LOCUS       NM_014580               2145 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens solute carrier family 2 member 8 (SLC2A8), transcript
            variant 1, mRNA.
ACCESSION   NM_014580
VERSION     NM_014580.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Janzen,C., Lei,M.Y.Y., Jeong,I.S.D., Ganguly,A., Sullivan,P.,
            Paharkova,V., Capodanno,G., Nakamura,H., Perry,A., Shin,B.C.,
            Lee,K.W. and Devaskar,S.U.
  TITLE     Humanin (HN) and glucose transporter 8 (GLUT8) in pregnancies
            complicated by intrauterine growth restriction
  JOURNAL   PLoS One 13 (3), e0193583 (2018)
   PUBMED   29590129
  REMARK    GeneRIF: Humanin (HN) and glucose transporter 8 (GLUT8) in
            pregnancies complicated by intrauterine growth restriction
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Mashima,M., Chiba,Y., Murakami,R., Uemura,N., Matsumoto,K.,
            Kawauchi,M., Kanenishi,K., Hata,T. and Ueno,M.
  TITLE     Glucose transporter 8 immunoreactivity in astrocytic and microglial
            cells in subependymal areas of human brains
  JOURNAL   Neurosci Lett 636, 90-94 (2017)
   PUBMED   27818355
  REMARK    GeneRIF: glucose/fructose is transported into the cytoplasm of
            vimentin- or GFAP-positive astrocytic and CD68- or HLA-DR-positive
            microglial cells located around the lateral ventricle
REFERENCE   3  (bases 1 to 2145)
  AUTHORS   Mayer,A.L., Higgins,C.B., Heitmeier,M.R., Kraft,T.E., Qian,X.,
            Crowley,J.R., Hyrc,K.L., Beatty,W.L., Yarasheski,K.E., Hruz,P.W.
            and DeBosch,B.J.
  TITLE     SLC2A8 (GLUT8) is a mammalian trehalose transporter required for
            trehalose-induced autophagy
  JOURNAL   Sci Rep 6, 38586 (2016)
   PUBMED   27922102
  REMARK    GeneRIF: these data suggest that cytoplasmic trehalose access is
            carrier-mediated, and that GLUT8 is a mammalian trehalose
            transporter required for hepatocyte trehalose-induced autophagy and
            signal transduction.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2145)
  AUTHORS   Murakami,R., Chiba,Y., Tsuboi,K., Matsumoto,K., Kawauchi,M.,
            Fujihara,R., Mashima,M., Kanenishi,K., Yamamoto,T. and Ueno,M.
  TITLE     Immunoreactivity of glucose transporter 8 is localized in the
            epithelial cells of the choroid plexus and in ependymal cells
  JOURNAL   Histochem Cell Biol 146 (2), 231-236 (2016)
   PUBMED   27160096
  REMARK    GeneRIF: The findings support the hypothesis of the transport of
            intravascularGLUT8 through the epithelial cells of the choroid
            plexus and the ependymal cells.
REFERENCE   5  (bases 1 to 2145)
  AUTHORS   DeBosch,B.J., Chen,Z., Saben,J.L., Finck,B.N. and Moley,K.H.
  TITLE     Glucose transporter 8 (GLUT8) mediates fructose-induced de novo
            lipogenesis and macrosteatosis
  JOURNAL   J Biol Chem 289 (16), 10989-10998 (2014)
   PUBMED   24519932
  REMARK    GeneRIF: GLUT8 is thus essential for hepatocyte fructose transport
            and fructose-induced macrosteatosis.
REFERENCE   6  (bases 1 to 2145)
  AUTHORS   Schmidt,U., Briese,S., Leicht,K., Schurmann,A., Joost,H.G. and
            Al-Hasani,H.
  TITLE     Endocytosis of the glucose transporter GLUT8 is mediated by
            interaction of a dileucine motif with the beta2-adaptin subunit of
            the AP-2 adaptor complex
  JOURNAL   J Cell Sci 119 (Pt 11), 2321-2331 (2006)
   PUBMED   16723738
  REMARK    GeneRIF: The recruitment of GLUT8 to the endocytic machinery occurs
            via direct interaction of the dileucine motif with beta2-adaptin,
            and that endocytosis might be the main site at which GLUT8 is
            likely to be regulated.
REFERENCE   7  (bases 1 to 2145)
  AUTHORS   Richardson,S., Neama,G., Phillips,T., Bell,S., Carter,S.D.,
            Moley,K.H., Moley,J.F., Vannucci,S.J. and Mobasheri,A.
  TITLE     Molecular characterization and partial cDNA cloning of facilitative
            glucose transporters expressed in human articular chondrocytes;
            stimulation of 2-deoxyglucose uptake by IGF-I and elevated MMP-2
            secretion by glucose deprivation
  JOURNAL   Osteoarthritis Cartilage 11 (2), 92-101 (2003)
   PUBMED   12554125
REFERENCE   8  (bases 1 to 2145)
  AUTHORS   Schurmann,A., Axer,H., Scheepers,A., Doege,H. and Joost,H.G.
