LOCUS       NM_020344              10990 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens solute carrier family 24 member 2 (SLC24A2),
            transcript variant 1, mRNA.
ACCESSION   NM_020344 XM_005251426
VERSION     NM_020344.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   Bian,Q., Li,H., Wang,X., Liang,T. and Zhang,K.
  TITLE     Multiomics Integrated Analysis Identifies SLC24A2 as a Potential
            Link between Type 2 Diabetes and Cancer
  JOURNAL   J Diabetes Res 2022, 4629419 (2022)
   PUBMED   35601016
  REMARK    GeneRIF: Multiomics Integrated Analysis Identifies SLC24A2 as a
            Potential Link between Type 2 Diabetes and Cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10990)
  AUTHORS   Wang,F., Luo,Q., Chen,Y., Liu,Y., Xu,K., Adhikari,K., Cai,X.,
            Liu,J., Li,Y., Liu,X., Ramirez-Aristeguieta,L.M., Yuan,Z., Zhou,Y.,
            Li,F.F., Jiang,B., Jin,L., Ruiz-Linares,A., Yang,Z., Liu,F. and
            Wang,S.
  TITLE     A Genome-Wide Scan on Individual Typology Angle Found Variants at
            SLC24A2 Associated with Skin Color Variation in Chinese Populations
  JOURNAL   J Invest Dermatol 142 (4), 1223-1227 (2022)
   PUBMED   34570997
  REMARK    GeneRIF: A Genome-Wide Scan on Individual Typology Angle Found
            Variants at SLC24A2 Associated with Skin Color Variation in Chinese
            Populations.
REFERENCE   3  (bases 1 to 10990)
  AUTHORS   Jalloul,A.H., Liu,G., Szerencsei,R.T. and Schnetkamp,P.P.M.
  TITLE     Residues important for Ca2+ ion transport in the neuronal
            K+-dependent Na+-Ca2+ exchanger (NCKX2)
  JOURNAL   Cell Calcium 74, 187-197 (2018)
   PUBMED   30173760
  REMARK    GeneRIF: residues in the two regions with the highest degree of
            homology between the different NCKX isoforms (alpha-1 and alpha-2
            repeats) were examined to determine which residues are important
            for Ca(2+) coordination.
REFERENCE   4  (bases 1 to 10990)
  AUTHORS   Wang,L., Shao,Z., Chen,S., Shi,L. and Li,Z.
  TITLE     A SLC24A2 Gene Variant Uncovered in Pancreatic Ductal
            Adenocarcinoma by Whole Exome Sequencing
  JOURNAL   Tohoku J Exp Med 241 (4), 287-295 (2017)
   PUBMED   28413183
  REMARK    GeneRIF: a new mutation in SLC24A2 gene was identified to decrease
            the stability of SLC24A2
REFERENCE   5  (bases 1 to 10990)
  AUTHORS   Cuomo,O., Pignataro,G., Gala,R., Boscia,F., Tortiglione,A.,
            Molinaro,P., Di Renzo,G., Lytton,J. and Annunziato,L.
  TITLE     Involvement of the potassium-dependent sodium/calcium exchanger
            gene product NCKX2 in the brain insult induced by permanent focal
            cerebral ischemia
  JOURNAL   Ann N Y Acad Sci 1099, 486-489 (2007)
   PUBMED   17446491
REFERENCE   6  (bases 1 to 10990)
  AUTHORS   Kang,K.J., Kinjo,T.G., Szerencsei,R.T. and Schnetkamp,P.P.
  TITLE     Residues contributing to the Ca2+ and K+ binding pocket of the
            NCKX2 Na+/Ca2+-K+ exchanger
  JOURNAL   J Biol Chem 280 (8), 6823-6833 (2005)
   PUBMED   15583008
  REMARK    GeneRIF: Glu(188) and Asp(548) are the central residues of a single
            cation binding pocket that can accommodate both K(+) and Ca(2+).
            Furthermore, a single set of residues lines a transport pathway for
            both K(+) and Ca(2+).
