LOCUS       NM_004212               4371 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens solute carrier family 28 member 2 (SLC28A2), mRNA.
ACCESSION   NM_004212
VERSION     NM_004212.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Hau,R.K., Klein,R.R., Wright,S.H. and Cherrington,N.J.
  TITLE     Localization of Xenobiotic Transporters Expressed at the Human
            Blood-Testis Barrier
  JOURNAL   Drug Metab Dispos 50 (6), 770-780 (2022)
   PUBMED   35307651
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Yamamura,T., Narumi,K., Ohata,T., Satoh,H., Mori,T., Furugen,A.,
            Kobayashi,M. and Iseki,K.
  TITLE     Characterization of deoxyribonucleoside transport mediated by
            concentrative nucleoside transporters
  JOURNAL   Biochem Biophys Res Commun 558, 120-125 (2021)
   PUBMED   33910126
  REMARK    GeneRIF: Characterization of deoxyribonucleoside transport mediated
            by concentrative nucleoside transporters.
REFERENCE   3  (bases 1 to 4371)
  AUTHORS   Zhou,Z., Li,Z., Wang,C., Li,X., Cheng,X., Li,C. and Shi,Y.
  TITLE     Common variants in the SLC28A2 gene are associated with serum uric
            acid level and hyperuricemia and gout in Han Chinese
  JOURNAL   Hereditas 156, 4 (2019)
   PUBMED   30679935
  REMARK    GeneRIF: significant association of intron variants in the SLC28A2
            gene with both serum uric acid level and hyperuricemia phenotype,
            and another exonic variant with gout in Han Chinese
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4371)
  AUTHORS   Sweeney,M.D., Zhao,Z., Montagne,A., Nelson,A.R. and Zlokovic,B.V.
  TITLE     Blood-Brain Barrier: From Physiology to Disease and Back
  JOURNAL   Physiol Rev 99 (1), 21-78 (2019)
   PUBMED   30280653
  REMARK    Review article
REFERENCE   5  (bases 1 to 4371)
  AUTHORS   Mulinta,R., Yao,S.Y.M., Ng,A.M.L., Cass,C.E. and Young,J.D.
  TITLE     Substituted cysteine accessibility method (SCAM) analysis of the
            transport domain of human concentrative nucleoside transporter 3
            (hCNT3) and other family members reveals features of structural and
            functional importance
  JOURNAL   J Biol Chem 292 (23), 9505-9522 (2017)
   PUBMED   28385889
  REMARK    GeneRIF: results that validate the newly developed structural
            homology model of CNT membrane architecture for human CNTs,
            revealed extended conformationally mobile regions within
            transport-domain TMs, identified pore-lining residues of functional
            importance, and provided evidence of an emerging novel
            elevator-type mechanism of transporter function.
REFERENCE   6  (bases 1 to 4371)
  AUTHORS   Li,J.Y., Boado,R.J. and Pardridge,W.M.
  TITLE     Differential kinetics of transport of 2',3'-dideoxyinosine and
            adenosine via concentrative Na+ nucleoside transporter CNT2 cloned
            from rat blood-brain barrier
  JOURNAL   J Pharmacol Exp Ther 299 (2), 735-740 (2001)
   PUBMED   11602688
REFERENCE   7  (bases 1 to 4371)
  AUTHORS   Pennycooke,M., Chaudary,N., Shuralyova,I., Zhang,Y. and Coe,I.R.
  TITLE     Differential expression of human nucleoside transporters in normal
            and tumor tissue
  JOURNAL   Biochem Biophys Res Commun 280 (3), 951-959 (2001)
   PUBMED   11162617
REFERENCE   8  (bases 1 to 4371)
  AUTHORS   Loewen,S.K., Ng,A.M., Yao,S.Y., Cass,C.E., Baldwin,S.A. and
            Young,J.D.
  TITLE     Identification of amino acid residues responsible for the
            pyrimidine and purine nucleoside specificities of human
            concentrative Na(+) nucleoside cotransporters hCNT1 and hCNT2
  JOURNAL   J Biol Chem 274 (35), 24475-24484 (1999)
   PUBMED   10455109
REFERENCE   9  (bases 1 to 4371)
  AUTHORS   Ritzel,M.W., Yao,S.Y., Ng,A.M., Mackey,J.R., Cass,C.E. and
            Young,J.D.
