LOCUS       NM_004171              12006 bp    mRNA    linear   PRI 07-OCT-2024
DEFINITION  Homo sapiens solute carrier family 1 member 2 (SLC1A2), transcript
            variant 1, mRNA.
ACCESSION   NM_004171
VERSION     NM_004171.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12006)
  AUTHORS   Galli,A., Moretti,S., Dule,N., Di Cairano,E.S., Castagna,M.,
            Marciani,P., Battaglia,C., Bertuzzi,F., Fiorina,P., Pastore,I., La
            Rosa,S., Davalli,A., Folli,F. and Perego,C.
  TITLE     Hyperglycemia impairs EAAT2 glutamate transporter trafficking and
            glutamate clearance in islets of Langerhans: implications for type
            2 diabetes pathogenesis and treatment
  JOURNAL   Am J Physiol Endocrinol Metab 327 (1), E27-E41 (2024)
   PUBMED   38690938
  REMARK    GeneRIF: Hyperglycemia impairs EAAT2 glutamate transporter
            trafficking and glutamate clearance in islets of Langerhans:
            implications for type 2 diabetes pathogenesis and treatment.
REFERENCE   2  (bases 1 to 12006)
  AUTHORS   Buccoliero,A.M., Caporalini,C., Moscardi,S., Cetica,V., Mei,D.,
            Conti,V., Nozzoli,F., Bonaudo,C., Battista,F., Giordano,F.,
            Mura,R., Spacca,B., Mussa,F., D'Onofrio,V., Guerrini,R.,
            Genitori,L. and Scagnet,M.
  TITLE     Leat-associated seizures the possible role of EAAT2, pyruvate
            carboxylase and glutamine synthetase
  JOURNAL   Epilepsy Res 199, 107258 (2024)
   PUBMED   38086219
  REMARK    GeneRIF: Leat-associated seizures the possible role of EAAT2,
            pyruvate carboxylase and glutamine synthetase.
REFERENCE   3  (bases 1 to 12006)
  AUTHORS   Yesiloren,E. and Yalcin,G.D.
  TITLE     The Regulation of GLT-1 Degradation Pathway by SIRT4
  JOURNAL   Neurochem Res 48 (9), 2847-2856 (2023)
   PUBMED   37178383
  REMARK    GeneRIF: The Regulation of GLT-1 Degradation Pathway by SIRT4.
REFERENCE   4  (bases 1 to 12006)
  AUTHORS   Alijanpour,S., Miryounesi,M. and Ghafouri-Fard,S.
  TITLE     The role of excitatory amino acid transporter 2 (EAAT2) in epilepsy
            and other neurological disorders
  JOURNAL   Metab Brain Dis 38 (1), 1-16 (2023)
   PUBMED   36173507
  REMARK    GeneRIF: The role of excitatory amino acid transporter 2 (EAAT2) in
            epilepsy and other neurological disorders.
            Review article
REFERENCE   5  (bases 1 to 12006)
  AUTHORS   Kato,T., Kusakizako,T., Jin,C., Zhou,X., Ohgaki,R., Quan,L., Xu,M.,
            Okuda,S., Kobayashi,K., Yamashita,K., Nishizawa,T., Kanai,Y. and
            Nureki,O.
  TITLE     Structural insights into inhibitory mechanism of human excitatory
            amino acid transporter EAAT2
  JOURNAL   Nat Commun 13 (1), 4714 (2022)
   PUBMED   35953475
  REMARK    GeneRIF: Structural insights into inhibitory mechanism of human
            excitatory amino acid transporter EAAT2.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 12006)
  AUTHORS   Meyer,T., Speer,A., Meyer,B., Sitte,W., Kuther,G. and Ludolph,A.C.
  TITLE     The glial glutamate transporter complementary DNA in patients with
            amyotrophic lateral sclerosis
  JOURNAL   Ann Neurol 40 (3), 456-459 (1996)
   PUBMED   8797537
REFERENCE   7  (bases 1 to 12006)
  AUTHORS   Li,X. and Francke,U.
  TITLE     Assignment of the gene SLC1A2 coding for the human glutamate
            transporter EAAT2 to human chromosome 11 bands p13-p12
  JOURNAL   Cytogenet Cell Genet 71 (3), 212-213 (1995)
   PUBMED   7587378
REFERENCE   8  (bases 1 to 12006)
  AUTHORS   Manfras,B.J., Rudert,W.A., Trucco,M. and Boehm,B.O.