REFERENCE   7  (bases 1 to 10990)
  AUTHORS   Kinjo,T.G., Szerencsei,R.T., Winkfein,R.J. and Schnetkamp,P.P.
  TITLE     Role of cysteine residues in the NCKX2 Na+/Ca(2+)-K+ Exchanger:
            generation of a functional cysteine-free exchanger
  JOURNAL   Biochemistry 43 (24), 7940-7947 (2004)
   PUBMED   15196038
  REMARK    GeneRIF: results appear to rule out a critical importance of an
            intramolecular disulfide linkage in NCKX2 protein synthesis and
            folding as had been reported before
REFERENCE   8  (bases 1 to 10990)
  AUTHORS   Winkfein,R.J., Szerencsei,R.T., Kinjo,T.G., Kang,K., Perizzolo,M.,
            Eisner,L. and Schnetkamp,P.P.
  TITLE     Scanning mutagenesis of the alpha repeats and of the transmembrane
            acidic residues of the human retinal cone Na/Ca-K exchanger
  JOURNAL   Biochemistry 42 (2), 543-552 (2003)
   PUBMED   12525183
  REMARK    GeneRIF: This is the first systematic NCKX mutagenesis study in
            which 96 residues are mutated in the human cone NCKX2 cDNA, and
            functional consequences of these mutations are measured.
REFERENCE   9  (bases 1 to 10990)
  AUTHORS   Yoo,S.S., Leach,S. and Lytton,J.
  TITLE     Studies on the oligomeric state of the sodium/calcium + potassium
            exchanger NCKX2
  JOURNAL   Ann N Y Acad Sci 976, 94-96 (2002)
   PUBMED   12502543
  REMARK    GeneRIF: The oligomeric state of this protein is studied.
REFERENCE   10 (bases 1 to 10990)
  AUTHORS   Prinsen,C.F., Szerencsei,R.T. and Schnetkamp,P.P.
  TITLE     Molecular cloning and functional expression of the
            potassium-dependent sodium-calcium exchanger from human and chicken
            retinal cone photoreceptors
  JOURNAL   J Neurosci 20 (4), 1424-1434 (2000)
   PUBMED   10662833
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL158077.15, AL133281.12 and
            AL158206.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On or before Nov 5, 2019 this sequence version replaced
            XM_005251426.4, NM_020344.3.
            
            Summary: This gene encodes a member of the calcium/cation
            antiporter superfamily of transport proteins. The encoded protein
            belongs to the SLC24 branch of exchangers, which can mediate the
            extrusion of one Ca2+ ion and one K+ ion in exchange for four Na+
            ions. This family member is a retinal cone/brain exchanger that can
            mediate a light-induced decrease in free Ca2+ concentration. This
            protein may also play a neuroprotective role during ischemic brain
            injury. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1). Variants 1 and 3
            encode the same isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.103265.1,
                                           SRR1803614.280392.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000341998.7/ ENSP00000344801.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-153               AL158077.15        35626-35778         c
            154-1236            AL158077.15        32678-33760         c
