LOCUS       NM_153646              10388 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens solute carrier family 24 member 4 (SLC24A4),
            transcript variant 1, mRNA.
ACCESSION   NM_153646
VERSION     NM_153646.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Thibodeau,S., Yang,W., Sharma,S. and Lytton,J.
  TITLE     Calmodulin binds and modulates K+-dependent Na+/Ca2+-exchanger
            isoform 4, NCKX4
  JOURNAL   J Biol Chem 296, 100092 (2021)
   PUBMED   33199372
  REMARK    GeneRIF: Calmodulin binds and modulates K(+)-dependent
            Na(+)/Ca(2+)-exchanger isoform 4, NCKX4.
REFERENCE   2  (bases 1 to 10388)
  AUTHORS   Meyer,O.S., Lunn,M.M.B., Garcia,S.L., Kjaerbye,A.B., Morling,N.,
            Borsting,C. and Andersen,J.D.
  TITLE     Association between brown eye colour in rs12913832:GG individuals
            and SNPs in TYR, TYRP1, and SLC24A4
  JOURNAL   PLoS One 15 (9), e0239131 (2020)
   PUBMED   32915910
  REMARK    GeneRIF: Association between brown eye colour in rs12913832:GG
            individuals and SNPs in TYR, TYRP1, and SLC24A4.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10388)
  AUTHORS   Khan,S.A., Khan,M.A., Muhammad,N., Bashir,H., Khan,N., Muhammad,N.,
            Yilmaz,R., Khan,S. and Wasif,N.
  TITLE     A novel nonsense variant in SLC24A4 causing a rare form of
            amelogenesis imperfecta in a Pakistani family
  JOURNAL   BMC Med Genet 21 (1), 97 (2020)
   PUBMED   32380970
  REMARK    GeneRIF: This nonsense sequence variant c.1192C > T (p.Gln398*) is
            the sixth disease-causing variant in SLC24A4, which extends its
            mutation spectrum and confirms the role of this gene in the
            morphogenesis of human tooth enamel. The identified variant
            highlights the critical role of SLC24A4 in causing a rare
            Amelogenesis imperfecta (AI) type in humans.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10388)
  AUTHORS   Bronckers,A.L., Jalali,R. and Lytton,J.
  TITLE     Reduced Protein Expression of the Na+/Ca2++K+-Exchanger (SLC24A4)
            in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic
            Mice
  JOURNAL   Calcif Tissue Int 100 (1), 80-86 (2017)
   PUBMED   27752731
REFERENCE   5  (bases 1 to 10388)
  AUTHORS   Lu,H., Zhu,X.C., Wang,H.F., Cao,L., Tan,M.S., Tan,C.C., Jiang,T.,
            Yu,J.T. and Tan,L.
  TITLE     Lack of Association Between SLC24A4 Polymorphism and Late-onset
            Alzheimer's Disease in Han Chinese
  JOURNAL   Curr Neurovasc Res 13 (3), 239-243 (2016)
   PUBMED   27215332
  REMARK    GeneRIF: no significant relation was noted between SLC24A4
            rs10498633 and late-onset Alzheimer;s disease risk in neither
            apolipoprotein E (APOE) epsilon4 carriers nor non-carriers after
            adjusting for age and gender
REFERENCE   6  (bases 1 to 10388)
  AUTHORS   Duffy,D.L., Zhao,Z.Z., Sturm,R.A., Hayward,N.K., Martin,N.G. and
            Montgomery,G.W.
  TITLE     Multiple pigmentation gene polymorphisms account for a substantial
            proportion of risk of cutaneous malignant melanoma
  JOURNAL   J Invest Dermatol 130 (2), 520-528 (2010)
   PUBMED   19710684
  REMARK    GeneRIF: Observational study of gene-disease association and
            gene-gene interaction. (HuGE Navigator)
REFERENCE   7  (bases 1 to 10388)
  AUTHORS   Adeyemo,A., Gerry,N., Chen,G., Herbert,A., Doumatey,A., Huang,H.,
            Zhou,J., Lashley,K., Chen,Y., Christman,M. and Rotimi,C.
