LOCUS       NM_030631               3893 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens solute carrier family 25 member 21 (SLC25A21),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_030631
VERSION     NM_030631.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Hu,S.S., Han,Y., Tan,T.Y., Chen,H., Gao,J.W., Wang,L., Yang,M.H.,
            Zhao,L., Wang,Y.Q., Ding,Y.Q. and Wang,S.
  TITLE     SLC25A21 downregulation promotes KRAS-mutant colorectal cancer
            progression by increasing glutamine anaplerosis
  JOURNAL   JCI Insight 8 (21), e167874 (2023)
   PUBMED   37937641
  REMARK    GeneRIF: SLC25A21 downregulation promotes KRAS-mutant colorectal
            cancer progression by increasing glutamine anaplerosis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 3893)
  AUTHORS   Boczonadi,V., King,M.S., Smith,A.C., Olahova,M., Bansagi,B.,
            Roos,A., Eyassu,F., Borchers,C., Ramesh,V., Lochmuller,H.,
            Polvikoski,T., Whittaker,R.G., Pyle,A., Griffin,H., Taylor,R.W.,
            Chinnery,P.F., Robinson,A.J., Kunji,E.R.S. and Horvath,R.
  TITLE     Mitochondrial oxodicarboxylate carrier deficiency is associated
            with mitochondrial DNA depletion and spinal muscular atrophy-like
            disease
  JOURNAL   Genet Med 20 (10), 1224-1235 (2018)
   PUBMED   29517768
  REMARK    GeneRIF: We identified a novel pathogenic variant in a patient by
            whole-exome sequencing. The pathogenicity of the mutation was
            studied by transport assays, computer modeling, followed by
            targeted metabolic testing and in vitro studies in human
            fibroblasts and neurons
            Erratum:[Genet Med. 2019 Sep;21(9):2163-2164. doi:
            10.1038/s41436-019-0506-1.. PMID: 31028354]
REFERENCE   4  (bases 1 to 3893)
  AUTHORS   Michailidou,K., Hall,P., Gonzalez-Neira,A., Ghoussaini,M.,
            Dennis,J., Milne,R.L., Schmidt,M.K., Chang-Claude,J., Bojesen,S.E.,
            Bolla,M.K., Wang,Q., Dicks,E., Lee,A., Turnbull,C., Rahman,N.,
            Fletcher,O., Peto,J., Gibson,L., Dos Santos Silva,I.,
            Nevanlinna,H., Muranen,T.A., Aittomaki,K., Blomqvist,C., Czene,K.,
            Irwanto,A., Liu,J., Waisfisz,Q., Meijers-Heijboer,H., Adank,M., van
            der Luijt,R.B., Hein,R., Dahmen,N., Beckman,L., Meindl,A.,
            Schmutzler,R.K., Muller-Myhsok,B., Lichtner,P., Hopper,J.L.,
            Southey,M.C., Makalic,E., Schmidt,D.F., Uitterlinden,A.G.,
            Hofman,A., Hunter,D.J., Chanock,S.J., Vincent,D., Bacot,F.,
            Tessier,D.C., Canisius,S., Wessels,L.F., Haiman,C.A., Shah,M.,
            Luben,R., Brown,J., Luccarini,C., Schoof,N., Humphreys,K., Li,J.,
            Nordestgaard,B.G., Nielsen,S.F., Flyger,H., Couch,F.J., Wang,X.,
            Vachon,C., Stevens,K.N., Lambrechts,D., Moisse,M., Paridaens,R.,
            Christiaens,M.R., Rudolph,A., Nickels,S., Flesch-Janys,D.,
            Johnson,N., Aitken,Z., Aaltonen,K., Heikkinen,T., Broeks,A.,
            Veer,L.J., van der Schoot,C.E., Guenel,P., Truong,T.,
            Laurent-Puig,P., Menegaux,F., Marme,F., Schneeweiss,A., Sohn,C.,
            Burwinkel,B., Zamora,M.P., Perez,J.I., Pita,G., Alonso,M.R.,
            Cox,A., Brock,I.W., Cross,S.S., Reed,M.W., Sawyer,E.J.,
            Tomlinson,I., Kerin,M.J., Miller,N., Henderson,B.E., Schumacher,F.,
            Le Marchand,L., Andrulis,I.L., Knight,J.A., Glendon,G.,
            Mulligan,A.M., Lindblom,A., Margolin,S., Hooning,M.J.,
