LOCUS       NM_004170               3698 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens solute carrier family 1 member 1 (SLC1A1), mRNA.
ACCESSION   NM_004170
VERSION     NM_004170.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Krishna,G., Pillai,V.S., Gopi,P., Nair,A.S. and Veettil,M.V.
  TITLE     Epstein-Barr virus infection controls the concentration of the
            intracellular antioxidant glutathione by upregulation of the
            glutamate transporter EAAT3 in tumor cells
  JOURNAL   Virus Genes 59 (1), 55-66 (2023)
   PUBMED   36344769
  REMARK    GeneRIF: Epstein-Barr virus infection controls the concentration of
            the intracellular antioxidant glutathione by upregulation of the
            glutamate transporter EAAT3 in tumor cells.
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Xiong,J., Wang,N., Zhong,H.J., Cui,B.W., Cheng,S., Sun,R.,
            Chen,J.Y., Xu,P.P., Cai,G., Wang,L., Sun,X.J., Huang,J.Y. and
            Zhao,W.L.
  TITLE     SLC1A1 mediated glutamine addiction and contributed to natural
            killer T-cell lymphoma progression with immunotherapeutic potential
  JOURNAL   EBioMedicine 72, 103614 (2021)
   PUBMED   34628354
  REMARK    GeneRIF: SLC1A1 mediated glutamine addiction and contributed to
            natural killer T-cell lymphoma progression with immunotherapeutic
            potential.
REFERENCE   3  (bases 1 to 3698)
  AUTHORS   Qiu,B., Matthies,D., Fortea,E., Yu,Z. and Boudker,O.
  TITLE     Cryo-EM structures of excitatory amino acid transporter 3 visualize
            coupled substrate, sodium, and proton binding and transport
  JOURNAL   Sci Adv 7 (10) (2021)
   PUBMED   33658209
  REMARK    GeneRIF: Cryo-EM structures of excitatory amino acid transporter 3
            visualize coupled substrate, sodium, and proton binding and
            transport.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3698)
  AUTHORS   Higashi,Y., Aratake,T., Shimizu,S., Shimizu,T. and Saito,M.
  TITLE     [The neuroprotective role of EAAC1 in hippocampal injury following
            ischemia-reperfusion]
  JOURNAL   Nihon Yakurigaku Zasshi 156 (1), 21-25 (2021)
   PUBMED   33390475
  REMARK    GeneRIF: [The neuroprotective role of EAAC1 in hippocampal injury
            following ischemia-reperfusion].
            Review article
REFERENCE   5  (bases 1 to 3698)
  AUTHORS   Kim,H.B., Yoo,J.Y., Yoo,S.Y., Lee,J.H., Chang,W., Kim,H.S.,
            Baik,T.K. and Woo,R.S.
  TITLE     Neuregulin-1 inhibits CoCl2-induced upregulation of excitatory
            amino acid carrier 1 expression and oxidative stress in SH-SY5Y
            cells and the hippocampus of mice
  JOURNAL   Mol Brain 13 (1), 153 (2020)
   PUBMED   33187547
  REMARK    GeneRIF: Neuregulin-1 inhibits CoCl2-induced upregulation of
            excitatory amino acid carrier 1 expression and oxidative stress in
            SH-SY5Y cells and the hippocampus of mice.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3698)
  AUTHORS   Zerangue,N. and Kavanaugh,M.P.
  TITLE     Interaction of L-cysteine with a human excitatory amino acid
            transporter
  JOURNAL   J Physiol 493 (Pt 2) (Pt 2), 419-423 (1996)
   PUBMED   8782106
REFERENCE   7  (bases 1 to 3698)
  AUTHORS   Shashidharan,P., Huntley,G.W., Meyer,T., Morrison,J.H. and
            Plaitakis,A.
  TITLE     Neuron-specific human glutamate transporter: molecular cloning,
            characterization and expression in human brain
  JOURNAL   Brain Res 662 (1-2), 245-250 (1994)
   PUBMED   7859077
REFERENCE   8  (bases 1 to 3698)
  AUTHORS   Arriza,J.L., Fairman,W.A., Wadiche,J.I., Murdoch,G.H.,
            Kavanaugh,M.P. and Amara,S.G.
