LOCUS       NM_012243              14321 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens solute carrier family 35 member A3 (SLC35A3),
            transcript variant 1, mRNA.
ACCESSION   NM_012243
VERSION     NM_012243.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14321)
  AUTHORS   Lu,S., Sun,X., Tang,H., Yu,J., Wang,B., Xiao,R., Qu,J., Sun,F.,
            Deng,Z., Li,C., Yang,P., Yang,Z. and Rao,B.
  TITLE     Colorectal cancer with low SLC35A3 is associated with immune
            infiltrates and poor prognosis
  JOURNAL   Sci Rep 14 (1), 329 (2024)
   PUBMED   38172565
  REMARK    GeneRIF: Colorectal cancer with low SLC35A3 is associated with
            immune infiltrates and poor prognosis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14321)
  AUTHORS   Song,W., Isaji,T., Nakano,M., Liang,C., Fukuda,T. and Gu,J.
  TITLE     O-GlcNAcylation regulates beta1,4-GlcNAc-branched N-glycan
            biosynthesis via the OGT/SLC35A3/GnT-IV axis
  JOURNAL   FASEB J 36 (2), e22149 (2022)
   PUBMED   34981577
REFERENCE   3  (bases 1 to 14321)
  AUTHORS   Markolin,P., Davidson,N., Hirt,C.K., Chabbert,C.D., Zamboni,N.,
            Schwank,G., Krek,W. and Ratsch,G.
  TITLE     Identification of HIF-dependent alternative splicing in
            gastrointestinal cancers and characterization of a long, coding
            isoform of SLC35A3
  JOURNAL   Genomics 113 (2), 515-529 (2021)
   PUBMED   33418078
  REMARK    GeneRIF: Identification of HIF-dependent alternative splicing in
            gastrointestinal cancers and characterization of a long, coding
            isoform of SLC35A3.
REFERENCE   4  (bases 1 to 14321)
  AUTHORS   Szulc,B., Sosicka,P., Maszczak-Seneczko,D., Skurska,E.,
            Shauchuk,A., Olczak,T., Freeze,H.H. and Olczak,M.
  TITLE     Biosynthesis of GlcNAc-rich N- and O-glycans in the Golgi apparatus
            does not require the nucleotide sugar transporter SLC35A3
  JOURNAL   J Biol Chem 295 (48), 16445-16463 (2020)
   PUBMED   32938718
  REMARK    GeneRIF: Biosynthesis of GlcNAc-rich N- and O-glycans in the Golgi
            apparatus does not require the nucleotide sugar transporter
            SLC35A3.
REFERENCE   5  (bases 1 to 14321)
  AUTHORS   Haouari,W., Dubail,J., Lounis-Ouaras,S., Prada,P., Bennani,R.,
            Roseau,C., Huber,C., Afenjar,A., Colin,E., Vuillaumier-Barrot,S.,
            Seta,N., Foulquier,F., Pous,C., Cormier-Daire,V. and Bruneel,A.
  TITLE     Serum bikunin isoforms in congenital disorders of glycosylation and
            linkeropathies
  JOURNAL   J Inherit Metab Dis 43 (6), 1349-1359 (2020)
   PUBMED   32700771
  REMARK    GeneRIF: Serum bikunin isoforms in congenital disorders of
            glycosylation and linkeropathies.
            Erratum:[J Inherit Metab Dis. 2022 Nov;45(6):1206. doi:
            10.1002/jimd.12424.. PMID: 36343938]
REFERENCE   6  (bases 1 to 14321)
  AUTHORS   Olczak,M., Maszczak-Seneczko,D., Sosicka,P., Jakimowicz,P. and
            Olczak,T.
  TITLE     UDP-Gal/UDP-GlcNAc chimeric transporter complements mutation defect
            in mammalian cells deficient in UDP-Gal transporter
  JOURNAL   Biochem Biophys Res Commun 434 (3), 473-478 (2013)
   PUBMED   23583405
  REMARK    GeneRIF: The data further supports the hypothesis that UGT and NGT
            cooperate in the UDP-Gal delivery for glycosyltransferases located
            in the Golgi apparatus.
REFERENCE   7  (bases 1 to 14321)
  AUTHORS   Maszczak-Seneczko,D., Sosicka,P., Majkowski,M., Olczak,T. and
            Olczak,M.
