LOCUS       NM_015139               6193 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens solute carrier family 35 member D1 (SLC35D1), mRNA.
ACCESSION   NM_015139
VERSION     NM_015139.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Ozer,L., Aktuna,S., Unsal,E., Unal,M.A., Sahin,G. and Baltaci,V.
  TITLE     A novel SLC35D1 variant causing milder phenotype of Schneckenbecken
            dysplasia in a large pedigree
  JOURNAL   Am J Med Genet A 188 (10), 3078-3083 (2022)
   PUBMED   35934917
  REMARK    GeneRIF: A novel SLC35D1 variant causing milder phenotype of
            Schneckenbecken dysplasia in a large pedigree.
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Rautengarten,C., Quarrell,O.W., Stals,K., Caswell,R.C., De
            Franco,E., Baple,E., Burgess,N., Jokhi,R., Heazlewood,J.L.,
            Offiah,A.C., Ebert,B. and Ellard,S.
  TITLE     A hypomorphic allele of SLC35D1 results in Schneckenbecken-like
            dysplasia
  JOURNAL   Hum Mol Genet 28 (21), 3543-3551 (2019)
   PUBMED   31423530
REFERENCE   3  (bases 1 to 6193)
  AUTHORS   Li,G.D., Wang,D., Zhang,D.F., Xiang,Q., Feng,J.Q., Li,X.A., Li,Y.Y.
            and Yao,Y.G.
  TITLE     Fine mapping of the GWAS loci identifies SLC35D1 and IL23R as
            potential risk genes for leprosy
  JOURNAL   J Dermatol Sci 84 (3), 322-329 (2016)
   PUBMED   27712858
  REMARK    GeneRIF: there is a positive association between the GWAS reported
            rs3762318 and leprosy, and SLC35D1 and IL23R might be the causal
            genes
REFERENCE   4  (bases 1 to 6193)
  AUTHORS   Kenny,E.E., Pe'er,I., Karban,A., Ozelius,L., Mitchell,A.A.,
            Ng,S.M., Erazo,M., Ostrer,H., Abraham,C., Abreu,M.T., Atzmon,G.,
            Barzilai,N., Brant,S.R., Bressman,S., Burns,E.R., Chowers,Y.,
            Clark,L.N., Darvasi,A., Doheny,D., Duerr,R.H., Eliakim,R.,
            Giladi,N., Gregersen,P.K., Hakonarson,H., Jones,M.R., Marder,K.,
            McGovern,D.P., Mulle,J., Orr-Urtreger,A., Proctor,D.D., Pulver,A.,
            Rotter,J.I., Silverberg,M.S., Ullman,T., Warren,S.T., Waterman,M.,
            Zhang,W., Bergman,A., Mayer,L., Katz,S., Desnick,R.J., Cho,J.H. and
            Peter,I.
  TITLE     A genome-wide scan of Ashkenazi Jewish Crohn's disease suggests
            novel susceptibility loci
  JOURNAL   PLoS Genet 8 (3), e1002559 (2012)
   PUBMED   22412388
REFERENCE   5  (bases 1 to 6193)
  AUTHORS   Furuichi,T., Kayserili,H., Hiraoka,S., Nishimura,G., Ohashi,H.,
            Alanay,Y., Lerena,J.C., Aslanger,A.D., Koseki,H., Cohn,D.H.,
            Superti-Furga,A., Unger,S. and Ikegawa,S.
  TITLE     Identification of loss-of-function mutations of SLC35D1 in patients
            with Schneckenbecken dysplasia, but not with other severe
            spondylodysplastic dysplasias group diseases
  JOURNAL   J Med Genet 46 (8), 562-568 (2009)
   PUBMED   19508970
  REMARK    GeneRIF: Searched for SLC35D1 mutations, identified four novel
            mutations in three Schneckenbecken dysplasia families. All
            mutations result in loss of function. No SLC35D1 mutations were
            id'd in all patients with other spondylodysplastic dysplasias group
            diseases.
REFERENCE   6  (bases 1 to 6193)
  AUTHORS   Hiraoka,S., Furuichi,T., Nishimura,G., Shibata,S., Yanagishita,M.,
            Rimoin,D.L., Superti-Furga,A., Nikkels,P.G., Ogawa,M.,
            Katsuyama,K., Toyoda,H., Kinoshita-Toyoda,A., Ishida,N., Isono,K.,
            Sanai,Y., Cohn,D.H., Koseki,H. and Ikegawa,S.