  TITLE     Functional comparisons of three glutamate transporter subtypes
            cloned from human motor cortex
  JOURNAL   J Neurosci 14 (9), 5559-5569 (1994)
   PUBMED   7521911
REFERENCE   9  (bases 1 to 3698)
  AUTHORS   Kanai,Y., Stelzner,M., Nussberger,S., Khawaja,S., Hebert,S.C.,
            Smith,C.P. and Hediger,M.A.
  TITLE     The neuronal and epithelial human high affinity glutamate
            transporter. Insights into structure and mechanism of transport
  JOURNAL   J Biol Chem 269 (32), 20599-20606 (1994)
   PUBMED   7914198
REFERENCE   10 (bases 1 to 3698)
  AUTHORS   Smith,C.P., Weremowicz,S., Kanai,Y., Stelzner,M., Morton,C.C. and
            Hediger,M.A.
  TITLE     Assignment of the gene coding for the human high-affinity glutamate
            transporter EAAC1 to 9p24: potential role in dicarboxylic
            aminoaciduria and neurodegenerative disorders
  JOURNAL   Genomics 20 (2), 335-336 (1994)
   PUBMED   8020993
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA643262.1, U03506.1,
            AB008536.1, BQ008494.1 and BQ029108.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_004170.5.
            
            Summary: This gene encodes a member of the high-affinity glutamate
            transporters that play an essential role in transporting glutamate
            across plasma membranes. In brain, these transporters are crucial
            in terminating the postsynaptic action of the neurotransmitter
            glutamate, and in maintaining extracellular glutamate
            concentrations below neurotoxic levels. This transporter also
            transports aspartate, and mutations in this gene are thought to
            cause dicarboxylicamino aciduria, also known as glutamate-aspartate
            transport defect. [provided by RefSeq, Mar 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.20740.1,
                                           SRR1660805.54195.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262352.8/ ENSP00000262352.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-393               DA643262.1         42-434
            394-1321            U03506.1           200-1127
            1322-3036           AB008536.1         1301-3015
            3037-3492           BQ008494.1         16-471              c
            3493-3698           BQ029108.1         19-224              c
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p24.2"
     gene            1..3698
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /note="solute carrier family 1 member 1"
                     /db_xref="GeneID:6505"
                     /db_xref="HGNC:HGNC:10939"
                     /db_xref="MIM:133550"
     exon            1..303
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /inference="alignment:Splign:2.1.0"
     CDS             213..1787
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /note="excitatory amino acid carrier 1; neuronal and
                     epithelial glutamate transporter; sodium-dependent
                     glutamate/aspartate transporter 3; solute carrier family 1
                     (neuronal/epithelial high affinity glutamate transporter,
                     system Xag), member 1"
                     /codon_start=1
                     /product="excitatory amino acid transporter 3"
                     /protein_id="NP_004161.4"
                     /db_xref="CCDS:CCDS6452.1"
                     /db_xref="GeneID:6505"
