LOCUS       NM_153247               5714 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens solute carrier family 29 member 4 (SLC29A4),
            transcript variant 2, mRNA.
ACCESSION   NM_153247
VERSION     NM_153247.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Liu,Z., Jia,X., Wu,P., Wu,B., Pan,Y., Zhong,S., Xiao,L., Song,Y.,
            Hu,J. and Zhou,K.
  TITLE     PMAT variant rs3889348 is associated with metformin-induced
            gastrointestinal among Chinese Type 2 diabetes patients
  JOURNAL   Pharmacogenomics 24 (10), 551-560 (2023)
   PUBMED   37458617
  REMARK    GeneRIF: PMAT variant rs3889348 is associated with
            metformin-induced gastrointestinal among Chinese Type 2 diabetes
            patients.
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Moeez,S., Khalid,S., Shaeen,S., Khalid,M., Zia,A., Gul,A., Niazi,R.
            and Khalid,Z.
  TITLE     Clinically significant findings of high-risk mutations in human
            SLC29A4 gene associated with diabetes mellitus type 2 in Pakistani
            population
  JOURNAL   J Biomol Struct Dyn 40 (23), 12660-12673 (2022)
   PUBMED   34551672
  REMARK    GeneRIF: Clinically significant findings of high-risk mutations in
            human SLC29A4 gene associated with diabetes mellitus type 2 in
            Pakistani population.
REFERENCE   3  (bases 1 to 5714)
  AUTHORS   Sekhar,G.N., Fleckney,A.L., Boyanova,S.T., Rupawala,H., Lo,R.,
            Wang,H., Farag,D.B., Rahman,K.M., Broadstock,M., Reeves,S. and
            Thomas,S.A.
  TITLE     Region-specific blood-brain barrier transporter changes leads to
            increased sensitivity to amisulpride in Alzheimer's disease
  JOURNAL   Fluids Barriers CNS 16 (1), 38 (2019)
   PUBMED   31842924
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5714)
  AUTHORS   Tandio,D., Vilas,G. and Hammond,J.R.
  TITLE     Bidirectional transport of 2-chloroadenosine by equilibrative
            nucleoside transporter 4 (hENT4): Evidence for allosteric kinetics
            at acidic pH
  JOURNAL   Sci Rep 9 (1), 13555 (2019)
   PUBMED   31537831
  REMARK    GeneRIF: Bidirectional transport of 2-chloroadenosine by
            equilibrative nucleoside transporter 4 (hENT4): Evidence for
            allosteric kinetics at acidic pH.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5714)
  AUTHORS   Dawed,A.Y., Zhou,K., van Leeuwen,N., Mahajan,A., Robertson,N.,
            Koivula,R., Elders,P.J.M., Rauh,S.P., Jones,A.G., Holl,R.W.,
            Stingl,J.C., Franks,P.W., McCarthy,M.I., 't Hart,L.M. and
            Pearson,E.R.
  CONSRTM   IMI DIRECT Consortium
  TITLE     Variation in the Plasma Membrane Monoamine Transporter (PMAT)
            (Encoded by SLC29A4) and Organic Cation Transporter 1 (OCT1)
            (Encoded by SLC22A1) and Gastrointestinal Intolerance to Metformin
            in Type 2 Diabetes: An IMI DIRECT Study
  JOURNAL   Diabetes Care 42 (6), 1027-1033 (2019)
   PUBMED   30885951
  REMARK    GeneRIF: In an adjusted logistic regression model, the G allele at
            rs3889348 (SLC29A4) was associated with metformin gastrointestinal
            intolerance.
REFERENCE   6  (bases 1 to 5714)
  AUTHORS   Xia,L., Engel,K., Zhou,M. and Wang,J.
  TITLE     Membrane localization and pH-dependent transport of a newly cloned
            organic cation transporter (PMAT) in kidney cells
  JOURNAL   Am J Physiol Renal Physiol 292 (2), F682-F690 (2007)
   PUBMED   17018840
  REMARK    GeneRIF: our data suggest that PMAT is expressed on the apical
            membranes of renal epithelial cells and may use luminal proton
            gradient to drive organic cation reabsorption in the kidney.