  TITLE     Molecular cloning, functional expression and chromosomal
            localization of a cDNA encoding a human Na+/nucleoside
            cotransporter (hCNT2) selective for purine nucleosides and uridine
  JOURNAL   Mol Membr Biol 15 (4), 203-211 (1998)
   PUBMED   10087507
REFERENCE   10 (bases 1 to 4371)
  AUTHORS   Wang,J., Su,S.F., Dresser,M.J., Schaner,M.E., Washington,C.B. and
            Giacomini,K.M.
  TITLE     Na(+)-dependent purine nucleoside transporter from human kidney:
            cloning and functional characterization
  JOURNAL   Am J Physiol 273 (6), F1058-F1065 (1997)
   PUBMED   9435697
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK222709.1, BC112047.1, AK291974.1 and AC051619.7.
            
            On Nov 22, 2018 this sequence version replaced NM_004212.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U84392.1, AK291974.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000347644.8/ ENSP00000315006.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-287               AK222709.1         5-291
            288-2128            BC112047.1         267-2107
            2129-2459           AK291974.1         2104-2434
            2460-4371           AC051619.7         147231-149142
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q21.1"
     gene            1..4371
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /note="solute carrier family 28 member 2"
                     /db_xref="GeneID:9153"
                     /db_xref="HGNC:HGNC:11002"
                     /db_xref="MIM:606208"
     exon            1..45
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     exon            46..142
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..2038
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /note="solute carrier family 28 (sodium-coupled nucleoside
                     transporter), member 2; SPNT; CNT 2; concentrative
                     nucleoside transporter 2; sodium-coupled nucleoside
                     transporter 2; sodium/purine nucleoside co-transporter;
                     solute carrier family 28 (concentrative nucleoside
                     transporter), member 2; Na(+)/nucleoside cotransporter 2"
                     /codon_start=1
                     /product="sodium/nucleoside cotransporter 2"
                     /protein_id="NP_004203.2"
                     /db_xref="CCDS:CCDS10121.1"
                     /db_xref="GeneID:9153"
                     /db_xref="HGNC:HGNC:11002"
                     /db_xref="MIM:606208"
                     /translation="MEKASGRQSIALSTVETGTVNPGLELMEKEVEPEGSKRTDAQGH
                     SLGDGLGPSTYQRRSRWPFSKARSFCKTHASLFKKILLGLLCLAYAAYLLAACILNFQ
                     RALALFVITCLVIFVLVHSFLKKLLGKKLTRCLKPFENSRLRLWTKWVFAGVSLVGLI
                     LWLALDTAQRPEQLIPFAGICMFILILFACSKHHSAVSWRTVFSGLGLQFVFGILVIR
                     TDLGYTVFQWLGEQVQIFLNYTVAGSSFVFGDTLVKDVFAFQALPIIIFFGCVVSILY
                     YLGLVQWVVQKVAWFLQITMGTTATETLAVAGNIFVGMTEAPLLIRPYLGDMTLSEIH
                     AVMTGGFATISGTVLGAFIAFGVDASSLISASVMAAPCALASSKLAYPEVEESKFKSE
                     EGVKLPRGKERNVLEAASNGAVDAIGLATNVAANLIAFLAVLAFINAALSWLGELVDI
                     QGLTFQVICSYLLRPMVFMMGVEWTDCPMVAEMVGIKFFINEFVAYQQLSQYKNKRLS
                     GMEEWIEGEKQWISVRAEIITTFSLCGFANLSSIGITLGGLTSIVPHRKSDLSKVVVR
                     ALFTGACVSLISACMAGILYVPRGAEADCVSFPNTSFTNRTYETYMCCRGLFQSTSLN
                     GTNPPSFSGPWEDKEFSAMALTNCCGFYNNTVCA"