  TITLE     Cloning and characterization of a glutamate transporter cDNA from
            human brain and pancreas
  JOURNAL   Biochim Biophys Acta 1195 (1), 185-188 (1994)
   PUBMED   7522567
REFERENCE   9  (bases 1 to 12006)
  AUTHORS   Arriza,J.L., Fairman,W.A., Wadiche,J.I., Murdoch,G.H.,
            Kavanaugh,M.P. and Amara,S.G.
  TITLE     Functional comparisons of three glutamate transporter subtypes
            cloned from human motor cortex
  JOURNAL   J Neurosci 14 (9), 5559-5569 (1994)
   PUBMED   7521911
REFERENCE   10 (bases 1 to 12006)
  AUTHORS   Shashidharan,P., Wittenberg,I. and Plaitakis,A.
  TITLE     Molecular cloning of human brain glutamate/aspartate transporter II
  JOURNAL   Biochim Biophys Acta 1191 (2), 393-396 (1994)
   PUBMED   8172925
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB465494.2, DA158200.1,
            D85884.1, AL133330.14 and BC047052.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_004171.3.
            
            Summary: This gene encodes a member of a family of solute
            transporter proteins. The membrane-bound protein is the principal
            transporter that clears the excitatory neurotransmitter glutamate
            from the extracellular space at synapses in the central nervous
            system. Glutamate clearance is necessary for proper synaptic
            activation and to prevent neuronal damage from excessive activation
            of glutamate receptors. Improper regulation of this gene is thought
            to be associated with several neurological disorders. Alternatively
            spliced transcript variants of this gene have been identified.
            [provided by RefSeq, Jun 2017].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.249900.1,
                                           SRR1803615.11103.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000278379.9/ ENSP00000278379.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-110               DB465494.2         1-110
            111-562             DA158200.1         107-558
            563-2370            D85884.1           48-1855
            2371-11490          AL133330.14        91036-100155
            11491-12006         BC047052.1         2367-2882
FEATURES             Location/Qualifiers
     source          1..12006
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p13"
     gene            1..12006
                     /gene="SLC1A2"
                     /gene_synonym="DEE41; EAAT2; EIEE41; GLT-1; GLT1; HBGT"
                     /note="solute carrier family 1 member 2"
                     /db_xref="GeneID:6506"
                     /db_xref="HGNC:HGNC:10940"
                     /db_xref="MIM:600300"
     exon            1..609
                     /gene="SLC1A2"
                     /gene_synonym="DEE41; EAAT2; EIEE41; GLT-1; GLT1; HBGT"
                     /inference="alignment:Splign:2.1.0"
     CDS             593..2317
                     /gene="SLC1A2"
                     /gene_synonym="DEE41; EAAT2; EIEE41; GLT-1; GLT1; HBGT"
                     /note="isoform 1 is encoded by transcript variant 1;