  TITLE     Nucleotide-sugar transporter SLC35D1 is critical to chondroitin
            sulfate synthesis in cartilage and skeletal development in mouse
            and human
  JOURNAL   Nat Med 13 (11), 1363-1367 (2007)
   PUBMED   17952091
  REMARK    GeneRIF: Loss of function mutations cause Schneckenbecken
            dysplasia, a severe skeletal dysplasia.
REFERENCE   7  (bases 1 to 6193)
  AUTHORS   Iyanagi,T.
  TITLE     Molecular mechanism of phase I and phase II drug-metabolizing
            enzymes: implications for detoxification
  JOURNAL   Int Rev Cytol 260, 35-112 (2007)
   PUBMED   17482904
  REMARK    Review article
REFERENCE   8  (bases 1 to 6193)
  AUTHORS   Wistow,G., Bernstein,S.L., Wyatt,M.K., Fariss,R.N., Behal,A.,
            Touchman,J.W., Bouffard,G., Smith,D. and Peterson,K.
  TITLE     Expressed sequence tag analysis of human RPE/choroid for the
            NEIBank Project: over 6000 non-redundant transcripts, novel genes
            and splice variants
  JOURNAL   Mol Vis 8, 205-220 (2002)
   PUBMED   12107410
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 6193)
  AUTHORS   Muraoka,M., Kawakita,M. and Ishida,N.
  TITLE     Molecular characterization of human UDP-glucuronic
            acid/UDP-N-acetylgalactosamine transporter, a novel nucleotide
            sugar transporter with dual substrate specificity
  JOURNAL   FEBS Lett 495 (1-2), 87-93 (2001)
   PUBMED   11322953
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK289800.1, D87449.1,
            BQ001392.1, CA391890.1, BU679620.1 and BU619019.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_015139.2.
            
            Summary: Glycosylation of cellular glycoconjugates occurs in the
            endoplasmic reticulum (ER) and Golgi compartment, and requires
            transport of nucleotide sugars from the cytosol into the lumen of
            the ER and Golgi by specific transporters. The protein encoded by
            this gene resides in the ER, and transports both UDP-glucuronic
            acid (UDP-GlcA) and UDP-N-acetylgalactosamine (UDP-GalNAc) from the
            cytoplasm to the ER lumen. It may participate in glucuronidation
            and/or chondroitin sulfate biosynthesis. Mutations in this gene are
            associated with Schneckenbecken dysplasia.[provided by RefSeq, Sep
            2009].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.12470.1,
                                           SRR1660809.38849.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000235345.6/ ENSP00000235345.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1547              AK289800.1         248-1794
            1548-5230           D87449.1           1499-5181
            5231-5256           BQ001392.1         1-26                c
            5257-5371           CA391890.1         331-445
            5372-5432           BU679620.1         300-360
            5433-6161           D87449.1           5190-5918
            6162-6193           BU619019.1         34-65               c
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p31.3"
     gene            1..6193
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /note="solute carrier family 35 member D1"
                     /db_xref="GeneID:23169"
                     /db_xref="HGNC:HGNC:20800"
                     /db_xref="MIM:610804"
     exon            1..338
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /inference="alignment:Splign:2.1.0"
     CDS             136..1203
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /note="UDP-glucuronic acid/UDP-N-acetylgalactosamine