  TITLE     A genome-wide association study of hypertension and blood pressure
            in African Americans
  JOURNAL   PLoS Genet 5 (7), e1000564 (2009)
   PUBMED   19609347
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
REFERENCE   8  (bases 1 to 10388)
  AUTHORS   Han,J., Kraft,P., Nan,H., Guo,Q., Chen,C., Qureshi,A.,
            Hankinson,S.E., Hu,F.B., Duffy,D.L., Zhao,Z.Z., Martin,N.G.,
            Montgomery,G.W., Hayward,N.K., Thomas,G., Hoover,R.N., Chanock,S.
            and Hunter,D.J.
  TITLE     A genome-wide association study identifies novel alleles associated
            with hair color and skin pigmentation
  JOURNAL   PLoS Genet 4 (5), e1000074 (2008)
   PUBMED   18483556
  REMARK    GeneRIF: IRF4 and SLC24A4 loci are associated with human hair color
            and skin pigmentation.
            GeneRIF: Genome-wide association study of gene-disease association.
            (HuGE Navigator)
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 10388)
  AUTHORS   Sulem,P., Gudbjartsson,D.F., Stacey,S.N., Helgason,A., Rafnar,T.,
            Magnusson,K.P., Manolescu,A., Karason,A., Palsson,A.,
            Thorleifsson,G., Jakobsdottir,M., Steinberg,S., Palsson,S.,
            Jonasson,F., Sigurgeirsson,B., Thorisdottir,K., Ragnarsson,R.,
            Benediktsdottir,K.R., Aben,K.K., Kiemeney,L.A., Olafsson,J.H.,
            Gulcher,J., Kong,A., Thorsteinsdottir,U. and Stefansson,K.
  TITLE     Genetic determinants of hair, eye and skin pigmentation in
            Europeans
  JOURNAL   Nat Genet 39 (12), 1443-1452 (2007)
   PUBMED   17952075
  REMARK    GeneRIF: Genome-wide association study of gene-disease association.
            (HuGE Navigator)
REFERENCE   10 (bases 1 to 10388)
  AUTHORS   Li,X.F., Kraev,A.S. and Lytton,J.
  TITLE     Molecular cloning of a fourth member of the potassium-dependent
            sodium-calcium exchanger gene family, NCKX4
  JOURNAL   J Biol Chem 277 (50), 48410-48417 (2002)
   PUBMED   12379639
  REMARK    GeneRIF: identification and sequencing, as well as mapping to
            chromosomal region 14q32
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL118559.6, AF520704.1 and
            BC050357.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 26, 2020 this sequence version replaced NM_153646.3.
            
            Summary: This gene encodes a sodium/potassium/calcium exchange
            protein. The encoded antiporter transports one calcium and one
            potassium ion in exchange for four sodium ions and has been
            implicated in amelogenesis and enamel maturation. Certain variants
            in this gene have been associated with skin, hair, and eye
            pigmentation, while other variants have been identified in people
            with hypomaturation-type amelogenesis imperfecta. [provided by
            RefSeq, Nov 2023].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF520704.1, BC069653.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2145743
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000532405.6/ ENSP00000431840.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-693               AL118559.6         180821-181513       c
            694-839             AF520704.1         1-146
            840-840             AL118559.6         178767-178767       c