                     glutamate/aspartate transporter II; solute carrier family
                     1 (glial high affinity glutamate transporter), member 2;
                     excitatory amino acid transporter 2; sodium-dependent
                     glutamate/aspartate transporter 2; excitotoxic amino acid
                     transporter 2; glutamate transporter-1; human brain
                     glutamate transporter"
                     /codon_start=1
                     /product="excitatory amino acid transporter 2 isoform 1"
                     /protein_id="NP_004162.2"
                     /db_xref="CCDS:CCDS31459.1"
                     /db_xref="GeneID:6506"
                     /db_xref="HGNC:HGNC:10940"
                     /db_xref="MIM:600300"
                     /translation="MASTEGANNMPKQVEVRMHDSHLGSEEPKHRHLGLRLCDKLGKN
                     LLLTLTVFGVILGAVCGGLLRLASPIHPDVVMLIAFPGDILMRMLKMLILPLIISSLI
                     TGLSGLDAKASGRLGTRAMVYYMSTTIIAAVLGVILVLAIHPGNPKLKKQLGPGKKND
                     EVSSLDAFLDLIRNLFPENLVQACFQQIQTVTKKVLVAPPPDEEANATSAVVSLLNET
                     VTEVPEETKMVIKKGLEFKDGMNVLGLIGFFIAFGIAMGKMGDQAKLMVDFFNILNEI
                     VMKLVIMIMWYSPLGIACLICGKIIAIKDLEVVARQLGMYMVTVIIGLIIHGGIFLPL
                     IYFVVTRKNPFSFFAGIFQAWITALGTASSAGTLPVTFRCLEENLGIDKRVTRFVLPV
                     GATINMDGTALYEAVAAIFIAQMNGVVLDGGQIVTVSLTATLASVGAASIPSAGLVTM
                     LLILTAVGLPTEDISLLVAVDWLLDRMRTSVNVVGDSFGAGIVYHLSKSELDTIDSQH
                     RVHEDIEMTKTQSIYDDMKNHRESNSNQCVYAAHNSVIVDECKVTLAANGKSADCSVE
                     EEPWKREK"
     exon            610..749
                     /gene="SLC1A2"
                     /gene_synonym="DEE41; EAAT2; EIEE41; GLT-1; GLT1; HBGT"
                     /inference="alignment:Splign:2.1.0"
     exon            750..902
                     /gene="SLC1A2"
                     /gene_synonym="DEE41; EAAT2; EIEE41; GLT-1; GLT1; HBGT"
                     /inference="alignment:Splign:2.1.0"
     exon            903..1153
                     /gene="SLC1A2"
                     /gene_synonym="DEE41; EAAT2; EIEE41; GLT-1; GLT1; HBGT"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1322
                     /gene="SLC1A2"
                     /gene_synonym="DEE41; EAAT2; EIEE41; GLT-1; GLT1; HBGT"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1449
                     /gene="SLC1A2"
                     /gene_synonym="DEE41; EAAT2; EIEE41; GLT-1; GLT1; HBGT"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1683
                     /gene="SLC1A2"
                     /gene_synonym="DEE41; EAAT2; EIEE41; GLT-1; GLT1; HBGT"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..1878
                     /gene="SLC1A2"
                     /gene_synonym="DEE41; EAAT2; EIEE41; GLT-1; GLT1; HBGT"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..2013
                     /gene="SLC1A2"
                     /gene_synonym="DEE41; EAAT2; EIEE41; GLT-1; GLT1; HBGT"
                     /inference="alignment:Splign:2.1.0"
     exon            2014..2245
                     /gene="SLC1A2"
                     /gene_synonym="DEE41; EAAT2; EIEE41; GLT-1; GLT1; HBGT"
