LOCUS       NM_005073               3124 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens solute carrier family 15 member 1 (SLC15A1), mRNA.
ACCESSION   NM_005073
VERSION     NM_005073.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Rafiq,M., Ernst,H.A., Aduri,N.G., Prabhala,B.K., Tufail,S.,
            Rahman,M., Bloch,M.B., Mirza,N., Taylor,N.M.I., Boesen,T.,
            Gajhede,M. and Mirza,O.
  TITLE     Expression, purification and characterization of human
            proton-coupled oligopeptide transporter 1 hPEPT1
  JOURNAL   Protein Expr Purif 190, 105990 (2022)
   PUBMED   34637915
  REMARK    GeneRIF: Expression, purification and characterization of human
            proton-coupled oligopeptide transporter 1 hPEPT1.
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Wang,X., Chen,Y., Wang,Y., Wang,B., Zhang,J. and Jian,X.
  TITLE     Expression, Regulation, and Role of an Oligopeptide Transporter:
            PEPT1 in Tumors
  JOURNAL   Curr Med Chem 29 (9), 1596-1605 (2022)
   PUBMED   35546503
  REMARK    GeneRIF: Expression, Regulation, and Role of an Oligopeptide
            Transporter: PEPT1 in Tumors.
REFERENCE   3  (bases 1 to 3124)
  AUTHORS   Schniers,B.K., Rajasekaran,D., Korac,K., Sniegowski,T.,
            Ganapathy,V. and Bhutia,Y.D.
  TITLE     PEPT1 is essential for the growth of pancreatic cancer cells: a
            viable drug target
  JOURNAL   Biochem J 478 (20), 3757-3774 (2021)
   PUBMED   34569600
  REMARK    GeneRIF: PEPT1 is essential for the growth of pancreatic cancer
            cells: a viable drug target.
REFERENCE   4  (bases 1 to 3124)
  AUTHORS   Wang,Y., Wang,J., Yang,L., Qiu,L., Hua,Y., Wu,S., Zeng,S., Yu,L.
            and Zheng,X.
  TITLE     Epigenetic regulation of intestinal peptide transporter PEPT1 as a
            potential strategy for colorectal cancer sensitization
  JOURNAL   Cell Death Dis 12 (6), 532 (2021)
   PUBMED   34031358
  REMARK    GeneRIF: Epigenetic regulation of intestinal peptide transporter
            PEPT1 as a potential strategy for colorectal cancer sensitization.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3124)
  AUTHORS   Omori,A., Fujisawa,Y., Sasaki,S., Shimono,K., Kikukawa,T. and
            Miyauchi,S.
  TITLE     Protonation State of a Histidine Residue in Human Oligopeptide
            Transporter 1 (hPEPT1) Regulates hPEPT1-Mediated Efflux Activity
  JOURNAL   Biol Pharm Bull 44 (5), 678-685 (2021)
   PUBMED   33952823
  REMARK    GeneRIF: Protonation State of a Histidine Residue in Human
            Oligopeptide Transporter 1 (hPEPT1) Regulates hPEPT1-Mediated
            Efflux Activity.
REFERENCE   6  (bases 1 to 3124)
  AUTHORS   Adibi,S.A.
  TITLE     The oligopeptide transporter (Pept-1) in human intestine: biology
            and function
  JOURNAL   Gastroenterology 113 (1), 332-340 (1997)
   PUBMED   9207295
  REMARK    Review article
REFERENCE   7  (bases 1 to 3124)
  AUTHORS   Thamotharan,M., Lombardo,Y.B., Bawani,S.Z. and Adibi,S.A.
  TITLE     An active mechanism for completion of the final stage of protein
            degradation in the liver, lysosomal transport of dipeptides
  JOURNAL   J Biol Chem 272 (18), 11786-11790 (1997)
   PUBMED   9115234
REFERENCE   8  (bases 1 to 3124)
  AUTHORS   Mackenzie,B., Fei,Y.J., Ganapathy,V. and Leibach,F.H.
  TITLE     The human intestinal H+/oligopeptide cotransporter hPEPT1
            transports differently-charged dipeptides with identical
            electrogenic properties
  JOURNAL   Biochim Biophys Acta 1284 (2), 125-128 (1996)
   PUBMED   8914574
REFERENCE   9  (bases 1 to 3124)
  AUTHORS   Ganapathy,M.E., Brandsch,M., Prasad,P.D., Ganapathy,V. and
            Leibach,F.H.
  TITLE     Differential recognition of beta -lactam antibiotics by intestinal
            and renal peptide transporters, PEPT 1 and PEPT 2
  JOURNAL   J Biol Chem 270 (43), 25672-25677 (1995)
   PUBMED   7592745
REFERENCE   10 (bases 1 to 3124)
  AUTHORS   Liang,R., Fei,Y.J., Prasad,P.D., Ramamoorthy,S., Han,H.,
            Yang-Feng,T.L., Hediger,M.A., Ganapathy,V. and Leibach,F.H.
