LOCUS       NM_004213               2770 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens solute carrier family 28 member 1 (SLC28A1),
            transcript variant 1, mRNA.
ACCESSION   NM_004213
VERSION     NM_004213.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Yamamura,T., Narumi,K., Ohata,T., Satoh,H., Mori,T., Furugen,A.,
            Kobayashi,M. and Iseki,K.
  TITLE     Characterization of deoxyribonucleoside transport mediated by
            concentrative nucleoside transporters
  JOURNAL   Biochem Biophys Res Commun 558, 120-125 (2021)
   PUBMED   33910126
  REMARK    GeneRIF: Characterization of deoxyribonucleoside transport mediated
            by concentrative nucleoside transporters.
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Altaweraqi,R.A., Yao,S.Y.M., Smith,K.M., Cass,C.E. and Young,J.D.
  TITLE     HPLC reveals novel features of nucleoside and nucleobase
            homeostasis, nucleoside metabolism and nucleoside transport
  JOURNAL   Biochim Biophys Acta Biomembr 1862 (7), 183247 (2020)
   PUBMED   32126230
REFERENCE   3  (bases 1 to 2770)
  AUTHORS   Jaramillo,A.C., Hubeek,I., Broekhuizen,R., Pastor-Anglada,M.,
            Kaspers,G.J.L., Jansen,G., Cloos,J. and Peters,G.J.
  TITLE     Expression of the nucleoside transporters hENT1 (SLC29) and hCNT1
            (SLC28) in pediatric acute myeloid leukemia
  JOURNAL   Nucleosides Nucleotides Nucleic Acids 39 (10-12), 1379-1388 (2020)
   PUBMED   32312148
  REMARK    GeneRIF: Expression of the nucleoside transporters hENT1 (SLC29)
            and hCNT1 (SLC28) in pediatric acute myeloid leukemia.
REFERENCE   4  (bases 1 to 2770)
  AUTHORS   Wevers,R.A., Christensen,M., Engelke,U.F.H., Geuer,S.,
            Coene,K.L.M., Kwast,J.T., Lund,A.M. and Vissers,L.E.L.M.
  TITLE     Functional disruption of pyrimidine nucleoside transporter CNT1
            results in a novel inborn error of metabolism with high excretion
            of uridine and cytidine
  JOURNAL   J Inherit Metab Dis 42 (3), 494-500 (2019)
   PUBMED   30847922
REFERENCE   5  (bases 1 to 2770)
  AUTHORS   Mulinta,R., Yao,S.Y.M., Ng,A.M.L., Cass,C.E. and Young,J.D.
  TITLE     Substituted cysteine accessibility method (SCAM) analysis of the
            transport domain of human concentrative nucleoside transporter 3
            (hCNT3) and other family members reveals features of structural and
            functional importance
  JOURNAL   J Biol Chem 292 (23), 9505-9522 (2017)
   PUBMED   28385889
  REMARK    GeneRIF: results that validate the newly developed structural
            homology model of CNT membrane architecture for human CNTs,
            revealed extended conformationally mobile regions within
            transport-domain TMs, identified pore-lining residues of functional
            importance, and provided evidence of an emerging novel
            elevator-type mechanism of transporter function.
REFERENCE   6  (bases 1 to 2770)
  AUTHORS   Mata,J.F., Garcia-Manteiga,J.M., Lostao,M.P., Fernandez-Veledo,S.,
            Guillen-Gomez,E., Larrayoz,I.M., Lloberas,J., Casado,F.J. and
            Pastor-Anglada,M.
  TITLE     Role of the human concentrative nucleoside transporter (hCNT1) in
            the cytotoxic action of 5[Prime]-deoxy-5-fluorouridine, an active
            intermediate metabolite of capecitabine, a novel oral anticancer
            drug
  JOURNAL   Mol Pharmacol 59 (6), 1542-1548 (2001)
   PUBMED   11353816
REFERENCE   7  (bases 1 to 2770)
  AUTHORS   Ritzel,M.W., Ng,A.M., Yao,S.Y., Graham,K., Loewen,S.K., Smith,K.M.,
            Hyde,R.J., Karpinski,E., Cass,C.E., Baldwin,S.A. and Young,J.D.
  TITLE     Recent molecular advances in studies of the concentrative
            Na+-dependent nucleoside transporter (CNT) family: identification
            and characterization of novel human and mouse proteins (hCNT3 and
            mCNT3) broadly selective for purine and pyrimidine nucleosides
            (system cib)
  JOURNAL   Mol Membr Biol 18 (1), 65-72 (2001)
   PUBMED   11396613
  REMARK    Review article
REFERENCE   8  (bases 1 to 2770)
  AUTHORS   Loewen,S.K., Ng,A.M., Yao,S.Y., Cass,C.E., Baldwin,S.A. and
            Young,J.D.