            1237-1275           AL133281.12        25699-25737         c
            1276-1384           AL133281.12        23022-23130         c
            1385-1435           AL133281.12        667-717             c
            1436-1534           AL158206.8         143804-143902       c
            1535-1653           AL158206.8         140231-140349       c
            1654-1785           AL158206.8         117017-117148       c
            1786-1875           AL158206.8         94929-95018         c
            1876-2042           AL158206.8         87774-87940         c
            2043-10990          AL158206.8         74335-83282         c
FEATURES             Location/Qualifiers
     source          1..10990
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p22.1-p21.3"
     gene            1..10990
                     /gene="SLC24A2"
                     /gene_synonym="NCKX2"
                     /note="solute carrier family 24 member 2"
                     /db_xref="GeneID:25769"
                     /db_xref="HGNC:HGNC:10976"
                     /db_xref="MIM:609838"
     exon            1..153
                     /gene="SLC24A2"
                     /gene_synonym="NCKX2"
                     /inference="alignment:Splign:2.1.0"
     exon            154..1236
                     /gene="SLC24A2"
                     /gene_synonym="NCKX2"
                     /inference="alignment:Splign:2.1.0"
     CDS             307..2292
                     /gene="SLC24A2"
                     /gene_synonym="NCKX2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     retinal cone Na-Ca+K exchanger; Na(+)/K(+)/Ca(2+)-exchange
                     protein 2; solute carrier family 24
                     (sodium/potassium/calcium exchanger), member 2; Na/Ca-K
                     exchanger 2"
                     /codon_start=1
                     /product="sodium/potassium/calcium exchanger 2 isoform 1"
                     /protein_id="NP_065077.1"
                     /db_xref="CCDS:CCDS6493.1"
                     /db_xref="GeneID:25769"
                     /db_xref="HGNC:HGNC:10976"
                     /db_xref="MIM:609838"
                     /translation="MDLQQSTTITSLEKWCLDESLSGCRRHYSVKKKLKLIRVLGLFM
                     GLVAISTVSFSISAFSETDTQSTGEASVVSGPRVAQGYHQRTLLDLNDKILDYTPQPP
                     LSKEGESENSTDHAQGDYPKDIFSLEERRKGAIILHVIGMIYMFIALAIVCDEFFVPS
                     LTVITEKLGISDDVAGATFMAAGGSAPELFTSLIGVFIAHSNVGIGTIVGSAVFNILF
                     VIGMCALFSREILNLTWWPLFRDVSFYIVDLIMLIIFFLDNVIMWWESLLLLTAYFCY
                     VVFMKFNVQVEKWVKQMINRNKVVKVTAPEAQAKPSAARDKDEPTLPAKPRLQRGGSS
                     ASLHNSLMRNSIFQLMIHTLDPLAEELGSYGKLKYYDTMTEEGRFREKASILHKIAKK
                     KCHVDENERQNGAANHVEKIELPNSTSTDVEMTPSSDASEPVQNGNLSHNIEGAEAQT
                     ADEEEDQPLSLAWPSETRKQVTFLIVFPIVFPLWITLPDVRKPSSRKFFPITFFGSIT
                     WIAVFSYLMVWWAHQVGETIGISEEIMGLTILAAGTSIPDLITSVIVARKGLGDMAVS
                     SSVGSNIFDITVGLPLPWLLYTVIHRFQPVAVSSNGLFCAIVLLFIMLLFVILSIALC
                     KWRMNKILGFIMFGLYFVFLVVSVLLEDRILTCPVSI"
     exon            1237..1275