            841-4141            AF520704.1         148-3448
            4142-9678           AL118559.6         3907-9443           c
            9679-10388          BC050357.1         2146-2855
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.12"
     gene            1..10388
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /note="solute carrier family 24 member 4"
                     /db_xref="GeneID:123041"
                     /db_xref="HGNC:HGNC:10978"
                     /db_xref="MIM:609840"
     exon            1..796
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     CDS             667..2535
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; Na(+)/K(+)/Ca(2+)-exchange protein 4; solute
                     carrier family 24 (sodium/potassium/calcium exchanger),
                     member 4; Na/Ca-K exchanger 4"
                     /codon_start=1
                     /product="sodium/potassium/calcium exchanger 4 isoform 1
                     precursor"
                     /protein_id="NP_705932.2"
                     /db_xref="CCDS:CCDS9903.2"
                     /db_xref="GeneID:123041"
                     /db_xref="HGNC:HGNC:10978"
                     /db_xref="MIM:609840"
                     /translation="MALRGTLRPLKVRRRREMLPQQVGFVCAVLALVCCASGLFGSLG
                     HKTASASKRVLPDTWRNRKLMAPVNGTQTAKNCTDPAIHEFPTDLFSNKERQHGAVLL
                     HILGALYMFYALAIVCDDFFVPSLEKICERLHLSEDVAGATFMAAGSSTPELFASVIG
                     VFITHGDVGVGTIVGSAVFNILCIIGVCGLFAGQVVRLTWWAVCRDSVYYTISVIVLI
                     VFIYDEQIVWWEGLVLIILYVFYILIMKYNVKMQAFFTVKQKSIANGNPVNSELEAGN
                     DFYDGSYDDPSVPLLGQVKEKPQYGKNPVVMVDEIMSSSPPKFTFPEAGLRIMITNKF
                     GPRTRLRMASRIIINERQRLINSANGVSSKPLQNGRHENIENGNVPVENPEDPQQNQE
                     QQPPPQPPPPEPEPVEADFLSPFSVPEARGDKVKWVFTWPLIFLLCVTIPNCSKPRWE
                     KFFMVTFITATLWIAVFSYIMVWLVTIIGYTLGIPDVIMGITFLAAGTSVPDCMASLI
                     VARQGLGDMAVSNTIGSNVFDILVGLGVPWGLQTMVVNYGSTVKINSRGLVYSVVLLL
                     GSVALTVLGIHLNKWRLDRKLGVYVLVLYAIFLCFSIMIEFNVFTFVNLPMCREDD"
     exon            797..907
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     exon            908..984
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1059
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1144
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1248
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1323
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1349
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1403
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1546
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..1716
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1717..1921
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1922..2088
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2089..2203
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2204..2316
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2317..2382
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2383..10388
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5142..5147
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /note="hexamer: ATTAAA"
     polyA_site      5165
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
     regulatory      10369..10374
