LOCUS       NM_152313               6037 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens solute carrier family 36 member 4 (SLC36A4),
            transcript variant 1, mRNA.
ACCESSION   NM_152313
VERSION     NM_152313.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Fan,S.J., Snell,C., Turley,H., Li,J.L., McCormick,R., Perera,S.M.,
            Heublein,S., Kazi,S., Azad,A., Wilson,C., Harris,A.L. and
            Goberdhan,D.C.
  TITLE     PAT4 levels control amino-acid sensitivity of rapamycin-resistant
            mTORC1 from the Golgi and affect clinical outcome in colorectal
            cancer
  JOURNAL   Oncogene 35 (23), 3004-3015 (2016)
   PUBMED   26434594
  REMARK    GeneRIF: Data predict that colorectal cancer cells with high PAT4
            expression will be more resistant to depletion of serine and
            glutamine, allowing them to survive and outgrow neighbouring normal
            and tumorigenic cells.
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Kerns,S.L., Stock,R.G., Stone,N.N., Blacksburg,S.R., Rath,L.,
            Vega,A., Fachal,L., Gomez-Caamano,A., De Ruysscher,D.,
            Lammering,G., Parliament,M., Blackshaw,M., Sia,M., Cesaretti,J.,
            Terk,M., Hixson,R., Rosenstein,B.S. and Ostrer,H.
  TITLE     Genome-wide association study identifies a region on chromosome
            11q14.3 associated with late rectal bleeding following radiation
            therapy for prostate cancer
  JOURNAL   Radiother Oncol 107 (3), 372-376 (2013)
   PUBMED   23719583
REFERENCE   3  (bases 1 to 6037)
  AUTHORS   Schioth,H.B., Roshanbin,S., Hagglund,M.G. and Fredriksson,R.
  TITLE     Evolutionary origin of amino acid transporter families SLC32, SLC36
            and SLC38 and physiological, pathological and therapeutic aspects
  JOURNAL   Mol Aspects Med 34 (2-3), 571-585 (2013)
   PUBMED   23506890
  REMARK    Review article
REFERENCE   4  (bases 1 to 6037)
  AUTHORS   Comuzzie,A.G., Cole,S.A., Laston,S.L., Voruganti,V.S., Haack,K.,
            Gibbs,R.A. and Butte,N.F.
  TITLE     Novel genetic loci identified for the pathophysiology of childhood
            obesity in the Hispanic population
  JOURNAL   PLoS One 7 (12), e51954 (2012)
   PUBMED   23251661
REFERENCE   5  (bases 1 to 6037)
  AUTHORS   Pillai,S.M. and Meredith,D.
  TITLE     SLC36A4 (hPAT4) is a high affinity amino acid transporter when
            expressed in Xenopus laevis oocytes
  JOURNAL   J Biol Chem 286 (4), 2455-2460 (2011)
   PUBMED   21097500
  REMARK    GeneRIF: SLC36A4 (hPAT4) is a high affinity amino acid transporter
            when expressed in Xenopus laevis oocytes.
REFERENCE   6  (bases 1 to 6037)
  AUTHORS   Heublein,S., Kazi,S., Ogmundsdottir,M.H., Attwood,E.V., Kala,S.,
            Boyd,C.A., Wilson,C. and Goberdhan,D.C.
  TITLE     Proton-assisted amino-acid transporters are conserved regulators of
            proliferation and amino-acid-dependent mTORC1 activation
  JOURNAL   Oncogene 29 (28), 4068-4079 (2010)
   PUBMED   20498635
  REMARK    GeneRIF: SLC36A4 has a similar in vivo growth regulatory activity
            to fly SLC36 family members when expressed in Drosophila and is
            also required for amino acid-dependent mTORC1 activation, and
            mTORC1-regulated proliferation in human cells.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP003072.2, HY170772.1 and AY162216.1.
            
            On Nov 22, 2018 this sequence version replaced NM_152313.3.