                     /db_xref="HGNC:HGNC:10939"
                     /db_xref="MIM:133550"
                     /translation="MGKPARKGCEWKRFLKNNWVLLSTVAAVVLGITTGVLVREHSNL
                     STLEKFYFAFPGEILMRMLKLIILPLIISSMITGVAALDSNVSGKIGLRAVVYYFCTT
                     LIAVILGIVLVVSIKPGVTQKVGEIARTGSTPEVSTVDAMLDLIRNMFPENLVQACFQ
                     QYKTKREEVKPPSDPEMNMTEESFTAVMTTAISKNKTKEYKIVGMYSDGINVLGLIVF
                     CLVFGLVIGKMGEKGQILVDFFNALSDATMKIVQIIMCYMPLGILFLIAGKIIEVEDW
                     EIFRKLGLYMATVLTGLAIHSIVILPLIYFIVVRKNPFRFAMGMAQALLTALMISSSS
                     ATLPVTFRCAEENNQVDKRITRFVLPVGATINMDGTALYEAVAAVFIAQLNDLDLGIG
                     QIITISITATSASIGAAGVPQAGLVTMVIVLSAVGLPAEDVTLIIAVDWLLDRFRTMV
                     NVLGDAFGTGIVEKLSKKELEQMDVSSEVNIVNPFALESTILDNEDSDTKKSYVNGGF
                     AVDKSDTISFTQTSQF"
     exon            304..444
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /inference="alignment:Splign:2.1.0"
     exon            445..537
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /inference="alignment:Splign:2.1.0"
     exon            538..652
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /inference="alignment:Splign:2.1.0"
     exon            653..695
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /inference="alignment:Splign:2.1.0"
     exon            696..794
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /inference="alignment:Splign:2.1.0"
     exon            795..979
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1087
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1210
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1405
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1540
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..3698
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3470..3475
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /note="hexamer: AATAAA"
     polyA_site      3492
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /note="major polyA site"
     regulatory      3675..3680
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
                     /note="hexamer: AATAAA"
     polyA_site      3698
                     /gene="SLC1A1"
                     /gene_synonym="DCBXA; EAAC1; EAAT3; hEAAC1; SCZD18"
ORIGIN      
        1 gcaaaactac cgggctggca gggcggcggg cgcggtgcgc gatcccgggt ggcggcggca
       61 acggcggtgg tgacggcggc gactgcagcg gccggctctc acctctcccc tgtgcacccg
      121 catctcgccg cgccgccgag cagccagcag tccccgggtc gcccagccca cgcgcgcacg
      181 gccgagccca gcgcacaata gcggcgacag ccatggggaa accggcgagg aaaggatgcg
      241 agtggaagcg cttcctgaag aataactggg tgttgctgtc caccgtggcc gcggtggtgc
      301 taggcattac cacaggagtc ttggttcgag aacacagcaa cctctcaact ctagagaaat
      361 tctactttgc ttttcctgga gaaattctaa tgcggatgct gaaactcatc attttgccat
      421 taattatatc cagcatgatt acaggtgttg ctgcactgga ttccaacgta tccggaaaaa
      481 ttggtctgcg cgctgtcgtg tattatttct gtaccactct cattgctgtt attctaggta
      541 ttgtgctggt ggtgagcatc aagcctggtg tcacccagaa agtgggtgaa attgcgagga
      601 caggcagcac ccctgaagtc agtacggtgg atgccatgtt agatctcatc aggaatatgt
      661 tccctgagaa tcttgtccag gcctgttttc agcagtacaa aactaagcgt gaagaagtga
      721 agcctcccag cgatccagag atgaacatga cagaagagtc cttcacagct gtcatgacaa
      781 ctgcaatttc caagaacaaa acaaaggaat acaaaattgt tggcatgtat tcagatggca
      841 taaacgtcct gggcttgatt gtcttttgcc ttgtctttgg acttgtcatt ggaaaaatgg
      901 gagaaaaggg acaaattctg gtggatttct tcaatgcttt gagtgatgca accatgaaaa