REFERENCE   7  (bases 1 to 5714)
  AUTHORS   Barnes,K., Dobrzynski,H., Foppolo,S., Beal,P.R., Ismat,F.,
            Scullion,E.R., Sun,L., Tellez,J., Ritzel,M.W., Claycomb,W.C.,
            Cass,C.E., Young,J.D., Billeter-Clark,R., Boyett,M.R. and
            Baldwin,S.A.
  TITLE     Distribution and functional characterization of equilibrative
            nucleoside transporter-4, a novel cardiac adenosine transporter
            activated at acidic pH
  JOURNAL   Circ Res 99 (5), 510-519 (2006)
   PUBMED   16873718
  REMARK    GeneRIF: ENT4, in addition to playing roles in cardiac serotonin
            transport, contributes to the regulation of extracellular adenosine
            concentrations, in particular under the acidotic conditions
            associated with ischemia.
REFERENCE   8  (bases 1 to 5714)
  AUTHORS   Engel,K. and Wang,J.
  TITLE     Interaction of organic cations with a newly identified plasma
            membrane monoamine transporter
  JOURNAL   Mol Pharmacol 68 (5), 1397-1407 (2005)
   PUBMED   16099839
  REMARK    GeneRIF: PMAT can function as a polyspecific organic cation
            transporter, which may play a role in organic cation transport in
            vivo.
REFERENCE   9  (bases 1 to 5714)
  AUTHORS   Engel,K., Zhou,M. and Wang,J.
  TITLE     Identification and characterization of a novel monoamine
            transporter in the human brain
  JOURNAL   J Biol Chem 279 (48), 50042-50049 (2004)
   PUBMED   15448143
  REMARK    GeneRIF: may be a novel low affinity transporter for biogenic
            amines, might supplement the high affinity transporters in the
            brain
REFERENCE   10 (bases 1 to 5714)
  AUTHORS   Baldwin,S.A., Beal,P.R., Yao,S.Y., King,A.E., Cass,C.E. and
            Young,J.D.
  TITLE     The equilibrative nucleoside transporter family, SLC29
  JOURNAL   Pflugers Arch 447 (5), 735-743 (2004)
   PUBMED   12838422
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY485959.1, BC047592.1,
            BU740709.1, BM069878.1, H07897.1 and AC093376.5.
            
            On Aug 13, 2020 this sequence version replaced NM_153247.3.
            
            Summary: This gene encodes a member of the SLC29A/ENT transporter
            protein family. The encoded membrane protein catalyzes the reuptake
            of monoamines into presynaptic neurons, thus determining the
            intensity and duration of monoamine neural signaling. It has been
            shown to transport several compounds, including serotonin,
            dopamine, and the neurotoxin 1-methyl-4-phenylpyridinium.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK092242.1, AY485959.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142348
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000396872.8/ ENSP00000380081.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2099              AY485959.1         14-2112
            2100-2632           BC047592.1         2076-2608
            2633-2820           BU740709.1         60-247              c
            2821-2862           BM069878.1         297-338
            2863-2875           H07897.1           20-32               c
            2876-5714           AC093376.5         126410-129248
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p22.1"
     gene            1..5714
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /note="solute carrier family 29 member 4"
                     /db_xref="GeneID:222962"
                     /db_xref="HGNC:HGNC:23097"