                     /inference="alignment:Splign:2.1.0"
     exon            2246..12006
                     /gene="SLC1A2"
                     /gene_synonym="DEE41; EAAT2; EIEE41; GLT-1; GLT1; HBGT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11985..11990
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC1A2"
                     /gene_synonym="DEE41; EAAT2; EIEE41; GLT-1; GLT1; HBGT"
                     /note="hexamer: AATAAA"
     polyA_site      12006
                     /gene="SLC1A2"
                     /gene_synonym="DEE41; EAAT2; EIEE41; GLT-1; GLT1; HBGT"
                     /note="major polyA site"
ORIGIN      
        1 gggtgatgtc agctctcgac gaaaatagag agggatcgcc tgcaaatccc cagctccggc
       61 ggggctaaac cttgcaatcc ctccctggcc ggcgccgagc cagagcgcag cggcctccac
      121 cgcctcccca ggcgcgcaca cacccgcaca cgcgcacgca cgctcaccgt cctctgccac
      181 cactctctgc tcccgccact cgccgcgccc gcgagccccg cagcaaagca caggtggcag
      241 cggctgcagg ggcgcatcgc cggcgtgcgc cctcctgcag ccctgggcgc atcgctctct
      301 cggggaagcc accctcggag cccccggagc tccccgccaa gcgccatccc cgcgggcgga
      361 ggggagcgcg ggtcgcgcgc cgtggagagc cgggacgcgg attagcgccc gcaggagcct
      421 cctgcgcccg ttgaggcgct aaagggctta ccccggaggc gggtggaagg gcgggcagag
      481 gctcctctta aataccgctc ccggccgcac ttcgcgctca ccccggcgtc cgctttctcc
      541 ctcgcccaca gctgccggat agtgctgaag aggagggggc gttccccaga ccatggcatc
      601 tacggaaggt gccaacaata tgcccaagca ggtggaagtg cgaatgcacg acagtcatct
      661 tggctcagag gaacccaagc accggcacct gggcctgcgc ctgtgtgaca agctggggaa
      721 gaatctgctg ctcaccctga cggtgtttgg tgtcatcctg ggagcagtgt gtggagggct
      781 tcttcgcttg gcatctccca tccaccctga tgtggttatg ttaatagcct tcccagggga
      841 tatactcatg aggatgctaa aaatgctcat tctccctcta atcatctcca gcttaatcac
      901 agggttgtca ggcctggatg ctaaggctag tggccgcttg ggcacgagag ccatggtgta
      961 ttacatgtcc acgaccatca ttgctgcagt actgggggtc attctggtct tggctatcca
     1021 tccaggcaat cccaagctca agaagcagct ggggcctggg aagaagaatg atgaagtgtc
     1081 cagcctggat gccttcctgg accttattcg aaatctcttc cctgaaaacc ttgtccaagc
     1141 ctgctttcaa cagattcaaa cagtgacgaa gaaagtcctg gttgcaccac cgccggacga
     1201 ggaggccaac gcaaccagcg ctgttgtctc tctgttgaac gagactgtga ctgaggtgcc
     1261 ggaggagact aagatggtta tcaagaaggg cctggagttc aaggatggga tgaacgtctt
     1321 aggtctgata gggtttttca ttgcttttgg catcgctatg gggaagatgg gagatcaggc
     1381 caagctgatg gtggatttct tcaacatttt gaatgagatt gtaatgaagt tagtgatcat
     1441 gatcatgtgg tactctcccc tgggtatcgc ctgcctgatc tgtggaaaga tcattgcaat
     1501 caaggactta gaagtggttg ctaggcaact ggggatgtac atggtaacag tgatcatagg
     1561 cctcatcatc cacgggggca tctttctccc cttgatttac tttgtagtga ccaggaaaaa
     1621 ccccttctcc ttttttgctg gcattttcca agcttggatc actgccctgg gcaccgcttc
     1681 cagtgctgga actttgcctg tcacctttcg ttgcctggaa gaaaatctgg ggattgataa
     1741 gcgtgtgact agattcgtcc ttcctgttgg agcaaccatt aacatggatg gtacagccct
     1801 ttatgaagcg gtagccgcca tctttatagc ccaaatgaat ggtgttgtcc tggatggagg
     1861 acagattgtg actgtaagcc tcacagccac cctggcaagc gtcggcgcgg ccagtatccc
     1921 cagtgccggg ctggtcacca tgctcctcat tctgacagcc gtgggcctgc caacagagga