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /note="hexamer: AATAAA"
     polyA_site      10388
                     /gene="SLC24A4"
                     /gene_synonym="AI2A5; NCKX4; SHEP6; SLC24A2"
                     /note="major polyA site"
ORIGIN      
        1 agacgctggc gcccgcagcc gagaagccag agagaaagtt cccggggaga gctcgcccct
       61 gggagggccg acgtcgagcc tgctcgccgc gagggtctca ggtaccgcgc gtccagccag
      121 ccttcccgcg gcgcgcactc ggccgcccgg gctcggctcg tcgggaagca ggggaacatc
      181 gccggctggc agccggggcg gccgccgcca aacttggagg aggaggatgc tgcgagtggg
      241 cgcggcggcg cggggcgcgg gggcgcactg agtgctccct acggcgctgc tgccagggct
      301 gtggccgggc gagccggccg aagcggagcg ggcaggtagc ggctctagcg ccgggactgc
      361 gccagccctg cgagcccggg ccgccagcgc caccgtgccg gcgtcgcctc ctcgccacgg
      421 agccatggtg cgcgcagcgc gcacgcggcg cgcgggactc tgagctccgg ccgcgtcgcg
      481 cgtccccacc ttcccaaggg gctcccccgc cgacctcgcc ctcgggccat gaggctttgg
      541 cccggagctc ctcgcctctg agtcgcgcac cgcctgctcc agccccagcg ccgctcggcc
      601 actgattgca ctctggccgc tgaagctccc catcctctcc cagagacggc acccaggcgc
      661 tccgggatgg cgctccgcgg gaccctccgg ccgctcaaag ttcgcaggag gcgagagatg
      721 ctgccgcagc aagtcggctt cgtgtgcgcg gtgctggccc tggtgtgctg tgcgtccggc
      781 ctcttcggca gcttggggca caaaacagct tctgctagca aacgtgtcct gccagacacg
      841 tggagaaata gaaagttgat ggccccagtg aatgggacac agacagccaa gaactgcaca
      901 gatcctgcga ttcacgagtt ccccacagat ctgttctcca ataaggagcg acagcacgga
      961 gccgtcctgc tgcacatcct tggtgctctg tatatgttct atgccttggc catagtgtgc
     1021 gatgacttct ttgttccgtc tctagagaag atctgtgaga gactccatct gagcgaagat
     1081 gtggctggag ccaccttcat ggctgcagga agctcaacgc cagagctgtt tgcgtctgtt
     1141 attggggtgt tcatcaccca tggggacgtc ggggtgggca ccatcgtggg ctctgctgtg
     1201 ttcaacatcc tgtgcataat tggagtgtgc ggactgtttg ctggccaggt ggtccgtctg
     1261 acgtggtggg ccgtgtgccg agactccgtg tactacacca tctctgtcat cgtgctcatc
     1321 gtgttcatat atgatgaaca aattgtgtgg tgggaaggcc tggtgctcat catcttgtat
     1381 gtgttttata ttctgatcat gaagtacaat gtgaagatgc aagccttttt cacagtcaaa
     1441 caaaagagca ttgcaaacgg taacccggtc aacagtgagc tggaggctgg taatgatttc
     1501 tatgacggta gctatgatga cccttccgtg ccattgctgg ggcaagtgaa ggagaagcca
     1561 cagtatggca agaaccccgt ggtgatggtg gacgagatta tgagctccag ccctcccaag
     1621 ttcaccttcc ctgaagcagg cttacgaatc atgatcacca ataagtttgg acccaggacc
     1681 cgactacgga tggccagcag gatcatcatt aatgagcggc agagactgat caactcggcc
     1741 aatggtgtga gcagtaagcc gcttcaaaac gggaggcacg agaacattga gaacgggaat
     1801 gttcctgtgg aaaaccccga agaccctcag cagaatcagg agcagcagcc gccgccacag
     1861 ccaccaccgc cagagccaga gccggtggag gctgacttcc tgtccccctt ctccgtgccg
     1921 gaggccagag gggacaaggt caagtgggtg ttcacctggc ccctcatctt cctcctgtgc
     1981 gtcaccattc ccaactgcag caagccccgc tgggagaagt tcttcatggt caccttcatc
     2041 accgccacgc tgtggatcgc tgtgttctcc tacatcatgg tgtggctggt gactattatc
     2101 ggatacacac ttgggatccc ggatgtcatc atgggcatta ctttcctggc agcagggaca
     2161 agtgttccag actgcatggc cagcctaatt gtggcgagac aaggccttgg ggacatggca
     2221 gtctccaaca ccataggaag caacgtgttt gacatcctgg taggacttgg tgtaccgtgg
     2281 ggcctgcaga ccatggttgt taattatgga tcaacagtga agatcaacag ccgggggctg
     2341 gtctattccg tggtcctgtt gctgggctct gtcgctctca ccgtcctcgg catccaccta
     2401 aacaagtggc gactggaccg gaagctgggt gtctacgtgc tggttctcta cgccatcttc