                     transporter; UDP-galactose transporter-related 7;
                     UDP-GlcA/UDP-GalNAc transporter; solute carrier family 35
                     (UDP-GlcA/UDP-GalNAc transporter), member D1; solute
                     carrier family 35 (UDP-glucuronic
                     acid/UDP-N-acetylgalactosamine dual transporter), member
                     D1; UDP-galactose transporter-related protein 7"
                     /codon_start=1
                     /product="nucleotide sugar transporter SLC35D1"
                     /protein_id="NP_055954.1"
                     /db_xref="CCDS:CCDS636.1"
                     /db_xref="GeneID:23169"
                     /db_xref="HGNC:HGNC:20800"
                     /db_xref="MIM:610804"
                     /translation="MAEVHRRQHARVKGEAPAKSSTLRDEEELGMASAETLTVFLKLL
                     AAGFYGVSSFLIVVVNKSVLTNYRFPSSLCVGLGQMVATVAVLWVGKALRVVKFPDLD
                     RNVPRKTFPLPLLYFGNQITGLFSTKKLNLPMFTVLRRFSILFTMFAEGVLLKKTFSW
                     GIKMTVFAMIIGAFVAASSDLAFDLEGYAFILINDVLTAANGAYVKQKLDSKELGKYG
                     LLYYNALFMILPTLAIAYFTGDAQKAVEFEGWADTLFLLQFTLSCVMGFILMYATVLC
                     TQYNSALTTTIVGCIKNILITYIGMVFGGDYIFTWTNFIGLNISIAGSLVYSYITFTE
                     EQLSKQSEANNKLDIKGKGAV"
     exon            339..372
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /inference="alignment:Splign:2.1.0"
     exon            373..459
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /inference="alignment:Splign:2.1.0"
     exon            460..527
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /inference="alignment:Splign:2.1.0"
     exon            528..599
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /inference="alignment:Splign:2.1.0"
     exon            600..668
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /inference="alignment:Splign:2.1.0"
     exon            669..771
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /inference="alignment:Splign:2.1.0"
     exon            772..864
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /inference="alignment:Splign:2.1.0"
     exon            865..932
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1011
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1094
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..6193
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6144..6149
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /note="hexamer: AATAAA"
     polyA_site      6165
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /note="major polyA site"
     regulatory      6171..6176
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
                     /note="hexamer: ATTAAA"
     polyA_site      6193
                     /gene="SLC35D1"
                     /gene_synonym="SHNKND; UGTREL7"
ORIGIN      
        1 aagcggggag tcgaggcggc gagcgcgcag ctggcctagt ctgcggtccc caactcctgg
       61 agtcccctgg gagctgccgc tgcaggcccc cgagccgcta ggccccgccg ccaggccacc
      121 gctgctgcgg cagccatggc ggaagttcat agacgtcagc atgctcgggt taaaggagaa
      181 gcccccgcga aatcctccac actccgagat gaggaggagc tggggatggc gtcggccgaa
      241 acgctgaccg tgtttctgaa gctgctggcc gccggctttt acggcgtgag ctccttcctg
      301 atcgtggtgg tgaataagag cgtgctcacc aattacagat ttccctcctc actatgtgtt
      361 ggacttggcc agatggtggc cacagtggca gttctctggg tgggaaaggc gctcagagta
      421 gtcaagtttc ctgaccttga cagaaatgta cctcgaaaga cgtttccact acctctacta
      481 tattttggga accaaatcac gggactgttc agcacaaaga aactgaactt gccaatgttt