  TITLE     Identification of amino acid residues responsible for the
            pyrimidine and purine nucleoside specificities of human
            concentrative Na(+) nucleoside cotransporters hCNT1 and hCNT2
  JOURNAL   J Biol Chem 274 (35), 24475-24484 (1999)
   PUBMED   10455109
REFERENCE   9  (bases 1 to 2770)
  AUTHORS   Ritzel,M.W., Yao,S.Y., Ng,A.M., Mackey,J.R., Cass,C.E. and
            Young,J.D.
  TITLE     Molecular cloning, functional expression and chromosomal
            localization of a cDNA encoding a human Na+/nucleoside
            cotransporter (hCNT2) selective for purine nucleosides and uridine
  JOURNAL   Mol Membr Biol 15 (4), 203-211 (1998)
   PUBMED   10087507
REFERENCE   10 (bases 1 to 2770)
  AUTHORS   Ritzel,M.W., Yao,S.Y., Huang,M.Y., Elliott,J.F., Cass,C.E. and
            Young,J.D.
  TITLE     Molecular cloning and functional expression of cDNAs encoding a
            human Na+-nucleoside cotransporter (hCNT1)
  JOURNAL   Am J Physiol 272 (2 Pt 1), C707-C714 (1997)
   PUBMED   9124315
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK296074.1, U62968.1 and AC087468.11.
            
            On Nov 23, 2018 this sequence version replaced NM_004213.4.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1). Variants 1 and 4
            encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF309632.1, AK291997.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1970526, SAMEA2144835
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000394573.6/ ENSP00000378074.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-787               AK296074.1         1-787
            788-1589            U62968.1           581-1382
            1590-1590           AC087468.11        115259-115259       c
            1591-1782           U62968.1           1384-1575
            1783-1783           AC087468.11        114940-114940       c
            1784-2284           U62968.1           1577-2077
            2285-2770           AC087468.11        104640-105125       c
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q25.3"
     gene            1..2770
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /note="solute carrier family 28 member 1"
                     /db_xref="GeneID:9154"
                     /db_xref="HGNC:HGNC:11001"
                     /db_xref="MIM:606207"
     exon            1..90
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            91..206
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            207..318
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     CDS             223..2172
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sodium/nucleoside cotransporter 1; concentrative
                     nucleoside transporter 1; sodium-coupled nucleoside
                     transporter 1; Na(+)/nucleoside cotransporter 1; solute
                     carrier family 28 (sodium-coupled nucleoside transporter),
                     member 1; solute carrier family 28 (concentrative
                     nucleoside transporter), member 1"
                     /codon_start=1
                     /product="sodium/nucleoside cotransporter 1 isoform 1"
                     /protein_id="NP_004204.3"
                     /db_xref="CCDS:CCDS10334.1"
                     /db_xref="GeneID:9154"
                     /db_xref="HGNC:HGNC:11001"
                     /db_xref="MIM:606207"
                     /translation="MENDPSRRRESISLTPVAKGLENMGADFLESLEEGQLPRSDLSP
                     AEIRSSWSEAAPKPFSRWRNLQPALRARSFCREHMQLFRWIGTGLLCTGLSAFLLVAC
                     LLDFQRALALFVLTCVVLTFLGHRLLKRLLGPKLRRFLKPQGHPRLLLWFKRGLALAA
                     FLGLVLWLSLDTSQRPEQLVSFAGICVFVALLFACSKHHCAVSWRAVSWGLGLQFVLG
                     LLVIRTEPGFIAFEWLGEQIRIFLSYTKAGSSFVFGEALVKDVFAFQVLPIIVFFSCV
                     ISVLYHVGLMQWVILKIAWLMQVTMGTTATETLSVAGNIFVSQTEAPLLIRPYLADMT
                     LSEVHVVMTGGYATIAGSLLGAYISFGIDATSLIAASVMAAPCALALSKLVYPEVEES
                     KFRREEGVKLTYGDAQNLIEAASTGAAISVKVVANIAANLIAFLAVLDFINAALSWLG
                     DMVDIQGLSFQLICSYILRPVAFLMGVAWEDCPVVAELLGIKLFLNEFVAYQDLSKYK
                     QRRLAGAEEWVGDRKQWISVRAEVLTTFALCGFANFSSIGIMLGGLTSMVPQRKSDFS
                     QIVLRALFTGACVSLVNACMAGILYMPRGAEVDCMSLLNTTLSSSSFEIYQCCREAFQ
                     SVNPEFSPEALDNCCRFYNHTICAQ"
     exon            319..407
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            408..499
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            500..683
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            684..825