  TITLE     UDP-N-acetylglucosamine transporter and UDP-galactose transporter
            form heterologous complexes in the Golgi membrane
  JOURNAL   FEBS Lett 586 (23), 4082-4087 (2012)
   PUBMED   23089177
REFERENCE   8  (bases 1 to 14321)
  AUTHORS   Ghebranious,N., Burmester,J.K., Glurich,I., McPherson,E.,
            Ivacic,L., Kislow,J., Rasmussen,K., Kumar,V., Raggio,C.L.,
            Blank,R.D., Jacobsen,F.S., Faciszewski,T., Womack,J. and
            Giampietro,P.F.
  TITLE     Evaluation of SLC35A3 as a candidate gene for human vertebral
            malformations
  JOURNAL   Am J Med Genet A 140 (12), 1346-1348 (2006)
   PUBMED   16691598
  REMARK    GeneRIF: SLC35A3 is an unlikely candidate for the pathogenesis of
            vertebral malformations because no mutation was found in this
            cohort study.
REFERENCE   9  (bases 1 to 14321)
  AUTHORS   Thomsen,B., Horn,P., Panitz,F., Bendixen,E., Petersen,A.H.,
            Holm,L.E., Nielsen,V.H., Agerholm,J.S., Arnbjerg,J. and Bendixen,C.
  TITLE     A missense mutation in the bovine SLC35A3 gene, encoding a
            UDP-N-acetylglucosamine transporter, causes complex vertebral
            malformation
  JOURNAL   Genome Res 16 (1), 97-105 (2006)
   PUBMED   16344554
  REMARK    GeneRIF: A mutation in the SLC35A3 gene is associated with
            vertebral malformations in cattle. A missense mutation likely
            effects signal transduction which relies on glycosylation.
REFERENCE   10 (bases 1 to 14321)
  AUTHORS   Ishida,N., Yoshioka,S., Chiba,Y., Takeuchi,M. and Kawakita,M.
  TITLE     Molecular cloning and functional expression of the human Golgi
            UDP-N-acetylglucosamine transporter
  JOURNAL   J Biochem 126 (1), 68-77 (1999)
   PUBMED   10393322
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB021981.1, AC118553.3,
            CR749816.1 and AC093019.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_012243.2.
            
            Summary: This gene encodes a UDP-N-acetylglucosamine transporter
            found in the golgi apparatus membrane. In cattle, a missense
            mutation in this gene causes complex vertebral malformation.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Nov 2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.115194.1,
                                           SRR1660803.108665.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000533028.8/ ENSP00000433849.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2137              AB021981.1         13-2149
            2138-5599           AC118553.3         129353-132814
            5600-5666           CR749816.1         5149-5215
            5667-11473          AC118553.3         132882-138688
            11474-14321         AC093019.2         42890-45737
FEATURES             Location/Qualifiers
     source          1..14321
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p21.2"
     gene            1..14321
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
                     /note="solute carrier family 35 member A3"
                     /db_xref="GeneID:23443"
                     /db_xref="HGNC:HGNC:11023"
                     /db_xref="MIM:605632"
     exon            1..167
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
                     /inference="alignment:Splign:2.1.0"
     exon            168..372
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
                     /inference="alignment:Splign:2.1.0"
     CDS             186..1163