                     /db_xref="MIM:609149"
     exon            1..140
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /inference="alignment:Splign:2.1.0"
     exon            141..317
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             149..1741
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /note="isoform 1 is encoded by transcript variant 2;
                     plasma membrane monoamine transporter; equilibrative
                     nucleoside transporter 4; solute carrier family 29
                     (nucleoside transporters), member 4; solute carrier family
                     29 (equilibrative nucleoside transporter), member 4"
                     /codon_start=1
                     /product="equilibrative nucleoside transporter 4 isoform
                     1"
                     /protein_id="NP_694979.2"
                     /db_xref="CCDS:CCDS5340.1"
                     /db_xref="GeneID:222962"
                     /db_xref="HGNC:HGNC:23097"
                     /db_xref="MIM:609149"
                     /translation="MGSVGSQRLEEPSVAGTPDPGVVMSFTFDSHQLEEAAEAAQGQG
                     LRARGVPAFTDTTLDEPVPDDRYHAIYFAMLLAGVGFLLPYNSFITDVDYLHHKYPGT
                     SIVFDMSLTYILVALAAVLLNNVLVERLTLHTRITAGYLLALGPLLFISICDVWLQLF
                     SRDQAYAINLAAVGTVAFGCTVQQSSFYGYTGMLPKRYTQGVMTGESTAGVMISLSRI
                     LTKLLLPDERASTLIFFLVSVALELLCFLLHLLVRRSRFVLFYTTRPRDSHRGRPGLG
                     RGYGYRVHHDVVAGDVHFEHPAPALAPNESPKDSPAHEVTGSGGAYMRFDVPRPRVQR
                     SWPTFRALLLHRYVVARVIWADMLSIAVTYFITLCLFPGLESEIRHCILGEWLPILIM
                     AVFNLSDFVGKILAALPVDWRGTHLLACSCLRVVFIPLFILCVYPSGMPALRHPAWPC
                     IFSLLMGISNGYFGSVPMILAAGKVSPKQRELAGNTMTVSYMSGLTLGSAVAYCTYSL
                     TRDAHGSCLHASTANGSILAGL"
     exon            318..449
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /inference="alignment:Splign:2.1.0"
     exon            450..563
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /inference="alignment:Splign:2.1.0"
     exon            564..692
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /inference="alignment:Splign:2.1.0"
     exon            693..767
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /inference="alignment:Splign:2.1.0"
     exon            768..1030
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1031..1169
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1357
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1598
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1599..5714
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2836..2841
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /note="hexamer: ATTAAA"
     polyA_site      2862
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /note="major polyA site"
     regulatory      3755..3760
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /note="hexamer: AATACA"
     polyA_site      3774
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
     polyA_site      5664
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
     regulatory      5693..5698
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
                     /note="hexamer: AATAAT"
     polyA_site      5714
                     /gene="SLC29A4"
                     /gene_synonym="ENT4; PMAT"
ORIGIN      
        1 tgtgctcgtc gccgcgctcg tggaccgggg ccgggcggac tcggggaccg gcggaggacg
       61 ccgggcgcgc ccggcccgag gctgggggag cggggcggcg tggcgggcgc gccgaggacc
      121 ccaggccggg ccgggccgag aggctgccat gggctccgtg gggagccagc gccttgagga