      541 acagttctga gaaggttctc catcctgttt acaatgtttg ctgaaggagt tttactcaag
      601 aagacttttt cttggggtat taaaatgact gtatttgcaa tgattattgg agcctttgta
      661 gctgccagct ctgacttggc atttgatctg gaaggatatg cttttattct gataaacgat
      721 gtcctaacag cagcaaatgg tgcatacgta aaacaaaaat tagattcaaa agagctggga
      781 aaatatggac tgctctatta caatgcactg ttcatgattc tgcccaccct ggccattgcg
      841 tatttcacag gagatgcaca aaaggctgtg gagtttgaag gctgggctga caccctcttt
      901 cttctgcagt tcaccctctc ctgtgtgatg gggtttatct taatgtacgc cacagtactc
      961 tgcacgcagt ataattctgc tcttacaact acaatagttg gctgtattaa gaatatatta
     1021 ataacttata ttggaatggt ctttggtgga gattatattt tcacgtggac aaacttcatt
     1081 ggtttaaata tcagcattgc tgggagcctg gtatattcct atatcacttt cactgaagag
     1141 cagctgagca aacagtcaga ggctaataac aagctggaca ttaaggggaa aggagcagtg
     1201 tgaccagagg attgcttcat ctgatacgta ggcctaagtt tttaatcaac tcagaacact
     1261 ggcagttcct acagagacac tgaggtgtct tgattatgga ggaaataacc attcctttgc
     1321 acttgtacaa tctgaggatt gattgctgcc ttttaaaatt ttatgaagag agaaacttat
     1381 aattgactct attttaaata tgttgtttga attaatttat atcagactgg aaaaggtact
     1441 gggcttttta atttattttt cttatatgcc gacagtgaaa catcagtgtt ttgaaagtat
     1501 tttatttcat aatttgatat ccaggtgtcc ctgagagctg catatgtaat gtttgccaac
     1561 tggagcttca tcattcaaat tgttgctgca tctggaggca gtttagcctt tcctctgctg
     1621 gaaatgcaga cgaatatgtg aaatttctct tctttgaatc ttatttctta tgattgcatt
     1681 gtcttcatca tatcatcatt ccatttgcca aggaaaacaa ctttggcact ataaaaatat
     1741 gtggggcttt gaagtgtatt gctatctatg ttggcttccc tgactttcat aggatgagca
     1801 gagtgcattc cttgaaaaca tgttcttttt ttgttgtgta gaatggggat aatgaagggg
     1861 gtgagaaagt aggcctttta ctaatttttc atctatttga cttattgatt caactaaaat
     1921 gttttaagta tatgtgccag gcattgtgct aaatgctggg gatacagaga tgtttgcaga
     1981 ggtagttgaa acatctcact cctgccatag tgactcaggt aaggttgaga atttagctct
     2041 gttaatctat tcagaagatg tcactaagag taagttgttt cagcactcaa aagccgcaaa
     2101 tctgtaaatt ggattaagaa cacctgccct acctacccgg caatagtgtg aatccaaatg
     2161 ctgcaatgtg agatgaggta caagtaaaga gctgtgtttt gtaaaccagt gcagctcagc
     2221 attgactccc tctctaagtg aaaattgcag acaggcaagt ctgttttccc ttgccacctt
     2281 cctttgggtt tggttgctct ttggatgaat atgatgaggt cccacatttc tgaatttatt
     2341 ggtttgaggg gaaaggctgg ttagacgtct ttgtattgtc aatggttggt ttttctctgg
     2401 cagtgtcagt gttagagcta tcatcttcct tcttaaatgg catgagcaat gagcaaactt
     2461 ccctcctttc aacattctca cctacatcat gtgccccttg gctcacctac tgagcttgcc
     2521 aacttctctc aaaaccaatg ctgcattttg ggagagcagc tgtgaatggg cactgcctaa
     2581 attgcctctt ggggtaatct ggagggtgga ggtgttaact caaacataac cctatgactt
     2641 gactcagaga tatccattga ctttgaggat tagtgtgttt gctcaggcca gttgtacagc
     2701 cagtgtgcaa gagatttggt acctttgcac taatgcacac accagtgtct atttcattgg
     2761 gtttggaatc cactggactc tgttgaccta aacagttagg gttttgattt tttttgatat
     2821 gcaaatttat attgagatca ttttcatatt cctgtctttt ggtttgtctt tactaaagct
     2881 tggctggtgg tgctgtgtcc ctagaattat ccctgcaacc aactcccaag ttacaagctt
     2941 cattaagagc cttagactca cttttttcta gcatcttctg acctgagaaa attccactgg
     3001 gagtaaagga tagtatcttg tgtcatatgg gtcttactct ccctatttta ccaatgagaa
     3061 aacaaaactc agagaaagat gacttgctaa tacatcaaac ccatgtcctt tttattgaac
     3121 tatgctgcca tttaattaac cgcccttccc ctctgaggga gtaaggaaac agatcctgag
     3181 ttcacttgag cactttcaaa aaagcaagca gttgattgaa tttggtgtca gagaggagca
     3241 tactttgcaa atcaggaaga gtgacagctt accctgtcct ggaaccctta tcccacttcc
     3301 aggctgactt tagttgataa tgcacttctg gctgtaaatg ttgccaacac ttcctggttt