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
                     /note="isoform 1 is encoded by transcript variant 1;
                     solute carrier family 35 (UDP-N-acetylglucosamine
                     (UDP-GlcNAc) transporter), member 3; golgi UDP-GlcNAc
                     transporter; solute carrier family 35
                     (UDP-N-acetylglucosamine (UDP-GlcNAc) transporter), member
                     A3"
                     /codon_start=1
                     /product="UDP-N-acetylglucosamine transporter isoform 1"
                     /protein_id="NP_036375.1"
                     /db_xref="CCDS:CCDS762.1"
                     /db_xref="GeneID:23443"
                     /db_xref="HGNC:HGNC:11023"
                     /db_xref="MIM:605632"
                     /translation="MFANLKYVSLGILVFQTTSLVLTMRYSRTLKEEGPRYLSSTAVV
                     VAELLKIMACILLVYKDSKCSLRALNRVLHDEILNKPMETLKLAIPSGIYTLQNNLLY
                     VALSNLDAATYQVTYQLKILTTALFSVSMLSKKLGVYQWLSLVILMTGVAFVQWPSDS
                     QLDSKELSAGSQFVGLMAVLTACFSSGFAGVYFEKILKETKQSVWIRNIQLGFFGSIF
                     GLMGVYIYDGELVSKNGFFQGYNRLTWIVVVLQALGGLVIAAVIKYADNILKGFATSL
                     SIILSTLISYFWLQDFVPTSVFFLGAILVITATFLYGYDPKPAGNPTKA"
     exon            373..527
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
                     /inference="alignment:Splign:2.1.0"
     exon            528..650
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
                     /inference="alignment:Splign:2.1.0"
     exon            651..819
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
                     /inference="alignment:Splign:2.1.0"
     exon            820..938
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
                     /inference="alignment:Splign:2.1.0"
     exon            939..1072
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..14321
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2118..2123
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
                     /note="hexamer: ATTAAA"
     polyA_site      2138
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
                     /note="major polyA site"
     regulatory      5539..5544
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
                     /note="hexamer: TATAAA"
     polyA_site      5575
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
                     /note="major polyA site"
     regulatory      5647..5652
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
                     /note="hexamer: CATAAA"
     polyA_site      5666
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
     regulatory      14301..14306
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
                     /note="hexamer: AATAAA"
     polyA_site      14321
                     /gene="SLC35A3"
                     /gene_synonym="AMRS"
ORIGIN      
        1 ggaaacgcct gggctgcacc gagctggcgg tggctacggc gacgggagcc ggcggcgctg
       61 cgggtcagcg gtcgcgtagg acccagcgga ctcggcagcc tggggcgccc ggcggagctg
      121 aaccgcggcc cccggtggtg ggctcagccg gtcgagctgc gcgggaggca aatgaagata
      181 aaacaatgtt cgccaaccta aaatacgttt ccctgggaat tttggtcttt cagactacca
      241 gtttggttct aacaatgcgt tattccagaa ctttaaaaga agaaggacct cgttatctat
      301 cttctacagc agtggttgtt gctgaacttt tgaagataat ggcctgcatt ttattggtct
      361 acaaagacag caaatgtagt ctaagagcac tgaatcgagt actacatgat gaaattctta
      421 ataaacctat ggaaacactt aaacttgcta ttccatcagg gatctatact cttcagaata