  TITLE     Human intestinal H+/peptide cotransporter. Cloning, functional
            expression, and chromosomal localization
  JOURNAL   J Biol Chem 270 (12), 6456-6463 (1995)
   PUBMED   7896779
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL708816.1, U13173.1,
            CU445953.1, BC096329.3 and AW449979.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_005073.3.
            
            Summary: This gene encodes an intestinal hydrogen peptide
            cotransporter that is a member of the solute carrier family 15. The
            encoded protein is localized to the brush border membrane of the
            intestinal epithelium and mediates the uptake of di- and
            tripeptides from the lumen into the enterocytes. This protein plays
            an important role in the uptake and digestion of dietary proteins.
            This protein also facilitates the absorption of numerous
            peptidomimetic drugs. [provided by RefSeq, Apr 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U21936.1, BC096329.3 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000376503.10/ ENSP00000365686.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                AL708816.1         4-53
            51-2281             U13173.1           33-2263
            2282-2768           CU445953.1         22-508              c
            2769-2933           BC096329.3         2725-2889
            2934-3124           AW449979.1         16-206              c
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q32.2-q32.3"
     gene            1..3124
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /note="solute carrier family 15 member 1"
                     /db_xref="GeneID:6564"
                     /db_xref="HGNC:HGNC:10920"
                     /db_xref="MIM:600544"
     exon            1..78
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..2201
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /note="macrophage oligopeptide transporter PEPT1;
                     intestinal H+/peptide cotransporter; Caco-2 oligopeptide
                     transporter; peptide transporter 1; solute carrier family
                     15 (oligopeptide transporter), member 1"
                     /codon_start=1
                     /product="solute carrier family 15 member 1"
                     /protein_id="NP_005064.1"
                     /db_xref="CCDS:CCDS9489.1"
                     /db_xref="GeneID:6564"
                     /db_xref="HGNC:HGNC:10920"
                     /db_xref="MIM:600544"
                     /translation="MGMSKSHSFFGYPLSIFFIVVNEFCERFSYYGMRAILILYFTNF
                     ISWDDNLSTAIYHTFVALCYLTPILGALIADSWLGKFKTIVSLSIVYTIGQAVTSVSS
                     INDLTDHNHDGTPDSLPVHVVLSLIGLALIALGTGGIKPCVSAFGGDQFEEGQEKQRN
                     RFFSIFYLAINAGSLLSTIITPMLRVQQCGIHSKQACYPLAFGVPAALMAVALIVFVL
                     GSGMYKKFKPQGNIMGKVAKCIGFAIKNRFRHRSKAFPKREHWLDWAKEKYDERLISQ
                     IKMVTRVMFLYIPLPMFWALFDQQGSRWTLQATTMSGKIGALEIQPDQMQTVNAILIV
                     IMVPIFDAVLYPLIAKCGFNFTSLKKMAVGMVLASMAFVVAAIVQVEIDKTLPVFPKG
                     NEVQIKVLNIGNNTMNISLPGEMVTLGPMSQTNAFMTFDVNKLTRINISSPGSPVTAV
                     TDDFKQGQRHTLLVWAPNHYQVVKDGLNQKPEKGENGIRFVNTFNELITITMSGKVYA
                     NISSYNASTYQFFPSGIKGFTISSTEIPPQCQPNFNTFYLEFGSAYTYIVQRKNDSCP
                     EVKVFEDISANTVNMALQIPQYFLLTCGEVVFSVTGLEFSYSQAPSNMKSVLQAGWLL
                     TVAVGNIIVLIVAGAGQFSKQWAEYILFAALLLVVCVIFAIMARFYTYINPAEIEAQF
                     DEDEKKNRLEKSNPYFMSGANSQKQM"
     exon            79..95
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            96..177
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            178..319
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            320..439
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            440..539
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            540..630
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            631..714
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            715..797
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            798..884
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            885..974
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1019
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1052
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1141
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1223
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1343
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1490