                     /gene="SLC24A2"
                     /gene_synonym="NCKX2"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1384
                     /gene="SLC24A2"
                     /gene_synonym="NCKX2"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1435
                     /gene="SLC24A2"
                     /gene_synonym="NCKX2"
                     /inference="alignment:Splign:2.1.0"
     exon            1436..1534
                     /gene="SLC24A2"
                     /gene_synonym="NCKX2"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1653
                     /gene="SLC24A2"
                     /gene_synonym="NCKX2"
                     /inference="alignment:Splign:2.1.0"
     exon            1654..1785
                     /gene="SLC24A2"
                     /gene_synonym="NCKX2"
                     /inference="alignment:Splign:2.1.0"
     exon            1786..1875
                     /gene="SLC24A2"
                     /gene_synonym="NCKX2"
                     /inference="alignment:Splign:2.1.0"
     exon            1876..2042
                     /gene="SLC24A2"
                     /gene_synonym="NCKX2"
                     /inference="alignment:Splign:2.1.0"
     exon            2043..10990
                     /gene="SLC24A2"
                     /gene_synonym="NCKX2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10968..10973
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC24A2"
                     /gene_synonym="NCKX2"
                     /note="hexamer: AATAAA"
     polyA_site      10990
                     /gene="SLC24A2"
                     /gene_synonym="NCKX2"
                     /note="major polyA site"
ORIGIN      
        1 agtctcctcc cgaacccagc ccggctggcg agagcggcag ccagtgcgag ccagagcccg
       61 ggcctcagag cgggtaggcg gagggccggc ggactggagc ggcgggccgc gctcgggggc
      121 cccgccgtgt gcgcgtcctc ccatcttatc ctgcataaat gatgaaagtt tcctgtgaag
      181 cataacaatc cctgattgtg aaagtacccc acaagcatcg ttaactataa agtaaggaaa
      241 agtatgaaag aaccatctgg ttgagtctaa agttggaaga tcaccatatc caccagaaga
      301 cccaggatgg atctgcaaca aagcaccacc atcacttccc tagagaaatg gtgtttggat
      361 gagtcactgt ctggctgcag aagacattat agtgtcaaga aaaaactgaa gttaattcga
      421 gtcttaggcc ttttcatggg tctggtagcc attagcactg tctcattttc aatcagtgcc
      481 ttttctgaga cagatacaca gagcacagga gaggccagtg ttgtaagtgg ccctagggta
      541 gcacagggtt accatcagag aactctctta gatttaaatg acaagattct ggattatact
      601 ccacagccac ctctttctaa ggaaggcgag tctgagaata gtacagatca cgcccaagga
      661 gactacccga aagacatctt ttcccttgag gagagaagaa aaggtgcgat cattctgcat
      721 gtcattggaa tgatctacat gttcatagcc ttagccattg tctgtgatga gttctttgtt
      781 ccttctttga ctgtcatcac tgaaaaactg ggcatctctg atgatgtggc tggagccacc
      841 ttcatggctg caggagggtc agccccagaa cttttcacat ctctcatagg ggtatttatc
      901 gctcacagca acgttggcat aggcacaatt gtaggttcag cagtattcaa catcctcttt
      961 gttattggca tgtgtgctct gttttctaga gaaatcttaa acctgacatg gtggccgctc
     1021 tttcgagatg tgtctttcta cattgttgac ttgatcatgc tgatcatatt tttcctggat
     1081 aatgtcatca tgtggtggga aagcttgctt ctcttaacag cttatttttg ctatgtggtt