      481 atttactgta tgtggcacta tcaaatctag atgcagctac ttatcaggtc acgtatcagt
      541 taaaaattct tacaacagca ttattttctg tgtctatgct tagtaaaaaa ttgggtgtat
      601 accagtggct gtccctagta attttgatga caggagttgc ttttgtacag tggccctcag
      661 attctcagct tgattctaag gaactttcag ctggttctca atttgtagga ctcatggcag
      721 ttctcacagc atgtttttca agtggctttg ctggggttta ctttgagaaa atcttaaaag
      781 aaacaaaaca atcagtgtgg ataagaaata ttcagcttgg tttctttgga agtatatttg
      841 gattaatggg tgtatacatt tatgatggag aactggtatc aaagaatgga ttttttcagg
      901 gatataaccg actgacctgg atagtagttg ttcttcaggc acttggaggc cttgtaatag
      961 ctgctgttat taagtatgca gataatattt taaaaggatt tgcaacctct ttatcgataa
     1021 tattatcaac attgatctcc tatttttggc ttcaagattt tgtgccaacc agtgtctttt
     1081 tccttggagc catccttgta ataacagcta cttttttgta tggttatgat cccaaacctg
     1141 caggaaatcc cactaaagca tagttgtata ctatctttaa ctggtttttc acgatggggc
     1201 actaggaatc tcgacattaa tcttgcacag aggacttcta cagagtctga gaagatatca
     1261 tcatgctgaa tctgatcata ctgtttttta aaagtttaag gataagacat gtgtatatgt
     1321 aacaaaacac attgcatcta gaaatcaaaa cttgaaagta tttccaggga ttaggattag
     1381 aaggaatatt agaggaaact tgaaatctga gtttaaaaag attttacctt tttgattgct
     1441 gcagaaatgt cctatgcact ctttgcaaga gcacacaaca aatgtcagat accaattttt
     1501 gcaaattaga tttaatctta ttaaatgttt ttatcttact ctttctgtac agatatatca
     1561 aatcacatga aatatttaaa gtttgaaaat tataattacc tataaagctg tgaaaaatag
     1621 aagtataatt tgaaaaaaca tttcacttat cagagatttt tatatttata caaaagatta
     1681 ctaaatgaag gattgctaaa tgtttttggt tcaattacat aaaaattaat attctgggtc
     1741 tgatctgtca gagaataaat atcaaatcta aatttaatgt agagatacat actatttctc
     1801 catatgaatt ttaagatatt ttagtgcttc aagactgctg aaagcaatcc agttgctcct
     1861 gtgctagatg gtagccagag aattttatag taatggaggt tagcccttaa tctcttcatt
     1921 gcatttcatt tctgtaaatc agattaagtc cttaatatta ttttaaatta aaatttgtgt
     1981 gtaattgcca ttaaattttc aaaatgtaat ttaaaaggat taaatactca tttaataatt
     2041 taaaataatt attgtataat atctacattt ggagaatttt gaactatcaa gcatatactg
     2101 tatacagtta gaaagttatt aaatgaacat tttactcatt gatctgtaaa aacttcttta
     2161 atctacaact gtttacaaaa acaacattca aacaaatagt acagatgtca gtgacagaac
     2221 aaaatgactt ttcttggaga cattccagat tgccatatta ctttatttta aacagcgcta
     2281 tgactttaaa tccaaggctg ctcggaagat ttttttaggt ctctcataag cctattcttc
     2341 cctgatcaca tgagtgggag aggtagccaa attttgaatt ccctttctgt gttcccacaa
     2401 gagctgctga gaaggcctgg cgcagtggct cacgcctgta atcctagcag tttgggaggc
     2461 tgaggcaggt ggatcacaag gtcaagagat tgagactatc ctgaccaaca tggtgaaacc
     2521 ccgtctctac caaaaataca aaaattagct gggtgtggtg gcacgcacct gtagtcccag
     2581 ctactcggga gactgaggca ggagaatcac ttgaacccgg gaggcagagg ttgcagtaag
     2641 tggagatcac gccactgcac tccaacctgg gcgacacagt gagagtctgt ctcaaaaaaa
     2701 agaaaaaaaa aaaagctact gagaaggagc caccattttg ctttaaaata gtgggttttt
     2761 ttccttttta atgaacactg gagctgagga tgcagattac atgagttatt taataagttg
     2821 attcaagaga gagagagtat attagaaatg gtaagatgat ttggtgggca aaaatgcttt
     2881 ctataatttg aatatgggtt taatttaatt attaactata gacagaatta cttactgagt
     2941 ataagaagta ttttactgtc tgggcacagt ggctcatgcc tgtaatccca gcactttggg
     3001 aggccgaggc gggtggatca caaggtcagg aattcgagac cagcctggcc agcatggtga
     3061 aatcccatct ctactaataa tacaaaaatt agctgggcat ggtggtgcgc gcctgtagtc
     3121 ccagctactc aggaggctga ggcaggagaa tttcttgaac acaccaggtg gaagttgcag
     3181 tgagccaaga tcgtgccact gcaccccagc ctgggtgaca gagcgagact ccgtctcaaa