            Hollestelle,A., van den Ouweland,A.M., Jager,A., Bui,Q.M.,
            Stone,J., Dite,G.S., Apicella,C., Tsimiklis,H., Giles,G.G.,
            Severi,G., Baglietto,L., Fasching,P.A., Haeberle,L., Ekici,A.B.,
            Beckmann,M.W., Brenner,H., Muller,H., Arndt,V., Stegmaier,C.,
            Swerdlow,A., Ashworth,A., Orr,N., Jones,M., Figueroa,J.,
            Lissowska,J., Brinton,L., Goldberg,M.S., Labreche,F., Dumont,M.,
            Winqvist,R., Pylkas,K., Jukkola-Vuorinen,A., Grip,M., Brauch,H.,
            Hamann,U., Bruning,T., Radice,P., Peterlongo,P., Manoukian,S.,
            Bonanni,B., Devilee,P., Tollenaar,R.A., Seynaeve,C., van
            Asperen,C.J., Jakubowska,A., Lubinski,J., Jaworska,K., Durda,K.,
            Mannermaa,A., Kataja,V., Kosma,V.M., Hartikainen,J.M.,
            Bogdanova,N.V., Antonenkova,N.N., Dork,T., Kristensen,V.N.,
            Anton-Culver,H., Slager,S., Toland,A.E., Edge,S., Fostira,F.,
            Kang,D., Yoo,K.Y., Noh,D.Y., Matsuo,K., Ito,H., Iwata,H., Sueta,A.,
            Wu,A.H., Tseng,C.C., Van Den Berg,D., Stram,D.O., Shu,X.O., Lu,W.,
            Gao,Y.T., Cai,H., Teo,S.H., Yip,C.H., Phuah,S.Y., Cornes,B.K.,
            Hartman,M., Miao,H., Lim,W.Y., Sng,J.H., Muir,K., Lophatananon,A.,
            Stewart-Brown,S., Siriwanarangsan,P., Shen,C.Y., Hsiung,C.N.,
            Wu,P.E., Ding,S.L., Sangrajrang,S., Gaborieau,V., Brennan,P.,
            McKay,J., Blot,W.J., Signorello,L.B., Cai,Q., Zheng,W.,
            Deming-Halverson,S., Shrubsole,M., Long,J., Simard,J.,
            Garcia-Closas,M., Pharoah,P.D., Chenevix-Trench,G., Dunning,A.M.,
            Benitez,J. and Easton,D.F.
  CONSRTM   Breast and Ovarian Cancer Susceptibility Collaboration; Hereditary
            Breast and Ovarian Cancer Research Group Netherlands (HEBON);
            kConFab Investigators; Australian Ovarian Cancer Study Group;
            GENICA (Gene Environment Interaction and Breast Cancer in Germany)
            Network
  TITLE     Large-scale genotyping identifies 41 new loci associated with
            breast cancer risk
  JOURNAL   Nat Genet 45 (4), 353-361 (2013)
   PUBMED   23535729
REFERENCE   5  (bases 1 to 3893)
  AUTHORS   Palmieri,F.
  TITLE     The mitochondrial transporter family SLC25: identification,
            properties and physiopathology
  JOURNAL   Mol Aspects Med 34 (2-3), 465-484 (2013)
   PUBMED   23266187
  REMARK    GeneRIF: Compares and contrasts all the known human SLC25A* genes
            and includes functional information.
            Review article
REFERENCE   6  (bases 1 to 3893)
  AUTHORS   Bailey,S.D., Xie,C., Do,R., Montpetit,A., Diaz,R., Mohan,V.,
            Keavney,B., Yusuf,S., Gerstein,H.C., Engert,J.C. and Anand,S.
  CONSRTM   DREAM investigators
  TITLE     Variation at the NFATC2 locus increases the risk of
            thiazolidinedione-induced edema in the Diabetes REduction
            Assessment with ramipril and rosiglitazone Medication (DREAM) study
  JOURNAL   Diabetes Care 33 (10), 2250-2253 (2010)
   PUBMED   20628086
  REMARK    GeneRIF: Observational study of gene-disease association,
            gene-environment interaction, and pharmacogenomic / toxicogenomic.
            (HuGE Navigator)
REFERENCE   7  (bases 1 to 3893)
  AUTHORS   Hendrickson,S.L., Lautenberger,J.A., Chinn,L.W., Malasky,M.,
            Sezgin,E., Kingsley,L.A., Goedert,J.J., Kirk,G.D., Gomperts,E.D.,
            Buchbinder,S.P., Troyer,J.L. and O'Brien,S.J.