     1141 ttcatgaaat tcaacgtcca agtagaaaaa tgggtgaagc aaatgataaa ccgcaataag
     1201 gtcgtcaagg tgacagcacc agaagcccaa gcaaagccat ctgcagccag ggacaaggat
     1261 gaaccaactc taccggctaa gccgcgtctc cagcgaggtg gaagctctgc ctccctccac
     1321 aacagtctca tgaggaatag catcttccaa ctcatgatac acacccttga cccactcgcc
     1381 gaagaacttg gatcatatgg aaaactaaaa tattatgaca caatgactga agaagggagg
     1441 ttcagagaaa aggcttcaat tctccacaag atcgccaaga agaaatgtca tgtggatgag
     1501 aacgagaggc agaatggggc tgccaaccac gtggaaaaaa ttgagcttcc aaacagcacc
     1561 agcacagatg ttgaaatgac accatccagt gatgcttcag aacctgtaca aaatggaaat
     1621 ctctcccaca acattgaagg tgcagaagcc cagaccgctg atgaggagga ggaccagcct
     1681 ctcagccttg cctggccttc tgaaacccgc aagcaagtca cgtttctgat tgttttcccc
     1741 atagtgtttc ctctctggat tacgttacct gacgttcgca aaccttcatc gaggaagttt
     1801 tttcccatca cgttctttgg ctccattacc tggattgcag tattctctta cttgatggtc
     1861 tggtgggcgc accaggttgg agagacaatt ggcatcagtg aagagattat gggcctgacc
     1921 atcttggctg ctgggacctc catccctgat cttatcacca gtgtcatagt ggcccggaag
     1981 gggttagggg acatggctgt gtccagctct gttggaagca acatttttga catcactgta
     2041 gggctcccac tgccctggct cctgtacacc gtcattcaca gattccagcc agtggctgtc
     2101 agcagcaatg gccttttctg tgccatcgtc cttctcttca tcatgctgct cttcgtcatc
     2161 ctctctatcg ccctctgcaa gtggcgaatg aacaaaatcc tgggcttcat catgtttggc
     2221 ctctactttg tgttcctggt ggtgagcgtt ctcctagaag acagaattct tacatgcccc
     2281 gtctccatct agcaggaaaa gccatatctt gcaccagcag catgaatggt ccctccacac
     2341 tctgggctct gggctccttg acctcttgag aagaggcagc tggcacacag ccctgggtgc
     2401 caagtgtccc tccttggtga atttgaggag agatggattc acactgggcc cattcacttc
     2461 acaggctgct tccaccctgc ctactgaaat gacttcatgt aaaagacaaa gagaaccgtc
     2521 aatacagggc ttctccctat tcaccactga caagtacacc tcgctggtcg gaggtacagt
     2581 cgatgcaaac aaggacacag gctatatata tctataaata cacatattat aaatatacgc
     2641 acatgaacac acaaatcctg cctgttcctg tactatacct ctccttgcat acctatagat
     2701 tagtacatac ctgtaaataa agacaaagaa aaattatata tatatatata aaatcagata
     2761 tatataaaag tacaaatgca tcttttcagg gatagctcta gtatatttat agtacttatt
     2821 tgaagagggg catcaacttg cagtctgggc tttacctctt aagtacaaga ggtgaactaa
     2881 tggaaccaac aagacaaagg tagcattgct gtgaccttca gcctaccttg gacttgctgt
     2941 gatcctgcct agtaaggtag atggtttagg gaaatagctt gcagggagga gggcttagtg
     3001 gagaaggtct gtaggggccc tgagttgagc cttccaaaac attcctacaa gccaaacaga
     3061 gagactatct gcccacctcc accagagtac cctgaccaag aaactcaggg tacttaggac
     3121 agaaagaggt aaaaacccag gctgggtggc ttggtttggg gaagtgggga ggtaaccagg
     3181 gttgggttgt tttgttttgt tcttatattg tcccagcatg caagtagaaa atatgactac
     3241 aaaccaaatt agtgcttttt ctaggacagc aagactatat aaactgaacc tgtatttttc
     3301 cagcgcatgt cccattatca tcaaaagaag aggggtaagt ttagctgtga ttgtcatgta
     3361 gatatatttt gatgctaagg gctgccaaga gagcctagat gtgcaataag aagtcatcca
     3421 gtaaaggtgc ttagtgggcc ttgccaccta atgggatgta agggcatagt tctttaaaac
     3481 gttttccagt gtagtcataa agggctttag tgtctgaaga aatcattatt cccaatagca
     3541 ctaacttaac tctgatcagc actgcttggg ttgaggcaac ttagcgatgc ttggaaaggc