      961 tcgttcagat catcatgtgt tatatgccac taggtatttt gttcctgatt gctgggaaga
     1021 tcatagaagt tgaagactgg gaaatattcc gcaagctggg cctttacatg gccacagtcc
     1081 tgactgggct tgcaatccac tccattgtaa ttctcccgct gatatatttc atagtcgtac
     1141 gaaagaaccc tttccgattt gccatgggaa tggcccaggc tctcctgaca gctctcatga
     1201 tctcttccag ttcagcaaca ctgcctgtca ccttccgctg tgctgaagaa aataaccagg
     1261 tggacaagag gatcactcga ttcgtgttac ccgttggtgc aacaatcaac atggatggga
     1321 ctgcgctcta tgaagcagtg gcagcggtgt ttattgcaca gttgaatgac ctggacttgg
     1381 gcattgggca gatcatcacc atcagtatca cggccacatc tgccagcatc ggagctgctg
     1441 gcgtgcccca ggctggcctg gtgaccatgg tgattgtgct gagtgccgtg ggcctgcccg
     1501 ccgaggatgt caccctgatc attgctgtcg actggctcct ggaccggttc aggaccatgg
     1561 tcaacgtcct tggtgatgct tttgggacgg gcattgtgga aaagctctcc aagaaggagc
     1621 tggagcagat ggatgtttca tctgaagtca acattgtgaa tccctttgcc ttggaatcca
     1681 caatccttga caacgaagac tcagacacca agaagtctta tgtcaatgga ggctttgcag
     1741 tagacaagtc tgacaccatc tcattcaccc agacctcaca gttctagggc ccctggctgc
     1801 agatgactgg aaacaaggaa ggacatttcc gtgagagtca tctcaaacac tgcttaagga
     1861 aaagagaaac actaatggcc aagtgtacat ttgatttgat atacagacct ccagattatt
     1921 ttctatattt ggattcacag cctttgcgct ctgggttttg ggatttgggt gtggggtaag
     1981 ttgaagggaa atcaatttaa aggaaagttc tattatctgg gttttagaaa ttctataaga
     2041 gacaaagttt ggaagtacat aaagtaataa ctgttagaat taggtaatgg atatgaaaga
     2101 gaaaatgctt tctcatgcat agacaagtgt tttgggtttt taaaaaaaat attctgtcat
     2161 tggttacaaa tttttactca ggctttctat tggcatggat ttcctttgac ctctcacttt
     2221 tttataaatt ataatgcatc taaaccacct gtccccagtt aatgtgccaa aatgtcaatt
     2281 tttaacttat ctccagccaa tttcaaagaa aacagaccag catagttctg caataacagt
     2341 tttaagatgg gcatagggtt tggaagaaag ggagaaggat tcttttttca atgtactgta
     2401 ttgggacgct ggtaactgtt aacccagtgt tcagcataga gctatatata tatatatatg
     2461 tatatattta ttattttcat ataatttgcc agacagagat cagaattgaa ccgtcaatgt
     2521 gaaataaaga gttctccttg tacttgaata ataaccacga ttccaaccca ggtctgcttt
     2581 ggggcttatc agaactcctt tctaaggagc actagaatga gaaatcatgt tgttcgatcg
     2641 tttcacatct gtatatcagc tctaaagcag agatgtatta tggtgatact ccaaggtggc
     2701 atagccattc atttacaact tccagatttg agctgcctgg agggaatcca tatcagctct
     2761 gcataagatt atatacaaag ctgtcactca caaaaggctg gatgtgcttt catccaactg
     2821 gaaggcttta ttcttccaag ttcattcata ctcaaagagg ccagtacttt gccatccttg
     2881 cacttctgtt atcagggccc aaataacagt ggcaagctac caactaagtt gtattttaat
     2941 aaagattcca tgggttgaac aagccacgtt gcagaaaaag agcttcccct aacctgggtt
     3001 gttgcagagt aaatcccacg acataagctg gtatcagtgg ttcgggggaa atagttccat
     3061 tctatgactc ttgtctcctc ctccaggagg actgttctaa ctagtaatct tggccctatt
     3121 cattacatcc tctgcttgtc attctgctaa tttatgaaga tagtttatta tagtctgtac
     3181 ttcagttctc atcttgtaaa taatgcttaa cataaacttg tacttacact gaaatccaaa
     3241 atagtcatgt ttctgcagta ttctgtagcc aacttaaacc tgtgctttca tgtttaagaa
     3301 atgagaaatt gtgccaaaga tagcagaaga gtagataagt gctcagtatt gacgacctac
     3361 atctgaaatc tacaacataa tgatactgaa ttgttatgta aacatcataa atagtaaata
     3421 atgattcaat gtgaatttta aaatgcaaat attgctattg tttataggaa ataaatctaa
     3481 atataaatga aattgaatca gtaatttatc ttgggctaaa tggttctacc ccttactagg
     3541 ttgccccaat tagtggcact agttggcaga gctgttcatg agctgccagt tatcattttg
     3601 gagtcagttt gaaaccagcc tcttaacaca ctgctgttaa ctcataaaag agaagagtgt
     3661 tccatttcag tctcaataaa cacttccctc attctttc
//