     exon            143..231
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     exon            232..323
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     exon            324..507
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     exon            508..649
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     exon            650..763
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     exon            764..841
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     exon            842..922
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1003
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1129
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1260
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1429
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1627
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1709
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..1808
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1809..1920
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1921..4371
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4349..4354
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /note="hexamer: AATAAA"
     polyA_site      4371
                     /gene="SLC28A2"
                     /gene_synonym="CNT2; HCNT2; HsT17153; SPNT1"
                     /note="major polyA site"
ORIGIN      
        1 agtccttcac tgaggagcca gagggaatca attccacaag ctgggttgag gagaacagga
       61 gatggagaaa gcaagtggaa gacagtccat tgctctgtcc acagtggaga ctggcacagt
      121 gaacccgggg ctggagctca tggaaaaaga agtagagcct gagggaagca agaggactga
      181 cgcacaagga cacagcctgg gggatggact gggcccttcc acttaccaga ggaggagtcg
      241 gtggcctttc agcaaagcaa gaagtttctg caaaacacac gccagcttgt tcaagaagat
      301 cctgttgggc ctgttgtgtt tggcctatgc tgcctatctc ctggcagctt gcatcttgaa
      361 tttccagagg gcactggcct tgtttgtcat cacctgcttg gtgatctttg tcctggttca
      421 ctcgtttttg aaaaagctcc tgggcaaaaa attaacaaga tgtctgaagc cctttgaaaa
      481 ctcccgcctg aggctttgga cgaaatgggt gtttgcagga gtctccttgg ttggccttat
      541 actgtggttg gctttagaca cagcccaaag gccagagcag ctgatcccct ttgcaggaat
      601 ctgcatgttc atccttatcc tctttgcctg ctccaaacac cacagcgcag tgtcctggag
      661 gacagtgttt tcgggcctag gtcttcaatt tgtctttggg atcttggtca tcagaactga
      721 tcttggatat actgtatttc agtggctggg agagcaggtc cagattttcc tgaactacac
      781 tgtggccggc tccagttttg tctttgggga tacactggtc aaggatgtct ttgcttttca
      841 ggccttacca atcatcattt tctttggatg tgtggtgtcc attctctact acctgggcct
      901 tgtgcaatgg gtagttcaga aggtcgcctg gtttttacaa atcactatgg gcaccactgc
      961 tacagagacc ctggctgtgg caggaaacat ctttgtgggt atgacagagg cacctctgct
     1021 catccgtccc taccttgggg acatgacact ctctgaaatc catgcggtga tgactggagg
     1081 gtttgccacc atttctggca ctgtgctggg agccttcata gcctttgggg ttgatgcatc
     1141 atccctgatt tctgcctctg tgatggccgc cccttgtgct ctcgcctcat caaagctagc
     1201 gtatccggaa gtggaggagt ccaagttcaa gagtgaggag ggggtaaagc tgccccgtgg
     1261 gaaggagagg aatgtcctgg aagctgccag caacggagcc gtagatgcca taggccttgc
     1321 tactaatgta gcagccaacc tgattgcctt tttggctgtg ttggccttca tcaatgctgc
     1381 cctctcctgg ctgggggaat tggtggacat acaggggctc actttccagg tcatctgctc
     1441 ctatctccta aggcccatgg ttttcatgat gggtgtagag tggacagact gtccaatggt
     1501 ggctgagatg gtgggaatca agttcttcat aaatgagttt gtggcttatc agcaactgtc
     1561 tcaatacaag aacaaacgtc tctctggaat ggaggagtgg attgagggag agaaacagtg