            
            Summary: SLC36A4 belongs to the SLC36 family of amino acid
            transporters based on sequence similarity with other family members
            (e.g., SLC36A1; MIM 606561). SLC36 proteins contain about 500 amino
            acids and have 9 to 11 transmembrane domains. Unlike other SLC36
            family members, which are proton-coupled amino acid transporters,
            SLC36A4 is a high-affinity/low-capacity non-proton-coupled amino
            acid transporter (Pillai and Meredith, 2011 [PubMed
            21097500]).[supplied by OMIM, Feb 2011].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.230169.1,
                                           SRR1660803.15402.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2159080 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000326402.9/ ENSP00000317382.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AP003072.2         69884-69899         c
            17-62               HY170772.1         1-46
            63-1846             AY162216.1         1-1784
            1847-6037           AP003072.2         16082-20272         c
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q21"
     gene            1..6037
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /note="solute carrier family 36 member 4"
                     /db_xref="GeneID:120103"
                     /db_xref="HGNC:HGNC:19660"
                     /db_xref="MIM:613760"
     exon            1..214
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /inference="alignment:Splign:2.1.0"
     CDS             160..1674
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     proton-coupled amino acid transporter 4; solute carrier
                     family 36 (proton/amino acid symporter), member 4; neutral
                     amino acid uniporter 4"
                     /codon_start=1
                     /product="neutral amino acid uniporter 4 isoform 1"
                     /protein_id="NP_689526.2"
                     /db_xref="CCDS:CCDS8291.1"
                     /db_xref="GeneID:120103"
                     /db_xref="HGNC:HGNC:19660"
                     /db_xref="MIM:613760"
                     /translation="MEAAATPAAAGAARREELDMDVMRPLINEQNFDGTSDEEHEQEL
                     LPVQKHYQLDDQEGISFVQTLMHLLKGNIGTGLLGLPLAIKNAGIVLGPISLVFIGII
                     SVHCMHILVRCSHFLCLRFKKSTLGYSDTVSFAMEVSPWSCLQKQAAWGRSVVDFFLV
                     ITQLGFCSVYIVFLAENVKQVHEGFLESKVFISNSTNSSNPCERRSVDLRIYMLCFLP
                     FIILLVFIRELKNLFVLSFLANVSMAVSLVIIYQYVVRNMPDPHNLPIVAGWKKYPLF
                     FGTAVFAFEGIGVVLPLENQMKESKRFPQALNIGMGIVTTLYVTLATLGYMCFHDEIK
                     GSITLNLPQDVWLYQSVKILYSFGIFVTYSIQFYVPAEIIIPGITSKFHTKWKQICEF
                     GIRSFLVSITCAGAILIPRLDIVISFVGAVSSSTLALILPPLVEILTFSKEHYNIWMV
                     LKNISIAFTGVVGFLLGTYITVEEIIYPTPKVVAGTPQSPFLNLNSTCLTSGLK"
     exon            215..338
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            339..429
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            430..518
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            519..614
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            615..699
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            700..927
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1026
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1196
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1366
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..6037
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2507..2512
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /note="hexamer: AATAAA"
     polyA_site      2527
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /note="major polyA site"
     regulatory      6018..6023
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /note="hexamer: AATAAA"