     3361 tgcttaggta cagtgaaagg gcatagtgag agctattttt agtgattctg ttctttctgt
     3421 cagcaacatg aaggagggac gtttgaataa tttttgttgt agaatatttc ttcatgaaag
     3481 gcttttgggt gtctttaaca ggtgttgggc tagagcccca ccaggagttg aactgcctct
     3541 tgggggtcag gttccctagg agatctgtca cctcttcagg agtatttgag aacattgtgt
     3601 agttacagct tcactaaatg cacgtgacag gcttctgtgc cttgggagcc ctggcttatg
     3661 ctgaaacttc tggctgacca gagcagtaca gcaactttgc cctgtctgtg ttctgcttcc
     3721 tggatgcatc cttcagccac taaccagcat tgcaagatgg agcagggtga acaagctgga
     3781 gactgaatag tcttttggag tttgcgtttt gattattgca ggcgtgttac aatatactgg
     3841 tgatttacct ggtactgtgg cacctcccag aaagtctggt ttttgcccca tgtttgatat
     3901 ttactaggca gaaattcttt gtcctgttaa ctgcctcctg ggagaaaaag aggcaataca
     3961 gagttaaatc cttaatttgc ccactgagac agatgagctg tggtattcca aaagacaaat
     4021 tctatgacta gtgtctcctg ttcaatgtaa gtgagaccct ctttggccaa tctgcttcta
     4081 gtcaaggcta aatggattgg aataacccta gttggccagg ctacacattg ccagcatgtc
     4141 ccgttgagtt ttaattgcct catgtggctg tggactttat gagtcaatca tttgtaggct
     4201 ccagtttgca cctagttttt ttttttaaaa agtgcattta cacgcttttg gtagtcctgt
     4261 tttatcaaga atccatggag ggttttggtc agtacagctg gtgcaggatc ctcacggaga
     4321 aacagtggag tttcacattg agttggtttg aaatggcacc ccaggacttt gggcctgcct
     4381 tacttgatag cctcgttcag tgagcaaaga cttagtgagc agctcttgta tgccaagtat
     4441 tttgctaagc tctggaaaaa agataaacaa gacatggttc ttgctttcaa ggagtgtgta
     4501 attctttagc cagatatgga aacctggacc ctgagtggga gaaaggagac agatgaaagg
     4561 agtccgtgat tttgtaacca agagctgcct gcatggttat gagtatcact gattttaggg
     4621 acgccccaca gagctaaagc atttttttaa tccgagagga cttttgtaac tcatattagt
     4681 taatcttcta gctctgagat agcaacacag ctcttagaat tctgtaagta gcttgaggcc
     4741 tcctcctcta ctgtacatgt tcttgctttc ccaaaattgt aagtttggtt tttcctatga
     4801 acaagactgt aattgatttt tctctccctg aggaaaaaag ttcggaataa atgagacatg
     4861 gatatgaaac tacttagcac aatgcctggt acatattagg ccctcagcaa atgtggctac
     4921 cgtgtcatta tttcatggtg agactcaagg caaaacaaga ttcttaattt atgctccgta
     4981 aatgctagag gcttgggcaa tacggtctgt cctgaaaaac aggactctgt gtctcattgc
     5041 ctctagaatc cttaggtagc agtttctgtt cactgtcttt tcttttttaa aaatatctgc
     5101 tggacacaat tacggccatt acatgatgca ggtgattcta cctatttttt aaaaagaaca
     5161 aaatttcttt tatattgtga cagtacattt taataaagga aaattatttt cttcccccta
     5221 ctccccctcc ttttttttaa aaaaaaaaaa aaaaaagaaa gaaggtcttg ctctgtcgcc
     5281 agccaggctg gagtgtaatg gcgtaatcat agcagctcac cacagtctca aactcctagg
     5341 ctcaagtgat cctcctgcct tagcctgcta agtagctacg actataggca tgtgccatta
     5401 cgcctggctc gtttcttcct tttttttttt ttttctggta aagacagggt ctcactctgt
     5461 tgccagactg gtctcaaact cctggcctca agtaaccctt ctgtctcagc ctcccaaagt
     5521 gctgggatta caggtgtgtg ccacatgtcc ggccaggaaa attattttct atcttaaaga
     5581 aacatagtta cattttagaa tgtcaattca gttaaatctt tctcagtttt tacctttaga
     5641 tggctcctgg aaaggccagc gttccttttc ccctctctga attgaaagca cttgacccca
     5701 tgctgcccct gcttccaggc ctgtctcagt tgtcatatat tgtgagtccc tggcaccatt
     5761 actgttttct aagccagtct tctaggtctt actttcagga tgctgcttca tggcatgagg
     5821 atatagttta cagtgttgta gttttttttt ttttaaagct ttgttcttta aaaatacttt
     5881 ttaaaaagaa atctactggt tagatttatt gtctcacaaa aatgttttta gagattattt
     5941 ttttcacaaa tgttttcttt ctcatgctga tgatcaactg atttttgtct acaggtgtta
     6001 gcttctcaca gtattttttc attttagagt ctatttacca aatgaccatt tttagtgtta
     6061 aaaggtactg aggaattgag gtatgtaaat caggtgacat aaacatgaaa attatgtgcc
     6121 taaatatttt tgtaatggtg taaaataaat actttttcct gaaaacaagt attaaaattc
     6181 ctttgtaact gca
//
