ggcccactgc aggacaggtg gcagaggagc tatttgggac aggagtcagt
     1741 tctccctttc tgctatccat cacttataac cccacaggcc agaggagagg tcctgagaga
     1801 ggtgacactt cagggaccag aggcagcacg agggctggca tctctccttc cagcccaaac
     1861 tgcacagccc caaccaggtt cccaacacgt gtagcagtca tcagccattc cttaacaatg
     1921 aatgtggtac ctggttagtg ccagccttgg gaaggaggga gggaggtaag aggggcttgg
     1981 tgatctctgg aggaagagtg ttgcctttgc tgtggttggg gaatcgatcc ttggctatgg
     2041 cctacccact cccctggctg aagagtgcca atcattacaa atcagcttca gcaaactgaa
     2101 acaaaccttc tggtgccttg ggctttgggg ccctctgttt tcctgctccc agtctgcctg
     2161 ccatacgtta gacaacagaa gctgctgggc tgggcaggag ccctgagctg cctggtggat
     2221 tggtaccaag tggggccagc cagggcttct actgactcgc tgtgcaacct tgagcaagcc
     2281 ccactaggaa cggctagatc agcaatgccc caaattgttg aatcgtggtg atcacatttg
     2341 gatggacaag ttaggaaact tagctgattc tctgagatac ttcccacttc ctcatacatt
     2401 ctacaatgag atgcctctct tcatgggcct ggggaaagga tgaatgagtg tggctggact
     2461 tttgaccttt aatgtccctg agcagttctt ggcccttggg gagaagtagg gggaggatct
     2521 ggctgactca gccccaaccg aggggtagca gctgcagccc agccaagcag aacaccctgg
     2581 ccctacagac ctgtgtctga gcaggctttg gaggaaaggc cacttctgag tcctctgtct
     2641 caggagaagc caggcctagg tgcttgacct ggtgatctga gctgtgtttg tcctagtatg
     2701 ggtggcagtc acctctgaac acactggcct tggcttagag gaggcttgtg acttcagctg
     2761 ttccagctgg ggctggatgc tggactctga ttccctcaga gagacagaag atcgggcaga
     2821 ggtcaaccag aaggccacac agggcagtgg acaacagtgg agggtatacc aaatagaaat
     2881 aaaacgcagg aaacctctct gaatactgcc cttggggacc tcaggaggga gtgggactga
     2941 ttggagacaa agagccagaa atcatcccct ggagatactc agacactgta ttgttattgg
     3001 cgctgtcctg tgagggcagt gtccaggctg ccctgcttgt catatccatg ccctggctct
     3061 ggccccggtg agcacttgtg ctgcatgagg aaaggaggat caggtggggt caaaaccaaa
     3121 gcatcgaggc tggcactggc caagacagac tctggcaggg aggggagtgg aaggaaggag
     3181 ccccaaacct ggagtgagac ctttgacttt ctgatgatgt ggccaagaac taggagtcac
     3241 ctggccagca cagccaagag tggggctggg ggcgcagcca gctttaaggt gtcaacacct
     3301 ggcccttgcg gggaaagagc cttcagcagc ccccacggcc tggagcctgg gtttattgga
     3361 gggtgctttc ttaccctctg aacccagtgg aggagggggc aatgggatat tcctgctctg
     3421 tatggcctct gggtcagctt tttgttgcaa aatcctatcc cccgctgcta gattctttga
     3481 agcaagggag aagcaatagc ccagcctcag aatgattctc aggaaagcaa ttccactgcc
     3541 agtggactgg gtgccaggag gcctaggtct ggatctgggg ctgccactaa tgacaggaaa
     3601 gtgggcttat cctcaacctt tctgagcctc agtgtcatta tctgtaaaat ggggatgata
     3661 ataccaggta gagttgctct gatgactgga gaccatggag cggagtgttt gggaaatggc
     3721 aaagatttgt gagggcttcg tgctttagag atttgctacc cttaaccacc agcatcagct
     3781 cacctgggaa tctgtaagaa atgcagagtc ctaggcccca ccccagatct cctgatttta
     3841 acaacattcc caggtgactt ggatgcatgt taaagttgga gaagctgccc taagtgacaa
     3901 ccctttgcct tttcaggact ttgcctcttt ccacagagct gcaggttctg tctaggctgc
     3961 ttttactgcc atgggggctg cctggtccct gagtaagcag tgggtagccc tggatggctg
     4021 aggggctggg gaagccagct gggccagacc aggaaggagc ctgaggcaca gcaccaaggc