     3241 aaaaaaaaaa agaaaacaca cacacacaca cacacaccca cagtatgaat gaaaaaaata
     3301 aaatacttct tttttttttt ttttgagaca gagtctcact ttgtcgccag gctggagtgc
     3361 tgtggcgcga tctcggctta ctgcaacctc ccactccctg gttcaaggga ttctcctgcc
     3421 tcagcctctg agtagctggg attgcaggcg tgagccactg cgcccggcct atactgtata
     3481 tatttttaaa gactgttcta atagatataa aaactgtaaa aaataagtat ttttatatag
     3541 ctctcatgga ttttattaaa cagaattggc tcaaaaatac tatgttacag actgttgggt
     3601 acccttgcct aacgtgaact ggcagtgtta ccttgctttt gcagtaatag tctacagatt
     3661 gcaggtctca tcaattccat ccaaagttta aaagcattta aaattaccaa atctttaaaa
     3721 tcactttggt ggtgattcca aattggtacc aagcaaactt tctggatgcc caacatgatt
     3781 ttcagtaacc accctttaga gtatttgttt actaagttca ccacattttg aacatggtag
     3841 ttttagactg caataatatt tagacttaca ttattactta ctgctaagta aaatctaaat
     3901 cctgcaaatg cacagaattc aagctgaaat ataatgattt atgtttagct cacattgaag
     3961 tattggttgg ttacttatgt attaatgcag tgtgcattca catttaatca ggtttagtct
     4021 gtttctattt taataatttt aaaaaattat acaagcaaat tagatattag acatgttagt
     4081 tacaatggta acacattttt aggtgtcgaa acacaatttt caaaattcct aatgaaagtt
     4141 ataaaaatgt aaacaagaat tgtaaaaatg gacaaagtag tcaaatatat tttcaaagca
     4201 caattttatt agacaggcat aatttacatt ttgcttttct agtgggtttg aaaatgttta
     4261 ttggagattg ggctatgtag tttataattt ttaattcata aaaaagtaat catacatgag
     4321 aaggtagacc tgtgccctag gatcatgtca catatacaga taatgccatt tccttgtgtg
     4381 tgtgatgtgt gttttgatga cctccacagg ccttactgta tcaagctttt ataatgatga
     4441 ctccttcatt atttaaattc ctatactttt tatttgttat cacgcaacta ctttgttcaa
     4501 tgtgaaaatg tgctaactca tgggagaaga gtgccaattg atagttcttt tagcaattaa
     4561 gaatatggta tttgggaaga aaagtttgaa atgcaacaaa tggatatttc aacacagtag
     4621 tattatatta tcagttcttt agtaagtgat tttagagatg ttgtaggcta cttttacggt
     4681 ggaatatata gtatagagat gcaaaactta aatgtttaca tcaatttata ttgaatgtca
     4741 cataatttca tggaaggaaa ggtagcttga tatttagatt ctaagatata atctgaaagg
     4801 aaactaatta tgttctctac acttactgta atactgatta ttcttacata tcaaattatt
     4861 gaactttaaa aatttcattg tatagtcatt aaactgagtt gggttttttc ttaaagggtt
     4921 tagcatcact catttgattt acacattcac attataatat ttaattatca tgggtgtatg
     4981 ctttacataa aaaaggttta taaaagttat ttatgctata ttgaaagtca tcttaagaat
     5041 ctccaggtta tttaaagtag ttataggagc agagaacaag cacctttatc aaaatctggt
     5101 cctatgtgcc ttgctttacc aaatacctga tttttctgga gggtgttcct gtaattcaca
     5161 actgtagaca catgggcaaa attaggattt ttaagaataa atacatttct atttttttgg
     5221 ttgtttcaac attagctctt caaattcatt aacaaaatta aaataggtat attacaaaag
     5281 cataaacatt tgtgaacagt acttaaataa attgtgatac tattgctcca tcattgaact
     5341 ttttgaaact ttaacaattg tataaaactg tcagtttgtt gtttcatttg taattacaaa
     5401 ataatttaaa aactttttaa aataatttgg atcctgactt tgtctatatc tgtatttcat
     5461 ttgtttagaa agattctttt gggtttgata atgtaatttg tatatttaaa ttttttatgg
     5521 acataattca aaggaatgta taaattggtc ttttgttaaa tggcttttta attgataaac
     5581 ttctcttgtc attttttggt atccagctat tacctattta atagatttat tgaaatagat
     5641 tattttcata aagaactcta tacaaatctt tttctatatt tccttatttt cctatttacc
     5701 tgtgtctatg acctaaccta tgaattagtc ttctctcttt atatatcaaa aatgaattac
     5761 tgatcttttt ctctggctct gtagtatctc tatcactgtc acatgtgatc tttcttcctt
     5821 ttctctagcc catattctag catgaaatac tgggttggcc aggtgcagtg gctcattcct
     5881 gtaatcccaa cactttagga agccaaggca gaagaatcgc ttgagcccat gaatttgagg
     5941 ccagcctggg caacatagcc ttgtttctac aaaaaatctt aaaaataaaa ttagccaata