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..1540
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1648
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1757
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1901
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..2009
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2010..3124
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3106..3111
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /note="hexamer: AATAAA"
     polyA_site      3124
                     /gene="SLC15A1"
                     /gene_synonym="HPECT1; HPEPT1; PEPT1"
                     /note="major polyA site"
ORIGIN      
        1 gtcaggtcgg aggagtagca cctgccagga gcacgtcccg ccggcaggtc gcaggagccc
       61 tgggagccgc cgccatggga atgtccaaat cacacagttt ctttggttat cccctgagca
      121 tcttcttcat cgtggtcaat gagttttgcg aaagattttc ctactatgga atgcgagcaa
      181 tcctgattct gtacttcaca aatttcatca gctgggatga taacctgtcc accgccatct
      241 accatacgtt tgtggctctg tgctacctga cgccaattct cggagctctt atcgccgact
      301 cgtggctggg aaagttcaag accattgtgt cgctctccat tgtctacaca attggacaag
      361 cagtcacctc agtaagctcc attaatgacc tcacagacca caaccatgat ggcacccccg
      421 acagccttcc tgtgcacgtg gtgctgtcct tgatcggcct ggccctgata gctctcggga
      481 ctggaggaat caaaccctgt gtgtctgcgt ttggtggaga tcagtttgaa gagggccagg
      541 agaaacaaag aaacagattt ttttccatct tttacttggc tattaatgct ggaagtttgc
      601 tttccacaat catcacaccc atgctcagag ttcaacaatg tggaattcac agtaaacaag
      661 cttgttaccc actggccttt ggggttcctg ctgctctcat ggctgtagcc ctgattgtgt
      721 ttgtccttgg cagtgggatg tacaagaagt tcaagccaca gggcaacatc atgggtaaag
      781 tggccaagtg catcggtttt gccatcaaaa atagatttag gcatcggagt aaggcatttc
      841 ccaagaggga gcactggctg gactgggcta aagagaaata cgatgagcgg ctcatctccc
      901 aaattaagat ggttacgagg gtgatgttcc tgtatattcc actcccaatg ttctgggcct
      961 tgtttgacca gcagggctcc aggtggacac tgcaggcaac aactatgtcc gggaaaatcg
     1021 gagctcttga aattcagccc gatcagatgc agaccgtgaa cgccatcctg atcgtgatca
     1081 tggtcccgat cttcgatgct gtgctgtacc ctctcattgc aaaatgtggc ttcaatttca
     1141 cctccttgaa gaagatggca gttggcatgg tcctggcctc catggccttt gtggtggctg
     1201 ccatcgtgca ggtggaaatc gataaaactc ttccagtctt ccccaaagga aacgaagtcc
     1261 aaattaaagt tttgaatata ggaaacaata ccatgaatat atctcttcct ggagagatgg
     1321 tgacacttgg cccaatgtct caaacaaatg catttatgac ttttgatgta aacaaactga
     1381 caaggataaa catttcttct cctggatcac cagtcactgc tgtaactgac gacttcaagc
     1441 agggccaacg ccacacgctt ctagtgtggg cccccaatca ctaccaggtg gtaaaggatg
     1501 gtcttaacca gaagccagaa aaaggggaaa atggaatcag atttgtaaat acttttaacg
     1561 agctcatcac catcacaatg agtgggaaag tttatgcaaa catcagcagc tacaatgcca
     1621 gcacatacca gttttttcct tctggcataa aaggcttcac aataagctca acagagattc
     1681 cgccacaatg tcaacctaat ttcaatactt tctaccttga atttggtagt gcttatacct
     1741 atatagtcca aaggaagaat gacagctgcc ctgaagtgaa ggtgtttgaa gatatttcag
     1801 ccaacacagt taacatggct ctgcaaatcc cgcagtattt tcttctcacc tgtggcgaag
     1861 tggtcttctc tgtcacggga ttggaattct catattctca ggctccttcc aacatgaagt
     1921 cggtgcttca ggcaggatgg ctgctgaccg tggctgttgg caacatcatt gtgctcatcg
     1981 tggcaggggc aggccagttc agcaaacagt gggccgagta cattctattt gccgcgttgc
     2041 ttctggtcgt ctgtgtaatt tttgccatca tggctcggtt ctatacttac atcaacccag
     2101 cggagatcga agctcaattt gatgaggatg aaaagaaaaa cagactggaa aagagtaacc
     2161 catatttcat gtcaggggcc aattcacaga aacagatgtg aaggtcagga ggcaagtgga
     2221 ggatggactg ggcccgcaga tgccctgacc tctgccccca ggtagcagga cactccattg
     2281 gatggcccct gatgaggaag acttcagaat tgggaactaa accatgaatg ctattttctt
     2341 ttttcttttt cttttctttt tttttttttt tttttttttg agacagagtt ttgctcttgt
     2401 tgtccaggct ggagtgcaat ggcacgatct cagctcactg caacctccgc ctcccaggtt
     2461 caagtaattc tcctgcctca gcctcccgag tggctgggat tagcggcatg caccaccacg
     2521 cccagctatt tttgtatttt tagtagagat ggggtttcac catgttggcc aggatggtct
     2581 cgatctcttg acctggtgat ctgcccacct cggcctgcca aagtgctggg attacaggct
     2641 tgagctaccg cgcccggccg tgaacgctat tttctaagca gccagcagtg aatctaaaac
     2701 tctggaagaa gtcttctgtt tgaaaggctt atttaagcca cacgtacaca cactgtctta
     2761 gagtactgtg agcccacccc acattggtca tcttccctat cacacaaatg atgttatttt
     2821 ggactagctt aattttgaaa tggtaacaaa gtttcctatt ccatactgtt catttctaat
     2881 actcttacga aaactattct aaaggaggca ggagccaagg ccaaaagtga acgtacaggt
     2941 ttgaaatggc tgtgataagg accagctggt attaactgat aactttacct ttgggttttt
     3001 gttattttgt ttttctagtc cctacctgtg tttaaattat ggataactcg aaagacagct
     3061 caggtgaagg ccagtaatga tttttttgaa gtttcaatgg tgtgaaataa atttctgttc
     3121 ttaa
//