     1981 catcagcctg ctggtggctg tggactggct gctggacagg atgagaactt cagtcaatgt
     2041 tgtgggtgac tcttttgggg ctgggatagt ctatcacctc tccaagtctg agctggatac
     2101 cattgactcc cagcatcgag tgcatgaaga tattgaaatg accaagactc aatccattta
     2161 tgatgacatg aagaaccaca gggaaagcaa ctctaatcaa tgtgtctatg ctgcacacaa
     2221 ctctgtcata gtagatgaat gcaaggtaac tctggcagcc aatggaaagt cagccgactg
     2281 cagtgttgag gaagaacctt ggaaacgtga gaaataagga tatgagtctc agcaaattct
     2341 tgaataaact ccccagcgta tcctatggta actgatgata taaacaagct ttctttaaaa
     2401 aggaaaaaaa tgcgtatatt tctatgttta cttaatctgt tagccgaggc ttagaggagc
     2461 tcttgtgagt cagtgatgac aggcacggtg ctgtgtcttt gccaaataat gctttataac
     2521 cgtctaattt tctcacttgt attattattt gaatggatgc tgctggagga atcagttgga
     2581 attgaagaca cgttcttgcc agcttccctt ttctcccaag atgcagaaat gtggatgctc
     2641 ttttcccagg ggacatgagt aaagcagtgt ggtacactcc agggacttgg gaaaatgagc
     2701 aaacacacag cgtgttattc cttaaagtgt tctccatgtc tcgccttgtt atgcacaaga
     2761 gattctatta aaagcctcta gaagtaactc cccttaaaat gtctagtaaa gcttgcacat
     2821 ggattgatta aaagcaaata cctgtcttag ggaattctga caatttatct tccatatgct
     2881 ctttaagtaa aatgtttcaa agacagtttt aaagggagcc atgctcttaa aggcagttga
     2941 ttaaagaacc tgttacatct ctgccttacc ctgtgtaatc tgtgagaaca atggttgaaa
     3001 tttcaaagta tgtttcatta ttctttctaa atttgataat tgattaggaa gtattttata
     3061 tataaccact gtagatattg acaaaagtaa gagagcacag tcaacataaa gtttaaccag
     3121 agttaaatat tcaaatttat ttatgttcgg tttgccttca cctggtgtag taaaatcaaa
     3181 tgagattatt tggtatgtgt tttgctttgt ttaacccaaa agattatttt ttggttccta
     3241 aagaaaaaga tatttttaat ctgtcaatta tttcagtcat catctcatcg tcctagaaag
     3301 cctggtcatt ttactgtcct cacacatgga agacacttgt gttggctgtg cctcttttaa
     3361 tgtcatctct tgatgaaaca aaaatactgg tatttatcca atatgttgaa agcgttttgt
     3421 tatcttctat caaagcacat aaaaccagtt ataggacaca gttggaagca gagccagtct
     3481 tcctcctcag tcttctcagt agaaaggaac agaaaacaaa catctgtgca aaaccctata
     3541 aaagtgatat tcctatggca gagtccaggc agccttgaac aatgacatgg caacaaagga
     3601 tggctctgta caacttcaca gatggaatct tgtttaaggc tgtgaagttt taaggaataa
     3661 ataatataat tggtgtagca cagtgccttg catgcagtag gcagccaaca aatccttaat
     3721 gattggtaat gcaagctaaa atttttaatg tctctactat ccttttttat tgaataacgc
     3781 acagtatttt cctaaggttg ctgggtttgg gagtaagctt gggacttgaa tcattgagaa
     3841 ataaagcaca tttgactcta agaagtatgt agaaccttgt ttataggtga gaataggctg
     3901 tattatatga aaccagcgga gggatgctga gacagtcatg gtttatagaa tccaaattct
     3961 ctgagatgag ggtgcttatg gacctcaaat agcctttctt atctgctcct tgtgacttct
     4021 ctcttttaaa tcagatacct ttgaaacata caagagacat gagattccag ttgtttttgt
     4081 ctcctaaacc ctagtgattt ttggataagg atcactactg aacagtatta ggcaactttt
     4141 catttttgct tttcaaatga cattggtctt tatttccagt agatgggcag taaacagggt
     4201 cagggtggtc aagggatatg attaagtgta gtagtagaaa atgcattctt tttttttttt
     4261 tttttttaga cagagtcttg ctctgttgct caggctggag tacagtggca cgatctcagc
     4321 tcactgcact gcaatgtcca cctctctagt tcaagcgatt ctcctgtctc agcctcccga
     4381 gtagctggga ctacaggcac atgccaccat gcctggctaa ttttttgtat ttttagtaca
     4441 gatgggtttc attatgttag ccaggatggt ctcgatctcc tgacctcatg atccacccgc