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            826..939
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1017
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1098
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1179
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1305
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1436
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            1437..1605
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            1606..1803
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            1804..1885
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..1984
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2096
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     exon            2097..2770
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2745..2750
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /note="hexamer: AATAAA"
     polyA_site      2770
                     /gene="SLC28A1"
                     /gene_synonym="CNT1; HCNT1; URCTU"
                     /note="major polyA site"
ORIGIN      
        1 caacgatgtg aaggttataa gctgcactgc atggttgctg ctggatgtgt tgtgttcctg
       61 gcttccctct ggatgctgac agaaacaagg gaatttctgc tgaagtgaca aggcaagcca
      121 gagccaaagc aggttggacc cagcttgtcc ccacagagac gtgtgcttcc ctctctctct
      181 gagagcgacc tgttaaccgc aaatacctgg aaggtctggg acatggagaa cgacccctcg
      241 agacgaagag agtccatctc tctcacacct gtggccaagg gtctggagaa catgggggct
      301 gatttcttgg aaagcctgga ggaaggccag ctccctagga gtgacttgag ccccgcagag
      361 atcaggagca gctggagcga ggcggcgccg aagcccttct ccagatggag gaacctgcag
      421 ccagccctga gagccagaag cttctgcagg gagcacatgc agctgtttcg atggatcggc
      481 acaggcctgc tctgcactgg gctctctgcc ttcctgctgg tggcctgcct cctggatttc
      541 cagagggccc tggctctgtt tgtcctcacc tgtgtggtcc tcaccttcct gggccaccgc
      601 ctgctgaaac ggcttctggg gccaaagctg aggaggtttc tcaagcctca gggccatccc
      661 cgcctgctgc tctggtttaa gaggggtcta gctcttgctg ctttcctggg cctggtcctg
      721 tggctgtctc tggacacctc ccagcggcct gagcaactgg tgtccttcgc aggaatctgc
      781 gtgttcgtcg ctctcctctt tgcctgctca aagcatcatt gcgcagtgtc ctggagggcc
      841 gtgtcttggg gacttggact gcagtttgta cttggactcc tcgtcatcag aacagaacca
      901 ggattcattg cgttcgagtg gctgggcgag cagatccgga tcttcctgag ctacacgaag
      961 gctggctcca gcttcgtgtt tggggaggcg ctggtcaagg atgtctttgc ctttcaggtt
     1021 ctgcccatca ttgtcttttt cagctgtgtc atatccgttc tctaccacgt gggcctcatg
     1081 cagtgggtga tcctgaagat tgcctggctg atgcaagtca ccatgggcac cacagccact
     1141 gagaccctga gtgtggctgg aaacatcttt gtgagccaga ccgaggctcc attactgatc
     1201 cggccctact tggcagacat gacactctct gaagtccacg ttgtcatgac cggaggttac
     1261 gccaccattg ctggcagcct gctgggtgcc tacatctcct ttgggatcga tgccacctcg
     1321 ttgattgcag cctctgtgat ggctgcccct tgtgccttgg ccctctccaa gctggtctac
     1381 ccggaggtgg aggagtccaa gtttaggagg gaggaaggag tgaaactgac ctatggagat
     1441 gctcagaacc tcatagaagc agccagcact ggggccgcca tctccgtgaa ggtggtcgcc
     1501 aacatcgctg ccaacctgat tgcgttcctg gctgtgctgg actttatcaa tgctgccctc
     1561 tcctggctgg gagacatggt ggacatccaa gggctcagct tccagctcat ctgctcctac
     1621 atcctgcggc ctgtagcctt cttgatgggt gtggcgtggg aggactgccc agtggtagct
     1681 gagctgctgg ggatcaagct gtttctgaac gagtttgtgg cctatcaaga cctctccaag
     1741 tacaagcaac gccgcctggc aggggccgag gagtgggtcg gcgacaggaa gcagtggatc
     1801 tccgtcagag ctgaagtcct cacgacgttt gccctctgtg gatttgccaa tttcagctcc
     1861 attgggatca tgctgggagg cttgacctcc atggtccccc aacggaagag cgacttctcc
     1921 cagatagtgc tccgggcgct cttcacggga gcctgtgtgt ccctggtgaa cgcctgtatg
     1981 gcagggatcc tctacatgcc caggggggct gaagttgact gcatgtccct cttgaacacg
     2041 accctcagca gcagtagctt tgagatttac cagtgctgcc gtgaggcctt ccagagcgtc
     2101 aatccagagt tcagcccaga ggccctggac aactgctgtc ggttttacaa ccacacgatc
     2161 tgtgcacagt gaggacagaa catgcttgtg cttctgcgct tctgagggct gttctccccc
     2221 gggaaccatc tgtccccacc ttccctttcc cagagccctc ttcagggaag ccacaggact
     2281 tagacccagc tcaatcccac aattgggaag ggttcatgga gtgagtgtgc agagagtgag
     2341 tgaggacata aggaaggaca tgtcccactc catccccctt cctgctcccc catttcctaa
     2401 ctcccccagt gtgaattctc agggtcactt ctgcctcctc ccgtttcccc tccacatcca
     2461 aacagcaccc tggtcctctc tatcccccct ctcctggggt ccctcacatg ccccttccct
     2521 tctgttgtgg gctgcacacc aaagcctcct cccctcccca cttcctaggc actaggatct
     2581 ctctgtggct tcccctgctg ggtggtgtca cctctttctc tgctttcaga gaaacccttc
     2641 ccgcctttcc tcagagtgct tcccaaactg aggtcccatg gcacactgtc ctgggaggcg
     2701 ttcagagggt tccatgatgg actaggtttg gaaccactgg gttaaataaa cttagagagg
     2761 gctgtttaga
//