     polyA_site      6037
                     /gene="SLC36A4"
                     /gene_synonym="PAT4"
                     /note="major polyA site"
ORIGIN      
        1 gcatgctcct cgccgcgcgg ttggggagcg ccggggcagg gaggcgggca ccaggcgcgg
       61 gtccctccgg gcaggggagg taggcctggg cctgacgccg gccacgcagc ggcgggagag
      121 tgagcactcg ggcggcggcg tcctggagac ccgcgagaga tggaagcggc ggcgacgccg
      181 gcggctgccg gggcggcgag gcgcgaggag ctagatatgg atgtaatgag gcccttgata
      241 aatgagcaga attttgatgg gacatcagat gaagaacatg agcaagagct tctgcctgtt
      301 cagaagcatt accaacttga tgatcaagag ggcatttcat ttgtacaaac tcttatgcac
      361 cttcttaaag gaaatattgg aactggcctt ttaggacttc cattggcaat aaaaaatgca
      421 ggcatagtgc ttggaccaat cagccttgtg tttataggaa ttatttctgt tcactgtatg
      481 cacatattgg tacgttgcag tcactttcta tgtctgaggt ttaaaaagtc aacattaggt
      541 tatagtgaca ctgtgagctt tgctatggaa gtgagtcctt ggagttgtct tcagaagcaa
      601 gcagcatggg ggcggagtgt ggttgacttt tttctggtga taacacagct gggattctgt
      661 agtgtttata ttgtcttctt agctgaaaat gtgaaacaag ttcatgaagg attcctggag
      721 agtaaagtgt ttatttcaaa tagtaccaat tcatcaaacc cttgtgagag aagaagtgtt
      781 gacctaagga tatatatgct ttgctttctt ccatttataa ttcttttggt cttcattcgt
      841 gaactaaaga atctatttgt actttcattc cttgccaacg tttccatggc tgtcagtctt
      901 gtgataattt accagtatgt tgtcaggaac atgccagatc cccacaacct tccaatagtg
      961 gctggttgga agaaataccc actctttttt ggtactgctg tatttgcttt tgaaggcata
     1021 ggagtggtcc ttccactgga aaaccaaatg aaagaatcaa agcgtttccc tcaagcgttg
     1081 aatattggca tggggattgt tacaactttg tatgtaacat tagctacttt aggatatatg
     1141 tgtttccatg atgaaatcaa aggcagcata actttaaatc ttccccaaga tgtatggtta
     1201 tatcaatcag tgaaaattct atattccttt ggcatttttg tgacatattc aattcagttc
     1261 tatgttccag cagagatcat tatccctggg atcacatcca aatttcatac taaatggaag
     1321 caaatctgtg aatttgggat aagatccttc ttggttagta ttacttgtgc cggagcaatt
     1381 cttattcctc gtttagacat tgtgatttcc ttcgttggag ctgtgagcag cagcacattg
     1441 gccctaatcc tgccaccttt ggttgaaatt cttacatttt cgaaggaaca ttataatata
     1501 tggatggtcc tgaaaaatat ttctatagca ttcactggag ttgttggctt cttattaggt
     1561 acatatataa ctgttgaaga aattatttat cctactccca aagttgtagc tggcactcca
     1621 cagagtcctt ttctaaattt gaattcaaca tgcttaacat ctggtttgaa atagtaaaag
     1681 cagaatcatg agtcttctat ttttgtccca tttctgaaaa ttatcaagat aactagtaaa
     1741 atacattgct atatacataa aaatggtaac aaactctgtt ttctttggca cgatattaat
     1801 attttggaag taatcataac tctttaccag tagtggtaaa cctatgaaaa atccttgctt
     1861 ttaagtgtta gcaatagttc aaaaaattaa gttctgaaaa ttgaaaaaat taaaatgtaa
     1921 aaaaattaaa gaataaaaat acttctatta ttcttttatc tcagtaagaa ataccttaac
     1981 caagttatct ctcttttatg ctactctttt gccactcact tgagaacaga ataggatttc
     2041 aacaataaga gaataaaata agaacatgta taacaaaaag ctctctccag atcatccctg
     2101 tgaatgccaa agtaaacttt atgtacagtg taaaaaaaaa aaatctcagt tatgttttta
     2161 ttagccaaat tctaatgatt ggctcctgga agtatagaaa actcccatta acataatata
     2221 agcatcagaa aattgcaaac actagaatta attttacact ctaatggtag ttgatcttca
     2281 tagtcaagag gcactgttca agatcatgac ttagtgtttc aatgaaattt gaaaagggac
     2341 tttaaaactt atccagtgca actcccttgt ttttcgtcag aggaaaagga ggcctagaaa
     2401 ggttaagtaa cttggtcgag accactcagc cttgagatca agaaaaccta atcttctgac
     2461 tcccaggcca ggatgtttta tttctcacat catgtccaag aaaaagaata aattatgttc
     2521 agcttaattt tagtgttgaa tctatttgat tatattttaa tactttgaaa atgaatgtgt
     2581 gatttttaat agtatatgtg acctgagcag aaaatcaggg aactccaaga agcctacact
     2641 gtggccatat aaacctcagc aagagaaaga agctatgttc ttttaaaaca gaatagagac
     2701 cgcttgctgg tgaaactcct ggctagtaag atgtgtgtct agctatacta tttgtggctt
     2761 gagctttttt aattattacc ttcctttcct gagttttgta ggcaccacat tcctgaatgg
     2821 cagaaaatag acacctcaga aaacggagga tttgtggact ctttccagcc ctgtggcttt
     2881 tcttatcaca gccttttatt tattatgagc agaataaaag aatcagctag gtgtggtggt