     3601 atttgcacac tcagtgcacc ccaggaaggt ttctgagtgc caggtcgagt ggtaccagga
     3661 ccaggctttg gttaaagttc ttgtaacatc tatgtctctt cttccatcaa gaccttatct
     3721 gggattctgt ctaagtttta gagtacacgg tatttgacag cttccttata ctggtttcaa
     3781 gctattcact ccaacaattt gatttcttga ttttgctcaa cattacattg aatgcatgaa
     3841 attaaaatat tccacgcccc caagttctgt atgacacctt gtgtgttttc tcagatctat
     3901 gtcagtcgaa aataaagcca gcaaggggga aaaaataggc aaactgaatc aatggaaaac
     3961 tatgtagagc acttttcttt tctccaagtc atgctttcct taaaatcagt tgaaaaattg
     4021 aatcaaggat ggttcattat tctgtaccca tcctacgtct aagttggtac atatggcata
     4081 tgggaaattt caccatatgt gttcctatct ccaatctatt ctgtcattga cgcctgctat
     4141 gattttgttg tgagcacata cctcttaaca taagaggaca ggatgcagaa ctcacagcag
     4201 aataagagga aaggtaacct gacagcccca gatcaagctc agtattcctt ccagaaaacc
     4261 tcagctcacc ccacagagca tggcaataac aggaaatgcc catcattcct cttcacccag
     4321 aagtaaaaag cacttcaaac ccgaggcccc ctgggaaaac aagaaatcaa gatggctgcc
     4381 agtcggaggc tggctttcaa gtctctccaa ctcggcagat tggggtcttc agatgtggga
     4441 caatgccttc caagacattg aaacaccatc caatagaggc gctgagtgga acctgccacc
     4501 taatgggaat gaatctcatt catcaaggtc aggactttgt actggggcaa gaatttgtcc
     4561 tgcttcctta gagagtgaaa gaatgaggaa gtgaacttta cgtttaaaaa ctccagttgt
     4621 tttccctgtg tatttcttga agggcaacca agttggttat tgtgtgaatt ttatattatt
     4681 gactggtgcg ttgtttgaaa taaggtttaa ataggtgact gactcaactg caaacagacc
     4741 atgtaaaaca gaagttgaag gagacctgat gccgccctct tcttattcct gatcctccct
     4801 ctgcctcctc actccctccc atttattccc agcttacagt gaaggctgat caaagcagct
     4861 atggtgccct ttgcttgtct cggagctcca cttcacagga agtgttgtgg tctttcatgt
     4921 gaagttgcat ttgagaagac aaaatttcca agccccgttc ccacatcagt catgtgcact
     4981 gagcctgacc tacaacagca ctgctccctc actggatggg acacaagtga ttccacagcc
     5041 tgcccttgct ttcccttcat ttctgtgtct gtaagtggtt atgtgttgag ccagggtaaa
     5101 ttcagagaga tgtgtacaga gttcaacaat gggtctttaa accattcaaa tcattggcat
     5161 aattataaga actttccaca ccgtggtttc caaaacaggt catctctaac tcgtttcctg
     5221 catgcctgtg tatatacata tataaatata tacatatata tatatataca tatatatata
     5281 tatatatata tctttatacc agatctatat gtacacatat aatatgactt acatcatcac
     5341 cctctgaccc tatcacattc aggattctaa agcagggagc atcaagtttg ccaaagtcaa
     5401 ggaaaaggaa agtcatgcac acaaaccaaa gggaacattt cttcctgatt tcataaggtt
     5461 gtttgttcca tgcacatata tttacaaata tacacatatg tacccatatt ttcccaccct
     5521 aagaatatca atttatagcc ttaaggaaat cggtcagcca tatccctaat gctgatatag
     5581 aggcaaagaa tacacaacag aaaatacaaa gcaaaaattt agactgcaga tactctcttt
     5641 gcttctctgg agcagggtat aaaagatatc aacaattcaa gagccaacat tgtactaatc
     5701 aatctaaaat aaatgagttg cacgcaaatg atttcattta ttcacagcaa accgagcagg
     5761 tcttgcttgc tgtcaccgcc taccctcccc catacttcca aggcagcaga gcagcttggc
     5821 tcaaactgac aattcccaaa tagaaactcc taaattgcat ctgagaatgc ccatgccaac
     5881 tccagctgcc ctctgcacct gaccagatcc tgatgaactg aagctgaaga tcagatggtg
     5941 tttatgcagc ttcccaggac ctaaactgag tcaggcagtg acagagcaat gatatatgca
     6001 gggactaagg gatagggttg tttataccct cggcagatgc agcagaatgt cctaaagcct