     6001 tgtgggcatg cacctgtggg gccagctact caggaggctg aggcagaagg attgcttgag
     6061 cccagcaatt tgatgctgca gtaaaccatg atgacactac tggactctag cctgagtgac
     6121 agtgagactc tgtctcaaaa aacaaacaaa acaaaaactg aaacaacaaa aaaagactgg
     6181 gtttatttaa gctagttaga atttatcttt ctatatgtta ataacagcct aacagatttt
     6241 ttgttttaaa tatctctagg ctagcctcaa ggttaagtaa ttatagaagt ttggtatgta
     6301 ttttcttcat aatttgaata taattgcttc cattgtgact gtcaattgaa tgcatggaga
     6361 tcaattgtga taatatacag gattttagtc ctatctctac tgctgaagta accttacaca
     6421 aaatactttg taaaaaaatc actaaagtgc cagcattttt aaagtgtata tttttctttg
     6481 gcaacctctc atgaaaagca ctaactaaaa atatttaata atcttttttg tattacagtg
     6541 cttcttttgt tggaaatata tcacaatcct caagttccac tgctatgcaa aagtatctta
     6601 gaatctgaat cttatagata atactacctt tttttttttt tttttttttt ttttgagatg
     6661 gtgtctcgct ctgtcaccta ggctgcagtg gtgtgacctc actgcaacct ctgcctccca
     6721 ggttcaagca attctcctgc ctcagcctcc ctagtaactg ggattacagg ccatgccacc
     6781 acgcccaact aatttttgta tttttagtag agacagggtt tcaccatgtt ggccaggctg
     6841 gtctcaaact cctgacctca ggtgatccgc ctgcctcggc ctcccaaagt gctgggttta
     6901 caggcgtgag ccgctgcgcc cggccaatac tgcctttcaa aagagactga tagtgaccag
     6961 cattagtaat catactggtg gggttttttg gggtgttttt ctgttttgtt ttttgttttt
     7021 tgtttttgag acaaggtctt cctcccgttg cctaggctgg agtgcggtga cacaatcttg
     7081 gctcactgca gccttgatag agcaagctca agtgatcctc ccaggagcct cggcccccaa
     7141 gcagctggga atacaggtgc gcgccaccat gcccagctga tttttgtatt tttttgtaga
     7201 gatggggttt tgccatgttg cccggattgg tctcaaactc ctgagcttca gtgatctgcc
     7261 tgccttggcc tcccaaagtg ttgggattac aggcgtgagc gaccacaccc agcccatatt
     7321 ggtctttctt actgttctta aaaagagaat tcctttaagg caggaccgat tacatataca
     7381 ctctagaaaa gaaaatagca aggaagaaat aaattgcctt caattaccaa agatttgagc
     7441 ttctgctatg gctgagagtg ttttggtcat tgcaaattca ggggtttccc aagttcaccc
     7501 tcagttctgg ctagaaagag aaaacttact aaaagctatt atactcacag tcatatttat
     7561 tacagagaaa ggaaatacaa attaaaacca gccaaaggaa gtgacacata aaacagagtc
     7621 taggagtggt ccaaacttga ggctttcggt gtccttttct tgtagcgtca tggaaggtgt
     7681 tatctactcc tgaccacaat gtttgacagt acacacatag tattgccatt cagggaagct
     7741 cacctaagct ttggtgtcca gatttttatt gagagaggct ctattagttg gcatggttgg
     7801 ttgatttttt tgcccatgta gttgtcctct ctttccaaca tctgcccctc cctggagatc
     7861 tggttgacat caagacttca gggcctcacc ataggttatc tcgttagcat aaactgtcaa
     7921 gtgttgtcta aggaacccac aatgaataat aaagacattc ctatcagtga gaactcccaa
     7981 agacttacac cagaactttc tttggatggg ccaaatttct tactacacaa agaccattca
     8041 tctctataca cttccttctg aattgatgag gatgatacaa gcaacgacaa ttcttctttt
     8101 cagagacttt taattttttt tttttttttt tttttgagat ggagtctcgc tctgtcactc
     8161 aggctggagt tcagcggcac gatctctgct cactgcaacc tccgcctcct gggtttgagc
     8221 aattctccta cctcagcctc ccaagtagct gggattactg gtgctcacca ccacgcccaa
     8281 ctaatttttg tatttttggt agagacgggt tttcaccatg ttggccaggc tggtctcgaa
     8341 ctcctgacct caggtgatct gccggccttg gcctcccaaa gtgctgggat tacaggcatg
     8401 agccatcaca cccagcccag agacttttaa tttatacatt gtcattttta attttagaat
     8461 atctgtcaaa tgattctgaa cataacatga atctagtgtg gaaaaatgtt tataatcaga
     8521 tattgtgtta agaacatata tatatatata gagagagaga gagcatagta ttgtcattta
     8581 gttttcactt cttatgttta atgacaactt tataaatgct ggactatttt aaactacaaa
     8641 tttaaaacat ggtttataaa ccttttacct gtactgcata ttaataaaca attttgaact
     8701 aattttaaat taggcattcc tatcaccatt ataaggaaga gctaaaacct gataccagtt