     4081 atggggtagg gtgggctttg aggcacggcc tccccatgcc cagacccagg cccaaacagg
     4141 aggctcctgt gcaccagcaa gtgcagcaaa gctggctgca gtgacaagga agtcagaggg
     4201 agggctggtt tgcctcaacc ctcgccctgg atgtggcaaa agataccaca ccgaaggaag
     4261 ctgtagggga tctgggactt agtcaggcta gctaagggca ctgagtgatt acagggcaag
     4321 gccacagagc ggcaggaagt tgtaagtgca ggggccaggc acagtctgag tggagagcca
     4381 tgatggcact catccctggc tggctcctgg gcccaggccc ttgctcagaa tacagtgccc
     4441 aggcccgcct gggcattgcc ttaattcctg tctgaggctg ccttccgtca tcctcctcaa
     4501 gctgactgct atcagcagga gaagggaatg cagaaccacc cccaccccaa ctccagtgtc
     4561 tccagaactg agcagggacc ctccccatga cggagacaga caacccaatg gcagggcctg
     4621 ggggagttct aacctcccgt gcccaagcct gacttccctt ccctctccag atactgagga
     4681 aagtgggttg gaggtgggcc atgagggtgg gggacagggg gagggagagc catttcctaa
     4741 gaagagccca ggttgtctca gcccagggag actggtttca ggagctgttc cttcagaaag
     4801 gacgatggaa atggaagtca cagcagggct ggggaactgg ggactggtta ggttggaccc
     4861 atgggtgcag caccctccaa actggtgtca gagcctgcag atgagtcccg ggaggagcgg
     4921 ccctgcagga agcggatgat cttctcaatg gtggcctcct ggtattcgtg ggaccacctg
     4981 tgggcacagg agccaatgtg gttcagacag ctgggcagag ggagctgcta gtcagctgtt
     5041 tgcccacact ctgcctgtca tggtgatttg taccctaccc ccctggtccc agggccctgg
     5101 ggctcacttg ataccgttga aagtaagcac agcctgcatg tcctcaggca ggacctgggg
     5161 ctcaggccac tcgaagccat caatgatggg cacaatgttc ttgccgcagc ttaaagcagt
     5221 cacaatctcc tggagaaaga aaggaaagga ggaagatacc cctgtaccca tctagggacc
     5281 cttgtagcca ggcaggtgct gggaggccca ctggcccgag gctgggccca gggcaggaca
     5341 ccagtcctga tgtccacctt gatgtctacc ttaacccttg tagtgtaata aaaaataaat
     5401 atttttcctt tgcccagagt tcctggcaca gagctgctaa aacccttgga atctcttgag
     5461 tgatgagtgt ctttgtatgc taaagagatg attccccact gggggcccgg ggggtgctat
     5521 gggggtagcc tagatagttt caggaagagg gctggtcacc agggagacca gggcatgact
     5581 agaggattgg aactttcagc cccacctccc agcctccaga gaggtgctgg agattgagtt
     5641 caatcaccaa tggccaatga tttaatcaat catgcctaca taatggaacc tccataaaaa
     5701 cccccaaaca aggggttttg aagagcctcc aggttgataa acaggtgtag gtgctgggaa
     5761 ggtggtagaa ggcatggcat ccacacctcc accctccata cgtagagatc ctatgtatct
     5821 cttccatttg gctattcttg agctgtgtcc tttataactg tgaaattaaa taattcagct
     5881 gggcatggtg ggtcacgcct gtaatcccaa cactttggga ggctgaggca ggaggatcac
     5941 ttgagcccag gattttgaga ttaataatat tatttattac tattattcaa gctttgttaa
     6001 atcaaaccta aagctcttgg aactttaagt tattctgagc cttgacagga attggtctct
     6061 gcagcctgag tcatggggca tgcagctgca acttccacct attttttttt tttctgtaat
     6121 tacttaggaa gaccaaatgg caccagagat aagactccct tagatcccca cttagatcac
     6181 caccctttct cagggggtaa taaagtcatc ttccttggaa tgtagcaatc tataaccaat
     6241 caaagcactg taacatacgc actggtcttg tatggaaaat gttgtaatcc tgctaaaatt
     6301 cctctctctt tgcctgtgga agtgaaacct taacttctcc agtttggaat gctcacccca
     6361 tgcctttgga gtcaatgctt actgggtggc tattctcaaa ctttgcactc aaagaaactc
     6421 tatacttagt cttattttct gaatctcatt atttaaggtt gacataataa acaactaata
     6481 gtaagtaaag tg
//