     6061 tgcccagaag attataaaaa gaggcggaga ctgttttata ttcaggaaaa atgctttgta
     6121 aggcttctaa caggtgtcac ctgacaaggg ccatcataat ttattttata gggaagacat
     6181 gaatcaggtg gtttggagcc ctttcagcaa aacgcagctg cctcgctgat tagacatttg
     6241 acaatgttaa tgtacatcca ttcctgggca gggagagagt tggcaaactc cccaccactg
     6301 cacatagctt ttaaaggcct ctggaacagt gagagaagag atgagctaca aagggagccc
     6361 acccccagac cactttaaca aaaagttgac caaagaaaag acacctggta gagtggctgg
     6421 ccagatagag ctgaagaaga tggaagttgc attaaatgct ctgcagaaat tactgcgttg
     6481 gactatctaa catgggtctg atggcaggca tttcagaaca tggcagtgtg ctttcctccc
     6541 cacatctacc aaacacacac ccacaccccc acacacacgc gagattatgt ggatgctatg
     6601 cctccttgca cctggacaga ttgtttttct ggtaagtagg tggccctatt ataggtggca
     6661 gctgtgttcc agaagaaaca aagtgaagct gttctcaggg tgatttctgg agaaaactac
     6721 tgtctcctga aagcatgcat agaagtcagt gctcactctt gctaagccac atattcctta
     6781 acgatccggc ttaatatttt taaaagatac ctcctcaacc cctatacctg tctcaacaat
     6841 actgtcttca attattaggt aaggccttgt cattaagaga ggggtaggag aaaaaataga
     6901 ataataagga aagggaagaa aggagatatt aatttcctag gattccaaga aaacaagtgt
     6961 cacgtggaaa tatatcccta tgtagtttaa aacagtaaca aatatcaggc tatgtttagc
     7021 caattacaag agcccatata cagttttaga tttttttaaa aaataatctc aggaagacct
     7081 tgggtaagaa aagtaaccct gccctattcc accttctatt tactgttagc tccctgactc
     7141 tgcctctgcc tctgtctttg cgtttcattt ttaatctcag agcttcatat tttttaaata
     7201 tatagcaaac aagaaaagca ataagggaac tactcacagc cagctcaact ctattactta
     7261 tgattacacc atggcaatat tcctttttca ccaggagctt tggacctgcg caggttgtgg
     7321 catgtaatca cccggagcat gtagtcgtct gtagaaatca caggcacact catgtttgct
     7381 ctggaaggaa tctgttttcc acaatgactc cccccagcta atgtacacac tggcattttg
     7441 catgccttcc tcacacatgg ggcaccagcc ttgctcagaa ccacccaaac tccacagagg
     7501 cccttaatat ggctagggac agatttctta agaaagagta agaagcagct acaaaggaca
     7561 agcaaatcca caagtcaggc agcattcgag aggcaaaaat gaccctgcaa ttacgacagg
     7621 ctaggcaact gttttcaaat tagcgtacga acagtgaggg actgtgtaga gcaccatatt
     7681 atctaagcca aagcacttct accagatcat tcattgacag actggaaaac ctaataaaat
     7741 ctttaaaaac agacaaatat ctaagtctga aagaatgcca tggagtttgc ctaggcttcc
     7801 accaaagatt taattccatt aagagggttt gtctagccta tcacgctaat ccaaaggaca
     7861 gtcctcttac gggggagatc ccagcataga tgctggtgtg acccagctca atgattaaca
     7921 accatcagca tcaagaaaag cctccctcca agttaaccca aacccatcca cttaatgact
     7981 taactttttt tttttttttt tttttttttg gcaaaaagta actgcatctg ggcactcttc
     8041 actctttgcc ccatagttgg aagttaccgc tatgtgactc tttacctatt ttcaattaaa
     8101 attatcttta gatttttatt tgagtcttta accctcttaa taatttttat agttcctctc
     8161 tactaagcct ttttaagttc tttatattgt tcttaaatga tggaacctta tgactaatga
     8221 taatcactgt ctgaacagtg ttgaactata ctgggtgcag catgaccatg aagctctggt
     8281 gaagtcaagg tcacaaactg cttttaaggt cattaggtca taatctcaga ctcacaaaga
     8341 actcaaaatt ggggagacac cactttagat tactccaaaa ctctgggcct gtacttaccc
     8401 actaatttat caagaggaat caatattttt attttaaata tgaaactggg ataaatgatt
     8461 ggatctccac ttattaacca gtactgcagt gtgtttatat ccttttgtgt ggtaggttgg