     4501 catggcctcc caaagtgctg ggattacagg cgtgagccac cgtgcccggc cagaaaatac
     4561 attctttaca gcaaatgtta taggttaagg agacagttcc tgggtttagc tgtgaaactg
     4621 tttaggattt tctttaagtg gtgttcccaa gagctttttt ctggtaagtt ggaggctata
     4681 ggcaaagcgt ctaggagctt gttgtttttt gctatggaag gccagctttg ggatctatat
     4741 acatgggcat gtttctcaat ggagtggtca caggattctc ttctcagaac tctgcctgaa
     4801 acctttccgg tgtgttttgt cttcaacttt ttcctagcta tcctattagc ctgtaaatat
     4861 gtcagcagac aatgtgtttg gaagaaacaa ggtgtacaac ttcacaaagc aagttttttc
     4921 tggtctgaga cttactgtgt gcaggaagtc tcgtgagaat atacttagaa aatcacactt
     4981 atctggaaat tcaataagtc atattaaaaa tatacaattt cttgaactca ggactagcct
     5041 gaaaatccaa aatgcatgta gagttgacat gttgacatat gtctatgtcc tctgttttta
     5101 tttaagcctc atttatttct cccttgccat gcccatttgg gattttcttt gcccagcata
     5161 tgtgccataa acagaacaaa tggatccagg gtatacctct aataaccttt atctaaatag
     5221 aatacattta acctgtcaat tatatgtata gatttatcct aattggttca acatatatat
     5281 gaatgcttgt aatagtctgt tactatttga taatgcataa atgttatatt tatatacagt
     5341 tacagcaggc cagaaacata catacactgc atcctgtaag gtctgtaaat cagggcttcg
     5401 gcaatgccat tttgctatag actcattcat gttttttagg aatcagaatc atttcaggag
     5461 aaaaccaact aagtagactt taattcttct ctttctcatt cttgcccttt gagaaaccca
     5521 agtggaatca tcacttaatt acttactcaa attcaatgaa tcaaatccaa tccaatcagt
     5581 gagaagaata gcttatcttt caaggtaata ctttacctta gatctttgag ggcatgcttc
     5641 ccatacaaag agcatcagaa aatcagggca atcatctaga tctcacagat cttcaacata
     5701 ggactctagg ttatgagagc tgaaccacat agctatcagt ttcagttttt acttcctatt
     5761 gagtgtgtag aaacacagcc tatggttttg ctgtctgcgg aagttaccat accctgtaaa
     5821 agacaaatat ttctttctcc tctcgaacca ctagtcagcc tggatattca tgaaagctga
     5881 aagaaggggt gccaaaatgc ctagctcctg gatttagact catggaatat gagaaaggct
     5941 tagggaaaat tcaagtcatg gggagattcc tgctaggtga tgccaggaat ctcaatgacc
     6001 tgttgattag agggtctctg gtcatttcca cttgtgtgtg gagaaataca tcatttgcat
     6061 atgtatctag tcaagaagcc cttttggagc aaagtggttg ggaaactcat ttactgggtc
     6121 caccaaagga aacccaattc tgccatgttt ttgcaaatac tacacgtccc tttgctctca
     6181 atagcaaaag tgatgttata gtttttttta cttttgtcag aatcaaggct tcggccacct
     6241 gtgcaatatt ttcccatgga atgcagcagt aaatccaagt aggtatatcc gtgctcagag
     6301 agaattctcc atagttctga ctcctgaaaa acaagtctca ttagctctca tctagtttga
     6361 tgactaaaag tgcacagcag ttatacccca gtggaagtga tttttctcac ctagctgctc
     6421 tctaactctt acggctatct agttgtctga gctgctggtt tgttttactt attctccaac
     6481 tgaactcatt ccaaaccaag aaaaaggacc aaatgaaatt ttcagttatt atcatacttg
     6541 ctccttgaat aagaatgcta gagtctggag gcagaagaag actaagttaa aaaaaaaaca
     6601 gcctgaagga attctggagt gagttttgta agtctaattg tcaaagaaga taaatggtct
     6661 cagtatctgt agtaatttct ggttaagcct agagggatca ctgagtgcca atccaagcag
     6721 gtggcactag aggcagattg ttctcctagc atagagtgag tttccagctg ctcatgacac
     6781 attttcttat gattttccct aaaagaaaac caaattttct actccacctc ttcctttcct
     6841 atcattccac ttattgttgc tgctttgcta acagccagct tagtaagcca tcacacaagt
     6901 tagtgtgagg cttggtttta aaatagataa cagagaatct tgattaaata gtatccaact
     6961 ttgtagcata acacagctaa ttcaggcaat gacatagaga tgataagaaa caacatggtt