  TITLE     Genetic variants in nuclear-encoded mitochondrial genes influence
            AIDS progression
  JOURNAL   PLoS One 5 (9), e12862 (2010)
   PUBMED   20877624
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 3893)
  AUTHORS   Talmud,P.J., Drenos,F., Shah,S., Shah,T., Palmen,J., Verzilli,C.,
            Gaunt,T.R., Pallas,J., Lovering,R., Li,K., Casas,J.P., Sofat,R.,
            Kumari,M., Rodriguez,S., Johnson,T., Newhouse,S.J., Dominiczak,A.,
            Samani,N.J., Caulfield,M., Sever,P., Stanton,A., Shields,D.C.,
            Padmanabhan,S., Melander,O., Hastie,C., Delles,C., Ebrahim,S.,
            Marmot,M.G., Smith,G.D., Lawlor,D.A., Munroe,P.B., Day,I.N.,
            Kivimaki,M., Whittaker,J., Humphries,S.E. and Hingorani,A.D.
  CONSRTM   ASCOT investigators; NORDIL investigators; BRIGHT Consortium
  TITLE     Gene-centric association signals for lipids and apolipoproteins
            identified via the HumanCVD BeadChip
  JOURNAL   Am J Hum Genet 85 (5), 628-642 (2009)
   PUBMED   19913121
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 3893)
  AUTHORS   Trynka,G., Zhernakova,A., Romanos,J., Franke,L., Hunt,K.A.,
            Turner,G., Bruinenberg,M., Heap,G.A., Platteel,M., Ryan,A.W., de
            Kovel,C., Holmes,G.K., Howdle,P.D., Walters,J.R., Sanders,D.S.,
            Mulder,C.J., Mearin,M.L., Verbeek,W.H., Trimble,V., Stevens,F.M.,
            Kelleher,D., Barisani,D., Bardella,M.T., McManus,R., van Heel,D.A.
            and Wijmenga,C.
  TITLE     Coeliac disease-associated risk variants in TNFAIP3 and REL
            implicate altered NF-kappaB signalling
  JOURNAL   Gut 58 (8), 1078-1083 (2009)
   PUBMED   19240061
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   10 (bases 1 to 3893)
  AUTHORS   Fiermonte,G., Dolce,V., Palmieri,L., Ventura,M., Runswick,M.J.,
            Palmieri,F. and Walker,J.E.
  TITLE     Identification of the human mitochondrial oxodicarboxylate carrier.
            Bacterial expression, reconstitution, functional characterization,
            tissue distribution, and chromosomal location
  JOURNAL   J Biol Chem 276 (11), 8225-8230 (2001)
   PUBMED   11083877
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AJ278148.1 and AL079303.3.
            
            On May 17, 2019 this sequence version replaced NM_030631.3.
            
            Summary: SLC25A21 is a homolog of the S. cerevisiae ODC proteins,
            mitochondrial carriers that transport C5-C7 oxodicarboxylates
            across inner mitochondrial membranes. One of the species
            transported by ODC is 2-oxoadipate, a common intermediate in the
            catabolism of lysine, tryptophan, and hydroxylysine in mammals.
            Within mitochondria, 2-oxoadipate is converted into
            acetyl-CoA.[supplied by OMIM, Apr 2004].
            
            Transcript Variant: This variant (1) is the longer transcript and
            encodes isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ278148.1, SRR5189667.301053.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2144120
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000331299.6/
                                                  ENSP00000329452.5
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1941              AJ278148.1         55-1995
            1942-3893           AL079303.3         115537-117488       c
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q13.3"
     gene            1..3893
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /note="solute carrier family 25 member 21"
                     /db_xref="GeneID:89874"
                     /db_xref="HGNC:HGNC:14411"
                     /db_xref="MIM:607571"
     exon            1..326
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             257..1156
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mitochondrial 2-oxodicarboxylate carrier; solute carrier
                     family 25 (mitochondrial oxodicarboxylate carrier), member