     1621 gatttctgtg agagctgaaa tcattacaac attttcactc tgtggatttg ccaatcttag
     1681 ttccatagga atcacacttg gaggcttgac atcaatagta cctcaccgga agagtgactt
     1741 gtccaaggtt gtggtcaggg ccctcttcac aggggcctgt gtatccctta tcagtgcctg
     1801 tatggcagga atcctctatg tccccagggg agctgaagct gactgtgtct ccttcccaaa
     1861 cacaagtttc accaatagaa cctatgagac ctacatgtgc tgcagagggc tctttcagag
     1921 tacttctctg aatggcacca accctccttc tttttctggt ccctgggaag ataaggagtt
     1981 cagtgctatg gcccttacta actgctgtgg attctacaac aataccgtct gtgcctaagg
     2041 ctgcttgatc tatttctata acagttttga tcttaaaagc tttgtgattg caaaggtgtt
     2101 tatgtactca gggtgcccac aactcactca ccaagatgtt taacagtaag taacagtaaa
     2161 tgtaaaagat tcattttggg ccgggctcag tggctcatgc ctgtaatccc agcactttgg
     2221 gaggccgagg cgggcggatc acaaggtcag gagatcgaga ccatcctggc taacacagtg
     2281 aaaccccgtc tctactaaaa atacaaaaaa ttagccggga gtggtgtcgg gcgactgtag
     2341 tcccagctac tcgcgaggct gaggcaggag aatggcgtga atccgggagg cggagcttgc
     2401 agcgagccaa gatcgcgcca ctgcactcca gcctgggtga cagagcgaga ctctgtctca
     2461 aaaaaaaaaa aaaaaaaaaa gattcatttt ggttcgctgt atcccaataa cgaaaaatag
     2521 cttttgtttt tccattattt ggctgaacca aaaaatggca ttcctcatgg gccctgcctg
     2581 aactttcatt tgaaaaaata agtattactg tccaacaatg atagactgga ttaagaaaat
     2641 gtggcacata tacaccatgg aatactatgc agccataaaa aatgatgagc tcatgtcctt
     2701 tgcagggaca tggatgaaat tggaaatcat cattctcagt aaactatcgc aaggacaaaa
     2761 aaccaaacac cacatgttct cactcataga tgggaattga acaatgagaa cacatggaca
     2821 caggaagggg aacatcacac tctggggact gttgtggggt gaggggaagg gggagggata
     2881 gcattaggag atatacctaa tgctaaatga cgagttaatg ggtgcagcac actagcatgg
     2941 cacatgtata catatgtaac taacctgcat attgtgcaca tgtaccctaa aacttaaagt
     3001 ataataataa taataaaaga aaaaacttca taaaataaag tgaagcaccc accaaaaaat
     3061 aaaataaata aaataaataa ataaaagaaa aaataagtat tactatgtca aaaaagtctt
     3121 acaaatcatg cttctagttg ccatcttaaa aattatgtga tgcagtgtgc tataaatgtg
     3181 atttagtatg taatttgcct gggacggggg agaagctaca tttattggca ttgtggatga
     3241 gctggattta tattactgat gttacctttg aaaggtatat gaatatttaa tgataagatt
     3301 tttctggttg tcaaacattt gtaaatctgc aaacttctta acaacattac acagtaaaca
     3361 aaaaatactt cctgtatact aaggaagact tctaaggaat atccatttct taaaggccat
     3421 tactttgttg tagtattctg ctttagtgct agccacattt gctccaagtt tatggggaag
     3481 cctttaaaac agtaattttc agtgggaagg ggccaatttt gcttttcaga ggacatctga
     3541 cagtgtgtct ccagacatta ttggtgatta aagtgggagg atgctactga catctagtga
     3601 gtagaggcca atattgctac taaaattcct ataaccaaag gtcagctctc ccacaacgaa
     3661 gaattacctg gtcccaaacg tcaattgtgc tgagactcag aaactctgat tcaaaatatt
     3721 ttagatattt attatattta atattattta ttatattaat attatagcct taaggaaggg
     3781 tttcaaaagg atacaggagt tcctcaaaaa gttaaacaca gaattaccat agagccccac
     3841 aattccactc ctacatacac attcaaaaga atcaaaacta agttctcaaa caagtacatg
     3901 tacatgcatg ttcacagtag cactattcaa aatagccaaa aattggaaac agtctaaatg
     3961 tccatcaatg gataaattgt gttacataca cacaatggaa ttttattcac ccattaaaaa
     4021 aagaagtact gatttatgct atgtagaggc accccgaaaa catgctaaac gaaagaagct
     4081 agacacaaaa gtcttattat tgcatgattc catttagatg aaatattcag aatatataaa
     4141 tccatagaga cagaagggag atggtggttg ccagggcctg gggaaggagg gattagagag
     4201 tgactcctta acgagtttgg ggttttcctt tggggtgatg ggaatgtttt ggaacgagat
     4261 agaggtggtg gttgtgcaac attgtgaata ttatgtcact gaattgttca ctttaaaatg
     4321 gctgtttatg ttatgtgaat ttcacctcaa taaaaaaaat ttttagaagg a
//