     8761 gttattttgg ctcacagtag ggcttcacta gtgttaggat cacattttcc tcccttggac
     8821 ctgagaatca ggtgtttttc gtttaatgca tttattttca ttcattcatt taggatttct
     8881 aaaatgttag aggtaatttg tttaaaacaa ataacatggt gttcttttgg aaatatacag
     8941 tttatcttag atatacactt tcctcatttt aaaggtagaa ctcactgcag gtttaaaaga
     9001 ataatgaata ttgttacagt gtttagtgat aatcactgaa aattcacgtt aaaatatact
     9061 ttaagcttgt ccaacgcgcg gcctgcaggc tgcatgcagc ccaggatggc tttgaatgtg
     9121 gctcaacaca aatttgtaaa ctttcttaaa acagtatgag atttttttgc aattttttaa
     9181 agctcatcag ctattattag ttactgtatt ttatatgtgg cccaagacaa ttcttcttct
     9241 ttaagggtga cccagggaag ccaaaagatt ggacacccca gctttaaatg tagagtattt
     9301 aagaggttca gttaaatgta ctaaatcaga cttgtgtgat ttagacacaa aatctgtgtg
     9361 tgtgtgtgtt tggtctttta aaagatactg catgtacaaa catgatttct aataattgag
     9421 ggtttattaa gtttttccct ttaggctaaa gttttatttt attctgcttg gatagtaaat
     9481 acagcatttt aaaatgatga tgctaagtgc ttttttaata tatgcatgct gcttcaatag
     9541 gtcttttaaa tatagccaag gcagggtaat atttcctgag tgatgataga attatagaaa
     9601 atcatgaaag atgtgtgccc atgtttcatt ttataactgt tccagtcata ttgctatgaa
     9661 ttctagaatt ttaaaacctg ccacacttat tttggatgtt atatacacat aatgcctcaa
     9721 atttccatta tttcatatta tttttctcca tttccctcgt gtcaagtttc attttgtttt
     9781 caatgttttg tttttataat attgccattt taatggcttc aatcatgcta tgacagattg
     9841 tgccaattta gctttaaaaa actcatgggc tacctccatg aactcacaca tgcggcattg
     9901 ttaaagaacc ttttttaaaa ttaaattttc tccagctttt cattttgcag catacaaaaa
     9961 aattgaaaaa ttggtatacc acctaaatcc aaccattatt aacattttgc cacactagct
    10021 ttatctctgt tgggacactt tacccctaca tactccagca tgcgtgacct aaagatatgg
    10081 acatcttctt aattaacctc agtgccatta tgacacctaa gaaaatttaa attaattcag
    10141 gtcacttcat ctaagttcaa attcaaatac aattgcccct ctgtaaacgt gggggattgg
    10201 ttccaggacc cccacctcgt gtataccaaa atttgggcac actcaagtcc ttaaggggaa
    10261 accgcgtata agaaaagtca gccctcccta tgggtgggtt tcacatcctg caaataactg
    10321 tgttttccat ctgcatttgg ctgaaaaaaa atctgcatat aagtggaccc atgcagttca
    10381 aatctgttgt tcaagagtca actgtattcc cagttgtctc aaaaatgatt ttttatagct
    10441 tttttgtttt ggaacgaggg taaaatcaag gttaatgctt tgtatttgat tgtctccttt
    10501 ggtcttgaag tccccttcct gcaccaccac ctgcccctca atggcttttt tataccaaga
    10561 gactgaaaat gctacctcta atgacttttc tccccatgat agtactcttt gaaggaccaa
    10621 agttactttg ctcctattat gtggtttgat ttgttccctt tattcctttc atgtcctttg
    10681 aactggaagt caagtctata ggtttgatta ccttcaggtt aaatgtattg ggtaaaaata
    10741 tttcacagat tttatattgt atcatattaa aaggcacaga taatctcatt attaatgttg
    10801 gtaattattt ggttaagatg atgactaccc cattgtaaag gttattgtaa agttctccct
    10861 gtggtaaagg tcatttgttc acccttagta atctatggag taatgtttta gcaccaggag
    10921 aatattctgt ttcccaatgc ctttagcatc aattgatgat tcttgactga atcatttatt
    10981 acactggggt gcaaaatggt gataaatttt ttttagtgct ctaaatttct tctacattta
    11041 ttagtttata ttctttttta aaataaaaaa gtatatttcc ctcctgtttc tctaatatcc
    11101 ctctttaaaa aaaagaccat gtcttcatga atttaaaaaa aattactcaa tatattatag
    11161 ccaatcggtc acagttcttt ttgatgctca tatatattgt gagcctctag cccatgtaag
    11221 tcacagcaag ctagctcttg ggtttttttt gagatggagt ttcactctgt cacccaagct
    11281 ggagtgcagt ggcacgatct cggctcactg caacctccac cctccgggtt caaatgattc
    11341 tccttcctca gcctcctgag tagctgggac tataggtgcc tgccaccgcg cctggctaat
    11401 tttttgtatt tttagtagag atggggtttc accatcttgg ccaggctggt cttgaactcc
    11461 tgacctcgtg atccacccgc ctcggcctcc caaagtgctg ggattacagg catgagccac
    11521 cgcgcccggc cactcttgtg ttttttaaac atgaccacat tagtctttta gtccttattt