                     21; solute carrier family 25 (mitochondrial oxoadipate
                     carrier), member 21; mitochondrial 2-oxoadipate carrier"
                     /codon_start=1
                     /product="mitochondrial 2-oxodicarboxylate carrier isoform
                     1"
                     /protein_id="NP_085134.1"
                     /db_xref="CCDS:CCDS9663.1"
                     /db_xref="GeneID:89874"
                     /db_xref="HGNC:HGNC:14411"
                     /db_xref="MIM:607571"
                     /translation="MSAKPEVSLVREASRQIVAGGSAGLVEICLMHPLDVVKTRFQIQ
                     RCATDPNSYKSLVDSFRMIFQMEGLFGFYKGILPPILAETPKRAVKFFTFEQYKKLLG
                     YVSLSPALTFAIAGLGSGLTEAIVVNPFEVVKVGLQANRNTFAEQPSTVGYARQIIKK
                     EGWGLQGLNKGLTATLGRHGVFNMVYFGFYYNVKNMIPVNKDPILEFWRKFGIGLLSG
                     TIASVINIPFDVAKSRIQGPQPVPGEIKYRTCFKTMATVYQEEGILALYKGLLPKIMR
                     LGPGGAVMLLVYEYTYSWLQENW"
     exon            327..375
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /inference="alignment:Splign:2.1.0"
     exon            376..459
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /inference="alignment:Splign:2.1.0"
     exon            460..526
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /inference="alignment:Splign:2.1.0"
     exon            527..586
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /inference="alignment:Splign:2.1.0"
     exon            587..694
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /inference="alignment:Splign:2.1.0"
     exon            695..859
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /inference="alignment:Splign:2.1.0"
     exon            860..1041
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1094
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..3893
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1341..1346
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /note="hexamer: AATAAA"
     polyA_site      1364
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /note="major polyA site"
     regulatory      1436..1441
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /note="hexamer: AATAAA"
     polyA_site      1459
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
     regulatory      1921..1926
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /note="hexamer: AATAAA"
     polyA_site      1941
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /note="major polyA site"
     regulatory      2494..2499
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /note="hexamer: AATAAA"
     polyA_site      2518
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
     regulatory      2604..2609
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /note="hexamer: ATTAAA"
     polyA_site      2621
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
     regulatory      3471..3476
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
                     /note="hexamer: AATAAA"
     polyA_site      3495
                     /gene="SLC25A21"
                     /gene_synonym="MTDPS18; ODC; ODC1"
ORIGIN      
        1 cttctttcca gagacatttt cggagccgag ggcgcctctg cgagagcgct gcgaacaggt
       61 tccgacgcgc cggagatcgc gcgacgaatc tgcgcctcga accctcgctt tccagcagtc
      121 gccggattgc tgctccacag ggagccaacg cgcttgagga ccagcccagc cggggctctc
      181 tggatcagta ggctgtgcag gctgcgagct cgttgacgca tctcagccca ctcccttgtc
      241 ctcccgcctg gcgaagatgt ccgccaagcc tgaagtcagc ttagtgcgcg aggcttctcg
      301 gcagatcgtg gccggtggtt ctgcaggtct tgtagaaatt tgcctgatgc accccctaga
      361 tgtggtgaaa accaggtttc agattcagag atgtgcaacc gatccaaaca gttataaaag
      421 cttggtagac agctttcgaa tgattttcca aatggaaggg ttatttggtt tttacaaggg
      481 aattctgcca cctatcttgg ctgaaacccc aaaaagagca gtgaagtttt tcacctttga
      541 gcagtacaag aaattgctgg gatatgtgtc actgtcacca gcattgacat tcgccattgc
      601 tggattggga tctggactaa cagaagccat tgtagttaac ccttttgagg tagtaaaagt
      661 tggcttgcaa gcaaatcgga acacatttgc agagcaacca tccactgtgg gttatgcaag