  TITLE     The glucose transport facilitator GLUT8 is predominantly associated
            with the acrosomal region of mature spermatozoa
  JOURNAL   Cell Tissue Res 307 (2), 237-242 (2002)
   PUBMED   11845330
  REMARK    GeneRIF: This carrier protein is expressed in human tissues of the
            testis in a gonadotropin-dependent manner.
REFERENCE   9  (bases 1 to 2145)
  AUTHORS   Doege,H., Schurmann,A., Bahrenberg,G., Brauers,A. and Joost,H.G.
  TITLE     GLUT8, a novel member of the sugar transport facilitator family
            with glucose transport activity
  JOURNAL   J Biol Chem 275 (21), 16275-16280 (2000)
   PUBMED   10821868
REFERENCE   10 (bases 1 to 2145)
  AUTHORS   Ibberson,M., Uldry,M. and Thorens,B.
  TITLE     GLUTX1, a novel mammalian glucose transporter expressed in the
            central nervous system and insulin-sensitive tissues
  JOURNAL   J Biol Chem 275 (7), 4607-4612 (2000)
   PUBMED   10671487
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY025817.1, BC019043.2,
            AL445222.9 and AF289587.1.
            
            On May 17, 2019 this sequence version replaced NM_014580.4.
            
            Summary: This gene belongs to the solute carrier 2A family, which
            includes intracellular glucose transporters. Based on sequence
            comparison, the glucose transporters are grouped into three classes
            and this gene is a member of class II. The encoded protein, like
            other members of the family, contains several conserved residues
            and motifs and 12 transmembrane domains with both amino and
            carboxyl ends being on the cytosolic side of the membrane.
            Alternatively spliced transcript variants have been described for
            this gene. [provided by RefSeq, Nov 2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.546540.1,
                                           SRR3476690.484112.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373371.8/ ENSP00000362469.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                HY025817.1         32-48
            18-2129             BC019043.2         1-2112
            2130-2130           AL445222.9         63677-63677
            2131-2145           AF289587.1         1923-1937
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q33.3"
     gene            1..2145
                     /gene="SLC2A8"
                     /gene_synonym="GLUT8; GLUTX1"
                     /note="solute carrier family 2 member 8"
                     /db_xref="GeneID:29988"
                     /db_xref="HGNC:HGNC:13812"
                     /db_xref="MIM:605245"
     exon            1..118
                     /gene="SLC2A8"
                     /gene_synonym="GLUT8; GLUTX1"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..1496
                     /gene="SLC2A8"
                     /gene_synonym="GLUT8; GLUTX1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     glucose transporter type 8; glucose transporter type X1;
                     solute carrier family 2 (facilitated glucose transporter),
                     member 8"
                     /codon_start=1
                     /product="solute carrier family 2, facilitated glucose
                     transporter member 8 isoform 1"
                     /protein_id="NP_055395.2"
                     /db_xref="CCDS:CCDS6870.1"
                     /db_xref="GeneID:29988"
                     /db_xref="HGNC:HGNC:13812"
                     /db_xref="MIM:605245"
                     /translation="MTPEDPEETQPLLGPPGGSAPRGRRVFLAAFAAALGPLSFGFAL
                     GYSSPAIPSLQRAAPPAPRLDDAAASWFGAVVTLGAAAGGVLGGWLVDRAGRKLSLLL
                     CSVPFVAGFAVITAAQDVWMLLGGRLLTGLACGVASLVAPVYISEIAYPAVRGLLGSC
                     VQLMVVVGILLAYLAGWVLEWRWLAVLGCVPPSLMLLLMCFMPETPRFLLTQHRRQEA
                     MAALRFLWGSEQGWEDPPIGAEQSFHLALLRQPGIYKPFIIGVSLMAFQQLSGVNAVM
                     FYAETIFEEAKFKDSSLASVVVGVIQVLFTAVAALIMDRAGRRLLLVLSGVVMVFSTS
                     AFGAYFKLTQGGPGNSSHVAISAPVSAQPVDASVGLAWLAVGSMCLFIAGFAVGWGPI
                     PWLLMSEIFPLHVKGVATGICVLTNWLMAFLVTKEFSSLMEVLRPYGAFWLASAFCIF
                     SVLFTLFCVPETKGKTLEQITAHFEGR"
     exon            119..281
                     /gene="SLC2A8"
                     /gene_synonym="GLUT8; GLUTX1"