      181 gcccagcgtg gcaggcacac cagacccggg cgtagtgatg agcttcacct tcgacagtca
      241 ccagctggag gaggcggcgg aggcggctca gggccagggc cttagggcca ggggcgtccc
      301 agctttcacg gatactacat tggacgagcc agtgcccgat gaccgttatc acgccatcta
      361 ctttgcgatg ctgctggctg gcgtgggctt cctgctgcca tacaacagct tcatcacgga
      421 cgtggactac ctgcatcaca agtacccagg gacctccatc gtgtttgaca tgagcctcac
      481 ctacatcttg gtggcactgg cagctgtcct cctgaacaac gtcctggtgg agagactgac
      541 cctgcacacc aggatcaccg caggctacct cttagccttg ggccctctcc tttttatcag
      601 catctgcgac gtgtggctgc agctcttctc tcgggaccag gcctacgcca tcaacctggc
      661 cgctgtgggc accgtggcct tcggctgcac agtgcagcaa tccagcttct acgggtacac
      721 ggggatgctg cccaagcggt acacgcaggg ggtgatgacc ggggagagca cggcgggcgt
      781 gatgatctct ctgagccgca tcctcacgaa gctgctgctg cccgacgagc gcgccagcac
      841 gctcatcttc ttcctggtgt cggtggcgct ggagctgctg tgtttcctgc tgcacctgtt
      901 agtgcggcgc agccgcttcg tgctcttcta taccacacgg ccgcgtgaca gccaccgggg
      961 caggccaggc ctgggcaggg gctatggcta ccgcgtgcac cacgacgttg tcgccgggga
     1021 cgtccacttc gagcacccag ccccggccct ggcccccaac gagtccccaa aggacagccc
     1081 agcccacgag gtgaccggca gcggcggggc ctacatgcgc tttgatgtgc cgcggccaag
     1141 ggtccagcgc agctggccca ccttcagagc cctgttactg caccgctacg tggtggcgcg
     1201 ggtgatctgg gccgacatgc tctccatcgc cgtgacctac ttcatcacgc tgtgcctgtt
     1261 ccccggcctc gagtctgaga tccgccactg catcctgggc gagtggctgc ccatcctcat
     1321 catggctgtg ttcaacctgt cagacttcgt gggcaagatc ctggcagccc tgcccgtgga
     1381 ctggcggggc acccacctgc tggcctgctc ctgcctgcgt gtggtcttca tccccctctt
     1441 catcctgtgc gtctacccca gcggcatgcc cgccctccgt caccccgcct ggccctgcat
     1501 cttctcactg ctcatgggca tcagcaacgg ctacttcggc agcgtgccca tgatcctggc
     1561 ggcaggcaaa gtgagcccca agcagcggga gctggcaggg aacaccatga ccgtgtccta
     1621 catgtcaggg ctgacgctgg ggtccgccgt ggcctactgc acctacagcc tcacccgcga
     1681 cgctcacggc agctgcctgc acgcctccac cgccaatggt tccatcctcg caggcctctg
     1741 agccagcccc gcccactgcc agggacgccg agggcctgac caggggcccc gaggcctgag
     1801 ggcccctccc ctgtccccac ctcagtgcct gcggggccct gagcctcccc ctgtgccagc
     1861 agccccactc cctcagggtc cagccatgcc ccaccctgga ctgaagttct gcaaagtcct
     1921 ccgaggaccg gaacacgttt ctgcgacccg gggctctggc cagcactgtg ttctgcgttt
     1981 ggtctcatac ctgcgtctac cttccatctg tgtccagcgg ccccggctcc agcccagcca
     2041 gcactctgca gggtcacacg caccgtgtcc ccacccagga cagcagacac ccgccagagt
     2101 gtgcgcgccc agtgactgca ccccggccct catcacccac cggcactgat cggggcaccg
     2161 cctggcccag cctccaccag ggacccctcc tcatgaactc tggagccctg agaggagagg
     2221 ggcagccccc caccttgtca ccctcagggc ttccccttct gtcctcattc ttagagactg
     2281 cttctcccaa acataacgcg ttagccatga aggagtcgga gccctgggtc cgaatggacc
     2341 cgcctgcggt ctgcatcagc ctctgggaaa ccacagcagt gatgccagct gggcacgtca
     2401 ggacctcccc acacacccac acgatgccac aggtcagggg gctgtgcctg actagggagc
     2461 cctcccattg ccttcctggc ccgggataga agaggggagg taagtctggg ggctacgaag
     2521 ccgggccccc acaccctggc tgaagtcagc ttgacctagg tcttgaccct catccagcaa
     2581 gggactcgac agacccaagg gtccctggaa cgtagggagg ggctgggggt cagtccagcc
     2641 cgggcctccc agaacaccag gcccgtgtgg gtggcaccct gaggtcaggg gatcctaagg
     2701 gtgtccttcc agagacggtg tttccagggg gaggaccgcc cccgcttcca gatccccggc
     2761 cccggctgtg actgccctgt ttcacccctg ctgtgtccca tcccccgtct gtccactaac
     2821 tgtaccgcac cggccattaa aagatgaagg cagaccgctg caccgccgcc tgcaagcaga
     2881 gtttgtgcgt ggacgggggc tgggactgcc atctcgggat accggtggaa ctcgggctgc