     2461 ttgtgcttct ccataatgat agagtttaac gtctttacct tcgtcaactt gccgatgtgc
     2521 cgggaagacg attagcgctg agtcgcggcc cctgggagct gatctggaca ccctgtgaca
     2581 ctggcgttct cctctcccct ccttccccca ccacaggtct ctcctgcata ggcagccact
     2641 gtccgttctt tcacacactg gaaggaagag ccatcgtggt ctttgtctgg ccacaggcca
     2701 ggctgctggg catcctcctc ctccttggag ttccgcccct gcaaggctgg atttgggggc
     2761 cattatctga gcagcttcaa agacccctga gctgccaacc acggagatgt gccaagcatc
     2821 tcatctctcc tgcacacttt agtcagaagg acttctgcat gcagtttgtc tttctgttct
     2881 gcaggcagct tcagaattga ggtcatttgt gagcacaaga tctcataggg caggtgcaaa
     2941 ataggaatgt tgttctcaag tgtcacctcc agcccagagg tggttcctta ggcagcatgt
     3001 gctcctggga gcctctgact tttgctggaa gcagccacag tttggaaggg gcaagacctc
     3061 aacctgttgg ggtttagggc ccatgatggc agacattcta ccccttttcc tggaaaaact
     3121 ggaagaatga aaataatttt tttctgtgga agagagaaaa tgagtgaata ttcttctcac
     3181 ttttattgat gcattcagag aataagcaat gaaatattaa aaaatgaaac atcatatagg
     3241 tcatcatact tgaaaattat cattccatat gaaaggatca tgatacacac caaaaaagta
     3301 atgatcgtaa agacacaaat cctctgtatg ccatcttgca ttggcactga ggtgtttggt
     3361 ttggaatagg gaaaaaggta agagactaac gtggaaaggt gctaactcag agactggaga
     3421 ttatagttta cagctgtact ttccagatct tctatgtgac acaatgcact gtccttgtgg
     3481 gtttgtcatt tattggttaa tgctctagtt tcaaaaccac cctgttgaaa gttccagtta
     3541 tttatatgcc caacaaattt catagcctgc tgaactgaac tgagtgtgtc agaagtgctg
     3601 gttaatgacg agaagagatt gcctgaaaaa caacaaactg ctttctggtt agctgaaggc
     3661 aagtgtgaaa atcagaattt agaatattta gagctaagct tctggaacca cgtagtttct
     3721 acacgtggca ggccaagaat gggaggctga ctcaaaacta gatagaaaaa tataaaataa
     3781 tcttcgacca cttgatagct ctcaaatata tatttaaaag atttatgaat acaaaccatt
     3841 tatggtttat gatttctaaa aagaaagcac aattaatttt atagagaggt tttttatttt
     3901 tttaatattt ctattgcaaa agtctatccg atttgatgca ctttgaatat tgagatattt
     3961 tgcacggatg aatgtatggg aactacccat gatgatgtaa gaggaaagaa catttttttg
     4021 tgattcacca gacatcactt taaacttggt gatgagttta aatccagtag ctaatccctt
     4081 cctgagactc aaagatcgtg acgctggttg gaatttctga ctgtgccctt tagggcctcc
     4141 tgagtttcaa aaggaggaag tgttcgtgct tgtgtccctg aagttccctg ttgcatgagc
     4201 ctgcgacagg acctcacccc caccaccagg cttctatttg ggattcacat cagtattagt
     4261 atcgtagcta caccaagttc aggcttctct ttttgttttt ttacctagaa attgggctca
     4321 gtggtcttca acttgaggac gagggtgatt ttcctaagaa atcagcaaag agggaaggca
     4381 gggcccctgt agattcacca gtataaactt cagctgcagg gattccagag ccctcgggac
     4441 cactctgtca ccttaatagc caagttctcc tggttcctcc gatcttacag gctcatccag
     4501 gttccaaagt gcttctgtct ctgttttgat tctccaaact gctctgtgat gtatgtaggg
     4561 attattctcc ccacttaaca gaaagtagtg tcttggagag gtcaagggtc tctagttcaa
     4621 tggccagtca tagcagaagg gaggccaagc accagtccat cacccctccc aggccagcct
     4681 ctgtaagttg gccacacttg gggagtgagt gtgggtatga ctttaccctc ctggttggtt
     4741 cttactgttt gagtcaaaac ctcatcaata tatcattgac tcctgggttc ctcaggtcat
     4801 ttcctaatat ctgtccctat ccaatgcctc tattttatct tgaaaaaagg accaaaaatt
     4861 atttttagct atggcaaggc acaggccaca tggcccctga tggcgtccct gctggttttc
     4921 aattctctga agccttgtgt agctttcaga gcacacgtat cctaattacc ctcctcttcc
     4981 tcagcagaac ccatttgaga ttctaaatga atactcttag tctctaaagt tgcagttaga
     5041 aactaaaata atgtttttta atatgtaata tgctcctctt ggctaatttt cttttgactt