     7021 tggtagaggg aacatttgat ttagactctg cccattttta gctgtatgac ttacataagt
     7081 cattttgtgt ccaagcctca ttttctccca tatgaaaagt gaaggggttg gattaaatga
     7141 ctaaaatccc cttccagccc tatgagccca atgtattatg atctctgctt tgtttccttc
     7201 ttaagaggct tcctactata aaatgtgacc tatttacatt ttaagttgaa gtagcccaca
     7261 ataatgaata atcaatttag atttttctca tctcctttgg gagaaattaa attcaagcct
     7321 ctattcattt gatgttttac aacaagcttc aaagttggcc atgttcattc acagtttgat
     7381 attttgagac accaataaaa gttttttaat aaaagttccc ttgacttaaa ctcaccttcc
     7441 taatagaagt agtgatttgg ctcacggaaa aatgtttcca gagtcaacat gagaggactg
     7501 gatgaaccta cagcctcact caagctgttg catcattgct gctgtagcaa cagagccctc
     7561 tctgaatacc caaaacacac aattttctcc aacaattcta attgcccacc cagtatggga
     7621 atttgagagc ctttggtgaa taaccttgat cactcttttg aaatttggta caacacttgc
     7681 acaatcacgg caactgtttg taaatatctt ttcttctccg ccagtatgta gacatgcaca
     7741 cccattcaca agtaagcccg caaattcatt ttcacatttt cctcttggtt tgtttttatc
     7801 atgggtatca cattgcagga agagatgctc taacttacaa gaatggaatt ttctgactat
     7861 cattgccctc ttcaaggcct gattagtttg tagggtggat gggatacaat gcatggtcta
     7921 caaggtccat gtcaccagcc atgatcttta aacaacctct tgcactttct ggcactgctg
     7981 caatggccca tggtggacac ctctgctgtt tgtccaaact tctgctaaat agagtagtgt
     8041 tggtatttgt tgtgttgtga actcctttgt tgtcagagaa gacttcagct cttcaggttt
     8101 gcaagggcaa gatgtaaagc taggactgaa aggattgggt tttgtttttc ggccggcttt
     8161 tttccattag tgcaaaaaac ctgatgcaag ggcagcaaga taaacacaat ttcttcattg
     8221 acccagataa gaacctgtag aaaagtgaga gatgccccta agttcctatt tcatttatcc
     8281 taaataatat cctgaataac tactgatcag tttttttcta tgtccagcct tgcttcactt
     8341 tccaacctcc aggccaaata ttcattttga tattttacaa aatccttctg gtttttacac
     8401 agggcccttt tgtgctgaga ataaaaccag ctattggaac agacctcact gctttgtaga
     8461 gtcagaagtt gacctagaga atgggtagca acaggtcctc agtttctctg gagacacccc
     8521 caatccttgg gtgagtgttg aaacctgaca actgactgtt agccttgtca tcatcctcac
     8581 tggagatgga gatgttgcag tcggagcctc actccttctg gggcactgtt tcttctcatg
     8641 gcaaccattt tgcctgcctg gaaacagcct gttgcctttg ctagggggca aatacacaca
     8701 ctagccaagg aagttggtcc agcacatctc tgaggtttct cccccatgga cccatggagc
     8761 gaattccctg agataaatga tcagaaagtt ggctgtctgg tggtttaata ctccataaga
     8821 gttacttccc ttatagaaag tcatcctttg gcatctagat ttttctacaa atcaccttaa
     8881 ctccctccct ttctctttct cctggaaaac ctcagcattg catctccatg ttgcacaaca
     8941 cagatcagat tatctggtca ctatagagat ttgtatataa aaaaactact tttttgagga
     9001 ttttgtatat tgtttttcta tttgttttct acagcttgag gaaagagctg gcaaactgtg
     9061 aatctaactt gatcttgttg ccagcagata tattttggct tcctggtaag agtctgtgtt
     9121 accagggaca atatattgcc ctctgatcag tattgcaccc taaatccaca aattcctcca
     9181 gcccagtctt acattttttt aaacaaatcc cctagcttgt ttgctattat ttataaagag
     9241 cattagaaaa tgtatttata gacctggatg ttatatgttt aatattaatt tagcctttaa
     9301 taatgttata ggtttgtaac tattcttcag aaattataaa gaactcagtg tagactgaat
     9361 taatctattt agtatctgta attttgcaga catattttct tacagtattt tctatgtaac
     9421 cacacatgta gaattataac taattagagc acaagaagtt tcttcagcaa tttagagcta
     9481 ccaattgttt cttgactata tagtcacatt tgacaaattt aagaacccag tctttagtga