      721 acaaatcatt aagaaggaag gctggggact ccagggcctc aacaaaggat taactgcaac
      781 tttgggacga catggagttt tcaacatggt ttattttggc ttctactaca atgtcaaaaa
      841 catgattcct gtcaataagg atccaatctt ggagttttgg agaaaatttg ggattggtct
      901 tctctcgggg acaatagcct cagtcattaa catccctttt gatgttgcca aaagtaggat
      961 tcaagggcct caaccagttc ctggagagat caagtacaga acctgtttta aaacaatggc
     1021 aacagtctat caggaagaag ggattttagc tttgtacaaa ggcctgcttc ccaagattat
     1081 gagacttgga ccaggtggtg cagtgatgct gctggtttat gaatacacct attcatggct
     1141 tcaagagaac tggtgattgc ctatgaagtg tttttccccc ttgagataat ggatatttgc
     1201 tatgcagcac catgaagaag agagactatc gatcggccag gtgtataatt atgaaggaga
     1261 aaactgttca agaacaaaat ctattttaat actttaaaaa atgtctatag gcaacttgag
     1321 attatagttt tggctgtata aataaaactg tgagaagaaa aaaataatat gaaacaatga
     1381 ggtatttaaa gtgaatatag aaaaatagca tagaaatcat atatttattt cataaaataa
     1441 atagaaactt tgtctcctgt gttcctcttc aatgcttcac aatgaaaatt cattgtattg
     1501 atcagcattt ttcctaagag gtctagaaat gcttaaacta ttgaaaataa atcaaattat
     1561 tcaaagactg gattaaaaaa aagttaagcc aaaatttaag cagtttttat aatgaattaa
     1621 attattccat aaataatatt taaagagcac aagaaaaatt ttttaaagtg cactaaaatg
     1681 accttttaag aatgtttaat tttatgaata ctatgttaaa atgtaaatct gttggttttt
     1741 tttagtaact atgcacatat cagctctatt ttcctacatc tactttatat ctcatattgc
     1801 tgtaagataa taaataggta ttttaaaaca ttgtttaaaa cagaatgtta aaacactgtt
     1861 gtttaagcat gtttaaatca aggtattcca aatctcttct aacacaagta ggaaacatga
     1921 aataaaattt actttcctcc acatagttct ctttgaagat aagttttgtt gatgagatat
     1981 tgtcatgtta aatcacaaat tggaaacacc tttggaccca tttgtcagcc atcagtattt
     2041 gcatccaaat acctttattc agcatgccta cctgtgccat ttttaggaag aatgtataga
     2101 acagaactgc attttcaggt tattagtttt aaacgacaac ttcaggtggg aagtcagctg
     2161 tctgcagtat taaaatatgt atgatgaagg ggaaatgttc tgaaaaagaa atacacttag
     2221 catttctaag gattactata ctaacatgta tcaacaaaaa tatctccttg gtcttgatgt
     2281 aaccaaactt gattttatat ctgcatccag agatgatgct gattcagtaa agtcaacaag
     2341 aataactgaa agtccatatg acacctgtcc taagttacta ccgtgggcta tttctactac
     2401 ggggctattt cactaaagca ttttgaaaat aaaaagtaaa agataacatg gtaattatta
     2461 ctttttatta atttagagca tttgaagtat aaaaataaaa ggcttttgac atactgtata
     2521 tacatacata gccttctgtt gtacatcctt tccaacgtgt tttttaaaat ttatatttca
     2581 gtccaatatt caataaaagg gtcattaaaa acaaaacaaa attgtgaaaa aaaagaaata
     2641 agaatgtgtc tctgttgcac aactgcattc tatccttgca ggtaatattc ttacatccaa
     2701 tgagagcgct gcctgcatag aggtcatgaa attgaacctt taacctctcc atgtggatca
     2761 gatagaaaag gatttctgaa gagtgcattt gccagtttaa aagcaacact tttaccatct
     2821 atgagataga ggaaatatgt caacattaac caataataat ctttaaaagt taacccttta
     2881 atccatccta ttttccttct cgtattttaa aaagtggctt taaatcataa ttttctttga
     2941 atagcaaatt gtatctgata tcttttaaaa attactccat gtgaattcac tgttaagacc
     3001 tagagggaga aaaggaaact tcaataccat taatattaga ttttttaaaa tacaagaacc
     3061 agaaaaaaat tataaatagc acaataacaa gagatttctt aatttagata aagaaaataa
     3121 cattatttgc atttccacac aagcaagtaa tattttttaa agccccctaa cagccaccta
     3181 aaaacatttg ataaagaata gttctgtacc ttacatcagt ttttcagatg acaggagagt
     3241 ccctgtatgg tatggtttta aagaagtctc caccaaaata ttggattgga tctttgttcc
     3301 attcagttta tagtctattt tctcaccaga ctgggaactc cagatgtcaa tggaagtttt
     3361 acaagatcaa aggaagacta tggccctgag agttcatctg ctccttctgc ttattcttct
     3421 cctgaagtta taatcctcta tttgtagcac tgaatttcgg ttgctttaga aataaatgca
     3481 gttcaaagct ctctataaga tatctgctgt ctgaaaaaag cagtcactct aacataattt
     3541 gtgtgtcaga aatctgctta ctgtttacat cctagtgatt tgaaaaccta actaatctag
     3601 ccatctgagt tttaaacgaa gccacgaaac cgatgtggtg gaaccgcatt gccatgtaga
     3661 ctgtgcacag cacagaccgg aagaaattaa aatcacctag ggaatccatc ttcaacatcc
     3721 atatccagtg aggtgctctt ttagttattg cacagaatta gtaccaaaaa accctcaata
     3781 tattaatttt ccaacagctg aattgtacca atgtcctaaa aacatatttt taagcctata
     3841 ttgccaaaca ggaagaactt cctaaacaga agggattaaa aactgtagaa ttt
//