     5101 taatgtgcca atgtaacttc ctttaaagga tctatgcatt tattaaatct ggaaaactat
     5161 atgtacactg taggtggaaa attctctttt ttaactaaat atttttccat cacaaattta
     5221 aagaattgca tgattaatta ggctttcatt tttaaattac gctttcatca ctacgcagga
     5281 ttactttatt ttattcccaa agctcattag catgggataa ttactctgct acagaaatag
     5341 gcaatttaaa aaaatgaatt tagctcttct cattgggggc agaaaagaaa aaaaaaacca
     5401 ttgcactcag atggaaaatg cctatagaca caggagcagg tggttcctgt ggacttctgg
     5461 tttggaattt tgcctcacca ggtcaagcgt ggttagggtg gaaggtgtcc agtatcttga
     5521 aaacctggcc ctggaggaag gttctgggtc agctgcaatg agagactggt gattaagggc
     5581 accgtgggca ggacacagtc ctcgccttac ccaccccatc cttcctgtta cccacagtct
     5641 gctggcctcc atgcctcttc cccttgtcac ttgtgtctcc tccttatgca cagagctgcc
     5701 tgcctttatg aattttcttt tctttttttt ttgagacagc gtcttgctgt gtcacccagg
     5761 ctggagtgca gtggtgccat cttggctcac tgcaacctcc gcctcccagg ttcaagcaat
     5821 tcttgtgcct cagcctcctg aggattacag gcgtgcgcca ccacacccag ctaatttttg
     5881 tatttttagt agagacgggt tttcaccatg ttggccaggc tggtctcaaa ctcctgacct
     5941 caggtgatcc acccacctcg gtttcccaaa gtgctgggat tacaggtgtg agccaccacg
     6001 cccagcctgc ccttgtgaat tttcacctgc tccttacccc tcacctgtta ggactgtttc
     6061 ttgcttttgc ccctgtcggt cccctgcctt aacagaccta agcagctgat aatgcaccaa
     6121 gcttccctga ccaggtgggg tgtgtctatc acccaagggc agtcctacag accctgacca
     6181 aaggccgttc ctgggcggcc caaggtccag gtttcttcca cctgctcttc cctgtttatg
     6241 gggatttgca agcctaattg catcagcagg agcccatctc tcagagaacc cggactcccc
     6301 aagcagactg ggattttggg aagggtgtgg ggggtgtcat tgctggatac ccgtctttct
     6361 gcctgtcctt tctcctctct gaatcctggg gcccctctcc ctccttaaag ctggagtgga
     6421 cagagggaca ggagaggatc agagttcatc ccccctggga aagagcaaga gcgaatgaat
     6481 cccagcgcca gcggctgagg ctgccttccg tgccttccct ccatgggcga cgggtgagtg
     6541 gggcttagga aactggaaca gggaaggttc tgttaccaca ctttggaact ttccccctgg
     6601 gattcagcag ttgagaagca gagacctttc tgccctgggt gaatgggtcc ttgggggagg
     6661 ggttggtctt ttgtctcgca tccccatctt tcctttcctt ctgggccatg ctcctccctg
     6721 gctggaaaaa ggtggctgtg ctgtccctgt gatccactct cagcaaatgc gtgtggctca
     6781 aataaacaaa gaacttacct gttagagtga aaatcctcag gagattgtac ccaaatgcca
     6841 tgctctaaat attcatggtc tctctaatgc cctcaagacg tgatttccat gggaaccatc
     6901 ctcccctggg ggcagttagc aggagtacgt ggggcacgtg aggtggtcct cctttcagca
     6961 caccgtgccc atagaaactt ctagaaattt ctgaaaatgc tctgtgggca gctcttgggt
     7021 ggcagtaagt ccatcaaccc ccatctaccc cgggcctgaa gcgctgcgct tgctctcttt
     7081 atgtgtgtgc acccgaagga tttcctggtc tctgtagctg atcctgtgag cccctcaagc
     7141 atgaagcctc ccttggggct tctcaaagca tggagagggg cccttcctgt cctttgggaa
     7201 aatcttcccc actgtgtcag ttatatggga acaagagtga tggggtcttt ctctaggcct
     7261 gtgccacagg acagagaaca cgggattctg ctgttcgctt tgagccacag cctttaccag
     7321 cccggcttgt gtggggggcc ccttcgcctt gctgcaaaga gctgttcccc aaagggcata
     7381 tccacagggt acaggtttta aaaaggcttt tttttttttt tttgagacag ggtctcgctc
     7441 tgtcgcctag actcagtgca gtggcgccat gttggctggt tgcaacctcc acctcctggg
     7501 ttcaagtgat tctcccacct cagcctctct ggtagctggg actacaggca cgcgccacca
     7561 tgcccagcta atttttggat ttttagtaga gaaggagttt caccatgctg accaggctgg
     7621 tttcgaactc ctgacctcaa gtgatccgcc cgcctgggcc tcccagagtg ctgagattac
     7681 aggcgtgagc caccgcacct ggccaaaaaa aggcattttg atttaggttg ctgtgtttgc