    11581 gctttaggca caagaaatct gaggcttacc ttatatttgt cttgttccaa atttggatta
    11641 tcagctaatt ctccaaggag ccctatacct tttaagggga gtgggattta tttaattttt
    11701 tctttggagt ccttctagcc agtgaatgga atttattata gaatccattg ttactgagct
    11761 gtcattgttt ctaggccatt ttagtggaca tagctaggaa atacaatttt aaaagatcat
    11821 gaattcaaat taatatttac aactaaaatt tagcaacatg gccaggtgca gtggctcatc
    11881 cctgtaattc cagcactttg ggagtctgag gtgggaggat cacttaaggc caggggttca
    11941 agaccagcct gggcaacaga gtgagatcct gtctctacaa aaaaaaaaaa aaagataaat
    12001 tttttcgaaa agttttatat gaaaagtgta ctctgaaaaa atctagctgt catacctatc
    12061 cctccattcc taaccatctc ttataagtaa ttattatcca tctcttataa gtaattatta
    12121 taagtatttt cttagttttc caatttatct ttcatgtgtt tctttttaaa aagcaaacaa
    12181 atatgcatat tgctgtactt actgtgcctc tttcacaaca tggggtcttt ataaaatttc
    12241 tttggatgtt tagaaaggtt tgtatgctct aatttatact gataacattt tatataatgc
    12301 atttagagtc cccaatttgc ttgtctaggc ctctactatt taatttgaca cctttaaatg
    12361 atgttctttg actcttatct aattgtctat gtgaccgtca gtgaatatat tatctgttca
    12421 tattttctgt agtcttattt ctttttgatt agaacatata atatttacac actattttcc
    12481 catcctaatc cccacctttg taataaaatt aattaaaatt ttaaaaaagt atatttaata
    12541 acctactgat acttacctga agtctctctg gtcaactttt ggctggatga agctcttctg
    12601 tagagtattc aaggagggct tgtaagtact gtacaatatt ctctgagttg tgacatgttt
    12661 aaaatttctt ctggagccta gatacttaaa gtacaccttg gccatgtaga aaatccttga
    12721 caaacatttc cttttcttga gtttgtctga atttgtgctt cactgttggg ttgcttgatg
    12781 ttatcatcaa gaagtgtggc accacttgat ttttttccct tttggaaatg acattttgtt
    12841 tgttgttttt tacctagaga cccagataat tttttctata ttattttatc tatgatcctt
    12901 tttatctcat ttttgttctc atagctgttt tattcctctt ctctgtattt cttactttac
    12961 ttttatttgt gtctctcagg taccctgtga tttagttttc atttttagat gattttatct
    13021 tttcagtata ttgcctatgt ttgttcaact ctcattttac atcttactat tgagtttaaa
    13081 aatttttatt tgctgtgttc ttttatatgt gcaattaatg atattcatgt tggagtgttg
    13141 tatattattt gcctctgctt catattgtct agagggtagt attttctatt gattgaaaag
    13201 ttttcatttt ctgtttttta taatagcctt gtatggattt ttggtggctt ttctattcat
    13261 gattaaatgt gtaggatctt cttcaatcag caataacagg tggctctata gaatggaggg
    13321 tagaagggat gtgggtgact tactcagttt ttagttaaag aggaccctct tctgttagca
    13381 tggtgaagtg cagtttcttt aataaattgt gcatggtggg ggtgggattt ggattctgtg
    13441 atacaatctt gtttctttag gaatctttta cttttggcca cttgcctttc tttccaagga
    13501 atcccactcc ctttcaaggt gcctcatgaa ctgttttcat gaactttcca aacattggtt
    13561 tctgcttgtt tctaagcctg attcttggcc ttctcattaa ttttcaaaac ttccaatatc
    13621 cttccaaata attccctttt gcttacgtta gcgagtacta gtttgttagc cagtgttagt
    13681 ttctgttgat cctaaccaaa aaaccctaac tgagatatca gtctcttagc gcaaagtttg
    13741 tgaatacggt catccctcca tatgaggagg ggagtgggaa ttggttgtgg gacttccgtg
    13801 gatatccaaa tcagtggatg ctaaagtccc ttacataaaa tggcatagta tttggttatc
    13861 atcctcccca tacatcatct ctagattatt tatagtactt aatacaatgt aatgctgtgt
    13921 agtcatactg tattttttac ttgtattttt gttgttttgt gggatttaaa aaatattttt
    13981 attctgagga tagttgaatc cacaggatac tgagggccag ctgtattcac aacccaaatc
    14041 acatacaaag cgacaagttc atacacaata ggcctattag aacaggactg ttctctcttg
    14101 tttatcattg cagcctttct agcacaaagc ctgggacatt ctggacattt agtatgtgtt
    14161 aaatttctct tactacatta tttccaacag tatttactgc aatctgcaat taccttcctt
    14221 ttgttttgta actgtgtccc ccactagaat gtaagctctg tgcagatagt gtctcattta
    14281 ttgatgtatc cctggcatct aataaaacac tgacaacaca a
//