     8521 ataggatttg taaagctact gattgatctc atgggccagg gttcaccaaa aggatgggaa
     8581 tttgtttctg cattcttaca tattgggttg catattcctt cataatggat ccataagtaa
     8641 ctaaggcctt ggagactaac agttctccaa aatactcagc taaccaaaag aaaatactaa
     8701 tttttaaaat catgtcataa aaaacagaaa agtaaaaaaa gtctgaaatc ctgaataaaa
     8761 ttattttaag gatgaaatta catgcataaa ttaagggtag ataacttctt gccatttaat
     8821 taaacaaatc cccaattctt atcagaataa aactgaagga caatggatgc cttttgggtt
     8881 ttttaatttt ttaatattgc actttatagt acttcataag agacaactgt cttaattaaa
     8941 gccataaaat accaataata catatattca gggcttctga atatagtcaa tcaataagca
     9001 gtccatgagt cattcctgtt ttgtattgtt tattgtaaat tggagtttat ctgttctatt
     9061 ttttattaac tacaattatt gttctaggca atcctaatct acttatacat tttagatgtg
     9121 tgctttaata aaactgtttg aaaatcaaag aaattattca tttcacaaaa gcagtcttcg
     9181 ttttcaaaat agcttattac agtttcttta gcaagttcta atactacatg gaaattattt
     9241 taaaattgta gatttttttc aggaatccat tttgttatat aaagcaaagt tcacccatat
     9301 ggaaaatgaa atagataatt agcaaacata tacctgggaa atgtaggatg tgtttgagct
     9361 tttatataaa tataggatgg gtctaagaag ttcccttata aaaatgggaa atagaggtat
     9421 acaatttatc aataattcct tatgcctaaa ttactaacct tccttgggag gacatgtaaa
     9481 gcagcagatt tcataatgct ttggtggtta ttttcctcct ctccgctaaa atacacaatt
     9541 tctaacctag tatggtacac tgtttacaca cacatatata aagatgtccc cacaactaaa
     9601 aactttaatg tttcaacttc atcacaaatt tttatataat ttttttttta acagacaggg
     9661 tcttgctctg tcacccaggc tagagtgcag tggtgcaatc atagctcagt gtagcctcaa
     9721 acacctggac tcaagcgatc ctcccacttc agcctcttga gtagctggga cctcaggcac
     9781 atgccaccat gcctggctaa ttttgttgtt gttgttaaga gatggagtct cactatgtta
     9841 cccaggctgg tctcaaactc ctgggctcaa gagatcctcc tgctttggcc tcccaaagtg
     9901 ctgggattaa aggaatgagc caccataccc agccctttta tgtaatttga gtaaaagtaa
     9961 ttactaaaaa gcacttaata gtctagaaat ccaccagaac tgggtaccta aattgtccat
    10021 atagagtaaa catgggtact aaactggaca accccatcaa ttttatgctt aagtggccaa
    10081 attgccctaa agaaagggta tctgaaccaa gggccagtga tattcctctg ttagaggctg
    10141 ttttgtggtc tgaagagttt ctctaaaggg ctgagactct attcggaaga gggagcctca
    10201 gttactgaat tgcgctattg aaccctgaac acaaagtaca catatatacc ctgcatctag
    10261 ccttgatatt tcttctgctc aagtcaaaaa actccggaag caacagcttc caccatcaag
    10321 caatccacat acttaaaaag ccatcaaagc taagaatagc aaaagctacc ttcctgattc
    10381 agtgaagttg ctgctagcac taaaagcaag agaaattata cttttgttct tcagatacgg
    10441 ttaaaacaaa gaaaatgttg tcaacgttga cgccaacata gacatttgct ctataacata
    10501 taaactgtct tttagacagg ttatgttaaa aatatcagaa tgccattttt gtaaaggatc
    10561 ttttttagga tagacgtggt ctcatgcttc ctttgttcgc ctccgtttta aaggagcatt
    10621 gtaaccccta tcctcatccc tacaaaaatc cagtggatgc acttccaaat tacttctgtc
    10681 attttgtatg tgttgtgttt attatgcgta attttcactg gatgatgaga tacatggtga
    10741 tactatgtca gtttattctc tgtgaatatg tctgtacata gacaggaagc tctacttatt
    10801 tgtacatagg ctgtctgaag tatgtacttc tgtcacatgg acttcattct gataaagggt
    10861 gtttcttctt gtttcactgt ccataccttt gtgcttctac tactgataat caattcatag
    10921 aatatcactt tatgtaacta agtctcctca gtgtgaaaaa taatagtaat aaaattattg
    10981 actcaaagaa
//