                     /inference="alignment:Splign:2.1.0"
     exon            282..488
                     /gene="SLC2A8"
                     /gene_synonym="GLUT8; GLUTX1"
                     /inference="alignment:Splign:2.1.0"
     exon            489..588
                     /gene="SLC2A8"
                     /gene_synonym="GLUT8; GLUTX1"
                     /inference="alignment:Splign:2.1.0"
     exon            589..785
                     /gene="SLC2A8"
                     /gene_synonym="GLUT8; GLUTX1"
                     /inference="alignment:Splign:2.1.0"
     exon            786..929
                     /gene="SLC2A8"
                     /gene_synonym="GLUT8; GLUTX1"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1038
                     /gene="SLC2A8"
                     /gene_synonym="GLUT8; GLUTX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1039..1212
                     /gene="SLC2A8"
                     /gene_synonym="GLUT8; GLUTX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1358
                     /gene="SLC2A8"
                     /gene_synonym="GLUT8; GLUTX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..2145
                     /gene="SLC2A8"
                     /gene_synonym="GLUT8; GLUTX1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1892..1897
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC2A8"
                     /gene_synonym="GLUT8; GLUTX1"
                     /note="hexamer: AATAAA"
     polyA_site      1915
                     /gene="SLC2A8"
                     /gene_synonym="GLUT8; GLUTX1"
                     /note="major polyA site"
ORIGIN      
        1 gcactcgcag ggcccgtggc ggttcaggcg ccagagctgg ccgatcggcg ttggccgccg
       61 acatgacgcc cgaggaccca gaggaaaccc agccgcttct ggggcctcct ggcggcagcg
      121 cgccccgcgg ccgccgcgtc ttcctcgccg ccttcgccgc tgccctgggc ccactcagct
      181 tcggcttcgc gctcggctac agctccccgg ccatccctag cctgcagcgc gccgcgcccc
      241 cggccccgcg cctggacgac gccgccgcct cctggttcgg ggctgtcgtg accctgggtg
      301 ccgcggcggg gggagtgctg ggcggctggc tggtggaccg cgccgggcgc aagctgagcc
      361 tcttgctgtg ctccgtgccc ttcgtggccg gctttgccgt catcaccgcg gcccaggacg
      421 tgtggatgct gctggggggc cgcctcctca ccggcctggc ctgcggtgtt gcctccctag
      481 tggccccggt ctacatctcc gaaatcgcct acccagcagt ccgggggttg ctcggctcct
      541 gtgtgcagct aatggtcgtc gtcggcatcc tcctggccta cctggcaggc tgggtgctgg
      601 agtggcgctg gctggctgtg ctgggctgcg tgcccccctc cctcatgctg cttctcatgt
      661 gcttcatgcc cgagaccccg cgcttcctgc tgactcagca caggcgccag gaggccatgg
      721 ccgccctgcg gttcctgtgg ggctccgagc agggctggga agaccccccc atcggggctg
      781 agcagagctt tcacctggcc ctgctgcggc agcccggcat ctacaagccc ttcatcatcg
      841 gcgtctccct gatggccttc cagcagctgt cgggggtcaa cgccgtcatg ttctatgcag
      901 agaccatctt tgaagaggcc aagttcaagg acagcagcct ggcctcggtc gtcgtgggtg
      961 tcatccaggt gctgttcaca gctgtggcgg ctctcatcat ggacagagca gggcggaggc
     1021 tgctcctggt cttgtcaggt gtggtcatgg tgttcagcac gagtgccttc ggcgcctact
     1081 tcaagctgac ccagggtggc cctggcaact cctcgcacgt ggccatctcg gcgcctgtct
     1141 ctgcacagcc tgttgatgcc agcgtggggc tggcctggct ggccgtgggc agcatgtgcc
     1201 tcttcatcgc cggctttgcg gtgggctggg ggcccatccc ctggctcctc atgtcagaga
     1261 tcttccctct gcatgtcaag ggcgtggcga caggcatctg cgtcctcacc aactggctca
     1321 tggcctttct cgtgaccaag gagttcagca gcctcatgga ggtcctcagg ccctatggag
     1381 ccttctggct tgcctccgct ttctgcatct tcagtgtcct tttcactttg ttctgtgtcc
     1441 ctgaaactaa aggaaagact ctggaacaaa tcacagccca ttttgagggg cgatgacagc
     1501 cactcactag gggatggagc aagcctgtga ctccaagctg ggcccaagcc cagagcccct
     1561 gcctgcccca ggggagccag aatccagccc cttggagcct tggtctgcag ggtccctcct
     1621 tcctgtcatg ctccctccag cccatgaccc ggggctagga ggctcactgc ctcctgttcc
     1681 agctcctgct gctgctctga ggactcagga acaccttcga gctttgcaga cctgcggtca
     1741 gccctccatg cgcaagacta aagcagcgga agaggaggtg ggcctctagg atctttgtct
     1801 tctggctgga ggtgcttttg gaggttgggt gctgggcatt cagtcgctcc tctcacgcgg
     1861 ctgccttatc gggaaggaaa tttgtttgcc aaataaagac tgacacagaa aatcaggtca
     1921 gtgtctctgg gctttgtgca agctcagttt gaaaagggtt tattcccatc actgcccagg
     1981 acaccctgtg gctttacttg ctcatggtca gccaagctta cccttcacac tgagaagtca
     2041 tttctggcta cttccttggg ctcagttccc tgggtcatca gccatcaaat cttgttgagt
     2101 ttaaaaaata aacagcaaaa aaaaacaaaa caaaacaaaa aaaaa
//