     9541 tgcaataaac aaaatgaacc attaagaaca aggaattgct taaatccctt agctggtgag
     9601 gatatacatc taaataattc atctttctaa ctcaaggaat ggtgctgatt ttttaaatgt
     9661 ttgacaccag gccttgtttt tccagctgag cattctcatt ttgcttttct ctaagactat
     9721 caaagacaag gtattaatag taggattatt cctagatcag aatgtttcat acattcctaa
     9781 aggtttatgt ggaaattggc ttaggaaaac tttgagtagc agagactgag gatgagtgct
     9841 agagatgaaa tcaggacaga tttgttgcag ttaattcttg ccaagcaaat tagtggtaaa
     9901 tgtcacattg ttatgtgaat tgagcacata tttttaaaga aagtttacaa aaaattttta
     9961 gaaccaacta tgaggcaata ctgtatcact gggggctggg agtgggggct tagaatcata
    10021 ctgaaattgt ttaaaagcag cccaggtagt ttctgtcctc aggtgaataa cagactatat
    10081 aacttccccg aaaggtaaaa cgatagccac tgcagagacg aggtgtcttc cttccaccaa
    10141 atactttctg agatggtggt aggagtagta tttttaccgt ggtttaaaaa gtagtcagtt
    10201 acatagaaag tgtattggta tgtatttgaa cctgctcttc attaaacaaa cagattagat
    10261 gtaccctcga ctggcaataa ttgtatctat tttcaagtac agctagctgt caaagcatga
    10321 agctcttgtg tatacacact gacacttggt tcacgcatga agaacagtgc ctatgcactt
    10381 tgtgtagcta taatgtaggt atctaggtgt aatttcagtg aaatggtgta tagatgtatt
    10441 gtaatttaaa tgtatatgtt atttttggct attcatctaa atgcagtaga catgttgatc
    10501 ggtgttttgc aaacatttct ttttccttct tagataacta gtcgtgaatc atttctcctc
    10561 tttctcagtg tggcttggga atatatatga gtgaagaatt tatctgtgaa tcctttgtac
    10621 tgatgattgt ttgaaagtct gtgtgtgtcc agcacctttg taaatacaca attcagagca
    10681 gggatgggct gggtgtgtgt cctggttctt agtgaaaggt catctcatgt ctgtttaata
    10741 catggtgaat gcaactgtgg aacttttgat tacctagact taggtaggtt tagaatgaga
    10801 acatccatct acagtcctcc tttgcttggt ggattgggct cagaggaaca aaaagttagt
    10861 ctgactctgt gcatattagc atcatgtctt tagagaaagg tcagcctctc tggttgccaa
    10921 atactcatca tgatgctcat gacttaaagg ttctgaggag ccttgtctcc cttggatttt
    10981 tgagtcaggg tacaggaaaa aacattgctg actaactaac tgcaaatgca tctgcaggtg
    11041 aaaccctacg aaagcacagt tctggctata aacttcagag ttctctgtaa aaaacttaga
    11101 gcactagaag cacaggaata gtgagtgtac agcttatgcg gttgtagagg ggcaactgat
    11161 gaacacaggt cccacatata tgagggagta tgacgttctc tacctaatat gttctgtgtg
    11221 catgttttga atgattgaag atgggattaa ctaatgcaag tttacagttg cctcctaaaa
    11281 cacacattct gtataattat cgctaaatac aatgctgtga ggtctatagt tcctgtaacc
    11341 cctttctcct ccccaaggac agagaagaac tagccatgtg ctatagggaa ccctgagtgc
    11401 cctactcttt tcccaagaag ggtaaagcct acaatatcat cagggggcat gaagcacatt
    11461 aatttgcagt ggctgcttca tatgaggaga tatggtggac aggctaattt ttccttgaaa
    11521 atgtggcttc ttcaactcct ttcaaattta ggatggaata cttcctgaaa taaaactggg
    11581 ctttatgcag gattctcttt gaaaattctt gtatgtccag aacaaaagat aaaactaatt
    11641 gtattcctca cattcacaat ccccattggt ctgaagtcac gtagcacaga gcatctatag
    11701 cacatagtgt ttaaagacta atgaatgcaa aaagataaaa tcttcaacta atttttgaat
    11761 tgtttctcat atatgctact agaaaatgcc ttgttgatga agcacatttt gggtagttga
    11821 ggtcttttgt tttcgccttt agctttctaa gctttcttac aatgtggact gattactgta
    11881 acatttcacg tgtaaaataa ctggatattc tttatatact ggaaataacc tgtgaatcca
    11941 atatttcact aagtgtttta acttttgtgt atatatctct catcaataaa tgtggatttc
    12001 aattta
//