     2941 cccgcataag ccggtcctca gtcttcccat ctgtgaggtg ggccgggtgg atcagggagc
     3001 aggagggtgt cctcacactg ggtaggcttg gtttgtgggc agcgctgggt agtggggtga
     3061 gagctgggag cacttagggt ttgtcgctca ccttgaccgc ccgccccccc caccccttcg
     3121 tgaggatcag ggaggatggc cacatgcagc ccatttgaag gggaaacaaa gtgggcgggg
     3181 cttggcccct gcccctcagg gactgcactg catcctagga gtcagagcct cctggcttcg
     3241 agcctcatcc cttttgctga tttgtgcaat ttggacaaaa tcctgcactg ttctgtgcct
     3301 catttctttc tttttttatt ttttttttga gactgagtct tgctcttgtc acccaggctg
     3361 gagtgcagtg gcacgatctt ggctcactgc aatctctgcc ttccaggttc aagcgattct
     3421 cctgcctcag cctctcaagt agctgggatt acaggtacgc accaccacac ctggctaatt
     3481 tgtcttgtgg agaccgggtt tcaccatgtt ggccatgctg gtctcgaact cctgatctca
     3541 agtgatccat ctgtctcatc ctcccaaagt gttgggatta caggtgtgag ccactgcacc
     3601 cgtcctgtgc ctcatttttg ttttttcttt ctttacaaga caggctctcg ctctgtcact
     3661 gaggctggag tgcagtggtg ccatcatagc tcactgcagc ctccacctcc tgggctccag
     3721 tgatcctccc acctcagcct cccaaatagc tgtgaataca ggcgcgtgcc accacgtctg
     3781 gccagattct tttattttgt gtagagatgg ggtcttgctg ttgcccagac tggtctcaaa
     3841 gtctgggcct caagtgttcc ccctgccttg gcctcccaaa gtgccaggat tacaggtatg
     3901 agtcactgcg cctggcctgt tctgtcctta gcttccccaa ggaatggggc tggtccaggt
     3961 tttgatgtgg gtcccgtaaa ggagggcacg agggccacct atgctctggc aggcatcctc
     4021 cctggggccc aggtgggcgg ccacttgggc caagactgcg ccaggtgaca ccagagtgtt
     4081 tcccagagcc catgccgggc ccttcccttg cccgaggccg gctggctgcc ctccatgggt
     4141 taccctcttt gcaaagcccc gaatgtcctc tgcaggccac ccacactaac caggtcagtc
     4201 actgggcagc cagaccccca cctatagctt ccctaggccc cagtgcagag atgccgtcca
     4261 cccccatcta ggtgagggct tggggccact cttagcatta gtatcatttt cttctagtat
     4321 cattttgtgg taggcatggc tggaaatgtg cagctttgct tttttttttt ttttttttgg
     4381 aggagtttcg cttttgttgc ccaggctgga gtgcaatggc tcgatttcag ctcactgcaa
     4441 cctctgcctg ccgggttcaa gcgattctcc tgcctcagcc tcccgagtag ctgggattac
     4501 aggcatgtgc caccatgccc ggctaatttt gtgcttttag tagagacgag gtttctccat
     4561 gttggtcagg ctggtcttga actcccgacc tcaggtgatc cacctgcctc agcctcccaa
     4621 agtgctggga ttacaggcat gagccaccat gcctggcact ggttttttga gatggaatct
     4681 cgttatgtca ccccagctgg agggcagtgg cacgatctca gctcactgta acctttgccg
     4741 tgggttcaag cgattctcct gcctcagcct cccaagtagc tgggattaca ggcacgctcc
     4801 accacacccg gctaattttt ttgtattttt agtagagatg gggtttcacc atattggcca
     4861 ggctggtctt gaactcctga cctcaagtga tccgcctccc ttggcctccc aaagtgctgg
     4921 gattacaggc gtgagccacc gtgcccatcc aacttaactt ttaatttttt ctcatgtaaa
     4981 tttgttcaat ttcttttttt tttttttttt ttttttttga gacaatctct gtcaccccca
     5041 ggccagagtg cagtggtgcg atctcaactc actgcaacct ccacccgccg ggtccctgtg
     5101 attttcccac ctcagcctcc cgagtagctg ggattacagg tgtgggccac cacacccggc
     5161 taatttttgt atttttagta gagacggggt ttcaccacat tgctcaggct ggtctgaaac
     5221 tcctggcctg aagtgatcct cccgcctcgg cctcctaaag tgctgggatt acaggcacgc
     5281 tctaccacac ctggctaatt tttttttgta tttttagtag agatggggtt tcaccatatt
     5341 ggccaggctg gtctcgaact cctgacttca agtgatccgc ctgccttggc ctcccaaagt
     5401 gctggcatta caggcgtgag ccactgtgcc cagccgatga gctcatttag atggctgcat
     5461 ggggtcaccg cccctgcacg cgtggcaccc aacttcttcc agtcgaatcc tgcttcttgg
     5521 tgccctgata tggcagaatg cccccagaca ccacccctac tccagcccct gaaataaacc
     5581 actccagaca attttatttt tccaattaaa tcttttcttt ttttttatga aaaaagatca
     5641 cacagaattt gccaacaaac aaaattccaa aagaaacata aaaaaaaaaa ccaataattc
     5701 ccccaaaaaa caaa
//