     7741 ttgttgataa agaaaactca atcgggacac tagttttgtg ctcagcttta ggccgggtag
     7801 ctaatgggag gatgtccagc ctgtcactgt gctcccagcg caaggaaatg ggtgcccacc
     7861 tggaatcagg agaagaggct tttccctcct gttctgcaac cagggtggag ctatctttcc
     7921 agggaagcca gctgagaggt tttagggctt tggttatttt atgggggttt taaacctcct
     7981 aacttttcaa tgacaaatgg ctcccaggtg ccatagtctc tgttaaatcc tcaaacattc
     8041 acaagcacac actgccaggg gcacgggttg tctttcacct gcatgtttct aaggctcttt
     8101 attcaatctc acggtgtcag tgtccagttg tcaaagttat gaatcttcct cctgcttcta
     8161 aacagggctg acagtatact ctcgtctagt ctaggaacat gtctgctgct gggataccct
     8221 ggtaccagga tttgagggcc acgggtggca tctctgagag ctgaaaatcc acagagtgcc
     8281 tgtgggaaag ccaagccctt ggctgtgtgg cttttctatc ccttggattt acaggtctgg
     8341 gaattggctg cttcttagtt ataaccccag tgacaaatgc tggcttaagc cacacctgtt
     8401 cccactgttg ctagaattca aacagttgct tttttttttt ctttttgaga aagggcctca
     8461 ctctgttgcc caggctggag tgcagtggct tgatcacagc tcacgaaagc ctcaaactcc
     8521 taggctcaag tgatcctcct gaaaagtagg taggactaca ggcacatgcc accacataca
     8581 gctaatttgt tttcattttt ttttttttta gagacaggat ctcgctgtgt tccccaggta
     8641 ggtcttgaac tcctggcctc aagtgatcct cctgccttga cctcccaaag tgctggatta
     8701 caagcgtgag cccctgcacc cggcccaagc agttgcttct ttttttctct tttttttttt
     8761 ttttgagatg gagcctcact ctgttgccca ggctggagtg cagtggcgcg atctccactc
     8821 actgcaagct ccgcctcccg ggttcatgcc attctcctgc ctcagcctcc cgagtagctg
     8881 ggactacagg cgcctgccac cacacccagc taattttttg tatttttggt acagacaggg
     8941 tttcaccgtg ttagccagga tggtcttgat ctcctgatct cgtgatccgc ccaccccggc
     9001 ctcccaaagt gctggattac aagcgtgagc caccgcgccc cgccaagcag ttgcttctta
     9061 tgcaacatgt tggttgggac ttgtccacgg gccaggccaa taaaattctt aatcctgcag
     9121 agagtcagta ccctcatcac cccatcactg gaaaacaaat gttttaagct atcaagagag
     9181 ggaatgtgca gcttttggtt tctagatgca tggtttggtg tgatctacct ttgtgcctaa
     9241 agggaatgtc ccaaacaaca gagccttctt tgctgtcact ccagaattct ctacacagaa
     9301 tttcccaagt ccattcagga cagacgcgca gtcctctttc aatggaagaa gagaggactt
     9361 ttcccctcct gaaaaatgac tggagtgtga acaaggcagc tctgtttttc taaataagtt
     9421 gttcttgtga gttttttctg gccactgggc atctctgccc tcacttttca tccctgccct
     9481 ctaagctgca gaccccatga ccacactgtc tgcttccttg agcttcccgc acgaggcttg
     9541 gacctggggg acctggagac cctgcggaca gaactgtggc tgagccactg tggccaactc
     9601 ttggggagct ccacagtggg ggttgctggt ctgtgaggct gagtctccat ttcagagcac
     9661 acactccctg gcagggcgcc tctgcctgtg tctcctgccc agcagccgcc agcagggaat
     9721 agttgctggt gtctgagcac aaagagagct ttgattacct agagaggaaa aaggctgtca
     9781 gccagatgca gccaggccca ggggtagata caggagttgc taaggaaggg gccgagccag
     9841 gagaggccag gcagatccac aaagcccaag gggatgcagg ctgggtgtgg tttctgaggg
     9901 aacctaccaa atagcaggta gatggaatca gaggactctt gtgtcctgaa agaacctcct
     9961 taaaaacaac taaaacgaag aacttctggg gctgttcaca cattgttcaa gtcaccccaa
    10021 gatcgttctg gcacgctgag ctgaacacca ccatctttgt tcattctctc tctaatgggc
    10081 aaagcaggat catcgagttg aaaagttgta aataatgagg atatttatcc cgctatttat
    10141 tttttcaata actgtgacct cctgcactgt gaatgctctg tgacatgaga ttcttagttt
    10201 aataaaactg tcattaaatt tgaatgaatt gatattattg gttactgaac actggcatga
    10261 gtttattttt attgtgaaga aaaaaatcta cagcaatcta aactaaacct ttctaagaaa
    10321 tctagcagtc agtattgtaa tgcaatatat caaaatctgt acactgtcaa taaaataaat
    10381 gagcacaa
//