     2941 ctgtgcttat aatcccagct actctggagg ataagttggg aggatcactt gagaggccag
     3001 gagcttgaga ccagcctggg cagcatagtg agacctcgac tctataaaac ataaaaattt
     3061 aaaaaaaaat tagcctgatg tggcgtgtac ctgtagtccc agctactgag gaggctgaag
     3121 caggaggatc cctcgagcac aaaagtttca ggctgtggta agctatgact gtgccactgc
     3181 acaccagcct gagttacaga gggagatccc aactcttaaa aactaaaaca acaataaata
     3241 tatacaagaa tcataacata aagggattca tgcttagaaa aaatccataa actcccttct
     3301 aaatattgag acactccagg cttctttcag acaaataact tctaattatt ccatattttt
     3361 caagttatta accaagataa agaatctctc agttagtggg gaaaatgaaa attattaaga
     3421 atagaattgt cttctgactt taaaaacaat ttagacttta aaacatgaac tgtttactca
     3481 ggctggtgat actctagttt gttagtatac catacttgaa gatatcatca agatcactat
     3541 agttgtatat attctctatt tttatatgta aatgttaact tagttcaagt attttttgct
     3601 tgtatcgtta actgatcatc aaatacaatc ctaaagatat atcagaagct ttattttggt
     3661 acaaagtcat aagaatcaaa acttttttaa ccattcacat taggtatcaa cagtaattgt
     3721 ttgagatact tttatatcaa ttctgttaca ctgagccttt agtcatacta agagaatgca
     3781 gaagaagtta taggaaaacg aatcttcact gaaactagta ttatataatc ttgaattaga
     3841 tgtttaaaaa actttatagc ttgatataaa atgagttgaa aattattatt taataggaag
     3901 cagcattaga tttttgtcac ctgttttcag ataatatttc tagtctatgt atgttattta
     3961 atttttacac tttggcccat tgtttatttt ccttaaggaa ttcatcaaat gcaatgaaat
     4021 ttgaataaaa ttgatcatag caataaataa tttttaaatt cagcacaaac tcatcttgag
     4081 tatattatca atcaggcttc ctttagcaat aagaatggtg aatcaacatc cagtactaaa
     4141 ttcaataaat gatgaatact ttaaatacaa gaaaaagttg aattgcagaa aagaagaggg
     4201 gctaaaaagg aagaaagttt taattctcaa caagaaacac tatttagatg gaaagcttaa
     4261 atgtattctt attaagggta tttgatgatg cattttattt gcaaaaataa ccagtgggta
     4321 attttttctc tgaaaattta gctttaataa gtcgtaaaat ttcctaaaga ttttggagac
     4381 gatatttcga ttataccagc tcttgctttt attttgggag cctggagaaa tgagagtgat
     4441 tgcacctgtc aaattttgta tctcatcact tttggatcat cacactgtga ctaaaatgac
     4501 ttcagcattt tgttggccac cacagtgaag aactattccc tgaaagttaa tcagttgcct
     4561 gagagacttt tctgctactg aggggaaaga aaggaaagct ttatttacct ctaatgagct
     4621 ccgttccaag tcagttaatc actgttccct tacacccaag agtttcatca gtatcagagt
     4681 caacttgtca aaacactggt tctcagacgt ttttttcaga gggacttctt ttccccacat
     4741 ggccagattt ctttacaatt aagaaaataa gagctacagc gtattcttgg ttaaaaacca
     4801 gtttagatga tgttaccaaa tgaaaacaag tctgtattag caaatgccac attatattta
     4861 gtcgtattct cactaaattc tccgttatta aaatgatttc tgcatcttgc ctggaaaatg
     4921 cacttacata tttacaagcg ttgtatgtca atgtgcatgt tttgaatttc ctaacagtgt
     4981 ctaatcttag tagtttcata gtagttatgt aaaatctctt taactgcaaa atactataaa
     5041 atattacttg aaatattaaa gtattagtgc catttctagc tgtggcaagg cacaagtctt
     5101 actatagctt acttatgtct tctcatagat ttcagtacag catctgagta gtggatctgt
     5161 ttactgacag tatacacaaa ctaggtcttg acctcacttc tttcttcatt taaaaggaga
     5221 agatttgaaa tagagctagt gtaaataata aataaaatta tttaaatagc atcagtggct
     5281 caataccatc tgttgaggaa taaaataatt tggttaacat acagaacatc gataatatac
     5341 aataatgact ttttaaaaaa tgagtctgat ctttttggct cacctagctt cccttaataa
     5401 gaaaatttga gaaacttgga tctgaaaagt gtagttgtac ttcctaacat taaattcctg
     5461 gctgtaatag ttaatacttt ggcagcatta tggttagtct tgtttatgcc attggcactt
     5521 actaggctaa actttctgaa actgattggt tttgacctat gagataagga aacatgccta
     5581 agaaatggct agaaatgcta tttattgccc tatgataagt gaaatagcaa atcactcttg
     5641 attcttaaaa tttcatatta aaattagcta gttcctacat gcatgcgtgc acaagtatat
     5701 gcagacacac acacaaatca gtaatctatg tgaactagat actaatacta aattactatt
     5761 taaattgggt ccagaaagat gctgttttga aaagaacact aaaaatattt cattagcgtt
     5821 ttcttgataa caaataggaa aatatatgtt tacaccctga ataaattcag ctgtgggtgg
     5881 atttttgttt ttcaaaatga ttttgttagt ttgaatgaaa cctaaatata acattcaagt
     5941 caattctttt aacatttttc tttataacag aatatgaaaa acgctgtctt aaagtgttgt
     6001 aacttttctg aattttcaat aaaatatttt gcttgca
//
